Document M4LXbQvd8x53mej9kNG280gEV
F3! STATS: TLJlvjrV-H:F^SI'T''V'
. --
: October 5, 1966
06PA4TMENT OF ZOOLOGY-------f
P.O. OXAWEJt Z
S'
state college. MississiPfi HtST^ '
PHONE (401) 3Z3-<321, EXT.acj. =-
Mr. L. C. ruhmeiscer Technical Services Superintendent. Monsanto Chemical Company Anniston, Alabama 36202 '
Dear Mr. Euhmeiscer:
The following is a brief report of our activities - in August.
srrA September, excluding the information contained in my letter
of September 19, 1966:
.
1) The sample of 551? provided by Monsanto was used' in
preliminary tests to determine its toxicity to bluegills
(levonis macrochlrus). Specimens measuring 1.5 to. 2.0
inches.in length were exposed in concentrations up to
1000 ppb at room temperature (72 i 2 F). All fish
survived. Pit? in relatively non-toxic compared with .
insecticides.
"
2) Previously, we reported tentative 36-nr TLffl values for bluegills to be about 25 ppb for methyl parathion and about 75- ppb for ethyl parathion. Additional tests have confirmed the .25 ppb value for methyl parathion but . indicate that Che parathion value may be' closer to 65 ppb. Although these points may be further refined, I am confident that they are accurate within t 2.5 ppb.'
3) '
'
-
On September 20, we collected 50 mosquitofish (Gambusia affinis) from Choccolocco Creek (4- miles east of Boiling Spring) and subjected them to bioassay. These screening" casts indicate 36-hr TL^ values of'about 80 ppb for parathion and' about 60 ppb for'methyl parathion. The . accuracy of these estimates will improve when additional specimens are.tested.
4) The following is a summary of water, mud, and,fish ssnples analyzed to dace:
DATE
COLLECTION SITE
SAMPLE
PESTICIDES (ppb)
S/13/66
Bridge - 4 miles E. Boiling Spring
Water
DDT .0.37 DDE .0.24 Methyl Parathion
trace 3 unidentified pecks
s
HARTOLDMONO014973
13/1*66
3ricge - 4 niles
DDT 294
E. Bolling Spring
Fish (Golden
DDE 172
Redhorse)
IDE 32
Interference in first S.5 minutes of effluent..
8/1S/1966 . Anniston Sewage Plant
Water
8/1S/1966
Bridge - Highway 0 21
Water
DDT 0.37
DDE 0.36
Lindane t 0.07
Methyl Parathion.0.44
3 unidentified peaks
DDT 0.62 ' DDE 0.63 '
TDE 0.15 Heptachlor trace 3 unidentified peaks
S/1S/I966
Jackson Shoal
' Water
DDT 0^20 DDE 0.31 Lindane trace 4 unidentified peaks
8/18/1966
Jackson Shoal
Hud
DDT and metabolites '
Interference for first 9. minutes of effluent and high background.
9/19/1966
4 miles E. Bailing Spring
t
Water (Main Stream)
DDT trade
DDE 0.69 TDE trace 4 unidentified peaks
S/19/1966
4 miles.E. Boiling
Spring
-
Water (Backwater).
DDT . 1.04 DDE 0.71 TDE 0.82 , 4 unidentified peaks
9/19/1966
Anniston Sewage Plant
Water
DDT 1.05
DDE 0.50 2 unidentified peaks
.P
.......
DSW 162356
HART OLDMONOOI4974
yszss&ssmm&z- a-fegaas.-,
.. v .
f
^ssaaas r-.
Page 3 - Mr. I.. C. "uhrmeiscer
DATE
COLLECTION' SITE
S/iS/i^oo
3 miles SW Coidwater * Spring
SAMPLE Water
.
PESTICIDES / nnVi'i
DDT 1.29 DDE 0.58 TDE 0.50 5 unidentified peaks
Thu water analyses are based on 700 ml samples. The August s.imoies were extracted with chloroform and the September samples with hexane, which may accounc for the higher levels of chlorinated hydrocarbons in the September samples. All samples were run on a Dow-11 column (non-polar). Several samples were run on a Q F-l column *.polar) for .-onf irs** Inn. About - or 5-unliier.tif icii peaks appear in nearly ell trie samples. The largest of these may be tsalachior. or nepcachlor epoxide, for which we do not presently have chromatographic standards. Also, samples from 4 miles East of . 3oiliitg Spring contain something that behaves similar to toxaphene. Enarln, aidrin, dielcrin and parachion were absent; nepcachlor, lindane, and methyl parat'nion were present in trace amounts only.
Sincerely yours,
Denzel E. FergusotCA Professor of Zoology bp
.y
DSW 162357
HART OLDMONOOI4975
IvCISSISSIPPI
tt_a:rs xji^/s^sirrv
Of.IoMJHDMuJNwTaO:f ZOOLOCy jTATicoiuoi, Mssaamsm,'
WONt (Ml) H3-3J, OCT. m*
19 January 1967
rlr. Z. C. Fuimeisoer
Technical Services Superintendent
Monsanto Chenlcal Company
*
Anniston., Alabama 3^202
Dear Mr. Fuhrmeister:
z'
^ ^tV
r*. H^r
QS to>'
VTe have been, bavins problems 1 High vater in Choccolocco Creek hindered attempts to replace fish in our cages until last veekand (jan. 15). Also, vater samples collected during high vater failed to provide useful information concerning microflora. These analyses Here done by our Microbiology Department and will be repeated when the stream flov returns to normal.
.
The sost vexing problem has been the appearance of a large peak on Gas chromatograms that coincides with parathion--this peak vas found in samples from all of oar stations. Initially, ve thought it to be parathion. However, when ve found it upstream from Anniston and because of its peculiar behavior on thin-layer places, ve decided that It vas not parathion. let, the .peak masked the actual parathion peak (if any) end prevented quantitation of parathion in samples. After rechecking vt equipment, reagents, and other materials used to process samples, ve finally discovered the source of the mysterious peak. It seems that hexane stored In polyethylene squeeze bottles picks up at least three contaminants, one of vblch has the exact same retention time as parathion. This has been a most exasperating
experience.
The following samples vere analysed vith the problem peak present, and ve quantitated the peak as thcogh it vere parathion.
DATS
LOCALUT
SAMPLE
INSECTICIDES
10/10/co
Bridge on Eivay 78 3 n_. Hast of Boiling
Springs
750 al vater
DDT - 0.4- ppb DDD - trace DDH - 0,4. ppb 3 unknown peaks
DSW 162366
CV96--J--0440--E DATE 04/02/01
PLFF EXHIBIT NO. 956
HARTOLDMONO014976
LCCAIZ2I
iO/iO/6o
Anniston Sevage Treatment Slant
SAMPLE 750 ml water
UJSSCTICIDES
DM - 0.2 ppb DDD - 0.2 ppb DD3 - 0.3 ppb Methyl Parathioa - 0.7 ppb 2 unlmovn peaks
lO/lO/co
Choccolocco Creek
cl. upstream free, month of Coldvater Creek.
750 cl water
DDT - 0.8 ppb DDD - trace DDE - 0.8 ppb BSC - trace
Methyl Parathioa -.1.1 ppb 3 unknown peaks
10/10/66 Bridge on Hlvay 109
rflnrgtl 1, _(l.01 g)
About 15 tall peaks, many of which interfere with pesticides. One
peek corresponds with methyl p&rethlon (13*550 ppm) and another with
nal&thlon (io.560 ppa.).. Many of these large peaks correspond exactly
uith peaks noted in mud samples collected downstreem fren Anniston and with those in water samples from Snow Creek at the Monsanto Plant.
We believe that a umber of materials that enter Snow Creek from the Monsanto Plant ere being accumulated by fish in Choccolocco Creek. Presumably these substances are non-toxic.
10/24/66
Choccolocco Creek mi. upstreem from.
mouth of Coldvater Creek.
Blnegill (3.29 g) (sterkvllle fish caged). -
At least 15 peaks and high levels of interference. Seme had retention times similar to parathioa, DDE, DDT, nslatMnn, and methyl parathioa.
12/10/66
Bridge on Hlvay 78 3 mi. East of Biollng Springs.
1000 ml water
-
BHC - trace
DDT - 2.3 ppb
DDE - 4.0 ppb
Malathiaa - 3-5 PP
Parathioxr* - 12-9 PF*1
.
his peak is the one mentioned earlier. If parathioa is present, it is masked by the contaminant.
DSW162387
HARTOLDMONO014977
ye. 1. C. jchrseister Rage 3
Haas
LOCAHXT
Z2/IO/00
Anniston Sewage Treatment Plant
* The contaminant peal.
SAMELZ 1000 ml water
INSECTICIDES
DDT - 2.0 ppb DDD - 0.3 ppb DDE - 1.8 ppb BBC - 0.2 ppb a HalAthlon. - 9.0 pp'0 Sarathion* - 26.8 ppb 2 unknown peaks
an water samples vere extracted vith chloroform. Tissue sample vere extracted vith Hexane/lsoprapenol (3:l)- AH samples vere' cleaned up on floxisil columns and evaporated to an appropriate concentration, for analysis. Analysis vas made on 10% DC-200 on Anahrcm ASS.
Ve have reason to believe that our values reported for USD may be ~ unreliable since ve encounter a fair amount of interference in this area.
The following data are derived free. tests of cholinesterase activity in 6 viTTg-m c cage one month In Choccolocco Creek at the Zisty 93 bridge. Originally tha fish vere collected from Country Club Lake near Starkville. Six control fish roa. Country Club Lake vere tested for comparison.
DATE
THEAEaHS
SHAHT TO. (mg) -
H ACh HEDRQLTZED 20` at 25 C
SSECX2TC ACTIVITY'
12/20/66 12/20/66 12/20/66 12/20/66 12/20/66 12/20/66 12/20/66 ^/2/66 12/20/66 12/20/66 12/20/66 12/20/65
Caged Caged Caged Caged Caged Caged Control Control Control
Control Control Control
34 36 37 40 44-
46
29 36 37 42
43 43
"
l.4o 1.4o
1.70 1.70 2.20 2.00 1.50 2.90
2.25 1.35 1.80 1.80
.
1.20 1.20 1.46 1.46
1.89
1.71 1.29 2.49
1-93 1.16 1.54 1.54
Ccrrr ol: T-jan - 1.66
Caged: Kean - 1.49
lmge^.1.20 - 1.89 ---------- Range - 1.16
- 2.49
J' DSW162368"
HART OLDMONO014978
Mr. 11. C. Tukrseister Page 4
Although the scans suggest a 10-2!$ inhibition in the caged fish, t_h_i_sdifference is not significant at the % level when tested with a z-zest. However, the t-value (2.501) barely missed being significant (t 2-571 with 5 d.f.), in spite of the inadequate sample size.
A preliminary literature search has revealed several reports of
enzyme inhibition by mercury, especially seme of the enzymes involved
in degradation of phosphate insecticides (See R. D- O'brien. i960.
Tonic phosphorous esters. Academic Press, E. T. p. 119). Ve have
sons studies underway regarding possible synergistic or additive
effects of mercury and paraehion. We are not yet ready to report our
findings.
In conclusion, it is obvious that all our data are incomplete.
A number of materials originating at the Monsanto Plant (via Snow
Creak) are shoving up in fish and mud samples taken downstream few
Anniston. We hope to mate a trip to Anniston soon and bring
chrcnatogrsms to illustrate this phenomenon. We have not been bl ^
to detect parathlon in Cnoccolocco Creak. Studies of passible
cholinesterase inhibition, in caged fish bold promise but are still
incomplete.
.*
Preliminary flow-through, studies and an investigation of tha . ' affects of mercury are Just now getting started.
Sincerely.
237 :vb
Denzel E- 7erj Professor of
DSW 162369 J*
HARTOLDMONO014979
0fAJ1MCNT Of ZOOLOGY
f. a. oiuwu z jutx cocuci. Mississippi sma
PHONC (Ml) 323*4331, (XT. 3(13
10 March 1967
1* C. Fur.;...alste.
Cechnical Services Superintendent
Monsanto Cr.e-~i.ai Company
'
i-umcscon, Alabama
Dear Mr. Fuhrmeister:
*<LiP 5 / 7ts/VL./r P
//// L*L> &7Z-.
Hie following is a brief report of some recent developments, especially
chose related to our trip last week.
*
First, some bad news. Both, of our gas chromatographs are down nd it
will be a while before we are able to have them, repaired. We have a large number cf samples being processed. .
On March 5, we collected a water sample from Dry Creek (at the Hiway
202 bridge). Ihe attendant at the service station said that fish had been
killed in the creek. Elis man claims that several cattle died as a result
of drinking from Dry Creek. He said that on Friday afternoons the creek
ns a real mess. However, when we collected the water samples, I observed
live scscuitofish in the creek, thus, I am not convinced that the stream `
is as bad as he thinks. Seme samples should be taken"on Fridays
used
for bioassay tests, of bluegilis.
I gave the Mississippi State Chemical Lab 2 bluegilis and a carp for mercury analyses. They seemed to doubt that they could do a quantitative analysis, but premised to do their best. If you people have competent, chemists who could do mercury analyses on some of the dead fish from down scream, I suggest that you get them busy, especially in view of the cholinesterase data below.
We caught a number of fish of several species at Jackson Shoals, including seme minnows and .small bass for residue analysis on GLC.
On March 5> we obtained 23 small bluegilis from Choccolocco Creek at
Jackson Shoals (ca. 150 yards below the confluence of Eastaboga Creek). .
Cholinesterase levels were determined for 20 of these fish using the brains
of 2 fish as a single sample. On March 7, cholinesterase levels were run
on 10 bluegilis from our control population at the Country Club. Procedure,
chemicals, and other variables were identical in tests of these, two groups
Cf fish.
:
DSW 162383
HARTOLDMONO014980
March 10, 15c7
Page 2
In Table 1, A-l through. A-10 are bluegills collected fron Choccolocca
Creek at Jackson Shoals j C-l through C-5 are control bluegiHs fron Country
03
Pable 1. Ccrpariscn of AChZ levels in Choccolocco Creek and State College
biuegills.
\
Sanple So.
Brain Wt.
Optical Density
ACh Hydrolyzed 20' 60'
Specific Activity
Average
A-l A-2 A-3 A-4
A-o A-7 A-w A-5 A-10
67.5 76.2 65 .1 91.2
81.7 56.6
1
5o.c
53.7 62.2 72.4
.209
2.07 ' 6.21
.210
2.07
6.21
.164
1.62
4.86'
.175
' 1-73 ' ' 5.19 '
.165 .233
. 1`83 ' 2.30
5.49 6.50
.
ro
CD
.242
7.14
265 .170
2.63 ' 1.67
7.89 5.01
.185
1.8l
5 A3
1.77 1.77 ' 1-39
1.48 1.58
-
1.98 2.04
2.25 1.43
'
1.55
1.72
Verf " o,.
101.4. `
.200
C-2 102.5
.167
C-3 50.8
.137
c-4 84.8
.170
C-5 110.9
.145
I.56 1.65 1.85 1.66 1.42
5.88 4.95 5.55 4.98 4.26.
1.68 '
1.41 1-59 IA3 1.22
. ..
1.47
DSW 162384
1
HARTOLDMON0014981
thrusister i'v7
Page 3
Please note that the Choccolocco Creeic bluegills have higher
cholinesterase values than aur controls. Although, these values may
r.zz jo absolutely correct, they are close enough to assure us that
there is no inhibition in the Choccolocco samples. Our average value
(1.72) compares favorably vith those of control bluegills from Choccolocco
Creek that were reported by the S3 Water Lab, especially if their very
large specimens are omitted. (Note: Sma.ll fish tend to have higher AChE
activity per unit weight of brain tissue.) Also, in our testis each value
represents era brains; they ran individual fish. Our controls compare
favorably with controls that we have caged upstream from Anniston in
Choccolocco Creek.
"
Table 2. Water quality data collected March 4, 1967.
Location
Time pE
Temp (C) .
D. 0.' (ppm)
" Surface Blbcv Depth
Choccolocco Creek 4 mi. 3. Boiling Spring
11:30 7-7
Anniston Sewage Plant
Noon 7-2
Chocccloccc Creek - Flat
Bridge
-
1:30 7-7
Choccolocco Creek - Hiway ' 2: CO 8.0 *93 Fridge
Choccolocco Creek - Hiway --- -- #77 Bricga
12.0
9-0
8,5
18.0 13.0
4.8 ' 8.0
----7.7 '
14.0
3.3
8.8
--- ,
3.9
3.5
We hope to get more ACh3 data soon. If fish continue to die in
Choccolocco Creek and if AChS levels continue to show no inhibition, it
vill be obvious that same, other lethal factor is operating. Such findings
should aid the lawyers in building their case.
'
I as leaving Sunday for a meeting in San Francisco, If you need to contact our group before the end of next week, please call Larry Ludke.
Sincerely yours.
"IF;va y
DSW 162385
\ipV^
Denzel 3.' Jergusos^X Professor of Zoolog^>S
HARTOLDMONO014982
f. 0. OtAWH Z
jut* coaiGi, Missasirn imz rHONt (MI) 223-<nl, (XT. MS
L. C. Fuhmeister
Technical Services Superintendent
Monsanco C'r.eni-al Company
'
Amis cor., Alabama
Dear Mr. Fuhmeister
N.
10 March 1967
_ _
'
?ts At L* A" ///; t*u ts7<L.
The following is a brief report of some recent developments, especially
chose related to our trip last week.
"
First, some bad news. Both of our gas chromatographs are down and it will be a while before we are able to have them repaired. We have a large number of samples being processed. .
0-r March 5, we collected a water sample from Dry Creek (at the Hivay 202 bridge). Ike attendant at the service station said that fish had been killed in she creek. This man claims that several cattle died as a result of drinking from Dry Creek. He said that on Friday afternoons the creek ns a real mess. However, when we collected the water samples, I observed live mcscuitofish in the creek, thus, I am not convinced that the stream ' is as bad as he thinks. Seme samples should be taken"on Fridays and used for bioassay tests.of bluegilis.
I gave the Mississippi State Chemical lab 2 bluegilis and a carp for mercury analyses. They seemed to doubt that they could do a quantitative analysis, but premised to do their best. If you people have competent, chemists who could do mercury analyses on seme-of the dead fish from down scream, I suggest that you get them busy, especially in view of the ekclinescerase data below.
Me caughz a number of fish of several species at Jackson Shoals, including seme minnows and small bans for residue analysis on GLC.
On March 5, we obtained 23 small bluegilis from Choccolocco Creek at Jackson Shoals (ca. 150 yards below the confluence of Easta'ooga Creek), Cholinesnorone levels were determined for 20 of these fish using the brains of 2 fish, ns a single sample. On March 7, cholinesterase levels were run on 10 bluegilis from our control population at the Country Club. Procedure, chemicals, and other variables were identical in tests of these.two groups
DSW 162383
HARTOLDMONO014983
March 10, l$c7
page 2
Ir* Table 1, A-i thracgh A-10 are bluegill a collected, front Choccolocco
Creel at Jackson Shoals; C-l through C-5 are control oluegills froa Country
Cauo i,aae
*
Table 1. Comparison of AChB levels in Choccolocco Creek and. State College
oluegills.
''
Sample Ho.
Brain Vt.
Optical Density
M 'A ACh Hydrolyzed 20' 60'
Specific Activity
Average
A-l A-2 A-3 A-4 '-"O A-6 A-7 A-6 A-5 A-10
67.5 76.2 '66.1 91.2 61.7 5c.o , 53.6 53.7 62.2 73.4
.209
2.07 ' 6.21
.210
2.07
6.21
.164
1.62
4.86'
175
'
.185 ~~~
1.73 ' 5.19 '
1-83
. 5.49
233 .242
' 2.30 2.38
6.90 7.14
.
.265
2.63
7.89
.170
;; ' 1.67
5.01
.185
1.81
5.43
1.77 1.77 ` 1.39
.
1.48 1.58 1.98 2.04
-
2.25 1.43
`
1.55
1.72
O< "J.
101,4 '
.200
C-2 102.5
.167
U "J 90.3
.167
c-4 ei.8
.170
C-5 no.9
.145
1.56 1.65 1.85 1.66 1.42
5.88 4.95 5-55 4.98 4.26.
1.68 1.4l 1-59 1.43 1.22
' . ..
1.47
DSW 162384
J'
HARTOLDMONO014984
-. c. w"--eister 10, i'll
Page 3
Please note that ilia Choccolocco Cresx bluegills have higher
cholinesterase values than our controls. Although, these values may
not jc absolutely correct, they are close enough to assure us that
chore is no inhibition, in the Choccolocco samples. Our average value
(1.72) compares favorably with those of control bluegills from Choccolocco
Creel that were reported by the S3 Water Lab, especially if their very
large specimens are omitted. (Note: Small fish tend to have higher ACh
activity per unit weight of brain tissue.) Also, in our tests each value
represents two brains; they ran individual fish. Our controls compare
favorably with controls that we have caged upstream from Anniston in
Choccolocco Creel.
. "
Table 2. Water quality data collected March 4, 1967.
Location
lime PE
2emp vC).
D. 0.' (ppm)
Surface Elbow Depth .
Choccolocco Creek 4 mi. 2. Boiling Spring
Anniston Sewage Plant
11:30 7.7 Noon 7.2
12.0 18.0
9.0 ' 8.5 `
4.8 '
' t
1
Chcccoloccc Creek - Flat
Bridge
-
1:30 7.7
13.0
8.0 - 7.7 `
Choccolocco Creek - Eiway ' 2:C0 8.0 #93 Bridge
Chcccoloccc Creek - Eiway --#77 Bricga
-- -
l4.0 ---.
3.3 ' 3.9
8.8 8.5
', '.
We hope to get more AChS data soon. If fish continue to die in
Choccolocco Creel and if AChS levels continue to show no inhibition. It
vill be obvious that some, other lethal factor is operating. Such findings
should aid the lawyers in building their case.
'
I am leering Sunday for a meeting in San Francisco. If you need to contact our group before the end of next week, please call Larry India.
IZ7:vb
DSW 162385
HARTOLDMONO014985
o- -L r-
C ~7 ;
6^RSITY
DEPA*TMN7 OF ZOOLOGY P.0. DJWWcX Z STATE COLLEGE, MISSISSIPPI ynn PHONE (401) 323-2I, EXT. 303
6 April 1967
~ C, Go
^
-Li. l> . .? mLZZZU2..3" ST orrical Services Superintendent lens er.ro Cr.e~.ical Cere any .-nr.rarer'.. Alan am
I -C *
A Z ...-
nj ^ D
D cr !^? '
>u; iO ` I^r
:-.r. yunrreister:
W\ $) r?~\ p S z'-'
The following retort includes'a simary of sore of out ^ ,
recent findings:
`
. , r.
b f ^26
vv ^
Table 1. Water-quality data collected March 22, 1967.
v.j j H'
'
.
location
lira
pH Temp'. (C)
D. 0. (ppa)
Surface
Elbow Depth
Chcccolccco
7:00 AM
C-- c.\ - 4 izi 2
--^ " > ^ ^**55"-
Chcccolocco Creek - Flat oracle
3:15 AM
7.U 7-2
Chcccclccco Creek - liway #53 Bridge
Choccolocco Creek - Hiway #77 Bridge
10:15 AM 11:00 AM
7-7 8.0
Anniston Sewage 7:30 AM 7.3 lrea.trerr Plant
Monsanto Ireatrent Facility
9:15 AM 6.2
Snow Creek
9:30 AM 11.2
et Trent Fence
13 8-9 . 8.9
13-5
8.0
7-8
14.0
8.5
8.4
15.0 9-0 . 8.8
19.0 17.0
None Detectable
6.5
*
--
22.0
8.0
---.
HARTOLDMONO014986
April 6, 1967
Page 2
Everything in Table 1 seems in order except the pH in the Trent ditch. Previous readings have always been very low or near 7.0.
You will recall my brief report of March 10 in which we
found normal ACh levels in bluegills collected at Jackson Shoals. On
March 23 we obtained.other specimens from Choccolocco Creek for
cholinesterase tests. All fish were kept alive until tested and each
fish was tested separately (we usually pool two brains to make a sample).
The samples in Table 2 were as follows:
'
Samples 1, 2 Distressed bluegills collected at Eureka Bridge. Samples 3> 4, 5, 6 Healthy bluegills collected at Jackson Shoals. Semples 7, 8, 9, 10 Healthy green sunfish collected at Jackson Shoals.
Table 2.
Sample Ho.
Brain Wt.
Optical Density
ACh Hydrolyzed 20' 60'
' Specific Activity
Average
108.1 mg
.130
2
35-3 Eg
093
3 100.7 mg
.133
b
bl.2 mg
.09b
5
33*2 mg
.111
6
26.5 mg
.145
7
35.4 mg
.145
3
28.2 mg
.107
9
3o.O mg
.142
10
26.7 mg
.lib
2.03 1.48 2.20 1.62 1.76 2.30 2.29 1.66 2.28 I.89
6.18 b.bb 6.60 b.86 5.28 6.90 6.87 b.98 6.88 5.57
"
1.76
1.27 1.88
'
1-39 1.51' 1.97 I.96 1.42 I.98 1.65
1.52 1.69 1.75
'r Or. March 25, Dr. L. L. Ellis and I collected 9 distressed bluegills and 3 distressed green sunfish at Eureka Bridge. These were
quick frozen on dry ice and kept frozen until analyzed on April 1.
DSW 162387
/
HARTOLDMONOO 14987
C. Ruhmeister
c, -So7
Page 3
These samples gave us peculiar results. Three of them failed to give a neanirgful reading and the others showed great variation. We don't know whether this cam oe attributed to technique, equipment, or the samples. ?or vhaz it is worth, the results axe shown in Table 3.
Table
.
; ample ICo.
Brslii Wt.
Optical Density
ACh Hydrolyzed 20' 601
Specific Activity
Average
1 77-8 mg 2 90.1 mg 3 100.0 mg ' 4 92.1 mg 5 82.3 mg 0s 72.8 mg
7 75.9 mg 3. 85.5~mg 9 83.7 mg 10 80.4 mg 11 80.0 mg
no data no data no data
.059 .Cjb .118 .093 .141 .074 .107 .120
O.76 O.98 1.44 1.21 1.79 0.61 1.38 1.54
2.28 2.94 4.32 3.63 . 5-37 1.83 4.14 4.62
.85 . .84 1.23 1.04';. 1.53 52 1.18. 1.32
1.06 1.00
This weekend we plan to collect additional specimens and freeze them. We will bring some of our control fish over and freeze them at the sane tine. This may indicate the source of our problems.
The following data refer to GLC analyses of samples collected
March 5, 1S67-
`
location
Samole
Results
ry Creek at ,,ivay 202 Bridge
700 ml water
2 small peaks - not pesticides or TCE.
Mono onto Treatment
700 ml water
5 large peaks unidentified
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Location
Chcccolocco Creak 4 mi. 2. Boiling Spring
Anniston Sewage Treatment Plant
S ancle 7C0 si water
700 si water
Results
2 peaks - one represents a trace of DDE.
3 peaks but * no pesticides
Choccoiocco Creek Kivay #S3 Bridge
700 ml water
' DDT - trace DDE - trace
(Note this water sample did not have the usual high interference that we have often attributed to Snow Creek.)
Choccoiocco Creek 4 si. E. Boiling Spring
Golden Redhorse**
DDE - trace DDT - 0.05 ppm
Choccoiocco Creek Plan Bridge
Bluegill (Caged)** (12.14 g)
14 major peaks
'The sample produced high background that interfered with detection of DI. DDT, DDD, heptachlor epoxide and methyl parathion. One peak corresponds to parathion, however, the compound causing the peak is less nolar than oarathion when tested in a partition-coefficient test.
Chocco1' occo Creek Jackson Shoals
Spotted 3ass ** (5-SO g)
17 major peaks Sane background as mentioned above.
* Two peaks resembled parathion and DDE but their partition coefficients (hexane - acetronitrile) proved otherwise.
** 'Zee C-LC tracings for these 3 fish are included as a part of this report. Ivcte the clean sample from the fish taken upstream. Many of the peaks in the downstream fish are those we have found in Snow Creek. Could rhis be the reason that the SE Lab did not do GLC analyses of fish tissues as a pant of their report? Note that we indicate retention .times for several pesticides on one tracing. The peaks do not match these times perfectly, indicating that they are not pesticides.
Sincerely yours,
j_- :vo
Denzel
sr\
Professor oi Zoologsu
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