Document MJnb3oMROjDwgMrGaomL7OE5y
AR 226 _ O/H
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also be OrceftearnreesdutlofoansicPFacOiSd,) (FCC-A9S5#, 2o7r9a5s-3a9-c3o)m.ponent of FC-206CE. (1-
Remarks: The 3M production lot number was 502. The test sample is FC-
206CE PFOS,
(125/%16/d8i9e)t.hyCluernreegnltycionlfobrumtayltieotnheirn,di7c6a.te0s9i%tiwsaatemri,x4tu%reuorfea,
0.62% 1.28%
mSuolntoooncetyflopahmeenry,l1e.t5he5r%, s0o.d0i6u%msooctdyilusmullfaautrey,l 1su.l0fa5t%e,poalnydox0y.e0t5h%ylteonleytriazole.
cThhaerafcotlelrowiiznegdscounmcmeantrryataipopnlsioesftiompaurmiitxiteusr.eDwaittah imnacyonmoplteatcecluyrately reflect the toxicityofthe fluorochemical component of the test sample.
METHOD
Method: OECD 209
TGeLsPt:tyNpoe: Acute static Year Completed: 1990 Analytical monitoring: Dissolved oxygen concentrations, pH and tSetamtpiesrtaitcuarlem.ethods: Rests were determined by calculationofthe % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro Waste Treatment Plant, St. Paul, MN Test condition:
Dilution water: Millipore Mili-QTM water Synthetic sewage: per OECD method Rreeffeerreenncceesuabnsdtatnecset,s3o,l5u-tdiiocnhlporreoppahreanotli,onw:asA sptreopcakrseodlubtyiodniosfsotlhveing 500 mg in 10mL 1N NaOH, diluted to 30mL with Milipore Mili-QTM waantderd,iltuhteend tboro1uLgwhitthtoMtihlelp-oQiTMntwoafteirn.cipTihenet pprHoecfiptihtaetiroenfewritehncNe soHl;utSiOo4n tweasst vmeesasseulsreadndtowbaes7a.p2.parTeensttlysumbisstcaibnlceewwiathswaadtedre.d directly to the Test vessels: Not given. Nbluamnbk ecorntorfolcsoncentrations: three plus 1 reference substance and 2 = TToetmaplersautsupreen:de1d7s-o1l9idsCand pH on dayoftesting: 7.853 g/L (TSS), iwnaitsial3.p1H4 6g.l7L,ainnditifailnpalHp6H.76,.9f.inaIlniptiHal6T.8S,Sainnd1M0L00SSmg3/.L34cogn/Lc.entration
oe1764
Element Basis: consumption.
Respiration inhibition
as determined
by oxygen
Remarks: Values were corrected to 20 C for calculations.
RESULTS
Nanodmi1n0a0l0cmogn/cLentrations: 2 Blank controls, reference substance, 320, 560 Element values: 30-minute ECso= >1000 mg/L.
3-hour ECs = >1000 mg/L
Element values based on nominal concentrations.
Remarks: Substance
RTeesmtairnkgswfaiseldc.onTdhuectveadluoens
arempiorxtteduarapsepldyetsocrtihbaetdmiinxttuhreeTaensdt
not
the fluorochemical proportion alone.
CONCLUSIONS
`The FC-206CE 3-hour ECso for determined to be >1000 mg/L.
activated
sludge
respiration
inhibition
was
Submitter: 3M Paul, Minnesota,
C5o5m1p3a3ny,
Environmental
Laboratory,
P.O.
Box
33331,
St.
DATA QUALITY
HReolwieavbielri,tys:amKpiilmeispucrhitryanwkaisngn2o.t pTreospteirnlgymceheartasctalelrcirzietderiaanfdorthqeuasltituydytelsatcinkgs.
analytical solution.
confirmationofthe
amountof
fluorochemical
proportion
in
the.
REFERENCES
This study Paul, MN,
was Lab
RceoqnudeuscttendumbbyetrheH314M80C-o2m,pa19n9y0,.
Environmental
Laboratory,
St
OTHER
Last changed: 6/26/00
001765
. ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
RHIMBOZ,
Date3/25/40
Analy
TypeTestActivatedSludgeRespication lnbibitionTes:OECNzm
Source Metypy waste, [reabment Plagt St Pa
Date Obtatned 3px [ap
Date Used
Ma.
x27 Jap
Mixed Liquor Suspended Solids (MLSS) MLBS3 g/L Adjustment
g/L
PH tial (0, Pinal 1,9
Comments
--
Test Substance
:
Sample
Sample
DeWsacgreispt.ion
PRwral 67]
C2fi0naolnl. oFfa|latBoSo0Am2djgus(2cmeonjtea)
CommenteLiD of LooQangl chestscin yinitialAegisSnal(Ri)2,2 gl
Reference Substance
Sample DesWcarhiepstitocnauxdeSxebi gmp)org
Sample PH jwiigl (9 0al 4.0
*Adjustment,
Coments_Sicoiksolution pi v1.2
Exposure Conditions Tea Duration 5Jou
Coments Test Temperature |" --[q hi 3
=
_-- -
*
0017BY66
Sample Description
Environmental La--b Work Sheet
. OECD ACTIVATED SLUDGE
RESPIRATION INHIBITION TEST #209
Lab RequestNo. LLINEN-2,
"0 ------ of
Date: _2/22/90
-
a Analyst: =a
Test Conc. mg added ml water ml syn, sewage ml microbial
2
1 20 + 2.84
+
"so Slo)
280
+
+
2za8L4
+
+
BxGatmlI Oo + 284 +
o
4+
2.00
la
+ 200
+ 200
3 + 200
Shared BkContvolTCwith wweo-d
-_
an
cq
Total volume
= S500 = 500
= woo
= son
--__-- 0
- _--
B
-_--
---
-_
--
eee eee reeseeee
eee
oO
0x76
ENVIRONMENTAL LABORATORY
*
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance:F'C. 200 CF,
Source of Activated Sludge
boot.
Mixed
502
Liquor:
zl) 0] 84.
MebynWaste
Treatment
Plast, Si
Paul,Me
Date Received:
2/22/q
Date Used: _3/23 [4
Anatyst:Zohel FAD --
eeIt Ta, 30 - MINUTE EXPOSURE
TTT
os
[vo
DISSOLVED
Joa(mo fos
fOXiYGoENTaCOsNCTENuTRoATIOfNSasmofofaa
[f
CONTROL 1
2
3.8
o
oi
HE Ee 000
pr
Nepal1 C1 |
EH
selscluzluolalsialliza laolaBnBsY
3 - HOUR EXPOSURE est substance
DISSOLVED OXYGEWNE-MCIONNUCTEEN:TRATIONS mg Tt
CONTROL
a
2
0
9
8
0
0<
ooo
SEEEAREEEns llaanallsoen[luaarlb s se ilasl h[izaali (zfohoe aslBoREs EN
FREI Dc
CONTROL IT
[og ea lit ma male zl lzlos]
PH of Activated Sludge Mixed Liquor: Initial w.7
Final 64
TpHesotfTehmipgehreastturceo:nce1n1t-r1at9ionC.tested:| Initial LT
Final 4.8
001768
ENV LKUNMENIAL LABORATORY
ACTIVATED SLUDGECRAELCSUPLIARTAITOINONOFINRHEISBUILTTISON TEST #209
Test SubstanceBC200oCE hobSZ(zjiglea)
IR HINE0-2,
Ris Analys;
30 - MINUTE EXPOSURE
ConcTeesnttraStuibosntance(ng/L) CONTRO
0
a0
000
Ovengen Utpptake Rate RtoC2o0rpCected&) + RIenshpiiibbriitatitiioonniRnate (2)
Loma
1 ham
1 | o woeg o | n gai m | 7 <
x
TT
CONTRO
[we 1a mmr|
Percent Difference in Controls! 48%
PoeRy
BCs (95% Confidence Limits) mg/l
l BUPOSR
ConTceesnttrSautbisotnan(cneg/L)
con
0 a0
000
jen Uptake Rate
R m Corrected'
%
| ama | zs |
| omel | \iage |
| [vov ter e | |d eppeea y ||
-
ep Inhibition in Respiration Rate(?)
Q
ome
CONTRO
[| own | ioe |
Percent Difference in Contr5o7t%sTM__ Ist
Es (95% Confidence Limits) ee
______
@) Tew. Correctionz --LPL (ng 0,/1-min.)
(3) 4Differences o------2x 10
os
RC; + RC,
'
RC, (2) % Inhibition=:
+ RC. 4
X 100
The tuo, controlrespirationrates
should be within 15% of each other
R=- Orygenn uptakeuptakerarattee ((m.g O,/LL-min.)
T,obs =Sampietemp. C
6 == C1.o0n4t7r,o "x = T,obs -
2 20C
i.
*Rete
rot.calculable;
O%Y3en
depleted
too
rapidly,
Ww.
less
.
thaw
001769
one wrivute.
Wry 3`,5SaDmipolentDeosrcaripphteiornol, 4%
Telomnee vi bot 7,00
Environmental Lab -- Work Sheet
OECD ACTIVATED SLUDGE
RESPIRATION INHIBITION TEST #209 Stock and Test Solutions Preparation
Lab Request No. HI480
720 --1-- of 5 oat: L5/25/90 [ oe ax SE
-_--
Test Conc. ng/L
10
ml stock soln Der, 500 m1
mM) water ml Syn. sewage per, 500 ml feedper 500 ml
10
+2 +,
+ 200 = sa ml microbial inoculumDer S00 mL
Total volume nl
I SE
-_-- _-- -_-- _-- -_-- -_-- -_ -_ -_ --- ---
-_--
eeti0
ENVIRONMENTAL LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 RAK DATA
Page rvs
TSeosutrcesuobfstaAncctei:va5te,dS=SlDuNdgiecMhilxeodmpUieqnuoorl:M(Reemle\rnlmaaskcee,WrSautbesnteanvtpPel)ant.SEPaul,My
Date Received: m/z /an
hysti Zarlelle Fides
Date Used: Z/zzfqp~~
30 - MINUTE EXPOSURE
scarEStTratSiuobsntaoncge )fos {1.0Jus [2DIS0SOLVED2sOX[YiGEoRNE1-H0ICNO0GNCTENTaRATIOJNSasnJg on fon 7|
hh 0
CL29 T| loo |T Io 8 T old T a
I
CT TTT
I mon alsaluslualasfliazTias[loaa24s]
3 - HOUR EXPOSURE
ConceESnTtratSuibosntafneceast)
: DISSOLVED OXYGEM CONCENTRATIONS m-o
MEHINUTE
T
A a
L b OT l o8 ole |lo2 |T 10.0]5.8
erie1H] dev Ava
EH como. 11 [se[uo [uu[xalaa fo [7aTn[plone]
TPBeHHstooffTehAmicpgteihrveaastttuerdceo:nScl1eu8nd-tgr1ea9tM0iiCox,nedteLsitqeudo:r: IInniittiiaall ((5.8
FFiinnaalll1o..0S
-
001771
.
ENVIRONMENTAL. LABORATORY
Test Substance 5,ASCTcIDViAcTEhDloSLrUoDgGEhCeARLECSUPLI(ARRTAITeOIeONNvOeFINdRHEIcSBeUILTSTISuONbTsEkSTan#2e09)
RT 30 - MINUTE EXPOSURE Test Substance
Oxygen Uptake Rate
R Co ted a)
CONTRO!
0
[| omoemed T| ap guese|
IR HIRO Be+te z
PS 3
% Solidi " : Inhibition in
8
CONTRO
| vizar 1 ean
Percent Difference in Control(s) Bg (95% Confidence Linits) mgt
N%
0000
dQ EXEOSE
ConTceesnttrSautbisotnan(cneg/L)
CORO
Oxygen URptake Rate
RtoCo20rrCected!) % RIenshpiibriattiioonniRnate(?
[te |m o5pa
0
|oseon |asia]
CONTRO
| omer |an]
PHegrcenOtSDifCfoenrfeindceenceinLiComnittreo)ls(m)g_/__l 527% :
) Temp.Correctionz--LaL (ng 0,/L-nin.)
oF
2
(3) Difference=--L dy 100 RC, + RC.
Et) (2) % Inhibitions.
+ RC.
X 100
The two control respiraztion rates
should be within 15% of each other
Rhe=reO:xygen uptake rate (ng2 O,/L-nin.)
8T',=o=b1sC.o0n=4t7rS,oalmp"lxet=eTm,p0,bsC-
ch 20C
:
001772
3MEnvironmentalLaboratory -RawDataSheet
bower
i errr mSeAT RR mens x
ir ERI T=
oh,[ren[en[i[HE |i |mee|
2
oe
------ sp poses Lus nLooacmms | aumsoo {meSscsommnsal]
T pe e o p eam n aee Pa o |
Tt I --
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