Document LpoYrMRQb9baR9r7drXg35XGz

ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as. PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC- 116, FC-126, FC-169, FC-143 oras the major component of FC- 1015. (Octanoic acid, pentadecafiuoro-, ammonium salt, CAS # 3825-26-1) Remarks: The test sample is FC-1015. It's purity was not sufficiently characterized, though current information indicates is a 30% straight carbon chain version of FC-143 in 80% water. The 3M product lot number was "HOGE 205". The following summary applies to the test sample as a mixture of the test substance concentrations In of water solution Impurities. Da with incompletely characterized ta may not accurately relate toxicity of the test samxpilceitywiotfhtthheabtroafntchheedt/essttrsauibgshttacnhcaei.n aDamtmaownaisuumsed to * compare to perfluorooctanoate homologue `mixture in FC-143 vs. what is supposed 10 be the 100% straight carbon chain ammonium perfluorooctanoate in FC-1015. METHOD: Method: USEPA -- TSCA, Guideline 797.1300 Type: Static acute GLP: No Year completed: 1996 Species: Daphnia magna Supplier: In-house cultures. Initial brood stock from Aquatic BioSystems, AFnoartlyCtoilclianls,moCnoiltoorraidnog: Temperature, pH, conductivity and DO Exposure period: 48-hours Test organism age: <24 hours old `Statistical method: Probit analysis Test conditions: Dilution water: Deionized water adjusted to a hardness of 160-180 mL (as CaCO) and passed through a particulate filer, ultraviolet sDtielriultiizoern,waantderaccthievmatiesdtrcya:rbon. Hardness: 176 mg/L as CaCOs Alkalinity: TOC: 108 mg/L as CaCO; <1 mg Lighting: Cool-white fluorescent lights at 130 foot-candles. A daily ppehroitoodpewraisodmoafin1t6aihnoeudrsthlirgohutgahnodut8thheoutresstdianrgkpewriitohd.a 15 minute transition 04054 Stock and test solution preparation: Test solutions were created by direct `weights addition. Exposure vessels: 300 mL glass beakers containing 250 mL test solution (9 cm depth). NNuummbbeerr Number ooff of dreapplhincaitdess:petrworeplicate: 10 concentrations: five plus a blank control Water Ccohnedmuicsttirvyidtuyrrianngget:he(0s-t4u8dyh:ours) 610 710 -- -- 620 720 pmhos/cm pmhos/cm ((c2o0n0t0roml gelxLpoesxuproes)ure)* pH range (0-48 hours): 88..12-88..32 ((c2o0n0tr0olmge/xLpoesxuproes)ure)* Temper2a0t.u4r-e2r0a.n7geC((0c-o4n8trhooluerxsp):osure) Dissol2ve0d.o3x2y0g8enrCa(n2g0e00(0m-g48/Lhoeuxrpso)s:ure)* 8.19.1 8.19.1 mg/L mg/L ((c2o0n0tr0olmge/xLpoesxuproes)ure)" * 2000 mg/L (second highest concentration) exposure values used because the highest concentration used resulted in total mortality by 48 hours. RESULTS Element values: `2448--hhoouurr EECCssyo == 11270900 ((7135050- -2020007)0)mmggl/LL 48-hour NOEC = 730 mg/L Element values based on nominal concentrations dReesmcarrikbsed: iTnetshteintgeswtassubcsotnadnucceterdemoanrkthsefimeilxd.tuTreheofvtahleuetsesrtespuobrtsetdanacpeplaysto that mixture and not the test substance. CONCLUSIONS The FC-1015 48-hour ECso was determined to be 1200 mg/L with a 95% confidence Interval of 730 to 2070 mg/L. The 48-hour No Observed Effect Concentration was 730 mg/L `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY 04095 Rteesltlianbgl.litHyo:weKviemri,scthhersatnukdiyngla=c2k.s Tihnifsorsmtautdiyonmoenetpsurtihtey ocfritthereiatefsotrsquuablsittaynce and actual measurements of the amount of test substance in solution. REFERENCES p---- TMeasstsawcahsusceotnldsucattetdhbeyreTq.uRe.stWiolfbtuhrey 3LaMboCroamtopraineys,,ILnca.b, oRfeqMaurebsltenhueamdb,er P1624, 1996. OTHER Last changed: 5/25/00 04096 BEST COPY AVAII ABLE ; Pray L ; FOE UL m , r tet 2Elan } Data Requirement: Guidelise Wumber C7 ua. enacrsea,cutdelise 197.1300 So 2 38 Lo k ay 15, 19% BE pin pews oy T C cdae alee y oa l a. pi RE TE ener go sent uid fan 5 geaa l p ane n la Ee a Se } | 0040978 at BESTCOPY AVAI ABLE 2. masorcomms A CA GRY She ER 4 5 maotctomeers seen of rite sd ries i wv et itte wee, ie 5 205 i fa Cia Ee ' signature Page osetia. tn uty ue fom eer nt i 5 wid Sa LoRh l EE e iLools HR rhS drae Sr al nL aanLe Bl RN = 002097 BESTCOPYAVAI ABLE wt rA e P PE ATr E ATAR, } TT SEs fi to rope Lr te ESE at aE pesT aE Ta EE sRepestle en emm omosiE in aetna Fi 17 survival and tmabaationf agin; Bupha 5 Fon Ji t; i o CRE " u4 l 02 33 0 i aR SPR Hh sg : oid SE CR 004099 : 75: Pe Ihe faT ecgeurltyomnieciyeRy eoif gFoEs-tI0LLotcaoElthoeieS deaytralrdL ss,laybmroaspphcSaarttreiplme,ianTiSso,khf1ee1sda t ; i 03umieTpnemeiidaStcoee1Foowsbober.t nREoovalresnmSanrtall s1e2sdtcTtiiotnoapaersd E E LcmmoencerTtheetstnesstaoAwnaSsdSApueriofTtoeremeI dsauniSdhA etruc4i"sotnatH aitcercSsounand.irFtT iecoencsceodwtintelhoasfteivde 2 2000 SeInosAenTcEuneeatSeicnIntoEfaFtEi-1ho13ynacoeenes(saosuvetaac,f2o430o,Fa1on0,0w1ib3n0ee6hs, A ES E tneT iLarveeT y-nAteE xpo4rsehowsswooro5fe5isSixnpnossLeurto s SthosetecstotnotnrwcoloindsaiTpcohiapoiondsss | oS1onnpScoeseTes CofedESDhaip6aindL sStToetFT R eee-tlohis=oCr1o9e0SCs55oItn3.iI0i an8.S4o8Aroe m.uatrTdoei=a4"n1ahi2Lee0easoke ET ST Riga JSR la ene a c a ial a is F PFrriN edn dG atasl hE Cn DaEE EaRR sO LE a Een ve 5 on REE ie LO A 0 A RTRs 004200 IAAERARI43N,OASS EERIE E orN Nor BESTCOPY AVAII ABLE b. 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Boyd| Fe R minrs oR iaeyrreae mse Sway a |ASaErra e[ | 27 T fcp(a MmSmU)ere opcon | r Tese soma o so her ma |e | STTo fn[erssasii CTR) vsa(Coemeerllt) ii er ----T [Se aSe ovpeetosmma Fero ariercd rtt coy sasertotxoanr DID Memos | ones |wFRneoeasheean eats vserastontcs-28 woorc0n) R[ava--na| saver a |eneiy d r"a "i 04119 PFOA and Associated Salts Environmental Fata and Transport echnical Report Summary- Assessment of the e|Britouaocrcouomcutlaantaivtee:ProSptearttoie(siosfh Atmesm)onium ner Date venta | sous ! tReocbhunsictasl rmepoart,, ||caruitsiuqyue by J. Giesy| Ecotonicity lomants MT oer See ey se aad ro SFT e ehwor[5| SDr azte Rasta copyofdata st o6-hour acute stati toxicity to fatnead mimow= FC-26__| aMeEnnv. Leab|| 11/1074 copyofdatasheet o J6-no oursaecute stam ti tity y tot bvego i sup nfishe FC-s 163_| |_s aMEm nv.n Lab || e4a670|c_|ocoppyooffddaataassnheeset I o6-nour acute stat toi to bluegi snfish- FC-143__| 9M Env. Lab | 572778_lcopyof datasheet ETwxoporespuorrte0s7:6".T0heoEnffHeacttcsohfabCiolnytionfuEogugssAaqnudeoGuroswth [|aonrdomseulravsiyv:alaonfdF"rSyuomfmFaartyheoafdHiMsionpnaotwho(iPoigmiecpahales June 1678 [Robust summary, E[Exxpaomsineadtitoon7s8o.f03Ffaotrh3ea0dDMaiysn.now (Pimiephales promelas) [EWGa&rGehBiaomn,omics,|| 1978 and Sept. [_croeppioersso.ftwo final 143 (Lot-37) LR. 5625 S 3M Env. Lab 5/8/80 _ roi to acivated siudge -FC-143 Exposure Recovery Algal Assay Test method (FC-149) 436-shuouirsacoutneesttyati toxicitoy Daptia magna -FC-143 om0_ 1S/716628,1 memLan | So6e2r, eee| [Chronic Toxicity of Fluorochemicals to Daphnia magna p|(rWoavtiedreFdlbeay)A-ScFlC-C1o4r3p., Daunldustthat,isMtiNcal analysis of data amen. Lav | 105 8/31/1984, 56 hour acute static tity 0 fathead mimow- Fx-1001 | 3M Ev. Lab [cRtooebcphuinssitcoafsl urdemaptmoaartry [sheets e[cRohbnuisctaslurmempoarrty, [[csohpeieetssof data [Robust summary, [Jestuemrmraerpyorrteport, | copyof data sheet. 04130 PFOA and Associated Salts t: Ecotoxity Elamonts : ihr bute Robust summary; 1[2ac8tivated side respiration nibion (OECD 208) FC- 407 c_|ospheyetosf data Robust Suma. icrotoxtest - FC-126 MEnv.Lab | 2087 csheoetosfpdayta cRoobpuyostf sduatmamary, 48-hour acute statitoxitcoDtayphnia magzna - FC-126 as07_|sRhoebesuss.tsummary, o6-nour acute state titytofathead minnow FC-126_| amen. Lab | 5107 [csheoeotsfpdayta Robust suman, 00s |Activated sludge respiration inhibition (OECD 209) - FX- MEnLab | 21690 [|[Rcsoohbpeiueetsssotsfudmamtaary. icrotox te-Fxs10t03 aMEn.Lab | 3790 c[oshpeieetss of data - Static Acuta Tricity of FX-1003 10th Fathead Minnow, | EHanmpvtoon,yNse,w fRioabtusretposrut,mmary. [Fimephatespromis ErHiaSmypsshiires,| 3/1510 [[pRroobtuosctolsummary.- mStaagtincaAcute Toxicty of FX-10to0th3e Daptvid, Daphnia | HHaammpptsonh,irNeow fpiraottorceoplort, Growth and Reproduction Toxicity Testwith N2803-3 and|LabTorRatoWriibesu,rync, neFreshwaterAla,Selenastrumcapricomutum Wardehead. WA| 1124/95 [nRaolbursetposrutmmary, [acu Toxity of N2603-3 t0 the Fathead Minnow, LabToRr.atWoirebsu,iyInc, Er -- Pimephals promelas MaTrRbiWihbetaiMdyA.| 124/96 [nai repor mcaugtneaToxicityof N2803-3 foteDap, Daphnia |LMaabrobralteohreiaeds,, MncA,| 3706_ i[nRaolbursetposrutmmary, [Robust summary, icrotoxtest C-143 iorotoxtest - rc-118 icrtoxtest - rc-1015:x MEm.Lab | 42am MEnv.Lab | 42406 MEmLa | sams R[csoohpbeiueetssstosfudmamtaary, c[shoeeptsoifdeatsa cRoopbiuesstosfudmamtaary. [sheets ) i c04121 |