Document N2163Ld7ZoB6Zg3jbwb9DpeEE
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to aFsC-P11F6O,AFaC-m1m2o6n,iFuCm-1s6al9t,, AFCm-m1o4n3,iuormapseraflmuacjroorocctoamnpoaotnee,nPtFoOf, FX-1003. (Octanoic acid, pentadecafiuoro-, ammonium salt,
CAS # 3825-261)
Remarks: The 3M production lot number was 2327. The test sample is FX-1003. It's purity was not sufficiently characterized, though current
ipnefrofrlmuaotriooonctianndoiactaet,es5i0t i%s waatseolru,ti<o3n %ofi<ne4rt5p%erafmlumoorinniautemd compound and 1 22% Cs and Cr perfluore analogue compounds. tTehset fsoulblsotwainncgesiunmwmaatreyr saoplpultiieosntwoitthheitnecsotmspalemtpelleyacshaarmacitxetruirzeoedfthe concentrationsof impurities. Data may not accurately relate toxicity
of the test sample with that of the test substance.
METHOD: Methods: OECD 209
Test type: Static acute
YGeLaPr:CoNmopleted: 1990 ASntaolcyktiacnadl mtoesntitsoorliuntgi:onDpirsespoalrvaetdiooxn:ygeTensctosncuebnsttraantcieonsso.lution was cwarteeart,ed16bymmLassysntahdedtiitciosnetwoaagesofleuetdi,onancodnt2a0i0nimnLg 2i8n4ocmulLumM.ilAiporreefeMrielnlc-eQ sdoilcuhtlioornopwhaesnoclr.eated using a 500 mg/L stock solution of 3,5Inoculum source: Mixed liquor activated sludge collected from Metro WMaisxteedwalitqeurorTrSeuastpmeenntdePidanS,olSitdsP:aul3,.2MgN/L Inoculum holding: None-- used day obtained. Test Co`npdHiotfioAncst:ivated Sludge Mixed Liquor: 6.7 (inital) and 7.1 (final)
PTeHmpoefr1a,t0u0r0em:gl2L0 t2e1stcConcentration: 7.3 (initial).
Element basis: Respiration inhibition as determined by oxygen
consumption.
RESULTS
Nominal concentrations: Bk control, Reference control, 1000 mg/L.
93873
Elementvalues: 33-0h-omuirnuEtCesoEC=s>1=,0>010,0m0g0/mLg/L
All element values based on nominal concentrations.
Rdeesmcarrikbsed: iTnetshteintgeswtassubcsotnadnucceterdemoanrktshefimeildx.tuTrheeofvtahleuetsesrtespuorbtsetdanacpeplays
to that mixture and not the test substance.
CONCLUSIONS The FX-1003 3-hour EC for activated sludge respiration inhibition was determined to be >1,000 mg. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Kiimisch ranking 2. Testing meets all criteria for quality tTehsetirneg,sbautllacakckosfacnhaalryatcitcearliczoantfiiornmoaftitohneotfesttesstamspulbes.tanTchee ctoenscteinngtrations.
procedure was also not fully documented.
REFERENCES
TMhiinsnesstoutday,wLaasbcRoenqduuecsttednubmybtehre
3M Environmental G2882.
Laboratory,
St.
Paul,
OTHER
Last changed: 5/25/00
03874
.
Date = 16-97
. ENVIRONMENTAL LABORATORY
natyee JAR
ACTIVATED SLUDGE MIXED LIQUOR
Type reneQECD ActivatedSludge Recpiration Trhib #209
source Mesto [Uaste Treatment Plans se Paul, my
Date Obtained_Q- Jb- 9D
Date Used_Z-/b-9O
Mixed Liquor Suspended Solids (MLSS) _ 3. \ g/L Adjustment
oi Thisials 6.7 Fined: 7.f
2 8/L
Comments x
=
Zest Substance
Sample Description FX- /003
Sole ploome liz 7:3
Lot #327
majucsent none
-
Comments
Reference substance
:
Sample Description 3, 5- Dich axapheng
Sample PHSicek Solu 3 7,5 Adustaene_A/ one
Coments_ S20 pall
Exposure Conditions
Test Duration
3-Hours
Test Temperature Ao- 2
%.
-
coments lest (un touk, iR 4/234
--------------------------------TO--3--87l5
[--
Lab Request No. 2.22 ZR
Sample Description
Clear Lig id
Fx-1003 Lot 4327
Environmental Lab -- WorkSheet
OECD ACTIVATED SLUDGE
RESPIRATION INHIBITION TEST #209
fest Solutims B
g
"0 La 3 oats ___A-lb-9D
<4R
Analyst:
e ----S ------------"--------------------------------
eee------------------e-- ee --------e--e--r -- er
Seem --erere
ester teres
Test Conc. mg added
Blane
-
loco
S00
errr
Thitiel pH
_mlmp water ml syn. sewage
2
+
:
+ A8Y + lb
----------e
Test Sanz 7.3
ml microbial
Total volume
4 200 = Soo + AOD 7 500
EE ----------
--e-- ee-- pe ----------------i---- ------ ------ ------ --------r -- eee -------- eee------ -- ee-- e
eem-- eee-- erome----------_etr-- e ------ ---- r ---- eer re
erree----------------------------------------------------------
eee--e ------------------------------------------ eeer--------------------------------------------eeeteee e
eeeer--------------------------------------------
-- ee------
e--e----e------------------------------r--------------
A
--
----
FE
--
eee eee re P----eeee
TT
rereee------------------------------------C0--38--76--
ENVIRONMENTAL LABORTORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST. #209
CALCULATION OF RESULTS
Test substance _EX=JOOZ Lot2327
30_- MINUTE EXPOSURE
ConcTeensttraStuibosntance(ng/L. CONTRO
0
Oxygen UpRtake Rate RtCoo2r0geCctedTM) | .bYRS 10.485 1 | .(a199 [0.194 |
RGIBRR
a
Y:
-
P43 42
+ RIenshpiibriattiioonniRante,(?) = q.
CONTRO
1 0.7139 Jo.7139 1 =
`Percent Difference in Control3s) 4 sk Ve
-
ECgy (95% Confidence Limits) mg/L
lQR BROS ConTceesnttraStuibsotnan(cneg/L)
QO
YeYe)
Oxygen UpRtake Rate RtaCofrree.cted! D + RIenshpi--ib--ri_at--tiioonniRnate(?)
|0.0|8 0.3 526 9| =
| 0.5503 [0.553 | )
1]
CONTRO
[r 0 | .7Tr 0 o e3 zi0 H 1[| --
Percent Difference in Controt2s.8T)n___ :
Ego (95% Confidence Linits)FPS cued BOLD rr--------------------------
(1) Temp. Corrections --RE (ng0,/L-nin.) of
8Disference =--l--2hx 100 (a + 2
@ Inhibitions, RC, + RC. 4. x _--
z
Tshheoutlwdobecownitrtohlinre15s%piorfateiaocnhraottehse
here: TR,o=bsOx=ygeSnamuppletakteempr.at,eC(~ng Oy2/L-min.)
6'=1.047, "x = T,obs - 20C
6 = Control
See py 4 for test Sohn. pry . o3877
o-a.3.0- P90
SamplePEDeSscDriipcthiiognrephenl
Aslerepce, SUPSIuNce
Lot D7-0b0-0
GABA
Lab Request No. F12+3
Environmental Lab --Work Sheet
ECD ACTIVATED SWDGE
RESPIRATION TNHIBITION TEST 1209
"9 --Po
Da-te: ~le-90
Analyt: SR
Stock and Test Solutions Preparation
STOCK SOLUTION: i a,
3,5-
2) [a OH
N
do. 30
L
ili- @ wade
Ade) 10K UpS0y under stirring, lropuise enti pit. pr
Diliited 4p ope. liter wih Milli-Q Water
Stocle Soln pH = 7.5
Zack Lo pd = 0.5 me 3,5-Dcf
Test Conc.
fant
10
mI st5o00cknlsoln
pu
lo
ry
MIrM)S0w0atmelr Wfleesdyn, perse5w0a0gmel milnomciuclruombialper $00 ml
A
+
+ Zoo
=
A74 +
lb
+
Zoo
=
Total volume
S00
Sa
-
C93878
ENVIONVINIAL LABORATORY
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST. 209
CALCULATION OF RESULTS
Test substance --3,.5=DichloaPlena 30. MINITE EXPOSUTE
IR2882,14
hee rtLhhd
4
P53 #3
Concentration (ng
CONTRO D CONTRO
R
to 20C
||0o..31,54)255
| 0.6985 |
| mp 2s5)s |
rr
rr1
1
1
TT
To7129 10 7134
Respiration Rate(' =
4g =
Percent Ditterence in Controts_ 1b Zo
co
Cg (95% Confidence Lints)wei 10 wd Il wduced YR 72ineib.
IER EEE
ConTceesnttrSautbisotnan(cneg/L)
CTR)
DO
Oxygen UpRtake Rate
RtoCo20rrCected + RIenshpiibriattiioonniRnate!'
| 0.6836 | 0.L529 | --
| 0.2109 |o.ge1d |
70
rr
1
1
1
CONTRO)
[ 0.70230 [| o.t7ty |
Percent Difference in Control(s)
28 Zn
.
=
.
() Teap. Corrections --LL (ng 0,/L-nin.)
o*
Re, + RC 3.
(2) Ishibition
X 100
-
Farin tve in) Where:
6'==1G.a0n4e7r,ol x= T,obs - 20C
(3) +Difference =L--1 dx 1
(= +=)
The tho control. respiration rat
should be within 15% of each ot
See ra 4 or
St Sein.prep. i C03879