Document LpoXE7MpaNqxmR1YZjOa2GONX

ACUTE TOXICITY TO FISH (RAINBOW TROUT) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component of FC-3052. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted.The testsample isFC-3052. Currentinformatioinndicatesitisa mixtureof5% PFOS, 39% diethyleneglycolbutylether,33.58% water,10% Sultonefoamer,6% sodium octylsulfate6,% sodium decylsulfate0,.36% sodium laurylsulfatea,nd 0.06% benzotdazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorachemical component of the testsample. METHOD: Method: Environment Canada Method EPS 1/RW9 July1990 Type: Acute static GLP: No Year completed: 1999 Species: Oncorhynchusmykiss Supplier:SpringCreek TroutHatchery,Lewistown,MT. Analyticalmonitoring:Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 96-hours Statisticamlethods: LC50 values calculatedby the Trimmed Spearman- Karbermethod, whilethe NOEC was determined viaFisher'sExact Test. Test fishage: Juveniles Length and weight: Length = 38 - 49 mm Average weight= 0.648g (wet) Loading: 0.242g/L Pretreatment: None Test conditions: Dilutionwater: DechlodnatedCityofDuluth,MN tap water,post- carbon. Water was aeratedfor24-hourspriortouse. Dilutionwater chemistry: Hardness: 44 mg/L as CaC03 Alkalinity: 44 -46 mg/L as CaG03 pH: 7.82 Lighting:Cool-whitefluorescentlights.A dailyphotoperiodof 16 hours light and 8 hours darkwas maintainedthroughoutthe testingperiod. Stock and testsolutionpreparation: The testmaterialwas added directly tothe dilutiownater on a weight/volumebasis. Concentrationdsosingrate:Once. StabiliotfythetestchemicalsolutionsN:otnoted. Exposurevessels:40 L glassaquaricaontaini2n6g.7L testsolution. Number ofreplicate2s: Number offishperreplicate1:0 Number ofconcentrationfsi:vpelusa blankcontrol Waterchemistryduringthestudy: pH range(0-96hours): 7.36- 7.82(contreoxlposure) 7.15 - 7.59 (125 rng/Lexposure)* Temperature range (0-96hours): 14.4- 15.4"C Conductivityrange (0-96hours): 126 - 136 pmhos/cm (controelxposure) 136 - 144 pmhos/cm (125 mg/L exposure)* Dissolved oxygen range (0-96hours): 5.8- 9.1mg/L (controelxposure) 4.4- 9.0mg/L (125 nig/Lexposure)* 125 mg/L (intermediatceoncentratione)xposure valuesused because the highestthreeconcentrationsused resultedintotal mortalitbyy 48 hours. RESULTS Nominal concentrations: Blank control6,2.5,125, 250, 500, and 1000 mg/L Element values: 96-hour LC50 = 177 (Clnot calculablem)g/L 96-hour NOEC = 125 mg/L Element valuesbased on nominal concentrations Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hourLC50 forrainbowtroutwas determined to be 177 mg/L. The 96-hourNo Observed EffectConcentration(NOEC) was determined tobe 125 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:Jimischranking= 2.Thisstudymeets the criterifaorquality testing.However, the studylacksi-nformationon purityofthe testsubstance and itlacksanalyticaclonfirmationofthe amount offluorochemicalproportion insolution. REFERENCES Thistestwas conducted by ASci Corporation,EnvironmentalTesting Divisiono,fDuluth,MN atthe requestofthe 3M Company, Environmental Laboratory,St.Paul,MN, Lab Request number W2039-2, 1999. OTHER Last changed: 6/26/00. ASci Corporation EnvironmentaTlestingDivision ASci StudyID# 5010-068 '4@ STUDY TITLE ACUTE TOXICITY OF FC-3052 TO RAINBOW TROUT (Oncorhynchusmykiss) DATASTANDARD Environment Canada Method EPS I/RM/9 July1990 STUDY COMPLETED July 19, 1999 TESTING FACILITY AScl Corporation EnvironmentalTestingDivision 4444 Airpark Boulevard Duluth,MN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATION NUMBERS AScl Study ID# 5010-068 3M Lab Request# W2039-2 Sponsor: 3M Sponsor Study ID# Nk'-1039-2 ASci Corporation Environmental Testing Division ASci Study ID# 501"68 1.0GENERAL INFORMATION StudyTitle Data Standard Sponsor Sponsor'sRepresentative Sponsor'sLab Request# TestingFacility PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitivTeest Dates Data Validationand Report Review Acute Toxicityof FC-3052 to Rainbow Trout (Oncorhynchusmykiss).-7 Environment Canada Method EPS I/RM/9 July 1990, 3M EnvironmentalTechnology and"Safety Services,Building2-3E-09, 935 Bush Avenue, St.Paul,MN 55133. Susan A. Beach, 3M Environmental Technology and SafetyServices,Building23E-09, 935 Bush Avenue, St.Paul,MN 55133. Tel No. (651)778-7452. W2039-2. AScl CorporationEnvironmental Testing Division,4444 AirparkBoulevard,Duluth, MN 55811; Tel.No. (218)722-4040. Joseph Dierkes. Joe Amato. Donald Mount. July15-19, 1999. Alan Mozol. Report Signature: Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport Ten yearsfrom the datestudyiscompleted. AScl CorporationEnvironmentalTesting Division,4444 AirparkBoulevard,Duluth, -I-MN I ; Tel.No. (218) 722-4040. Sponsor: 3M Sponsor Study ID# W2039-2 2 ASci Corporation EnvironmentaTlestingDivision ASci StudyID# 5010-068 2.0OBJECTIVE(S) To determintehe96-hLCIO,LC50,and 96-hNOEC (noobserveedffecctoncentratiofn)the testchemicalF,C-3052,forrainbowtrou(tOncorhynchumsykissu)nderstatitcestconditions. This studywas conducted accordingto Environment Canada ReferenceMethod EPS 1/RM/9 July 1990, "Acute LethalityTestUsing Rainbow Trout." Test Chemical Test Organisms DilutionWater (PC Water) 3.0 TEST METHODS Properties:FC-3052, dark amber/brown coloredliquid,specifigcravity ca. 1.1 (water = 1),vapor pressureca. 16 mm Hg calculateadt20'C, stable,and misciblewith water. Testconcentrations:0 (dilutiownatercontrol),62.5, 125, 250, 500 and 1000 mg/L based on range-findingtestresults.Test solutionspreparedby directadditionto each replicatteestchamber. Source: Healthy,juvenilerainbow trout(Oncorhynchus mykiss)obtained from a commercial supplier. Holdinglacclimation:Held and acclimatedfor24 days in the testdilution water.Less than 1% mortalityoccurredduring holding/acclimatiopneriod. At testterminationt,hey were 3.8-4.9cm in lengthand 0.414-0.928g in weight with a mean weightof 0.648 + 0.169 g. Loading ratewas 0.242 g/L. Use in test:Did not feed24 h beforetestinitiatioUns.ed 10 organisms/replicataend 2 replicates/tecsotncentration. Source: DechlorinatedCityof Duluth,MN, tapwater.The most recent chemical analysisisin Raw Data Package. CharacteristicsA:t testtime,the water had a hardnessof 44 mg/L (as CaC03) and a pH of 7.82. Aeratedformore than24 h priorto usingin the test. Sponsor: 3M Sponsor Study ID# W2039-2 3 ASci Corporation EnvironmentTaelstinDgivision ASciStudyID#5010-06g Exposure Chambers Incubation Observations Endpoint Calculations 7@pe: 40-L glassaquaria. Use in test: 26.7 L of dilutionwater or testsolution/chamber. During the test,keptcovered, except when experimentalobservationswere made. Solutionswere not aeratedduring the exposures. Duration: 96 h. Dailyphotoperiod: 16 h lightand 8 h dark,maintainedusingcool-white fluorescentlamps. Temperature: 15' I*C. Biological:Daily- Test organisms with abnormal behavior and signsof stress,and dead testorganisms. Water chemistry: (1)At testinitiationt-otalhardness and alkalinity(,2) daily-temperature,DO, and pH, and specificconductivityA.ll determinationswere made accordingto APHA methods (1998). 24-, 48-, 72-,and 96-h lethalconcentration(sLC50s),and 96-h LCIO and no observableeffectconcentration(NOEC). LCsos determinedvia Trimmed Spearman-Karber Method, LCIO determinedvia EPA lCp Method, and NOEC determinedviaFisher'sExact Test. 4.0 RESULTS Test Chemical Toxicity(Parenthetivcaluesare 95% confidenceintervals)P.ertinentdataare in Table 1. 24-h LC50: 330 mg/L (301 mg/L-362 mg/L). 48-h LC50: 177 mg/L (Not Reliable). 72-h LCSO: 177 mg/L (Not Reliable). 96-h LC50: 177 mg/L (Not Reliable). 96-h LCIO: 138 mg/L, Confidence Intervalnot Reliable. 96-h NOEC: 125 mg/L. Sponsor: 3M Sponsor Study ID# W2039-2 4 AScl Corporation EnvironmentaTlestingDivision ASci StudyID# 5010-068 Rangesof WaterChemistryParameters Totalhardness(asCaC03 mgll): 44. Alkalini(taysCaC03 mgll): 44-46. Temperatur(eOC): 14.4-15.4. DO (mglL):4.4-91.. pH: 7.15-7.82. Specificconductivi(tAymhoslcm):126-185. 5.0TEST QA/QC QA/QC Criteria Data Mortalityin controlexposure must not be more 0% mortality. than 10%. The lowestDO concentrationat test terminationmust be at least60% of theair saturationvalue at testtemperature. The lowest DO concentrationmeasured was 44% of theairsaturatiovnalueat 15.0'C. The lowest DO concentrationobserved was at the96 hour testintervalat the 125 mg/L NOEC. The lowestDO concentration observedat the time of complete mortalityin the250 mg/L testconcentrationwas 64% of saturation.The data show dissolvedoxygen deprivationdid not cause toxicitoybserved. Test durationmust be 96 h. The testdurationwas 96 h. ReferenceSubstanceToxicityTest (thistestis Data package for the sodium chloridetestwith optional). thisfishbatchisin Raw Data Package. Sponsor: 3M Sponsor Study ID# W2039-2 5 ASci Corporation EnvironmentaTlestingDivision ASci Study [D# Solo-068 6.0REFERENCES AmericanPubliHcealthAssociati(oAnPHA).1998.StandarMdethodsfortheExaminationf Water and Wastewater.APHA, Washington,D.C. Environment Canada. 1990.Acute LethalitTyestUsing Rainbow Trout.Conservationand ProtectionO.ttawa,Ontario.ReportEPS I/RM/9. Hamilton,M.A., R.C. Russo,and R.V. Thurston,1977. Trimmed Spearman-KarberMethod forEstimatingMedian LethalConcentrationisn ToxicityBioassays.Environ.Sci.Technol. 11(7):714-719;Correctio1n2 (4):417 (1978). U.S. EnvironmentalProtectioAngency. 1993.A LinearInterpolatioMnethod forSublethal Toxicity:The InhibitioCnoncentratio(nICp)Approach. West Inc.,& Universityof Wyoming, Laramie,Wyoming. 1996.ToxstatSoftwarePackage, Version3.5. Sponsor: 3M Sponsor Study ID# W2039-2 6 ASci Corporation EnvironmentTaelstinDgivision ASciStudy[D# 5010-068 TABLE 1. ACUTE TOXICITY OF FC-3052TO RAINBOW TROUT (0.mykiss) TestChemical Nominal Conc. (mg/L) 0 (control) 62.5 125 250 Soo 1000 Cumulative Number of Dead Organisms and PercentMortalitya - 24 h 48 h 72 h 96 h 0(0) 0(0) 0(0) 2(10) 0(0) 0(0) 0(0) 20(100) 0(0) 0(0) 0(0) 20(100) 0(d) 0(0) 0(0) 20 (1DO) 20(100) 20(100) 20(100) 20(100) 20(100) 20 (1291_ 20(100) 20(100) a Percentagesare in parenthesis. EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDWG 95% CONFI]DENCE INTERVALS LCs and NOEC 24-h LC50 48-h LC50 72-h LC50 96-h LC50 96-h LCIO 96-h NOEC Data (mg/L) 330 177 177 177 138 125 95% Confidence interval 301-362 Not reliable Not reliable Not reliable Not reliable Not applicable Sponsor: 3M Sponsor Study ID# W2039-2 7