Document MDyogeQ6D5opBqQ0OdMZVRvV
AR226 -- 0755
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity: A mixture containing perfiuorooctanesulfonate, which may also be
OrecftearnreesdutlofoansicPaFcOiSd), (FCCA-9S5#, 2o7r9a5s-3a9-c3o)m.ponent of FC-203CE. "-
Remarks: The 203CE (2/21/89).
3M production lot numberwas Current information indicates
519. itis a
The test sampl mixture of 1.84%
is
FC-
fPoFaOmSer,,330.%1%diseotdhiyluemneocgtlyylcsoullfbauttey,l 2e.t1he9r%,p6o0l.yo2x3y%etwhaytleern,e2m.o5n6o%ocStuylltpohneenyl
2et1h4erw,a0s.a1d2d%esdodaitaumnloamuirnylalsuclofnatcee,ntarnadti0o.no05f%2t0o0lylmtrgia.zole. Antifoamer SWS
The following summary applies to a mixture with incompletely rcehfalreacctttehreizteoxdiccoitnycoefntthraetfilounosorofchimepmuirciatliecso.mDpaotnae`nmtaoyfntohte atcecsutrsaatmepllye. METHOD
Method: OECD 209 YGeLaPr:CoNmopleted: 1990 tAenmapleyrtaitcuarle.monitoring: Dissolved oxygen concentrations, pH and Statistical methods: Results were determined by calculation of the % Tinehsitbiotirognainnirsesmpisroautriocne:ratAec.tivated sludge mixed liquor collected from the Metro Waste Treatment Plant, St. Paul, MN
Test condition:
SDiylnutthieotniwcasteewra:geMi:llippeorreOMEiCllDi-mQeTMtwhaotder rReeffeerreenncceesuabnsdttaenscet,so3l,u5-tdiiocnhlporreoppahernaotli,onw:asApsrteopcakrseodlubtyiodniosfsotlhveing w5a0t0erm,gthienn1b0rmoLug1hNt tNoatOhHe,podiinltutoefditnoci3pi0emntLpwrietchipMiitlaltiipoonrewiMtihlTiN-QHTM,SO4 saunbdsdtialnucteedwtaos1LmewaitshurMeildltio-QbTMew7a.t4e.r.TeTshtesupbHsotfatnhcee wreafseraedndceed vdiersescetllystaott2h0e0temsgt/Lv.esfsreolms.a sAtnatcikfosaomluetrioSnWpSre2p1a4rewdabsyaddilduetdingto2t0h0e0test amngtioffoSamWerSa2p1p4eairne5d00mimscLibMlieliin-wQaTMtewra.ter. The test substance and ~~ NTeusmtbveersosfelcso:ncNeonttrgiavteinons: three plus 1 reference substance and 2 Tbleamnpkercoantturroels: 20-22 C
001122
Tinoittiaall
psHus6p.e6nadneddfisnoallipdHs
and 6.9,
pInHitioalnTdSaSyoofft1e0s0t0inmgg:/L5.c0o3n3cegn/tLraatnidon
w2a2s132.g0L2,7 pg/HL,6.p8.H 6.6 while final TSS of 1000 mg/L concentration was
Element Basis: consumption.
Respiration inhibition as determined by oxygen
Remarks: Values corrected to 20 C for calculations.
RESULTS
Nanodmi1n0a0l0cmognicLentrations: 2 Blank controls, reference substance, 320, 560 Element values: 30-minute ECso = >1000 mg/L.
3-hour ECzo = >1000 mg/L. Element values based on nominal concentrations. R1e0m00armkgs/:L eTxhpeorseuwraescoancselnigthrtatriedouncwthioennincothmepaaremd otoouFfCfn-o2at0m3CfEorwmietdhoiuntthe
antifoamer.
TReesmtairngkswafiseldc.onTdhuectveadluoensarempioxrttuerde aapspldyestcortihbaetdmiinxtthureeTaenstdnSoutbtshteance fiuorochemical proportion alone.
CONCLUSIONS
Tdehteertemsitnseudbtsotabneo>s130-0h0oumrg/ELC.ao for activated sludge respiration inhibition was
Submitter: 3M Paul, Minnesota,
Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
DATA QUALITY
_
Reliability: Kiimisch ranking 2. Testing meets all criteria for quality testing. aHnoawleytviecra,l csoanmfpilremaptuirointyofwtahse naomtopurnotpeorflyflcuhoarroacchteemriiczaeldparnodpotrhteiosntiundythleacks
solution.
REFERENCES
----
TPhauils,stMudNy,
was Lab
RceoqnudeuscttendumbybetrheH314M80C-o3m,p1a9n9y0,.
Environmental
Laboratory,
St
001133
o0 mer0000000000
Last changed: 6/26/00
001134
-
Rl a0-=
bateziafo
.
ENVIRONMENTAL LABORATORY
rlyeigS
ACTIVATED SLUDGE MIXED LIQUOR
TypeTest Ackivaied Sludge Resgication nnibitionTe 0ECD%208
Source Meben Waste Teeabment Plat , StThaud Mu.
Date Obtatned___%/1q/d0
Date Used__/10 a
pa fal 9 Mixed Liquor Suspended Solids (MLSS) ~ SOX g/L Adjustment
g/L
Comments_
-
xr
Test Substance
-
Sample
Sample
DeshcirgihepsttioConbECUBCE
PHYA) Lolo, Cuallo
HG
(521/620/06w8)awlith
Adjustient
AniEhamat
SWS
Zid(xow"g8lL)
Comsente MLSSof loohngh,testadda.)initial2,0TgllsPinal(BHA,248g/L
Reference Substance
seme Descrtpcion 2 5 Diorspoenn
Sample PhAicLnest ot Fa a 5)A{ D eIunt
Comente Spork Solu pit "1
Exposure Conditions Test Duration 3 Hours
-- Comments Test Temperature 20-77
c
oT
_ eT -------------------- _---- fo00x1135
om0420 4.0Pw.o. ampleDescription
Environmental L--aWobrk Sheet
RESPOIREACTDIOANCTIINVHAITBEITDISOLNUDTGESET #209
Test
ats
.
Lab RequestNo.HI4BO-3
P08 --L of 3 Date: 3/19/90
Analyst: === =
sau laksaat 200.0501 dosage.SWS214 Stack.splubio: 20cnug/
Owls Mili Quister: Each lanl, = Ung
eau nisciole) Since git pM.
est Coornc. Bel
22ngS000aSmd0e0dn)--= gmerl1m0Sp00w0e0/watbeSrl00mSlmeedsnync.rSs0ew0ag0e41
al) StodSic OsoAlut.ion
mdlnomgiuclrmibpiakl m1 Totalalvolun
220
0+
Slo0
280 +
noa* S00 +
sComolTL 0 tr
259 + = +
e
259 + 254 14
259 + 25+ e
z+A0Y+ |,
+
200
+ 200
+ 200
+ 200
= SDO
= S00 = sha
= Sod
-_
OO
-_
---
SE e ,-_ e ---
-_--
.
OO
OOOO oo
000000 Oo
001136
:
-
Co
ENVIRONMENTAL LABORATORY
ndspa i}
#ege 20f5
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAK DATA
TeSosutrceSubosftaAncctei:vaFtCed-LSOlSudCgEe HMiLxeud Lhiq5u/o9r(:2A/l2e1t/r9n9)AvusittehATionvtiiaftaesreenrertSF\lLes,2S0e4oLualoaMlg,
DateReceived: 5//9/90
Date Used: _5//9/90
Analysts Eoprkrlle Bobideau_
30 - MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS mg
Concentration (mg/L) | 6.5 | 1.0 [1.5 2.0 2.5 |3.0 |3.
0
0
<
a 0
2
8
210
AL.
0
EH EE R 20
2 be 1
3 - HOUR EXPOSURE
ERan Concentration (ag/L) est Substance
conto 1
DISSOLVED OXYGEN CONCENTRATIONS mg
ME-MINUTE:
TEM
salselsz]ualusiui|aalz2lnal2dz0]
0 5.2.
42129
q
no a
ovo 1 sa|sslsJueluzlselzslzala[srlozle
o 5ofTenaosrestteudcorSludgeaSMixedtLeisqtueodr:: IInniittiiaall ((p5l,o0
FFiinnasl |9 , 8
001137
:
ENVIRONMENTAL LABORATORY ~~ ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #200
IR.
3
ate 3
Test Substance AL205CELabi 2CA/L2CU1L0A)TIwOiNtOhFARnEStULfToSatrir SWS218/(norms 5ast
30 - MINUTE EXPOSURE
ConcTeensttraStuibosntan(cneg/L)
CONTROL
220
0
000
Oxygen URptake Rate tRoCo2r0gected(1)
|ced | ca oupr
L[ o limre Ty pw aw,e
| le| o upn p
+ RIenshpifihbirieatintiioonniR nate (2) ------
CONTRO)
[oez |oz maao * |
Percent Difference in Controls() BCgo (95% Confidence Limits) mL
=.2% --
-
SelldR EROS
Test Substance
Concentration (mg/L)
Oxygen Uptake Rate
R
R Corrected!)
CONTRO)
|oso |om to 2p0C se|
0 a0
|08012 | ana] [osocn | popu |
ann |oss | diame |
+ Inhibition in
Respiration Rate
Q 2 f
CONTROL 1
[cm | oo me n 17
Percent Difference in Contro2,l20s7 )~ __~_
Ceo (95% Confidence Limits) mg/L.
:
r 1) Tew. Corrections --LL (ag 0,/L-nin.)
+
mT fsterTe=nce
10
&
Re, + Re
RC, + RC.
2) % Inhibition ra
x 100
Tshheoutlwdobecownittrohlirne1s5p% iroaftieoanchTaottehser
=hereO:xygen uptake rate (ng 2
1003 =Sample tempb.c I"
Or/L-nin.)
2
= 1.047, "x = T,0-bs 20C
= Control
001138
ENVIROMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
Dacemizz/an
pares
TypeTestAckivated RhudgeRespivabionlabibittodTest OECVE209
sovrcelpinWest eatwod Plast, SAPOMa
Dace Oveataed x20
Date Used___x 2% Ja
MPiixseidtLailqu0or fd ug Suspended Solids (MLSS) 1.85% g/L Adjustment
g/L
Com--e_mentsa . .
:
BEST COPY AVAILABLE
Test substance
Sample
Sample
DPHeisWncibrgihiaeplsthiocnomLcosern,rB,EoR Coal
AJtuuetgisftoasw)ieA(rdCseosot(4og/o1enils)
CommentsSpek sobaties opi la]
Silicones, (heratinn,
Adnan Ms
Reference Substance
Sasple Description \
highest
outesti, 10walL.
Sample PH iho) oR laal 1.0
? Adjustment
Coments_Sptous splution, 3H 2
`Exposure Condittons Tent Dwacton_2 Yours
Coments Test Temperature
-_ rere ---
|@- 2)
Sc
rere
--T
0
r--------r 00001x139
Sample Description
Sis 2k Arig STOCK SOITION:
Environmental La--b Work Sheet
RESPIOREATCIDOANCTTINVHAIBTIETDIOSNLUTDEGSET 209
Stock
and
Test Solutions 3 Si
Preparation a iors,
200000 /500wAL Beach
Lab RequestNoWI4BQ-S, F%0 --l-- of 5
Date: 8/22/90
po
Adcian = dng
--_--
EE
oO
ESEmAConc. pml rSitock0Blsoln npIeWrQ0wmat'er nfleeSdynp.orSSov0a0gme1 AiInomcuiicirmopberiasl00 mT1 efalnlvoline
1000
25 + isa +g + 200
= 500
--
---
-_
.
_--
_--
=
__-- OOOO _ -- -_-- _--------
:
001140
}
ENVIRONMENTAL LABORATORY
* ere 3
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #200
RAK DATA
TSeosutrcesuobfstaAncctei:vaSteId SSl2u1d4geAMnixteid aUauueorr:(MueltniW)aSsiltiecToneess(threposraPtiroant,,AS deiuagl , MMj,a
Date Received: 2/22/q0
Date Used: 5/2390
AnalysZpodele Zabides
30 - MINUTE EXPOSURE
concEeSTntraStuobsrtaoncoer) J .8110J1.5 2D0ISS[O2LV.E5D (OX3YG6EN31] C5ON8CETNTaRAoTIOlNSosgJo og un [0TH
hh] loonP oli lr ua lo.elasy ] 8
F SeH r ca
salsleasbla luocsaTliaTuoaBnZ de
3 - HOUR EXPOSURE
concaESnTiratSuibosntafnecgert)
DISSOLVED OXYGEWNE-CHOINNCUETNETRATIONS mg
1
iTE
conn 1 losliatJselua[usJanz2]2ulonus[1a Jia
i eCSmcERE: I TTTT
CONTROL IT
[se[wa [utlag]sslanai os] | 18
TPPHHestooffTehAmicpgtehirevasatttuerdceo:Snlc1ue8dngt-er1a9tMi0iox.nedteLsitqeudo:r: IInniittiiaall j(p2f1
FFiinnaall 0b.q8
e01324
.
+
ACTIVATED
ENVIRONMENTAL SLUDGE RESPIRATION
LABORATORY INHIBITION
TEST
#209
oo
IRHien5. Ane =/zn/og
TestSbstanceIS.204
AnkifoncCcAeLcCU(LA4TI1ONaO)FSRlESiULcTeS s
Cacpaottion,
age 3TH Advis VE
30 - MINUTE EXPOSURE
ConcTeensttraStubisotnanc(emg/L. CONTRO!
noo
Oxygen URptake Rate tRoCo2r0pCected(!)
|
|
owed
lous
1 ane
1 imap
% RIenshpiibriattiioonniRnate(2)
2
CONTROL
| eer | mer
FBeCrgcyen(t95%DiCfofnefriednecneceinLCinointts)rotstTMl)___y, gofy
Sl QUE RXEOSR
ConTceesnttraStuibosntan(cneg/L) Oxygen URptake Rate
RtoC2o0rrCected)
oT
oon
[asoa o
| lisse | gio
-
+ RIenshpiibriattiioonnRiante(2)
----
Fn wise a on CONTR
[wo 1 w aps
EPegrgcen(t95%DiCfofnefriednecneceinLCinitos)nteerot59s%) )__ :
_
(1) Temp. Correction= o* = (ng 0,/L-nin.)
(3) Differencez--L "dy 109
he, + RC.
RC, + Re)
hibition)
x * 00
Tshhoeultdwobecownittrhoilnre15s%piorafteiaocnh roattheesr
T,0=bOsxy=geSnamupplteatkeempr,atec (ng
O./L-nin,)
ol
61.047, "x = T,obs - 20C
C = Control
:
001142