Document MG6yJR16m9GQkEEQaGqLmMEZa

3M ENVIRONMENTAL LABORATORY. METHOD FLEUXOTRROACCHTEIMOINCOAFLPCOOTAMSPSOIUUNMDPSEFRRFOLMUOLRIOVOECRTFAONREASNUALLFYOSNIASTUESOIRNGOHTPHLERC- ELECTROSPRAY/MASS SPECTROMETRY Method Number: ETS-8-6.0 Author: Lisa Clemen, Robert Wynne Adoption Date: 021 Ll1g Revision Date: ~~ NK Approved By: Laboratory Manager ' Sito fr Group Leader 1/2 fo Date PU1t189 Date Tx A Dore `Technical Reviewer OF19[94 Date 1.0 SCOPE AND APPLICATION 1.1 Scope: This method is for the extractionofpotassium perfluorooctanesulfonate (PFOS) or other fluorochemical compounds from iver. 1.2 Applicable Compounds: Fluorochemical surfactants or other fluorinated compounds. 1.3 Matrices: validation Rabbit, report. rat, bovine, and monkey livers or other tissues as designated in the Word 6095 ExtractiEonToSf.P8FO6S0from Liver 001021Page lofis 2.0 SUMMARY OF METHOD 2:1 r(TehPairgsoesnmt)etaohnrododmtehdetershcfyrlilubtoeersrotct-hhbeeutmpyirlcoaecltehdseuurrrf(eaVcfItoBarEn)etx.strfIarncottmihnilsgivmperoe,ttoahsosdoi,tuhsmeerpveetrinfsslfuuleousro,orouocsctihanengmciaecnalffoonnaptaeiring psgautrtatrniadtcaitroednd.e;dAPniRnOtiooSn,MIpPaBiFErO.iSngAT,rheePagFMeOIntSBAEisAae,xdtdEre(adcFtOtioSstEth-reaOnsHsa,fmeprPrlFeedOaStnoEdAat,cheeMnt5arn3ia6fl,uytaenfdonsupmaiorgaaistlse can bu fniilttreorgedenthervoaupogrhaato3rcucntpillasdtriyc. sEyraicnhgeexattrtaaccthiesdrte0c.o2ns0t.2ituutmedniynlo1.0nmfIilt.egremietnttuohbaegnlaoanlsdsfpaouuettnovoinatlos,a 22 `Tmheetshoedss.ample extracts are analyzed following method ETS--7.0 or other appropriate 3.0 DervTIONS 31 PFOS: perfluorooctanesulfonate (anionofpotassium salt) C,F,SO; 32 PFOSA: perfluorooctane sulfonylamide C,F,,SO,NH, 33 PFOSAA: perfluorooctane sulfonylamido (cthyl)acetate CF,SO,N(CH,CH,)CH,CO, 3:4 CEitF F1O;SSOE,-NO(HC:H2,(CN-Hc,t)hyClHp,erCfHl,uo0rHooctane sulfonamido)-cthyl alcohol 3:5 PFOSEA: perfluorooctane sulfonyl ethylamide C.F,SO;N(CH,CH,H 3.6 MSS6: C,F,,SO,N(H)(CH,COOH) 3.7 Surrogate standard: 1H-1H-2H-2H perfluorooctane sulfonic acid 4401 WaHemawlmthesanadnSoaCfeatvy mWaornnsings: 41.1 `Uhsaendulniinvgearsnailmaplretciasusutei,onwsh,iecshpemcaiayllcyonltaabionraptaotrhyocgoeantss., goggles, and gloves when 5.0 INTERFERENCES 5.1 There are no interferences known at this time. 6.0 6.1 EQuipvent The following equipment is used while performing this method. em ---- Equivalent equipment is acceptable. 6.1.1 6.12 Ultra-Turrax T25 Grinder for grinding liver samples Vortex mixer, VWR, Vortex Genie 2 6.13 Centrifuge, Mistral 1000 or IEC 6.1.4 Shaker, Eberbach or VWR ExtactonEoTfSP8F.O6S0fiom Liver 001022 Page 20614 6.1.5 Nitrogen Evaporator,Organomation 6.1.6 Balance (sensitivity to 0.1008) 20 71 Suppuies Gloves Axo MaTERIALS 7.2 Dissecting scalpels 7.3 Eppendorf or disposable pipettes 7.4 Nalgene bottles, capable of holding 250 mL and 1 L. 7.5 Volumetric flasks, glass, type A 7.6 I-CHEM vials, 40 mL glass 7.7 Plastic sampule vials, Wheaton, 6 mL (or appropriate size) 7.8 Centrifuge tubes, polypropylene, 15 mL 7.9 Labels 715 Timer 7.10 Oxford Dispenso--r3.0to 10.0 ml 7.11 Syringes, capableof measuring 5 uL to 50 uL 7.12 Graduated pipettes 7.13 Syringes, disposable plastic, 3 cc 7.14 Syringe filters, nylon, 0.2 ym, 25 mm 7.16 Crimp cap autovials and caps 7.17 Crimpers Note: Po rior wtaotuesri.ngRignlsaessswyarriengaensdabmotitnleis,murimnsoe f93 ttiimmeesswwiitthhmmeetthhaannooll,a3ndri3nsteismefsro`wmit3hsMeiplalria-te 8.0 REAGENTS AND STANDARDS 8.1 Typel reagent grade water, Milli-QTM or equivalent; all water used in this `method should be Milli-QTM water and be providedby aMilli-Q TOC PlusTMsystem 82 Sodium hydroxide (NaOH), J.T Baker or`equivalent 83 Tetrabutylammonium hydrogen sulfate(TBA), Kodak or equivalent 84 Sodium carbonate (N&,CO,), 1.T. Baker or equivalent 85 Sodium bicarbonate (NGHCO,), 1. Baker or equivalent 8:6 Methyl-tert-butyl ether, Omnisolv, glass distilled or HPLC grade 8.7 Methanol, Omnisolv, glass distilled or HPLCgrade 88 Liver, frozen from supplier 89 Dry ice from supplier 8.10 Fluorochemical standards 8.10.1 PFOS (3M Specialty Chemical Division), molecular weight = 538 BumcimorrroShom Lv 001023 MH 8.102 PFOSA (3M Specialty Chemical Division), molecular weight = 499 8.10.3 PFOSAA (3M Specialty Chemical Division), molecular weight = 585. 8.10.4 EIFOSE-OH (3M Specialty Chemical Division), molecular weight = 570 8.10.5 PFOSEA (3M Specialty Chemical Division), molecular weight = 527 8.10.6 M56 (3M Specialty Chemical Division), molecular weight = 557 8.10.7 CSuJrFr,o,gSaOt,eHs)tamnodlaercdu:l4a-rHw,eipgerhftl=uo4r2oo8ctane sulfonic acid (1-H, 1-H, 2-H, 2-H 8.108 Other fluorochemicals, as appropriate 811 Reagent preparation NprOepTaEra:tioWnh,eadnjupsrtepaacrciorndginlagrlgye.r volumes than listed in reagent, standard, or surrogate 8.11.1 11000N0 smoLdibuemakheyrdcroonxtiadieni(nNgaOSH0)0:mWLeiMgilhlaip-pQrTMoxwiamtaetr,elmyi2x0u0ntgilNaalOl Hs.oliPdosuarreinto a dissolved. Storeina 1 L Nalgene bottle. 8.11.2 110NNsoNdaiOuHm hsyoldurtoixoindein(oNaaOH1)0:0 mDLilvuotleum1et0NriNcaflOaHsk 1a:n1d0.diMluetaestuorveo1l0ummeLuosfing Milli-QTM water. Store in a 125 mL Nalgene bottle. 811.3 0of5TMBAteitnrtoab2ut1 yLlavmomlounmeiturmihcycdornotgaeinnisnuglf5a0t0e (mTLBAM)i:lliW-eQTMighwaapteprr.oxAidmjautsetlyto169 oPfNHa1O0Hus,inagddapsplroowxliymabteeclayus4e4tthoe5p4HmcLhaonfge1s0aNbrNupatlOy)H. (WDihliulteeatdodvionglutmhee lwaistthmL Milli-QTM water. Store.ina 1 L Nalgene bottle. 8.11.3.1 TneBeAderdequusiirnegs1aNchNecakOpHrisoorltuotieoanc.huseto ensure pH = 10. Adjust as 8.11.4 a0.p2p5roMximsaotdeiluym2c6a.r5bgoonfatseo/dsioudimumcabribcoanrabtoena(tNea,buCfOf,e)r a(nNda,2C1O.0y/gNoafHCsOo,d)i:umWeigh bQiTMcarwbaotneart.e S(tNoraeHiCnOa,1 iLntNoaalg1 eLnevoblotutmleet.ric flask and bring to volume with Milli- 812 Standards preparation 8.12.1 Prepare PFOS standards for the standard curve. 8.12.2 Pfrleupoarroechoetmhiecralflsutoarnodcahredmsiacraelasctcaenpdtaardbsl,e a(fsoarpeprxoapmrpilatee,.onMeulwtoirckoimnpgosnteanntdard s1o.l1u0tpiopnmcoEnttFaiOnSiEn-gO1H..0)0 ppm PFOS, 1.02 ppm PFOSA, 0.987 ppm PFOSAA, and 8.12.3 Weigh approximately the actual weight. 100mg of PFOS into a 100 mL volumetric flask and record 8.12.4 Bring to volume with methanol foar stock standardofapproximately 1000 ppm (ng/mL). 8.12.5 aDiplpurtoexitmheatsetloyck50soplpumt.ion with methanol foar working standard 1 solution of ExtractioEnoTfSPF8O.S60from Liver 001024Pagedof 14 8.12.6 aDpiplruotxe.th5e.0stpopcmk.solution with methanol foar working standard 2 solution of 8.12.7 Daiplpurtoex.th0e.5s0topcpkm.solution with methanol for working standard 3 solution of 813 Surrogate stock standard preparation 8.13.1 WeCiFg,h,SapOp,rHoxiinmtaotael5y05m0l-6v0olmugmetorfiscurfrloagskataensdtraencdoarrdd t1h-eH,a1c-tuHa,l2w-eHi,gh2t-,H, 8.13.2 Bring to ppm. volume with methanol for a surrogate stockof approximately 1000-1200 8.13.3 Prsetpoacrketaosaur1r0ogmaltevowlourmkeitnrgicstfalnadsakradn.dTbrrainnsgfetor vapoplruomxeimwaittehlmye1t.h0anmoiloffsourrarogate working standard of 10-20 ppm. Record the actual volume transferred. 9.0 SAMPLE HANDLING 9.1 All samples are received frozen and must be kept frozen until the extraction is performed. 10.0 QuALITY ConTRoL 10.1 Matrix blanks and method blanks 10.1.1 An aliquot of 1.0 mL methanol is used as asolvent blank. 10.1.2 aEsxtmreatcthotdwobl1a.n0kms.LaliquotsofMilli-QTM water following this procedure and use 10.1.3 Easxtmraatcrtitxwbola1n.k0s.mLReafleirqutoots1o1.f1l.i6.ver homogenate following this procedure and use 102 Matrix spikes 10.2.1 Pthreepaacrceuraancdyoanfatlhyezeexmtartarcitxiosn.pike and matrix spike duplicate samples to determine 10.2.2 rPerceepiavreedewaicthhsepaickhe susaimnpglea sseat.mple chosen by the analyst, usually a control liver 10.2.3 AEdxdpietcitoendalcosnpcieknetsramtaiyonbsewiilnlclfuadleldinatnhdemmaidy-rfaalnlgienoftthehleowin-irtaianlgceoalfitbrhaetiionnitciaulrve. calibration curve. 10.2.4 mPrienpiarmeumonofe 2matmraitxrisxpiskpeikaensdpmeartbraitxchs.pike duplicate per 40 samples, with a 103 Continuing calibration verifications 10.3.1 iPnrietipaalrcealciobnrtaitniuoinncgucravlei.bration verification samples to ensure the accuracy of the 10.3.2 oPrfe1p0arsea,mpalte2s.minFiormuemx,amopnlee,coinfatisnuaimnpglecasleitbr=at3i4o,nfvoeurrifviecraitfiiocnatsiaomnpslaerepperregpraoruepd and extracted. ETS860 Extractionof PFOS from Liver Page Sof14 001025 103.3 pPrreeppaarree tchaechinictoinatlicnuuriven,g calibration verification from the same matrix used to 10.3.4 cTahleiberxatpieocntecdurvceo.nceAndtdriattiioonnaslwsiplikfeasllmwaiythibne the mid-rangeof the initial included that fall in the low-range of otnhelyintihtieallocawliebnrdatoifonthceurcvael.ibrTahtiisoniscunrevcees(sfaorryeixfatmhpelea,na5lypsptbmu--s1t0q0uapnptbi,tartaethuesring than 5 ppb -- 1000ppb). 1.0 CALIBRATION AND STANDARDIZATION 1L1 Prepare matrix calibration standards 11.1.1 `WmeLisgMhialplpir-oQxTMimwaatteelr.y G4r0ignodftloiavehroimnotgoean2e5o0usmsLolNutailogn.ene bottle containing 200 11.1.2 140 g ratio. is not available, use appropriate amountsofliver and water to ensure a 1:5 11.1.3 cRoenfceern0tra1t3i.o0notfoscoallicudlaltievetrhteisascuteuadlisdpeenrssietdyoifnl1i.v0ermhLomofohgoemnoagteenaantdethsoelution. 11.1.5 sAhdadki1ngmbLeotfweheonmoalgieqnuaottsewthoiale1p5rmepLarcienngtraitfoutgaelotfucbie.ghRtee-esuns|pemnLd saolliuqtuiootns boyf homogeneous solution in 15 mL centrifuge tubes. 11.16 Two 1 mL aliquots, or other appropriate volume, serve as matrix blanks. 11.1.7 tThyepiecnadlolfytuhsiestsheectsitoann,datordspcioknec,enitnrdautpiloincsataen,dtswpoiksitnagndaamroducnutrsvelsi,stfedoarintToatballeof1, at eighteen samples, two matrix blanks, and two method blanks. 11.1.8 `RwehfiecrhtloisvtasltihdeatwioornkrienpgorrtasnEgTeSs-a8n-d6.t0heaLnidneEaTrSC-a8l-i7b.ra0t-iVo-n1RoarngAtet(aLcChRm)enftorB, calibration curves. 11.19 sUtsaendAatrdtsa.chRmeefnerttCo a1s3.a0ntaoicdalincuclaaltceulaacttuianlg ctohnecceonntcreanttiroantsioofnsPofFtOhSeiwnocrakliibnrgation standards. 11.2 sTuorreoagcahtewowrokriknigngstsatnadnadradr,dbfloarnkt,heorcocnocnetnitnruaitnigonvetroiffialclatwiiotnh,iandtdheapcparloipbrriataitoenacmuoruvnetraonfge 5 ppb -- 1000ppb. ExtractioEnToSfP8F:O6S0from Liver 001026 Page of 14 11.3 sExttarnadcatrdsspitokeedstlaibvleirshhoemaocgheninaittieasl cfuorlvleowoinngth1e2m.a14s-s12s.p2ec5torofmtehtiesr.method. Use these Table 1 Approximate Spiking Amounts for Calibration Standards Working Standard (Approx. Conc.) ul `Approx. final conc. of PFOS in liver 050 ppm 00..5500 ppppmm [4TT o5.002i5opppmm 030ppm 00pm | [4 20 | 0 000.5000pppmm [Sopm [To | 020mm [50pm T7720 00.570500ppppmm CSo|m 4 m i00pm 12.0 ProcepuRe 12.1 Obtain frozen liver samples. 122 Cut approximately 1 performed quickly, g of liver using a not allowing the dissecting scalpel. liver to thaw. This paor fthte procedure is best 123 Weigh the sample directly into a tared plastic sampule vial. 12.4 Record the liver weight in the study notebook. 12.5 Return unused liver portions to freezer. 12.6 Add 2.5 mLs ofwater to sampule vial. 12.7 Grind until the the sample. sample Put the grinder probe is homogeneous. in the sample and grind for about 2 minutes, or 12.8 Rinse the probe into the sample with 2.5 mLs water using a pipette. 12.9 Take the grinder apart and clean it with methanol after each sample. Refer to AMDT-EP22. 12.10 Cap the sample and vortex for 15 seconds. Label the sampule vial with the study number, `weight, liver ID, date and analyst initials. . ExtractionoEfrsPsF.OS60from Liver 001027 Page7014 12.11 vciPeainlp.tertRitefeufge1er.0ttuombeLa.t,tLaoacrbhoeetldhetwrhoerakcpsephnrteorpeirtfiuafgtoere tdvuoobcleuummweei,tnhtoiftnhhgeotimdhoeengtreiencmaaaltieinniifnnotgrosmtaaetp1si5,onm2Ls ptohelysparmoppuylleene 12.12 mPeitpehtotde btlwaonks|.mL aliquotsof Milli-QTM water to centrifuge tubes. These will serve as 12.13 sStpainkdaeradllassadmepslcersi,beidncilnusdeicntgibonla1n1k.s2.and standards ready for extraction with surrogate 12.14 Spike ofthat each matir section, for twhietchatlhiberaaptipornopcruiravtee amountof standards. sAtlasnodaprrdepaasrdeesmcartiribxedsipnik1e1s.1a,nodr Table | continuing calibration standards. 12.15 Vortex samples mix for the standard 15 seconds. curve samples, matrix spike samples, and continuing calibration 12.16 Check to ensure 0.5 M TBA reagent isat pH 10. Ifnot, adjust accordingly. 12.17bTiocaerabocnhastaembpulfef,era.dd 1 mL 0.5 M TBA and 2 mLofthe 0.25 M sodium carbonate/sodium 12.18 Using an Oxford Dispenser, add 5 mL methyl-tert-butyl ether. 12.19 Cap each sampleand put on the shaker at a setting of300 rpm,for20 minutes. 1220 Centrifuge for 20 to 25 minutes at a settingof 3500 rpm, or until layers are well separated. 1221 Label a fresh 15mLcentrifuge tube with the same information as in 12.10. 12.22 Remove 4.0 mL of the organic layer to the fresh 15 mL centrifuge tube. 12:23 Put each hours. sample on the analytical nitrogen evaporator until dry, approximately 1 to 2 12.24 Add 1.0 mL to each centrifuge tube using agraduated pipette, 12.25 Vortex mix for 30 seconds. 1226FAitlttearchint2o.0a.21.u5mmnLylgolnasmseasuhtovfiilatlerotrolao3w-cvcosylruimnegeauatnovditarlanwshfeerntnheecessasmarpyl.e to this syringe. 1227mLaatbreixl,tfhienaalutsoovlivaelntw,ietxhtrtahcetsitounddyatneu,mabnedr,anaanliymsai(ls)nupmerbfeorramnindggtehnedeerx,trsaactmipolne. timepoint, 12.28 Cap and store extracts at room temperature or at approximatel4y C until analysis. 12:29oCroimnpclleutdee itnhesteuxdtyrabcitnidoenr,woarsksaphpereotp,riaatttea.ched to this document, and tape in study notebook ExtractionoEfTSP.F8O.S60from Liver 001P0ag2e 88of 14 13.0 DATA ANALYSIS AND CALCULATIONS 13.1 Calculations: 13.1.1 tCeanlcsuelpaatreattehe1.a0vemrLagealdieqnusotistoyfofhothmeogleivnearteh.omogenate by recording each mass of Average density (mg/mL) = Average mass (me)of the aliquots 1.0 mL aliquot 13.1.2 Cdailscpuelrasteed stohleiadmtoiussnute opefrlimvLero(fmgh)ompoerge1n.a0tmeLsuhsopmeonsgieonna)tues(ionrgctohnecefnotlrlaotwiionngof equation: gof Liver x Average density*of homogenate (gofLiver + g of Water) (mg/mL) * refer to 13.1.1fordetails. 13.1.3 Calculate standards aucstiunagl tchoenfceonltlroawtiinognesoqufatPiFonO:S and other fluorochemicals in calibration LofStmagndLairvderx/C|onmceLnthroamtoigoenn(autgem*L) =FionfaPlFCOoSncienntLriavteiron (g/g or mg/kg) *refer to 13.1.2 for details. 14.0 METHOD PERFORMANCE. 14.1 TspheecimfeicthMoDdLdeatencdtiloinmitliomiftq(uaMnDtiLt)atiisonan(aLlyOtQe)avnadlmuaetsr(irxefseprectiofiAct.taRcehfmeernttosMBDLanrdepCo)r.t for 14:2 tThheeqfuoalliltoywoifntghqeuaelxittryaccotnitornoalnsdamanpalleyssiasr.e extracted with each batchofsamples to evaluate. 14.2.1 Method blanks and matrix blanks. 14.2.2 pMraetcriisxiosnpoifktehaendexmtartarctiixons.pike duplicate samples to determine accuracy and 14.2.3oCfotnhteiniuniitnigalccaalliibrbartaitoinonvceurrivfei,cation samples to determine the continued accuracy 14.3 Refer to section 14 of ETS-8-7.0 for method performance criteria. 15.0 POLLUTION PREVENTION AND WASTE MANAGEMENT 15.1 ShiagmhplBeTwUasctoentiasindeirssp,osaenddiunsbeidoghlaazsasrdpicpoenttteaiwnaesrts,e fisladmimspaobsleedsionlvbernotkewnasgtleasiss dcoinstpaoisneedrsin located in the laboratory. ExtraEcoTftSPiF8oO.n6Sf0rom Liver 001029 Page9of 14 16.0 RECORDS 16.1 Complete or include tihnetehxetr3a-rcitnigonswtoudryksbhienedetra,tatsacahpepdrotporitahties.method, and tape in the study notebook 7.0 TABLES, DIAGRAMS, FLOWCHARTS, AND VALIDATION DATA 17.1 Attachment A, Extraction worksheet 17.2 Attachment B, MDL/LOQ values and`summary 17.3 Attachment C, Calibration standard calculation and concentrationworksheet 18.0 REFERENCES 18.1 The validation report associated with this method is ETS-8-6.0 & 7.0-V-1. 182 AMDT-EP-22, "Routine MaintenanceofUltra-Turrax T-25" 18.3 LFiAvCeTr-fMo-r1A.n1a,ly"sEixstrUascitnigonHoPfLPCF-EOlSectorroOstphrearyA/nMiaosnsicSpFelcutorroomcehtermyi"cal Surfactants from 1A 90 veecrepDocu0m 00e 0 nts 19.1 `EMTaSs-s8-S7p.e0c,tr"oAmneatlryys"isof Liver Extracts for Fluorochemicals using HPLC-Electrospray 20.0 REVISIONS R`eNvuimsbieorn, Reason For Revision ReDvaitsieon BimaETSR8.O6S0fomLivs (9 0) Page 100714 Study 7. Matrix Box#____ WK/Day_ Date Spiked/Analyst Surrogate S| approx. ppm| FC Mix Sd approx. 0.5 ppm| apFpCroMxi.x5Spipdm|| apFprCoxM.i5x0Spdpm| Comments actual # ppm| actual # ppm| acwal ppm | actual # # ppm s cov. r rr rr _-- wy rr rt _ ---- r rr rr r r rr rr r -- rt rrr rr r r t r rr rr r ----r ---- r rt r r rr rr r ee rm eeeee] rrr 1 rrr r r r r r r F T r rr e e T e[| --r 71 ee1 t eee] Ee toto ---- oriodnres TE Foe temper -------- | P_ otedt_ see _____--_ -- ---- __ ----_ ---- ----_ ---- ------ y Cont. Cal. Verifications usd the same matrix s for the standard carve Aachment B; MDLLOQ Values EximctionEoTfPSF:8O.S60fiom Liver 004.031 Page 110f16 MDL/LOQ values for rabbit liver `Compound PFOS MDL | LOQ| Linear Calibration Range (LCR) (PPb) | (Pb) | Approximate concentrations to be used for preparing the 845 Standard Calibration Curve 30 ppb - 1200 ppb PEPFWFOFOSOSSAAER-OF|13.50 0 |38 4115.1 [|165200 ppppppbbb--=--91102200000ppppbpp*bb PMRSOSSEA ||3 $3||3 215 6025 | 3[0Gp0ppbp-b=122000 ppppbb MDL/LOQ values in rat, bovine, and `monkey liver were not statistically determined. Two curves in eachofthese matrices were extracted and analyzed with the rabbit liver curves to determine equivalence. Responses in the rat, bovine, and monkey liver curves were equivalent to the rabbit responses, therefore, their MDL and LOQ will be assumed to be equivalent to those values as determined for the rabbit liver. * EtFOSE-OH estimates only for MDL and LOQ. Did not meet criteriafor validation. oue|oEE eeeteTeTy ore| pERx: Compound: PFOSA [oume|ewEE T[eEE ore ErI eS Compound: PFOSAA [uon|eEO|Be creTSTeSe H EEeE Liver range of average ave curve low std low std high std `high std `Extractionof PFOS from Liver 001032 Compound: EtFOSE-OH [ane|rEEm| So SemeeT SEE oGT Compound: PFOSEA | E GEE Eerm ee | THT EE EE ES | | S | E `Compound: M556 TTEe | ER Extractionof PFOS from Liver 001033 : Ton Pair Standard Curves -- Tissue Prep date(s): AnSaalmyptlee(sm)a:trix: TMaertgheodtiarneavliystieo)n:: FFCCmmiixxssttdd aappproxp.50r..0500op0 pp.p:m: SFuCrrmoigxatsetdstadpapprpor.ox50..0 10p0ppm:pm: Standard number: BF[ilEnaqanulkiplsmoielvvneetrn/tndueanmntbdieiTreN:r:: ASFcttFouOanSle|c|oncSPeuFndOctorSnaAeti||onPsoSFuflOcsSotAnacAn||darSEdusFlOicnoStnhEs|e|FFCSFumdOicSoxEncR|| S3c5o5n6e| Scone| Amiasnpiked | DeAnTy v0o5n0t0.[|0.u5g0m0l|| 0u5m0l ||0w5im0t||0s5p0n0 ||050w0b| | sp| ar | [C0300 0500[05m | 05w| 0500 |0500| ooorr 000 wi. e] | arr] [[0C5050000[0.0550000[05050000|[00550000|| 06550000 ||0o0sm0| 6.300 0500 |0500|0500|os00| | o0o0r20[]odteerr]] {aos |ote7| [s 5EX0e15m 00 [5s0o0o 5so0o0[3s0m0 s50e0e|[oo oo {{oodseer|] 0 ARC (6a S00 | 500|s00 | sno | se || oo |oier] CaFFlFicOulSla||tedPcFFooOnaSclAen||traFPtaFilOocSnosAnoAef||staEnCFdFaOalSrEd|[s iFnFFtOihnSaelEsAample conc | cone | ms | coe | come matrix Scone | SSiidrono| owe | rom | A[mATt spiked [F5ong9/rg fasngo/rgo[5 t|e5nge/9g |5e9nye9|a 50g/g | 1 ToTummsL | IW 32 i t BN I 599 55 599 5a 59 2 1 -- (iow[row | 119s[wis | es[mor|] Validated ranges-approximate concentrations [ Liver PFOS__ PFOSA|PFOSAA |ECFOSE-OH| _POAA | PFOSEA| [A oTv mien rbes --] ---- AtiachmensC:Standard Clclations ExEtToufsPsEseGSofcromoLinver Page 14014 001034