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FOR DU PORT USE ORLY REVISED Copies to: E. E, Swain, Jr. (3) E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 373-80 MR NO. 3540-001 Material Tested 1) Paint, Dulux Enamel* 2) Lead Naphthanate* Haskell No. 13,257 13,163 Other Codes TfWZlbbl H-624 Study Initiated/Completed 2/4/80-3/14/80 Material Submitted by E. E. Swain, Jr. Fabrics & Finishes Dept. Wilmington ACUTE SKIN ABSORPTION TEST ON RABBITS Procedure: Three groups of male albino rabbits, 10 per group with average body weights approximately the same, were clipped free of hair over the back and trunk area and fitted with plastic collars. Group I, controls, received 1.0 ml of distilled water. Group II, test rabbits, was treated with 1.0 ml of a suspension containing paint, Dulux enamel and 1.2% lead naphthanate. Group III, test rabbits, was treated with 1.0 ml of a suspension containing paint, Dulux enamel and 6.0%'a lead naphthanate. One and a half inch square plastic pads were used to contain the material. The trunk of each animal was then wrapped with a layer of Saran Wrap, Kling gauze bandage and Elastoplast adhesive bandage. After a 6-hour exposure period, the wrappings were removed, All animals received treatments once a day for 10 consecutive days. All rabbits were held for 29 days after the last treatment for blood evaluations. Blood lead levels for the test rabbits were analyzed before treatment and on days 5, 10 (last treatment day), 15 and 39 of the study. The control rabbits were examined before treatment and on days 15 and 39 of the study. Blood levels were analyzed by flame atomic absorption spectroscopy.t a = Lead naphthanate added to base paint containing 0.415% lead naphthanate to equal % indicated-. Results: A few blood lead values appear to be inconsistent with the other determined values, but are listed for completeness. In summarizing the data, these Values are considered to be outliers. DUP040002339 Group I - Controls - Distilled Water Rabbit No, 15030 15031 15032 15033 15034 15035 15036 15037 15038 15039 Body Weight Initial Final Wt. (gm) Wt. (gm) Day 0 Day 29 2562 2409 2380 2367 2563 2170 1853 2400 2535 2283 3215 2955 2900 3028 2850 2805 2587 2628 3007 3054 Blood Lead Level (pg/l) Initial Day 15 Day 29 ND ND ND ND ND 124 ND ND ND n d ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND x=2352.2 x=2902.9 Group II - Paint, Dulux Enamel with 1.2% Lead Naphthanate Rabbit No. Body Weight Initial Final Wt. (gm) Wt, (gm) Day 0 Day 29 Blood Lead Level (jug/1) Treatment Days Post- Treatment Days Initial Day 5 Day 10 Day 15 Day 29 Day 39 15010 15011 15012 15013 15014 15015 15016 15017 15018 15019 2310 1963 2325 1941 2170 2212 1882 2367 2422 2427 3375 2772 3245 3530 2735 3182 3085 2910 3012 2535 ND 253 175 311 54 132 28.5 154 163 179 117 60 31,5 384 158 199 157 76 ND 215 252 317 138 90 ND 435 337 255 191 134 ND 436 204 344 100 76 ND 105 - 358 297 100 32 ND 519 206 300 223 84 ND 365 ND 222 241 69 ND 404 ND 208 97 68 x=2392,8 x=3038.1 x=327.8 232.0 +136.8 +77,7 263.9 +57 142,0i +60 82.0 +31 DUP040002340 2 Group III - Paint, Dulux Enamel with 6% Lead Naphthanate Rabbit No, Body Weight Initial Final Wt. (gm) Wt. (gm) Day 0 Day 29 Blood Lead L.evel (jag/1} Treatment Days Post-Treatment Days Initial Day 5 Day 10 Day 15 Day 29 Day 39 15020 15021 15022 15023 15024 15025 15026 15027 15028 15029 2287 1898 2173 2151 2444 2647 2266 2052 2441 2250 3058 2741 2955 3008 2625 2938 2900 2411 2390 2730 ND 348 122 266 281 119 ND 490 252 455 306 169 ND 104 ND 631 220 120 ND 88* ND 491 268 161 ND 363 288 502 304 113 ND 481 636 493 270 87 ND 342 303 365 218 80 ND 307 393 392 364 ND ND 479 350 167 254 150 ND 639 471 1156 284 137 x=2362 x=2876 x=364. 9 x=352. 0 x491.18 x=277 .0 x=114 +172.Ci +153.9 +267.6 +43 +49 ND = None detected (Minimum detectable limit ?= 25 p-g/1) * = Sample spilled, no correction. Clinical Signs: Weight loss was sporadic in both tests groups and the controls. Rabbit 15026, Group III, had moderate-severe eye discharge from the right eye and severe discharge from the left eye on the 14th day of the study. Throughout the study, there was a problem of the paint adhering to the skin and to the plastic pads used to contain the paint. Because of this, glass watch covers were substituted for the plastic pads on the 4th day of treatment. Rabbits 15013 and 15025 had sores and cracks on their skin due to the paint by the 5th day of treatment- By the 7th day of treatment, there was generally some cracking of the skin throughout both test groups, II and III. It can be seen that the lead was absorbed from the skin by comparing values from day 0 to day 5 in both groups. Blood lead values appeared to reach a peak by day 5 and leveled off by day 10 in Group II. These values did not change very much by day 15, However, by day 29 lead values had dropped from a mean of 263.9 to 142,0 jag/1 at day 15. This value decreased to 82 jug/1 at day 39. Group III blood lead values appeared to reach a peak by day 5, though the pattern was not as definite as with Group II. The blood lead values appeared to level off by day 10; and as in Group II, the values had not changed very much by day 15. Again, there was a drop in lead values from a mean of 491.8 p-g/1 at day 15 to a mean of 277 jig/1 at day 29. This value decreased to 114 jug/l with an additional 10 day period (Day 39). 3- - DUP040002341 Summary: Paint Dulux Enamel containing 1.2% and 6% lead naphthanate was applied under occlusion for 10 consecutive days to the skin of 2 groups of 10 rabbits, respectively, for 6 hours per day to determine the amount of lead absorbed during and up to 29 days after exposure. When paint, Dulux Enamel containing 1.2% and 6% lead naphthanate was administered topically to rabbits lead from the paint was absorbed and detected in blood. By comparing the blood lead values for the 1.2% and 6% groups throughout the study, a dose response relationship can be observed where the absorption is greater in the 6% group by approximately a 1.5 fold increase, expressing less than a linear absorption pattern. This study also demonstrates that these values peak and once exposure is terminated, they level off and with time decrease. * Composition: Paint, DuluX Enamel (H-13,257) Titanium dioxide Pigments Soya lecithin Alkyd Resins Butylated melamine-formaldehyde Guaicol C Linseed Oil 6% Cobalt Naphthanate 24% Lead Naphthanate 6% Manganese Naphthanate Toluene Butyl alcohol Naphtha Mineral Spirits Hydrocarbon Solution Xylene Arom. Hydrocarbon 150-190C Arom. Hydrocarbon 182-219C 15.7% 0.16 0.19 31.77 0.34 0.03 0.02 0.20 0.415 0.034 0.27 0.27 11.41 10.3 8.05 2.76 12.5 5.3 Lead Naphthanate (H-13,163) 24% t Blood lead analyses were conducted by D. J. Kasprzak, Research Chemist, Haskell Laboratory (HLR No. 241-80). Graphs of blood lead values for each individual rabbit in the test groups are attached. Report by Polly Ashley Biochemist Approved by: .0J Gerald L. Kennedy' ~ Chief, Acute Investigations Section PA:vim Study Director: 0. L. Dashiell Date Issued: July 16, 1980 N.B. E--21214, p. 1 Report No. 373-80 Date Reissued: September 9, 1980 4- - DUP040002342 Copies to: E. E. Swain, Jr, (3) E, X. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO, 373-80 MR NO. 3540-001 Material Tested 1) Paint, Dulux Enamel* 2) Lead Naphthanate* Haskell No. 13,257 13,163 Other Codes #93-21667 H-624 Study inltiated/Completed 2/4/80-3/14/80 Material Submitted by E. E. Swain, Jr. Fabrics & Finishes Dept. Wilmington ACUTE SKIN ABSORPTION TEST ON RABBITS Procedure: Three groups of male albino rabbits, 10 per group with average body weights approximately the same, were clipped free of hair over the back and trunk area and fitted with plastic collars. Group I, controls, received 1.0 ml of distilled water. Group II, test rabbits, was treated with 1.0 ml of a suspension containing paint, dulux enamel and 1.2% lead naphthanate. Group III, test rabbits, was treated with 1.0 ml of a suspension containing paint, dulux enamel and 6.0a lead naphthanate. One and a half inch square plastic pads were used to contain the material. The trunk of each animal was then wrapped with a layer of Saran Wrap, Kling gauze bandage and Elastoplast adhesive bandage. After a 6-hour exposure period, the wrappings were removed. All animals received treatments once a day for 10 consecutive days. All rabbits were held for 29 days after the last treatment for blood evaluations. Blood lead levels for the test rabbits were analyzed before treatment and on days 5, 10 (last treatment day), 1.5, 20 and 39 of the study. The control rabbits were examined before treatment and on days 15 and 39 of the study. Blood levels were analyzed by flame atomic absorption spectroscopy.t - Lead naphthanate added to base paint containing 0.415% lead naphthanate to equal % indicated. , Results; A few blood lead values appear to be inconsistent with the other determined values, but are listed for completeness. In summarizing the data, these values are considered to be outliers. 1 DUP040002343 Group I -- Controls - Distilled Water Rabbit No. Body Weight Initial Final Wt. (gm) Wt. (gm) Day 0 Day 29 Blood Lead Level (ug/1) Initial Day 15 Day 29 15030 15031 15032 15033 15034 15035 15036 15037 15038 15039 2562 2409 2380 2367 2563 2170 1853 2400 2535 2283 3215 2955 2900 3028 2850 2805 2587 2628 3007 3054 ND ND ND ND ND 124 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND x=2352.2 x=2902.9 Group II - Paint, Dulux Enamel with 1,2% Lead Naphthanate Rabbit No. Body Weight Initial Final Wt. (gm) Wt. (gm) Day 0 Day 29 Blood Lead Level (ug/1) Treatment Days Post--Treatment Days Initial Day 5 Day 10 Day 15 Day 29 Day 39 15010 15011 15012 15013 15014 15015 15016 15017 15018 15019 2310 1963 2325 1941 2170 2212 1882 2367 2422 2427 3375 2772 3245 3530 2735 3182 3085 2910 3012 2535 ND 253 175 311 54 132 28.5 154 163 179 117 60 31.5 384 158 199 157 76 ND 215 252 317 138 90 ND 435 337 255 191 134 ND 436 204 344 100 76 ND 105 358 297 100 32 ND 519 206 300 223 84 ND 365 ND 222 241 69 ND 404 ND 208 97 68 x=2392.8 x=3038.1 x=327.8 232.0 +136,8 +77,7 263.9 +57 142.0 +60 82.0 +31 2- - DUP040002344 Group III ~ Paint, Dulux Enamel with 6% Lead Naphthanate Rabbit No. 15020 15021 15022 15023 15024 15025 15026 15027 15028 15029 Body Weight Initial final Wt. (gm) Wt, (gm) Day 0 Day 29 2287 1898 2173 2151 2444 2647 2266 2052 2441 2250 3058 2741 2955 3008 2625 2938 2900 2411 2390 2730 x-2362 x=287 6 Blood Lead Level (mg/1) Treatment Days Post"-Treatment Days Initial Day 5 Day 10 Day 15 Day 29 Day 39 ND 348 122 266 281 119 ND 490 252 455 306 169 ND 104 ND 631 220 120 ND 88* ND 491 268 161 ND 363 288 502 304 113 ND 481 636 493 270 87 ND 342 303 365 218 80 ND 307 393 392 364 ND ND 479 350 167 254 150 ND 639 471 1156 284 137 x~364,9 x=352.(3 x-491 .8 x=277 .0 x=114. +172.0 +153.9 +267.)6 +43 +49 ND ~ None detected (Minimum detectable limit = 25 ug/1), * = Sample spilled, no correction. Clinical Signs: Weight loss was sporadic in both tests groups and the controls. Rabbit 15026, Group 111, had moderate-severe eye discharge from the right eye and severe discharge from the left eye on the 14th day of the study. Throughout the study, there was a problem of the paint adhering to the shin and to the plastic pads used to contain the paint. Because of this, glass watch covers were substituted for the plastic pads on the 4th day of treatment. Rabbits 15013 and 15025 had sores and cracks on their skin due to the paint by the 5th day of treatment. By the 7th day of treatment, there was generally some cracking of the skin throughout both test groups, XI and III. It can be seen that the lead was absorbed from the skin by comparing values from day 0 to day 5 in both groups. Blood lead values appeared to reach a peak by day 5 and leveled off by day 10 in Group II. These values did not change very much by day 15. However, by day 29 lead values had dropped from a mean of 263.9 to 142.0 ug/1 at day 15. This value decreased to 82 ug/1 at day 39. Group III blood lead values appeared to reach a peak by day 5, though the pattern was not as definite as with Group It. The blood lead values appeared to level off by day 10; and as in Group IX, the values had not changed very much by day 15, Again, there was a drop in lead values from a mean of 491.8 ug/1 at day 15 to a mean of 277 ug/1 at day 29. This value decreased to 114 ug/1 with an additional 10 day period (Day 39). 3- - DUP040002345 Summary: Paint Dulux Enamel containing 1.2% and 6% lead naphthanate was applied under occlusion for 10 consecutive days to the skin of 2 groups of 10 rabbits, respectively, for 6 hours per day to determine the amount of lead absorbed during and up to 29 days after exposure. When paint, Dulux Enamel containing 1.2% and 6% lead naphthanate was administered topically to rabbits lead from the paint was absorbed and detected in blood. By comparing the blood lead values for the 1.2% and 6% groups throughout the study, a dose response relationship can be observed where the absorption is greater in the 6% group by approximately a 1,5 fold increase, expressing less than a linear absorption pattern, this study also demonstrates that these values peak and once exposure is terminated, they level off and with time decrease. * Composition: Paint, Dulux Enamel (H-13,257) Titanium dioxide Pigments Soya lecithin Alkyd Resins Butydated melamine-formaldehyde Guaicol C Linseed Oil 6% Cobalt Naphthanate 24% Lead Naphthanate 6% Manganese Naphthanate Toluene Butyl alcohol Naphtha Mineral Spirits Hydrocarbon Solution Xylene Arom. Hydrocarbon 150-190C Aron. Hydrocarbon 182--219C 15.7% 0.16 0.19 31.77 0.34 0,03 0.02 0.20 0.415 0.034 0.27 0.27 11.41 10.3 8,05 2.76 12.5 5.3 Lead Naphthanate (H-13,163) 24% t Blood lead analyses were conducted by D J. Kasprzak, Research Chemist, Haskell Laboratory (HLR No. 241-80). Graphs of blood lead values for each individual rabbit in the test groups are attached. Report by: PA:vim Study Director: 0. L. Dashiell Date Issued: July 16, 1980 N.B. E-21214, p. 1 Report No. 373-80 Approved by: Gerald L. Kennedy Chief, Acute Investigatziolonxis Section 4- - DUP040002346