Document MGx9ze2MJ43EJ2noL12kO61oy

5555^ 55555! AR226-2781 FOR DU PONT USE ONLY Copies Co E. I. du Font de Nemours sad Co., Inc. Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P. 0. Box 50, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 323-82 0 Material Tested Octanoic acid, pentadecafluoro-, ammonium salt* Haskell No. 12,037 Other Codes Study Inidated/Completed 2/11/82 - 3/4/82 Material Submitted by VCR&D Department Haskell Laboratory MOUSE FEEDING STUDY - 21 DAYS aSummary: T j R S B H B H B S B S E B S B S B B B was fed to male and female Crl:CD-l mice at 8*close levels (from 0.01 ppm o 30 ppm) for 21 consecutive** days. Livers were significantly heavier at 30 ppm, slightly heavier at 3 ppm and appeared normal at 10 ppm in male and female mice. No changes in liver weight were seen at feeding levels of 1 ppm or lower and the significance of the change observed at 3 ppm, in light of no differences at 10 ppm, is questionable. The only clinical sign observed was sporadic weight loss. Procedure: Forty-five male and 45 female albino Crl:CD-l mice (ages 41 and 42 days, weighing 21-33 g) were grouped by body weight, 5 animals per groj|p, 1 Uby computerized randomization. The mice were housed individually and fed (suspended in 1% corn oil before being mixed in ground Purina Lab Chow)) ror 21 consecutive days at dose levels of either 30, 10, 3, 1, 0.3, 0.1, 0.03 or 0.01 ppm. An additional group of male and female mice served as controls and received ground Purina Lab Chow only. Individual body weights were recorded weekly, clinical signs daily and liver weights at the 21-day sacrifice. 1 - TSC&CBI SanitMd. Doe not coata'n Compaq Results: Kale Mice Dose Level ppm 0 30 10 3 1 0.3 0.1 0.03 0.01 Body Wt(g) 1 Week 30 32 30 31 31 32 32 30 33 Body Wt(g) 2 Weeks 32 34 32 34 32 34 34 33 35 Body Wt(g> 3 Weeks 33 33 33 34 33 34 35 33 36 Average liver Wfeigtats (g) 1.82 4.61*** 1.87 2.45 1.89 1.83 1.82 1.91 2.07 Average Liver wt./ Body Wt. Ratio (g/100 g body wt.) 5.4 13.9*** 5.6 7.1 5.8 5.3 5.2 5.9 5.8 Female Mice Dose Level PPm 0 30 10 . 3 1 0.3 0.1 0.03 0.01 Body Wt(g) 1 Week 24 26 23 26 25 24 26 24 25 Body Wt(g) 2 Weeks 26 27 25 26 26 26 26 25 26 Body wt(g) 3 Weeks 27 26 26 28 28 27 27 27 26 Average liver Weights (g) 1.40 3.44*** 1.34 1.85*** 1.46 1.37 1.20 1.37 1.19 Average Liver wt./ Body Wt. Ratio (g/100 g body wt.) 5.2 13.3*** 5.1 6.7*** 5.3 5.0 4.4 5.0 4.5 CAS Registry Nb.:| ** Mice were mistakenly fed ground chow (without compound) for 2 days. *** Statistically different than controls, p <0.05 by Dunnett test and LSD. -2 - SanWxed. Does ,,otc<.n'"TSCACBt C o m P anV JEH:jrg:WP:1.17 .Date Issued : Ma\ Report 323-82 Approved by : J ^ L ^ S L l ^ f )d------^1 V Gerald L. Kennedy J. J]r. Section Supervisor Acute Investigations 1982 3 - ,, t s c aa CSI lized-Ooes notconia'1' Sani Company