Document N2gvnRvo55G2kKKKjoa9MjK5R

Z-4S REV 2 79 <mB> CONFIDENTIAL 5CC: B. W. Karrh -N-11400 L. J. Papa - 269 R. W. Ubersax - 353 ^ PRAL File - 353 CSTAVUSMCO i m i I.C. - 323 E. I. du Pont de N emours & C om pany INCORPORATED W il m in g t o n . De la w a r e 19898 -4 e-zztri&jis PPD POLYMER PRODUCTS DEPARTMENT EXPERIMENTAL STATION NOV 4 'i!Fq St*Ti<oN November 1, 1982 PERSONAL AND CONFIDENTIAL 'C TO: J. D. DE LAURENTIS, M.D. - CR&DD, BUILDING 301 FROM: S. R. LAAS ^ f . / T ,, /fiO S & S ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 820-969, PRAL Nos. 82-4096, 4099, 4100, 5166, 5168, 5172, 5176-77, 5262, 5269, 5573-75, 5578) Notebook Nos. E29297, E29298 The 14 blood samples, previously reported in letters to you dated 10/19/82 and 10/29/82 as having perfluorooctanoate (C8) concentrations greater than the blank, have been analyzed for C8 by the usual gas chromatographic method ES-567. Results and sample identifications are given in the attached table. The samples are all very low; indeed 3 of the samples are indistin guishable from the blank and 5 are below the quantitation limit. The estimated uncertainty for these analyses is higher than usual due to the very low levels of C8 and the difficulty in integrating the small C8 peak which elutes between the 05 and 06 peaks. If you have any questions regarding these analyses, don't hesitate to call me. Key Words Perf1uorooctanoate GC Blood Analysis JRW 000010 SRL:kab Attach. T K * . . 000228 * -. V a - - - .. -- -- *-- : USEPA 10530 CONFIDENTIAL TABLE I CONCENTRATION OF PERFLUQROOCTANOATE IN BLOOD (a) Sample PRAL No. 82-4096 82-4099 82-4100 82-5166 82-5168 82-5172 82-5176 82-5177 82-5262 82-5269 82-5573 82-5574 82-5575 82-5578 Date Received 7/21/82 7/21/82 7/21/82 9/17/32 9/17/82 9/17/82 9/17/82 9/17/82 9/24/82 9/24/82 10/8/82 10/8/82 10/8/82 10/8/82 Name GC Analysis Date Analyzed 10/25/82 10/25/82 10/25/82 10/22/82 10/22/82 10/22/82 10/22/82 10/25/82 10/25/82 10/25/82 10/26/82 10/26/82 10/26/82 10/26/82 (b) .C8J, yq F/q blood 0.009 n.d. <0.007 <0.007 0.010 0.007 <0.007 0.007 <0.007 0.009 0.007 <0.007 n.d. n.d. (a) Analysis as described in Lab Method ES-557 ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses, (ppm F =0.688 x ppm perfluorooctanoic acid). Estimated uncertainty is +_ 20% relative standard deviation. The lower limit for quantitation is 0.007 yg F/g. The detection limit is '^0.004.yg F/g, but concentrations in that range cannot be well quantitated and are reported as <0.007. None detected (n.d) is reported for samples with [C8j <0.004 ppm. which cannot be distinguished from reagent background. JRW000011 MK i 000229 USEPA 10531