Document MG5mzXJjV4djDjxDJL4Xgkkwj

AR226-00 33 LEY ACUTE EASTERN OYSTER SHELL DEPOSITION TEST SUBSTANCE Identity: PFCe-r9f5l.uor(oo1c-tOancetsaulnfeosnautlef;moacnaiiydc,al1s,o1,2b.e2,r3e,f3e.r4r,e4d,5t,o5,a6s,6P,7F,O7,S8.o8r,8heptadecafluoro-, potassium salt, CAS # 2795-35-3) 3ReMmlaort knsu:mbTehre2t1e7st. sSuabsmtpalnecewaissastwohrietde upnodwedrera.mbSiaenmtplceonwdiatsiotnaskepnriofrrotom teelsetmiengn.taPluraintayldyesteesrmteicnhendiqtouebse. 90.49% by LC/MS, H-HMR, "*F-NMR and METHOD TMyeptheo:d:StaOtiPcPaTcuSte850.1025 GLP: Yes YSpeeacriceso:mpClreatsesdo:stSrteuadvyircgoimnpiclaeted 1999. Report completed 2000 SShueplplligerri:ndP.inCgu:mPmriionrstoOtyesstteirniCtioamtipoan,nrye,ceInntcl.,y Baltimore, deposited MD shell at the rwoausntdaekden(vteontrraelm)oevnedtwheasshrelelmroivmeduniufsoirnmglay tsomaplrlodelueccetraicsgmroiondtehr,. Care. rounded, blunt profile. Analytical Exposure monitoring: PFOS period: 96-hours measured at 0, 48, 96-hours Sttraetaitsmteinctalgmroeutphoadsst:heShpeelrlcegnrtowrtehduicnthiiobintiionnswhealsl gcralocwutlhatreedlaftoirveetaochmean vsihselulalgrinoswptehctiinotnheofnsehgealtligvreocwotnhtrionlh.ibTithieonEdCat5a0. value was The shell estimated by growth data was. eSvqaulauraetetdesftoranndorBmaarllilteylla'sndteshto,mroegsepencetiitveylyo.f variances Dunnett's using the test was Chiused to identify treatment reduction in shell groups growth which had a as compared statistically significant to the control, (<0.05) Test oyster age: unknown LPreentgrteha:tm3e3n.t8:(2N7.o8n4e1.5) mm, Test conditions: Dilution water: well water Natural seawater diluted to a salinity of 20%, with iDimlmuetdiioantwealtyerprcehceemdiisntgryth(edtuersitn)g: the 4-week period salinity: 21 (20-21) %, 000741 pH: 81(808.2) Toc <1.0mglL Stock and test dilution water at solution preparation: 9.1 mg/L and mixed for Primary stock prepared ~24 hours prior to use. in Awfittehrsmoimxiengw,hiptreimpaarryiisctuloactkesmoaltuetriioanlaspupsepaerneddceldetahrraonudghocuoltotrhleess stohleutfioounr.adIdtiwtaiosnaplrotpeosrttcioonncaelnltyrdaitliuotnesd.wiAtlhl dtielsuttisoonluwtaitoenrs taopppreeapraerde cnalteuarraalnsdeacwolaotrelre,sst.heDhuieghteostthceonrceleanttirvaeltyiolnowatStoaliunbailbiltey of PFOS with this in Cmaotnrciexnitsraaptpiroonxsimdaotseilnyg3.r3atmeg:/OLn.ce Exposure vessels: approximately 40L of 52L test polyethylene aquaria solution; water depth containing approximately 21 scmu.pplEeamcehntcahlaamlbgeare wdiaest ucsoinntginaunoueslleyctsrtiicrrpeadddtolecimricxuelra.te the sFpe,edCihnage:lAolcgearlocselslps,(TahnadlaIsssoicohsriyrsaispssepu.)downearneap,roSvkiedleedttoonema supplement naturally occurring growth rates during the test. algae and to maximize oyster Number of replicates: one NNuummbbeerr ooff ocoynscteenrtsrpaetriornesp:lifciavteep:ltuwseantnyegative control Water chemistry during the study: Dissol6v.e6d-o7x.y6gmegn/rLa(ncgoentr(o0l--e9x6pohsouurres)): 6.17.7 mg/L (3.0 mg/L exposure) PH range (0 -- 96 hours) 7.68.1 7.6-8.1 (control exposure) (3.0 mgL exposure) Test temperature range (0 - 96 hours) 222-22.3C (control exposure) 21.8-22.7C (3.0 mg/L exposure) Method of calculating arithmetic mean mean measured concentrations: RESULTS MNeoamisnuarledcocnocnecnetnrtartaitoinosn:s:Bk<LcoOnQtr,ol0,.316.,2,0.24.00,, 3.3, 13, 5.5, 1.9, 9.1 3.0 mg/L mg/L. Element value: 9966--hhoouurr ENCOsEo=C >=3.10.9mmgg/lLL(C.. nof calculable) All element values based on mean measured concentrations 000742 Statistical evaluationof shell growth: ECs values could not be calculated due to insufficient shell growth inhibition at the highest attainable concentration. Analytical Methodology: Analyses of test solutions were performed at WwiiltdhlimfaesIsntseprneacttiroonmaeltrLtidc. duestiencgtihoingh(pHePrLfCo/MrSm)a.nceWhliequniddcetherrommiantionggrtahpehy concentration of the test substance in the test solutions, the same and most prominent peak response for perfluorooctanesulfonate was used. No attempt was made to quantify on the basis of individual isomeric components. The LOQ (limit of quantitation) was 0.115. mg/L in this study. Samples collected at test initiation had measured values from 28 to 46% of nominal. Measured values for samples taken at 48-hours ranged from 15 to 41% of nominal. Measured values for samples taken at 96-hours ranged from <LOQ to 52% of nominal. `Summaryof analytical chemistry data: |mrmm[[emrara] Te[om [rpmecma| wos | woo| | Jmmeggae[[oomon[[e|n| [5[omsomommwo m|n]| [onLem [es [omsszm|sw mi|mwom| Biological observations after 96-hours: Oysters in the negative control and all PFOS treatment groups appeared normal and healthy throughout the exposure period. 000743 Shell deposition and shell growth inhibition at test termination: Fem&me] rorrme |] Te ee m oem| [a]] oCoemTenneLao] concwsons Mortality of controls: None CONCLUSIONS The potassium perfluorooctanesulfonate 96-hour ECs for the Eastern Oyster was determined to be > 3.0 mgL, the highest concentration tested and the practical limit of solubility in unfiltered seawater. The 96-hour no effect concentration was 1.9mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking = 1 meemences REFERENCES This study was conducted at Wildlife International Ltd., Easton, MD at the requestof the 3M Company. OTHER _ Last changed: 5/3/00 -------- 000744