Document MJnoeR8bp5gZ2BN2kzmkEDwVy
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G. DAPHNIDS) TEST SUBSTANCE
Identity: Nre-fmeertrheydltpoerafsluNo-rMooecFtOanSeEsAullcfoohnoalm,idFoCe-t7h9a0n,olo;r mFMa-y3a8l2s5o,be(1Octanesulfonamide, N-methyl-1,1,2,2,3,3,4,4,5,5,6,6,7,7,8.8,8-
heptadecafluoro-N-(2-hydroxyethyl)-, CAS #24448-09-7)
Remarks:
Material number
is an were
noofft-wrheictoer,dweda.xy
solid.
Sample
purity
and
lof
METHOD
`Summary of2 Studies
Method: U.S. EPA 660/3-75-009, April, 1975.
Type: Static acute
GYeLaPr:cNomopleted: 1978 Species: Daphnia magna Aannadlydtiiscsaollvmeodnoixtyorgienngr:eNcoomridendal concentrations only. Temperature, pH
Statistical methods: Probit analysis. Concentrations tested exceed water solubility of the test substance.
TTeesstt ddaapphhnniidd saoguercate:stOubdtyaiinnietdiaftrioomn:thNeeMoongautlesCorporation.
Test conditions:
Dilution Dilution
wwaatteerr:cChaermbiosn-tfriyl:tered
well
water
Hardness*: DO:
28396mmogl/lL as CaCOs
cop*: Alkalinity*:
<1 mg/L 221 mg/L as CaCO3
CPoHn:ductivity":
48270 pmhos/cm
Total Solids*:
286 mglL
* Values taken as averages from general laboratory water quality testing in 1978.
Stock and test solution preparation: Not noted inreport.
006598
Exposure vessels: Test vessels were described as jars. Each test
vessel contained 100 mL of test solution.
NNuummbbeerr ooff dreapplhincaitdess:peornereplicate: 20 Water cDhiesmsiosltvreyd odxuryignegnt(hreecsotruddeyd: at 48 hours or at 24 hours
when all test organisms were dead):
7.8 mg/L (control exposure Test 1)
77..56 mmgg//LL ((c1o0n0t0romlge/xLpoesxuproesuTreestTe2)st 1)
8.5 mg/L (8700 mg/L exposure Test 2)
PH (recorded at
organisms were
48 hours
dead):
or
at
24
hours
when
all
test
8.6 (control exposure Test 1)
88.2.7 ((c1o0n0t0rolmge/xLpoesxuproesuTreestTe2)st 1)
Test te8m.p6e(r8a7t0u0rmegr/aLngeexp(o0s--ur4e8Theosutrs2:)
19-20C
Element basis: mortality and immobilization
Rinedmuacrekssu:ffiAcifeinrtst msotrutadlyiwtyatso caolnldowucttheedcaaltccuolnactieonntroaftainonLsCt5o0o.loTwhteo
study was repeated at higher test substance concentrations.
The test substance was noted to precipitate out gradually over the
courseof the test. It was generally noted that more precipitate formed in the higher concentration test solutions.
RESULTS
Nominal concentrations:
Testi:
One 560,
blank and and 1000
mogn/eLsolvent
control,
100,
180,
320,
Test2: 2O4n0e0,bl3a7n0k0,an5d60o0n,e8s7o0l0vemngtlcLo.ntrol, 650, 1000, 1550,
Element value:
Test Test
1 2
48-hour 48-hour
ECs ECs
(95% (95%
CI): CI):
Not calculated. 1352 (1194-1533)
mg/L
Statistical Evaluation: The ECs; value and 95% confidence intervals were calculated using probit analysis.
006599
Test 1 Percent Mortality/iImmobilization
aq 0d hid conemaimenat
[[mEeem v ev o] ]
ev v1 r Err
.
I|
ani i hil Test 2 Percent Mortality/iImmobilization ENED I c =ro a a c r]
a|
IL| cCOoNvCoLwUSmIoOnNsS
The test sample 48-hour ECs for Daphnia magna was determined to be greater than the test substance solubility limit.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
o0 mDAoTAuQUnALiITy0 Y 000 0
Reliability: Kiimisch ranking 3. These studies lack analytical data for
determinationof
determination of
the
the
test substance
sample purity.
concentration in the test solutions
The tests should have been
and
replicated. There was no descriptionofstock solutionpreparation.
Testing
with the
waaidsofalascoedtoonnee
wteolel nahbaonvcee tshoelusboilluibtiyl.ity
limitofthe
testsubstance
RReEFFEeRREENNCGEESS
00000000000000000 006600
These studies were Laboratory, 3M Lab
conducted by the Request Number
3M Company, 4197, 1978,
Environmental
O-T_HER
OOOO
Submitter: 3M Company, St. Paul, Minnesota, 55133
Environmental
Laboratory,
P.O.
Box
33331,
Last changed: 5/12/00
006601
por
TECHNICAL REPORT SUMMARY
70: TECHNICAL COMMUNICATIONS CENTER -- 201.2CN important ~ frporti pritedan bothsidesofpaper,sndtwocopesto TCC.)
Jan. 8, 1979
Environmental Laboratory Fate of Fluorochemical Project Aquatic Toxicity Studies: Fu 3925 D. L. Bacon
A. N. EWeelter
"0535 9670012600
pony
E09S36e2 e
i TR
eevwonr os:ra om 3m CunENT oBECTIVE:
LT
Seni Pegorad
Dotti Sugoonother Tsounfdiestherm(Lienpeomitshemaaccrouctheir9u6s )haonudr LtChq8,048valhuoeur fLoCr, bluveagliulel
EE & PC Div
for Daphnia magna exposed to varying concentrati8ns of
(LEanbv)ironmental | FM 3925.
)(TAoqxuiactiitcy)
oSTTARSETRA,[20350ve Thedc iran avormdyhTeoGomenion or
Tdehfeinliitmiivteeddweatteerrminsaotliuobniliotfytohfe FaqMua3t9i2c5 ptorxoihciibtiytedofa
tLhCigsq tveasltuesmateexrcieaeld. theBotwhatevrertseoblruabtileitayndofinFvMert39e2b5r.ate
.
ic: DR.. A. PRriockkoerp RW.TL.. EBlonhaobnar: AEl. A. MReenidneelr 5. K. Welsh
intErmaioLn is)ion
-
006602
a x .
_-
CONCLUSION
)
Tdheeterlmiimniatteidonwaotferthseoluabqiulaittiyc otfoxFiMcit3y92o5fprtohhiisbittesetd maatdeerfiianli.tive
sBootlhubivleirttyebroafteFMan3d925i.nvertebrate LCg, values exceed the water
INTRODUCTION
pTehrefotresmtedsounbsttahnicsemaFtMer3i9a2l5 eisvaalnuaotfefd-wihtsiteaqsuoaltiidc. toTxeisctitsy and the data obtained form the basis of this report.
METHODS
A." Aquatic Testing_
.
mTohdeelteesdtapfrtoetroctohlast udetsiclriizbeeddfobry UtSh/eEsePAst(u1d9i75e)s. were
.
B. Acclimation Procedure
Tfhreomblauepgriivlaltesunhfaitschher(yLtep.amieStmoaccrkocfhiirsuhs)wewrereeheolbdtaiinneda
wfeilblerwgalatsesr hatold1i4n-1g5fCa.nk Afidlalieldywipthhotocpaerrbioond-foiflte16redhours
)
1piegrhitodanwdas8mahionurtsaindeadrktwhirtohughaou3t0-mtihenutaecctlriamenfsiiotnionand
tfoeosdtinbgeipnegriwoidt.hhelTdhe48fihshourwsereprifoedr Tteotraan-dintOhroudagihloyu,t
tphreiortestto pteersitoidn.g. Fish were acclimated for 14 days
.
!
oAnn FaMcut3e925s.horCta-rtbeornm-f(i9l6t-ehrouerd)wesltlatwiactebrioaofssakynowwnascpoemrpfoosirtmieodn was used as the diluent (Attachment 1). All glass
;:
oarqudairliuaen(t35pxl2u0sx20toxcmi)canctonctoamipnrinigsed16alsittuedrys ocfhamdbielru.ent
Tatweenatcyhblcuheegmiilclalsucnofnicsehn,trautniiofno.rm Tienstsizfei,shwwereeretersatneddohly
taolxlioccaanttedatdodietaicohn.tesBtluecghialmlbersuwniftihsihnex3p0ermiimneunttess wfeorlelowing
ocxoyngdeuncteldevealt a20n-d21pH Co.f alMlorttaelsittys,oluttemipoenrsatwuerree,medaissusroeldved
Gaetne2r4a-lhouorbseirnvtaetrivoanlss orrelautnitviel ttootablehamvoirotraallitychahnadgesoccurred.
were similarly recorded when appropriate.
uThseesdeinstutdhiisesswtuedrye wpeerreforcmoendsidienrerdepltiocabtee.genOerrgaallnyisms healthy and free of disease.
`
AThperiDlap1h9n,iam1a97g8nafruosmedthien MtohgiuslsCtourdpy.weTreestopbrtoationceodlonused ~~ 006603
was that described by USEPA (1975).
* Dale Fattig Fish Hatchery, Brady, Nebraska rrmmawnial Fish fand of known composition
0 To .
_3-
J RESULTS
The bluegill sunfish (Lepomis macrochirus) used in this study
.
had an average weight of 0.5 g and length of 4.0 cms.
The loading factor for these studies was approximately
-
0.63 g/1, bejow `the maximum recommended loading factor,
of 0.8 g/1 . Test. temperatures ranged from 18 - 20C,
pH varied from 7.8 - 8.2 pH units and oxygen saturation
at 96 hours in the individual experiments were 34 and 56%.
sIninctehistherseeplviaclauteesd wstouudlyd, exLCc,88dvtahleueswawteerresonloutbiclailtcyulaltiemidts
of FM 3925. (Attachment 2).
Thg Daphnia magna
19C. pH range
osftu8dy.2wa-s 8.c7onadnudctaedmiantimaaltem4p8erhaotuurreoxoyfgen
|
ssoaltuubrialtiitoyn ooff F7M9%.3025P.rojec(tAtetdacLhCgm, dftV3a)l.ues exceeded the water
REFERENCES The data reported herein are based on studies performed
by M. T. Elnabarayy-and L. H. Pritchard.
1. US/EPA - Methods for Acute Toxicity Test with Fish
Macro-invertebrates and Amphibrians, Ecological
)
Research Series 660/3-75-009, 1975.
006604
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ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY DATA SHEET
vi Higa
2
.
ATTACHMENT #2
J. TotYoSh pan o veteriat Tweed2 IM 025
"testorgenion SBlueead disly Ave. Ve.gramAvse. Site 4 a Tost Solution Volume _\, liters/Task No. Organisms por doso____20
Edin Hohn n dee 2 Time Started__2.10D0aPtWe Started___4:-\_\D-e1t%e Completed, 4-\S 8 DilutionNater CarbonFilter - WellMatar (Towp. JS C, 0.0.89ppm, p_13) TavestigatorWET fanal, atuny LC 50 (95% Confidence Limits)
Jones] 2010 hoar]l 0[ea]soll 0Te eo] Jae]
der 20I0 oslesll [asfeal] 0hslaoll [reso]
[all20]o Teall oTeale Tlolae]l1 |otlcr]
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[ze] 20]Tule] 0fuslesll 2 fasles[|2[os]co]
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.
ENVIRONMENTAL ENGINEERING LABORATORY
AQUATIC TOXICITY DATA SHEET
`Hi7 *
ATTACMENT #2
) typo TesQtlp: Wr. dale
Material Tested FC- 392.5
Test Organiswll\. Seg Sal A Ave. We.QSpremAvsg. Size_Y ca
Test Solution Volume__ Jl, Liters/Tank No. Organisms per dose__ 00
;
Tine Started 3.30PW Date Started M4-\ NY Date Complotsd__4.- 22-79"
Dilution Water_Carbos Filter - Well Water (Tewp.)"]C.s 0.0.39ppm, wR1
tmvestigator_ERuANAN ic 50 (95% Contidonce Limite)
li ale elon al sl aeIo haleflo Msfai[l\ halal 1 fils
Sl oo 11 hslesl[ ahalal] a helsall 3Jaalscl
sl noI Diglsll 1Tusladdl Vhalal 1Taafad]
fo
aRolsall oTsohn[l ohalasll 0 [292d]
Lao Lg halss]l 0hahull ohaball = nlzd
alloc] ohdiall 1 Jeckoll vReal[29i ]aol
olal
/ halerfl a Maks"3 halagll =
Pglz7|-
alloc | 4heleall )TSE) hfs] 1Tmalzal :
J IN NN
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pe Ji[g 118 [" | 20% |
meats:\) 508
aS od oad
ov cock con
mlacu arli
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andro
daveDreina: 130 wa Jd and 2ag ogy 4 Ae png, a)
ers
\ ahah a AN 2 A AN Wwg.
Ws vena no
A A&
oo
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006607
SPUR
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ENVIRONMENTAL ENGINEERING LABORATORY
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AQUATIC TOXICITY DATA SHEET
) iypo TesDtiaWeghinMiaggos MaterialTestedE230(5 3)
Test orgmniaDngonaWoops Ave. ._dcgmm Ave. Site
ca
Test Solution Volume)OX vo) iorsRR No. Organisms per dose_ | aC
Time Started .'OGPr- Date Started NAN\Y Date Compleced_Y ANY
Dilution Water_Catbon Filter - Moll Water (Tesp.\A C,, 0.0.3) pom, wi3)
ediee nded tavestigator) Yu NVoh 1c 50 (95% Confidence Limits) MR Inaoarauwy
5. EP IE
YC I
I
XionJo [1 ToRehall TT1 T1T7
heolloeToTT My deeb] TTT 111
delleelol Tl oleshad TTT TT
baal na [a|oHHHHH
JeeollenIoTT TMohah Te T ll 17
oso inJo TTMalsa Tl TnT aT l
Cr1 Tr rrr Tr
oh
(10 TT T7011
e. 1ae Jlve TT
Comments:
HF Noo hiie Wocua wes te Nhe Ned yer
Cine
Seam nea Cog on Nant aS
taeonoNen an Smee NS,
STOOP AVAILABLE -- ----------------------------e--r-- r---- ------
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ENVIRONMENTAL ENGINEERING LABORATORY
ATTACRENT #3, |
AQUATIC TOXICITY DATA SHEET
| ove TestiDaghuiaYragua, Material Tested2EY2N9085wal
TestorgansTosoWage, Ave. Wegrams Avg. Size Se ca
Test Solution Volume 00 wm)aves Toh No. Organisms per dose___"00
Timo Started 3.06%, Date Started MWY Date Completed N =a Y
Dilucion Mater_Casbon Filter - Nell Mater (Tewp2C.Q0.0.33pou, #311
vestigated Bedard LC 50 (85% Confidence Linits) 135% vyJA( nav-rcas)
TM B\uedavany
+
od =. 24 Hrs, Lo o ss 0.0.72 tira, 0.0. low, oe]
RSelllaoaTFoo[[7To0 feabafellllT TTL TT] sollacloTT MTofesl[Tz T JeIl T1l]
sell sug
P a0eTaoop hleely F Fa H Td TTTlOJe TT 1]]T
:
asl no Tao helo ETT T
awarlelmo|Iao0B2ek [2H0e]eFIT7
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ENVIRONMENTALRESEARGMLAD. -- DULUTH
"sPRODIT ANALYSIS FOR DIDASSAY DATRess
NHTOOH. OTOFFDCUCAORTNVGEA.S-C7FDOR1NMGCOU..RVEEXP1OS-E7D,4NO.DEAD
77 7
611303000,35202002,50041.,2
.
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20s
c1o0c6. . FRERSAPCOTNIDOINNG
T1o3o3n0
233..811920931332 o0[.323
`+`c2h4i0-0so PRMBILI3T.Y38012S11> 0.03 1
.
wIoTEoReATIONS TintemcesT sTivae
6
24.7904
7.917801
( ITERDGEWIETY FACTOR Ob = 1
(co50 =
1391.882
3933 PPCCTT.. ULOPVPEERR CCOONNEE.. LLIIMMIITT ==
MN BN NE
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.
i
FFRIATCTTEIDON. WD ephnite> Wags
000.:6018040593%3210904%57"74-
SEE !
05737903
2
LCIHNIEASROITFY.OR PCWRIOBASBGILFOIRT.Y 1.61074 0.4465226 6 = 0.1348014 - 113313.90891 .53
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-
006610