Document MJrB2GQLE1jze88MX4kyaB0RV
# AR226-2878 Copies to:
E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine
HASKELL LABORATORY REPORT NO. 397-79
Haskell N o . : 12,943
Material
_ _____________
Submitted by: Chemicals, Dyes &| Pigments Dept.
Jackson Laboratory, Chambers Works
Other Codes :
Sample Ready for Testing: 3/13/79
PRIMARY SKIN IRRITATION AND SENSITIZATION TESTS ON GUINEA PIGS
Procedure: A range-finding study was conducted with 3 male albino guinea pigs to test for skin irri tation potential. A 20% solution of the test material in water was the highest concentration tested due
8^ in irritation potential of the glacial acetic acid solvent. No skin irritation was observed at this level."
The primary irritation test was conducted on 10 unexposed guinea pigs by applying and lightlv rs>nboubiT'aSe9r insvki?n*.5 mi each f a 20% and a 2% (v/v) solution of the test material in water on shaved, intact
1 iwhe ^ndYCti?n p}138 for sensitization was a series of 4 sacral intradermal injections of 0.1 ml of a 1.0% solution in physiological saline, 1 each week beginning 2 d.ys after the test for primary irri-
. 13"? ayr?t Period* th test animals were challenged for sensitization by applying and
in -05 ml each ? f 3 20% and a 2% (v/v) solution of teat material in water on shaved, intact shoulder skin. At the sarhe time 10 unexposed guinea pigs (controls) of the same age received identical topical applications.
Results :
Concentration in water
_______ Test Animals (10 Animals - In. Avg. Wt.
%+10 Animals Pinal Avg. Wt.
20 2%+
Primary Irritation Test - 24 Hours
- 48 Houts
Challenge Test
- 24 Hours - 48 Hours
10 neg. 10 neg.
10 neg. 10 neg.
10 neg. 10 neg.
10 neg. 10 neg.
Sensitization Response Ratio** at 20% Concentration 0/10
Control Animals 401 g (10 Animals - In. Avg. Wt. 693 g) 10 Animals Final Avg. Wt.
20% J2L
368 682
10 neg. 10 neg.
10 neg. 10 neg.
g g)
Company Sanitized. Does not contain TSCA CBl
%
f'
w
2- -
#
SMMftffE1
(H-12,943) caused no irritation or sensitization when tested as a arw __j
(v/v) solution in water on the shaved, intact skin of guinea p*igs.
20* d a 2,6
No unusual dermatitis hazard is expected from the use of less in water.
at concentrations of 2096 or
CompositionI
from the same amount Spplied^initiail^or onthe Concurrent control!? over the responHe expected
i Rancre Findincr Results
i.
Solution (96)
Intact Guinea Pier Skin
20 3 Neg.
; 10
3 Neg.
12
f. 0.2
3 Neg. 3 Neg.
&Report bys
si
yanne L. T e r e h z
*7=\ Toxicologist
RLP/aph
.Date Issued Jul\
Report No
1979
Approved bys
Gerald L. Kennedy Chief, Acute Investigations
Company Sanitized, Does not contain TSCA CBl
