Document N2dxLjvYqDok03OXpxykJaQKy

pR6-0BEE-25 STUDY SUMMARY FOR WORK PERFORMED DURING 1977-1979 EST SUBSTANCE. -- ---- Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8- heptadecafiuoro-, potassium salt, CAS #2795-39-3) Remarks field: The test substance is a white powder of uncharacterized purity. The attached is an overview created in 1979 for studies `completed from 1977 to 1979. Robust summaries have already been submitted for`many of these studies. Others have not been summarized due to the fact new studies exist that supercede these or they are being repeated. STUDIES Water solubility; biodegradation; soil sorption; octanoliwaterpartition coefficient;StaticAcute Toxicity to Bluegill Sunfish (Lepomis `macrochirus), Static Acute Toxicity to Rainbow Trout (Salmo gairdneri); Static Acute Toxicity to Fathead Minnow (Pimephales promelas); Static Acute Toxicity to Daphnia magna; Chronic Flow Through Study on Fathead Minnow (Pimephales promelas) DATA QUALITY Reliability: These studies have been assigned Klimisch rankings = 2. They satisfied criteria for quality testingatthe time performed, but have some deficiencies. In many cases actual concentrations were not measured. The value was solubility) or using nominal determined by concentrations indirect measurement (e.g., (e.g., aquatic toxicity). In some cases, the analytical methodology itself was questionable. Additionally, these studies lack sufficient characterizationofthe test substancepurity. OTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/3/00 01714 -_MICHIGAN STATE UNIVERSITY aJ so SES yay 15, 1993 [PT ------ 3D3eM. CCaoRnmotbpeearrn,ey D. Howell 314g. 2-38-09 Se. Paul Ss1ad1000 Deas Dr. Boveils srEoenmcceolmomsreeedncdoaamtreisnodnwasytifrooenrvsie{fwaoprrcoomvfmiueatungrtestherreesqgeuaaarrldciihtn,yg otPfhleecahnseieneinrdeMivviiTedewucahtlnhiicssttauidlnifeRoser:pmoaractnisdo,n and lec me know 12 T can be of any furcher assiscancer VotIhntidhstohefthahuvereetahrveiierrnc.otonemareeennsdtteaantltiihnoufnsassicuesbssfcaoiinrccdcaiflbnuelgtnyusravesiureprespesosereaotafrrccyhnhoe.uprrMopeFofepsffroahorladepusstcotvtseo3. oobfuenlediancScfoinrsfdoacrnomcaetion Hoynechoanlsfulediaaygs ffoore rfoerviecwheofvomraktepreiratlosraeadndtporedpacaerat1sion$3o,f200r.e0po0re(sthpeieesasodme Bal day consuicacion with Dr.Sowell at MSU, a 4800.00 per dey). xiv, Sincerely, Jfoo SE J dProafessor soya, 5%. Amfmt rerkOst ion c01715 RFienvaileCwoomfpTreechhennisciavle RReeppoorrtt SFuCmm9a5ry An octanol-waterpartition YL using the equation: coefficient of 10 would predicat supercooled liquid solubility of250 log Ko. = -0.862 log 5, + 0.710 cCoounlvderbseel~yif2t8h6e0 wmagt/eLr.soUlsubiinlgittyhoefatbhoevseoleiqduaisti2o8n6, mthgi/sLp,retdhiecntsthaeK.s,upvearlcuooeolfedablioguutid5s0o0l.ubiTlihtey pvraelduiecstesdeevmaliunecsonasriestqeunti,tei.dci.f,fiefretnhtethmaenastuhreedmeKa.suuriesdcovrarleucets,, Ianwdoutlhde emxeapseucratedhiXg.h.eranwdatSe,r solubility, andifthe suspicious. measured solubilityiscorrect I would expecat higher K.n. The values look FdeCfi9n5edicsridteisccalrimbiecdelalse acnonacneinotnriactisounrfsaicmtialnatr. If so, to SDS does it form for example. micelles and exhibit If so, then I'm not a well certain talking about water solubilities is appropriate. should be examined. Rather behavior above and below the CMC t`Toavbalery2dperpeesnednitsngsooinltshoerepxtipoenridmaetantwahlivchais vrirtoiufalaalmyombuenatnloinfegsloeissls,.tThyeopfpesrocieel,ntvsoorbledoufiswagmtoeiern,g `concentration of FC sorption coefficient. 95. This You need should be tdoonmeeaosnurseevtehrealenstoiilrse. saAigl asionr,pitfioFn Cis9o5thiesrma aconndveonbttiaoinnaal `micelle CMC. forming I would nsoutrfeaxcpteacntItliwnoeualrdiseoxtpheecrtmsd.ifferent soil sorption behavior above and below the aApKpr,oprviaaltueenoefss45ofisthriespvoarltueed,biatssehdouolnddbateaofbrtaoimneadnootnhesrevreerpaolrtd(ifrfeefreernetncseoi3l)s. anTdo beevarleulaatteivtehlye constant, or be consistent with K,, values predicted from empirical relationships such as: Tog K,, = 0.904 log K.y - 0.539 (Chiou etal. Environ. S2c1i.)Technal. 17:227- `UmseiansgurtehdevKaleuevoafl4u5e.ofAg1a0,int,hethipsrepdoiicnitesdoKt.h,evianlcuoensiisstaebnocuites2ofwthhiechmeiassmuurcedh Kl.o,weSr,tahnand Kt,h,e vabliouceosnreeenpiorratteidon. faTcthoresKus,inganedmp/iorricSa,l rvelaaltuieonssmhiepassluirkeedthaeroenealasboobvee.inTghiussiesdta roeapsroendaibclte a0ppbreoaicnhcionfstishteenKt,,,soorISd,ovna'tlhueasvaerae aloctcoufrcaoten.fiAdsenmceentiniounseidngabtohveem Kto.praendidcSt,otrheeprorttheindgsseleimke BCE. 001716 Recommendation 1. N(SeeeedmaentahcocduorlaotgeydofeCthieouranmodfiSwcnahtmaeerdstdoilniugbi,oli1tny98a0n-dgoicvteannoiln-wparteevriopuasrtirteipoonrtc.o)efficient 2. Determine the CMC of FC 95, and recognize that its behavior above and below the CMC will be different. 3... Obtain soil sorption isotherms on several soils and calculate K., values from these. 4. Check for consistency between S,, K., and K., values. 001717 femeiarnia TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMUNICATIONS CENTER --201-20N (Important Ifreport isprintedombothside ofpaper, sedtwocopi0TeOCs.) 3715/79 w=Environmental Laboratory: (EE & PC) Fate of Fluorochemicals Final Comprehensive Report: FC 95 TO R. A. Prokoj SE AN Welter 053oH 5 970612643 o11 0= 9362 secunivh CLO wemonos, Thaactastie wSionge]n ote 12 coiamin) iineienion | "aE> arr CURRENT GRIECTIVE: Final Report: Ethnecomppearsisoeds19a7ll7 w-or1k979p.erformed during FEElSuPoCroc-hDeimvi.cal ((Aaqnuaaltyitci)cal) (SDoeugrrsadation) Toxicity ORABSTIACY, (0050rd Toc temas viedo To Gannon Coe Company conti : RD.. LL.. BBoahcoonn - V. Pothapragada RESTb 1) 3MCONFIDENTIAL 601718 z 45 fo, 2. Introduction Tonhefrlautoirooncahleemicfaorlsphearsforbmeeinngdiesncvuisrsoemdmenptraelvioeufsfleyctssl),tudies Tahneionsiucbjescutrfafcltuaonrto)chewmhiiccahlhaosf athpisoternetpioarlt fiosrFCwid95esp(raenad bdaynidsttrhienitbeucrthniraootmnieoinpnallatlthyie.nge,nFvCiarnod95nmeectnchtheimnaigscaltilhnyidsusitmsraitteehsre,iaplboottaihsssiduuosmmeedstically salt of perfluorooctane sulfonic acid. Cg Fyq SOK C 95 Twehiigshtmatoefri5a3l8 aisndantheofcfh-ewmhiictaelposwtdreurc,turheavisnhogwna maobolveec.ular Tahriesas roefporatquactoincsoltioxdiactietsy,allsoialvaisloarbplteioninsftourdmiaetsi,on deingratdhaetion, wtaheterprosboalbublielietnyviranodnmepnatratlitiroinskcoofefFfCici9e5.nts and defines Methods Fhaatveerbeseonlubtihleitsyu,bjebcitodeofgratdeacthniiocnalandrepsooirltsgorgfi. onThsetsuedies "tshpeesceifircepoerxtpseriamnedntsahloulmdethbeodocloongsyusltheadvefobreesnpedceiffiicneddetianils. A) Aquatic Testing Tmhoedelteedstianfgterprothtaotcoldsescurtiibleidzedby fUoSrEPtAhis(1s9t7u5d)y(4y)e,re B) `Acclimation Procedure Ttrhoeutblu(eSgaiflmlo gsauinrfdinsehri()L,epaonmids fmaatchreoacdkimriunsn)ow,s r(aPiinmbeopwhales phraotmcehlearsi)es. useSdtocikn tfhiissh wsetruedyhweeldreinobtfaiibneerdglafsrsomhporlidvinagte . tatank1s4-1f5ilCl.ed wAitdhailcyarpbhoont-ofpielrtieordedofwel1l6 whaotuerrs mlaiignhttaiannedd m8aihnotuarsineddarkt,hrowuitghhouat3t0h-eminaugtcelitmraatnisointiaonnd pteersitoidn,g wpearsiod. T4h8ehofuirssh wpreiroerfetdo Taentdrat-hMrionughoduatilyt,hefotoedstbepeirnigodw.ithheld Fish were so acclimated for 14 days prior to testing. AFcCuOt5eLosthor5t8-3t.eCramrbo(n9-6f-iholutre)redstwaetlilc bwiaotaesrsaoyfskwneorwen pcoemrpfoosrimteidonon ba.. DDeanlneisFatFteingderFisFhishFarHma,tchBerrayd,y, BaNletbirca,skaOhio 01719 c. Tetra-Min - a commercial fish food of known composition -3- was used as the diluent. All glass aquaria (35 x 20 x 20 cm), containing 16.1 of diluent or diluent plus toxicant comprised a study chamber. Fathead minnows (Pimephales promelas) bluegill sunfish (Leponis macrochirue), and rainbow trout (Salmo gairdneri), uniform in size and weight, were tested at each chemical concentration of each test material. Test fish were randomly assigned to various test chambers within 30 minutes following toxicant addition. Test temperatures for the fathead minnow and bluegill sunfish wteroruetmatiesnttsainweedreatcon1d8ucCterdanagte 1147-C1.9', wMohritlaelitthye, rtaeimnpbeorwature, dissolved oxygen level, and pH of all test solutions were measured at 24-hour intervals or until total mortality had occurred. General observations relative to behavioral changes were similarly recorded when 4 appropridte. Organisms used in this study were considered to be generally healthy and free of disease. - Instrumentation used included a teppgrature compensating Orion pH meter, ASTM thermometers ~1C. for temperature monitoring, and a Yellow Springs Dissolved Oxygen meter. A9l5l% caqounaftiidcencsetudliiemsitswewreererecplailccautleadt.ed uLCs,ifgvatlhueesUSwEiPtAh (Duluth) Probit computer program on the 3M TRAC System. C) Aquatic Invertebrates - 48 hour StaticLCgy Daphnia magna, 20 organisms per test, were exposed to FC 95, lot 583 at varying concentrations for 48 hours. First instars were counted and placed in carbon pfriilotreretdo wtehlel awdadtietri,onwoifththceheDmaipchanliaaddmaegdnaa.nd solubilized Test concentrations were 42, 56, 75, 100 and 135 mg/1. LuCs; gvatlhueesUSEwPiAth (9D5ul%utcho)nfiPdroebnicte cloimmpitusterwerperogcraalmcuolnated the 3M Trac System. ResultsThe test protocol utilized for this Thydy was modeled after that described by USEPA (1975) . Table 1 lists water solubility and partition coefficient disatwaatoebrtaisnoeludblues,ing286rapdpinogigb', elsljeidghtFlCy-95l.ipiTdhissolmuabtleerihaalving a partition coefficient of 7-10 . Based on these data thsitoaovleucabnanidleaisbptcepyrriecbcooefindacballaunedcehdeeexmmtpitecehnaratlitcaFailCnnd9tr5heieltswaotebuinilovdoincrsoohnnniomtcpeennbbttei.rtoawcetoeiCnnohcnieonwutafrtaeaecrtttoera.l6) oIrndetrhisofsmyasgtneimtutdhee. ascWrhiebnedappelryroirngitshecondsatiadergeednertoatebde ofnoer FC 95 to this proposed relationship this chemical would be projected 200. to possess a bioconcentration factor of approximately G01720 -3- (Leponis macrochirus), and rainbow trout (Salmo gairdneri), was used as the diluent. All glass aquaria (35 x 20 x 20 cm), containing 16.1 of diluent or diluent plus toxicant comprised a study chamber. Fathead minnows (Pimephales pronelas) bluegill sunfish uniform in size and weight, were tested at each chemical concentration of each test material. Test fish were randomly assigned to various test chambers wtietmhpienrat3u0remsinuftoresthfeolflaotwhienagdtmoixincnaonwt anadddibtliuoeng.ill Tsesutnfish wtreoruetmatiensttsaiwneedreatcon1d8ucCterdangate 11475C1.8, wMohritlaelitthye, ratienmbpoewrature, dissolved oxygen level, and pH of all test solutions were measured at 24-hour intervals or until total mortality had occurred. General observations relative to behavioral changes were similarly recorded when appropriate. Organisms used in this study were considered to be generally healthy and free of disease. Instrumentation used included a teppgrature compensating Orion pH meter, ASTM thermometers -1C. for temperature monitoring, and a Yellow Springs Dissolved Oxygen meter. A9l5l% acqounaftiidcencsetudliiemsitwserweererepclailccautleadt.ed uLCs.,ifigvatlhueesUSwEiPtAh (Duluth) Probit computer program on the 3M TRAC System. C) Aquatic Invertebrates - 48 hour StaticLCs Daphnia magna, 20 organisms per test, were exposed to FC 95, lot 583 at varying concentrations for 48 hours. First instars were counted and placed in carbon filtered well water, with chemical added and solubilized prior to the addition of the Daphnia magna. Test concentrations were 42, 56, 75, 100 and 135 mg/l. utLCgh,se eSMvatTlhrueaecsUSwESiPysAtthe(m9D.5u%lutcho)nfPirdoebnicte cloimmpiutst: ewrerperocgarlacmuolnated Results`The test protocol utilized for this THydy was modeled after that described by USEPA (1975) . Table 1 lists water solubility and partition coefficient data obtained using radiorjgbelled FC-95. This material is water soluble, 286 ppm , Slightly 1ipid soluble having a partition coefficient of 7-10 . Based on these data tito acannabpepreccoinacblluededexttehnatt FiCn 8t5hewoeunlvidronnomtenbti.ocoCnhcieonutreatteal 6) have described an emperical relationship between water Solubility of a chemical and its bioconcentration factor. In this system the ascribed error is considered to be one order of magnitude. When applying the data generated for FC 95 to this proposed relationship this chemical would 200- 001721 be projected to possess a bioconcentration factor of approximately h . --4- TABLE 1 - Physicochemical Characterization of FC-95 PSaorlaumbeitleirty CPaoretfiftiicoinent VTeeistth Method n-octanol/water R28e6sulptpsm 7-10 uFnCd-e9r5 twhaes tfeosutndcotnodibteiocnosmpleemtpelloyyedresis2'.tanTthisto2bi1o/2de-grmaodnatthion schualkteurecsultduerreivbeidodfergormadaacttiiovnatesdtudsyluudtgieliizneodcumliacroobbtiaainledtesftrom TtwhienwaCsitteiestrMeeattmreontplasnytsst.emsDuorfinCghemtohleitpee,riDoedcaotfurt,hisandstutdhye, adevsetlroapi,n ohfencmeicrtohbiessmawtheirchialcouwoludlddebgeradeexpFeCct-e9d5 did not to persist . biyn mtihceroebnivailroncmaetnatbolfiosrm.extended periods of time unaltered Iinf wtohreksiengmatwietrhialfsluodroocdheegmriacdaelse,ithietrhacshembieecnallsyuggorestbeidolotgh-at hiacvaellynevtehratoobnseervdeedgratdhaetifoonrmaptrioodnuctofwoFuCl-d95bebyFCe-i9t5h.er oVfe these aronuttiecsi;pattehderetfoorien,crtehaeseeansviraonrmeesnutlatlofletvheelseoxfogeFnCo-u9s5 parreoduncottion `of this material. S(orialngesor1p4t.i6o-n27.s0t)udioefs thheaveFCsh9o5wnis thaadtsorabpepdroxtiomattheelyBri1l8l.8% sCaonmdpyletleoamdessoiolrptuisoend oifn tthheeseFCs9t5udwiheisch(Thaabdlebe2e)n(3)a.dsorbed (toTasboliel2)w.as aMocbciolmiptlyishoefdFCwi9t5hyign sthrceaelcu(l3a)teddesourtpitliiozningtrials tahree scocnhveemreteddevitsoeda cbyonsHtaamnatk,er'K',',whewrheibcyh ardesfolrecpttsionthecoeofrfgiacniiecnts mcoobnitlenittyofoftahegrsooiulp. ofHpaemsakteircideshacsoulsdhowbne tdheattermtihenedreliamtive mthoibsilefatsohioinmmoabnidlethmatateariraellsatiwvoeuldranrkeisnugl,t. movIinngapfprloyimnghighly mmtohobiriselemtoebswtih,leenFCcthoan9n5parPhaearddaqatuoaKClhlovK__failnube2e0no,,f0004X5o,anbd1e2.is8nl.gighctTolhnyessisdleerdssaabtlay wienadki,catheenctehatthethocsoemplfeotrecesdewshoircphtiobninodfmFaCte9r5iatlo saodislorabreed tios htihgehlsyoimlobainlde.s(e3c)o.ndarily the fact that this material Results of (Table 3). the acute `Based on satqautaitcic atgouaxtiiccitytesctrsitearrieataebsutlaabtleidshed vbyertNeIbOrSaHteFsC a85ndwoiunlvderbteebrcaotnesiudteirleidzesdligihntltyhistoxtiecst(tg)).the Tt is noted that the invertebrate data correlate well 001722 i. Cs. tswphieetcrhieeftsoh.roes,eThdoinasetamoaobybstearcivonanestdiidoenwrhehnDasapthbenesietaningmmaagbdnoeathrdewpaaetaratmeadswlayt&,er wwviaalltliedrbepvreerudttieicbltrioazrteedofstpoeccvhiaeelmsiidcaa(tl9el,1y0t)hi.insducAcoendscteatptotix.sitciictayl taonwaalrymsis aEngdg-ftrhyeirstruedsieusltswerceomcpornitsreacTtaebdlesto 4 EaGn&dG were undertaken to assess the effect of B,ionTohmeisces 13C FC 95 Lsatbuodriaetsory, at sublethal y(loTeuvanebgllseofo4)n.ahsaptItecchiaibesislgieatnryee,raqlusliuytrveiavscaeclne,spittewdieviegthhtaitnadnitdhceatloeimrmgsmtahotfurcechhaeonmvgiecsally atihnFedCupcee95rdcectnootxniccesinuttryrv.aivtailoInnwaotsfhes1pt.9aartmaigms/etlti.ecrasllTyuhnedrereredumcaieindnviens(gtPi.0gt5ea)sttioatn rpaersapmoentseirbslewefroer uthneaffiencctreedasebdy mtohritsalcihteymicoabls.erveTdheamtecthheanism bhaisgehdeston tetshtesecosntcuednitersa,tioanlthcoaunghnotit acics urtaotebley nboeteddettehratmined hatchability was not affected at this dose level. wIanstmhaedeconthtartacttiheng filsahborinatotrhye's1.9 rmegpo/rlt,tantkheweorbeserevxahtiibointing Astrseismsilabrehaovbisoerr;vateirornatwiacssmwaidmmeinogf, adafrekwenfeidshcoilnortahteio1n..0 mcgu/mlulattainvke:suggesting that the toxic action of FC 45 is aBas3e0d-daoyn ethxeporsuerseulttso o1f.9tmheg/lhi1g1foCpatFhCol9o5gidcidalnoetxamcionnattriiobnu,te to abnormal histopathology (Table 5). TABLE 2 - Soil Sorption Test on FC 95 Parameter Test Solution S011 Sorption Adsorption Vater Desorption Water Koo Results 18.8% 1a0d0%sorobfedamount 45 01723 Poel _6- TABLE 3 FC 95: 96 Hour Acute Static Testing Limite Test Organism | n9g6/h1LCg, mlgo/wler Upper ng/1 Fathead Minnow 5317.6 1268.4 5560.2 Bluegill Sunfish 8 62 7a Rainbow Trout 1 8.6 12.5 B. Invertebrate 48 Hour Static Test Daphnia Magna 4509.2 4328..87 5566.6 : 001724 Coe a. TABLE 4 PFLerenyrgcte(hnPtiaamgneedphaAHlavetescrha,pgreoPmWeeerltcaesnW)teiadgguhertiSnougfrviFEvgaaptlgh,seuardMeeManitnonTootwal Varying Concentrations of}3C FC 958+ 30 Days Post Hatch Concentration Hatch Survival Total Length Wet Weight mg/1 % % om ne 19 95 42 20.5 72 1.0 2.5 86 20 62.5 0.45 ~ 96.5 20 20.5 66 0.28 95.5 0 21 ea 0.12 or.5 os 20 63 Control 08 03 to 2 61.5 Solvent Control 89 100 21 64.5 "Work performed by EG&G Bionomics Laboratory, Inc. PS3Mu,nmaStr.y Ptaaubll,e sasubmpairtttedoftofiEnanlvirreopnomretn.tal Laboratory, Significantly reduced at P = 0.05 : TABLE 5 H(iPsitnoepgahtglheoslogpircoamlelaEsx)ameixnpaotsieodn FC. 95% o3f0 dFaaythetoad. 1M.i9nmngo/w1l|%.c Test Material Control Y4crc 95 ONbusmebrevratofions 10 . 10 HistoFpiantdhionlgosgical 91//1100 bfaactttyerilailvergilclhandgiesease 9/10 tissue autolysis 28//1100 nfoartmtayl liver change "Work performed by EG&G Bionomics Laboratory, Inc. . PSStu.mmaPrauyl,tabalsepasrutbmiofttefdinatlo rEenpvoirrto.nmental Laboratory, 3M, 601725 bo he. cBoontthroglr,oup9s/10o,f aonrdgandiossmesd autolysis in the control oerxghainbiistmesd 8f/a1t0t.y lEivveirdenchcaengoefs,{issue group is indicative of a delay oifn htihsetoolrogagniicsaml. preparation of tissues following death Discussion Tshoeurcperimfaorry alplurpeonsveiroonfmetnhtiaslredpaotrat geisnertaotepdrovriedleatiangsintgole rFiCsk9.5 and to provide an analysis of potential environmental wThheichenvcianronbmeendtepmiacytedbeasconfoslildoewrse:d to be. a closed system NL Sol we? mIat,y ethsetraebfloirseh, anfoelqluoiwlsibtrhiautm,chreemmiacialnswietnhtienrinagstihnigsleseynsvtiermonment, or impact on all phases of the depicted cycle. vans NG, om "oIfn FtChe95speinciftihce cfaoslelowaitnghamnadnnweer:can represent the impact No Photodegradation Mr Voplatrility? Soil No Biodegradation Rate? Ne a Toxic to Fry 1.9mg/l ---- Water Ad1s9o%rbed --i ----, -- Sediment pd 286 ppm J Projected to minimally bioconcentrate, low lipophilicity pAsrimsacrhielmyatibcyalmleyansreopfrestehnetewdatearbwoavyesFCand95seecnotnedrasritlhye evinavironment atdhseortberrpeesrtmrainaelntleycostyosteeimthearndsoaitlmosapnhde/roer. sedFiCme9n5t.doeCsomnpotlete mdeosdoerlpg)i.on oIft FiCs u8n5knforwonm w5h0e11thedridoroccnuort tihnisthmeatlearbioarlatovroylatilizes, tahaftactFCwh9i5chdoeasssunomtesunidmeprogrotapnhcoetoilnylsiigsh.t of thBeasefdindoningthe sfionrkegofoirngFCit 95appaenadrsaqutahtaitcwoartgearnwiasymssaarreetthheeeinnvtierrommemdeinattael (0472 Sal . . areccteipotnorosf. FCIt95imsayinbethefouanqdu.atic area that a possible deleterious X Agsrynwoeterde arbeocvoer,dedredatuceFdC s95urvciovnaclentrraatteisonosf offath1e.a9dppmmi.nnow h0The.en1c9enopptmhe.effeacTbthoevseleenvoedltaetdaofmlaeFyvCels9s5ermovafeyFibCnea95apsmrsaueymdeidcitmiptvaoectbefoansahpipaorno:ximately rhautmeasn offoodthessoeurocregabnyissmisg.nificantly reducing the survival oIft FisC 9to5 bmeoreempachcausriazteedlythadtesctrhiebeesnvitrhoenmdeengtraele with this chemical. ocfonrciesnktraatsisoonciated sWeimhpalivsetmiacdemodseelveruasle'dasisnumoputriocnaslcuwlhaitcihonasr:e basic to the 1) Tiontailtspernotdiurcettiyo.n is at Chemolite and it is discharged 2) No FC 95 is removed by the treatment facility. 3) All effluent is discharged to the Mississippi River. 4) FC 95 is discharged uniformly. =" 5) nRoitversubfjleocwtantdo salelasoontahleranpda/roarmetcelrismatairce ccoonnsdtiatnito,ns.hence The formulae used in these projections include: 1) MG/min = . (River Flow, PCroFdMu)c(tCioonnv,erpseiron,agnan1um/ft%)(Tine,min) 2) MG/min X 1440 = WG/Day : 3) Lhs/day = (mg/l X wt. Ibs. HO per gal)(NG/Day) MlioswsifslsoiwppriecoRridverisfl1o0w,00at0 CHFaSs.tings, MN, based on a 10 year RTfihicegkuertreo,stalaCnom5me-eysretcaiirmaaltperdoCdhueemcnitvciiaorlnosnmfeDinigtvuairsleisocnwo.enrceenItpnrraoutvtiiiodlneidziofbnygFCDt.he85sR.e Dtiuonrbitenhge5Mtinhsegs/1ipsesraitipopdHias1tR9i7in3vg-es1r,97b8Me,Nl,owtwhheCihleEeEmColthifeotreEFEwCCas9p5rcoawljcaeuscltaectdaeldcf.ourlated wthaeterpercioomdpar1t9m7e8n-t198i3s tihsepreonjveicrtoendmenattal7 nsgi/n1k. forSiFnCce95theit pirsodduectteiromni,nedFCt9h5atwialtl tnhoetpprreesseennttaannd risk. uprnorjeeacstoendablleeveelnsviorfonmental 001727 soa -10- Conclusions Uphahnsydsebireceotnchheedmteietcseatrlmicnaoennddditetihnaovtnisrotnheimmsepnltomaaylteedrpiriaonlp:ecrhtaireasctoefriFzCing85 thite 21)) 3) hiiassswreaastniesrnt-aosnoctltuabntlooel,m/iwca2rt8oe6bripapplamrtdietgiroandatciooenfficient of ~10 45)) mIiinsnnhaoiwgcholnfyrtyramwcoatbseidleresdtiuundcyeB,drilstlourvsaiavnsadtlyatirlsaottaiemcaoslfoliylthesigfnaitfhiecaadnt eFnCoxrte95nw.tas whaNeobnnoorttmhhaeelrephxaiprsoatsmoueprtaeetrhcsoolnowcgeeynrteroabatsdeivroevnresdewlaysat a1tf.h9fiesmcgt/edldose 6) wloeuvelld. have an estimated of approximately 7 ng/1 3 uenndveirroncomnednittailoncso,ncwehnetrreaitnionall FuCnif9o5rmwleyreimnatonufthaectMuirsesdisastipCphiemRoilvietre. and would be discharged 7) mbiaoylongoitcable daemgreatdaabtoiloint,e levels of FC 95. haesncaerneosulitncroefascehemiincaelnviorronmental 8) FpCres9e5,ntunadnerunrtehaesotneastblecoenndviitrioonnmsendteaslcriribsekd. will mot Bibliography 1. Mendaendl', SuA.p,porTte"c,hniJcaanluarRyepo1r7t,. 19"7A9n.alytical Me3thodology 2. Reinoefr,FluEo.Ar.o,carTbeocnhsnic-alIIIR,epoJrutl:y 19B,iod1e97g8r.adation Studies 3. Welsahn,d FSC.K.1,43TeocnhnSioicla,l RFeepborruta.ry A27d,sor1p97t8i.on of FC 95 4. US/EMSPaeAcrrioe-sinMv6ee6t0hr/ot3de-sb7r5af-to0er0s9A,cauntd1e97A5mT.pohxiibciiatnys,TesEtcowliotgihcaFlishR,esearch 5. Unpublished data 6. Chioaun,d CRaordygerT.,L. ViKroghnielrtH:. FrPeaerdt,itDioanvidCoeW,ffiSccihemnedtdianngd, BEnivoiarcocnummeunltaatlionScioefncSeeleacndtedTecOhrngoalnoigcyCh1e1(m5i)c:als4,75-478, 1977. 7. HamaCkheerm,icaJl.s,., `H"uImnatnerHperaelttahtiaonnd o`tfheSoEinlvirLoenamcehnitn,g AExperiments" CChoelmlieccatlionUSAo,f DMiodwlaSncdi,enMtiicfhiicgaPnape4r8s6,40.Vol. 1, Dow 001728 wears yl. 8. Registry of Toxic Effects of Chemical Substances, +76 Edition, ChristenseH.nE,. and E.J. Fairchild, Eds. P.xvand Ci, U.S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, D.C., June, 1976. 9. Welter, A.N., Technical Report: Aquatic FC 214-30 Lot 566, February 16, 1979, Toxicity Studies: - 10. Welter, A.N., Technical Report: Aquatic Toxicity Studies: FC 232 Lot 610, January 17, 1979. $e 11. Todd, J.W., Technical Report: FC 95/Photolysis Study Using Simulated Sunlight, January 9, 1979. : 601729