Document M40znYNadx2RQ8VbnowqR06x9

A R U 6 _ 0854 MICROBICS MICROTOX TOXICITY TEST T E S T SUBSTANCE__________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates it is a mixture of 1.30% PFO S, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole. The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. M E T H O D :___________________________________________________________ Method: Microbics Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1996 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A 500 mg/L primary stock solution was prepared by dissolving 50 mg test substance in 100 ml_ Millipore Milli-QTM water. The pH of the stock solution was 6.3. An osmotic adjustment was made using NaCI dissolved in 10 ml_ stock solution. Appearance of test solutions was noted as "clear and colorless"." All test solutions were made by proportional dilutions.. Exposure vessels: Not given. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss 002288 RESULTS N om inal concentrations: Blank control, 31.25, 6 2 .5 ,1 2 5 , and 250 mg/L Element value: 5-minute EC50 = 217 (215 - 219) mg/L 15-minute EC50 = 146 (143 - 149) mg/L 30-minute EC50 = 124 (118 - 131) mg/L Element values based on nominal concentrations. R em arks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N CLUSIO N S_____________________________ ' ___________ ____________ The test substance 30-minute EC50 for Photobacterium phosphoreum was determined to be 124 mg/L with a 95% confidence interval of 118 to 131 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY______________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. R EFER ENC ES_______________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number P 2 5 8 1 ,1996. O TH ER ______________________________________________________________ Last changed: 6/26/00. 002289 05/26/2000 ENVIRONMENTAL LABORATORY STATUS REPORT 1 LAB REQUEST NO. P2581 REQUESTOR NAME: J MOHS-DAVIS PACE/SHARI LOUSHIN DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 08/14/1996 DESC: FC-603EF LIGHT WATER(TM) ATC 3%,AR-AFFF CONTRACT LAB(S): PACE ASCI EXP COMP DATE: 11/18/1996 DATE COMPLETED: 01/28/1997 PROJECT LEAD: SA BEACH PHONE NO: 651-778-7452 3M FAX NO: 651-778-6176 236-GL-04; 7-7490. Standard Testing. Formulation is on document #07 -7297-0. Results from Microtox Toxicity Test: Test Cone. mg/L 31 .25 62.5 125 250 Percent Light Loss 5-min Exp. 15-min Exp. 5.1 9.6 14 20 35 45 54 66 30-min Exp. 13 21 50 72 EC50 95% C.I. 220 (215 - 219) 146 (143 - 149) 124 (118 - 131) sab 10/31/96 Results from Activated Sludge Respiration Inhibition Test: Test Cone. mg/L 10 100 1000 1000 (dup) Percent Inhibition in Respiration Rate 30-min Exp 3-hr Exp. 00 00 00 00 EC50 > 1000 > 1000 sab 10/31/96 Results from Daphnia magna acute toxicity testing: Test Cone. mg/L 62.5 125 250 500 1000 Percent Immobilization 24-hr Exp. 48-hr Exp. 00 00 00 15 60 25 85 EC50 95% C.I. > 1000 - 480 (380 - 610) NOEC* 250 No Observed IEffect Concentration Results from toxicity test with Selenastrum capricornutum (green algae): Test Cone. Percent Inhibition in Average Specific Growth Rate 002290 mg/.L 24-hrs Exp. 48-hrs Exp. 72-hrs Exp. 96-hrs Exp. 05/26/2000 ENVIRONMENTAL LABORATORY STATUS REPORT LAB REQUEST NO. P2581 COMMENTS CONTINUED 31 .25 62.5 125 250 500 0 12 0 3 1 12 1 7 53 15 4 9 56 40 41 36 82 79 91 85 EC50 95% C.I. 170 (150 - 200) 290 (250 - 330) 275 (250 - 300) 290 (265 - 330) NOEC* No Observed Effect Concentration. 125 Effects were determined to be algistatic. sab 12/2/96 Results from Fathead Minnow acute toxicity test: Test chambers aerated with gentle, single-bubble aeration due to loss of dissolved oxygen observed in range-finder studies. Test Cone, mg/L 130 216 360 600 Percent Mortality 24-hrs Exp. 48-hrs Exp. 00 00 00 00 72-hrs Exp. 5 0 0 5 96-hrs Exp 20 5 10 15 LC50 > 1000 >1000 > 1000 > 1000 sab 1/27/97 SAMPLEi DATE CODE DESCRIPTION RESULT 1 FC-603EF AMBER LIQUID PACE ASCI ASCI ASCI P-CBOD-05 BOD-05 (CARBONACEOUS) P-CBOD-10 BOD-10 (CARBONACEOUS) P-CBOD-20 BOD-20 (CARBONACEOUS) P-CBOD-28 BOD-28 (CARBONACEOUS) COD *COD IC50-ALGAE *ALGAE ASSAY - IC50 BOD-REVIEW BOD TESTING REVIEW 48-HR-EC50 *DAPHNIA ACUTE TEST 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR MICROTOX MICROTOX - EC50 SLUDG-RESP SLUDGE RESPIRATION - EC50 216000 MG/KG 376000 MG/KG 446000 MG/KG 602000 MG/KG 740000 MG/KG 290 MG/L COMPLETE 480 MG/L >1000 MG/L 124 MG/L >1000 MG/L 2 DUP #1 MDL or (95% 2500 (265 - 330) (380 - 610) (118 - 131) (-) 002291 P-CBOD-05 BOD-05 (CARBONACEOUS) * = CONTRACT LAB DATA 110000 MG/KG 05/26/2000 ENVIRONMENTAL LABORATORY STATUS REPORT LAB REQUEST NO. P2581 SAMPLE DATE CODE CONTINUED DESCRIPTION RESULT 2 P-CBOD-10 P-CBOD-20 P-CBOD-28 DUP #1 ............ BOD-10 (CARBONACEOUS) BOD-20 (CARBONACEOUS) BOD-28 (CARBONACEOUS) 326000 MG/KG 439000 MG/KG 318000 MG/KG 3 MDL or (95% C.I.) BEST COPY AVAILABLE BEST COPY AVAILABLE 002294 best copy available BEST COPY AVAILABLE 002296