Document Mod3myakpZMv0DdbdDBe73Bgy
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ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
TEST SUBSTANCE_____________________________________
Identity: Decafluoro(pentafluoroethyl)cyclohexanesulfonate, DEA salt; may be referred to as CS-2070. When dissolved in water may also be referred to or as CS-2151 or FC-90. (DEA salt of perfluoro(ethylcyclohexyl) sulfonate, [C2F5-CF(CF2CF2)2CFSO3-][H2N(CH2CH2OH)2+])
Remarks: The 3M production lot number was 3. The test sample is CS-2151, a mixture of CS-2070 in water (approximately 25% test substance and 75% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. The sample purity was not characterized.
METHOD:
Method: Standard Practice for Conducting Acute Toxicity Tests with
Fishes, Macroinvertebrates, and Amphibians (ASTM E729-80) Type: Acute static GLP: No
Year completed: 1981
Species: Daphnia magna
Supplier: In-house cultures. Originally obtained from Mogul-Ed,
Oshkosh, WI. Analytical monitoring: Temperature, pH, and dissolved oxygen.
Exposure period: 48-hours Test organism age: 24 hours old
Statistical methods: EC50values calculated using the probit program
and 3M TRAC Computer System. Test conditions:
Dilution water: Carbon-filtered well water.
Dilution water chemistry: provided for the end of 4th qtr, 1980.
Uclear when the toxicity study was conducted.
Hardness:
260 mg/L as CaCO3
Alkalinity:
221 mg/L as
pH 7.7
Conductivity:
340 mmhos/cm
Lighting: A daily photoperiod of 16 hours light and 8 hours dark with a
30-minute transition period was maintained throughout the testing period. Stock and test solution preparation: Not noted. Exposure vessels: 250 mL beakers containing 200 mL exposure
solution. Number of replicates: 2
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Number of daphnids per replicate: 20 Number of concentrations: five plus a blank control Water chemistry during the study: Not noted.
RESULTS___________________________________
Nominal concentrations: Blank control, 21.75, 28.75, 38.75, 52.5, and 70 mg/L.
Element value and 95% confidence interval: 48-hour EC50 = 74 (66 - 93) mg/L
Element value based on nominal concentrations.
Remarks: Testing was conducted on the test substance as described in the test substance remarks field. The values reported are based on nominal concentrations of CS-2151.
CONCLUSIONS____________________________________________
The test substance 48-hour EC50was determined to be 74 mg/L with a 95% confidence interval of 66 to 93 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY____________________________________________
Reliability: Klimisch ranking = 3. The results are from a summary report and there is no concentration-response data included. There is a lack of characterization of the test sample. Test solutions were not analyzed for test substance concentrations and confirmation of the amount of fluorochemical component in the solution.
REFERENCES______________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 6457S, 1981.
OTHER___________________________________________
Last changed: 4/1/01