Document M4mz5m0gJqEDp1kZ6Ozqab8LM
Submitted to:
BIOSEARCH, INC.
p.o.box 8598 philadelphiap,ennsylvania19101 t e Ie p h o n e ( 2 1 5 ) 848-4499 Project Number 77-1108A
3M Company 3M Center St. Paul, Minnesota 55101
Material: Sample Received: Date of Report: Test: Objectof Test: Procedure:
Results: Conclusion:
3M Company - T-1983CoC
11/2/77 Study Initiated:11/18/77Study Completed:12/15/77
1/5/78
Acute Oral Toxicity- Rats
To studythe acuteoral toxicityin rats of the subjectmaterial.
Four groups of 5 male & 5 female albino rats of the Sherman-Wistar Strain weighing between 200 and 300 gm were employed in this study. The rats were deprivedof food but not water for 24 hours prior to dosing. Each animal was weighed and dosed by direct administrationof the experimental material into the stomach by means of a syringe and dosing needle.
The sample was dosed as a 50% w/v suspension in water.
The following dosage levels were administered:
1.25 ml/kg. 2.50 mi/kg. 5.0 ml/kg. 10.0 ml/kg.
Followingadministrationthe animalswere allowed food and water ad libitum for the 14 day observation period during which time the rats were observed for signsof toxicityand mortalities.
See Table 1.
The subject material when studied in male and female albino rats has an acute oral LD50 of approximately 2.5 mi/kg of a 50% w/v suspension in water or 1.25 gm/kg. of the-originalsample.
Karl L. Gabrlel,V.M.D., P-h.D. Director
7
TABLE I Acute Oral Toxicity
Material: 3M Company - T-1983COC,as a 50%w/v suspension in water.
Dosage Level mi/kg 1.25
1.25
2.50 2.50
5.0 5.0 10.0 10.0
Number of Animals Dosed
5 males 5 females
5 males
5 females 5 males 5 females 5 males 5 females
Mortalities Days
1 2 3 4 5 6 7 8 9 10 11 12 13 14
0000 0000 0 0 0 0 0 0
Total Dead
14 Days
0
Total Survive
14 Days
5
0000 00 00 0 0 0 0 0 0
0
5
0 1 1 000000 0 0 0 0 0
2
3
02000000 1 0 0 0 0 0
3
2
5 -------- - - - - -
5
0
4 1 ------- - - - - -
5
0
5 -------- - - - - -
5
0
5 -------- - - - - -
5
0
The LD50 is approximately 2.5 mi/kg. of a 50%W/v suspension in
or 1.25 gm/kg. of the original sample.
At 1.25 ml/kg. (0.625 gm/kg.) the animals were slightly 14@thargicfoi then appeared normal. At 2.5 ml/kg. (1.25 gm/kg.) the animals were d 2 hours and did not regain normalcy throughout the observation period occurred as noted and the survivors were dehydrated and had lost weig
and 10.0 ml/kg. (2-5 gm/kg. & 5.0 gm/kg.) the animals were severely d 30 minutes and comatose within 2 hours.
Gross pathologic examination revealed nothing remarkable.