Document MM52b8MJJgnm8Y0YQykDX11Bj

AR 226 _ 0831 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4.4,55,6,6,7,7,8,8.8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 628. The test sample is FC-600. Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl! sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hhexaminium chloride, 0.80% starch, and 0.05 % benzotriazole. - The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the the inhibition by the fluorochemical componentof the test sample. METHOD Method: 3M Protocol, Reference EAR 11/8/79 Test type: Acute static GLP: No Year Completed: 1983 Analytical monitoring: Dissolved oxygen concentrations Statistical methods: Graphed Test organism source: Activated sludge `mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN Test condition: Dilution water: Deionized water. Synthetic sewage: Not noted Reference and test solution preparation: A stock solutionofthe - test substance was created by diluting 0.60 grams of test sample to 100 mL with dilution water. Itis not noted exactly how the 1,000 mglL test solution is prepared from this stock solution. Test vessels: Not noted Number of concentrations: 1 plus 1 blank control Temperature: Not noted Total suspended solids and pH on day of testing: Not noted Element Basis: Respiration inhibition as determined by oxygen - consumption. RESULTS Nominal concentrations: Blank control and 1,000 mg/L 002016 Element values: Nreospaicruattieoninrhaitbeitaotry1e0f0f0ecmtgo/nLawcittihvaatecdonstlaucdtgetime of 4 minutes, 55 seconds. Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test SnoutbsttheanfcleuoRreocmhaermkicsaflieplrd.opoTrhteiovnaalluoener.eported applies to that mixture and CONCLUSIONS iLnihmiibtietdthceonatcaticvtittyiomfe aecsttiivmaatteedssltuhdagteF.C-600, Lot 628 is not expected to Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 3. Testing lacks record of methodology wusaesd.notThleonmgeetnhooudgwhatso dneoftinaintiavegleyndceytearpmpirnoeveifdthmeertehomdi.ghTtebset douthreartion teifmfeesc.ts tThhaet msiagmhptloecpcuurritdyuwrainsgntoyptipcarlopwearsltyecwhaatrearctterreiaztemdenatndrettheentsitoundy lacks analytical confirmationofthe amount of fluorochemical proportion in the solution. REFERENCES This study was conducted by the 3M Environmental Laboratory, St Paul, MN, Lab Request number 8875, 1983. OTHER Last changed: 6/28/00 : 002017 a TER Co TEITM s sme mri, S LE oH swawretsoe[ soner.sd28,8575e-f1(Fe-toguoreas cs EE Jai TERS ces Hil ! : i : JITITININSUITSTRTToEtve eet pee | i HINT J :I NE 1 NNT iRfE DU STR | Il I Jams RIN | CH J Si PR JTnTAnTETN NL be A By MH | i ee wiei + 3M Environmental Laboratory Acute Toxicity of a Compound (Substance) to.Activated Sludge Mixed Liquor Protocol Reference: EAR 11/8/79 raid es Jr ms cole Lob request no: _ BETS vate: 2/0/23 Analyst4: Sedat The activated sludge mixed liquor was collected from the St. Paul/Minneapolis Sample description: (FC - GOO Lo7-Fa 3 fc 2 2 = FLrR#2a27Do5|r stat gettin, Dogifl gros of semple were i heted 16 JOR I i aA rr A il ge fe ERE LL lal leans[|T 1} sf few | e 1[ l7 e Lo [=Jaeelra | || l=loa |@rop tml] le|= [78a -- I Le cr rrrrr HH ctrrr r[ t 1 7 COMMENTS: . BEST COPY AVAILABLE } 002019