Document Mm5168DpdJwGOgqbBzE3Nyzj
AR 226 _ O743
ACUTE TOXICITY TO FISH (MUMMICHOG)
TEST SUBS" ANCE
Identity:
A mixturecontainingperfluorooctanesulfonate; may also be referred to as PFOS, FC-95, oras a component of FC-203C a1,n1d,2L,-25,434,03.,4,(41,-5O,c5t,a6n6e,s7u,l7f,o8,n8i,c8a-chiedp,tadecafluoro-, potassium salt, CAS # 2795-39-3)
Remarks:
sample is
The 3M production lot number
FC-203C and is referred to by the
was not noted. The test
testing laboratory as "81-1/8".
Current information indicates it is a mixture of 1.47% test substance, 60%
`hwyadterro,xy30fo%amdeiret,h1yl0e%nepgollyycooxlybeutthyylleetnheer`,mo3n%ooscotydlipuhmenoycltyeltshuelrf,at0e.,132%%
sodium lauryl sulfate, and 0.05% benzotriazol.
Ttehset fsoulblsotwainngcesiunmwmaaterry saoplpultiieosnt`owitthheitnecsotmspalmeptelleyacshaaramcitxetruirzeeodfthe concentrationof impurities. Data`may not accurately relatetoxicity of the test sample with that of the fluorochemical component.
METHOD:
Method: EG&G, Bionomics protocol entitled "Protocol for Flow-through Toxicity Tests with Fishes," January, 1980.
Test type: Flow-through acute
GLP: No
`Year Completed: 1981
SSuppepcliieesr:: FCuonmdmuelrucshieatlersoucplpiliteurs Analytical monitoring: Temperature,
pH,
DO,
and
salinity
Exposure period: 96-hours Statistical methods: LCso values
calculated
using
the
computer
program
(created by Stephan, 1978) based on the `moving average angle analysis
(24-, 48-, and 72-hour LCsos), Of binomial `probability (96-hourLCso).
Test fish age: Adult. Length and weight:
Length = 31 = 37 mm with a mean of 35 mm
Weight = 0.43 --0.809 with a meanof 0.63 9
Loading: 0.13 g/L
Pretreatment: Not noted.
Test conditions:
Dilution water:
Natural,
filtered
seawater
from
Big
Lagoon,
a
Gulf
of Mexico estuary, Florida.
Dilution water chemistry:
Salinity: 28 -- 29 parts per thousand
001022
Lighting: Fluorescent lights provided a daily photoperiod of 12 hours light
and 12 hours dark with an intensity of 1,100 lux incidenttothe water
surface.
n preparation: A primary stock solution of
Stock andtestsolutio 523,000 mg/L was prepared
by
adding
523
gramsof test
sample
to
a
1-L.
volumetric flask and bringing to volume with deionized water. All test
solutions were prepared by a diluter delivering five nominal `concentrations
oFfltohwe-ttehsrtosuagmhprlaetea:ndApcpornotrxoilmawtaetelry.3 aquarium volume replacements per
24 hour period. `Stability ofthe
test
chemical
solution:
Notnoted.
Exposure vessels: 30 x 30 x 91 cm glass tanks. containing ~ 50 liters of
solution volume.
Number of replicates: two
Number of fish per replicate: 10
-
WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e 5stpulduys:a blank control
pH range (0-96 hours):
7.8
7.6
8.0
-8.0
((c2o0n0tr0olm)g/L)
Temperature range (0-36 hours):
Dissol2v2ed-- o2x4ygCe(nforr aalnlgaequ(a0r-i9a6) hours):
6.3 -- 7.1 mg/L (control)
3.87.1 mg/L (2000 mg/L)
Salinity range (0-96 hours):
28 -- 29 parts per thousand (for all aquaria)
RESULTS
Nominal concentrations: Bk control, 125, 250, 500, 1000, and 2000
[onJom PRwRr mg/L
Percent mortality (20 fish per concentration)
Nominal
[Comm ms
| |o 0 | 0 _ 1 | 0 0T o [0 o1
2s [so
|o 0 | oT |o oo o T7T 0 0| |
To2o 00 [ | 0o [| 5 | _ [ o es T | o o 0J
Element value:
24-hour LCso = 1,500 (1,400 1,600) mg/L
4782--hhoouurr LLCCssoo == 11,,550000 ((11,,440000 11,,660000)) mmgg//LL.
96-hour LCso = 1,400 (1,000 --2,000) mg/L.
001023
Element values based on nominal concentrations.
dReesmcarrikbsed: iTnetshteintgeswtassubcsotnadnucceterdemoanrkthsefimelidx.tuTrheeofvtahltueesrtepsourtbesdtaanpcpleiaess
1o that mixture and not the fluorochemical proportion alone.
CONCLUSIONS
Tbhee1,F4C0-02m0g3iCL9w6i-thhoaur9L5C%socofonrfiFduenndcueluinstehrevtaelroofci1t,u0s0w0atso 2d,e0t0e0rmmign/Le.d to
`PSauublm,iMtitnern:es3oMtaC,o5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St.
DATA QUALITY
_
tReesltiianbgi.litHyo:weKviemri,scshamrpalnkeipnugr2t.y Twhaes sntoutdpyrompeeertlsy cahlacrraicttereiraizfeordqaunaldittyhe psrtoupdoyrtliaocnksinantahleytsioclaulticono.nfirmationofthe amount of fluorochemical
REFERENCES
TLhaibsorsattuodryywoafsPecnosnadcuocltae,dFbLyaEt Gth&eGrBeioqnouromeficstshteMa3riMneCoRmepsaenayr,ch3M Lab Request number 7038, 1981 OTHER Last changed: 6126/00
001024
EAT# S120
LR No. 703%
FC-203C Ascoultuetiotnoxi(c8i1t-y1/8o)f atosuthrefamcutmamnitchog
Fundulus heteroclitus in a flow-
through system
Toxicity Test Report
Submitted to
Minnesota Mining & Manufacturing Company
Environmental Engineering and Pollution Control
Environmental Laboratory
St. Paul, Minnesota 55133
:
\
Project Number R36 Report Number BP-81-10-163
EG&G Bionomics. Marine Research
Laboratory
10307 Gulf Beach Highway
Pensacola, Florida 32507
October 1981
001025
MarineEGR&eGseBairocnhomLiacbsoratory 10307 Gulf Beach Highway Pensacola, Florida 32507
TOXICITY TEST SUMMARY SHEET
Client: Minnesota Mining and Manufacturing Company
Client Contact or Principal Investigator: Mr. Eric Reiner
Report date & number: October 1981; BP-81-10-163
Bionomics' project number: R36
Study Director: Mr. G. Scott
Pest material: Surfactant solution 81-1/8
.
-
Ward
Description: Deep orange-yellow liquid
Date material received: 12 August 1981
-
Date of definitive test: 5-9 October 1981
Test condition: Flow-through, 96-hour duration, 23:l degreesCelcius
Test procedure:
BTMeRsLtstweistthprFoitsohceosl,," "dPartoetdoco2l9
for Flow-through January 1980.
Toxicity
Pest animal: Mummichog; Fundulus heteroclitus
:
Source and age: Purchased from a commercial supplier, adults
Seawater: Nsaatluirnailt,y filtered, from Big Lagoon, 28-29 parts per thousand
Solvent/carrier: none
Nominal test 7 concentrations: ..p1e2r5, l2i5t0e,r 5(0m0g,/%)1,000 and 2,000 : milligrams
Effect criterion: Mortality
96 -hour LC50: 1,400 mg/% 95% confidence limits: 1,000 - 2,000 mg/%
001026
1
A flow-through marine toxicity test was conducted at EG&G
Bionomics Marine Research Laboratory (BMRL), Pensacola, Florida, to
determine the acute effect of a surfactant solution (81-1/8) on the
mummichog Fundufus heteroclitus. The criterion for effect was death.
Results of the test are reported as 24-, 48-, 72-, and 96-hour
1050 values (the concentrations of test material estimated to be'
lethal to 50% of the test animals at the specified times of exposure) .
All data related to this study are stored at BMRL.
MATERIALS AND METHODS
Test material
The test material was received at BMRL on 12 August 1981 in one
9.5 liter (%) cubetainer labeled "3M SAMPLE 81-1/8."
a deep orange-yellow liquid, was tested as received.
The test material,
All test concentrations are reported here as milligrams (mg) of
surfactant solution per of seawater or as parts per million (ppm).
Test animals
Mummichogs were purchased from a commercial supplier and maintained
at BMRL in flowing, natural seawater for 5 days. During the 96-hour
period immediately before the test, the salinity of the holding water
was 28-29 parts per thousand (/oo) and the temperature was 19-21 degree
Celsius (C). No mortality was observed during the entire holding
period.
The control fish were 31-37 millimeters (mm) standard length and
0.43-0.80 grams (g) wet weight; mean length and weight were 35 (s.D.2) mm and 0.63 (S.D.:0.10) g, respectively. The control fish appeared to
be representative of the test population.
001027
2
Test water Water used to maintain and test the fish was natural seawater which
was pumped from Big Lagoon, a Gulf of Mexico estuary adjacent to BURL. The pump intake was located about 80 meters (m) offshore at a depth of approximately 3 m. Water was pumped by a 316 stainless steel pump through hard polyvinylchloride (PVC) pipes, through sand-filled fiberglass filters, through charcoal-filled fiberglass filters, and through 10-micrometers (um) pore size polypropylene core filters into an elevated fiberglass reservoir. Water was chilled by Teflon heat exchangers and flowed by gravity into the laboratory. Test conditions
Methods for the 96-hour flow-through test were those described in BMRL's "Protocol for flow-through toxicity tests with fishes" dated 2 January 1980. The test was conducted in an intermittent-flow system by using a proportional diluter (Mount and Brungs, 1967) constructed to deliver 1 2/cycle/test aquarium at a dilution rate of 508. The average mumber of cycles was approximately 6.4/hour. This cycling rate provided approximately 3.0 volume additions in 24 hours.
The surfactant stock solution (523,000 mg/i) was prepared by weighing 523 g of surfactant solution in a 1-1 volumetric flask and bringing to volume with deionized water. The stock solution was placed in a 4-2 glass reservoir attached to a "dipping bird" (Mount and Brungs, 1967). A 14.9 milliliter volume of stock solution was
delivered into the mixing cell via the dipping bird during each
diluter cycle. A Teflon solenoid valve, controlled by float switches, regulated seawater flow into the water cells. Mixing of various volumes of uncontaminated dilution seawater from the water cells with
001028
3
various volumes of stock solution in the test material cells produced the five concentrations of test material which were distributed to the appropriate test chambers. Test chambers were 30 centimeters (cm) x 30-cm x 9l-cm glass aquaria which contained approximately 50% of
test solution or control seawater.
Light was provided by two 3.7-m fluorescent bulbs suspended 46 cm
above the test containers, providing approximately 1,100 lux incident
to the water surface. Photoperiod was 12 hours of light and 12 hours
of darkness.
-
Five concentrations of surfactant solution and a control were
maintained. Selection of surfactant solution concentrations were based
on the results of a 96-hour static test and a maximum test concentra-
tion established by verbal authorization from Mr. Eric Reiner of the
3m Company. The nominal concentrations were 125, 250, 500, 1,000, and
2,000 ppm. To begin the test, the diluter cycled until all test chambers were
filled with test solutions or control seawater. Ten fish were then
placed into each duplicate test container.
Survival of fish in all treatments was recorded at 24-hour
intervals. The dissolved oxygen concentration and pi were measured
in cach test container at 0 and 96 hours and in both controls, low,
middle, and high test solutions after 24, 48, and 72 hours of exposure.
The temperature and salinity were measured in both controls daily
throughout the test.
3
The test was conducted 5-9 October 1981.
001029
4
Statistical analyses Based on the results of the test with the surfactant solution,
24-, 48-, 72-, and 96-hour LC50's and 95% confidence limits were calculated. The computer program estimated LCS0 values by three statistical methods; moving average angle analysis, probit analysis, or binomial probability. The method selected was determined by the characteristics of the data, that is, the presence or absence of 0% and 100% mortality and the number of concentrations in which >03<100%
mortality occurred (Stephan, 1977). The moving average angle method
was used to calculate the 24-, 48-, and 72-hour LCS0's. The 96-hour LC50 was determined by the bionomial probability method.
RESULTS AND DISCUSSION After 24 hours of exposure to the surfactant solution, mortality ranged from 0% in surfactant concentrations <1,000 ppm to 90% in the 2,000 ppm test concentration. The 24 hour LCS0 was 1,500 ppm with 95% confidence limits of 1,400-1,600 ppm (Tables 1 and 2). After 96 hours of exposure, mortality of fish ranged from 0% in test concentrations <1,000 ppm to 100% in the 2,000 ppm test concentra: tion (Table 1). The 96-hour LC50 was 1,400 ppm with 95% confidence limits of 1,000-2,000 ppm (Table 2). Physical or behavorial
abnormalities were observed only in the 2,000 ppm test concentration.
After 24 hours of exposure, the 2 surviving fish in 2,000 ppm were observed to be lethargic and remained so until their death.
Salinity during the test ranged from 28-29 /oo. The temprature ranged from 22-249C; mean was 23#1C. The dissolved oxygen concentration remained >30% of saturation in all test containers throughout
001030
5
the exposure. The pi was 7.5-8.0 in all test containers throughout
the exposure (Appendix A).
Co
001031
6 REFERENCES EG&G Bionomics Marine Research Laboratory. 1980. Protocol for Flow-Through Toxicity Test with Fishes. 2 January 1981.
5 pp.
Mount, D.I. and W. A. Brungs. 1967. A simplified dosing apparatus for fish toxicological studies. Water Research, 1:21-29.
Stephan, C.E. 1977. Methods for calculating an LC50. ASTM, Aquatic Toxicology and Hazard Evaluation, .ASTM STP 634. F.L. Mayer and J.L. Hamelink, eds. pp. 65-84.
001032
7
TABLE 1. MaToerssttuarlcfioatncycteanonfttramstuoimlomunitscihoonagrse(8(g1Fi-unv1d/eu8nf)usasihnenteofrmloiocnwtiiatnlugs)cnoeanxtcpueornsatelrdatstieooanwsateorf. whole material.
ConcNeonmtirnaatlion Control 125 250
500
1,000 2,000
SE
(mg/%:ppm)
Eom 0 0 0
0
o 90
MortRaetlity o 0 0
To
0 95
72(3)how o 0 0
o 95
96 How 0 o o
o
100
--------
001033
TABLE 2.
8 CtaolcaulsautrefdacLtCa5n0t'ssolfuotriomnumm(i8c1h-o1/g8s) (iFunndfulfuoswihnegternoactfiutruasl) seexapwoasteedr. Twheosltecmoantceernitarla.tions are given as nominal concentrations of
Hour
C50 (mg/%; ppm)
95% co(nmfgi/2d;epnpcme) limits
24
1,500
1,400-1,600
8
1,500
1,400-1,600
:
72
1,500
1,400-1,600
96
1,400
1,000-2,000
001034
PREPARED BY: AUDITED BY:
6. scott ward SGtu.dySbeiscecAtorDed.
5 20 OBamtae n \agl
Alan 6. miller
Yb, nt,
Guattey assixence Galt
19Jadbaltee 5r8/)
Raw data avait: Oley /9/
Preliminary report avait: s Ody /%/
Final report await: _J12idbso y
REVIEWED AND APPROVED BY:
Peter J. Shuba, Ph.D.
Pas
TecinioaT
1
Shee
Foordinater
22 OebouDlatae y
001035
.
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APPENDIX A
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SRRCT: ACUTE TONIEITY OATA SHEET
CLIENT: 3M Company
"PROJECT NO: R36
SPECIES:
Fundulus heteroclitus
TESTMATERIAL: Surfactant Solution (81-1/8
J wistoRy
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