Document MMJ5YNEN3bw9y1N2DabOxOa5y

. AR 226 _ 0906 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: tMoixatsurPeFcOoSn,taiFnCi-n9g5,peorrflausoraococotmapnoesnuelnftonoafteF;C-m3a0y72a.lso(1-be referred Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-3072. Current information indicates it is a mixture of 1.67% test 5su%bsstoadncieu,m 3oc3i.y3l3s%ulfdaiteet,hy3l.e3n3e%glsyucloflonbuattyeld eatlhkeyrl,e2t9he.r23su%lfwaatteers,alt2s0,.48.36%0%urea, acrylic foamer, 1.67% triethanolamine, and 0.33% toly triazole. tTehset sfoulblsotwainncgesiunmwmaatrerysaoplpultiieosntwoitthheitnecsotmspalemtpelleyacshaaramcitxetruirzeeodfthe concentrationsof impurities. Data may not accurately relate toxicity of the test sample with that of the fluorochemical component. METHOD: Method: OECD Guideline 202 Type: Acute static YGeLaPr: cNomopleted: 1995 Species: Daphnia magna Supplier: In-house cultures. Originally obtained from U.S. EPA-ERL, Duluth, MN. Analytical monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: Not applicable due to study results. Test coDnidliuttiioonnsw:ater: Carbon-fitered well water (3M Well #2, St. Paul, MN). Dilutionwaterchemistry: Not given. Lighting: Not given. Stock and test solution preparation: A primary stock solution was prepared by dissolving 1000 mg of test substance concentrations were prepared by additionofstock in 1 L water. Exposure solution to each vessel. NExupmobseurreofverespsleilcsa:tes1:004mL vessels containing 50 mL test solution. : Number of daphnids per replicate: 5 Numberof concentrations: six plus a blank control Water chemistry during the study: pH range (0-48 hours): 8.3- 8.3 (control exposure) 002932 Temper8a.t1u8re.3ra(n1g0e00(0m4g8/Lhoeuxrpso)s:ure2)0.2 21.6 C Dissolved oxygen range (0-48 hours): 8.1 -- 8.4 mg/L (control exposure) 4.0 -8.1 mg/L (1000 mg/L exposure) RESULTS rrr Nominal concentrations: Blank control, 56, 100, 180, 320, 560, and 1000 mg/L. Element values: 48-hour ECs = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixtureof the test substance as tdheastcrmiibxetdurien atnhde tneostt tshuebsfltuaonrcoechreemmiacraklspfrioepldo.rtiTohneavlaolnue.es reported apply to CONCLUSIONS The test substance 48-hour ECs was determined to be >1000 mglL, the highest concentration tested. No mortality or sublethal effects were observed. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY tReesltiianbgi.litHyo:weKvieirmi,sschamrpalnkeipnugri=ty2.wTahsisnosttupdryopmeerleytschtahreacctrietreirziaedfoarnqduatlhietystudy sloalcuktsioann.alytical confirmation of the amount of fluorochemical proportion in REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number M3597, 1995. OTHER Last changed: 6/25/00. 002933 : BEST COPY AVAILABLE CnvmoNMENTALLABORATORY 4548 ACUTE TOHCITY TO DAPHNIA, APHNIA NAGA TeeTesT. sane x _stachenewaL TestsussTANGE: Foaoonss OLUTONWATER: CANBON.I ILTENED WELL WATER GMWELL 2ST. AUL uty TESTOAPHNID SOURCE: USEPA EAL OULU onteneceneo: sass oaMNOAGE, <zernoL nEowATES Wo.oF PwOSITESTChaueeR: TestsoLumoNvoLUNE: Som sizeor eT ouaueEn: 100m ote mnateo: a2na_iaie pon otemmATeo: 31s4e vEeSsTt `pcoincce. |TawootTudnHmlo Iow oo Svoeofonme]| bw o ce onnor 1o a S4 lCe1aE -h3f P20 5 f5y4 oao |lowa|n"me E le 8Jo saan; S alall nlea -dne =08 o 1_8 O ST J30. - Fo ) wn sr 1x BEES TC hi] 0 |o | ereYa Zomie Fwo |I 3% F=SS se =x=20 = = prpo) <5 2 Gh F0E eo 1-Ors 3 F Rafigd RTeObaE x iwpfordoog FaTe E FreO ooe lo nroor m11w3e a X -|eESAEST SER > woo |g froglEe pC auSld E [[r TeSmeET mamr irR oT r BrE AyNeaa EwrITE a MEv EE = | SBinE n nh mes sociuosb Ek RS A i a Ley a Fodeanr:eonhlL a4TRRRAEEREETA R ERYEE ESESE Radis SA RLEo dead a a aR h ee is al EE de all 002934