Document MGQOmGj6Z4wMxd1J2YkKQx5M7
ACUTE TOXICITY TO FISH
ARW W
TEST SUBSTANCE
Identity: Perfluorooctanesulfonyl fluoride; may also be referred to as POSF, or FX-8. (1-Octanesulfonyl fluoride, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, CAS #30735-7).
Remarks: The test substance is a clear liquid of uncharacterized purity. It is described as FX-8 (cc842-2) Lot 1 F-10101, L-1407 washed sample.
METHOD
Method: Not given Type: Static acute GLP: No Year completed: 1984 Species: Pimphales promelas Supplier: Dale Fattig, Brady, Nebraska. Analytical monitoring: Temperature, pH and dissolved oxygen. Test substance concentration not measured. Exposure period: 96-hours Statistical methods: Not needed. Test fish age: Not noted. Length and weight: Average length = 3.88 cm.
Average weight = 0.366 g Loading: 0.61 g fish / L Pretreatment: None
Test conditions:
Dilution water: Carbon-filtered well water, 3M well #2, St. Paul,
MN
Dilution water chemistry:
Dissolved Oxygen: 9.2 mg/L
hardness:
240 mg/L as CaC0 3
pH: 8.2
Stock and test solution preparation: The test concentrations were prepared by transferring appropriate weights of the test substance directly to the test chambers. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: One gallon wide-mouth glass jars containing 3L of test solution. Number of replicates: Two Number offish per replicate: Five Number of concentrations: Four plus a negative control
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Water chemistry during the study: Dissolved oxygen range (0 - 96 hours): 6.6 - 9.2 mg/L (control exposure) 5.2 - 9.2 mg/L (1000 mg/L exposure) pH range (0 - 96 hours) 8.0 - 8.2 (control exposure) 7.8-8.2 (1000 mg/L exposure) Test temperature: ranged 21-22C
RESULTS
Nominal concentrations: Bk control, 10, 100, 500, 1000 mg/L Element value: 24-hour LC50 = > 1000 mg/L
48-hour LC50 = > 1000 mg/L 96-hour LC50 = > 1000 mg/L
All element values based on nominal concentrations
Statistical evaluation of mortality: Not applicable. No mortality seen in the any concentration tested.
Sublethal effects: After 48, 72, and 96-hours of exposure, some fish were surfacing at each concentration tested, but not in the controls.
CONCLUSIONS
The test sample 96-hour LC50 for fathead minnow was determined to be > 1000 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA Q U A L I T Y ________________________
Reliability: Klimisch ranking 2. This study lacks analytical data for determination of the test substance concentration in the test solutions and determination of the sample purity. It is estimated that this study was run in excess of water solubility.
REFERENCES
This study was conducted by the 3M Environmental Laboratory, St Paul, MN, Lab Request Number B1229-2
OTHER
Last changed: 5/18/00
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ENVIRONMENTAL LABORATORY CHEMICAL ENVIRONMENTAL EFFECTS EVALUATION
TOXICITY TEST: SUMMARY REPORT
Chemical Tested
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(Exposure/Test Conditions)
Exposure Type:
Static
Static/Continuous Orbital Shaking
Static Recirculatory_
Static Renewal
Flow-Through
Diluent:
Aerated Well Water (Initial D.O.^ . ^ j ng/l? pH < R .Zi Temp.
C(^?fiF ) )
Water Hardness: z //o
ppm Hardness Classification: '-fJf& y/D ________
Dissolved Oxygen % Saturation:
48-hr
96-hr
Extended Exposure
Control(s) Lowest Experimental Dose 1 Highest Experimental Dose pH Levels:
Control(s) Lowest Experimental Dose I Q ^ l f
Highest Experimental Dose \OOOwcjJl
Test Temperature C (F):
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48-hr
96-hr
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00662
Photoperiod: Sixteen hours light with a 15-30 minute transition period.
8EST COPY AVAILABLE ENVIRONMENTAL LABORATORY
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Comments:
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ENVIRONMENTAL LABORATORY
AOUATIC TOXICITY DATA SHEET
EXPLORATORY (RANGE-fINDING TEST)
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/ l, grans Avg. Siza^gfiCM No. Organisms per dose
Test Solution Volume 3 Liters/A ll-G lass Tank Tank Dimensions:WIDE-MOUTH 1 GALLON GLASS JAR
Time Started
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Comments:_______________________________ _________________ '
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4- 12-84
ENVIRONMENTAL LABORATORY STATUS REPORT ALL RESULTS TENTATIVE
LAB REQUEST MO. B1229
REQUESTER NAME: ANW/EAR DEDT : 0535 PROJECT NO: 9970012600 DATE RECEIVED: 2-21-84 DSSC: FX-8 (CC 942-2)
CONTRACT LAB:
CONTRACT LAB COST:
0
3 M E-L HOURS:
46
EST CMPLT DATE: 5-01-84
DATE COMPLETED:
SAMPLE NUMBER TWO WILL BE GENERATED FROM TWO EQUAL VOLUME WASHES OF SAMPLE NUMBER ONE.
SAMPLE
DATE
CODE
DESCRIPTION
TEST
RESULT
1 FX-8
SMPL-PREP
COMPLETE
2
WASHED SAMPLE
96-HR-LC50 #>1000 MG/L
C-BOD-05
SEE REPORT
C-B0D-10
SEE REPORT
C - B O D - 20
SEE REPORT
SOLUBILITY
BIOSORP
SLUDGE-TQX
# = UPDATED DATA * = CONTRACT LAB
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