Document MJ0V1YKNN57gq5b4JzBon5Vna

AR.A3.6 - 07^6 A C T IV A T E D S L U D G E R E S P IR A T IO N IN H IBITIO N T E S T T E S T S U B S T A N C E ___________________________________________________ Identity: A mixture containing perfluorooctanesulfonate; m ay a lso be referred to a s P F O S , FC-95, or a s a com ponent of F C -2 0 3 C and L-5440. (1-Octanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, potassium salt, C A S # 2795-39-3) R e m arks: The 3M production lot num ber w as 501. The test sam ple is L-5440. Current information indicates it is a mixture of 1 .4 7 % test substance, 6 0 % water, 3 0 % diethylene glycol butyl ether, 3 % sodium octyl sulfate, 3 % hydroxy foamer, 1 0 % polyoxyethylene m onooctylphenyl ether, 0 .1 2 % sodium lauryl sulfate, and 0 .0 5 % benzotriazol. The following summary applies to the test sample as a mixture o f the test substance in water solution with incompletely characterized concentration o f impurities. Data may not accurately relate toxicity o f the test sample with that o f the fluorochemical component. M E T H O D :____________________________________________________________ M ethod: 3M Protocol, Reference E A R 11/8/79 T e st type: Static acute GLP: No Y ear Com pleted: 1981 A n a lytical m onitoring: D issolved oxygen concentrations Statistical m ethods: Graphed T e st o rg a n ism so u rc e : Activated sludge mixed liquor collected from the Metro W astew ater Treatment Plant, St. Paul, M N T e st condition: D ilution w ater: Deionized water. Syn thetic sew age: Not used. R eference a n d te st so lu tio n preparation: A stock solution of the test substance w as created by diluting 0.60 gram s of test sam ple to 100 mL with dilution water. It is not noted exactly how the 1,000 mg/L test solution is prepared from this stock solution. T e st v e sse ls: Not noted N um b er o f c o n ce n tra tio n s: 1 plus 1 blank control Tem perature: Not noted Total su sp e n d e d s o lid s an d pH o n d a y o f te stin g: Not noted Elem ent B a s is : Respiration inhibition a s determined by oxygen consum ption. 001051 R ESU LT S N om inal con ce n tration s: Blank control and 1,000 mg/L Elem ent valu e s: N o acute inhibitory effect on activated sludge respiration rate at 1000 mg/L with a contact time of 6 minutes. Element values based on nominal concentrations. R e m arks: Testing w as conducted on the mixture of the test substance a s described in the test substance rem arks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________ ________________ The test material exhibited no inhibitory effect a f1 0 0 0 mg/L over a 6minute exposure. Subm itter: 3M Com pany, Environm ental Laboratory, P.O. B ox 33331, St. Paul, Minnesota, 55133 D A T A Q U A LIT Y ______________________________________________________ Reliability: Klim isch ranking 3. Testing lacks record of m ethodology used. The method w as not an agency approved method. T e st duration w as not long enough to definitively determine if there might be other adverse effects that might occur during typical wastewater treatment retention times. Sam ple purity w as not characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S _______________________________________________________ T his study w as conducted by the 3M Environm ental Laboratory, S t Paul, MN, Lab Request number 6811S, 1981. O T H E R _____________________________________________________________ L a st ch an ge d : 6/26/00 001052 SLUDGE TOX. RESULTS 001053 9 8 7 6 5 h 3 2 ] O 3M EnvironmentaI Laboratory Acute T o x i c i t y of a Compound (Su bstance) to .A c t iv a t e d Sludge Mixed Liquor Protocol Reference: EAR 11/8/79 Lab request no.: Date; 7 ~ 0 ! -- Analyst: & The activated sludge mixed liquor was collected from the St. Paul/Minneapolis Metro Wastewater Plant on ~7-- O* - 6 1 Sample description; _ tilLf-Z8 y F U *Z y L o T - ^ Q / Preparation of stock solution; O, GO grams of sample were diluted to /00 ml with deionized reagent water. Date o f "preparation;. TIME min. sec. wmg/j1r S r Ai^?SLW 6E 30 /-- 7 ,yo /.Sr* y.ir 1. o*> 6,7*" -- - - X -- C,,OQ g~.jL0 J .. s" (p _7_ -- i.o s ' 1 .8 Jr /.US' 3*2-* tf.sT 2 SC * 7 A9o "V i /0 if COMMENTS: A3^ 0.8S ' <^1055