Document LpmXYpL7G6ZmpJ7VEyk7yXMRz

AR 226 _ 0266 MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95,or as a component of FC-603F. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 501. The test sample is FC603F. Current information indicates it is a mixture of 0.5% PFOS, 7% diethylene glycol butyl ether, 82.95% water, 2% acrylic foamer, 4% Alkylether sulfate, 1.45% Xanthan gum, 1.4% calcium acetate, 0.07% N-(3Chloroallyl)hexaminium chloride, 0.5% triethanolamine, 0.05% sodium hydroxide, 0.03% cis-1-(3-Chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect the toxicity of the fluorochemical componentofthe test sample. METHOD: Method: Microbics Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1994 Species: Photobacterium phosphoreum `Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression pTersotgroarmgaprnoivsimdesdofuorrcMei:crMoitcorxobics' Reagent, Lot AM025 Test conditions: Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A 2000 mg/L primary stock solution wasprepared by dissolving 200 mg test substance in 100 mL Millipore Milli-QTM water. The pHofthe stock solution was 6.7. An osmotic adjustment was made using 200 mg NaCl dissolved in 10 mL. stock solution. Appearanceoftest solutions was noted as "clear and colorless". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Number of replicates: two `Number of concentrations: Four plus a blank control Element basis: Percent light loss 002463 RESULTS Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L Elementvalue: ~~ 5-minute ECso = 480 (450 -- 510) mg/L 15-minute ECso = 390 (3--74200) mg/L. 30-minute ECso = 340 (320 -- 350) mg/L. Element values based on nominal concentrations. Remarks: `Substance Testing was conducted on Remarks field. The values a mixture reported as described in the Test applytothat mixture and not the fluorochemical proportion alone. CONCLUSIONS _ The FC-603F 30-minute ECso for Photobacterium phosphoreum was determined to be 340 mg/L. with a 95% confidence interval of 320 to 350 moll. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY Rteelsitaibnigl.itHy:oweKvieirm,isscahmrpalnekipnugri=ty2.wTahsisnosttupdryopmeerleytschtahreacctrietreirzieafdoarnqudatlhietystudy lacks analytical confirmationofthe amountoffluorochemical proportion in the solution. R~ EFERENCES ~~ 000 Thisstudywas conducted by the 3M Company, Environmental St. Paul, MN, Lab Request number M1655, 1994. Laboratory, PER -- Last changed: 6/26/00. ---- ------ 002464 IMENVIRONMENTALLABORATORY Pag\e of 1 Microtox Test Data Summary BEST COPY AVAILABLE LR:M1655 Date: 5/20/94 rater 2 SAMPLE: FC-603F (Lot #501) SarpaltosCon|s ma%TReduI16nBcminhtmi|niasoca9nncemn 2i2s0 3s7 53 3s 520 2 5 5 eee fO00__J... 88 yrolnnsifBens atens rl nme. EC50 480 mg/L| 390 moi| 340 mgiL. 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