Document Mo88Nwd374ymb65OG5J075px
AR226-0llY
LE-19
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity: Perfluorooctanesulfonate, Lithium salt; may also be referred to
a1,s1,P2F,2O,S3,3Li,4s,a4l,t,5,F5C,-69,64,,7,o7r,F8,C8-,984--hXe.pta(d1e-cOacftluaonreos-u,lflointihicuamcsiadl,t, CAS #29457-72-5)
Remarks: The test sample is a mixture of the test substance in water
(approximately 24.5% test substance and 75.5% water). No calculations
were made to adjust for the actual concentration of the test substance in the test sample.
METHOD
Method: OECD 209
Test type: Static acute
GLP: No
Year Completed: 1994 Analytical monitoring: pH and DO concentrations.
Statistical methods: Not performed
Test organism source: Activated sludge mixedliquorcollected from the
Metropolitan Wastewater Treatment Plant, St. Paul, Minnesota.
Test conditions Dilution water: Millipore Milli-Q water Synthetic Sewage: Per OECD method
Reference and test solution preparation: A stock solutionof the reference substance, 3,5-dichlorophenol, was prepared per the
OECD method. The pH of the reference solution was measured to be 7.4. The test solutions were created by direct addition of the
test sample; final concentration of 1000 mg/L. Initial pH of the two 1000 mg/L test solutions was 7.1 and 7.2. Test vessels: One liter glass fleakers.
Number of concentrations: 1 (done in duplicate) plus 1 reference
control and 2 Blank controls.
Temperature: 14.7-16.8C
Total Suspended Solids and pHfor sludge on day of testing: Total Suspended Solids = 4.9 g/L (mixed liquor). Final test solution mixed liquor suspended solids = 1.92 g/L. pH of the activated
sludge mixed liquor gave an initial readingof 6.4 and a final reading
of 6.7.
Element Basis: Respiration inhibition as determined by oxygen
`consumption.
001554
Method Remarks: Dissolved oxygen in the test solutions was quite low during the 30-minute exposure portion of the test. The technician realized the test reactors were not being sufficiently aerated after the 30-minute readings and adjusted theair flow. Because the 3-hour data is the most commonly used data, it was decided not to retest due to this occurrence. Test temperatures were not controlled during the test. The respiration rates were normalized to 20C for the calculations. RESULTS Nominal concentrations: Two Blank controls, one reference substance (10 mg/L 3,5-DCP), two 1000 mg/L test material solutions. Analytical Methodology: Analysis of DO concentrations in all test solutions were performed using a dissolved oxygen meter. Control response: Satisfactory Remarks: Values reported are for the test sample. No calculations were made to adjust for the concentration of the test substance in the test sample. CONCLUSIONS The test sample containing 24.5% Perfluorooctanesulfonate, Lithium salt exhibited a maximum inhibitory effect of 35% upon respiration at a nominal test substance concentration of 1000 mg/L at 3 hours exposure. The 3-hour EC50 (respiration inhibition) is >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 2. This study lacks information on the sample purity, and the test was conducted at temperatures below those recommended by the method. Oxygen concentrations also were too low to allow an adequate measurement of oxygen uptake rate following the 30minute exposure portion. These 30-minute results were considered invalid for the study. It was concluded that, since the 3-hour results show acceptable toxicity, the 30-minute results aimost certainly would have shown the same. REFERENCES
001555
This study was conducted by the 3M Company, Environmental
Laboratory, Lab Request number M1018, 2/22/94.
OTHER Last changed: 5/3/00
001556
tH. .
EVALUATION OF FC-94-X BY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
Results
The inhibitory effect of FC-94-X, the lithium salt of perfluorooctanesulfonate, on activated sludge respiration was evaluated using OECD Test # 209. After calculated temperature corrections, measurements following a 3-hours
exposure to FX-94-X at 1000 mg/L, showed activated sludge oxygen uptake
rates were inhibited by 34%. The reference substance, 3,5-dichlorophenol, at 10 mg/L caused an inhibitionof56%.
Discussion
Though this study had deficits, the analyst considers that the results are sufficient to assess thepotential for wastewater treatment system inhibition by FC-94-X.
One study deficit was that oxygen concentrations were too low to allow an
adequate measurement of oxygen uptake rate following the 30-minute
exposure portion of the OECD #209 study. These 30-minute results are thus invalid and should be ignored. The 30-minute results are not critical to the
usefulness of the study, however, because inhibition is almost always greater following 3-hours of exposure. If the 3-hour results show acceptable toxicity,
the 30-minute results almost certainly would have shown the same.
A second deficit is that temperature control was not used. Measured test temperatures were lower than that recommendedbythe method by as much as 5.5 C. However, all tests were conducted within 1.5 degrees of each other. Also, prior to comparison of the oxygen uptake rates, calculations were made to adjust the rates to those likely to have occurred at 20 C. The adjustment used was the rule of thumb that metabolic rates of mesophilic microbial populations typically double for each 10 C increase in temperature.
July 22, 1998
001557
INTERNAL CORRESPONDENCE/3M
TO: ERIC A REINER
FROM: SUSAN A. BEACH
SUBJECT: Review of OECD 209 and BOD/COD Test Results for FC-84-X
DATE: July 8, 1998
(1 OhEavCeDr2e0v9i)ewaendd thhaevreesaulftoswfcroommmtehnettse.st of FC-94-X on the inhibition of activated sludge respiration inhibition 1. ATSleutsfhtiociultgowhnattsyhaaeeddreiaqstuseaodltveaedaeoxftyh&getehGnoouir-rmstiheenxputoesrsteuarsdeoi.lnugtTsoh,neasntwdeacashdijqcuuiisatteneldroehwaeldiuzareiidnfghloetwh.teesB3te0cr-eamaiucnstueotretshewexahprooesuunorrtedbpairtnaigontohfethe
`most commonly used data, it was decided not to re-test due to this occurrence.
2. Ath time ths test was run, thelaboratory did not have procedur n place for controling the temperature hdreuisrspiiwnrgaasteixotpnaossrtauetrdees.inwTFeerhbeernutoaermmpymeairinazMteiudnrentoeofs2ot0thaettfeorsstt hnreeoaccstauolprcrsiifalstuicontgnusta.theadwtihtehttehsetetmepmepreartautruarewiansthseo hloowo.d.ThBoecause
3. Taahueasloifntwoarrtehuesteedmpteorpaturre atochesddtaetduarreescdpuicrtaeitoinonsrhaeleests(iRda)noihavfe anoieuandseycrwsaiauyndto.de1pictasexfpololnoewnst:s,sothe
Ra-Redused020C= _A_
Tho md E50
oF
Ao cote Cn A1=6!
.
WhXe=re(0obs=er1v.e0d7)2- 20C
fvieeet= rler y 7
4. Even though the 30-minute data in question and the test was conducted a lowerthan noma temperatures, tishinghenidibfafiticoaannatwrwaeshussneofouulsnifanotgr 1tth0hie0s0ipnmtregroLpc.reetWadtauiodrngueeeonfoatrhatehlteyoxndihcoiatnryootonftcoFvnCas-ri9id4ea-riXiiynthooifabicttthiieovnamtleeotdshssotlduh.dagne.orAenquaavler0ag1e5o%f f3o4b%o
As for the BOD/COD testing, we have been unable to locate the dataatthis time.
Pleasesee me if youhaveanyquestions.
Lak
`Susan A. Beach
001558
-
wR Mii
Date 2A
ENVIRONMENTAL LABORATORY
Analyst
R
Page _L of
ACTIVATEDSLUDGE MIXED LIQUOR
Type Test: OECD ACTIVATED SLUDGE RESPIRATION INHIBITION, # 209
ACTIVATEDSLUDGEMIXED LIQUOR
oate Obtained 2(23154.
Date Used 2223
Mixed Liquor Suspended Solids (MLSS)__U:%l gi. Adusted'to _---- gL
MLSS in final test solution _| TX" gn
inital pH (4 (1st tost soln. prepared)
Final pH (oF (last soln. prepared)
Comments
TESTSUBSTANCE
Sample Description _FC--~Q4 =X"
Initial pH of highest conc. tested (0CDMg/e) = 1.22
Comments
Adjusted to pH
---
te
Sample Description 3,5 = IC. RLORCT Snennl
Initial pH of stock solution (S00_ mg) = 2.4
"Adjusted to pH _--_
comments 00000000000
EXPOSURE CONDITIONS
Test Duration
2 HooesS
Test Temperature Range: 14.3 =I,
Comments
-_---- 001559
ENVIRONMENTAL LABORATORY
rn"RyMaAoDRsd
OECD ACTIVATETDESSTLUSDOGLEUTRIEOSNPSIRPARTEIPOANRAINTHIIOBNITIONTEST#209
Test Substance: EC --QU-x 000000000 Sample Daseription: Becianisn-yewad Ligod Analyst: BLD
pate: 22294
Test Cone. `pemrgSa0d0demdl
mee --
00
SO
1000
S00
m_LgMeQr!S)owoamtelr
284 289 29
fmeLesdycne. rsSeOwOagMeL
Je 16 1G
ocmuLummicoroebiarlSomol
200 200
m Total VL olume
Son TD
Tock tfajos fo CouC: Tar Soln_ulu
1006
Ios
[PN pel
2.2
RAY
(1) Millipore MillaR high purity water
8
-
001560
ENVIRONMENTAL LABORATORY
Ry BokY
.
OECD ACTIVATED SLUDGE RESPIRATION INHIEITION TEST #208
RAW DATA
:
Test Substance: _EC-AU-X
Source of Activated Siudge Mixed Liquor: Li
Oate Received: R[22jay nays: 29)
dee Teatrnent Plog ST.Bwt san.
Date Used: 223[5
39-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATICNS, moll
Test Subsancs
TIME MINUTES
Temp
Cancentration (ic 10.5 | 1.0 [1s 20 [25 [3.0] 35
<
EKCoNTRLT
0 7600
m=
[eeea el abod] holesJeeJeolsalslaunl[s4es lua ls lec
pr 1 TT 1 T 1 1 7 1 1
IZZ3NFLS 12 To[0306 =[=[= [= JTe<
[1 1 1 1117 1 TT
a 08lntl T=o1g=l 1-= T-"1="1""Ted
TT
Trrr
I
80
170 68 les 1.2159 Ise ls. Ist {isa
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mg.
:
Test Substance
TIME - MINUTES
Temp
Cancenration (myc [0.5 [1.0 [1.5
2.5 [3.0 [3.5
c
EKGoNTRGLT
Ie Toe SKeaNTRGLT
19 |e2a[21 [ed [esos ole [sas [sil]
T TT 1 1 1 1 T TT 1
LL [63[60[SASF 56153 [SA199 197195 [16.0
1
| 1 11 1 1 1 1 1
153 [S199197195 [43[91 139 [RF155
13.]cs
1
1 rr1 T
I 4 HS AI AN
A NdNela
S2HH ooff Nacotniavsattedcosnlucdgaenramdiexnedelsitgueodr,: IInniiatiUal_1S{
Test temperature: _14.7- 1b,3C
i Final lp.
001561
ENVIROMENTALLABORATORY C(AOCECUDAACTTIOVNATGEFDRSLEUSDTGESRESPIRATION INHIBITIONTEST#209
Tost Substance: ECtx
Anaya:Bt sone Exponire
Disoved Oxygen Concntrtns, mg
Lr wisn RDayte: d2/o22d/94 4333gB
rTTT
1 1 1 1 1 1T T= 1T 1 1
1
[osool23 v7 [vs[72[70Tos[os| I~1117768|o4ras[os0zs[ 31 |
[ir msoslr12[r TroT s[+rosT r[0r8r r [ rT rr rrr rr | es|rTrrodou11o[oawwr] a1]
ET rT
[c B2[o 80 i 77|7(104.7[7768|s6b5(]356% 252 61000|m56o]l| 5461] 162 |ose06 T0ac6G10.0005636]|
Stour Exponre
Dissoived Oxygen Concntrtions, mg
[mgr os]10115T20125713035]40]45]507155] ume] A (1) Tha)[%ion is]
[ai] IE iE ii il
1]
il IIE
iF
=
|
[81[70[7674[71/68|6563]60|67]54] 1467[1o6] se[ s osorOe]|
Tr Tr TT 1 1
1
[oo00/ 66[63 60[60[57 |65[63 |61|40[47|45] 160|0015 [05170] 35 |
1 1 1 1 1 1 T 1 17 T 1T 1 T 1
[oeco] 53 87140[47[as[43[47[503573531360]| 0.4000 06316] 33 |
C Idi roni (02.9 6 6 @6% oto mol AyTFBar ns6 B0.7s 087 [exz]7417.1]6o 6562]6067|6462]40]46] 140|06630[0.7807] |
Y@R r-- ence Pn Xnan o he n ACH Se vane Pus
tt t5s
ENVIRNOMENTAL LABORATORY
RPAIaOI\EoSrMsIYg
OECD ACTIVATTEEDSTSLSUODLGUETIROENSSPIPRRAETPIAORNATIINOHINBITIONTEST #208
Test Substance: B,5=INCH cenPrac Reference Substance
Sample Description: Red Lcioe\ ker lo OFAODA
Analyst: "RID
pate: 22074
Stock Solution Preparation:
Sen,
me JL
Ea
\ \oL = 0S og
ANDEoSlued~ 30COdYS MG"DwCoPtee
Of 0X] Ajaon
ADDILDIETDED 0iAr )_W3S0yTir,ed DeoNMmOsMEGUNDuEn RSte@ilnantmapaPPT
a EE E e
--
TestConc. permSL0ad0dmedl
KC IE
10
10
mLpMeQ(r"S)OOwmatler
274
mfLeesydnp.esreSw0aOgmeL
[5 lo
lccmLumuimcrooebiraslooml Tom talVol lume
R00
Q
306
00
r-- e r ------------i-- r --s i---- ---------- ---- ------ --i -- ---------- ---------- ---- cc--r er--m--------e ------------e ------------s ie--ime--merr ----e r---- ----------
(1) Millipore Mill-QR high purity water 001563
tS :
ENVIRONMENTAL LABORATORY
Ty 203
OECD ACTIVATED SLUDGE RESFIRATION INHIBITION TEST #202
RAW DATA
Test Substance: 3.5
:
Source of Activated Siudge Mixed Liquor:
Date Received: X[d2(94
Analysto : y
CE SobStin
TectwentPlea ST7. ;
Date Used: _2/22/54
30-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mg.
-
Test Substance
TIME-MINUTES
Temp
Concentration (hl)C|
2.5 | 3.035
4.5
c
EKCONTRCLI
I)
EcaNTReLE
sulso [ur [us [uo [roles
Be1g [BR 18] [1 792.7[2. [7.5 2171.003 [eF/%F Fr rrrT
en rrrrr
rrTT Tr
r gol11 blu los lr r esleT alss1 leeT lsaT lsn1lisa
3HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mol
Test Substance
TIME - MINUTES
Concentration (#5/L )0.5
1.5
2.5 [3.0]3.5 4.045
Temp
c
Aymaraer apt mnie EKCoNTRCLT 10
Seawal=l
aHof tedsi
Test temperature:
1a[70 [2 bu Jlegecleslealos llos) [ia
#2 (3) 18.0 [7.9 118 0.917.5 Nd17.3 17.2[1.0]
1 T 71T 1 1T 1T 7 7 7
1
Tr
1
1
1 1 TT 17 1 1 7
1|
1T1
Dio 1bgr burr lea1 feol1 sz s1 alsa1 lea1 [dels
ixed liquor: Initial___Ld
i
+
_14, $=1.6 C
001564
`ECONAEVLCICRDUAOACNTTMIIEVONANTTOAEFLDRLSEALSBUUODLRGTAESTROERSPYIRATIONNHBITIONTEST 208 oatSubstance: 2.5DichlamohenoRserenceSubstance nly:Bt
ouL:r i2022n004 1 Py3os3 8 g3
s0Minute Exponure
Disa Onygan Concontations, mg
Elm a
Er rr a
[E rokete{{r 7o.s0T1r o60T[ o [6 87[4 75076 s7.a7 l[0 7680|]] 7 |a 72r e |7[0|4 6a8e3|]t] veare4[|007se0] as0[oos20s7o]eO5s8 ]|
r r rr rr r r r r r r r re
Be %eGTTocoonT |or7(701710a.7|T 7316r 6E|5r e0s(6%ozOF:36100m|r a6ll8 salerr | 6s[oesF] aecs6o:l00.85]35
Stour Exposure
Disshed Orpen Conconsaions, mg
[FE c7oIi{F 7f6(74] 7.1 60 0s[63]00 67]F alas14]7 |osse enleose &or) a|l
[r rer pt r prer pa tepa IspA IEr 1S fea fess) 58
[r edizt c7oan1o7(lO1F 602.3 |66|62]66500(155%5O7F]51a0 mr |a6l.2|40 |46| vas |osoF[aocmne(5r1-- 0 0.79]-- 87
@heeonnnaat nrPuit rte cere
c=oma cn ee oescmsn
2,1 00%ahtencceePanoRIC 1crt mBwn 68h hh) l[e10esec