Document N2747GJnp9pppNvgrBOrxDLo8

^Sr'&uai^SSTTiisiowi The Toxicity of the,Foss Formed by Dropping Pydraui'F9^ Aroclor 1243 and Trlcresyl Phosphate, Upon the Surface of a Heated Inconel (Project 49) by Treon, Cleveland, Shaffer, Cappel, Oahegan, Wagner March 11, 1953 0615621 TOXSTUDIESOI00 h; fP NFIOCNTML Pag# i scope .................................................. . ,.............................. .... 1 Conclusions . . ........................................ ........ 1 Materials.............................................................................. $ Experimental Methode................................... 7 Equipment........................................................................... 7 Volatilisation of th Material* Without Decomposition.................................................... 7 oonsratlon of a Fog From a Heated Inoonel Surface................................................ 9 Sampling and Analysis of Contaminated Air . . 11 experimental Result* , . .......................................................... 19 Exposure to Utxleoomposed Vapor* ....... 19 iixpoeure to Product* Arising Prom Fydrsul M. ' Aroolor 1248, or Trloresyl Phosphate Whan In Contaot with Inoonel at 1250 or 1050 F......................................... .21 Mortality ............................................................. 21 Sign* end Pathology of Intoxloatlon ........... 23 The Conoontratlon* of Identified Decomposition Product* Formed at the Higher and Lower Tanj>eraturea .............................................................. 2$ Discussion . .......................... 28 Supplementary Statement - The Application of Fydraul F-9. Aroolor 1248 and Trlereayl phosphate Open the Skin of Rabbit*...................... ......................... . 31 Experimental Methods ..........................................................31 Experimental Results ............. 32 0615622 TOXSTUDIESOI01 p 0 N F /-D E N T J A L ' Soquonoo Of Tables 1. The Mortality, Length of Survival, and Change* In Weight of Animals ixpoeed to tho Vapor of iydraul P-9 in Air. 2. Tho Mortality, length of . urvlval, and Changes in Weight of Animals xpoaod to tho Vapor of Aroclor 1248 In Air. 3. Tho 'ortality, Length of furvlval, and Charges In Weight of Animals cxpoeod to the Vapor of friorosyl Phosphate in Air, 4. Concentrations of Various risooTspositlon Products in Air of Chamber in the several jtperlj.ients. i>. Tho Mortality, Length of Survival, and Changes in Weight of Animals exposed to Aeroeols Formed by Dropping Pydraul F-9 Into an Inconel fube Coated to a Toinporature of 12i>0 P. (677 0.). Ci, rho Mortality, Length of Survival, and Ohangee in Weight of Animals Lxpoaod to Aerosols iForod by Dropping Pydraul F-9 Into an Inconel lube heated to a forboreture of 1050 y, (>66 c,). 7. The Mortality, Length of .Survival, and Chargee in weight of Animals imposed to Aerosols f ormed by Dropping Aroolor 124" Into an Inconel lube oatod to a Temperature of 12 0 P. (677 C.). 0. Hie Mortality, Length of Survival, and Changes in Weight of Animals .xpcfiod to Aerosols iomod by Dropping Aroolor 1?48 Into an Inconel Tube floated to a foirperoture of 1050 F. (306 0.). 9. The 'ortality, Length of Survival, and Chang.ee in '..'eight of Animals :Xposed to Aerosols formed by Dropping Trlcroeyl Phosphate Into ar. Inconel fube Coated to a Tamperature of 120 F {677 C.), 10. The Mortality, Length of .''urvlval, and Changes in '..bight of Animals .exposed to Aerosols i ormed by Cropping: frioroayl Phonphate Into an Inoonol 'Tube Heated to a Temperature of 1050 F, (66 C). TOXSTUDIES0102 j?PNF/DfcNy,A i -2 H. sumary of Mortality teiong Croupe of Antonie Svbjeotod to Aerosols formed by Dropping i'ydraul F-9, Aroolor 1248 or Trlorosyl phosphate into a Heated Inoonel Tube. 12, Incidence of Mortality In Relation to Material and Specie* liegardlesfl of the fer^;oralure of the Inoonel, 13, Inoldonoe of Mortality in Kelatlon to material, species, and Temperature of Inoonel. ll|., Ihe Toxicity of pydraul f-9 When Maintained Continuously for Twenty-four hours In Contaofc Kith the Skin of Rabbits by the f-leave Mottiod of DralBe, Woodard and dalvery. 15. The Toxiolty of Aroolor 1246 When Maintained Continuously for Twenty-four hours In Ccnteot with the Akin of Rabbits by the Sleeve Method of .raise, '.'owlan! and Colvory, 16. The Toxiolty of Trloroayl phosphate When Maintained Continuously for Twonty-four "'ours In Gontaot With the Skin of Rabbits by the Alcove Method of Draiae, Woodard and Calvery. 17. The Toxiolty of Gargoyle DTTS When Maintained Continuously for Twenty-four Hour* in Contaot with the Skin of Rabbits by the .Hoove iothod of ..raise, Woodard and Calvery. . 10. Bustuarlsed Data on the liaeedlate Toxiolty of Pydraul i'-9, Aroolor 1248, TrloroByl i'hospbate and Gargoyle !)T!i Whan ninlalr.ed Upon the . kin of rabbits Aoeordlng to tlie Sleeve Method of braise, Woodard and Calvary. 1 j I : j ! I j | i ! i j ! TOXSTUDIESOI03 CONFIDENTIAL 1 Doated Well From Whloh Pydraul F-9 tfaa Evaporated Into Air Stream*. 2. Tower* From Whioh Liquid# are Volatlllssod. 3. Equipment for tho Donoration of the Doooiqpositlon i roduot# or llydraulio Fluid# at High Tomporatura*. If. Absorption Train lor Oollootlon of Carbon ilonoxlde From Air. 5. Standardisation Curve for th Determination of fydraul. , 6. Standardisation Curvo for tho Determination of Aroolor 12lf8. 7 Standardisation Curvo for tho Determination of Trioreeyl Phosphate. 5. Standardisation Curve lor the Dotermination of Hodlw'f Chloride. V. Standardisation Curve for tho Determination of Dota-Creftol. 10. Standardisation Curvo for the Determination of l'nraCroeol. 11. Schematic Arrangement of Equipment shown In Figure 3. 0615625 TOXSTUDIESOI04 The Toxlolty of the Fogs Formed by Dropping Pydraul F-9. Aroolor 124# and Trloresyl Phosphate. Upon the Surface of a Heated Inoonel (Projeot 49) Soope The purposes of this Investigation were to determine the Immediate toxlolty of Pydraul F-9 and two other hydreullo fluids, Aroolor 124# and trloresyl phosphate, (1) when vaporized at moderately elevated temperatures and (2) when inhaled, t>y animals of several speoies, os fogs formed by dropping them upon Inoonel, maintained at temperatures of approximately 1250* and 1050' F. (677* and 566 0,), these temperatures being, respectively, somowhat above nnd somewhat below the fire point. A supplementary statement oonoernlng the immediate toxlolty of these materials when maintained In oontaot with the skin of rabbits la included at the end of this report. 1. Animals of several speoies survived without signs of intoxioation whan exposed to air laden with Fydraul F-9, Aroolor 124# or trloresyl phosphate for 7 hours per TOXSTUDIESOI05 -2- CON F'E NTiAl day on each of eovoral days, i'he concentrations of tha materials la the air end the numbers of daye of exposure vara as followsi iydraul F-9 3.0 mlorofirama por liter, 1? days; Aroolor 1240, 43.1 mioroirama tar liter, 4 days; nnd trlorocyl phosphate, 01.*! mloroyra.-is per liter, C days. ?. id inhalation of vapors of iydraul 1-9, Aroclof 1f44 snd irlorosyl puoaphaie fenced at moderately olovated room t ..peratures Induced minor obanryae in tha lwr. s of i w animals, .-.espiratery exposure to el liber Fydrsul --9 fir trlorosyl phosphate induced wodorate Inoroasoe In she mi-bore of histiocytes within the lo.ni.; peronchy a and the filr parse ef:. of cr.ts, while correspond lrp, exposure of other species to either i ydraul f-9 or Aroolor 1240 induood interstitial pneumonitis end apparently contributed to the severity of this lesion ns it is observed, not Infrequently, In control animals* Intereurrent infection, unrelated to the experimental proeedura, obscured Interpretation of the morbid changes in tlie tissues of a ; uinoa pip arid 2 rabbits etposod to the vapor of triorosyl phosphate, one of v/hloh tiled 4 days after Che last poriod of exposure. i'he vlceera of tha rats and nice were normal, babbit l'907, the only animal of the yroup exposed to Aroolor 1248 from which tissues wore subjooted to microscopio examination, had fatty degeneration and neorosi# of the .. 0615627 j t ! J i i j j | I i TOXSTUDIESOI06 Jb l!)f -3 hopatio parenohyma. Other animals that were exposed to vapors from Pydraul F-9, Aroolor 1243 or trioresyl phos phate exhibited no hletologlo alterations of the visoera, aside from the lung, whioh oould he aseribed to the toxlo notion of the materials under investigation. J. The fogs of these materials arising from oontaot with Inoonel at either 1250 or 1050* F. were Immediately irritating to the muoous membranes of animals. Kxposure for 20 minutes to the fog of Aroolor 1243 lnduoed labored respiration, but in the oase of the other materials this beoane evident only after exposure for almost an hour. 4. All animals (survived when exposed for 15 or 20 minutes to the fogs formed from Pydraul F-9 or trioresyl phosphate, at either I0500 or 1250* F., but exposure for corresponding periods of time to the fog from Aroolor 1243 resulted fatally. In general, guinea pigs and mloe died more promptly than rats and rabbits. 5. Guinea pigs and mioe suffered greater over-all mortality than did rats and rabbits when exposed to the fogs formed from any one of the three materials. Aroolor 1243 was more toxlo than either trioresyl phosphate or Pydraul F-9 irrespective of the species of animals by which toxioity was tested. 6. The fog of Aroolor 1243 formed by oontaot with Inoonel at 1250* F. was more toxlo than that formed when 0CL5&28 I TOXSTUDIES0107 Co Np/ - If the temperature v&i 1050 P. Tha fog of fydreul P-9 appeared to be slightly more toxic Mixon generated at the higher temperature, but the converse vac true of fogs from trloreayl phosphate. 7. halving the rote of delivery of the liquid upon the heated metal tendod to lesson the mortality induced by inhalation of a resulting fog, but this uae not true In all lnatanoea. 0. 'Ucroaoopio examination of tlnsuea from animals that dlod as a result of exposure to the fogs of i'ydraul f'-9, Aroolor lKIfS and triorosyl phosphate revealed pulmo nary lea tons characterised by intra-alveolar oderoa arid .'.unorrhcgo, diffuse isyporesnia, emphyeewn and extensive deposition of carbon In the bronohl. there was also parenchyma.toua degeneration of the liver, kidney and brain, flaeuea from the animals that survived revealed only pulonary lesions manifested by interstitial pnoulonitlo and Infiltration of oarbon-laden hlstlooytee l,hrouf.bout the alveoli faui bronchi, fho lunge of animals that wore exposed for the shortest periods wore normal, as wore those killed KB days or longer after the last ' period of exposure. 9. The minimum lethal dote of undiluted fydraul )'-9, when maintained In content with the intent akin of rabbits aooordlng to the Klg-hour eleevo method of Dralse, :i TOXSTUDIESOI08 Woodard and Calvary, was greater than 3-*> ml. per kg. and less than 6.0 ml. per kg., of body weight. The oorresponding value for trloreeyl phosphate was between 1.6 and 2.5 ml. per kg. Aroolor 1246 was not lethal In the dosage of 9.4 ml. per kg. Pydraul F-9 and Aroolor 1246 are more toxio if applied to the abraded skin. Materials Pydraul ?-? Is a dear, light amber, oily liquid vihioh has a not unpleasant sweet odor. Its viooosity at 100 y, and 210" F., respeotively, la 56.0 and 6.0 Oentlstokasi its speolfio gravity at 25s/25* 0. is 1.266, its pour point +5 F. Trioresyl phosphate Is a colorless, odorless liquid, the visooalty of whloh at 0" and 25" C., respeotively, is 1256.14 and 66.6 Centletokes. Its moleoular weight is 366.4, its speolfio gravity at 25"/25" 0., 1.162, its flash point 470 F. (43 C.), its crystallization point la less than -35 0., and its boiling point 420' C. The sample used was made from oresyllo aold in whloh there was loss than 3 per cent of the ortho laomer. Aroolor 1246 is a straw-oolored liquid which has a slightly sweet odor. It is more vieoous than trioresyl phosphate. Aroolor 1246 is biphenyl ohlorinated to the TOXSTUDIESOI09 Co Nfr >os NTf 6 *L extent of 1;6 por cent. Its molecular weight Is 192, end lta density Is 1.44 B per wl. at 30 C. (G6 F.} end 1.19 E* pe* wl. at 300 0. (572 F.). Its viscosity ranges from 112 eentlpoises at 30 0. to 0.47 at 300 C., and It distills between 340 and 375 C., Its vapor pres sure being 0.00037 *., 0,16 2.9 m. end 36O.O , of mercury at 37.0 C., 100 0., 10 0. and 300 C., respec tively, eooordlng to HoArdlo, Herrott and benlenus (Ind. ,i\g. Chen. 4111341. 1949). These authors Have quotod the national Hoard of Pipe Underwriters to the' effcot that "decomposition of the produot (Aroolor 124-9) was not appreciable at temperatures below 400 C. (752 F.) but booeme Increasing/ epperent at higher temperatures." The 00-pound Has a flash point of 193 to 196 C., but no flro point in the Cleveland open cup. /hen olthor lydrnul F-9 or Aroolor 1242 was dropped Into an inoonel tube maintained at approximately 1250 F. (677 C.) the liquid burned momentarily with a bright yollow-white or orange-white flame, and then burst into a dense cloud of black smoke. Muoh glossy oarbon was dopoeltod on the sides of the manifold loading to the obamber, which rapidly became filled with dense grey smoke. This-soon turned jot blaoki apparently beoause of the formation of flakes of carbon as soot. At 10i>0 F. (66 C.), these liquids e.rouldered and burst Into a 0615631 TOXSTUDIESO110 Co N fi ll) 7 r'A dendo cToy-white fog. In which were email amounts of car bon soot. In all Instances the odor v.es acrid and pungent. :bon dropped Into the Inconel tube heated to either 1250 or 1050 F. tricresyl phosphate v.avo rise to a charaoter- lstlo phenolio or orosyllo odor end behaved as did the other two storlnln whon droppod upon metnl at t' o higher to'.'porKt''ro. Ait temperatures below 102$ F., trlerosyl phosphate appeared to s-.eouldor without flaming. dxpet'lmental "athods ' pqulpnont. Ida animals wore oonfinod In a ?K3-lltor e.-Kmlrloel stool cho.ibor coated on the Inside surface with a oahod pins tie. Jnless a heavy fog was present the V,o. avlor of tho koI. ,als could be observed through a sheet of cl oar pliable plastic me 'brans, which sealed the otherwise open front end of the chamber. An Interposed heavy v,-ire-mesh screen prevented the snivels from breaking the plan tic membrane, foe contaminated air entered the char .Lor near the toy of the roar wall on which was mounted a circulating fan. Affluent air wee withdrawn throufJtt an outlet located towards the front of the lower part of the chamber by maintaining: a slight negative pressure. Volatilisation of the 'Materials Without Decomposition. i'or the volatilisation of either trioresyl yhospliate or Aroolor 1?1|S, two flet-bottomod cylindrioal bubbling towers 061563? TOXSTUDIESO111 CONFIDENTIAL 8 (2.94" 3t 13") in parallel (Figure 2) were alloyed, a irittod glass thimble (0,94-* * 3.75") being Immersed in the liquid eontelned In eeoh. These towere were kept In a bath or Jtujol maintained at 109 0. Air passed through the two bubbling tower# at rate* of 5.0 and 6.3 lltore per '"inute, I'afpootively, ae measured by a rotamotor in oaoh line, the strenma sorting in a glaae tube leading to a manifold imwraod In a tooling bath before entering the ebanber. At the etart of the experiment S>J>0 nlllilitara of either trlcreayl phoaphata or Aroolor 1248 were plaood In each of the two bubbling touere. In tho oaso of tri- orosyl phosphate# It was found that the concentration of vapor within the ohamber became lea* on th aeoond day of exposure and still lose on the third. It Is thought that the trlcreayl phosphate mi;,ht have hydrolysed some what during the oourae of standing overnight, poaalbiy from raoiature absorbed from the air passing through it during the day. In order to a Intain the concentrations as uniform aa possible, the material remaining In the towera was roplaood with a freah supply on tha fourth day of tho experiment. Uad sufficient material been on hand, this would havo been done daily in order to attain a somewhat higher average concentration throughout an exper iment. This dlffioulty did not arise in the case of tha Aroolor 1248* .. 0615633 i TOXSTUDIESO112 . -9- PPNFJDS^rUl, It was necessary to employ other equipment for the volatilization of Pydraul F-9 beaause of extensive foaming In the bubblers. Therefore, the Pydraul P-9 was maintained at temperatures ranging from 109* to 11* 0. in a glass well heated by niohrome wire (Figure 1). Air at the rate of SO liters per minute, as measured by a rotameter, passed over the surfaoe of the heated liquid and then through a manifold cooling bath before entering the ohamber. A volume of 190 ml. of fresh Pydraul P-9 was addod to the well dally, of whloh about 1J0 ml. remained at the end of the day. Generation of a Fog Prom a Heated Inoonel Surfaoe. A rotamoter for measuring the flow of air, a furnaoe for volatilizing and decomposing the liquid, a seml-potentlomoter (see below), a ooollng manifold, and the ohamber for exposing the anlmalo are Bhown In Figure 3. In those experiments the smaller rotameter, to the right in the photograph, was not employed, and all air (0 liters per minute) that entered the chamber was passed through the furnaoe. The ooollng colls were paoked in the bath shown and surrounded by orushed lee. The body of the furnaoe oonsisted of a 26-lnoh length of oold-drawn, seamless Inoonel tubing, having an outside diameter of 1.5 Inches and a wall-thickness of 0.0*19 lnoh. Pydraul P-9, trloresyl phosphate or Aroolor 12*1(1 TOXSTUDIESO113 - 10 - C O N F I.D 5 N T 1 A l j i was droppod upon the lnnor surfaoe of the tube from a burette through an Inconel tube weldod to the uppor surfaoe of the heated tube near Ita midpoint, This side tube had a length of 6 Inches, an outside diameter of 0.675 inch., and a wall-thickness of 0.091 inch. Another plooo of Inoouel tubing J Inohae in length and having an outnldo diameter of 0.436 inch and a wall-thickness of 0.049 inch, was also weldod to the upper portion of the furnace tube, to permit the insertion of a thermocouple in such a manner ae to measure tie temperature at the upper Interior surface of thefurnaoe. The distance from cantor to center of the two smaller tubes, which wore parallel to one another and perpendicular to the furnace proper, was 1,75 Indies. The outside of the furnace wan wrapped with 2 layers of molding raloa. roe middle 10-inoh portion of the len, th of the furnace was elootrlcolly heated by means of 20 feet (9 wrappings to the inch) of A. w, b. 'uvbor 20 Tophet "C" wire, which has a resistance of 0,659 ohras per foot at 60 !'. 1'he wire uaa insulated with approxi mately 0.75 inoh of diatonaoeoua earth and wrapped on the outside with asbestos doth. The temperature of the wall was meecured by an lronconstnntan thermocouple attached to a semi-potentiometer calibrated against a student potentiometer. The thermo couple waa in oontaot with the upper part of the furnaoe ; . i j ) i j ; I : 0615&35 TOXSTUDIES0114 *-^rvr.v CONFIDENTIAL ' - 11 about 1 inoh downstream from the point at whioh the droplets of material fell upon Its lower Interior surfaoe. After the furoaoe had been brought to the desired temperature of either lOJO* F. or 1250* F., the hydraulic fluid was dropped from a burette at the rate of 0,067 to 0.245 ml* per minute (of. Table 4), 0,100 to O.I67 ml, per minute being the usual rate. Sampling and Analysis of Contaminated Air. When the undecomposed materials were metered Into the air in the ohamber, 4 samples of air eaoh day were passed from the chamber, at the rate of 3.4 liters per minute, through 2 absorption towers (2.25" * 13" outside diameter), eaoh containing 100 ml. of an appropriate solvent. Redistilled ethyl aloohol was used In oolleotlng trloresyl phosphate or Aroolor 1248, while hexane was used for Pydraul F-9. The sample passed through a ooarse fritted glass dlso (1.13" In diameter) Immersed In the solvent and plaoed parallel to the bottom of the tower and about 0.2" above It. The oontent of Pydraul F-9, Aroolor 1248 or trloresyl phosphate so colleoted was determined by measuring, by means of a Beokman spectrophotometer, the ability of the solution to transmit ultraviolet radiations of wave lengths 246 mg, 246 nig. and 264 nog, respectively. 0615636 TOXSTUDIESO115 r A ourve relating the transmission at 246 njiO-to the concentration of Pydraul F9 In hexane over the range of 4.7 to 71 mlorograma per ml. Is shown In Figure 5* A comparison of the corresponding curves for Aroolor (Figure 6) and trloresyl phosphate (Figure 7)> are also shown. The alcohol used as solvent for Aroolor and trlcrasyl phosphate vies freed from ultraviolet-absorbing impurities by the following procedure! About 175 ml. of 95 per cent ethyl alcohol were permitted to stand overnight with 10 g. of pellets of KOH and 7 S< aluminum foil (0.003"). The mixture was then refluxed for half an hour and distilled at c*< the first 20 ml. being rejected. In experimente Involving the exposure of animals to the produots formed on thermal decomposition of rydraul, Aroolor or trloresyl phosphate for no longer than J hours, from 3 to 8 samples of air v/ere collected from the chamber for analysis, depending upon the length of the period of exposure. When exposure was repeated on the following day, a corresponding number of samples was collected on tha seoond day. Analyses were made for one or more of tho following materials! phosgene, hydrochloric acid, free oresols and oarbon monoxide. 061563? TOXSTUDIESO116 - 13 - .CONFIDENTIAL ! When Arcelor 1248 was to bo determined, the oarriplee i ; of tiir were collected In an avaouated glass bulb 21.455 I liter* In capacity. The Aroolor In the cample wee ] absorbed in 100 ml. of redistilled ethyl alcohol, the 1 latter be Inf. released Into the bulb from on auxiliary bulb by opening an Interposed stopcock. In the I Mrat few analyses. It was found necessary to free the alcoholic ; j solutions from carbon soot by filtration, but subsequently trie noed for ro doing was eliminated by the insertion of a plug of ; ls.as wool between the dumber, and apparatus for colleotlon. Suitable aliquot portions of the alco- hollo solution were o-ployed in the analyses, When phosgene was to be determined, the air withdrawn iron the chamber wan passed at the rate of 6.(5 liters per minute first throw h u column 1.5" x 0.5" or 60 to CO icah sine dust, next through a fritted , lasa dlso lm ersed in 100 ml. of s 4 por cent aqueous solution of sodium hydroxide contained in a cylindrical tower 13" in height and 2.25" in outside diameter, and lastly through an i.splnger similarly in-ora ad in a like volume of alkaline solution In a second tower of the same dimensions. "amploo of air to be analysed for hydroehlorio sold were J collected similarly oxoept for the omission of the column j of slno dust (of. Jacobs, Analytical Chemistry of industrial Folsons Hcaards and Solvents, p. 305, 306, I I : 0615636 TOXSTUDIES0117 .CONFIDENTIAL j 14 1941). It ter n aliquot portion (15 ml. or lost) ol' the roluttun :nd been r-ade sold to phonolphthaleln with 10 per eent. nltrlo sold (1 vol. of iiflOj 3.0. 1*42 diluted to 7 volumes with water), and diluted to ?5.0 -;1. In a volumetric finale, 3 drops of 0.1 K sliver nitrate wore ridded. rue tronrmissl.on of 11; ht of wave lurubh 500 ryi firou1 h tills turbid solution In a >0 rtri. coll was ueasurod in s ccV.arn spectrophotometer In equip ru'lv on with I.' at through a al".liar ocll containing o ohlorlcle-frco 1olution prepared in a corresponding -icnnor. A ourvo relating the transmission at 500 nj* to the concentration of sodium ehlorldo over the range of 20 to 320 nlorourans per ?5 rl. la shown in f-l,c.ura 0. One mllllprara of sodium chloride la equivalent to 0.h!j61 ru'. of phosgene or to 0.6233 iisi. of hydrochloric acid. i':e erosola were determined colorImotrlcelly nl'tor co*p tin with diazotlzod sulfanillo acid by icthods for the meta Isomer described by Koanot and henan (Anal, chom. 201951, 1948)< and for the para Isomer by Schmidt (J, idol. Cham. 1791211. 1949). famplee of air for the determination of either trloresyl phosphate or free * orosola {*;0In and para icomors) were passed at the rote of 6,8 liters per minute for 30 to 45 minutes through a frltted-class disc scrubber and Impingor In series as in the oase of phosgene. In this Instance, eaoh tower ! | 1 1 i _ ; 0615639 TOXSTUDIESO118 - 15 - PONFIDENTIA L oont.fiinod 100 i'll, of t 1 por cent podium hydroxide solu tion. In enelyilng for ttie free orosols, 1 si. of a 20 por cent sodlua carbonate solution was added to an aliquot of 6 ml. or lee# contalnod In a 10 nl. class-stoppered graduated oylinder. After the solution had boon diluted to 6 ml., 2 ml. of dlazotised sulfnnlUo acid wore added. The latter was prepared by adding 0.75 ail. of a 5 per oont t elution of sodium nitrate to 0.75 :il. of a solution of rulfanllio acid contained in a 25 sil. volume trio flask previously cooled in an loo-bath. Trio eulfanlllo acid polxition was prepared by dissolving 4.5 o of the acid In 45 nl. of concentrated hydrochloric acid and diluting it to 500 ml, with viator. After the partially diazotlsed solution had been cooled for 5 minutes, an additional 3 -.1, of a odium nitrate solution were added and further cooled (or 15 minutes# then diluted to 25 si. The solu tion was prepared daily and kept cold. Curves role tint' the transmission of light of wove lengths 4?5 ryt and 500 tyt to the concentration of .vote ereeol over the ran; of 3 to 49 mioroKNune per 100 ml. are ahovm in ii; ura 9. In making the necessary measurement#, the oomparlron-oell contained a solution similarly prepared oxoept for Lha omieelon of eresol. Corresponding curve* for pera-oroaol over the range of 10 to 120 miorograrae per 100 ml. are shown In figure 10. Oy the use of the following oeiitao TOXSTUDIESO119 SONFIDfikTIAt 16 ainiultnneoua equation*, the amount of net* end per* creaol could bo calculated. ( For the determination of met* orecol (") i p-oroeol + !| .31 fi-oresol * apparent p-orool (425 ry<) p-eroeol + 1.13 ft-eresol * apparent p-cresol ($00 ryu) 3.IS :t-orasol difference In apparent p-crcool at the 2 wave length* (1) :'-oroeol dlfferenoe In apparent p-creaol " T.18~ or the determination of para eresol (t)i h-craaol + 1__ _ p-orofiol apparent H-cresol (422 ^) 4.13 Vcrorol + 1__ _ p-oreaol * apparent :!-orosol (500 rju) 1.13 0.653 p-oreeol * dlfferanoo In apparent ;i-oroaol at the ? uuve length* (Y) p-oroeol a difference In apparent `"Croiol ~ 0.653 i'iie validity of tills: method for the slmltanooue determination of the too isionern was* demonstrated by the following determinations on samples containing both of thorn In known amoimta. Analysis of a sir "pie prepared to contain 12.30Y of rrt-crosol and 9.9621 of p-oresol yielded 13.60 nd 9.9SY, respectively. A second sample preparod to contain 24.601 of m-oresol end 9.96Y of the par* laoraer yielded 25.901 and 10.TY, respectively. fho recults designated as froe eresola In Table 4 reproeent the sum of the two lsomera. From 60 to yo per 061S6S1 TOXSTUDIES0120 - 17 - CONFIDENTIAL sent of tho total amount of free eresola present In the ohamber, aa tha reault of decomposition at 1050 F.i vaa found to oonalat of tha mote. l*omr, but when tha tem perature of tha fumaoo was 1250 F., only tha raota isoier waa proaent. Since a cample of trlcresyl phoapliata, analysed after hydrolysis, contained approximately 6*> par cent of the >sota and 35 par eont of the para isomer, it appear# likely that the latter underwent decomposition to unidentified fragments at the higher temporeture. For the determination of undeeorapoed trlcresyl phos phate proaent in the chamber, a nl aliquot portion of the sample, eolleoted in the manner indicated for the determination of cresols, wo# hydrolysed by refluxing it for an hour with 10 ml. of 45 per cent aqueous sodium hydroxide) It was them acidified with concentrated hydro chloric acid and distilled until 300 ml. had been oolleotod. The usual oroBol method was then applied to the distillate. Carbon monoxide was determined by a nodIficetion of the mothod of Bland (dust. J, xper, Biol* and 'fed, Pol. I0t35, 191)0), The sample* were eollooted by passing air at the rate of 4.7 liters per minute for $ minute# through the abaorption train shown in Figure 4 by moan# of a cast Rotary Blast and huetIon Pump located beneath the table and not show* In the photograph. The first il-tube con tained glass bead# and a 10 per cent aqueoua solution of 0615642 TOXSTUDIESOI21 confidential - 16 - load aeotata, while the seoond 3-tube, which contained sulfuric acid (S ,rl, l.Oi;) and glass beads, was aurmounted by a Ion.-, narrow tube packed from top to bottom with cuoceaBive Isyore of allioa fol {1 j-rognoted with a. Cobalt salt to Indioete the preeonoe of noleture), noslum parclilorntc (Anhydrone), a odium hydrate aabontee oborbent (Aacarlte) and a eoond layer ol' ftyneeium perchlorate, itir issuing from too top of this tuba parsed through a tube containing iodine pentoxide heated to lib0 0. Codine liberated by the resotion 0 --- bCOg Xg woe collected In n bubbler containing 5 ml. of a 3 per cant aqueous solution of potassium iodide. The rate of flow was ioiiiurod by an upright manometer as shown in fInure lj., the contents of the bubbler wore titrated with 0,001 H araonlous acid, alloying starch ea indicator, f lg i UgO --"t fjAbOj^ * i- cI One milliliter of 0.001 N araenloue add *c* 0.070025 nt(5. of 00. In calculating the results, allowance was made for tho previously determined trace# of free Iodine In the volume of the solution of potassium iodide present in the bubbler. bach of the following oonoentrstions of tho various contaminants of the air of the ohsraber, expressed as OfclbbSi p.p.ra, by volume In mi# at Blp0 F. and 745 mm. of mercury. Is equivalent to the Concentration of 1 ng. per liter. i.gis.tAi.Jg-j'jtE.taA I'hoesone 55.9 HOI Trlorosyl phoaphata 693-3 6G.6 Ci'orol 233.8 Carbon monoxide 902.7 Arcelor 124-6 131.7 Xeerlmontal ,'oauite A croup of animals. consisting of a oat, 2 guinea pipe. 5 mice, 2 rabbit* and 3 rata, survived when exposed to air laden with 3.8 mloro..rtraa of iydraul F9 per liter for 7 hour* on eaoh of 5 consooutive days (Table 1). All animals of a croup slr.llarly oonetltuted, oxoopt for the omission of one rat, eurvlvod when exposed to air containing 4-3.1 mlorograms of Aroolor 1248 for 7 hours on eaoh of 4 oonseoutlve days (Table 2). ixoopt for one rabbit (F798) that died from oausee unrolatsd to oxposurs 4 days after the 8th exposure, those of a 3rd group eurvlvod when Bubjootod to the Inhalation of air bearing 61.5 mlorograme of trioresyl phosphate for 7 hours on oeoh of 8 days over a period of O&lSbAA i i TOXSTUDIESOI23 CONFIDENTIAL 20 10 day* (Table 3) Changes In weight were usually email (Tablet 1 - 3), and no alga* of Intoxication wera observed. Miarosoople examination of the lunge from animals exposed to Pydreul F-9, and killed 10 or 13 days after the 5th period of exposure, revealed the oaourrenee of Interstitial pneumonitis In the ret, mouse, guinea pig and rabbit, rtie oat exhibited moderate numbers of hls- tlooytos, containing dark-brown granular pigment, within respiratory bronchioles end alveolar duots. The remaining viscera were normal. , Animals exposed to the vapor of Aroolor 1P'48 nlao had interstitial pneumonitis, l'he lung* of a ost whloh had boon subjected to exposure to the vapor of Aroolor irt|B wera normal. There was no evidence of Injury to the other viscera of the various species. The liver of a rabbit (1907), the only one examined mlerosooploally, exhibited severe fatty degeneration and fool of necrosis. 'leroeoopio examination of tissues from a rabbit (b'798) that died 4 days after the 8th exposure to tri- oroeyl phosphate dieolosed the presence of a severe paraaitlo infestation in the liver. Another rabbit that was killed 3 days after the 8th period of exposure had inourred severe parasitic infestation of the liver, A guinea pig had acute lobular pneumonia, unrelated to the experiment. In the lunge of a eat were considerable 06156*$ TOXSTUDIESOI24 - 21 - CONFIDENTIAL numbers of hlstlooytes, containing dark-brown granule*. The viscera of the rate and mloe vor* normal. Exposure to Arcolor 1240. or 4h>r in C.mtaot wAtfr Inaon^ at 1250 or 1050 F. The duration of exposure, the changes In weight, and the fate of Individual animals that were aub Jeotod to the Inhalation of the producta nrloins from the contact of lydraul f'-9 with Inoonel at 1250 and 1050 F. are proeentod In `fables 5 and 6, respectively. Corresponding data from animals exposed to the fog* from Aroolor 1248 or trloresyl phosphate are given, respectively, in fables 7 and 8 and In fables 9 end 10, i'he data relevant to mortality among all groups have boon summarised In fable 11. Mortality. Careful examination of the data domon- atrato certain . oneral facta, It la apparent, in terms of over-all mortality, that ninoa pigs and mice wort ,, more nuseoptlble to each of the throe materials than wart rate and rabbits (1'ablo 12). Tho same oritevlon was employed for comparison of the fogs that were formed at 12o and 1050 F. '-'he fog generated from Aroolor 1248 at 1250 F, was more toxlo than that formed at 100 (fable 13). In the case of fydraul F-9, the toxicity of the fog formed at the higher temperature appeared to be slightly greater then that 061^6`ib TOXSTUDIES0125 . * 22 jSP.NFIDCNT/At, ....... generated at the lower. The temperature of dooosiposition ua of little Importance In determining the toxicity of the fog formed from tploroayl phosphate, slrsoo that which rosuited from th higher temperature wan apparently lightly leaa toxio than that formed at the lower temper ature. A conpiu'iron of th throe Material* under corresponding oondltlona lndloatea that the fogs formed from Aroolor 1240 were more toxio for guinea pig, rabbita and nice, but not for rata, than were thoee formed from Pydraul : -i or trioresyl phoaphat (Table 12). Aroolor 1248 i clao the moat toxio of the three materials If the ahorteat period of exposure that is lethal is taken aa the ori.orlon. 1:osardies a of th temperature of decomposition, all animal survived when subjected to the fog formed by l'ydraul i-'--9 or trloresyl phosphate for 1> or 20 minutes, but exposure of like duration to the fog oroated by th thermal decomposition of Aroolor 1248 often resulted fatally. In order to ascertain the effeot of different rat* of delivery of the liquid Into the Xnoonel tube while the rate of flow of air through the tube was kept con stant, Aroolor 1248 and trlcresyl phoaphato were delivered at two different ratea (Tables 4 and 11). The mortality esaoolsted with reduotlon of tire rate of delivery was relatively low In many instonsea. 06156*1 7 TOXSTUDIESOI26 . - 23 - fONFlDCNTIAh i'.jf-ns and Pathology of Intoxication. Although In the** experiments th fogs filled the chamber to auoh an extent that It was Impossible to observe tho animals throughout tlie period of oxposura, a l'ew signs of lotox'cation could bo observed* The fogs wore immediately Irritating to tho mucous membranes of tho wiinals, as ovldenoed by tholr oloaure of tho eyes, twitolling of mo nose, orylng, coughing and aneeslng. labored respiration and Irritation of tho superficial tissues of tho oyes appeared later. Dyspnea was observed after 0 minutes of exposure to Aroolor 1?4> but this did not appear until the animals had been exposed to the other atorlala for almost an hour, henovor cats ware exposed exooaelve salivation was evident, and frequently long strands of thtok nuous drooled from tho mouth, Made soot oovored tho hair of animals exposed to fogs generated at tho higher temperature (1250 F,). examination of anlmala that died during or chortly after exposure to the fogs of pydraul : -i, Aroclor 121(8 or trlorouyl phosphate revealed extensive pulmonary congestlon and, In occasional animals, foci of pulmonary hemorrhage. ''any also had foci of black pigment tliat appeared to bo oarbon distributed throughout the lung tissue, -llorosoopioally, the lesions consisted of narked hyperemia of tho lung, common to all expoeed anlmala 06156^8 i j j j I i : TOXSTUDIESOI27 - 24 .0 N PlQ *NTi U examined, end in many thora vm lntre-alveolar hemorrhage and edecia. The oarbon pigment wee dletributed ohlofly within the bronchi end, in some lnatanoo*, completely odbluded their lumen*. Pulmonary emphysema we* preont frequently. In 5 guinea pig* end 2 rebbite that died during exposure there was, in addition, an acute purulent bronohltle, apparently a reaotion to bronohlal irritation. Lot'nally poisoned animal* exhibited parenohjmatoua degenoration of the liver, kidney and brain with regularity, in addition to thd, pulmonary lesion*. The vieoera of animal* that died ^ to SO day# after brief periods of exposure revealed pulmonary lesion* similar to those already described ex#*pt that there'itra* more phatoeytoaie of oarbon partial*#* diiitSai*. that survived were killed after intervale varying frord 1 Week to 2 month*, There "were no significant lesion* of vieoera other than lung. Two oate of tire 5 exposed to the fog of Pydraul i'-9 wore examined mioroaooploully. The visoere of Oat A38l wore normal, while the lungs of Oat A374 were the site of pro fuse intraluminal bronchial deposit* of blaok granular material and interstitial pnoumonitie. The lung of Cat A3?l, whioh had been subjected to 2 period* of exposure to the fog of tricresyl phosphate, was characterised by similar pulidonary change*. Guinea pig A862, jellied 22 day* after 2 periods of exposure to triore*j|V phosphate otase'i? ) 4 k TOXSTUDIESOI28 c^f 25 -- M t vaporized at 1850* F,, was found to have numerous hlatiooytes loaded -with oarbon pigment in the lungs, as well as a severe interstitial pneumonitis. Other speeies of animals had a similar interstitial pneumonitis. However, animals that had suffered the shortest periods of exposure, and animals hilled t days or longer after exposure, exhibited normal viscera. The Concentrations of Identified Decomposition Prod ucts Formed at the Higher and Lower Temperatures. Although only a part of the materials metered Into the Xnoonel tube could he nooounted for in the air In the ohamber, some very tentative statements oonoernlng the composition of the air may be made. Although large amounte of soot were formed, no attempts were made to measure the amount of free oarbon or of oarbon dioxide, (lore unidentified materials were present when the deoompositlon was effeoted at the higher temperature, irrespeotive of the nature of the liquid dropped into the tube. In the case of Pydraul F-9, less hydroohlorlo sold and more free oresols were present when the temperature of oontaot was J.0500 F. than when it was 1250' F. (Table. 4). When the temperature of oontaot with Pydraul was 1050* F., the concentrations of hydroohlorlo aoid and phoagene, measured together in terms of hydroohlorlo aoid, ranged from 0.35 to 0.67 p.p.m., but when the temperature ObV5f>50 TOXSTUDIESOI29 - 26 - '/p '0 rh was 1250 7. the corresponding values ranged from 11 to 22 p.p.m. Free oresols were more abundant at the lower temperature, being present to the extent of 11 or 12 p.p.m., whereas only 0.09 to 0.15 p.p.m. were found v/hen the temperature was 1250* 7. In experiments v/lth Aroolor 124(5, nuoh more undecom posed material was found present when the temperature of contact was 1050 7. than when it was 1250 7,, 121 to 2(56 p.rj.m. being found in the former oases and only 10 to 16 p.p.m. in the latter. Only rarely was more than one p.p.m. of phosgene found regardless of the temperature, tho values reported as hydroohlorlo acid and phosgene representing predominantly the former. Less hydroohlorlo cold was present et the lower temperature, there being 11 to 17 p.p.m. in this ease and (54 to 102 p.p.m. when the temperature was 12506 7. The values reported for oarbon monoxide are probably somewhat low beoauae the rotameter employed for the oolleotion of the samples had not been calibrated for use under the conditions of these experi ments. Nevertheless, it seems apparent that when the Inconel was kept at the higher temperature the amounts of oarbon monoxide found tended to inorease as the duration of the experiment inoreasedj muoh less variation wae encountered when the Inconel was kept at 1050" 7. 1 0615651 TOXSTUDIESOI30 - E7 - '^f0 ?/.aL In the case of triereayl phOBphata, more of the undeoompoeed material and more free oresole were present In the chamber when the temperature of the Inoonel was lower* The values reported (Table 4) for trioresyl phos phate, as determined by hydrolysis and the oolorimetrlo determination of the resultant oresols, may be somewhat low, sinoe results several fold higher oould be obtained by measuring the ultraviolet absorption at 864 iyof alooholio solutions of spot samples oolleoted In an evaouated balloon. Determinations by this means may, however, have given high results, sinoe filtration failed to remove a Blight smoky turbidity. 061665? TOXSTUDIESOI31 88 - Co Np, n rMi Diaouaalon It 1# unlikely that any of the fatalities in these experiment# could bo attributed solely to the preser.o* of phoe*n* *lne#, a-coording to l'lury and 2emlk (Harmful bases, -orlln, 1931. p. 228). mice must ba exposed for several hours to the concentration of 1.25 p.p.m. if any are to bo killed, while only 70 per cent of a croup of nice died when exposed for 20 minutes to air bearing 20 p.p.m. On numerous ooeaaiona the mice In -our experiment* died when exposed for but one hour to an atmosphere that contained Ices than 1 p.p.n. of phosgene. I'lury and hornik state also that rabbit* aurvivod following oxposure for 50 minutes to the concentration of 50 p.p.m., but that 75 por cant of another group died when exposed lor twie* that period. Box and Cullumbine (Ur. J. Pharmacol. and Ohon, 2t30, 1947) report that 8 of 12 rata died after being exposed for 10 ninutes to 0.23 mg. of phosgene per liter of air (57.S p.p.n.), although the concentration of O.OGO >;> per liter (20.5 p.p.a.) wa not lethal for rate when inhaled for 10 'minute*, In the concentration of 1.3 to 2.0 mg, por 1. (1174 to 1605 p.p.m.), carbon monoxide caused no significant sign* of lntoxioatlon in alee exposed for 6 hour* by Chorno and Liberman (Farnakol. 1. Tokslkol. ^0 (Mo. 3)122, ' 0615653 ! j 1 , ; ' 4 1 1 i I t I I TOXSTUDIESOI32 CO {0, 29 1947j 0. A. Jjt2679, 194.8), although 0 to 40 per oent of a group of mica died u a result of a like period of exposure to the concentration of 6 me* of oarbon monoxide per 1* (5416 p.p.m.). Since the mioe In our experiment* died under condition* which were much lea* severe with respect to oarbon monoxide, It ie evident that thle com pound by itself was not responsible for their death. Comparable information with respeot to other species of animals 1* not rsadily available, although (foes, Jackson and Selberlloh (Arch. Ind. Hyg. and Toxicol. Jti6, 1951) have reported that the concentration of 5,000 p.p.m. wae required to kill a rat in 30 minutes* Rabbit* snd guinea pic* survived When subjooted to the lnhslatlon of the vapors of hydrogen ehlorlde in the approximate concentration of 50, 700 and 1,000 p.p.m., over the respective period* of 7, 2 and 0.08 hours (Troon et al.. Arch. Ind. Hyg. and Toxicol, 7l6, 1950). In the** same experiments, some of the guinea pig* and rab bit* died whon exposed for 0.5 to 5.0 hour* to concentra tion* of hydroohlorio sold ranging from 1,000 to 400 p.p.m. Again, hydroohlorio sold doss not seem to b* the solo agent responsible for the deaths enoountored in the present experimente. Data pertaining to the toxicity of oresole in air are not available] however, Deichmann, Kitamiller and t r -4 i i i TOXSTUDIESOI33 30 Kltherup (to. J. CUn. fatb. Uti73 1944) found that only after 20 period* of exposure, eaoh of 7 hour*, to the vapor of phenol In the air In the concentration of 0.1 to 0.2 rag. per 1. did any respiratory distress or fatalities develop among a group of 12 guinea pigs* Kabblt# and rat* survived rauoh longer* Since lb la likely that the vapor* of meta- and para-orosol ara comparable In toxloIty, it doe* not appear that the deaths reportsd herein ore attributable to oroaol. 061565% TOXSTUDIESOI34 *1 * 31 It* Application of fydram P-9, Arcelor 1249 snA . In sooordanoe with the tech nique of Praia, Woodard and Calvary (J. fharn. keeper, i'berap. C2i377. 1944), on* of a series of graded dosage* of oaoh of the undiluted material*, Pydraul F-9, Aroolor 1240 end trioresyl phosphate, was maintained for 24 hours In oontaot with the intact skin of on* of a group of rab bits. Xn this method, the hair was ollpped from an area of shin 6 to 7 lnohe* wide and completely anolrollng th* trunk of each rabbit. In other group*, the outormoat layer of skin was abraded by scratching thin furrow* 7.5 on. in length and a few millimeters apart with an hypo dermic needle. Xn either case, th entire trunk was covered with a sleeve of dental rubber dam whioh fitted tightly at the oophalad and oaudad edge* of the ollpped area and loosely over the intervening srea. During the 24-hour period, of oontaot the rabbit was kept In stocks. At the end of the 24 hour*, tho material was removed from the skin of the animal* by washing with water and a sulfonated oil, "pH6". Xn addition to tho three materials mentioned previ ously, a product designed to fulfill th* sane purpose, TOXSTUDIESOI35 ``typ to 32 Gargoyle DTK, waa investigated. Gargoyle DIB (Sooony) la said to bo a patroloum baao hydraulle fluid 0onto1nine minute amount* of * ruat Inhibitor and an oxidation inhibitor. Gargoyle DTE ha* th# speciflo gravity (API) of-Jl.l, a raaxlwua pour point of 20 F., a Minimum flaah point of 39S F.j a fire point of 497 F. It* viscosity at 100 F., 130 F. and 210 F. 1* 2.2$, 116 and 48.5 S.3.U., roapaotlvely. xporlmental Result*. The pertinent data of th* experiment* relating to Fydraul P-9# Aroolor 124S, trl- orosyl phoaphate and Gargoyle DTK are given in Table* 11), 15, 16 and 17, reapootlvely. Table 18 aunmarlte* the essential Information In the preoodlng table*. Two rabbit* died following th* application of 9.4 ml, of Pydraul F-9 por kg, of body weight upon th* lntaot skin of oaoh, uhorea* only 1 of 3 rabbit* dlod when tho dosage was 6.0 ml. per kg. Throe rabbit* sur vived when oaoh wee subjootod to the application of 3.6 ml. per Kg. Tho dosage of 6.0 ml. par Kg. was lethal to 3 rabbit* when applied upon their abraded kln (Table 14). . ' Two rabbit* died following the applioetlon of 6.0 ml. of Aroolor 1249 per kg. of body weight upon abraded akin of eaoh, where** only 1 of 3 rabbit* died when th* Tt ^4 j 0616657 1 ) TOXSTUDIESOI36  * r - 33 - '-'V r, -u dosage waa 9.4 or 9 ml per kg. The dosage of 9.4 ml. per kg. was tolerated by 3 rabbits when applied upon their intaot skin (Table 15)< The application of trloreayl phosphate upon the Intaot skin resulted in the death of a rabbit given 9.^ ml. per kg. and In that of another given 6.0 ml. per kg. When the dose was reduoed to a.5 ml. per kg., 1 of 3 rabbits died, whereas 3 rabbits, eaoh given the dosage of 1.6 ml. per kg., survived (Table 16). Viva rabbits with Intaot skin and 3 rabbltB with abraded akin survived following the applloation of 9.4 ml. of Gargoyle DTK per kg. upon their skin (Table 17). In no lnstanoe was the intaot skin of the rabbits Injured by the applloation of these materials. Nothing remarkable occurred when Gargoyle DTE was applied upon the abraded skin; however, the abraded skin beoame very slightly erythematous after Pydraul F-9 or Aroolor 1248 had been applied, From the Kettering Laboratory In the Department of. Preventive Mediolne and Industrial Health, College of Kediolne, University of Cincinnati, Clnoinnatl, Ohio, Experimental Work and Report by: Joseph F. Treon, Ph. D, Prank P. Cleveland, M. D, Frederlo IS. Shaffer, M, S. John Cappel, B. S. Thomas Gahegan , William Wagner Approved Robert A, Kehoe, M. D. Dlreotor . Date Maroh 11, 1953 TOXSTUDIESOI37 l. Jhe Mortellty, Length of Survival, end Ohanr.ee In Ma.tr.ht of Animate Sxuoncd to the Vapor of fydraul F-9 In Air (Conoontratlon or pydraul i'-9 0.00377 ) (Duration of iJxpoeurei 5 x 7.0 hours) Identlfioatlon Humber Change in '/eight Depressed a* Peroentara of Initial Wolght Initial During After Iveltibt Period of Period of kg. exposure iixposure Tate Lonpth of survival After xpoeure I'-yforo the Anliiela ./ere Killed Day* oat A359 2.504 Guinea Hi; A?lj.O Outr-oa f';: A7l|l <>.909 0.961 2 sice 3 -ice Knbbit li`?k ) AV. ) 0.0198 ) ?,038 babbit F32S Ret DS39 1.963 0.248 Fiat Di,'40 0.209 net DJljl 0.252 -2.4 -1.4 -3.1 * -54 -0.9 +1.2 +3.3 -1.6 + 1.3 - 0.5 '- " 11.8 15.7 3.8 + 1.2 Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived . 10 10 13 10 13 10 10 10 13 13 OfcltAVt TOXSTUDIESOI38 t-yytt-irip- 2fiSi*JL m*. Mortality.. UngmofaurTival. and Change*., In freight (Concentration of Aroolor 1248* 0.01(31 mg./l,) (Duration of Exposurel 4 * 7.0 Hour*) Idontifleatlon Number Change, in Weight Expressed a* Fereentege of Initial Weight Initial During After Weight Period of Period of kg- Exposure Exposure Fate Length ol Survival After Exposure Before ttu Animals were Kill* Dap* Cat *378 4.300 Guinea Fig *757 0.988 Guinea Fig *758 0.939 5 Uoe Av. 0.0178 Hobbit F906 .044 Rabbit F907 Rat L203 Hat E284 .144 0.179 0.170 2.8 - 4.8 - 4.2 +12.4 - 6.4 + 1.7 11.7 +14.7 - 3.0 0 - 2.3 + 6*7 13.1 +11.2 Survived Survived Survived 2 Survived 3 Survived Survived Survived Survived Survived 6 6 6 6 7 6 7 6 7 ----- --------- H 06ibbb0 TOXSTUDIESOI39  i *. T,,T'i ,*JC* *m ,o I ' Tfrt MertgA<3r,.j!^M^ fitofflBM. 1ft .vf.iEM.-e AnlmtlJLKxPoeed to the vapor of Trtereeyl Phoethate .m Air (Concentration of rrloreeyl phoaphatei 0.0615 mg./l., 4*2 p.p.m.) (Duration of Scpoaure 8 x 7*0 Hour*) Chang# In Weight Expressed as Percentage of Initial weight Initial During After Identification Weight Period of Period of Humber Re. Abcpoeure Bxpoaure Fete Length of Survival After Exposure before the silled (K) or Died (D) Daye Oat A353 3.226 Guinea ir'lg A?33 0.889 Guinea He A734 0.811 5 .'lloe Av* 0*0164 Rabbit 1797 Rabbit i'790 1.779 1.841 Rat 1)536 0.130 net l>537 Rat d,'38 0.140 0.129 + 5.6 + 1.6 - 0.5 +13.4 + 4.6 + 6.5 +lJ|,6 + 7.1 +28.7 0 + 4.8 - 0 + 6.2 25.7 " Survived 3 K Survived 3 K Survived 10 K 2 Survived 3 K 3 Survived . 10 K Survived 3 K Survived 4 o (l> Survived 3 K Survived 10 K survived 10 K (1) Death due to par&Bltio invitation of liver unrelated to exposure* 0615661 TOXSTUDIES0140 Table 4 C/,_ Concentrations of Various Decomnosltion Products in Air of Chamber in the Several Experiments . ?/V> Material In Contact With Inconel Variation in Temperature of Inconel ?. Experiment Number Material Added ml./min. Expressed as mg./l. on Upper Line and p.p.su on Loiver Line . Aroclor Phosgene HC1 (uncor rected for Phosgene Trieresyl Free Phosphate Cresols . 'i Cartoon \ Monoxide \ Pydraul 1240 - 1256 1 0.143 - Pydreul 1245 - 1260 2 0.133 - Pydraul 1240 - 1250 3 0.167 - - 0.032s 22.7 - 0.01S0 12.5 - 0.0164 11.4 - 0.00065 0.15 '1 -i - O.OOO52 -4 0.12 1 3 - 0.00036 -! 0.09 4 Pydraul 1245 4 0.245 - pydraul 1245 5 0.220 - Pydraul 1040 - 1055 6 0.132 - - ' 0.0155 10.7 -- - 0.0005 0.35 -- --- - 0.0459 10.7 .* -i 0615662 i TOXSTUDIESOI41 ---------------------- 1-------------------- Material 1b Contact With Inconel variation in Temperature of Inconel ?. Experiment Humber Table {Pas 2) /1a* Expressed as mg./l . on Upper Llne and p.p m. cm loiser Line . Material Added ml./min. Aroclor Phosgene HCl (unoorreoted for Phosgene) Trieresyl Phosphate free Oresols Carbon ! Konaxid. Pydraul 1047 - logs 7 0.125 - Pydraul 1049 s 0.113 - - 0.C012 0.S3 - Q.0W5 - - : U-3 , ' i 0.00096 - 0.0447 0.67 11.4 j Pydraul 1049 9 0.143 - - - - 0.0513 - 12.0 Pydraul Aroclor 1047 1E47 - 1256 Aroclor 1247 - 1252 Aroclor 124-9 - 1252 10 1 2 3 0.100 0.113 0.144 0.125 - 0.124 lb.3 - 0.C0203 0.52 - 0.121 33.9 0.111 14.6 0.0x36 0.147 101.9 0.0773 0.0012 10.2 0.31 - -- -; - - 0.24 ' 216.7 ; - -. 0.03665 33.0 1 - - 0.0016 1.4 .] 0615663 :* _______ __ . .............................. ..... ........ TOXSTUDIESOI42 facie ii vfa^.e 3) Co N p Katerlal In Centset With Inconel Variation in Teraperature of Inconel F. fijcperiuwnt .Nunber Material Added ral e/riin. impressed as ./l. on Oppor lire and p.p.m. on Xxnror Hat Aroelor Phosgene liCl (uncorrocted for Phosgene) Tricreeyl Phosphate Free Creed* Carbon SSoaexlM Arcelor 1252 4 0.130 C.102 - 13.4 - - - 0.00071 0.7 Aroelor 1249 5 0.069 0.0573 0.0109 0.0143 - 7.5 2.8 9.9 - 0.0252; 22.7 -4 Aroelor 1252 6 0.067 O.O307 o.ooiS - - - 0.0386 9.1 0.4 34*8 I Aroelor 104-9 - 1053 8 0.121 2.17 0.00035 0.0224 - 285.8. 0.09 15.5 - 0.0260 23-5 j Aroelor 1049 - 1053 7 0.122 1.80 0.00071 237.1 0.18 oo H - - o.o3oe 27.8 ; Aroelor 1C49 - 1051 9 0.121 1.37 0.0C037 0.0253 - - 0.0146 180.4 0.09- 17.5 i3Jf ; Aroelor 1049 10 0.120 1.24 - 163.3 0.0190 13.2 -- - 0.0220, 19.8 1 ' --4 0615664 TOXSTUDIES0143 Table h 4) Co t'a * Li Notarial la Contact With Inconel Variation in Tenpers. lux*g oi Inconel O f. axporinent S'jnber fkitorial A:.ded pil./rnin. Kxprestfod &e ..k m/\* or. Jpp-er Line and. p.p.n. on Lover Llfii Aroclor Phosgene 2C1 {uncor- roctod lor iiiosj^ars) iricreryl Phosphate Cresols Carte* Monoxiai Aroclor 10S1 11 0.120 0.S1G - 120.9 - - 0.0211 19.0 Aroclcr 104.9 " 1051 12 0.058 1.60 0.00052 0.0171 - - o.oaS 210.7 0.13 11.9 23.2 j Aroclor 1050 - 1052 13 0.068 1.31 172.5 - 0.0X07 - - 0.0X81 7.4 11.6 i Trleresyl 1240 - 1258 fhoaphata 15 0.11*4 - - - - 0.0049 1.1 " 'i Trloreeyl 1249 - 1252 2 0.122 - - Kjoeplmt* Trlcreayl 1247 - 1253 1 0.121 - Pboephate Trlcreayl 1257 fhoaphate 4 0.233 - - Tricrecyl 1249 Phosphate 6 0.210 - * - 0.0013 0.00266 0.029J 0.9 0.6 26.4 i - 0.0088 c.00158 0.03W 6.0 0.4 31.1 S - - 0.00676 1.6 -( - -. . 4 0615b65 j ________ ,-.U^ TOXSTUDIESOI44 Xable X [ (' e 5) FlO - N r/4 4 i dxprei'c ad as : */l. on Upper Line and p.>.ri. on Lower UB*j Haterlsl In Contact With Inconel in i'e^ersture of Inconel o y. hxperli^ent Siraber Material Added rzl ./mxn. Aroelor rhospen i'iCl (uncor rected for fhostne) fricroayl Phosphate Free Cresole ?! Cerfcon ' 3enoxiA* Trierosyl 1245 Phosphate 8 0.071 - - - - 0.0011 0.3 'I Triersyl 1258 Phosphate Trlcresyl 1045 - 1057 Phosphate 7 14 0.072 0.130 - - - - - .... ^ - - 0.011 2.6 1 Trlcresyl 1049 - ioSi 13 0.125 - - - - 0.098 mI phosphate 23 Trierosyl 1025 phosphate 3 0*120 - - - 0.550 o*o565 0.0250 37.8 13 22.6 trieryl 1053 Phosphate 10 0.175 - - - - - 1 Trlcresyl io5i Phosphate 11 0.195 - - - -- Trierosyl 1049 - 1051 Phosphate rrioresyl 1051 Phosphate 9 12 0.088 0*0^2 - - - - - - 0.35 81 - ~ -' TOXSTUDIESOI45 Cc Tnc '*oi fcElity, UML tl*. of ' '.arrival. a"u Cimroc in lei; lit aS Auir*0.s --Xposad fco Aerosols * or- o.T by jr&o.. I v- i vdranl r -9 into an in sonol lube . Ueatoo to..Z-liZkSZi.ture of 1250 0. (677 0. ) Sg>er- Duration of laent 5umber j^xxpiOoUsTuEre Identification Xunber Initial Weight J&. Change in Weight impressed as Percentage of initial eirbt During Pariod of hxposuro Aftor Period of Exposure Pat as Doted at Led of beat Period of i-posur Length of Survival of Aniraals That . Died (D) or Were Killed U) After 6e*t Period of Exposure X 6.0 + 6.25 Cat A37V . , 2.9n5 -1D.0 6.0 Guinea Pig A759 C.020 1.2 6.0 + 6.25 Guinea Pig A7&Q 1.020 -11.1 6.25 3 200 6.0 + 6.25 2 Mic ) ) Av.C.C24 6.0 + 6.25 Babbit P93<? 2.620 -- 6*0 6.0 + 6.25 Rabbit m3 2.530 - 6-.2 6.0 Hat is301 0.207 - 1.0 6.0 Hat E302 G.20S - i*h +11,2 + 1.6 +I0.C Survived iid survived Plod x'-rvived v urvived Died -'led 7 days X 7 days K 7 days X 7 days X 2 6.75 Cat A379 3.170 - 7.9 + 3*3 Survived 2 months X 6.75 Su.nea Pig A?61 o.45o -1.8 - Died - 6.75 Guinea Pig A762 0.465 0 - Died - 6.75 i-touse A4.35 0.025 - C.o +16.0 i-vrvived l6 days 6.75 3 Mice Av.0.020 - - Died - 6.75 Rabbit F934 2.267 0 +11.2 ; ..rvived 0 days X 6.75 Rabbit P935 2.270 0 + 1.4 Survived C days S 6.75 Rat K303 0.160 - 2.2 - led - 6.75 fiat 6304 0.205 - 4.9 eied 0615667 ^- ; j TOXSTUDIESOI46 iablo 5 t 2) Exper- Duration of liont Exposure Identification Ruraber Hours Xuabor Initial Height kg. Chart: in fcei'Git iixpressed as Percentage of Initial weight During period of Exposure After Period of Exposure Fate aa Noted at Last Period of Exposure 1 Length of Survival s of Anlmala 21sat 1 Died U>> ox? Vara Killed <X) After Last . Period of Sxpoeis'*' 3 3 3.X Cat A370 3.0c0 - 7.5 +10.0 Survlrad 7 *** ' K 3.X Guinea Fl A763 0-4S5 - 2.7 Died 3.1 Guinea Fix; A764. 0.!(.80 - 7.1 + 9.2 .'lurvivod 7 day. Xj . 3.X 3 "lee Av.0.015 -. - Died ri 3.1 2 -lice At.0.0175 - - survived 15 day* X ' i 3.1 Rabbit F93& 2.170 C + 6*0. Survived 7 day* s ; 3.1 Rabbit P937 2.470 - 6.6 . Survived 2 day. 3-1 Rat 2305 C.200 - 3.3 _ Died 3.1 Eat 306 0.200 - 1.0 - Died k 0*9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 * 0.9 Oat A3vl Guinea Pip A765 Guinea Fi : A?66 4 Mice 1 :<ous Rabbit F93o rabbit F939 Rat 1307 Eat 308 2.325 0.640 O.Ji.10 Av.0.017 o.ci5 1.998 2.030 0.205 o.ise - 1.9 1.6 0 - 0 0 0 - 6.8 -10.6 + i.C _ 4 ^*9 . +53. S +27.1 +27.0 +25.6 +32.4 -urvived Died survived Died Survived urvived survived .. urvived ; urvived 9 days -- 15 day* - Ik days 15 day* 15 daya 14 daya 15 daya X X X X k x x ; ; : ; 5 0.25 Guinea fir: A767 C.4S0 - 2.2 + (-.6 survived 2k days x 0.25 Guinea ki A763 0.460 - 4.8 +10.7 : survived 2^. days * X 0.25 5 Mice Av.0*0156 0 + 8.3 survived 26 days X 1 0.25 Rabbit fifli-O 2.115 *37.9 Survived 26 days X 0.25 Rabbit F961 2.050 0 26.5 Survived 28 daya X i 0.25 Rat 309 0.167 -14.4 +36.9 Survived 28 daya X > ; 0.25 Hat 310 0.188 - 6.5 +30.3 survived 26 days X * i 0615668 TOXSTUDIESOI47 fable 6 ' PC !32NTIA L The .`ortallt/. Lon,. oh cf j-urvival* md Charyeg in -ei; ht of AniasEls cjoosed to Aerosols I'c-rraed by .>ro^>liv I-drauI F-y In to en Inconel Tube Keatcu to s i.er-rperature of 1050Q F. (566 C>) Sapor- Puration of ijaent Exposure Identification Suaber Lioure liurcber Initial Weight k. Cli&n-e in V.ei^ht impressed as Percentage cf Initial .el^Jifc Puring i Iiod, cf ^uxposyre After Porit-d of ure Fate as >roted at Last period of ii^posure Length of Survival of Animals 2hat Pied (P) or Were Killed (&) . After Leet . Period of Expoaure 6 6*5 4 6*8 Cat A376 3.0S5 - 7.3 6*5 6*5 Guinea fig a?69 Guinea Pis A770 0*14.50 0.400 - 5.3 - 7.1 6*5 4 6*8 X Kouae } 6*5 4 6*8 1 Kouae } Av.0*03.84 . - 6*5 4 6*8 3 Mice } _ 6*5 4 6*8 Rabbit F942 2.190 a. 6*5 4 6*8 Rabbit F943 2.140 -14.4 6*5 4 6*8 6*5 4 6*8 Hat 311 Bat K312 0.235 0.208 -20 .t - 4.3 7 6.8 Guinea Pig A771 0.490 - 5.9 6*8 Guinea Pig A772 0.465 - 1.3 6.8 3 Mice ) 6.8 1 House ) AT.0.017 _ 6*8 1 Mouae ) -- 6.8 Babbit F944 2.055 - 5.1 6*8 Rabbit P'945 2.140 0 6.8 Rabbit P94& 2.410 - 6.8 Bat 326 0.230 -13.0 6.8 Rat 327 0.280 - 8.6 -22.6 _ +33.2 . _ + c.4 +23.5 -12.1 24.6 -28.6 Survived survived Died Pied Survived Survived Survived Survived Survived Survived Died Died Sled Survived Survived Survived Survived Survived Survived Survived 3 aontha K 16 bours D 3 days 7 day* 17 day* 4 days 3 days 24 daya _ P X P D P K - . 3 daya D 5 daya .D 24 daya 28 daya 6 daya X X .29 daya X 1 nantb . l 0615669 TOXSTUDIESOI48 Sable 6 Ua. 2) CC:;-;D-NTtAE. \ Sscpar- Duration or Insnt Exposin' Identifio&tIon Btaafoer Hour 5wjber Initial Weight * Chan. in V.eirht kxr,reseed as percezitare of Initial seihfc During Period cf kxpoBure After period of ^jq-osure rate an looted at iSd of Last Period or -exposure Length or Survival ; cf Animals That ' Died (D) or Veer* ` Killed (K) After Last t Period of ftepotrer* e 34* Guinea Ri^ A773 Galj-CO - 3.1 _ Died _ . 34*. Guinea Fi<_ A77U 0.435 - 3.4 - Died --, 3.4 3 Mice ) - - Died - ' 34*. 1 rtouse ) Av.C.023 - - Survived 6 days O . 3.4 1 Mouse } - Survived 6 days E * 3.4 Rabbit F953 2*000 -26.2 +50.0 Survived 1.6 zaontfa* 34 Rabbit F9i4 2.250 -23.1 +49.6- Survived 16 days 3.4 Rabbit F955 1.940 -11.6 +38.1 Survived 28 daye X 3.4 Eat 2328 0.230 - 7-4 +24.7 Survived 27 daya X 3.4 Hat 329 0.230 -12*6 +35.7 Survived 27 daye 9 0.9 Guinea Pig A775 0.430 -- 1*6 -36.2 Survived 19 days 9 0.9 Guinea Pig A776 0.405 - 3.2 - survived 1 day X> 0.9 2 Klee 0.9 3 Klee ) ) . Av.0*0162 _ Died survived 4 days K 0.9 Babbit 1956 1.934 0 +27.5 Survived 26 days . 0.9 Babbit 2957 2.180 - 0.7 +28.5 G arrived 26 days E 0.9 Rabbit F9So 1*870 -17.3 +36*6 Survived 1*6 nenths K 0.9 Eat 330 0.260 0 +30.8 Survived 27 days 0.9 Rat 331 0.230 0 +34.8 Survived 27 days 10 0.25 Guinea pig a777 0.645 - 7.2 +11.7 Survived 1*7 nontiss Z 0.25 Guinea Pig A778 0.695 - 6.2 + 6.0 Survived 1*7 nozxths K 0.25 5 Mice AV*0*0122 0 . Survived 3k days 0.25 Babbit 959 2.040 - 0.7 +35*5 Survived 28 days X 0.25 Rabbit 960 2.095 0 +43.5 Survived 28 days X 0.25 Rabbit F961 1.735 0 +29.9 3ui*ViVod 20 day* K ! 0.25 Rat 332 0.200 - 7.5 +57-5 Survived 29 days X 0.25 Bat 333 0.235 -10.6 +42.1 Survived 29 day* X 0615670 i . i . 8iiJ TOXSTUDIESOI49 Table 7 The Mortality, T^cn t'.x of .vurvival, end Chang es in W'ol.bt of Anlgsals Kxpcgod to Aeroncls ror^d by Jropyln^ Aroclor 124-S Into an Inconel Tube Heated to a Genera tare of I20 r~. (677 C.) Exper iment Ifumber 1 Duration or iixpo&'ure Hours 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 Identification Kuzsber Guinea fir A779 Guinea A7C0 5 Mice Rabbit F962 Rabbit ><,63 Rabbit F564 Rat 6334 Bat , 335 Initial Weight 0-415 v^.75 Av.0*01^4 2.105 2.205 1.960 0.220 0.250 Ciianr e in Weirht impressed as i-ercenia^e of Initial Wei/Jit During Period of Exposure After Period of Dxpocure - 1.2 - JL.1 -1.7 -1.4 0 - 6.6 - 6.C _ - _ - * Fate bb Hoted at iind of Last Period of .exposure bled (1) .'led (1) 5 Bled bled (2) Bled (2) tiled 12) Died Died length of Sarrival of Aniaala *2hat bied (D) op Ven Killed (K) Altar Ult , : Period of Exposer, i 1 . .. - ;j ** A 2 3.0 Guinea fir A7*32 0.430 -29.6 Dux-rived 9 day. B : 3.0 Guinea Pig A703 0.440 " 4*5 - bled (3) - j 3.0 5 Mice Av.0.0152 - 6.6 - 5 mod i 3.0 babbit F9oC 1.750 -19.1 + 7.4 -urvired 27 day. 0 3.0 Rabbit F901 1.600 - 9.2 Survived 6 day* 17 3.0 Rabbit F952 1.865 _ - Died - `` 3.0 Rat i-336 0.265 -1.9 - Died - 3.0 Rat a337 0.240 - 4*2 " Died - 0615671 TOXSTUDIESOI50 Vabie 7 u fi: e 2) c i liXp!*- Duration of iwent axpcsure Identification Kutaber Hour's latcber Initial weight 3 1.0 ..uirwsa xL;. A7rH 1*0 Guinea xl - A?i-S .3.0 1.0 1 l-JOUCO ) 1.0 1 Gov; so } av.c,016 1.0 1 House } 1.0 1 louse ) 1.0 Hobbit ?vC3 1.0 Ilafcbit F9vlt i.7 5 1.765 1.0 11abbIt V V 05 1 l`OV 1.0 Sat .-,33S o.4'.` 1.0 *?at u339 o ,26> Cluui:,e 1 n .-rei^ht Hij/fefseu a i-rcoi<tauo of Ini01 al . ei;.ilt During 1 eriuci oi` ,^:..cs.uro lor i-oricc 01 .ci-ure .ate cs Hoted at .+ni cf Last 1ericd or wxpoture - 4.1 - - - - 0 c c - V.it -10.6 . -- *>o.2 - - - - +2-3.7 +2 3 +: 5.0 +30.3 +30.9 .-urvived urvived - urvlved . urvlved .urvlved 1urvlved urvlved -urvlved Hurvivod urvivod urvlved ;al Lornth. of Survival of Anir.ala that. Died ^D) or fcere. allied tX) After Last Period of Lxposura 7 hours D - ' i days D . 5 isinutea D 1 day D . 6 days D 6 days 0 1 23 days X ' 23 Says K ; 23 days K 24 days K > Zk days X 1 0.33 Guinea ?ir A7C6 0.i;60 - 0.7 riurvivod 1 day D : 0.33 Guinea ri/; A7G7 ij.ij.6C - ->0 ,0 urvlved. V days D : 0.33 5 Mice Av.0.01c2 -22.0 3 urvlved 1 day O * 0.33 K&bbit r`9Gp 2.075 - 1.1 + V.- O nurvivod 6 days X 0.33 Rabbit Fy66 1-3U _ -13.2 -urvlved 16 days D 0.33 Rabbit F967 1.9&0 - 1.0 - survived 6 days S 0.33 Rat 3*4-0 0.220 0 + 6.5 urvived 13 cays' X 0.33 Rat B3U. 0.225 0 * 3.6 ...urvlved 13 days X 0615672 TOXSTUDIESOI51 Sable 7 (Fc^e 3) v.C 1 D NT! AL Exper Duration of Iment ^xpoure Identification Furaber Hours Humber Initial k. Chan; e in Weight expressed as ^ercenfca^e of Initial aei&ht During i-eriod of i.x.posure After k'oriob of exposure Fete as Hoted at Gnd of last Period of Exposure ooooooooo 5 Guinea Pis.; A?C 5 Guinea fir: A?C9 0.140 0.l5 -52.0 -53 >3 -.Vorvirod Survived 5 ice 1 iou.se ) ) av.O.CIGG -25.5 -23-7 Died -urvived Rabbit F9ov Rabbit PV69 Rabbit 1970 2.160 1.595 1.C70 :ia -13-6 :i:I Survived -urvived Survived Rat K3u2 0.260 - k.e + G.G Survived Rat 131*3 0.215 - 4.7 Hied benct'a of Surrival of Aniaala Tbat Died (V) or W.A Killed (K) . After last ; Period of oxjjoeur* 9 days 0 10 daya 0 4 days B 22 days K 22 daya 7 day* 12 days K , , 6 1.0 Guinea FIp A?90 0.605 - .5 1.0 ouiaoa tS.' A79X c .425 - 9-5 -11.2 + 7.5 Survived Survived 1.6 months X 1 south K 1.0 5 Mica av.C.0142 1.0 Rabbit 5971 1.795 1.0 ftabbit 1972 0. 0 c *55.9 +24.0 -3 - 5 Survived survived .-urvived 2b daya K 22 daya S 1.4 months t 1.0 Rabbit 1973 1.0 Rat 1344 1.600 0.330 0 0 +17.3 - 3.6 -arrived .urvived 22 daya X 23 days K 1.0 Rat h.345 0.295 0 +21.6 . -urvived 23 days K <1> 2.25 hours im the start of w^ot ur. (2) 6 boors fr;.;s the start cf e^porure. (3) 1*5 hours fror. the start oi e^o?ure. 0615673 l TOXSTUDIESOI52 i'ahlo C CON? Hhe Mortalitya -Lors z':i :X -urvival, and Chen os f.n height of Animals exposed to Aerosols i-oraed by Drop,,in ; Aroclor 12kS Into r.n Inconel fu.be liested to c. -^noerature cl' 1C90G r (>66 C.) l iixpem duration of inent >ocporure IdontIX*ice. t Ion Humber '.lours Mujiber Initial weight k^:. Change in /.`eipht j^xpreesod as I-crcorstnt 0* initial Aei';ht Duping period of ;ooo*uie After Period of iate as i-oted at Last Period of exposure Length of Survival cf Animal* That Died (D) or Wera Killed (X) After Last . Period of kxpoaure 8 7.0 Guinea A79k 0.435 - 2.3 7.0 Guinea f it_ AYvb 0.460 - 2.2 7.0 7-C + 7.0 ^ Mice i Mouse ) J Av.0.0236 7.0 +7.0 Ktbbit >977 2.060 - ?.2 7.0 + 7.0 Rabbit FvYC 2.200 -10.0 7.0 Rabbit F979 2.175 - v.2 7.0 Ret ii36X 0.260 - 7.7 7.0 + 7.0 Rafc 0.295 -32. & Died Died k Died urvlved Died Died Died Died Survived 12 hours & $ daye D 7 7.0 Guinea A792 0.450 0 Died 7.0 Guinea Pip A793 0.420 - 1.2 - Died _ 7.0 2 Mice } - _ 2 Died _ 7.0 1 Mouse } AT.0.019 -24*3 7.0 2 Mice } - - Survived 3 day* D - Survived 26 days K 7.0 Rabbit F97J; 1.966 - C.5 - Survived 5 <Uy. P 7.0 Rabbit F973 1.665 - 9.4 +14.2 survived 1.3 sraUi. X 7.0 Rabbit F976 7.0 H*t f-359 2*003 0.305 - c.l - 2.6 +194- survived Died 1.3 Math* _ X' 7.0 Ht i360 0.310 - 3-2 " Died 06156?* TOXSTUDIESOI53 Ti A L Fjcpor- .Duration cf toant Kxpo#ure Identification Sunbar Hour* Ihirsber Initial ftei^bt ks- Chang e in weight ^pressed as rorceuta^e of Initial Weight During Period of kxpQBvre AX'ter Period of exposure Fate aa Noted at 2nd of Last Period of ibepocure L^in. tl\ of Suty1t1 cf Anial* Xhat Died (D> or Kqr. Killed <K| After Last .. reriod of BxfOtatrm 9 3-5 Guinea. Pis AT/6 0.350 _ Died ^' 3-5 ulcos ric A797 0.415 3.5 3 aloe ) - - Died - 3 Died 3.5 1 .louse ) AV.0.019 - Survived 10 days $ 3.5 1 House ) - $arrived 20 day* 3.5 Rabbit F9&& 2.320 -13.6 +15.6 Survived 1 znontfa 3.5 Rabbit PVo? 2.?50 -- - 4.3 'survived 5 day* P 3.5 irabbit i'vUC 2-355 - 0.6 - Died -- 3.5 Kt K3&3 0.290 - 3*4 +23.1 Survived 1.1 nopthe X 3.5 Hat E364 0.240 -10 .c +26.2 Survived 1.1 raonthe .10 1.0 Guinea Fig A7r/o 0*45> - 2.2 Died 1.0 Guinea Pi A?99 0.430 - 3.5 Survived 2.6 hour. B 1*0 5 Mice Av.0.0190 - 2.2 +37.9 Survived 20 da?* 1.0 Babbit Fv89 2.040 - >. - 6.9 Survived 6 days P : 1.0 Rabbit 2.2oG - 3.5 +17.3 Survived 1 sooth 1.0 Hat .365 0.315 - 4.8 +24.S Survived 1.1 wonthe l.C Sat 366 0.305 - 4.9 +16.4 Survived 14 day* K .11 0.33 Guinea Fli. AGCO c.iao -45.1 Survived 10 day* B 0.33 Guinea Fi;' ASGl 0.455 -1.5 Bled - 0.33 5 Hice ' Av.O.OoO -25.6 +45.6 Survived 19 dajrrf K 0.33 Sabbit F9V4 2.095 -11.2 +24.3 SJiTTiVOd 1 .neath 0.33 Rabbit F99> 2.120 - 3.2 -21.4 Survived 1.3 X 0.33 Habblt F996 2.1VO -26.1 Survived 12 days D 0.33 Sat i-367 0.280 0 <29-6 arrived 1.1 matt* x 0.33 Hat 366 0.255 0 +3S.7 r-urlired 1.1 mbU. X 0615675 TOXSTUDIESOI54 Tabic 2 {r&l.Q 3) S-Q.nfjoeNtial xperIsont Ifrasber 12 duration, of Exposure Sours 3.5 3-5 3.5 3*5 3.5 3.5 3.5 3.5 Identification Kuaber Guinea Pig aGQ2 Guinea Pig A603 5 idico fiabblt B997 Rabbit F99o Rabbit F999 flat 369 Bat E370 initial Woi-dit kg* 0 .1^00 0.450 Av.0.017 2.210 2.080 2.090 0.255 0.235 Change in Weight Expressed as Percentage of Initial Weight .During Period of Exposure After Period of Exposure - 3.3 - 2.2 0 - 7.2 - 5.5 0 0 _ - +22.4 +20.5 +11.3 - 5.7 10.9 +26.7 Fate as 40ted at jsUd Of Lest Period of Exposure Died Died Survived Survived Survived Survived Survived Survived Length or Survival of Animals That Died (O) or Ner*" Killed (K) After last period of JKxpo*ure ^ -- 16 days X" 29 days X 29 days K 5 days 0 1 mouth K 1 raonth K _ . 13 . 1*0 Cuinea Pig aG04 0.425 - 2.4 Survived 1.0 Guinea Pig a0 0.^05 - 1.0 1.0 3 Mice 1.0 1 Mouse ) ) Av.0r .O- -X1 -/r 0 ' _ Survived - Survived _ Survived 1.0 Rabbit 02 2.340 -15.6 ' +40.6 Survived 1.0 Rabbit G3 2410 -- 6.6 +24.8 Survived 1.0 Hobbit 04 2.345 - 3.2 +124 Survived 1.0 Rat E371 C.230 - 6.5 9.0 Survived 1.0 Rat 372 0.215 - 2.3 7.4 Survived 3 hours 0 3 hours 0 16 days K 7 day# D 37 daya 29 day* X 29 days X 32 daya X 32 day* X 0615676 TOXSTUDIES0155 Table 9 'f ? i D [ * ~ IA I The `iortalit-, Lcn." ch cl Survival. and Chargoe In lit c-f /uili:&2s ajcyosod to Aerosols Fomccl by ^roupliv glcrcryl i;ho&u:,g.Le Ir-oo an Inconel Tube Heated to a ici-feyorgitv.ro of 1230 i * 1677 0) Exper- Duration of loont Sjp-osure Identification Sutler Hours Suraler Initial itelghfc kg- Change in Weight uxprested as Percental e of Initial +'cigat Turing Period of h-xposure After period of ure Fate as Foied at v i- eriud of i-sposurs Lesrtls of Survival oi Anircals That 6ied (D) or wap. Killed (X) After Last i'erlod of ixpocux* IS 2 x 7.0 Cat A371 3.611 - 6.5 2 x 7.0 Quines fir aS62 - 4.0 7.0 Guinea Tig A063 0i|3b - 2.3 2 x 7.0 5 sloe AY. 0.0154- - 2 x 7.0 Rabbit G178 2.963 - 6.4 2 x 7.0 Babbit 0179 2.543 0 2 x 7.0 Babbit GlSO 2.791 - 4.9 7.0 Rat E479 0.406 - 2.2 7.0 Rat &M 0.450 * + 1.2 +11.9 - +12.9 -lg.6 +19.6 -- " .survived survived Died 3 Died -Survived Survived Survived Pied Pled 7 daya K 22 day* S. - 17 day* S 14 <!aya D 16 days JL - * 2 7.0 Guinea Tig A0O6 0 ..T10 - 4-9 -35*0 survived 30 days D 7.0 Guinea Tig A&Q9 0.495 - 1.4 7.0 S .'lice AV.0.017 - .0 - Tied - 3 Pied --y - 7.0 Rabbit uS 1-945 - 6.2 +3G.6 Survived 27 days K 7.0 Rabbit G9 2.010 - 2.7 +22.1 Survived 27 daya K 7.0 Rabbit G1C 2.C75 0 +33-5 Furvivod 27 days IS 7.0 Sat E37S 0.195 -14-9 - Pied - 7.0 Rat E376 0.225 -12 .0 Pied 061567? T-- TOXSTUDIESOI56 ^2i2CC:;?i0ENT(A Sxpar- Duration of inent Ksposure Identification Hissber Hours Nutter initial Weight k# 1 3.5 Guinea Sic A8G6 O.pOO 3.5 Guinea li^r A607 C J*20 3.5 2 Mice ) 3-5 1 Mouse ) Av.0.017 3.5 2 /ice ) 3.5 K&bbit 2.270 3.5 Rabbit 06 .110 3.5 Rabbit G? .140 3.5 Ket 0373 0.220 3.5 Hat 374 0.215 Chance in Weight Expressed as tercenta&o of Initial Weight During reriod of .ucpoEur After fcrioi of Exposure - 2.6 - a. -10.0 c 0 - 0.5 0 0 - - - ++133*.35 +37.4 +20.7 + 2.3 + .3 late as Goted at End cf Last laried of Exposure Died Died 2 Died o-arrived Survived survived Survived Survived Survived Survived Lenetix of Survival of Aninals That Died (U) or War-- Killed U0 After Laat ferlod of Expoaur. _4 -- -- 22 day. D 34 day* K 28 day S 28 day. K 28 day* K 34 day. K >4 days JC . 4 1-0 Guinea Fir AS22 0.145 - 1.6 1-0 Guinea rip a23 0.430 - 5.3 - Died Died - 1.0 5 nice Av.0.0150 - +57-3 survived 22 days X 1.0 Rabbit G22 .095 - 2.0 +32-2 Survived 19 days X 1.0 Rabbit 23 1.995 - 2.3 -19.5 Survived 12 day. 9 1.0 Rabbit 24 2.110 - 5.9 +20.$ Survived 2$ days K 1.0 Rat 399 0.215 - 6.0 + 0.4 survived 1.1 months X 1.0 Bat B400 0.210 - 3*6 +16.7 Survived 1.1 months & 6 0.33 0.33 Guinea i'i:- A842 Guinea fir A-s43 0.905 c.Oco - 1.1 0 1. #!| + 3.9 Survived survived 6 days X days It 0.33 0.33 5 Mice Rabbit 0134 Av.0.022 2.450 0 0 +L. .5 15.7 survived survived 11 days.lt c days X 0.33 0.33 Rabbit G117 Eabblt OllS 2-2>0 .260 - 9.1 -- 5*2 +3.9 + 7-3 Survived - urvived 1-1 nonths X 6 days X . 0.33 0.33 Hat 437 Hat 438 0.175 0.205 - 5.7 0 +20.4 survived survived C days X $ days X ' - - ---------- 1_ 0615678 TOXSTUDIESOI57 i'calc 9 3) CONPiD2.-;rr a r SXpoT*- Iteration of knent iscposur Identifice tion Kunber &e>urs dumber Initial Change in ;.i-4hb ricprecoocl a e Percent?.' e or Initi al ioir.ht burins ruriod cf _urposure After Period of exposure fate as ;.otd at knd of kast furled. of ^poeure Len,- t i of survival of AnlssaIs That Di&d (V) or 'Were Killed (5) Aftor Last Period of kxposur* s 3*5 Guinea f AC46 1.060 0 3-5 Guinea Pi^. Aok7 0.770 -7.e 3.5 2 Kies ) - 3.5 2 Mice ) Av.0.02if - + 6*3 *13.2 - -3.0 Survived survived Died iurvived 7 day* X 7 days K 7 days D 3.S 1 House } - - Survived 7 days & 3.5 Rabbit G129 2.3i> -0.2 - 7.0 Survived 7 days K 3.5 Rabbit 0130 1.V20 0 + 0.3 : urvivod 7 days K 3.5 Rabbit G131 l.v55 0 5.2 Survived 7 days K 3.5 Rut 4^1 0.315 - - oied - 3.5 Hat E!(42 0.335 -7.5 9*6 survived 7 days K 7 1.0 Guinea PI*; A044 0.355 0 1.0 Guinea i-i^ A64S 0.G70 0 + 2.1 3.1 Survived Survived 2 days C days 2 1.0 5 ,Hce Av.0.023 -6.0 1.0 Rabbit 0119 -6.8 lu.> +19.1 Survived survived 5 days X 2> days X 1.0 Rabbit 0120 2.325 0 10 .k Survived 8 days K 1.0 ' Rabbit C121 1.0 Rat K!t39 2.Wo 0.195 -7.3 -5.1 35.0 +4.6 -V- arrived Survived 1.1 months K S days 1.0 Rat &1-40 0.190 0 +23.7 survived G days K 0615679 I ------- .1 TOXSTUDIESOI58 Cable 10 C O ^ iN t I A L The r-iortality, Lcn. th cl* 'vsnriv&l, and Ohanreg in height or Anlaals i^poged to Aerosols lorded by Dro,-,r.-iry. Triorogyl ihogpriato Into an Inconel Tube Heated to a revere jure of P. ($66 C) Exper Duration or iment i^xpceur Identification Jhrabor Hours J*urxcer Initial Weight Change in UoLht Rxprefised as fercontoge of Initial height During Period of Exposure After 1 cried of ibeposure Fate as Hoted at Had of Last Period of Exposure Length of Survival Cf Anlwala That Died (D) or Verm Killed () After latet Period of Exposure 14 7.0 Guinea Pig Ac60 1.03S - 4.0 7.0 Guinea fie AS61 0.t63 - 6.0 7.0 5 Mice Av.0.0208 -23.1 2 x 7.0 EaObit G163 2.393 - i` .s 2 x ?*0 Rabbit 0164 2.029 -12.3 2 x 7.0 Rabbit G165 2.005 -10.7 7.0 Eat K471 c.454 -10.1 7.0 Rat =472 0.474 - 5.9 13 7.0 Guinea fir AC50 0.430 - 4.2 7.0 Guinea Hi- Ao59 0.410 - 1.2 7.0 3 ;:ico 7.0 2 nice ) ) AV.C.Q16 _ 7.0 Rabbit C-1SX 2.040 - 7.7 7.0 Rabbit Gl>2 2.525 -10.5 7.0 Rabbit Glb3 2.035 - 6.4 7.0 Hat Si.63 0.470 - 4.0 7.0 Sat 464 0.455 - 2.2 _ _ " . _ _ 16.9 13.5- - * Died Died Died Died Survived Died Died Died Died Died Died Survived Survived survived Hied Died Died - -- -- 1 hour D - - - _ 13 days X 12 day* K 13 days K ' - 0615680 j TOXSTUDIESOI59 fable 10 {fa/e 2) CC Expert Duration cf iment Exposure Identification Number Houre Missher Initial Weight Change in i.-eicht Expressed as Percentage of Initial Weight During Period of Exposure After Period of jixpooure Pato &a looted at End of last Period of Exposure Lenetb of Survival of Animale That t . bled (D) or >fer* Killed (xj" After last Period of ixpoeurs 3 3.5 Guinea Pie A0l8 0-434 -1.8 3-5 Guinea Pie AS19 0.430 - 3.3 Died Died _j 3.5 1 !;cuse 3.5 3 Klee } ) At.0.0138 -19.3 +44.9 Died Survived 26 days X 3.5 Rabbit G17 2.150 -1.6 -19.0 Survived 7 days B . 3.5 Rabbit 016 1.eai. - 2.5 +23.0 Survived 20 days X ) 3.5 Rabbit 019 2.100 - 2.8 +12.2 Survived 20 day* X 3.5 Rat E393 0.193 - 4.1 +20.7 Survived 25 day* X j 3.5 Rat *394 0.197 0 +12.7 Survived 25 days X < 10 1.0 Guinea Pie A852 1.110 -11.1 3.0 Survived 8 days X 3 1.0 Guinea pi. *853 1.280 - 3.4 Survived 2 days 9 : 1.0 3 Mice 1.0 2 Mice ) ) At.0.018 -22.2 -8 +19.5 Died Survived 7 dlys I 1 1.0 Rabbit Cl42 2.166 -47.8 Survived 26 days S j 1.0 Rabbit C143 2.835 -43-0 Survived 17 days B 1 1.0 Rabbit G144 2.470 - 2.0 + 6.7 Survived 8 days X * 1.0 Eat 454 0.160 - 5.0 19.4 Survived 9 days X j 1.0 Rat B4S5 0.160 - 6.9 Died 11 _ . ,,, 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 Guinea pig a52j- Guinea Pig A855 5 Mice Rabbit C145 Rabbit Gll.6 Rabbit C147 Rat E456 Rat HS7 0*44.6 0.465 At*0*0190 2.400 2.333 2.395 0.450 0.450 - 5.5 - 1.7 -13.7 - 2*2 - 6*4. 0 - 6.0 - 9.1 +15.9 + p*6 +17.9 + 3.5 +11.6 +10.9 + 2.4 17.8 Survived Survived Survived Survived SurriTed. Survived Survived Survived 9 days X 9 days X 10 days X 8 days 8 day* X 8 days X 1 9 days x . 9 days X i 1 1 j 1 | j | TOXSTUDIESOI60 Pablo 20 (PeLe 3) C O ,7.7 ;;: ' L Kjcper- Duration of tBAnt Exposure Identification Buaber Hour luasber Initial Weight Change in veight Expressed as Percentage of Initial Weight During Period of Exposure After period of Exposure Fate as 'doted at but Period of Exposure length of Survival of Aniul* That ' Died (D) op Snt ! mm (k> vi After But ' Period of EXpoeure 9 3.5 Guinea Pig AcSO 0.920 - 3.0 + 0.9 Survived 8 daya Z 3.5 Guinea Pit A651 1.035 - 0.2 _ Died 3.5 3 Klee ) -11.9 _ Died ! 3.5 2 .Mice } -21.4 3.5 Babbit 0139 2.145 - 9.0 +1.4 + 6.3 Survived Survived 7 days Z 9 day* Z * 3.5 Rabbit GILO .430 - 4*9 0 Survived 14 days S 3.5 Rabbit Glia 2.460 0 + 0.8 Survived 4 daja Z 3.5 Rat )4i; 0.360 - 6.7 Died 3.5 Eat ii45 0.330 -10.6 8.8 Survived 9 days Z 1 -I 12 1.0 Guinea Pig a8$6 0.750 - 5.2 1.2 Survived 6 day* D 1*0 Guinea Pig A8i>7 0.510 - 5.3 -17.6 Supplied 29 days B 1.0 5 Mice ATa0.017 0, +11.8 Survived ? day* Z 1.0 Rabbit 0148 2.688 - 8.1 *10.2 Survived 8 dan Z 1.0 Rabbit 0149 1.0 Rabbit 01^0 2.400 2.170 - 2.0 - 1.8 16.4 +15.2 Survived Survived 8 day* Z 23 day* Z 1 1.0 Rat E458 0.460 - 4.8 + 3.0 Survived 9 day* Z 1.0 Bat 459 0.430 - 6.5 4.9 Survived 9 days Z 0615682 TOXSTUDIES0161 Table XI C; TlA l Su^rra-rg- of Mortality Araonr* Groups o! Aaliaals Subjected to Aerosol* Forged by Propplag Pydraul P-9. Aroclor or 'grlcresyl Pnogphate Into a Heated Inconel Tube Compound and Taiaperature of Contact Duration Hour* Mice Bats Guinea PiB Rabbit* Cat* Exjcrlaant Aabar Pydraul Decoapositlon 12$0 F. 6.0 6.2$ 2/2 1/2 0/2 A 6.75 2/2 2/2 0/2 o/i 3.1 0.9 0.25 i 2/2 1/2 1/2 oA 0/2 1/2 0/2 oA 0/2 0/2 0/2 1 2 13 131 8Pydr&ul Decos^poeifcion 1050 P* 6.5 6.6 6.8 3Jt 0.9 0.25 1/2 2/2 2/2 o/l 1/2 2/2 0/2 2/2 0/2 2/2 0/2 0/2 6 9 10 Aroclor Decomposition 1250 F. 7.0 3.0 1.0 0.33 3.0* 1.0* 2/2 2/2 0/2 0/2 i 1/2 o/5 0/2 2/2 2/2 2/2 2/2 2/2 0/2 8 - 1 2 i 1 1 I 4 i i i -i \ 0615684 * TOXSTUDIESOI62 i'eolo 11 Us^e 2) ccn . .... ' i iAL 0615683 i TOXSTUDIESOI63 CONFIDENTIAL Tablo 12 Incideneo of 'ortP.lltT In Relation to Material ami Species ilar.ardloss ol' Ui Toaii-eirature of tbo Inoono^. Spool* Mi oo Total Humber of Animals .(.'bat Died/Total Humber ;&poood rydraul f -9 Aroolor 1248 Tricrosyl Phosphate $ 23/49 (67.1) t 37/63 (93.7) * 32/69 (46.4) Guinea H(-a 13/20 (66.0) 24/26 (92.3) 17/28 (60.7) babbits 4/A (16.2) 16/38 (42.1) 9A2 (21*4) y<&,tn 0/20 (40.0) 9/26 (34.6) 11/28 (39.3) 0615685 TOXSTUDIESOI64 CONFIDENTIAL mu Irialdeno* of Mortality In Halation to :1b tarial. Spbola*. and Tonperatura of Inoonol Spool** Hloe Ouln*a 1`U`t Kabbtt* fiat# Total ."iloa Gulnoa 21c* babbit* fiota Total Mio* 0uln*a PI;;* Babbit* Rat* Total Muraber of Animal* That ijled/tlumbep of Animals r-xposod 1250 P. 1050 F. Aroolor 1?48 ?4/29 10/12 7/18 5/12 $ (82.8) (S3.3) (30.9) (4l.7> 46/71 (>4.8) % 13/34 (38.2) 14/14 (100.0) 9/20 (45.0) 4/14 (20.6) 40/62 (40.8) lydraul 1-9 15/24 5/10 1/10 6/10 % (62.5) (So.o) (10.0) (60.0) 27/54 (50.0) 13/25 S/10 3/14 2/10 % (52.0) (BO.O) 21.4) (20.0) 26/59 (44.1) Triorasyl Bhosptxato 17/35 7/14 2/21 5/1/i % (40.6) (50.0) ( 9.5 (35.7) 31/04 (36.9) 15/34 10/14 7/21 6/14 % (44.1) (71.4 (33.3 (42.9) 30/03 .(45.6) 0615686 TOXSTUDIESOI65 i'&blo 1^. The Toxicity of i'jUravl I~9 !-:h.ei\ cor: tInyo-os1 y for Tventy-fo. r Hours in Contact Wltn the ---'iy. of j'r^-'blts by the Cleave 'isthcd of braize* Woodard &nu salvor? (Applied undiluted) Xdeiiti-- flection irurobor Condities of 3kin Peso ral.Au- Initial Weight Loss of 'eiUt Expressed as rereenta^e of Initial Weight i* ate Length of Survival After the Material was Applied c-333 0-339 Intact Intact 9-4 2.060 9.4 2*390 0-340 G-IU.9 0-421 Intact Intact Intact 6.0 2.275 6.0 1.630 6.0 2.018 G-2i20 0-436 0-439 Intact Intact Intact 3.6 5.100 3.6 2.262 3.6 .6*6 c-455 Abraded 6.0 2.090 0-4S7 Abraded 6.0 1.966 0-458 Abraded 6.0 1.666 36.1 17-4 19.8 9.9 9.6 6.1, 3-8 3-7 36.5 4.3 13.2 died ' Died 7 <5*7* 3 days Died/ 20 days Survived 9 days; killed Survived 9 days; killed Survived 23 days; killed Survived 16 days; killed Survived 16 days; killed Died 01ed died 19 days 44 boars 3 days 0615687 ] 4 \ TOXSTUDIES0166 Table 15 The Toxic It? of Arocior HiiC 'then ^jLlrtalnad Continuously for Tventy-fo.ir iioure in Contact With the Skin of Rabbits by the Sleeve Method of .Praise, Woodard and Calvary . ( Applied imdIluted ) IdOTltiicetion tiuniber Condition of Skin Dose ai-Ar,- Initial Weight kg. Loss of Weight impressed as Percentage of Initial Weight Fate Length of Survival After the Material Was Applied c-348 g<4^o G-441 Intact Intact Intact 9-4 .310 V.J 2.310 9-4 2.033 0-422 Abraded 9.4 1.(565 0-424 Abraded 9.4 1.922 0-443 Abraded 9.0 1.970 0-444 Abraded 6.0 2.196 c-456 Abraded 6.0 1.662 19.3 3-9 26.2 9.2 35.2 14.8 19.2 12.7 Survived 21 day*; killed Survived 21 days; killed Survived 31 day*; killed Survived 4k day*; killed Died $ day* Survived 29 days; killed Died Died 6 days 21 hours 4 3 0615688 j iJm TOXSTUDIESOI67 Table X6 ! N T! A L The Toxicity :.f Trlcresyl r~:iC'Sy~^.tQ vraen r^.intalnod Continuously For Twenty-i'cvr Hours ir? Contact With the Skin of Sahblts by the Sleeve Method of Dralze, v/oodard and Calvary . (Applied undiluted) Identi fication Condition Pose dumber of Skis ml.At'. Initial weight kg. Loss of Weight Expressed as Percentage of Initial Voight Fate Length, of Survival After the Material Was Applied 0-628 0-337 0-334 0-344 0-345 g-423 G--'|42 0-445 Intact Intact Intact Intact Intact Intact Intact Intact 9.4 6.0 5 2.5 2.5 1.6 1.6 1.6 2.487 *(4&0 2,190 2.360 2.125 1.620 1.809 2.158 15.5 16.1 1.0 38.2 9.6 13.2 11.3 5-9 Died 2 days Died 2 d*y Survived 20 days; billed Died 7 day* Survived 6 days; killed Survived 9 days; killed Survived 21 days; killed Survived 16 days; killed 'i 0615609 1 ___-J TOXSTUDIESOI68 4 Sie Toxicity of Car.ioylo WK fther. 0c,nil^3.y.alz "or Twenty-four Hour; In Cosset with. t:io : kin or rabbits by the -'loeve ':sthoa of Ibfalse, noodara and Culver? (Applied -Aadlluted) Identi fication Condition Dose Number of Skin ni-Aa- Initial k^. Loss. of ksd/iit b.-.presi:od as rcrcontccro of Initial Vei^iit fate burn th of S;ur/ival After ulus Material kQS Applied a-329 0-331 6-3*0. 6-3*0 6-343 G-332 0-352 G-3S4 Intact Intact Intact Intact Intact Abraded Abraded Abraded 9.4 9.4 9.4 9.4 9.4 9.4 9.4 9.4 .470 .345 2.030 2.210 2.160 2.140 2.450 2.255 12.9 11.9 1 .2 17.6 10.2 6.6 10.4 20.2 Survived Survived survived Survived Survived I1- days; killed 10 days; killed 20 days; killed 19 days; killed C days; killed survived 9 cays; killed Survived 20 days; killed Survived 20 days; killed 0615690 1.--.- ^---.J TOXSTUDIESOI69 ?0NfideNt;al Guorrarisod Data on tlio Ini-odlste Poxlcit;; of Py-^&u1 F-9 Aroclor Ik, Tricree-yl Phosphate rjr.d Gargoyle Ghen Maintalned vpon the Skin of Rabbits According to the sleeve hofchod of u/ralze, Woodard and Calvery Dosage ni.Ae. i.iE'ibor that i-'ied/JfUEiber Given cho ha ferial PycLraul F-9 .Aroclor 124.8 Tricresyl Phosphate Gargoyle PIi> Intact Abraded Intact Abraded Intact Intact Abraded 9A 6.0 3-6 2.5 1.6 2/2 0/3 1/3 Vi V3 3/3 - 2/2 vi 9/3 - -- - -- -- V3 * " - * , 0/3 0/5 - 0/3 - 0615691 4 > ---------------- 'A. TOXSTUDIESOI70 TOXSTUDIES0171 Figure 2 0615692 TOXSTUDIES0172 Figure 3 TOXSTUDIESOI73 0615695 TOXSTUDIESOI74 1 Figure 5 0615696 TOXSTUDIESOI75 Per cent Trcmsmisson TOXSTUDIESOI76 i Concentration of Tricresyf Phosphote in Ethyl Alcohol,r/ml. Figure 7 0615699 TOXSTUDIES0177 Figure 8 TOXSTUDIESOI78 Per cent Tronsmisson Figure 9 0615700 TOXSTUDIESOI79 Per cent Transmisson Figure 10 0615701 TOXSTUDIES0180 r " FIGURE 11. 0615702 TOXSTUDIES0181