Document N2JbNym4DyOgry91bB6rLOEdE
MICROBICS MICROTOXC TOXICITY TEST
TEST SUBSTANCE
ldenfity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95 or as a component ofFC-201AF. (1Octanesulfonicacid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was not noted. The testsample isFC-201AF. Currentinformationindicatesitisa mixtureof 3% PFOS, 18.59% diethyleneglycolbutylether,37.81% water,14% ethyleneglycol, 8.25% Acrylicfoamer,9% sodium octylsulfate0,.3% sodium laurylsulfate, 3% triethanolamine6,% sulfonatedalkylethersalts,and 0.05% tolyltriazole.
The followingsummary applies to a mixture with incompletely characterizedconcentrations of impuddes. Data may not accurately refiectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotox@ "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1995 Species: Photobacteriumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotox@) Test organism source: MicrobicsR'eagent. Test conditions:
Diluent:MilliporMeilli-QTwmater Test temperature: Not noted. Stock and testsolutionpreparation:A 1000 mg/L primarystock solutionwas prepared inMilliporMeilli-Qmwater. The pH ofthe stock solutionwas 6.8. An osmoticadjustmentwas made using 200 mg NaCi dissolvedin10 mL stocksoltuion.Appearance oftestsolutions was noted as "clearand colorless"A.lltestsolutionswere made by proportionadlilutions. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blank control Element basis: Percent lightloss
RESULTS
Nominal concentrations: Blank control,62.5,125, 250, and 500 nig/L
I
Element value:
5-minuteEC5o = 154 (141 - 167) mg/L 15-minuteEC50 = 88 (87- 88)mg/L 30-minuteEC5o = 63 (61- 64) mg/L
Element valuesbased on nominalconcentrations.
Remarks: Testingwas conductedon a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplyto thatmixtureand not the fluorochemicaplroportionalone.
CONCLUSIONS
The testsubstance 30-minuteEC50 forPhotobacteriumphosphoreum was determinedto be 63 nig/L,vvitha 95% confidenceintervaolf 61 to64 nig/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking3. Data sheetsforthe30-minuteexposure couldnot be located.Sample puritwyas notpropedy characterizeadnd the studylacksanalyticaclonfirmatioonftheamount offluorochemicaplroportion inthesoluton.
REFERENCES
Thisstudywas conducted by the3M EnvironmentalLaboratoryS,t.Paul, Minnesota,Lab Request number N2964 1995.
OTHER
Last changed: 6/26/00.
06/25/2000
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
LAB REQUEST NO. N2964 REQUESTOR NAME: SBS/SHARILYN DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 08/24/1995 DESC: FC-201AF (L-13652)
LOUSHIN
CONTRACT LAB(S): EXP COMP DATE: 11/03/1995 DATE COMPLETED: 12/02/1995 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176
# 07-2573-9. Results From Activated Sludge Respiration Inhibition Test:
Test Conc. mg/l 1000 1000 (dup) EC50 sab 9/20/95
Percent Inhibition in Respiration Rate
30-min Exp.
3-hr Exp.
0
0
0
0
> 1000
> 1000
BOD and COD will be on a separate lab request. sab 9/20/95
Results from 48-hour Daphnia magna Toxicity Test: No immobilization was seen after 48-hour exposure to 1000 mg/L. sab 9/28/95
Toxicity to the freshwater green algae, Selenastrum capricornutum can be found on lab request N3281. sab 10/3/95
Results from Test Conc. mg/L 62.5 125 250 500 EC50 95% CI GWJ 12/01/95
Microtox Test: Percent
5-Min Exp. 24 46 64 76 154
(141-167)
Light Loss 15-Min Exp. 37 64 78 86 88 (87-88)
30-Min Exp. 50 76 86 92 63
(61-64)
Results from 96-hr Fathead Minnow Toxicity Test:
Test was run as a static-renewal. Beakers were renewed at 48 and 72-hrs of
exposure. This was done because the dissolved oxygen levels dropped below
the required 60% of saturation. GWJ 12/01/95.
Test Conc. mg/L
24-hrs exp.
Percent mortality
48-hrs exp.
72-hrs exp.
96-hrs exp.
100
0
0
5
5
180
0
5
5
10
320
5
30
35
45
560
100
100
100
100
1000
100
100
100
100
LC50
410
350
345
310
95% CI
(390-435)
(305-395)
(300-400)
(270-370)
% Trim
0
0
5
5
Trimmed Spearman-Karber Statistical Program was use to calculate LC50's.
GWJ 12/01/95.
--------------------------------------------------------------------------------
SAMPLE DATE
CODE
DESCRIPTION
RESULT
MDL or (95% C.I.)
--------------------------------------------------------------------------------
06/25/2000
ENVIRONMENTAL LABORATORY
2
ARCHIVED FINAL REPORT
LAB REQUEST NO. N2964
CONTINUED
--------------------------------------------------------------------------------
SAMPLE DATE
CODE
DESCRIPTION
RESULT
MOL or (95% C.I.)
--------------------------------------------------------------------------------
1
48-HR-EC50 96-HR-LC50 MICROTOX SLUDG-RESP
FC-201AF
Dr Green liq
DAPHNIA ACUTE TEST FISH ACUTE TEST - 96 HOUR MICROTOX - EC50 SLUDGE RESPIRATION - EC50
>1000 MG/L 310 MG/L 63 MG/L >1000 MG/L
(270 370) (61 64)
ENVIRON-NlEiN-T.AL LABORATORY:
TEST SU13STANCIE:
-20 IAF
NIICROTOX
PREP SIIEET
LR-
Date:-loll7li5-
Analyst
-T
1. Preparation of Stock Solution
* 4VfassIVToels.tSubstance:
* Final DilutionVolume: * Diluent:
* FinalConccntra6on of Stock Sof@@o"n:
2. pH Adjustment of Stock Solution-
* InitiaplH ofstockSolution: * pHA djustedto:
pHAdjusted with:
3.OsmoticAdjustmentofioa Soliition
0 smoticAdjuster-,'-
Commen@s-
0
PL
4.A@pp earance o'f OPA Q
sm oticary.AdjustiA StockSolution
CLrci-nme
L
WTLE N2964-3.K5 F@',-20 1 A P
MICROTOX DATA REFC)@Basic Test
-,-St Ti-,ne: 5 minutes
()smotic Ad-@,i-@tment:NaCl
71m1)ElE p
!O/IT
CCINC
A @MM A
r0 .,Dntrol
4 5
@45,33/ 64.70 89.00/ 62.54
9 3 / 48.27
;i9.09/ 46.65
34.01
8i-A.06/ 33.07 87.57/ 21.50
82.89/ 85@1.5/ 82@78/
21.36 14.23 13.53
0.0 O-C)
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13
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FACTOR
0.6907
E@7t@'i l@,3.6973 MG/L (95% CONFIDENCE RANGE:
Ti" i
5 2 s)
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TEST DAT-. TIm-:
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