Document MM8JE3V2DXLO3waz91XJJmxeV

p. 1 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-011 2/17/2010 9:24:11 PM PFCkinetexLong.dam 35 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 130-PFDoA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFD0A IS Area 4402000 2990900 9639100 2911800 179780 9639100 292280 292280 292280 Analyte RT (Exp RT ) 1.20 (1.36) 1.95 (2.06) 2.00 (2.09) 3.02 (3.06) 3.83 (3.88) 3.83 (3.87) 4.48 (4.55) 5.03 (5.06) 5.47 (5.51) Analyte Area 72531 29905 327310 62698 65497 145900 41799 25222 13536 Calc. Cone (ng/mL) 0.353 0.126 0.333 0.200 0.316 0.149 0.244 0.051 0.079 Modified? No No No No No No No No No I cq 1 Page 1 of 170 122 P-2 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM IRC Summary I Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 SJ3C-PFOS PFDA 2 SJ3C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.19(1.36) 1.20(1.36) 1.97 (2.08) 0.00 (2.44) 2.00 (2.12) 1.99 (2.00) 3.01 (3.06) 3.01 (3.04) 3.83(3.88) 3.83(3.88) 3.83 (3.87) 4.48 (4.53) 4.48 (4.64) 5.00(5.13) 5.47 (5.56) Area 109810 4402000 10051 0 102740 9639100 9569 2911800 13652 179780 44124 12778 292280 6011 3984 Modified? Calc (Exp) MRM Ratio No 1.51 (2.16) Ratio Confirms ID No No 60.69 (43.19) No No 0.34(0.34) YES No 0.00 (0.00) No No 0.31 (0.32) YES No 29.45 (21.71) No No 0.15(0.14) YES No 46.44(17.53) No No 0.21 (0.19) YES No 2.74(1.60) No No 0.30(0.32) YES No 0.31 (0.28) YES No 6.99(3.71) No No 0.24 (0.34) No No 0.29 (0.29) YES Page 2 of 170 123 p.3 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Quantitative Peak Review PFOA 1 413.0/169.0 Da Baseline c Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline c Missed Wronn Peak K1001011-011 A JL PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Pnak 13C-PFNA 468.0/423.0 Da Baseline Missed Wrnnn Peak _______ 3^4 PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Pfak 3^3 13C-PFOS 503.0/80.0 Da o Baseline c Missed p W rong Peak PFUnA 1 563.0/519.0 Da c Baseline Missed ___r Wrnnn Peak___ 4.48 i PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Pnak 13C-PFUnA 565.0/270.0 Da Baseline Missed i Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak 1 PFDA 1 513.0/469.0 Da Baseline o Missed Wrnnn Peak PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da d Baseline Missed n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da Baseline c Missed n Wrnnn Pnak 1.99 _1 _ 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak___ 4^8 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak___ 5.47 PFTeA 1 713.0/669.0 Da Baseline Missed ___n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak Before After Page 3 of 170 124 C olum bia. A nalytical Services' Ion Ratio Confirmation p.4 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM K1001011-011 Before After Page 4 of 170 125 P-5 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-011 2/18/2010 3:44:48 AM PFCkinetexShort.dam 35 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 4936200 6228800 4224000 6228800 6228800 4224000 4655500 4655500 Analyte RT (Exp RT ) 1.23 (1.27) 3.54 (3.60) 4.20 (4.26) 5.25 (5.31) 6.17 (6.23) 6.26 (6.32) 6.26 (6.31) 7.57 (7.65) Analyte Area 62748 58471 43647 98948 25009 181990 4224000 4655500 Calc. Cone (ng/mL) 0.06 0.09 0.05 0.10 0.06 0.12 1.12 1.00 Modified? No No No No No No No No - z / ,i? / Page 89 o f 169 126 Service' p. 6 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.23 (1.27) 3.54 (3.60) 4.21 (4.26) 5.25(5.31) 6.17(6.22) 6.25 (6.32) 1.23 (1.26) 5.25 (5.30) Area 62748 58471 14931 10999 75442 68381 4936200 6228800 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.34(0.40) YES No 0.11 (0.10) YES No 3.02 (2.90) YES No 0.38(0.40) YES No 78.67 (13.20) No No 62.95(13.71) No Page 90 of 169 127 p. 7 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Quantitative Peak Review 1.23 >4 1 PFBA 1 213.0/169.0 Da i_ Baseline c Missed n Wrnnn Peak _I_ 13C-PFBA 217.0/172.0 Da Baseline c Missed ___ n-WmaaP-sak___ PFBS 1 1299.0/80.0 Da Baseline Missed n Wrnnn Pfiak PFHpA 1 363.0/169.0 Da Baseline c Missed ___n Wrong Peak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak 130-PFHxA 315.0/270.0 Da d Baseline Missed n Wrnnn Peak 7,f -,n*s 4<Vi K1001011-011 PFPeA 1 263.0/219.0 Da d Baseline c Missed n Wrnnn Peak I PFHxA 1 313.0/269.0 Da Baseline Missed i Wrnnn Peak 6^ 13C-PFHxA 315.0/270.0 Da Baseline u Missed n Wrono Peak___ 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Ppak S i `,0 b5 PFHxS 1 399.0/80.0 Da Baseline c Missed ___n Wronn Peak 2 CMS 180-PFHS 403.0/103.0 Da Baseline o Missed n Wrnnn Peak S _ 1 8 0 -P F H S 403.0/103.0 Da Baseline Missed ___ n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak___ S_13C-PFDA 515.0/470.0 Da Baseline Missed ___n W rnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrong Peak___ Page 91 of 169 128 iumMa Created with Analys?'R8eporter Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation rawt K1001011-011 ' v/wv-/_--^ 4. 1 ........ - PFBA2 213.0/169.0 Da u Baseline d Missed Wrong Peak PFPeA2 263.0/219.0 Da c Baseline c Missed o Wrong Peak PFBS2 299.0/99.0 Da Baseline c Missed Wrong Peak i -f 6.17 6. 5 '. !!Ll*f, i i iM ............ r ... S i 69 t>i .'it s` it n PFHpA2 363.0/319.0 Da d Baseline Missed c Wrong Peak PFHxS 2 399.0/99.0 Da Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da d Baseline a Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da d Baseline a Missed Wrong Peak PMiageisnelrtiile N/A Baseline Missed Wrong Peak Page 92 of 169 129 P-9 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SampiesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-012 2/17/2010 9:31:46 PM PFCkinetexLong.dam 36 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFD0A PFTeA 1 130-PFDoA IS Area 3936000 2665500 6965900 3291800 114850 6965900 197910 197910 197910 Analyte RT (Exp RT ) 1.24 (1.36) 2.02 (2.06) 2.04 (2.09) 3.06 (3.06) 3.84 (3.88) 3.85 (3.87) 4.50 (4.55) 5.02 (5.06) 5.44 (5.51) Analyte Area 77183 9569 163210 35834 24270 24210 10030 7470 4021 Calc. Cone (ng/mL) 0.415 0.067 0.234 0.097 0.170 0.050 0.091 N/A N/A Modified? No No No No No No No No No ( (O Page 5 of 170 130 kmbm aytcai Srvic@s" p. 10 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 SJ3C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFD0A PFTrA 2 PFTeA2 RT (Exp RT ) 1.26(1.36) 1.23(1.36) 2.04 (2.08) 0.00 (2.44) 2.04 (2.12) 2.04 (2.00) 3.03 (3.06) 3.03 (3.04) 3.87 (3.88) 3.84(3.88) 3.84 (3.87) 0.00 (4.53) 4.49 (4.64) 5.04 (5.13) 5.52(5.56) Area 118180 3936000 2270 0 54004 6965900 1631 3291800 5754 114850 8142 0 197910 481 209 Modified? Calc (Exp) MRM Ratio No 1.53 (2.16) Ratio Confirms ID No No 51.00 (43.19) YES No 0.24(0.34) No No 0.00 (0.00) No No 0.33(0.32) YES No 42.68 (21.71) No No 0.05 (0.14) No No 91.86(17.53) No No 0.24(0.19) YES No 4.73(1.60) No No 0.34 (0.32) YES No 0.00 (0.28) No No 19.73 (3.71) No No 0.06 (0.34) No No 0.05 (0.29) No Page 6 of 170 131 lurntbia p. 11 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Quantitative Peak Review I 1.23 1 PFOA 1 413.0/169.0 Da Baseline a Missed n Wronn Pnak 13C-PFOA 417.0/372.0 Da Baseline o Missed Wronn Peak 2.04 I_k_ K1001011-012 2.02 PFNA 1 463.0/419.0 Da Baseline Missed In Wronn Pnak 1 13C-PFNA 468.0/423.0 Da Baseline Missed Wrnnn Peak 3^ 3 PFOS 1 499.0/80.0 Da o Baseline c Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline c Missed n Wrnnn Paak PFDA 1 513.0/469.0 Da d Baseline Missed n Wrnnn Paak 13C-PFDA 515.0/470.0 Da Baseline d Missed n Wrnnn Paak v'A ; ' PFUnA 1 563.0/519.0 Da c Baseline o Missed /mno PoaK 13C-PFUnA 565.0/270.0 Da d Baseline o Missed n Wrnnn Peak PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak___ i : c 13C-PFOS 503.0/80.0 Da Baseline Missed ___n Wrnnn Peak___ 4.50 JL~ PFDoA 1 613.0/569.0 Da d Baseline Missed n Wrnnn Ppak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Pnak 5.02 PFTrA 1 663.0/619.0 Da Baseline Missed ___n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da d Baseline o Missed n Wrnnn Pnak___ 5.44 PFTeA 1 713.0/669.0 Da Baseline o Missed ___ r Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed ____n Wrnnn Peak Before After Page 7 of 170 132 p. 12 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Ion Ratio Confirmation 1.26 K1001011-012 10i J\ PFOA2 413.0/369.0 Da Baseline c Missed Wrong Peak Vs To TT To mm PFNA2 463.0/219.0 Da a Baseline g Missed o Wrong Peak PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak iSOflO * I 3 MWI PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak iwfi 100*1 PFUnA 2 563.0/269.0 Da u Baseline Missed Wrong Peak PFDS2 599.0/99.0 Da g Baseline Missed d Wrong Peak PFDoA2 613.0/169.0 Da Baseline c Missed Wrong Peak PFTrA2 663.0/169.0 Da a Baseline Missed Wrong Peak PFTeA 2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 8 of 170 133 /TC Columbia sJ Aaalflical Servicer p. 13 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-012 2/18/2010 3:54:23 AM PFCkinetexShort.dam 36 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 4296800 Analyte RT (Exp RT ) 1.25 (1.27) 5807500 3.54 (3.60) 3897700 4.20 (4.26) 5807500 5.25 (5.31) 5807500 6.17 (6.23) 3897700 6.26 (6.32) 4425000 6.26 (6.31) 4425000 '7.56 (7.65) Analyte Area 58253 67689 76854 116660 26744 181830 3897700 4425000 Calc. Cone (ng/mL) 0.07 0.11 0.08 0.14 0.07 0.13 1.09 1.00 Modified? No No No No No No No No Page 93 of 169 134 p. 14 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.25(1.27) 3.54 (3.60) 4.20 (4.26) 5.26(5.31) 6.17(6.22) 6.25(6.32) 1.23(1.26) 5.25(5.30) Area 58253 67689 23692 9299 80007 65157 4296800 5807500 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.31 (0.40) YES No 0.08(0.10) YES No 2.99(2.90) YES No 0.36(0.40) YES No 73.76 (13.20) No No 49.78(13.71) No Page 94 o f 169 135 bia im Quantitative Peak Review I l1.23 PFBA 1 1213.0/169.0 Da c Baseline u Missed n Wronn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wronn Peak PFBS 1 1299.0/80.0 Da d Baseline Missed n Wrnnn Pnak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Ppak PFHpA 1 363.0/169.0 Da Baseline c Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak p. 15 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM K1001011-012 I I5.25 PFPeA 1 263.0/219.0 Da Baseline g Missed n Wrnnn Peak I 130-PFHxA 1315.0/270.0 Da Baseline Missed ____n Wrnnn Peak----- 5.25 PFHxA 1 313.0/269.0 Da g Baseline c Missed r Wrnnn Peak 130-PFHxA 315.0/270.0 Da Baseline c Missed Wrnnn PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak S J 8 0 -P F H S 403.0/103.0 Da Baseline Missed ____n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da Baseline Missed ___ n W ronn Peak___ SJ3C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___ Page 95 of 169 136 p. 16 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation K1001011-012 ./'W'W.v, PFBA2 213.0/169.0 Da a Baseline c Missed c Wrong Peak 6.17 PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline Missed d Wrong Peak B0e5 IW. PFHxA 2 313.0/119.0 Da d Baseline Missed Wrong Peak This hiage is rid sraiable PFHpA2 363.0/319.0 Da Baseline Missed o Wrong Peak PFHxS2 399.0/99.0 Da d Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed a Wrong Peak Page 96 o f 169 137 Servie" P -17 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-013 2/17/2010 9:39:21 PM PFCkinetexLong.dam 37 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFD0A PFTeA 1 13C-PFDOA IS Area 3741200 2509200 7314300 3060500 121680 7314300 185240 185240 185240 Analyte RT (Exp RT ) 1.21 (1.36) 1.99 (2.06) 2.01 (2.09) 3.03 (3.06) 3.83 (3.88) 3.85 (3.87) 4.48 (4.55) 5.01 (5.06) 0.00 (5.51) Analyte Area 116960 26748 910900 15195 8965 11889 7627 7636 0 Calc. Cone (ng/mL) 0.645 0.132 1.180 0.040 0.038 0.034 0.076 N/A N/A Modified? No No No No No No No No No ,1? yyy i> 2 -" Page 9 of 170 138 p. 18 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM IRC Summary i Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 SJ3C-PFOS PFDA 2 SJ3C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.21 (1.36) 1.22(1.36) 1.96 (2.08) 0.00 (2.44) 2.01 (2.12) 2.02 (2.00) 3.00 (3.06) 3.04 (3.04) 0.00(3.88) 3.84 (3.88) 3.84 (3.87) 0.00 (4.53) 4.49 (4.64) 5.13(5.13) 5.49(5.56) Area 210520 3741200 5820 0 272290 7314300 1433 3060500 0 121680 5276 0 185240 326 243 Modified? Calc (Exp) MRM Ratio No 1.80 (2.16) Ratio Confirms IO YES No 31.99 (43.19) No No 0.22 (0.34) No No 0.00(0.00) No No 0.30(0.32) YES No 8.03 (21.71) No No 0.09(0.14) No No 201.41 (17.53) No No 0.00 (0.19) No No 13.57(1.60) No No 0.44 (0.32) No No 0.00 (0.28) No No 24.29 (3.71) No No 0.04(0.34) No No 0.00 (0.29) No Page 10 of 170 139 p. 19 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Quantitative Peak Review l _L1.22 PFOA 1 413.0/169.0 Da Baseline c Missed n Wronn Peak 13C-PFOA 417.0/372.0 Da o Baseline Missed ___ n Wronn Peak___ JL K1001011-013 . mo 'Z : M) .1 1.99 PFNA 1 463.0/419.0 Da o Baseline Missed n Wronn Peak 3,03 JL.Jl>*5 K*G 13C-PFNA 468.0/423.0 Da Baseline Missed ----- n Wronn Peak___ _______ 3 ^ .L ! " ;; PFOS 1 499.0/80.0 Da d Baseline Missed n Wrnnn Peak 3.83 13C-PFOS 503.0/80.0 Da Baseline Missed n Wronn Peak awuO ltiOufj PFDA 1 513.0/469.0 Da Baseline c Missed n Wrnnn Peak 3^5 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak PFUnA 1 563.0/519.0 Da o Baseline Missed ___ n Wrnnn Peak___ 4.48 PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFUnA 565.0/270.0 Da d Baseline Missed n Wrnnn Peak 4^9 PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak '.5000 ft UJO00 22 5000 5.01 to 45 54 55 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak______ 13C-PFOS 1503.0/80.0 Da Baseline Missed Wrnnn Peak 4d4 4.49 .<4 13C-PFDOA 615.0/169.0 Da GBaseline Miss n Wrnnn Peak PFTeA 1 713.0/669.0 Da d Baseline Missed ____n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak Before After 140 Page 11 o f 170 Ion Ratio Confirmation p. 20 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM K1001011 -013 Before After Page 12 of 170 141 Iiimbia p. 21 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-013 2/18/2010 4:03:55 AM PFCkinetexShort.dam 37 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 3983900 5186500 3590800 5186500 5186500 3590800 4287300 4287300 Analyte RT (ExpRT) 1.24 (1.27) 3.54 (3.60) 4.19 (4.26) 5.25 (5.31) 6.15 (6.23) 6.24 (6.32) 6.24 (6.31) 7.54 (7.65) Analyte Area 92475 208270 130910 274290 71390 766310 3590800 4287300 Calc. Cone (ng/mL) 0.16 0.41 0.12 0.46 0.33 0.63 1.04 1.00 Modified? No No No No No No No No J pi- Page 97 of 169 142 Services' p. 22 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.24(1.27) 3.54 (3.60) 4.19(4.26) 5.24 (5.31) 6.15(6.22) 6.24 (6.32) 1.24(1.26) 5.24(5.30) Area 92475 208270 49564 24822 212090 309680 3983900 5186500 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.38 (0.40) YES No 0.09(0.10) YES No 2.97 (2.90) YES No 0.40(0.40) YES No 43.08 (13.20) No No 18.91 (13.71) No Page 98 of 169 143 Services' Quantitative Peak Review | t^ PFBA 1 213.0/169.0 Da Baseline Missed n Wrnnn Pnak i 13C-PFBA 217.0/172.0 Da c Baseline c Missed ___ n Wrnnn Peak___ PFBS 1 299.0/80.0 Da Baseline c Missed 180-PFHS 403.0/103.0 Da Baseline Missed I 6.15 5.24 ... ! ?. o.'. l1l^. L ................ Ih & `.5 -a PFHpA 1 363.0/169.0 Da Baseline u Missed Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak p. 23 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM K1001011-013 I .L PFPeA 1 263.0/219.0 Da Baseline a Missed n Wrnnn Ppak I 13C-PFHxA 315.0/270.0 Da g Baseline Missed n Wrnnn Peak___ PFHxA 1 313.0/269.0 Da Baseline Missed Wrnnn Pnak 6^ 130-PFHxA 315.0/270.0 Da u Baseline Missed r Wrnnn Pnak PFHxS 1 399.0/80.0 Da Baseline u Missi n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak___ S_180-PFHS 403.0/103.0 Da a Baseline Missed ___r Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da Baseline c Missed ____n Wrnnn Pnak___ SJ3C-PFDA 515.0/470.0 Da Baseline a Missed ___ n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Pnak___ Page 99 o f 169 144 JFi C o lu m b ia Analytical Services" Ion Ratio Confirmation 3.54 p. 24 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM K1001011-013 PFBA2 213.0/169.0 Da Baseline a Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline a Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Thiswageisndavaiable PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da o Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da o Baseline u Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 100 of 169 145 p. 25 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-014 2/17/2010 9:46:52 PM PFCkinetexLong.dam 38 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFD0A PFTeA 1 13C-PFD0A IS Area 4282000 2928900 8452100 3300300 119280 8452100 160790 160790 160790 Analyte RT (Exp RT ) 1.20 (1.36) 2.01 (2.06) 1.99 (2.09) 0.00(3.06) 3.82 (3.88) 3.83 (3.87) 0.00 (4.55) 5.23 (5.06) 5.47 (5.51) Analyte Area 149310 25493 1021300 0 7104 4081 0 7430 7441 Calc. Cone (ng/mL) 0.716 0.114 1.150 N/A 0.025 0.025 N/A N/A 0.079 Modified? No No No No No No No No No 4 l<v 2/ '^ 7 0 * 1 9 /' Page 13 of 170 146 Services' p. 26 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 SJ3C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp R T ) 1.18(1.36) 1.18(1.36) 1.94 (2.08) 0.00 (2.44) 2.00 (2.12) 1.99 (2.00) 2.99 (3.06) 3.01 (3.04) 0.00(3.88) 3.80 (3.88) 0.00 (3.87) 0.00(4.53) 4.49 (4.64) 5.24 (5.13) 5.45 (5.56) Area 250700 4282000 3806 0 328370 8452100 680 3300300 0 119280 0 0 160790 220 694 Modified? No Calc (Exp) MRM Ratio 1.68 (2.16) Ratio Confirms ID No No 28.68 (43.19) No No 0.15(0.34) No No 0.00(0.00) No No 0.32(0.32) YES No 8.28 (21.71) No No 0.00(0.14) No No 0.00(17.53) No No 0.00 (0.19) No No 16.79(1.60) No No 0.00 (0.32) No No 0.00(0.28) No No 0.00 (3.71) No No 0.03 (0.34) No No 0.09(0.29) No Page 14 of 170 147 p. 27 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Quantitative Peak Review JL Li1.20 ! 1,18 PFOA 1 413.0/169.0 Da Baseline Missed ri Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wrnnn Peak L K1001011-014 JL u L2.01 PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak !ill, I>' li i,V' !/< 13C-PFNA 468.0/423.0 Da Baseline c Missed n Wrnnn Peak ________ 3^4 PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak 3.82 PFUnA 1 563.0/519.0 Da Baseline Missed ri Wrnnn Peak___ PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Pnak 5.47 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Pnak 3.80 i 13C-PFUnA 565.0/270.0 Da Baseline Missed ------n Wrnnn Peak___ I4.49 So 13C-PFDOA 1615.0/169.0 Da Baseline o Missed n Wrnnn Paak 4.49 PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Pnak '006 DUO i '>W 1 3.83 -/ A ,M k . , ! W v ^ , 13C-PFDA 515.0/470.0 Da Baseline Missed r Wrnnn Pnak 6S PFDS 1 599.0/80.0 Da Baseline Missed p Wrnnn PsaK 13C-PFOS 503.0/80.0 Da Baseline c Missed Wrnnn Fnak.. tSM WS io - 2-* soon 5.23 14 JS `jit '.* 60 PFTrA 1 663.0/619.0 Da Baseline c Missed n Wrnnn Paak 13C-PFDoA 615.0/169.0 Da c Baseline Misse n Wrnnn Peak PFTeA 1 713.0/669.0 Da Baseline Missed ___n Wrnnn Peak 13C-PFDoA 615.0/169.0 Da c Baseline Missed ___r Wrnnn Paak___ Before After Page 15 of 170 148 ium bia Ion Ratio Confirmation p. 28 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM K1001011-014 Before After Page 16 o f 170 149 S e rv ic e s " p. 29 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-014 2/18/2010 4:13:31 AM PFCkinetexShort.dam 38 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 5642700 7074200 4192900 7074200 7074200 4192900 4585900 4585900 Analyte RT (Exp RT ) 1.25 (1.27) 3.53 (3.60) 4.19 (4.26) 5.23 (5.31) 6.14 (6.23) 6.23 (6.32) 6.23 (6.31) 7.51 (7.65) Analyte Area 132790 291980 170880 340730 77424 986730 4192900 4585900 Calc. Cone (ng/mL) 0.16 0.43 0.13 0.42 0.25 0.70 1.13 1.00 Modified? No No No No No No No No Page 101 of 169 150 p. 30 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.25(1.27) 3.53(3.60) 4.19(4.26) 5.24(5.31) 6.14(6.22) 6.23 (6.32) 1.23(1.26) 5.23(5.30) Area 132790 291980 65485 29792 229180 393260 5642700 7074200 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.38(0.40) YES No 0.09(0.10) YES No 2.96 (2.90) YES No 0.40(0.40) YES No 42.49(13.20) No No 20.76(13.71) No Page 102 of 169 151 iumbia sivticai Quantitative Peak Review PFBA 1 213.0/169.0 Da g Baseline u Missed n Wronn Peak 4^9 13C-PFBA 217.0/172.0 Da Baseline Missed ___n Wronn Peak___ PFBS 1 299.0/80.0 Da Baseline Missed n Wrnnn Peak 6^ 180-PFHS 403.0/103.0 Da q Baseline Missed ___r Wrnnn Peak__ _ p. 31 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM 1 ..K1001011-014 PFPeA 1 263.0/219.0 Da Baseline Missed n Wronn Pnak 13C-PFHxA 315.0/270.0 Da d Baseline g Missed n Wronn Peak___ i5.23 PFHxA 1 313.0/269.0 Da Baseline c Missed r. Wrnnn Pnak 130-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak 00*5 so*5 PFHpA 1 363.0/169.0 Da Baseline c Missed ___n Wrnnn Peak S_180-PFHS 403.0/103.0 Da c Baseline Missed n Wrnnn Peak 130-PFHxA 315.0/270.0 Da Baseline g Missed ___p Wrnnn Peak___ 7.51 PFHxS 1 399.0/80.0 Da Baseline g Missed ___n Wmnn Peak___ 13*5 7.51 HIV1- sites 40$ 30*5____ L 0*5 *i GlteS __ _ 30*5 __ L 13C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak___ SJ3C-PFDA 515.0/470.0 Da c Baseline Missed ____n Wrnnn Peak Before After 180-PFHS 403.0/103.0 Da Baseline Missed Wrnnn Peak 7. 1 -UteS t 60*5 ` 4>1*5 J0*5 13C-PFDA 515.0/470.0 Da c Baseline a Missed n Wrnnn Peak___ Page 103 of 169 152 p. 32 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation 1.25 J l_ K1001011-014 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed u Wrong Peak PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak 6.14 ...... ... 6.B3.............- ' - ......... i 10^ f............... . 5oh ......." A| ............. 5`, 70 6C -6 *0 PFHpA2 363.0/319.0 Da c Baseline c Missed c Wrong Peak PFHxS 2 399.0/99.0 Da o Baseline u Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Ihisinageisnotavaiable N/A Baseline o Missed Wrong Peak Page 104 o f 169 153 Iiimba Services" p. 33 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\Samplesl_ong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-015 2/17/2010 9:54:29 PM PFCkinetexLong.dam 39 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 130-PFDoA PFTrA 1 13C-PFDOA PFTeA 1 130-PFDoA IS Area 4027200 2707700 8282800 3145300 116130 8282800 168000 168000 168000 Analyte RT (Exp RT ) 1.18 (1.36) 2.00 (2.06) 1.98 (2.09) 2.98 (3.06) 3.85 (4.06) 3.85 (3.87) 4.47 (4.55) 5.04 (5.06) 5.50 (5.51) Analyte Area 69653 16308 741590 28743 21568 37821 14563 11652 5809 Calc. Cone (ng/mL) 0.370 0.090 0.852 0.080 0.146 0.059 0.151 0.001 0.031 Modified? No No No No No No No No No J Page 106 of 170 154 p. 34 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM IRC Summary ; Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp R T ) 1.22(1.36) 1.22(1.36) 1.93 (2.08) 0.00 (2.44) 1.97(2.12) 1.99 (2.00) 3.02(3.06) 3.02 (3.04) 3.83(3.88) 3.83 (3.88) 3.83(3.87) 4.48 (4.53) 4.49 (4.64) 5.04(5.13) 5.50(5.56) Area 102280 4027200 3012 0 254070 8282800 3138 3145300 4500 116130 12584 3725 168000 2159 1298 Modified? Calc (Exp) MRM Ratio No 1.47 (2.16) Ratio Confirms ID No No 57.82 (43.19) No No 0.18(0.34) No No 0.00 (0.00) No No 0.34(0.32) YES No 11.17 (21.71) No No 0.11 (0.14) YES No 109.43(17.53) No No 0.21 (0.19) YES No 5.38(1.60) No No 0.33(0.32) YES No 0.26(0.28) YES No 11.54(3.71) No No 0.19(0.34) No No 0.22 (0.29) YES Page 107 o f 170 155 p. 35 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review I PFOA 1 413.0/169.0 Da Baseline i: Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline u Missed ------n Wrnnn Peak----"H K1001011-015 A1.96 2 00 PFNA 1 463.0/419.0 Da Baseline c Missed in Wrnnn Paak 13C-PFNA 1468.0/423.0 Da Baseline c Missed r Wrnnn Peak___ 3.02 PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak PFUnA 1 563.0/519.0 Da c Baseline Missed n Wrnnn Paak 4.47 .-.-kuPFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da o Baseline Missed n Wrnnn Paak JL_ PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 3^5 13C-PFUnA 565.0/270.0 Da Baseline u Missed n Wrnnn Peak___ 4.49 L _________ _________ 130-PFDoA 615.0/169.0 Da Baseline c Missed n Wrnnn Peak PFDS 1 599.0/80.0 Da Baseline Missed ____n Wrnnn Peak___ .'n 1I1 ? MW) 40X1 :-coo 5.04 PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed r Wrnnn Peak L 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak___ 4^3 34 130-PFDoA 615.0/169.0 Da o Baseline Missed ____n Wrnnn Peak___ 5.50 PFTeA 1 713.0/669.0 Da Baseline Missed ___n Wrnnn Peak___ 13C-PFD0A 615.0/169.0 Da o Baseline Missed p Wrnnn Peak Before After Page 108 of 170 156 p. 36 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Ion Ratio Confirmation 1.22 `-m i yjto .L- A . PFOA2 413.0/369.0 Da Baseline c Missed l Wrong Peak >s 20 20 30 PFNA2 463.0/219.0 Da a Baseline Missed Wrong Peak K1001011-015 5H k AA $ ** / Va\..... .... ... : ;`M N. ! /A l`- :t! 25 30 PFOS2 499.0/99.0 Da o Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da a Baseline c Missed Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak 1 ............. i ; . r > Tur.^ ifan PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak IJUMIJ r iciouu i.# ' Tms-nii- PFDoA2 613.0/169.0 Da c Baseline Missed Wrong Peak l.coo l&tXnl * SCwO J i.Cidi " JOflO 2000 PFTrA 2 663.0/169.0 Da o Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 109 o f 170 157 p. 37 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's ProjectstPFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-015 2/18/2010 4:23:06 AM PFCkinetexShort.dam 39 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5385400 6508100 4196500 6508100 6508100 4196500 4950500 4950500 Analyte RT (Exp RT ) 1.24 (1.27) 3.53 (3.60) 4.19 (4.26) 5.23 (5.31) 6.12 (6.23) 6.22 (6.32) 6.22 (6.31) 7.52 (7.65) Analyte Area 78521 127470 174230 200550 45767 1265800 4196500 4950500 Calc. Cone (ng/mL) 0.08 0.20 0.14 0.25 0.14 0.90 1.05 1.00 Modified? No No No No No No No No zJ < Page 105 of 169 158 p. 38 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary : Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.24(1.27) 3.53(3.60) 4.19(4.26) 5.24 (5.31) 6.13(6.22) 6.22 (6.32) 1.24 (1.26) 5.23 (5.30) Area 78521 127470 62153 18391 131540 501760 5385400 6508100 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.36 (0.40) YES No 0.09(0.10) YES No 2.87(2.90) YES No 0.40(0.40) YES No 68.59 (13.20) No No 32.45(13.71) No Page 106 o f 169 159 p. 39 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM 1 L LQuantitative Peak Review K1001011-015 I PFBA 1 213.0/169.0 Da Baseline l. Missed r Wrnnn Peak 4^9 PFBS 1 299.0/80.0 Da Baseline c Missed r Wrnnn Peak 6.12 13C-PFBA 217.0/172.0 Da Baseline c Missed n Wrnnn Peak___ 6.22 1 1 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak PFPeA 1 263.0/219.0 Da Baseline a Missed n Wrnnn Peak 5.23 1*4 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak___ ? 1Hrf 1 1 2 >,6*5 I ... . J L `S_______ _6D PFHxA 1 313.0/269.0 Da Baseline c Missed n Wrnnn Ppak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Pftak ____ JL . PFHpA 1 363.0/169.0 Da Baseline c Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da Baseline Missed n Wronn Peak___ 180-PFHS 403.0/103.0 Da Baseline c Missed r Wrnnn Peak S_180-PFHS 403.0/103.0 Da Baseline Missed ___ Wrnnn .Peak___ 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ___n Wrnnn Peak___ Page 107 of 169 160 SrCSK Ion Ratio Confirmation p. 40 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM K1001011-015 PFBA2 213.0/169.0 Da u: Baseline o Missed l Wrong Peak 6.13 PFPeA2 263.0/219.0 Da d Baseline o Missed a Wrong Peak PFBS2 299.0/99.0 Da Baseline u Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline c Missed c Wrong Peak PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak SJ3C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline u Missed Wrong Peak Page 108 of 169 161 Services' p. 41 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-016 2/17/2010 10:02:01 PM PFCkinetexLong.dam 40 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3848900 2368800 7153900 2786900 101620 7153900 148660 148660 148660 Analyte RT (Exp RT ) 1.23 (1.36) 2.01 (2.06) 2.04 (2.09) 0.00 (3.06) 0.00(4.06) 3.99 (3.87) 0.00 (4.55) 5.04 (5.06) 0.00(5.51) Analyte Area 68449 6977 615350 0 0 1051 0 7028 0 Calc. Cone (ng/mL) 0.379 0.061 0.820 N/A N/A 0.022 N/A N/A N/A Modified? No No No No No No No No No Z ~ (( Page 110 o f 170 162 p. 42 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM IRC Summary ] Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.22(1.36) 1.21 (1.36) 2.02 (2.08) 0.00 (2.44) 2.04 (2.12) 2.04 (2.00) 3.01 (3.06) 3.04 (3.04) 0.00(3.88) 3.84(3.88) 0.00 (3.87) 0.00 (4.53) 4.49 (4.64) 5.29 (5.13) 5.48(5.56) Area 113190 3848900 2665 0 197280 7153900 574 2786900 0 101620 0 0 148660 257 209 Modified? Calc (Exp) MRM Ratio No 1.65 (2.16) Ratio Confirms ID No No 56.23 (43.19) No No 0.38(0.34) YES No 0.00 (0.00) No No 0.32(0.32) YES No 11.63 (21.71) No No 0.00 (0.14) No No 0.00(17.53) No No 0.00 (0.19) No No 0.00(1.60) No No 0.00(0.32) No No 0.00 (0.28) No No 0.00(3.71) No No 0.04 (0.34) No No 0.00 (0.29) No Page 111 o f 170 163 p. 43 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review K1001011-016 1.21 ..ul t ? 2.oi l t uxw ' K1.it m( ''! _______1 _______ j PFOA 1 413.0/169.0 Da u Baseline Missed n Wronn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wronn Peak PFNA 1 463.0/419.0 Da Baseline n Missed n Wronn Paak 13C-PFNA 468.0/423.0 Da Baseline Missed Wronn Peak PFOS 1 499.0/80.0 Da d Baseline c Missed r: Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n Wronn Peak PFUnA 1 563.0/519.0 Da a Baseline Missed ___ n Wronn Peak 13C-PFUnA 565.0/270.0 Da Baseline c Missed __ p Wronn.PfifiK__ 4^9 PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Pnak 3.99 PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline c Missed n Wrnnn Paak ; 2.04 i 13C-PFOS 503.0/80.0 Da Baseline Missed ____n Wrnnn Peak___ 4^9 PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Paak 13C-PFDOA 615.0/169.0 Da d Baseline Missed n Wrnnn Paak 5.04 PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Paak___ PFTeA 1 713.0/669.0 Da Baseline Missed ___r Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline a Missed n Wrnnn Peak___ Before After 164 Page 112 o f 170 Services' p. 44 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Ion Ratio Confirmation K1001011-016 1.22 I F 1 ma- JV A5 - . 1Cj f? SQ'lO-i :o :$ 30 ", ", ;s........in U'_ :s -r is if' PFOA2 413.0/369.0 Da Baseline d Missed g Wrong Peak PFNA 2 463.0/219.0 Da a Baseline u Missed u Wrong Peak PFOS2 499.0/99.0 Da Baseline u Missed Wrong Peak PFDA2 513.0/269.0 Da d Baseline Missed g Wrong Peak twoj } - PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak ~~ |Otic 30 !S <0 JS PFDS2 599.0/99.0 Da Baseline Missed a Wrong Peak mt: 2 sco PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak 8 lOCrOtj soon ~ --. . -----. . . .'~v PFTrA 2 663.0/169.0 Da Baseline c Misse c Wrong Peak PFTeA2 713.0/169.0 Da g Baseline Missed g Wrong Peak Before After Page 113 o f 170 165 Columbia Analytical Sorm os' Created with Analyit'Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-016 2/18/2010 4:32:41 AM PFCkinetexShort.dam 40 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 824050 5695200 3422900 5695200 5695200 3422900 4323500 4323500 Analyte RT (Exp RT ) 1.24 (1.27) 3.53 (3.60) 4.19 (4.26) 5.23 (5.31) 6.14 (6.23) 6.23 (6.32) 6.23 (6.31) 7.52 (7.65) Analyte Area 20324 91549 135500 185620 45197 1148600 3422900 4323500 Calc. Cone (ng/mL) 0.17 0.16 0.13 0.26 0.17 1.00 0.98 1.00 Modified? No No No No No No No No q \\0 2^ / / Page 109 of 169 166 ium bia p. 46 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary ' Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.24(1.27) 3.53 (3.60) 4.19(4.26) 5.23(5.31) 6.15(6.22) 6.22 (6.32) 1.24(1.26) 5.23(5.30) Area 20324 91549 50754 15491 134270 432750 824050 5695200 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.37 (0.40) YES No 0.08(0.10) YES No 2.97(2.90) YES No 0.38(0.40) YES No 40.55(13.20) No No 30.68(13.71) No Page 110 o f 169 167 p. 47 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Quantitative Peak Review i A1.24 K1001011-016 PFBA 1 213.0/169.0 Da Baseline g Missed n Wrnnn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed __ r Wrnnn Pfiah__ PFPeA 1 263.0/219.0 Da Baseline c Missed r Wrnnn Poak L PFBS 1 299.0/80.0 Da Baseline c Missed r Wrnnn Peak 6.14 PFHpA 1 363.0/169.0 Da g Baseline g Missed n Wrnnn Peak ra] 6,2r3 ::i I I " I ft i ____ J L S J 8 0 -P F H S 403.0/103.0 Da Baseline Missed ___ Wrnnn Peak___ 180-PFHS 403.0/103.0 Da Baseline Missed 11 Wrnnn Ppak PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Peak ! 6.23 ;>fwui! rI **] II 50*J B 1)0*0L............ .... 13C-PFHXA 315.0/270.0 Da Baseline u Missed m HPeS i SBt?. 4lie?, n Wronn Peak 7.512 1 1 I ML PFHxS 1 399.0/80.0 Da Baseline Missed p Wrnnn PfiaK U L:o*s OM 13C-PFDA 515.0/470.0 Da Baseline Missed ___n W rnnn Peak___ SJ3C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak Before After 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFHXA 315.0/270.0 Da Baseline c Missed r Wrnnn Peak [6.23 i i f. 0 H 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak___ 7.52 Li 13C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrnnn Ppak___ -5 i Page 111 o f 169 168 p. 48 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation K1001011-016 1.24 i At J *> PFBA2 213.0/169.0 Da c Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da c Baseline c Missed Wrong Peak L. PFBS2 299.0/99.0 Da Baseline d Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline u Missed Wrong Peak 6.1-5 i e.fe; >0*4 j . PFHpA2 363.0/319.0 Da Baseline Missed c Wrong Peak PFHxS2 399.0/99.0 Da Baseline c Missed o Wrong Peak SJ3C-PFDA 515.0/470.0 Da Baseline c Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After Tirinageisnotavaiable N/A Baseline Missed Wrong Peak Page 112 of 169 169 p. 49 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 021710\SamplesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-017 2/17/2010 10:09:37 PM PFCkinetexLong.dam 41 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFD0A PFTeA 1 13C-PFDOA IS Area 3967000 2756400 8420200 3174100 108950 8420200 177880 177880 177880 Analyte RT (Exp RT ) 1.22 (1.36) 1.96 (2.06) 2.00 (2.09) 0.00(3.06) 0.00 (3.88) 4.04 (3.87) 0.00 (4.55) 5.23 (5.06) 5.71 (5.51) Analyte Area Calc. Cone (ng/mL) 65500 0.354 -#* */ 11086-------- --------t o t J 492520 0.562 0 N/A 0 N/A f in ji -3i f ---------- ----- U b 0 N/A 6232 N/A fttm 7I7T -5888--------- ------------ ^/X7 Modified? No No No No No No No No No no z, / ` * Page 25 o f 170 170 Services' p. 50 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.22(1.36) 1.23(1.36) 1.92 (2.08) 0.00 (2.44) 1.99 (2.12) 1.98(2.00) 3.06 (3.06) 3.03 (3.04) 0.00(3.88) 3.82 (3.88) 0.00 (3.87) 0.00 (4.53) 4.46 (4.64) 5.16 (5.13) 5.52 (5.56) Area 97062 3967000 2985 0 155780 8420200 294 3174100 0 108950 0 0 177880 388 240 Modified? Calc (Exp) MRM Ratio No 1.48 (2.16) Ratio Confirms ID No No 60.56 (43.19) No No 0.25 (0.34) No No 0.00 (0.00) No No 0.32(0.32) YES No 17.10(21.71) No No 0.00 (0.14) No No 0.00(17.53) No No 0.00(0.19) No No 0.00(1.60) No No 0.00 (0.32) No No 0.00 (0.28) No No 0.00(3.71) No No 0.06(0.34) No No 0.04 (0.29) No Page 26 of 170 171 / T C olilR lbilS l L a Analytical Services" Quantitative Peak Review .22 p. 51 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM K1001011-017 PFOA 1 413.0/169.0 Da g Baseline c Missed ri Wrnnn Paak 13C-PFOA 417.0/372.0 Da o Baseline Missed n Wrnnn Peak ______ ___________ PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Paak 13C-PFNA 468.0/423.0 Da Baseline Missed ___n Wrnnn Peak___ PFOS 1 499.0/80.0 Da Baseline Missed r. Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak ______ 3 ^ _________ PFDA 1 513.0/469.0 Da Baseline Missed ___n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed Wrnnn Peak PFUnA 1 563.0/519.0 Da Baseline Missed n Wrnnn Peak ""I PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 5.71 PFTeA 1 713.0/669.0 Da Baseline Missed ___ n Wrnnn Paak 13C-PFUnA 565.0/270.0 Da Baseline c Missed n Wrnnn Peak 4.46 I 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak I 4.46 Li PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak mr. won 5.23 13C-PFOS 503.0/80.0 Da Baseline c Missed l*4 Wrnnn Peak 4.46 *4 1 PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Paak______ 130-PFDoA 615.0/169.0 Da Baseline Missed n Wrnnn Peak___ 130-PFDoA 615.0/169.0 Da n Baseline Missed ___ n Wrnnn Peak Before After Page 27 o f 170 172 p. 52 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Ion Ratio Confirmation K1001011-017 lf Sc4 j' 1.22 1eJ 1.92 is :-r Mlft , s, *1 ' mow `two io i' :*, to 5 `-'i 35 <0 PFOA2 413.0/369.0 Da Baseline Missed g Wrong Peak PFNA 2 463.0/219.0 Da Baseline Missed Wrong Peak jut'. PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak u.'iit. ^ tO*0Ol! | 6000 4U jO PFDA2 513.0/269.0 Da Baseline Missed d Wrong Peak 1SC*0 Ififiiw 50)D PFUnA 2 563.0/269.0 Da o Baseline Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed g Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed g Wrong Peak PFTrA 2 663.0/169.0 Da Baseline o Missed g Wrong Peak Mi 5`. 60 PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 28 of 170 173 f C o lu m b ia w i Analytical S m m w T p. 53 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 021710\SamplesShort. wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-017 2/18/2010 4:42:13 AM PFCkinetexShort.dam 41 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHXA PFHxS1 180-PFHS S_180-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 5334200 6702200 3931100 6702200 6702200 3931100 4924800 4924800 Analyte RT (Exp RT ) 1.23 (1.27) 3.53 (3.60) 4.18 (4.26) 5.23 (5.31) 6.14 (6.23) 6.24 (6.32) 6.23 (6.31) 7.54 (7.65) Analyte Area 81761 144930 101780 163070 34858 385530 3931100 4924800 Calc. Cone (ng/mL) 0.08 0.22 0.09 0.18 0.09 0.28 0.99 1.00 Modified? No No No No No No No No s Page 113 o f 169 174 p. 54 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.23(1.27) 3.53 (3.60) 4.18 (4.26) 5.22 (5.31) 6.15(6.22) 6.23 (6.32) 1.23(1.26) 5.22(5.30) Area 81761 144930 39095 14405 114080 155860 5334200 6702200 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.38(0.40) YES No 0.09(0.10) YES No 3.27(2.90) YES No 0.40(0.40) YES No 65.24 (13.20) No No 41.10(13.71) No Page 114 o f 169 175 d o ltin rs fo is Armlvfiiol Services" p. 55 Created with Analyst Reporter Printed! 18/02/2010 4!02!00 PM Quantitative Peak Review 1.23 PFBA 1 1213.0/169.0 Da Baseline Missed n Wronn Peak 4.18 I__L _ 13C-PFBA 217.0/172.0 Da Baseline c Missed ___ n Wronn Peak___ K1001011-017 I PFPeA 1 1263.0/219.0 Da Baseline l Missed - Wrnnn Ppak 5.23 13C-PFHxA 315.0/270.0 Da c Baseline c Missed ___ n Wronn Peak----- PFBS 1 299.0/80.0 Da Baseline c Missed r Wrnnn Peak 6.14 18 0 -P F H S 403.0/103.0 Da Baseline c Missed r Wronn Peak PFHxA 1 313.0/269.0 Da Baseline u Missed n Wrnnn Peak___ 13C-PFHxA 315.0/270.0 Da Baseline n Missed n Wrnnn Peak M. PFHpA 1 363.0/169.0 Da Baseline Missed ___n Wronn Peak___ 13C-PFHXA 315.0/270.0 Da u Baseline c Missed ____n Wronn Peak___ PFHxS 1 399.0/80.0 Da Baseline c Missed ___n Wronn Peak___ 18 0 -P F H S 403.0/103.0 Da Baseline Missed n Wrnnn Peak S_180-PFHS 403.0/103.0 Da Baseline Missed ___ n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak___ S_13C-PFDA 515.0/470.0 Da c Baseline Missed n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___ Page 115 of 169 176 p. 56 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation " b .. ........................... : ..... 3.53 - ; i i f \ 1 ............ <c> := :a PFBA 2 213.0/169.0 Da Baseline g Missed Wrong Peak i : : ! ....... I ......... c*t Ml M JO <5 PFPeA 2 263.0/219.0 Da o Baseline Missed c Wrong Peak K1001011-017 =* %nion J 1(11)80 SO 1 . 4.ffj8.. . ... )5 `C *5 50 PFBS 2 299.0/99.0 Da Baseline c Missed Wrong Peak 1 f. I 45 u PFHxA 2 313.0/119.0 Da Baseline c Missed g Wrong Peak 6.15 L .......... - 6. 3--............- PFHpA2 363.0/319.0 Da u Baseline g Missed c Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed g Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 116 of 169 177 $ rvkm ' Created with Anafy^?'R*eporter Printed: 19/02/2010 11:59:00 AM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02171 OVSamplesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-018 2/17/2010 10:17:12 PM PFCkinetexLong.dam 42 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 130-PFDoA IS Area 3997500 2750600 8544600 2954300 155620 8544600 303910 303910 303910 Analyte RT (Exp RT ) 1.23 (1.36) 2.06 (2.06) 2.05 (2.09) 0.00 (3.06) 3.85 (3.88) 3.86 (3.87) 4.50 (4.55) 5.03 (5.06) 5.50 (5.51) Analyte Area 58048 12506 312660 0 18551 61025 14090 7648 5367 Calc. Cone (ng/mL) 0.315 0.076 0.357 N/A 0.082 0.081 0.084 N/A N/A Modified? No No No No No No No No No ri U * ItO Z. ! 1 T Page 29 o f 170 178 p. 58 Created with Analyst Reporter J! Printed: 19/02/2010 11:59:00 AM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.25(1.36) 1.23(1.36) 2.04 (2.08) 0.00 (2.44) 2.06 (2.12) 2.03 (2.00) 3.06 (3.06) 3.03 (3.04) 3.88 (3.88) 3.85 (3.88) 3.87 (3.87) 4.52 (4.53) 4.52 (4.64) 5.03(5.13) 5.51 (5.56) Area 82975 3997500 2855 0 98726 8544600 788 2954300 2663 155620 21914 3704 303910 2136 1067 Modified? Calc (Exp) MRM Ratio No 1.43 (2.16) Ratio Confirms ID No No 68.87 (43.19) No No 0.23(0.34) No No 0.00(0.00) No No 0.32(0.32) YES No 27.33 (21.71) No No 0.00 (0.14) No No 0.00(17.53) No No 0.14(0.19) YES No 8.39(1.60) No No 0.36(0.32) YES No 0.26(0.28) YES No 21.57(3.71) No No 0.28(0.34) YES No 0.20 (0.29) No Page 30 of 170 179 1Quantitative Peak Review 1.23 PFOA 1 413.0/169.0 Da Baseline i_ Missed n Wrnnn Peak 2.05 13C-PFOA 417.0/372.0 Da Baseline e Missed n Wronn Peak 2^^ p. 59 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM K1001011-018 PFNA 1 463.0/419.0 Da Baseline c Missed r Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline c Missed Wrnnn Pf?ak PFOS 1 499.0/80.0 Da Baseline Missed r. Wrnnn Peak 3.85 PFUnA 1 563.0/519.0 Da q Baseline Missed ___ n Wrnnn Peak 4.50 A PFDoA 1 613.0/569.0 Da Baseline o Missed n Wrnnn Peak 13C-PFOS 1503.0/80.0 Da Baseline a Missed n Wrnnn Peak 3.85 PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 13C-PFUnA 565.0/270.0 Da Baseline Missed Wrnnn Peak j______ 4 ^ _________ PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak___ Mm1 I'.onnJ 5.03 13C-PFDOA 615.0/169.0 Da Baseline a Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da c Baseline c Missed n Wrnnn Peak ju 13C-PFOS 503.0/80.0 Da Baseline Missed ____n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da q Baseline Missed n Wrnnn Peak 5.50 PFTeA 1 713.0/669.0 Da Baseline Missed ___- .W rnnn Peak___ 13C-PFDoA 615.0/169.0 Da Baseline Missed ___n Wrnnn Peak___ Before After Page 31 o f 170 180 Services" p. 60 Created with Analyst Reporter Printed: 19/02/2010 11:59:00 AM Ion Ratio Confirmation K1001011-018 1.25 PFOA2 413.0/369.0 Da Baseline Missed a Wrong Peak PFUnA2 563.0/269.0 Da Baseline o Missed l Wrong Peak 2.04 1 leJ A 6- lVMi ? lljilflll *>CKiU . ... 3.06.. - PFNA2 463.0/219.0 Da Baseline Missed c Wrong Peak ,,i PFDS 2 599.0/99.0 Da Baseline Missed Wrong Peak PFOS2 499.0/99.0 Da Baseline Missed c Wrong Peak ; `-an a* 'UOOO i scran ______4y$2_______ *b sa PFDoA2 613.0/169.0 Da D Baseline c Missed a Wrong Peak PFDA2 513.0/269.0 Da Baseline c Missed a Wrong Peak csom, ;rarm ] | IdCfiii """l 5.03 PFTA2 663.0/169.0 Da Baseline Missed c Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 32 o f 170 181 Services- p. 61 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-018 2/18/2010 4:51:45 AM PFCkinetexShort.dam 42 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 5804400 6964500 3836700 6964500 6964500 3836700 4471900 4471900 Analyte RT (Exp RT ) 1.23 (1.27) 3.53 (3.60) 4.19 (4.26) 5.24 (5.31) 6.16 (6.23) 6.25 (6.32) 6.25 (6.31) 7.55 (7.65) Analyte Area 101120 117070 89654 162880 36202 266200 3836700 4471900 Calc. Cone (ng/mL) 0.10 0.17 0.09 0.17 0.09 0.20 1.06 1.00 Modified? No No No No No No No No 2(10 (( V Page 117 of 169 182 Services' p. 62 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.23 (1.27) 3.53(3.60) 4.20 (4.26) 5.24(5.31) 6.16(6.22) 6.25 (6.32) 1.23(1.26) 5.24(5.30) Area 101120 117070 33834 15886 111520 102390 5804400 6964500 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.38(0.40) YES No 0.10(0.10) YES No 3.08 (2.90) YES No 0.38(0.40) YES No 57.40(13.20) No No 42.76(13.71) No Page 118 of 169 183 Services- Quantitative Peak Review 1.23 PFBA 1 213.0/169.0 Da c Baseline c Missed r ;Wronn Peak n :o 13C-PFBA 217.0/172.0 Da Baseline u Missed ___r~Wronn Peak___ p. 63 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM K1001011-018 PFPeA 1 263.0/219.0 Da Baseline u Missed n Wronn Peak 5.24 13C-PFHxA 315.0/270.0 Da Baseline c Missed ___r Wronn Peak___ PBS 1 299.0/80.0 Da a Baseline o Missed n Wrnnn Peak 6.16 180-PFHS 403.0/103.0 Da o Baseline c Missed r- Wrnnn Ppak PFHxA 1 313.0/269.0 Da c Baseline Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline c Missed r Wrnnn Ppak PFHpA 1 363.0/169.0 Da c Baseline Missed ___n Wronn Peak___ 13C-PFHXA 315.0/270.0 Da Baseline Missed ___n Wrnnn Peak ! a0*51 .-,VC, PFHxS 1 399.0/80.0 Da Baseline c Missed n Wrnnn Peak___ 180-PFHS 403.0/103.0 Da d Baseline Missed __ p Wronn PeaK__ S_180-PFHS 403.0/103.0 Da Baseline a Missed ___ n W rong Peak___ 13C-PFDA 515.0/470.0 Da Baseline Missed ___ n W rnnn Peak___ S_13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak___ Page 119 of 169 184 p. 64 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation K1001011-018 I 1.23 ................. i ....... ............. HJ'XI) 1 ...... `uOu , tSOHO ^ 1iiOuCi .....- ..... 4. 20 PFBA2 213.0/169.0 Da Baseline l Missed c Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed c Wrong Peak PFBS2 299.0/99.0 Da c Baseline o Missed c Wrong Peak PFHxA2 313.0/119.0 Da Baseline Missed Wrong Peak wJ i 6.16 X i '^*4 ...............6. 15 .............. f !'kA t,Ui l iitf'. :-fwS PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline a Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline o Missed Wrong Peak Page 120 of 169 185 / ? C o lu m b ia Analytical Services'' p. 65 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesLong .wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-019 2/17/2010 10:24:43 PM PFCkinetexLong.dam 43 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFD0A PFTeA 1 13C-PFD0A IS Area 4089900 2974900 9540700 2900400 133900 9540700 193780 193780 193780 Analyte RT (Exp RT ) 1.23 (1.36) 2.05 (2.06) 2.06 (2.09) 0.00(3.06) 3.90 (4.06) 3.89 (3.87) 0.00(4.55) 5.24 (5.06) 0.00(5.51) Analyte Area 975690 Calc. Cone (ng/mL) 4.730 9366 0.063 389500 0.397 0 N/A ^ - - 0 0 5 0 - f/9 6173 0.026 0 N/A 1742 N/A 0 N/A Modified? No No No No No No No No No n i 1 Page 122 of 170 186 ................................................ C o lt! HIPSIsis 4 *3 A m hikM Services'' p. 66 Created with Analyst Reporter Printed! 18/02/2010 3!24!00 PM IRC Summary Analyte Peak Name | !i PFO A2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDOA PFTrA 2 PFTeA2 RT (Exp R T ) 1.27(1.36) 1.21 (1.36) 2.02 (2.08) 0.00 (2.44) 2.04 (2.12) 2.06 (2.00) 3.03 (3.06) 3.04 (3.04) 0.00(3.88) 3.88(3.88) 0.00 (3.87) 0.00 (4.53) 4.54 (4.64) 5.04(5.13) 5.58(5.56) Area 1883600 4089900 1161 0 126530 9540700 765 2900400 0 133900 0 0 193780 390 164 Modified? Calc (Exp) MRM Ratio NO 1.93(2.16) Ratio Confirms ID YES No 4.19(43.19) No No 0.12(0.34) No No 0.00(0.00) No No 0.32(0.32) YES No 24.49(21.71) YES No 0.00(0.14) No No 0.00(17.53) No No 0.00(0.19) No No 11.64(1.60) No No 0.00 (0.32) No No 0.00(0.28) No No 0.00 (3.71) No No 0.22 (0.34) No No 0.00 (0.29) No Page 123 of 170 187 p. 67 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM IQuantitative Peak Review ; L1.21 PFOA 1 413.0/169.0 Da c Baseline Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed r Wrnnn Peak ' 2.06 PFOS 1 499.0/80.0 Da Baseline c Missed p Wrnnn Peak 3.90 iD ML!_________________ 13C-PFOS 503.0/80.0 Da o Baseline Missed ,___n Wrnnn Peak K1001011-019 2.05 ,r " PFNA 1 463.0/419.0 Da Baseline Missed i Wrnnn Peak JL 13C-PFNA 468.0/423.0 Da Baseline Missed Wronn Peak 3.04 L PFDA 1 513.0/469.0 Da Baseline u Missed p Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline c Missed r Wrnnn Peak PFUnA 1 563.0/519.0 Da c Baseline Missed ___p Wrnnn Peak !i*.W J PFDoA 1 613.0/569.0 Da d Baseline Missed n Wrnnn Peak 3.89 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak ______ 4 ^ _________ PFDS 1 599.0/80.0 Da Baseline Missed ___n Wrnnn Peak 0 J 5.24 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da c Baseline Missed ____n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline c Missed Wrnnn Peak 13C-PFDOA 615.0/169.0 Da d Baseline o Missed ____n Wrnnn Peak___ PFTeA 1 713.0/669.0 Da Baseline Missed ___n Wrnnn Peak ,L 13C-PFDOA 615.0/169.0 Da Baseline Missed ____n Wrnnn Peak Before After Page 124 o f 170 188 S ervie' Ion Ratio Confirmation Created with Analy^'F^eporter Printed: 18/02/2010 3:24:00 PM K1001011-019 Before After Page 125 of 170 189 p. 69 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-019 2/18/2010 5:01:18 AM PFCkinetexShort.dam 43 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5210000 6667800 4452800 6667800 6667800 4452800 4112800 4112800 Analyte RT (Exp RT ) 1.24 (1.27) 3.54 (3.60) 4.20 (4.26) 5.25 (5.31) 6.16 (6.23) 6.26 (6.32) 6.25 (6.31) 7.55 (7.65) Analyte Area 72943 141380 197990 239440 66041 592000 4452800 4112800 Calc. Cone (ng/mL) 0.07 0.21 0.14 0.30 0.22 0.39 1.34 1.00 Modified? No No No No No No No No i,/ Page 121 o f 169 190 Createci with Analyst' Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 ' PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.24(1.27) 3.54(3.60) 4.20 (4.26) 5.25 (5.31) 6.16(6.22) 6.25 (6.32) 1.23(1.26) 5.25 (5.30) Area 72943 141380 72821 22867 217250 218250 5210000 6667800 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.37(0.40) YES No 0.10(0.10) YES No 3.29(2.90) YES No 0.37 (0.40) YES No 71.43(13.20) No No 27.85(13.71) No Page 122 of 169 191 JTi Columbia C*.J A n a ly tic a l S s rv ic ^ r Quantitative Peak Review PFBA 1 213.0/169.0 Da Baseline Missed n Wrnnn Peak 13C-PFBA 217.0/172.0 Da Baseline c Missed ___p Wrnnn Peak___ p. 71 Created with Analyst Reporter Printed: 18/ 02/2010 4:02:00 PM K1001011-019 I PFPeA 1 263.0/219.0 Da c Baseline Missed r Wrnnn Pnak 5.^ 13C-PFHxA 315.0/270.0 Da Baseline Missed ___r. Wrnnn Peak___ PFBS1 299.0/80.0 Da Baseline Missed r Wrnnn Peak 6.16 180-PFHS 403.0/103.0 Da c Baseline Missed r Wrnnn Ppak PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Pnak 6.^ 13C-PFHXA 315.0/270.0 Da c Baseline Missed r: Wrnnn Ppak PFHpA 1 363.0/169.0 Da o Baseline o Missed ___ n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da d Baseline c Missed ____n Wrnnn Peak PFHxS 1 399.0/80.0 Da Baseline Missed ___n Wronn Peak___ i>0*i 180-PFHS 403.0/103.0 Da Baseline o Missed __ n Wrnnn PraK__ S_180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed ___n Wrnnn Pnak S_13C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak___ Page 123 o f 169 192 Colum bia Analytical S& m m s* Created with AnalyIV Importer Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation 1'I....j d ' ______ _______ n'. ir is ;n PFBA 2 213.0/169.0 Da Baseline Missed c Wrong Peak PFPeA2 263.0/219.0 Da Baseline c Missed Wrong Peak 6.16 .............6. 5 - .......... K1001011-019 s- .4 --- ------ t . - ... 4- ij.........-.jnJ-,k -- PFBS2 299.0/99.0 Da Baseline c Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline o Missed c Wrong Peak :n*4 0 N PFHpA2 363.0/319.0 Da Baseline Missed a Wrong Peak PFHxS2 399.0/99.0 Da o Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. N/A Baseline Missed a Wrong Peak Before After Page 124 of 169 193 p. 73 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 021710\Samplesl_ong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-020 2/17/2010 10:32:17 PM PFCkinetexLong.dam 44 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFDOA PFTeA 1 130-PFDoA IS Area 3984000 2666600 8043300 2939100 109160 8043300 146090 146090 146090 Analyte RT (Exp RT ) 1.23 (1.36) 2.05 (2.06) 2.09 (2.09) 0.00(3.06) 3.91 (4.06) 3.93 (3.87) 4.54 (4.55) 5.19 (5.06) 5.73 (5.51) Analyte Area 944430 17282 371500 0 10359 2601 5410 3792 7S73 Calc. Cone (ng/mL) 4.700 0.094 0.447 N/A 0.059 0.023 0.069 N/A 0 .104-- J9 Modified? No No No No No No No No No '5 '7 / / H o Page 126 of 170 194 Services' p. 74 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM IRC Summary ! Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.21 (1.36) 1.24(1.36) 2.07 (2.08) 0.00(2.44) 2.08 (2.12) 2.07 (2.00) 3.08(3.06) 3.09 (3.04) 0.00(3.88) 3.92(3.88) 0.00 (3.87) 0.00 (4.53) 4.55 (4.64) 5.08 (5.13) 5.55 (5.56) Area 1890100 3984000 5485 0 132060 8043300 638 2939100 0 109160 0 0 146090 407 215 Modified? Calc (Exp) MRM Ratio No 2.00(2.16) Ratio Confirms ID YES No 4.22 (43.19) No No 0.32(0.34) YES No 0.00 (0.00) No No 0.36(0.32) YES No 21.65 (21.71) YES No 0.00(0.14) No No 0.00(17.53) No No 0.00(0.19) No No 10.54(1.60) No No 0.00 (0.32) No No 0.00(0.28) No No 27.00(3.71) No No 0.11 (0.34) No No 0.03(0.29) No Page 127 o f 170 195 p. 75 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review i PFOA 1 413.0/169.0 Da c Baseline Missed p Wrnnn Peak 13C-PFOA 417.0/372.0 Da c Baseline Missed j i Wron2n.0P7eak _L PFOS 1 499.0/80.0 Da d Baseline Missed r Wrnnn Peak 3.91 13C-PFOS 503.0/80.0 Da Baseline d Missed n Wrnnn Peak PFUnA 1 563.0/519.0 Da o Baseline Missed ___p Wrnnn Peak 13C-PFUnA 565.0/270.0 Da Baseline o Missed ___ p Wrnnn Peak___ K1001011-020 i:nou tXita j 2.05 PFNA 1 463.0/419.0 Da c Baseline u Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da c Baseline c Missed Wrnnn Peak PFDA 1 513.0/469.0 Da Baseline Missed r Wrnnn Pnak 3.93100(1-j SuO-i PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFDA 1515.0/470.0 Da Baseline c Missed r Wrnnn Paak 13C-PFOS 503.0/80.0 Da c Baseline Missed ___n Wrnnn Peak___ 4.54 PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFD0A 615.0/169.0 Da Baseline Missed n Wrnnn Peak 5.19 PFTrA 1 663.0/619.0 Da Baseline Missed ___ n Wrnnn Pnak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed ___ n Wrnnn Pnak___ 5.73 PFTeA 1 713.0/669.0 Da Baseline Missed n Wrnnn Peak 5 S, 13C-PFDOA 615.0/169.0 Da d Baseline Missed ___n Wrnnn Peak Before After Page 128 o f 170 196 JT ClUTlfoiB Analytical Services" p. 76 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Ion Ratio Confirmation i1 ...... ' ...... :.. umnu i WJ00 PFOA 2 413.0/369.0 Da l Baseline o Missed g Wrong Peak i f :o ,,'s to PFNA2 463.0/219.0 Da Baseline Missed a Wrong Peak K1001011-020 AA j I ' 50 B PFOS2 499.0/99.0 Da Baseline Missed c Wrong Peak ICWI - UI1H < | i-onu IOOi PFDA 2 513.0/269.0 Da Baseline u Missed c Wrong Peak PFUnA2 563.0/269.0 Da c Baseline Missed u Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline g Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed g Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 129 of 170 197 p. 77 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1001011-020 2/18/2010 5:10:53 AM PFCkinetexShort.dam 44 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5161300 6494100 3963900 6494100 6494100 3963900 4811100 4811100 Analyte RT (Exp RT ) 1.22 (1.27) 3.53 (3.60) 4.20 (4.26) 5.25 (5.31) 6.16 (6.23) 6.25 (6.32) 6.25 (6.31) 7.55 (7.65) Analyte Area 89084 139640 201600 258180 70508 572060 3963900 4811100 Calc. Cone (ng/mL) 0.10 0.22 0.16 0.33 0.25 0.42 1.02 1.00 Modified? No No No No No No No No 4 tit> Page 125 o f 169 198 ................................................. ColllfTilbisS A n a ly tic a l S ervices" p. 78 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.22(1.27) 3.53 (3.60) 4.20 (4.26) 5.24(5.31) 6.16(6.22) 6.25 (6.32) 1.23 (1.26) 5.25(5.30) Area 89084 139640 74853 24000 233550 217740 5161300 6494100 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.37(0.40) YES No 0.09(0.10) YES No 3.31 (2.90) YES No 0.38 (0.40) YES No 57.94(13.20) No No 25.15(13.71) No Page 126 o f 169 199 .................................................. JT C o lu m b ia cL3 Analytical Services" Quantitative Peak Review p. 79 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM K1001011-020 3^ PFBA 1 213.0/169.0 Da Baseline c Missed n Wrnnn Peak PFBS 1 299.0/80.0 Da o Baseline c Missed r Wrnnn Peak 6.16 13C-PFBA 217.0/172.0 Da Baseline c Missed ___r Wrong Peak___ 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak PFPeA 1 263.0/219.0 Da Baseline Missed r Wrnnn Peak I PFHxA 1 313.0/269.0 Da Baseline c Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline c Missed r. Wrnnn Peak 13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Paak 6.2 ,L PFHpA 1 363.0/169.0 Da Baseline c Missed ___r Wrnnn Peak___ S_180-PFHS 403.0/103.0 Da Baseline Missed ____n Wrnnn Pnak___ 13C-PFHxA 315.0/270.0 Da Baseline Missed p Wrnnn Peak 7.^5 PFHxS 1 399.0/80.0 Da Baseline c Missed n Wrnnn Peak 7.^5 * <Oil 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak 11*6 c. .W- - :o*i T*ne. inni 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Paak______ S 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak______ 13C-PFDA 515.0/470.0 Da Baseline d Missed n Wrnnn Pfiak___ Before After Page 127 of 169 200 p. 80 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation ! 1,22 "i 3.53 PFBA2 213.0/169.0 Da g Baseline c Missed l Wrong Peak 6.16 PFPeA2 263.0/219.0 Da Baseline g Missed o Wrong Peak K1001011-020 ............ 4. 20....... t ...... PFBS2 299.0/99.0 Da c Baseline g Missed u Wrong Peak PFHxA 2 313.0/119.0 Da o Baseline u Missed Wrong Peak This inaoe is nol avaiable PFHpA2 363.0/319.0 Da Baseline c Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed g Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed a Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline o Missed Wrong Peak Page 128 o f 169 201 Created with AnalysPR%jborter Printed: 18/02/2010 3:24:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02171 OVSamplesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial KQ1000664-06 2/17/2010 11:17:36 PM PFCkinetexLong.dam 50 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 130-PFDoA PFTeA 1 13C-PFD0A IS Area 4027100 2474900 8130900 2741700 132950 8130900 146890 146890 146890 Analyte RT (Exp RT ) 1.27 (1.36) 2.07 (2.06) 2.09 (2.09) 0.00(3.06) 3.88 (4.06) 3.88 (3.87) 4.54 (4.55) 5.16 (5.06) 5.67 (5.51) Analyte Area 2836 Calc. Cone (ng/mL) 0.043 -4026-- -- o.wa-JJj) 88793 0.117 0 N/A 10619 0.044 7173 0.028 9692 ^ 2 ^ - oe r o - KP 3384 N/A S943- -r vjk -------- Oree?- rjpTT Modified? No No No No No No No No No Page 150 of 170 202 Created with A n a ly it Reporter Printed: 18/02/2010 3:24:00 PM IRC Summary I Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 0.00(1.36) 1.28(1.36) 2.04 (2.08) 0.00 (2.44) 2.10(2.12) 2.07 (2.00) 3.05(3.06) 3.06(3.04) 0.00(3.88) 3.87(3.88) 0.00(3.87) 0.00 (4.53) 4.52 (4.64) 5.14(5.13) 5.76(5.56) Area 0 4027100 2909 0 29971 8130900 322 2741700 0 132950 0 0 146890 252 65 Modified? Calc (Exp) MRM Ratio No 0.00 (2.16) Ratio Confirms ID No No 1419.94 (43.19) No No 0.28(0.34) YES No 0.00(0.00) No No 0.34 (0.32) YES No 91.57 (21.71) No No 0.00(0.14) No No 0.00(17.53) No No 0.00 (0.19) No No 12.52(1.60) No No 0.00 (0.32) No No 0.00 (0.28) No No 15.64(3.71) No No 0.07 (0.34) No No 0.01 (0.29) No Page 151 of 170 203 Created with AnalystFRforter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review 1.28 l L*A, PFOA 1 413.0/169.0 Da Baseline d Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wrnnn Peak _______ KQ1000664-06 N b 2.07 ,/V L u ,' PFNA 1 463.0/419.0 Da o Baseline Missed r Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline c Missed Wrnnn Peak PFOS 1 499.0/80.0 Da o Baseline Missed in Wrnnn Peak 3.88 A/viWl 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak 1500C llXKK' W00 PFDA 1 513.0/469.0 Da c Baseline c Missed Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed Wrnnn Pnak PFUnA 1 563.0/519.0 Da Baseline o Missed ___r Wrnnn Peak___ 4.54 PFDoA 1 613.0/569.0 Da o Baseline Missed n Wrnnn Pnak 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Pnak 4 .^ PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 20000 13C-PFOS 503.0/80.0 Da a Baseline c Missed n Wrnnn Pnak 8 ,s" 10000 J 5000 5.16 li 2*4 <0 <5 50 55 13C-PFD0A 615.0/169.0 Da Baseline Missed n Wrnnn Peak ^5 50 55 60 PFTrA 1 663.0/619.0 Da QBaseline Missed n Wrnnn Peak______ T*i*s, imn 13C-PFDOA 615.0/169.0 Da D Baseline Misse n Wrnnn Pnak .0 5.67 PFTeA 1 713.0/669.0 Da Baseline c Missed ___ r Wrnnn Pnak___ ,.L SO 55 13C-PFDoA 615.0/169.0 Da Baseline Missed ____n Wrnnn Peak___ Before After Page 152 of 170 204 Created with A n a l^ t^ p o r te r Printed: 18/02/2010 3:24:00 PM Ion Ratio Confirmation lv, ! ' ' KtiQ 2$oo a. MM | 'M'O 1000 too Tim^rmn PFO A 2 15 ta 25 Tm*mir. PFNA2 JO 4 1 3 .0 /3 6 9 .0 Da 4 6 3 .0 /2 1 9 .0 Da B aselin e M issed B a se lin e M issed W rong P eak W rong P eak KQ1000664-06 ?[iOU 000 #i,, 5000 4000 3000 A s 200 100U ....................................... - 15 .'0 25 30 ?oec * I5M f .1000 1 BOO> 3.05 PFO S2 4 9 9 .0 /9 9 .0 Da B aselin e 0 M issed W rong P eak PFDA2 5 1 3.0/26 9.0 Da B aselin e o M isse c W rong P eak PFUnA2 56 3 .0 /2 6 9 .0 D a d B aselin e M issed c W rong P eak .0000 ' PFDS2 5 9 9 .0 /9 9 .0 Da B aselin e c M issed W rong P eak % litCtOrt 400* __________ 5,76. ..... . ` 0 5 5 50 PFTeA2 7 1 3 .0 /1 6 9 .0 D a B aselin e M issed W rong P eak Before After PFDoA2 6 1 3 .0 /1 6 9 .0 Da B aselin e M issed c W rong Peak PFTrA 2 6 6 3.0/16 9.0 Da B aselin e M issed p W rong P eak Page 153 o f 170 205 Services" p. 85 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's ProjectstPFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial KQ1000664-06 2/18/2010 6:08:10 AM PFCkinetexShort.dam 50 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 130-PFHxA PFHxS1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 6007300 7104400 3859600 7104400 7104400 3859600 4447500 4447500 Analyte RT (Exp RT ) 1.24 (1.27) 3.52 (3.60) 4.18 (4.26) 5.22 (5.31) 6.16 (6.23) 6.23 (6.32) 6.22 (6.31) 7.52 (7.65) Analyte Area 58453 14790 5516 38455 10535 42021 3859600 4447500 Calc. Cone (ng/mL) 0.03 0.01 0.02 N/A N/A 0.02 1.07 1.00 Modified? No No No No No No No No 5 ^ $ iio Page 149 of 169 206 p. 86 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM (t IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.24 (1.27) 3.52(3.60) 0.00 (4.26) 0.00 (5.31) 6.13(6.22) 6.22(6.32) 1.24(1.26) 5.23 (5.30) Area 58453 14790 0 0 35089 16685 6007300 7104400 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.00 (0.40) No No 0.00(0.10) No No 3.33 (2.90) YES No 0.40(0.40) YES No 102.77(13.20) No No 184.75(13.71) No Page 150 of 169 207 Quantitative Peak Review Created with A n a lys f Reporter Printed: 18/02/2010 4:02:00 PM KQ1000664-06 5.23 PFBA 1 213.0/169.0 Da c Baseline c Missed p Wrnnn Peak PFBS 1 299.0/80.0 Da Baseline a Missed ___ p W rnnn P e a k 6.16 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da c Baseline Missed r, Wrnnn Peak PFPeA 1 263.0/219.0 Da Baseline c Missed n Wrnnn Peak i<6601 )000 i 5.22 PFHxA 1 313.0/269.0 Da Baseline Missed p Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline c Missed ___p Wrnnn Peak----- Tns_m r 13C-PFHxA 315.0/270.0 Da Baseline c Missed Wrnnn Peak PFHpA 1 363.0/169.0 Da Baseline c Missed n Wrono Peak S_180-PFHS 403.0/103.0 Da o Baseline Missed ____n Wrnnn Peak S i 70 13C-PFHXA PFHxS 1 180-PFHS 315.0/270.0 Da 399.0/80.0 Da 403.0/103.0 Da Baseline c Missed Baseline Missed Baseline Missed n Wrnnn Peak i 2*6 7.52 l 0*6 1aoes 1(.0*5 <0*5 k :os 1 70 75 80 1m mm 13C-PFDA n Wrnnn Peak 12*6 16* 5 ,0 ti 7.52 1 1 | i0#5 40*5 1 1 20*5 I00*0 OS 70 75 eo Tme nn S_13C-PFDA 1 2*6 10*6 2 10*0 n Wrnnn Ppak 7.52 1 60*5 S 46*5 :os t `. I I1 70 '5 Tn>*. 13C-PFDA 515.0/470.0 Da 515.0/470.0 Da 515.0/470.0 Da Baseline Missed Baseline Missed Baseline Missed ____n Wrnnn Peak___ r Wrnnn Peak___ ____p Wrnnn Peak___ Before After Page 151 of 169 208 Columbia Analytical Services" Ion Ratio Confirmation p. 88 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM KQ1000664-06 3.52 PFBA2 213.0/169.0 Da o Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline c Missed Wrong Peak PFHxA 2 313.0/119.0 Da c Baseline Missed g Wrong Peak 6,13 PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak Tne mr, PFHxS2 399.0/99.0 Da Baseline Missed c Wrong Peak S_13C-PFDA 515.0/470.0 Da a Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After This irnge is nof amiable N/A Baseline Missed Wrong Peak Page 152 of 169 209 Services" Created with AnalystfRorter Printed: 18/02/2010 3:24:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\SamplesLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial KQ1000664-05 LX 5 2/17/2010 11:10:00 PM PFCkinetexLong.dam 49 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3966700 2891200 9123700 2954000 148890 9123700 143000 143000 143000 Analyte RT (Exp RT ) 1.25 (1.36) 2.04 (2.06) 2.11 (2.09) 3.11 (3.06) 3.91 (4.06) 3.91 (3.87) 4.55 (4.55) 5.09 (5.06) 5.54 (5.51) Analyte Area 3324900 5813800 17391000 5525000 3840400 11353000 1897000 893190 889630 Calc. Cone (ng/mL) 16.500 18.500 17.800 18.100 24.600 11.000 21.900 18.400 24.400 Modified? No No No No No No No No No Page 146 of 170 210 Columbia Created with AnalJ^t Reporter Printed: 18/02/2010 3:24:00 PM IRC Summary I I Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.26(1.36) 1.26(1.36) 2.05 (2.08) 0.00 (2.44) 2.10(2.12) 2.11 (2.00) 3.10(3.06) 3.12(3.04) 3.90 (3.88) 3.90(3.88) 3.91 (3.87) 4.55 (4.53) 4.56 (4.64) 5.08 (5.13) 5.54(5.56) Area 7661500 3966700 1861700 0 5644000 9123700 774190 2954000 701970 148890 3701800 510120 143000 295280 337010 Modified? Calc (Exp) MRM Ratio No 2.30 (2.16) Ratio Confirms ID YES No 1.19(43.19) No No 0.32(0.34) YES No 0.00(0.00) No No 0.32(0.32) YES No 0.52 (21.71) No No 0.14(0.14) YES No 0.53(17.53) No No 0.18(0.19) YES No 0.04(1.60) No No 0.33(0.32) YES No 0.27(0.28) YES No 0.08 (3.71) No No 0.33(0.34) YES No 0.38(0.29) No Page 147 of 170 211 Quantitative Peak Review Created with AnalystPR@fiorter Printed: 18/02/2010 3:24:00 PM KQ1000664-05 PFOA 1 413.0/169.0 Da a Baseline c Missed n Wrnnn Peak 2.11 * i Cii PFOS 1 499.0/80.0 Da Baseline c Missed ..n Wrong ..Peak.. 13C-PFOA 417.0/372.0 Da Baseline o Missed n Wrong Peak 6.5 i 4#s 5 l*5 i2.11 13C-PFOS 503.0/80.0 Da Baseline o Missed r Wrong Peak PFNA 1 463.0/419.0 Da d Baseline d Missed n Wronn Peak JL_ui 3 V. | 3*i 1 !5 13C-PFNA 468.0/423.0 Da o Baseline Missed r Wrong Peak___ i3.12 PFDA 1 513.0/469.0 Da c Baseline Missed n Wrnnn Peak JL 13C-PFDA 515.0/470.0 Da c Baseline Missed n Wrong Peak L< 2.11 PFUnA 1 563.0/519.0 Da Baseline Missed n Wrnnn Peak 13C-PFUnA 565.0/270.0 Da Baseline Missed ___ n Wrong Peak PFDS 1 599.0/80.0 Da Baseline Missed n Wrong Peak___ 13C-PFOS 503.0/80.0 Da Baseline c Missed n Wrong Peak PFDoA 1 613.0/569.0 Da c Baseline Missed n Wrong Peak 13C-PFDoA 615.0/169.0 Da Baseline Missed n Wrnnn Peek PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak 130-PFDoA 615.0/169.0 Da Baseline Missed n Wrong Peak PFTeA 1 713.0/669.0 Da Baseline d Missed ____n Wrong Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed r Wrnnn Peak Before After 212 Page 148 of 170 Created with Anal^fstReporter Printed: 18/02/2010 3:24:00 PM Ion Ratio Confirmation L1.26 PFOA 2 413.0/369.0 Da Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFUnA 2 563.0/269.0 Da d Baseline Missed Wrong Peak Tim* min PFDS2 599.0/99.0 Da Baseline Missed a Wrong Peak KQ1000664-05 PFOS2 499.0/99.0 Da c Baseline c Missed Wrong Peak I 4 a. _____ jb i z PFDoA2 613.0/169.0 Da Baseline Missed o Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak LZZ-------------- 5 8 ---------- ---- PFTrA 2 663.0/169.0 Da Baseline Missed c Wrong Peak PFTeA2 713.0/169.0 Da Baseline o Missed Wrong Peak Before After Page 149 of 170 213 umfoia Created with Analyst*fcjorter Printed: 18/02/2010 4:02:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 021710\SamplesShort.wiff 021710short.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial KQ1000664-05 \jC , . 2/18/2010 5:58:36 AM PFCkinetexShort.dam 49 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5881500 6729500 3838000 6729500 6729500 3838000 4759500 4759500 Analyte RT (Exp RT ) 1.27 (1.27) 3.57 (3.60) 4.21 (4.26) 5.22 (5.31) 6.11 (6.23) 6.21 (6.32) 6.21 (6.31) 7.50 (7.65) Analyte Area 11118000 12190000 19228000 12713000 4836900 19103000 3838000 4759500 Calc. Cone (ng/mL) 18.00 19.10 15.20 19.20 20.30 16.30 1.00 1.00 Modified? No No No No No No No No 10 4 Page 145 o f 169 214 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.27(1.27) 3.57 (3.60) 4.20 (4.26) 5.23(5.31) 6.12(6.22) 6.21 (6.32) 1.27(1.26) 5.23 (5.30) Area 11118000 12190000 7921100 1216900 13757000 7940600 5881500 6729500 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.41 (0.40) YES No 0.10(0.10) YES No 2.84 (2.90) YES No 0.42 (0.40) YES No 0.53(13.20) No No 0.53(13.71) No Page 146 o f 169 215 Created with AnalystlR^sbrter Printed: 18/02/2010 4:02:00 PM Quantitative Peak Review KQ1000664-05 1 .2 7 3 .5 7 - 5*4: ' 5*6 6*6 Z0*6 ^ ' 0*6 S 60*5 I 13 8 5tS S 16*6 f- i | 3 50*5 :-eS S0*5 05 10 15 :o If M 40 46 Tn* mr> I 45 50 5T 60 pfba" i 13C-PFBA PFPeA 1 13C-PFHxA 213.0/169.0 Da 217.0/172.0 Da 263.0/219.0 Da 315.0/270.0 Da Baseline o Missed o Baseline Missed Baseline c Missed C Baseline Missed _____r Wrnnn Peak_____ _____ r Wrnnn Peak_____ n Wrnnn Peak r Wrnnn Peak 4 .2 1 6 .2 1 10*i 5 . 2 2 16*6 5 . ^ & 5, ;-*t I ; r 41 4` 50 PFBS 7 299.0/80.0 Da Baseline Missed n Wrnnn Peak ) 6 .1 1 0* % UiS * 4C|*6 -0*4 55 I so 65 ;a | 5 \ 4S S Cl,fc f 5 10*6 2*5 55 b0 65 .' 0 l 45 60 66 00 180-PFHS 403.0/103.0 Da d Baseline Missed __________ n Wrnnn Peak_____ 15*6 PFHxA 1 313.0/269.0 Da Baseline o Missed n Wrnnn Peak <6 6 . ^ 1 t 16 3 5De& & 3*6 f1 I 45 60 65 60 55 cO 66 70 PFHpAI 13C-PFHxA PFHxSI 363.0/169.0 Da 315.0/270.0 Da 399.0/80.0 Da Baseline Missed _____ n Wronn Peak_____ c Baseline Missed n Wrnnn Peak Baseline Missed n Wrnnn Peak Ke5 | 6', 4*5 1*5 I 55 60 65 T n * min S 180-PFHS 7(1 l 0*6 &,, ae5 t 60*5 i 1 40*5 ;o*s 7 .5 0 7fl ?S 80 13C-PFDA 10*6 & 84*5 80*5 IS 40*5 24*5 85 I 7D ?5 80 S 13C-PFDA 65 403.0/103.0 Da 515.0/470.0 Da 515.0/470.0 Da Baseline Missed n Wrnnn Peak q Baseline Missed __________ n Wrnnn Peak_____ Baseline Missed n Wrnnn Peak s 1Dot 3 50*5 *4 66 65 60 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak 85 IS 4*5 2*5 55 I 60 6s 70 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak 7 .^ 3 1u*t ,, 00*5 i- 60*5 1 40*5 L-a*5 70 ?5 80 8S 13C-PFDA 515.0/470.0 Da a Baseline Missed n Wrnnn Peak___ Before After Page 147 of 169 216 p. 96 Created with Analyst Reporter Printed: 18/02/2010 4:02:00 PM Ion Ratio Confirmation 1 .27 ........... "3.57 KQ1000664-05 Uf Ui .5 Z0 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline a Missed d Wrong Peak PFHxA 2 313.0/119.0 Da Baseline g Missed Wrong Peak . 6. il "*5 it>5 tM PFHpA2 363.0/319.0 Da Baseline c Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed c Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 148 of 169 217 Created with AnalystFRS^orter Printed: 18/02/2010 3:24:00 PM tf Project Data File Result Table Instrument Name Sample Name Acquisition Date Acquisition Method Injection Vial Ewan's Projects\PFCs 021710\SamplesLong.wiff 021710long.rdb API 5000 K-iPOlOi)- 7 /*3 KQ1000664-03 2/17/2010 10:54:54 PM PFCkinetexLong.dam 47 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3923500 2766800 8656800 2693100 121680 8656800 142700 142700 142700 Analyte RT (Exp RT ) 1.25 (1.36) 2.09 (2.06) 2.09 (2.09) 3.11 (3.06) 3.96 (4.06) 3.94 (3.87) 4.59 (4.55) 5.12 (5.06) 5.58 (5.51) Analyte Area 4830300 12547000 42860000 4988700 3058600 9569800 1777000 968150 733660 Calc. Cone (ng/mL) 24.300 41.700 46.300 17.900 24.000 9.700 20.600 20.000 20.200 Modified? No No No No No No No No No lio Page 138 of 170 218 Analytical Services'' Created with Analyd?'Ffporter Printed: 18/02/2010 3:24:00 PM IRC Summary I Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 SJ3C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.24(1.36) 1.23(1.36) 2.04 (2.08) 0.00 (2.44) 2.10(2.12) 2.09 (2.00) 3.14(3.06) 3.10(3.04) 3.94 (3.88) 3.95 (3.88) 3.94(3.87) 4.58 (4.53) 4.59(4.64) 5.11 (5.13) 5.58 (5.56) Area 11005000 3923500 4139200 0 14362000 8656800 757060 2693100 624660 121680 3095900 478240 142700 318000 209810 Modified? Calc (Exp) MRM Ratio No 2.28(2.16) Ratio Confirms ID YES No 0.81 (43.19) No No 0.33(0.34) YES No 0.00 (0.00) No No 0.34 (0.32) YES No 0.20 (21.71) No No 0.15(0.14) YES No 0.54(17.53) No No 0.20(0.19) YES No 0.04(1.60) No No 0.32(0.32) YES No 0.27(0.28) YES No 0.08 (3.71) No No 0.33(0.34) YES No 0.29 (0.29) YES Page 139 o f 170 219 Sendees' Quantitative Peak Review 1.23 i PFOA 1 413.0/169.0 Da Baseline u Missed r Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline c Missed r Wronn Peak | 2.09 PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak 1_ _ _ _ mL*_ _ _ _ 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak Created with AnalystBR^Sbrter Printed: 18/02/2010 3:24:00 PM KQ1000664-03 2.09 PFNA 1 1463.0/419.0 Da Baseline c Missed -i Wrnnn Peak 3.31 PFDA 1 513.0/469.0 Da Baseline c Missed Wrnnn Peak 13C-PFNA 1468.0/423.0 Da c Baseline c Missed r Wrnnn3.P1e0ak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak PFUnA 1 563.0/519.0 Da Baseline c Missed ___r Wrnnn4.P59eak PFDoA 1 613.0/569.0 Da Baseline d Missed hi Wrnnn Pnak 13C-PFUnA 565.0/270.0 Da Baseline c Missed ___ n Wrnnn Peak___ OOt-O 13C-PFDOA 615.0/169.0 Da c Baseline Missed n Wrnnn I PFDS 1 599.0/80.0 Da o Baseline c Missed __ r ,,Wrong Eaak__ -SiI_________ 5^2________ 13C-PFOS 1503.0/80.0 Da Baseline c Missed )0*4 1 n Wrnn4n.5P9 eak___ PFTrA 1 663.0/619.0 Da Baseline Missed r Wrnnn Pnak 13C-PFDoA 615.0/169.0 Da Baseline Missed n Wrnnn Pnak___ PFTeA 1 713.0/669.0 Da Baseline o Missed n Wrnnn Pnak 13C-PFD0A 615.0/169.0 Da Baseline Missed n Wrnnn Peak___ Before After 220 Page 140 of 170 Ion Ratio Confirmation Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM KQ1000664-03 Before After Page 141 of 170 221