Document MJjRGZjKBDpxERR0YR3zeZNj9

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST ellm'zTAK'rl= ldenfity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredto as PFOS, FC-95, oras a component ofFC-3003. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notgiven.The testsample isFC-3003. Currentinformationindicatesitisa mixtureof 1% PFOS, 15% diethyleneglycolbutylether,79.39% water,2% Sultonefoanier,1.5% sodium oetylsulfate1,% sodium decylsulfate0,.06% sodium laurylsulfatea,nd 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the ffuorochemical component of the testsample. METHOD: Method: OECD Guideline. Type: Acute staticrange-findinsgtudy GLP: No Year completed: 1997 Species: Pimephalespromelas Supplier:Aquabc Biosystems,Inc.F,ortCollinsC,O Analyticalmonitoring: Temperature,pH, and DO. Exposure period:96-hours Statisticamlethods: Not given. Test fishage: Juveniles(128-132davs) Length and weight: Average length= 23 mm Average weight= 0.09786 g (wet) Loading: 0.2936 g/L Pretreatment: None Test conditions: Dilutionwater: Carbon-filterweedllwater(3M Well #2, St.Paul,MN). Dilutionwater chemistry: Not given. Lighting:Not given. Stock and testsolutionpreparation:The testmaterialwas added directly tothe dilutiownater on a weight/volumebasis. Concentrations dosing rate:Once. Stabilitoyf the testchemical solutions: Not noted. Exposure vessels: 1.2L vesselscontaining1.0L testsolution. Number of replicates:One, Bk controlduplicated Number of fishper replicate:3. Number of concentrations: threeplus2 blankcontrols Water chemistry during the study: pH range(0-96hours): 7.9- 8.2(contreoxlposure) 7.7- 7.9(1000 mg/L exposure) Temperature range (0-96hours): 18.5OC Dissolved oxygen range (0-96hours): 7.8- 8.5mg/L (controelxposure) 4.5- 8.6mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control,10,100,and 1000 mg/L Elementvalues: 96-hourLC50=<1000mg/Lbut>100mg/L Element values based on nominal concentrations Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hour LC50 forfatheadminnow was determined tobe <1000 mg/L. The 1000 mg/L exposure caused 66% mortalitwyhilethe 100 mg/L exposure had no mortality. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking= 3. The study isa range-findingw,ithlimited exposure concentrationsand testorganisms. Sample puritywas notproperly characterizedand the studylacksanalyticaclonfirmationofthe amount of fluorochemicalproportioninthe solution. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number R1331, 1997. OTHER Last changed: 6/27/00 ENVIRONMENTAL LABORATORY 04 ACUTE TONCITY TO FATHEAD MINNOW, PINERiolLES PROMELAS LR kk33 i PO I of 2- TYPE TEST-. STATIC X STATIC RENEWAL 9 TEST SUBSTANCE: DILUTION WATER* Fr-- :5oo zs CAFOON-RLTERED Temp._ A F-FP CA r.,rLcpt,) - WELL WATER ON WELL 02,ST. PAUL, MN) OCPH ,MM _mg/L TEST FISH SOURCE: un;(. C- '6,e,,'5146@e vrl,.S DATE RECTIVED- 9-19-97 FIA/ RSH AGE: f3::L NO. OF FISKITEST CHAMBER: AVG. WT.:b.oq78(@. 0 0 3 LOADING RATE: STZE.- ;Z --S 0, TEST SOLUTION VOLUME: 1,04- SIZE OF TEST VESSELS: TEST VESSEL is DATE MMTED: INITIAL TOTAL CONC. AMOUN1 Y"O mgk ADOT D 6C.4r- L4- 1'4 -cl"l Li.OC).AV" -vv--s 24-M PH 1 0.0. 48-HR o 17.1 0 Fo-I 1-9 W-1 0 DATE TERMINATED. L@l MORTALITY . PH 060.1724IRI pH MOIL 7,q 0 1IQ 1-9 o cl -1 D.O. 9644R norl 1!9-5- pH D.O. MOIL V-1 '1-9 '1.14 A 10 to 4isi 1 1 t,7- 0 1,9 c> -7.ct -I,o ".,0 1R,D 7,b 0 1 7-o 100. )C>O '4P, 1 7,'t II.-iI q,S I :P, 1 7.41 17.3 1 9- 1 -7 q 15 .,,T TEMP. OC 18-50 ,DATE / ANALYST L4-15 -Q'l Ikoo -Tk-s q. -1 -7 -TK-5 q7 -cl 1-@9r LCSO (96% C.I.) Iocc) L COMMENTS SECONDARYEF FECTS: S Swbbbg; E Lossd EqulWum; 0 OWOOOOM -7 L4 d.-.. Jn 'lot; 9-ec.\o t fJG R EffaftSwMwWng; H Hemoft00rV ANALYST: Environmental Laboratory LR: P, I3a I Date: @t-it-9-1 lnitials:---Vks Page'IL.of; W3@-HourAcute ToxicityTo Fa#md Loading Rate Date Sh"t Test Substance: rc- 3ooz Minnow, Pimephalot Promoiss LI Fish I 2 3 4 5 6 7 10 Average Welcht, (a) o. 091(a 0.05-42- o-12-S,3 Lanoth,fem) .1-44 1 .1? a-5 0.0?lz 0,09147- 0.06(ol 0. 0 (0laa 0. o ct(0,4 O-z of .9 o -9-1 ir(, .1.0 7-.7- 2.(10 al.3 Landine Rate Calculation Avg. wL (g.): 0. 09-7,?6 Std. Dev: 0.0 4iG 9 No. fish/vessel: 3 Vol./vessel: 0. a9 35 g. fish Loading rate: ;Z