Document MMO1ZQZ185JaGJ77paO3x80ma
AR226-03%9
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G..DAPHNIA)
TEST SUBSTANCE
Identity:
Perfluorodecanesulfonate, ammonium salt; may also be referred
to as PFDS, FC-121-X, or 3M Sample E2112 and E2566-1 a-
Decanesulfonic acid,
1,1.2,23,34,4556,6,7,7.8,8,9,9,10,10,10-heneicosafluoro-,
ammonium salt, CAS # 67906-42-7)
Remarks: Test sample is a mixture of the test substance in water and 2propanol, 1-(1,1-dimethylethoxy). This mixture contains approximately 22-
26% test substance, 33-37% propanol, 1-(1 \1-dimethylethoxy), 0.5-1.5% perfluorononanesulfonate, ammonium salt and 38-42% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. Test `sample was from 3M production lot 1
METHOD
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_
MEextahmoidn:atEioPnAo6f6W0a/t3e-r75a-n0d09WaasntdeAwaPtHeAr (1980) Standard Methods for the
Test type: Static acute
GLP: Yes
Year Completed: 1988
Species: Daphnia magna
Analytical monitoring: pH, dissolved oxygen, temperature. Test substance concentration was not monitored. Statistical methods: EC; values calculated using Binomial method Test daphnid source: ABC Laboratories In-house cultures, Columbia, MO
Testcondions Test daphnid age at study initiation: < 24-hours
Dilution water: Well water
Dilution water chemistry (from a representative sample):
hardness:
246 mg/L.
alkalinity
178 mg/L
pH:
8.0
Stock and test solutions preparation: Not given. A cloudy
appearance and surface foam present at preparation in the 560 and
1000 mg/L concentrations was not noted at the 24- and 48-hour
observation periods.
Exposure vessels: 250 mL glass beakers containing 200 mL of
test solution.
0001.00
Number of replicates: 2
Number Number
of of
daphnids per replicate: 10 concentrations: five plus a
negative
control
Water
chemistry Dissolved
during the study: oxygen range (0-48
hours)
87 -9.2 mg (control exposure)
pH
86-9.2mg/L (1000 range (0-48 hours).
mg/L
exposure)
82-8.2 82-84
(control exposure) (1000 mg/L exposure)
Test temperature range (0 ~ 48 hours)
19 -20C
Element basis: mortality and sublethal effects
RESULTS
Nominal Element
vcaolnucee:ntrat2i4o-nhso:urBLkCsco;nt=ro2l0,010(01,001-8302,0)32m0g,/5L60,
1000
mg/L
48-hour 48-hour
LCso = NOEC
130 (100-180) mg/L (sublethal effects) = <
100
mg/L
dSaubplhentihdasl teefnfedcitnsgitnoctlhuedebdotqtuoimesocfetnecset vaensdsleolrs.
Statistical calculated
Eusvianlguatthieonb:inTomhiealLCme:thvoadl.ue
and
95%
confidence
interval
was
All element values based on nominal concentrations.
RMoermtaarliktsy:oVfaclounetsrorlesp:orNtoedneare for the test sample (mixture). No scaulbcsutlaatnicoensinwtehreetemsatdseamtpolea.djust for the concentration of the test
Cumulative percent mortality:
a TesotCtonc. | 2unous | annus
some 0| | w |ow | ww | ww| wm
[oo [0| w Co | io w | wo
anol
CONCLUSIONS
The test sample 48-hour ECso for Daphnia magna was determined to be 130 mg/L with a 95% confidence interval of 100 -- 180 mg/L.
Testing was conducted on the "as-sold" water (~22-26% subject matherial) The
mixture of the subject values reported apply
material to that
in
mixture and not directly to the subject material.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Klimisch ranking 2. This study, while well conducted, lacks
analytical data for:
test solutions; and
determination
determination
of
of
the
the
test substance
sample purity.
concentration in the
Because a mixture
was tested, care must be taken when interpreting the results. The solvent
tionxtihcietymioxfttuhree fclouuolrdochhaevmeiceanlhcaonmcpeodnoerntrestricted biological uptake and/or
REFERENCES
This study was conducted by ABC Laboratories, Columbia, MO on behalf of the 3M Company.
OTHER
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Submitted By: 7A2n0a0lytEiascealABBCioL-aCnheemtstry Laboratortes, Inc.
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Prepared by:
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TABLE OF CONTENTS
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Hardness (CaC0,) Alkalinity (Caco,) Conductivity Total Organic Carbon
MSuispsetndaed Solids Araente Soran Cadstun Cheostun Cobate
Concentration
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500 - 650 :mtos/ca 1.4 pp' (0.9 - 2.8 ppm)?
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