Document MJzzmp2MO5prVEMbz4LBQM899
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
TEST SUBSTANCE
Identity:Mixturecontainingperfluorooctanesulfonatmea;y alsobe referred toas PFOS, FC-95, oras a component ofFC-3072. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was not noted.The testsample isFC-3072. Currentinformatioinndicateistisa mixtureof1.67% test substance,33.33% diethyleneglycolbutylether,29.23% water,20.83% urea, 5% sodium octylsulfate3,.33% sulfonatedalkylethersulfatesalts,4.60% acrylicfoamer,1.67% triethanolaminea,nd 0.33% tolyltriazole.
The followingsummary appliesto the testsample as a mixture of the testsubstance inwater solutionwithincompletely characterized concentrations of impurities.Data may not accurately relatetoxicityof the testsample with thatof the fluorochemical component.
METHOD:
Method: OECD Guideline202 Type: Acute static GLP: No Year completed: 1995 Species: Daphnia magna Supplier:In-houseculturesO.riginalloybtainedfrom U.S.EPA-ERL, Duluth, MN. Analyticalmonitoring: Temperature, pH, and dissolvedoxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticalmethods: Not applicabledue to study results. Test conditions:
Dilutionwater: Carbon-filterewdellwater (3M Well #2, St.Paul, MN). Dilutionwater chemistry: Not given.
Lighting: Not given. Stock and test solution preparation: A primarystock solutionwas prepared by dissolving1000 mg oftestsubstance in1 L water. Exposure concentrationswere preparedby additionofstocksolutiontoeach vessel. Exposure vessels: 100 mL vesselscontaining50 mL testsolution. Number of replicates:4 Number of daphnids per replicate:5 Number of concentrations: sixplusa blankcontrol Water chemistry during the study:
pH range (0-48hours): 8.3-8.3 (controelxposure)
8.1- 8.3 (1000mg/L exposure) Temperature range (0-48 hours): 20.2 - 21.6 'C Dissolved oxygen range (0-48 hours):
8.1 - 8.4 mg/L (controlexposure) 4.0 - 8.1 mg/L (1000 mg/L exposure)
RESULTS
Nominal concentrations: Blank control5,6, 100, 180, 320, 560, and 1000 mg/L.
Element values: 48-hour EC50 = >1000 mg/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on the mixtureof the testsubstance as described inthe testsubstance remarks field.The values reportedapply to thatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 48-hour EC5o was determined to be >1 000 mg/L, the highestconcentrationtested. No mortalityor sublethaleffectswere observed.
Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability:Klimischranking= 2. This study meets the criterifaorquality testing.However, sample puritywas not properlycharacterizedand the study lacks analyticaclonfirmationofthe amount offluorochemicalproportionin solution.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St.Paul, MN, Lab Request number M3597, 1995.
OTHER
Last changed: 6/25/00.
ENVIRONMENTAL LABORATORY
48-HR ACUTE TOXICITY TO DAPHNIA. DAPHNIA MAGNA
TYPE TEST:
STATIC
x STATIC RENEWAL
TEST SUBSTANCE:
FC@3072 AFFF Base
DILUTION WATER:
CARBON-FILTERED WELL WATER (3M WELL 92,ST. PAUL. MN) TEMP. 21.6 *C, pH 8.3. D.0. B.1 mg'L
TEST DAPHNID SOURCE:
USEPA-ERL. DULUTH. MN
DATERECEIVED: 628,90
DAPHNID AGE:
<24 HR OLD NEONATES
NO. OF DAPHNIDSRTEST CHAMBER:
TEST SOLUTION VOLUME:
50 mL
SIZE OF TEST CHAMBER:
100 mL
DATE INITIATED: 3Z.@ 1) 4
DATE TERMINATED:
TES VESSEL
SKI BK-) BK3 BK4
INITIAL TOTAL CONC AMOUNT mg/L ' ADDED' CONTROL
CONTROL
CONTROL
CGt4TRCL
MORTALITY
24-HR
48-HR
pH
C.
"IZ@ .3
D.O. mg/L
LR M3597 5
COMMENTS
r-nL
56
28
0-2
56
2,8
G-3
56
28
0-4
56 1 2.8
1-1
1DO
5 .0
1-2
100
50
1-3
100
so
1-4
100
5,0
2-1 2-2 2-3
2-4
3-1 3-2 3-3
3,4
4-1
180
9,0
ISO
90
ISO
9.0
180
9.0
320
16
320
16
320
16
320 1
16
560
28
4
560
28
4-3
560
28
560
28
5-1
1000
so
5-2
1000
so
5-3
1000
so
r@-4
1000
so
I
I
TEMP. c
IDATE / ANALYST
ECSO (95% C.I.)mg/L
c
C)
t)
-c)
IL
'Slock solution 1000 ma'L. Each 1.0 mL 1.0 mg.
cl, C,)
>
7-
I --I-E
q3
ANALYST