Document MGDyJKpDN4j2G895GMVqyw9N7

f On February 2, 1943 we reoeived froo Ovens Illinois Gina a Co., twfvaanples of a new produot fleeignat?^ as Hydrous CaToium Silicate' for **a say of physiological hazard of Inhalation of Inhaled dust. They were labeled respectively Sander Dust* and "Rotoclone Duet.* l: ; Preliminary inspection Indicated that they were irlxtures of parti culate and fibrous natcriale, the latter having the characteristics or asbestos. A combined petrographic anu X-ray diffraction analysis re vealed the, follovir.g approzia^te compositions: ROTCfLOU" DUST Quartz Calclte X-TIW i2.> tif prrr.fCv-\?ric AP??,oxiK<?n si7?! r?c. "20 To 6 to Of nicrons. Little lecc than 1C. 10 to 20 Altered (by host?) Glaarl Chryactile (Asbestos) * ^~ r -50 Fev single fibres lec: then SO cl crone here less th.fi/. 20 r.icrr.rs. !:ost of th eii:,7le fibres mere then ICO aicrons. Uuch in unopened L-indlec. Hydr-ited Ca Silicnte w* -- Dlatonite Trace s.v;o-: D'J"T Quartz 7.5,? 5 to 10% 6 to 120 nicrone Calcite 3 10 Altered Fibres Chrysotile 25 Vood 20 to 4C Olnilar to that from rotoclone Coarse.natted together Hydrated Ca'-Silicate 25 to35? * -- , 3 0CG17 AKIKAL ASSAYS JL The naterinl as received was too coarse to be injected into avails and had to be reduoed to proper particle size. _ Grinding was not feasible owing to the presence of ehrysotile whoae structure and irritating properties are destroyed by prolonged grinding. ve vert therefore compelled to reduce it by prolonged cutting vith scissors, sieving through a 200 neah screen and adjusting the proportion of fibree and particulrte natter in the product to that of the original pecinane. Portions of eoch sacple were node up in physiological salt solution in strengths of 5 and lo fer guinea pig injection. The pH of the rotoclone sample vac 7.5 which should cause no tissue irritcticr.. That of the aander dust vs 5 which is too alkaline to be veil tolerated. The 10? suspensions were injected introperitcneally, a single dose of 2 o.c. ie. 500 sg of uust. The 5* eusper.sionc were administered directly Into the lungs by tracheal oathster* 5 dcses of 0.5 c.o each at intervale of C daya, a total of 75 ug of uust. Intranarltoneal injections of neither sample vers well received; 60 of those treated with the rotoclone sumple died within 2 to 3 weeks and 80# of the ones getting the sender duct were dead in 0 day's. All these pigs shoved aoute Inflammatory changes about n&cses of dead oelle where large accusulatlons of the diet had localised. The .early result could not be evaluated except for the fact that it never occurs after the injection of Quarts or asbestos alone. However, there were 3 ' animal that survived for 6 nonths, 2 receiving rotoclone dust and one, >ander dust. In neither of then vas there any suggestion of slllcoeie on gross or microscopic sranlnation. An aebestotlo ohange is not < ^.'*04 0 AKlHAL assays contd reoognlaable-outside of the lungs and henoe would not bfe expeotai In th$* group. *^ " -* ~ Intratracheal Injections were eonevhat 'better tolerated parti cularly in the case of the rotoclone aaaple where there were only C detthfl aaor.g C t.nivils ini these occurred 6 to 10 nonths after oon- pleting the injection..*. Of the eander dust anitala 5 died of acute reaction during the period of injection, presuaably on account of the high alkalinity. One was killed 1 aonth b:\. another 4 Months after coapleting the injections. . Both samples produced fibroLic ''ithin end about the terminal bronchioles ir. all ar.l:;nlc surviviaj fx* 1 Month or u:#rc.. T;;is reaction vas generally oonpprsble to tV t observed on injecting pure ckrysotlle asbestos, lyuicf.l asbertneit bodies were present in si.all numbers and the diat; ibuti*?:. of tb? leaionr- v:r typical of injection asbettoeis. The only difference van a tendency for the fiirouc liicut to oe tore dense one. s-it hy*li:.i?e*' rfic-r 1 to 1C C/*i fccnthc. This effect le probably attributable to the quarts in the Mixture. nothing suggestive of silicotic noduler fibrosis could be discovered either within the lungs or the associated tracheobronchial lyc;.r. nco-es. Suauary: After further reduction in size the two a&uples of this mixture of asbestos, wood fibre, quarts and hydrated calciua silicate ieitoxlc on Intraperitoneal and intratracheal injection into guinea pigs. The high alkalinity of the saucer dust probably accounts for the earlier deaths in the ease of intraperitoneal injection where a large dose is concentrated in a localised cavity and absorption la rapid. However, the deferred deaths froa the rotoclone saaple with a pH of only 7,5 sug gests cone other injurious property. 5 'OiS (3) _ ASIMAL ASSAYS CfT*E The chronio effects in the few eninale surviving for a nonth or Bon^are.Apparently duo to tLe-fibrouc coaponents of the dust and tht nature Indicates that asbestos le responsible. This is eosevhat r codified either by previous treatment of water or by the associated q but there it nothing cv^e-stive of silicotic nodular fibrcsic. If thi hydrous o&tclur. cilicate hae any part in the picture such port is not recognisable. Whether sanufacture cr use ci this product vculd ever reduce particle else te the extent th>t v: i have done is outside ci the proTin of thir stud;*. If 11 chi..Id a pots ntial hasard vo:*!;. be created, Kov ever, study of the tv;- set-piee cuoi.itlrd everts t.'u.t host cf the asbestos fibre is either vary lens or ii coar'oeed cf unopened bundles fibre, neither cm of diich is readily inhi.li.ble. nw* - . *? t' U* i <AC4y. , ** ^ Sir enter, Saranac labors r^-CY3