Document N285Z8mDMOYj5YyprNwXn35bp
f o r m 8 7 4 7 -1 1-A
ORIGINAL
TECHNICAL REPORT SUMMARY
TO: T E C H N IC A L C O M M U N IC A T IO N S CEN TER - 201-2CN
,im portant -- If report is prin ted on both sides o f paper, send two copies to T C C J
O ivision
CRL - Analytical and Properties Research Laboratory
Project
Trace Fluorochemicals in the Environment
R eport Titla
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IRDC 137-092: FC-95/Monkey
To
J. E . Long
A u th o rlt)
Jon Belisle___________
N oteb oo k Reference
D ata
8/4/78
Dept. N um ber
0502
Project Number
A000006
fteport Number
967
Employee Number!)
88240
No. of Paget Including CoverIheat
SECURITY
O Opan
O f Closed
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Thesaurus. Suggest other applicable terms.)
CURRENT OBJECTIVE:
Requestor: J.E. Long;
3M C H E M IC A L R EG ISTRY
New Chemicali Reported
V*
3 0 No
Request #A69507; Project #9172110004
Central Research Analytical Report Chemical/Analysisi
REPO RT A B S T R A C T : (200-250 words) This abstract information is distributed by the Technical Communications Center alert 3M'ers to Company R&D. It is Company confidential material.
The level of FC-95 was determined In the serum and livers of monkeys from the IRDC subacute toxicity study 137-092.
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CENTRAL ANALYTICAL LABORATORY R ep o rt N o...........69.6.7.......................... D a te _____ &USUS-t- A*. JL928--------
Subject: irdc 137 -0 92: FC-95/M onkey
R e q u e s to r:___J - E ._ L o a g ____
D ept. N am e _.Toxicology. _
R eq u est N o . 5 9 1- - _____
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R ep o rt:
Proj. No.91J2AL0004
Reference 137-087 by IRDC was a 90 day subacute Rhesus monkey toxicity
study of FC-95. Incorrect (too high) feeding levels were used and all animals died within the first few days. The feeding levels were lowered
and the study started again as 137-092. The serum and livers were each individually submitted for analysis. Each group contained 4 monkeys, 2 male and 2 female as follows:
Group I
II III
IV
SERUM ANALYSIS
Dosage Level 0
0.5 mg/kg/day 1.5 mg/kg/day 4.5 mg/kg/day
Survival 4/4 4/4 4/4 0/4
Monkey Blank on Method
Dosage Level -
FC-95 in Serum (ppm)* 2
7355M 7358M 7368F
0 40 0 20 0 15
7463M 7466F
0.5 mg/kg/day
II
150 150
7462M
1.5 mg/kg/day
7500F
II
CD Our newly developed pyrolysis method was used.
Precision is estimated to be 10 - 25%.
250 275
A .R . #6967
2 August 8, 1978
The high levels of FC-95 in the control serum cannot be explained. ' For example, 7355M was run on 2 different days with the same experimental
results. Perhaps a total fluorine should be run on this sample to determine if this level of F is present. See Table below containing liver results for further discussion (Footnote 2).
LIVER ANALYSIS
Monxey
Dosage Level
FC-95 in Liver(mg) FC-95 in Liver(ppm)
7355M 7368F
0 0
3000 1500
520(S&
7463M 7466F
0.5 mg/kg/day
tl
7000 8000
100 100
7462M 7500F
1.5 mg/kg/day
II
45000 40000
650 600
7484M 7502F
4.5 mg/kg/day
II
40000 80000
650 1000
The livers were stored in the refrigerator several months prior to analysis resulting in the separation of some liquid. The high values and physical size necessitates taking only partial livers in which case the same ratio (estimated visually) of solid to separated liquid was taken for analysis. In addition, recovery of FC-95 was estimated from experiments where FC-95 was added to a control liver.
Because of a higher than expected value for the control, a 0.133 g liver sample was analyzed for total fluorine. The fluorine value calculated as FC-95 is equivalent to 11 ppm FC-95 in the liver. As time permits, more work should be done to verify these "high" control levels.
p S c:R.A. Prokop 236-3
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