Document MJZxV2d1nzyOrLGgM5xZbexbj

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, oras a component of FC-206C and L-5439. (1-Octanesulfoniaccid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafploutoarsos-i,um salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was 501. The testsample is L-5439. Currentinformatioinndicatesitisa mixtureof0.73% PFOS, 76.09% water,15% diethyleneglycolbutylether,4% urea,1.55% sodium octylsulfate1,.47% hydroxyfoamer, 1.05% polyoxyethylene monooctylphenylether,0.06% sodium laurylsulfatea,nd 0.05% benzotriazole.. The followingsummary applies toa mixture with incompletely characterizedconcentration of impurities.Data may not accurately reflectthe toxicityof the fluorochemical compnent of the test sample.. METHOD: Method: 3M Protocol,Reference EAR 11/8/79 Test type: Staticacute GLP: No Year Completed: 1981 Analyticalmonitoring: Dissolvedoxygen concentrations Statisticamlethods: Graphed Test organism source: Activatedsludgemixed liquorcollectefdromthe MetroWastewater TreatmentPlant,St.Paul,MN Test condition: Dilutionwater:Deionizedwater. Syntheticsewage: Not used Reference and testsolutionpreparation:A stocksolutioonfthe testsubstance was createdby dilutng0.60 grams oftestsample to 100 mL withdilutiownater. Itisnot notedexactlyhow the 1,000 mg/L testsolutonispreparedfromthisstocksolution. Test vessels: Not noted Number of concentrations:1 plus 1 blankcontrol Temperature: Not noted Totalsuspended solidsand pH on day of testing:Not noted Element Basis: Respirationinhibitioans determined by oxygen consumption. RESULTS Nominal concentrations: Blank controland 1,000 nig/L Element values: No acute inhibitoreyffecton acbvated sludge respiratiornateat 1000 nig/Lwitha contacttimeof6 minutes. Element valuesbased on nominalconcentrations. Remarks: Testingwas conducted on the mixtureas describedinthetest substance remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance exhibitedno inhibitoreyffectsat1000 mg/L over a contacttimeof6 minutes. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksrecordof methodology used. The method was notan agency-approved method. Test duration was not longenough to definitivedleytermineiftheremightbe other adverse effectsthatmightoccurduringtypicalwastewater treatment retentiotnimes. Sample puritywas notcharacterizeadnd the studylacks analyticaclonfirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES Thisstudywas conducted by the 3M EnvironmentalLaboratory,St Paul, MN, Lab Request number 6812S, 1981. OTHER Last changed: 6/26/00 L-AAJ:(t,?tZS SLUDGE TOX. RESULTS I- Ll IL z C:) LLJ f>>--<'3 CD LLJ C> Li 2 3M Environmental Laboratory (Acute Toxicity of a Compound to Activated Sludge Mixed Liquor) Sample Description: I -5rq,3@C?Ct3lll-.26 Analyst: ZJ.4. Date Analyzed: 7-ol-el Lab Request No.: 6elz *9 4or- 7 -ITf 4- -------- - - H-d Of. T114E - minutes 10 12 Acute Toxicity 3M Environmental Laboratory of a Compound (Substance) to.-Activated Sludge Protocol Reference: EAR 11/8/79 Mixed Liquor Lab reques t no. Analyst: The activated sludge mixed liquor was collected from the St. Paul/Minneapolis Metro Wastewater Plant on 7- Of - AtI - Sample description:-- F(,(n4 Z!27---.J-O Preparation of stock solution: 0.(oO with deionized grams of Sample were diluted to IOC> reagent water. Date 07'Proparation:--2-0/-R/ ml TIME . min. sec 30 ONCENT 1O.N O@OJEST CshTi6e 1 &mq9@/1 1 mg/i -7@.ro 7,30 71 ts7 ASTI mq/1 lcbn ol ED SL m GE )IBRE 9! mq COMENTS: /,;zo O)cr6EA)PRO&' To A &JAr Repo Si, OAJ L SAW T@i 7YC W-6 7-A6f- P,#Pj CeW 41 do rime - m*ooereg TI- 7i