Document M867gqDdJMpEzexqZY74kvXa

AR226-2929 FOR DU PONT USE ONLY HLR 411-85 HASKELL LABORATORY REPORT NO. 411-85 Title of Report STATIC ACUTE 48-HOUR EC501 OF TO Daphnia magna E. I. du Pont de Nemours & Company, Inc. Haskell Laboratory for Toxicology and Industrial Medicine P. 0. Box 50, Elkton Road Newark, Delaware 19714 Date Issued: S e p t e m b e r 13, 1985 'Company San'd-D eS,,,,(contain T S C i f W Title of Report STATIC ACUTE 48-HOUR EC50 TO Daphnia magna Summary _ _ exhibited slight acute toxicity to a warm freshwater macroinvertebrate Daphnia magna, under static unaerated test conditions during a 48-hour exposure period, The 48-hour EC50 was 210 mg/L, with a 95 confidence interval of 184 to 240 mg/L. Report by: Study Director: -- ^^ James W. Wetzel Technician S V fr M * Ralph G. Stahl Jr. Research Aquatic Toxicologist 2 |8S Mo/DayAr Approved by: U/Ji Carriof D. Litchfield Section Supervisor, Environmental Biology JWW:RGS:CDL:zh:5.11 Issued There are 7 pages in this report. Haskell Laboratory Report 411-85 HLR 411-85 Material Tested Composition: Haskell No. 15,866 Other Codes CAS Registry Number Sponsor: Polymer Products Department CHS 314 Material Submitted by: Polymer Products Department Washington Laboratory Parkersburg, WV Test Facility: E. I. du Pont de Nemours & Company Haskell Laboratory for Toxicology and Industrial Medicine P. 0. Box 50, Elkton Road Newark, DE 19714 Study Initiated/Completed: Initiated on 6/18/85 and completed on 7/4/85. Company Sanitized. Does not contain TSCA CRf SEC HLR 411-65 Introduction/Purpose: To determine the static acuta toxicity of freshwater macroinvertebrate (Daphnia roagnal (H-15,866) to a warm Materials/Methods (Environmental Biology Section Aquatic SQP-T04): Aliquots of water were collected from all Daphnia mass culture,tanks pooled, and filtered for use ae dilution water. The test material, was received as an aqueous slurry containing 56.55 of the active ingredient. All stock solutions and nomimal concentrations are based on the weight of the aqueous slurry and were not corrected for the amount of active ingredient. ^ I H i H i w a s prepared as a 50 mg/niL (density of slurry : 1.2; 1.2 g s 1 mL) stock solution in Milli-Q filtered distilled water adjusted to pH 8.5 with 1N NaOH and diluted with filtered laboratory-mass-cultore-well water to yield the desired nominal exposure concentrations. After mixing, 200 mL aliquots of each concentration were introduced into each of two separate 250 mL glass test vessels. Two identical vessels containing only filtered laboratory-mass-culture water were designated as HjO controls. Stability of the test and control materials in dilution water was not determined because it is not a critical factor in static acute aquatic tests. Ten daphnids less than 24 hours old from Haskell Laboratory-bred stock were placed into each test vessel. Haskell Laboratory-bred daphnids are identified by labeling the culture and test vessels. Food was not provided during the test. Test solutions were not aerated, and temperature was maintained between 20.1 and 20.2C. Photoperiod was maintained at 16 hours light:8 hours dark. Immobility counts and observations were made at 24 and . 48 hours after the exposure was initiated. Dissolved oxygen and pH were measured in the control and in the low, medium, and high exposure concentrations at the beginning and end of the exposure. The total alkalinity, hardness (EDTA), and conductivity of the H2 O control were measured at the beginning of the exposure. The 48-hour EC50 and 955 confidence limits were calculated by probit analysis (Finney, 1971). All records from this test and the final report will be maintained at Haskell Laboratory for Toxicology and Industrial Medicine or archived at the Du Pont Hall of Records. 'Company Sanitized i)c -4 HLR 411.8$ Results: Percent immobilities for Daphnia maqna exposed to H-15,866 are presented in Table I. The 48.hour EC50 was 210 mg/L, with a 95% confidence interval of 184 mg/L to 240 mg/L. The chemical and physical parameters measured during the test of H-15,866 are reported in Table II. Moat were within acceptable range8 for this study. By the end of the 48-hour exposure period, the D.O. fell to 30% of saturation in the high dose (500 mg/L). While this is below the level of D.O. recommended for this test (40%), it would not alter the conclusions drawn from this study. Discussion/Conclusion: __ has been tested for static acute toxicity with a warm freshwater macroinvertebrate. Daphnia magna^during a 48-hour exposure period. Under the conditions of this t e s t T ^ H ^ H ^ was found to exhibit slight acute toxicity. Footnotes: EC50 = That concentration which causes immobility to 50% of a test population during the specified time period. References: Finney, D. 3., (1971). Probit Analysis, 3rd. Ed., Cambridge University Press, 333 pp. -5- ompahy Sanitized. Does not contain TSCA C HLR 411-85 TABLE I RESULTS OF A 48-HOUR STATIC ACUTE TOXICITY TEST WITH Daphnia maqna EXPOSED TO 15,866. Nominal Test Concentrations (mq/L) 500 380 280 210 160 130 90 H2 O Control Observed Immobility () 24 Hours 48 Hours A* B* A* B* 40 30 100 100 40 30 90 80 20 10 90 50 10 0 60 30 00 40 10 00 30 20 00 0 10 00 00 * Replicate exposure chambers containing ten daphnids each. pdwpatty SaniUzsd. n' > 9 3 fiOT -6- 7SCACBI % HLR 411-85 TABLE II RESULTS OF PHYSICAL AND CHEMICAL PARAMETERS Physical/Chemical Parameters Dissolved Oxygen (mq/L) 0 Hr. 48 Hr. H 0 Hr. 48 Hr. Total Alkalinity (mq/L as CaCO-*)__ 0 Hr. EDTA Hardness (mq/L as CaCO,) . 0 Hr. Conductivity (unhos/cm) 0 Hr. ZGENominal Test Concentrations (mq/L 500 210 90 Control . (High) (Medium) (Low) 7.4 7.3 7.7 7.7 2.7 6.2 8.3 8.7 8.4 8.4 8.4 8.4 7.7 7.9 7.9 ' 8.0 mmm 101 90 180 J