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Biochemical Research Laboratory
T 3 .5 -6 -1 2
* The Dow Chemical Company
BLOOD BROMIDE LEVELS OP RABBITS RECEIVING REPEATED 7-HOUR DAILY EXPOSURES TO 25 PPM ETHYLENE DIBROMIDE
Signed
File
T3.5-6-12 ^
K No. *1715s9- *>'
Chg.
1219
Rept. By T. R. Torkelson
Checked
DISTRIBUTION
The Dow Chemical Co., Midland R. Hv Boundy CRI H.'*'H. McIntyre R. P. Perkins A. Hart N. Wright O. H. Hammer T. A. Hymas G. E. Lynn K. Barrons (2)
Midland Division
Dr. H. H. Gay W. L. Tisdale E. N. Luce L. M. Greene V. A. Stenger S. A. Shrader A. A. Asadorian
Texas Division
CRX Dr. D. J. Kllian
R. Langner R. W. Colby
Western Division CRI D. Elshere
JS. F. Kagy . . ,
PROBLEM
A maximum concentration of 25 ppm is considered acceptable for repeated
7-8 hour daily exposures of workmen handling ethylene dibromide. Using
laboratory animals, it was desired to know what blood bromide (inorganic)
concentrations are attained when repeated exposure to 25 ppm of ethylene
dibromide in air occurs. It is also important to know how fast the
blood concentration is established, how does it fluctuate when exposures
are missed, such as over weekend, and how rapidly does the level return
to normal when exposures cease.
DO 130598
CONCLUSIONS
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1) average maximum level of 14-16 milligrams of bromide per 100 ml. of blood (14-16 mg. %) was attained after 3 months of repeated 7-hour daily exposures 5 days per week. Blood serum levels would probably be
about 25 to 35# higher than this or 20-25 mg.
2) The blood concentration apparently had reached a maximum after 1 1/2 months of exposure.
3) Blood bromide levels before exposure (Monday morning) were about 2/3 to 1/2 the concentrations immediately after the 5th successive ex posure. (Friday afternoon).
4) The blood bromide levels were still significantly high 13 days after the last exposure.
THIS REPORT IS THE PROPERTY OF THE DOW CHEMICAL COMPANY
Biochemical Research Laboratory
T3-5-6-12 Page 2
PROCEDURE
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Pour healthy young rabbits about 3 1/2 months old were exposed 7 hours/ day, 5 days/week' for a total of 59 exposures during 93 days. The animals were exposed in a cubical 160 liter glass and Monel cjhamber to a calculated concentration of 25 ppm ethylehe dibromide, inj air. (21.<4-25.9 by analysis). Blood samples were taken by cardiac1 puncture before the first exposure so that each rabbit could serve as ,it's own control. Subsequent blood samples were taken as indicated in Figure 1 and Table I. Three ml. of blood were drawn for bromide determinations except on two occasions when larger volumes were drawn to allow deter mination of serum glutamic"bxalacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), Blood-Urea-Nitrogen (BUN), and extra blood bromide determinations. The routine bromide determinations
were made by E. T. Wagoner using Analytical Method MLS 5^*6, May 13, 1954. The transaminase, BUN and o'ftja.set of bromide determinations were
made by Ralph Langner and are reported in Table II. The findings from the transaminase and BUN determinations were not considered to be
significant.
With the exception of rabbit #968, all the rabbits lived, gained weight, and appeared to be normal during the entire experiment. Rabbit #968 died as the result of the third heart puncture and was replaced by rabbit #970. Except for the first two samples, rabbit #970 was assumed to have had the same number of exposures as the three original rabbits and the values obtained for this animal are average in with the rest.
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22 Ml i grama of Bromide Far 100 ml
20 Blood
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MAXIMUM, MINIMUM AND AVERAGE BLOCD BRCHEEE IEVELS CF 4 RABBITS RECEIVING
EEIEATED 7 HOUR BAIL! EXPOSURES TO 25 PPM CF ETHHENE DIERCHDE IN AIR T3.5-6-12
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Number of Exposures
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Biochemical Research Laboratory T3.5-6-12 Page 4
TABLE I
Summary of Inorganic Blood Bromide in Rabbits Exposed to Vapors of Ethylene Dibromide
Number Exposures
0 1 2 4
9 14
25 29 29 34 34 39 59 59
No. Days
0 1 2 4 11 18 34 38 4l
45 48 80 83 93
Blood Bromide mg. #
Rabbit Number
Day Time 9b5 966 9b7 22 nr Ave.
Mon. A.M. 0.52 0.39 0.53 Tues. A.M. 3.29 2.75 2.78 3.17 Wed. A.M. 5.00 4.83 4.91 Fri. A.M. 8.30 9-15 9 *77 Fri. A.M. 12.4 12.9 10.7 11.0 Fri. A.M. 13.3 12.6 10.8 13.0 Sun. P.M. 8.3 8.2 11.8 10.2 Fri. P.M. 14.1 13.7 14.5 16.8 Mon. A.M. 8.3 9*1 9.2 12.4 Fri. P.M. 16.1 15-8 14.8 18.0
0.53 0.49 3.00 3.00
4.91
9-07 11.7 12.4
9.6 14.8
9.8 16.2
Mon. A.M. 8.7 Fri. P.M. 20.9 Mon. A.M. 15.1 Thurs .P.M. 4.1
9.5 15.6 10.2
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10.6 8.7 5.6
10.4 18.1 12.7
3.2
9.5 16.3
11.7 4.0
TABLE II
Summary of Transaminase, BUN and Blood Bromide Determinations by Ralph Langner, Texas Division
25 Exposures in 34 Days
Rabbit
Serum
Number SGOT SGPT BUN Bromide
965 966 967 970
Jd
24
43
16.2
19.5
21
21
15.5
21.4
30
19
32.9
24.0
-
-
16.3
-
DO 130^01
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