Document M4nGL61pV9y8nB03joGkROkyM
TOXICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity:
Perfluorooctanoic PFOA ammonium
acid, salt,
ammonium Ammonium
salt; may also be referred perfluorooctanoate, PFO,
to as FC-
116, FC-126, FC-169, FC-143 or as the major component of FC-
1015. (Octanoic acid, pentadecafiuoro-, ammonium salt, CAS #
3825-26-1)
Remarks: The test sample is FC-1015. It's purity was not sufficiently characterized, though current information indicates is a 30% straight carbon chain version of FC-143 in 80% water. The 3M product lot number was *HOGE 205"
The following summary applies to the test sample as a mixture of the ctoesntcesnutbrsattainocnesIonfwiamtpeurristoielsu.tioDnatwaitmhaiyncnoomtpalcectuerlaytcehlayrraecltaetreitzoexdicity of cthoempteasrtesatomxpilcietywiotfhtthheabtroafncthheedt/essttrsauibgshttacnhcaei.n Daamtmaownaisuumsed to perfluorooctanoate homologue mixture in FC-143 vs. what is supposed to be the 100% straight carbon chain ammonium perfluorooctanoate in FC-1015,
METHOD:
Method: OECD 201, USEPA -- TSCA, Buideline 797.1050
Type (test type): Acute Static
GLP: No
Year study performed: 1996
Species: Source:
_ Selenastrumcapricomutum Originally obtained from the University
of
Texas
at
Austin
on
January 10, 1996, maintained in culture medium at T.R. Wilbury Laboratories,
Inc, Marblehead, Massachusetts.
Element basis: Algal cell counts (cells/ml), cell dry weights.
Exposure period: 96 hours
Statistical Methods: Probit analysis
Analytical monitoring: pH and temperature.
Test Conditions:
Algal Nutrient Medium: Sterile enriched nutrient medium per USEPA 1978 guideline. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was adjusted to 7.5 prior to use in assays.
Stock and test solution prepared by diluting 3.33
preparation: grams of test
A 3330 mg/L substance in 1
stock solution liter of water.
was Aliquots
004071
were then added directly to dilution water in test vessels to create test solutions.
Exposure vessels: 250 mi Erlenmeyer containing 100 mi test solution and capped with inverted glass beakers.
Agitation: Shaken continuously at 100 rpm.
Number of replicates: 3
Initial cell loading: 1.0 x 10
Number of concentrations:
cells/mL.
five plus
a
blank
control
Lighting: 400 foot-candles from continuous cook-white fluorescent lighting.
Water Chemistry:
pH range (0-96 hours):
7.5- 10.8 (control exposure)
7.4-7.6 (3,330 mg/L exposure)
Test temperature range (0-96 hours): 24.0-24.2 C (incubator)
RESULTS
Nominal concentrations: Blk control, 210, 430, 830, 1670, and 3330 mg/L
Algal Growth Response ECs, values
Exposure
Average Specific
(Contact)
Cell-Count
Growth Rate
Hours
mg/L (95% C.L
mg/L (95% C.L
[2a|" 2510 (1340->3330)
1700 (673->3300)
[ae1 >a | >3880 |
2040 (1190-3330)
[e6 | 1980 (1710-2360)
Element values are based on nominal concentrations.
Reversibility of Growth Inhibition: Aliquots of the 3330 mgL test solution `were diluted with algal medium and cultured for 72 hours. Based on growth observed in the recovery phase, the effect on algal growth was found to be algistatic
Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance.
CONCLUSIONS
FC-1015 exhibits a 96-hour ECs cell count value of 1980 (1710-2360 mg/L) and a 96-hour E Cso growth rate value of >3330 mg/L.
.
04072
The 96-hour No Observed Effect Concentration (NOEC) is 210 mg/L for cell count and 430 mg for growth rate. The Lowest Observed Effect Concentration (LOEC) is 430 mg for cell count and 830 mg. for growth
rate. This test substance was determined to be algistatic.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance
annodexapcltaunalatmieonasius rgeivmeenntass otof twhheyatmhoeu4n8t-hoofutresvtasluubesstwaenrcee iinn esoxlcuetsiosn.of Atlhseo,24
hour values. REFERENCES
Test was conducted by T.R. Wilbury Laboratories, Inc., of Marblehead,
Massachusetts at the request of the 3M Company, Lab Request number P1624, 1996.
OTHER Last changed: 5/24/00
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