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A tia & - 480 DuPont-11603 TRADE SECRET Study Title H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss Test G u id e l in es: U.S. EPA OPPTS, 850.1400 (199b) U.S. EPA TSCA EG-11 (1982) OECD TG 210 (1992) A u th o r : Robert A. Hoke, Ph.D. Study Com pleted o n : September 5, 2003 Performing Laboratory: E.I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 L aboratory P r o ject ID: DuPont-11603 W ork Request Number: Service Code Num ber:j Page 1 o f 137 H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT This study was conducted in compliance with U.S. EPA TSCA (40 CFR part 792) Good Laboratory Practice Standards, which are consistent with the OECD Principles o f Good Laboratory Practice (as revised in 1997) published in ENV/MC/CHEM(98)17 and MAFF Japan Good Laboratory Practice Standards (59 NohSan Number 3850) except for the item documented below. The item listed does not impact the validity o f the study. Test substance characterization and stability analyses were performed at Regional Analytical Service (RAS), Deepwater, New Jersey. None of the aforementioned analyses were performed under Good Laboratory Practice Standards; however, the analyses were conducted in compliance with IS09002 regulations. All o f the analyses are considered sufficient for the purposes o f this study. Applicant / Sponsor: E.I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. Study Director: ___________ _k__ / - Robert A. Hoke, Ph.D. Senior Research Ecotoxicologist and Manager S ' S ip A Date Applicant / Sponsor: DuPont Representative ianitlzd- Does -2 - Date mlSOACBl H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 QUALITY ASSURANCE STATEMENT Haskell Sample Number(s): 24678 Dates o f Inspections: Protocol: October 29, 2002 Conduct: February 14,21, 2003; March 5,6, 2003; May 15, 2003 Records, Reports: August 11-15, 2003 Dates Findings Reported to: Reported by: Quality Assurance Auditor Company San't'^ed. Does not coH a'n TSCA CBl H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 CERTIFICATION We, the undersigned, declare that this report provides an accurate evaluation of data obtained from this study. Analytical Evaluation by: k _S Bogdan Szostek, Ph.D. Senior Research Chemist Date Statistical Analyses by: /y J u liiC. Reynollddss,, M.S. Staff Biostatistician S " X )03 Date Issued by Study Director: f l iu /' /( j Robert A. Hoke, Ph.D. Senior Research Ecotoxicologist and Manager >5" J y ? / - >Pqc0 Date Compaq Sanitized. Does -4 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE OF CONTENTS Page G O O D L A B O R A T O R Y PR A C T IC E C O M P L IA N C E S T A T E M E N T .........................................2 Q U A L IT Y A SSU R A N C E S T A T E M E N T ........................................... 3 C E R T IF IC A T IO N ........................................................................................ 4 L IST OF T A B L E S ........................................................................................ 6 L IST OF F IG U R E S ..................................................................................... 8 ST U D Y IN F O R M A T IO N .......................................................................... 9 ST U D Y P E R SO N N E L ................................................................................. 10 S U M M A R Y .................................................................................................... 11 IN T R O D U C T IO N ......................................................................................... 13 M A T E R IA L S A N D M E T H O D S ............................................................. 13 A. Test Guidelines......................................................................................................................... 13 B. Test Substance...........................................................................................................................13 C . Dilution W ater........................................................................... 14 D. Test Organism Culture............................................................... 14 E. Test Methods..............................................................................................................................15 F. Sample Preparation and Chemical Analysis...........................................................................16 G. Statistical Analysis.................................................................................................................... 19 R E S U L T S ........................................................................................................ 20 A. Analytical Report......................................................................................................................20 B. In-Life Report............................................................................................................................ 21' C. Statistical Analysis Report....................................................................................................... 22 D IS C U S S IO N ................................................................................................. 23 C O N C L U S IO N ............................................................................................. 23 R E C O R D S A N D SA M PL E S T O R A G E .............................................. 24 R E F E R E N C E S.............................................................................................. 25 T A B L E S ............................................................................................................................................................... 26 F IG U R E S ........................................................................................................... 126 S: -5 - /N H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 LIST OF TABLES Page 1. CHEMICAL CHARACTERISTICS OF HASKELL LABORATORY WELL WATER.....................................27 2. MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS......................................................... 28 3. WATER CHEMISTRY OF THE DILUTION WATER CONTROL, IPA CONTROL, AND HIGHEST TEST CONCENTRATION WITH SURVIVING ORGANISMS..........................................................................38 4. TEMPERATURE (C) OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST..........................................................................40 5. pH OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST............................................................................................................................... 41 6. DISSOLVED OXYGEN CONCENTRATION (mg/L) OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST....................................42 7. SUMMARY OF HATCHING, SURVIVAL, ABNORMALITIES, AND SWIM-UP FROM HATCHING TO THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST................................................................................................................ 43 8. SUMMARY OF SURVIVAL, ABNORMALITIES, AND GROWTH FROM THINNING TO TEST END FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST.......................................................................................................................................................46 9. DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST....................48 10. IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST......................................................... 54 11. 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST.........................................................60 12. 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST.........................................................66' 13. 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST..........................................................72 14. 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST................................................................. .......78 15. 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST....................... ................................. 84 16. DILUTION WATER CONTROL: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST....................................................................... 90 17. IPA CONTROL: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST................................................................................................................ 91 18. 0.63 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST................................................................................................................ 92 19. 1.3 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST..................................................... 93 not contain TSCA CBI flrtmpany Sanitized. D oss -6- /*"% ' H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss______ __________________________________________________ DuPont-11603 2Q 2.5 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST................................................................................................................94 2 1 . 5 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST............................................................................................................................... 95 22. 10 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST............................................................................................................................... 96 23. WATER CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST......................................................... 97 24. IPA CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST.......................................................100 25. 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST.......................................................................103 26. 1.3 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST........................................................................ 106 27. 2.5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST........................................................................ 109 28. 5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST.......................................................................112 29. 10 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST.......................................................................115 30. DILUTION WATER CONTROL: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST......................................................................................................................................................................... 118 31. IPA CONTROL: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST..................................119 32. 0.63 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST..................................120 33. 1.3 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST..................................121. 34. 2.5 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST.................................122 35. 5 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST..................................123 36. 10 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST..................................124 37. SUMMARY TABLE OF STATISTICAL ENDPOINTS..................................................................................... 125 Company Sanitized. Does not contain TSCA CBI -7 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 LIST OF FIGURES Page 1. PHOTOGRAPH OF AN EMBRYO AT APPROXIMATELY 22-HOURS POST-FERTILIZATION........... 127 2. REPRESENTATIVE ANALYTICAL CALIBRATION STANDARD CURVE FOR H-24678: ^ .......... 1 2 8 3. REPRESENTATIVE HPLC CHROMATOGRAM OF A CALIBRATION STANDARD SOLUTION..........130 4. REPRESENTATIVE HPLC CHROMATOGRAM OF A DILUTION WATER CONTROL SOLUTION.............................................................................................................................................................. 132 5. REPRESENTATIVE HPLC CHROMATOGRAM OF AN IPA CONTROL SOLUTION..............................134 6. REPRESENTATIVE HPLC CHROMATOGRAM OF A H-24678 TEST SOLUTION...................................136 Company Sanitized, Does not contain TSG GBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus myUss STUDY INFORMATION Substance Tested: 1 Svnonvms/Codes : Haskell Number: 24678 DuPont-11603 conditions o f the study; no evidence o f instability was observed. Sponsor: E.I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. Study Initiated/Completed: October 29, 2002 / (see report cover page) Company Sanitized. Does not contain TSCA CBl -9 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss STUDY PERSONNEL Study Director: Robert A. Hoke, Ph.D. Primary Technician: Jeffrey T. Turner, Sr. Animal Culturist: Barbra D. Ferrell, B.S. Management: Jeanette M. Erhardt, Ph.D. Analytical Associate: Keith B. Prickett, B.S. Analytical Chemist: Bogdan Szostek, Ph.D. Management: S. Mark Kennedy, Ph.D. Statistical Analysis: Julia L. Reynolds, M.S. Management: John W. Green, Ph.D., Ph.D. Ecotoxicology Report Preparation: Wanda F. Dinbokowitz Management: Nancy S. Selzer, M.S. DuPont-11603 Company Sanitized. Does not contain TSCA CBi -10- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 SU M M A RY GUIDELINES: The effect o f H-24678 (purity 100%) on hatching, growth and survival of rainbow trout, Oncorhynchus mykiss, embryos, alevins, and fingerlings was assessed in a flow-through, 90-day early life-stage toxicity test in accordance with the appropriate Good Laboratory Practice standards and test guidelines (U.S. EPA TSCA EG-11; U.S. EPA OPPTS 850.1400, OECD 210). NOMINAL TEST CONCENTRATIONS: 0.63, 1.3, 2.5, 5, and 10 mg/L H-24678, dilution water control, isopropyl alcohol (IPA) control MEASURED TEST CONCENTRATIONS: 0.75,1.3, 2.8, 5.6, and 10 mg/L H-24678 w ith p B J |||^ p o n e detected in dilution water and IPA controls (LOD, 0.0022 mg/L) 0.54,1.0, 2.1,4.5, and 10 mg/L H-24678 w i t l J p H H ^ n o n e detected in dilution water and IPA controls (LOD, 0.059 mg/L) 0.63, 1.3, 2.7, 5.0, and 11 mg/L H-24678 w i t l p | B H H H B | none detected in dilution water and IPA controls (LOD, 0.042 mg/L) AGE OF TEST ORGANISMS: Approximately 22.5 hours post-fertilization at test start SOURCE OF TEST ORGANISMS: Unfertilized eggs and sperm (collected from 3 female and 1 male brood fish) were obtained from Troutlodge Inc., (Sumner, Washington) TEST DESIGN: A total o f 80 embryos were exposed per concentration (20 embryos per embryo cup, 2 cups per replicate, 2 replicates per concentration) at test start. On day 45, after swim-up had begun in the dilution water control, the fingerlings were thinned to a total o f 30 fish per concentration (15 fish per replicate, 2 replicates per concentration). Analytical verification o f H-24678 concentrations was made on test solutions sampled on day -1 , day 0, once weekly, and on day 90 at test end. Additional samples were submitted for analysis as warranted. Test solutions were supplied to each replicate test chamber by a continuous flow metering system at a rate o f approximately 7.5 L per hour (approximately 24, 7.5-L test solution volume additions per day). Company Sanitized. Does not contain TSCA GEM -11 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 Embryos and alevins were held in relative darkness until day 39 and then held under a photoperiod o f 16 hours light and 8 hours darkness (which included 30 minutes o f transitional light between light and dark intervals) for the remainder of the study. After thinning, the fingerlings were fed newly-hatched brine shrimp (days 45-54) or newly-hatched brine shrimp and trout chow (days 55-89). Test solutions were maintained between 11.0 and 11.7C (mean 11.4C). Observations were made for assessment o f number of dead eggs, first and last day of hatching, first day of swim-up, survival and abnormalities from hatching to thinning, and survival and abnormalities from thinning to test end. Standard length and blotted wet weight o f surviving fingerlings were determined at test end. CONCLUSION: The NOEC (No Observable Effect Concentration) for H-24678 in a 90-day early life-stage toxicity study using Rainbow trout, Oncorhynchus mykiss, was 2.5 mg/L based on nominal H-24678 concentrations and egg hatching. The LOEC (lowest observed effect concentration) and MATC (maximum acceptable toxicant concentration), for the same endpoint were 5 and 3.5 mg/L, respectively. STUDY COMPLETION: (see report cover page) - 12- Company Sanitized. Does not contain TSCA Ci H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 IN T R O D U C T IO N The purpose of this study was to assess the early life-stage toxicity o f H-24678 to embryos, alevins, and fmgerlings o f rainbow trout, Oncorhynchus mykiss, in a flow-through, 90-day test. MATERIALS AND M ETHODS A. Test Guidelines Except as noted below, the study design complies with the following test guidelines: U.S. Environmental Protection Agency (EPA) (1982). TSCA EG-11, Fish Early Life-Stage Toxicity Test. U.S. Environmental Protection Agency (EPA) (1996). OPPTS 850.1400, Fish Early-Life Stage Toxicity Test. Organisation for Economic Co-Operation and Development (OECD) (1992). TG 210. Fish Early-Life Stage Toxicity Test. - A constant test target temperature o f 10 2C was used for the duration o f the test, not 10 2C for embryonic development and 12 2C for alevin and fingerling development (OECD 210). Based on the use o f existing temperature control equipment, which is typical o f that used in most facilities for fish early life-stage studies, the high probability o f exceeding the guideline limit for temperature variation obviated temperature adjustment in the middle of the study. This exception did not affect the objectives or the validity o f the study. The Certificate o f Analysis (COA) for the test substance expired prior to the initiation o f the study. However, prior to study initiation the study sponsor provided a letter indicating that the test substance was acceptable for study use and was stable for a greater period o f time than indicated on the COA. In addition, the stability o f H-24678 in HLWW under test conditions was verified based on the analytical recoveries o f the target analytes. Sanitized. Does not contain TSCA C Company H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 A master stock solution (Stock A) was prepared at a concentration o f approximately 100 mg/mL H-24678 in a 1:4 solution o f isopropyl alcohokHLWW. Secondary stock solutions were prepared by adding predetermined volumes o f Stock A to 500 mL.volumetric flasks and bringing the final volume to 500 mL with 100 mL o f isopropyl alcohol and the appropriate remaining volume o f HLWW. All stock solutions were prepared on a weekly basis throughout the study. The nominal IPA co-solvent concentration used during the test in any test substance or control solution did not exceed 0.1 mL/L. At test start, the stock solutions were clear with a yellow tint with no visible precipitate. Test solutions were clear and colorless with no visible precipitate at test start. A precipitate appeared in the test solutions on day 3 (nominal 5,10 mg/L), day 7 (nominal 2.5 mg/L), day 13 (nominal 1.3 mg/L) and day 19 (nominal 0.63 mg/L) that was present for the remainder of the study. The total amount o f H-24678 used in the definitive study was approximately 1418 grams. C. Dilution W ater Dilution water originated from the Haskell Laboratory well which is 378-feet deep and is cased and sealed to bedrock. The hardness o f the HLWW is adjusted to approximately 100-140 mg/L as CaCCh by the flow-proportioned addition o f CaCL. The HLWW is then aerated, passed through a green sand filter to remove iron, and filtered through 50-, 10-, and 0.45-pm cartridge filters to remove particulates. The water is heated or chilled as appropriate and distributed through aged polyvinyl chloride piping. The dilution water is analyzed twice yearly for major anions and cations, metals, total organochlorine and organophosphate pesticides, and polychlorinated biphenyls (Table 1). The dilution water meets OECD(3) and ASTM(4) criteria and specifications. D. Test O rganism Culture Rainbow trout, Oncorhynchus mykiss, unfertilized eggs and sperm were purchased from Troutlodge, Inc., Sumner, Washington. Eggs and sperm (collected from 3 female and 1 male brood fish) were received in separate plastic bags which were kept on ice (approximately 3.1C) during shipping. Eggs and sperm were allowed to equilibrate to approximately 11.5C in a water bath. Approximately 200 mL o f the buffer solution containing glycine, TRIS, and sodium chloride was added to the bag containing the eggs. The purpose o f the buffer was to remove any ovarian fluid that could impede fertilization. The bag was placed back into the water bath for approximately 15 minutes. The bag o f eggs was taken out o f the water bath and the buffer solution was removed. The bag o f sperm was removed from the water bath and its contents were added to the bag o f eggs. The bag was gently rocked back and forth to evenly distribute the sperm. Buffer solution (50 mL) was also added to the bag to help activate the sperm. The bag was again gently rocked back and forth and returned to the water bath for about 15 minutes. The embryos were removed from the waterbath and the buffer solution and sperm poured off o f the embryos. The embryos were then rinsed with cold HLWW to remove residual sperm and other debris before being transferred to two stainless steel tanks, each containing 10 L o f aerated HLWW, in a water bath (11.5-11.6C) under darkness. Approximately 22.5 hours later, embryos were randomly placed into embryo cups to begin the test. The embryonic stage at the beginning - 14- Company Sanitized. Does not contain TSCA CBS H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 of exposure was verified by selecting a representative sample o f fertilized eggs, photographing, and comparing with literature sources/5'1 Embryos and larvae were identified by labels on aquaria and test chambers. E. T est M ethods(1,2,3) Five test substance concentrations, a dilution water control, and an isopropyl alcohol (IPA) control were used in this study. Nominal test substance concentrations were 0.63, 1.3, 2.5, 5, and 10 mg/L. The concentration o f IPA in all test solutions, with the exception o f the dilution water control, was <0.1 mL/L. Test chambers (2 replicate chambers per concentration) were 13-L stainless steel aquaria (30 [length] x 14.5 [width] x 30 [height] cm), each holding approximately 7.5 liters o f solution (16.5-cm liquid depth) and fitted with a screen mesh-covered overflow pipe. A recirculating waterbath was used to maintain temperature in the test chambers at 10 2C. Test chambers were positioned in the waterbath using random numbers. Test solutions were supplied to the test chambers under flow-through conditions by a continuous flow metering system. Metering system surfaces exposed to test solutions consisted o f glass, Tygon, and Teflon. The test substance metering system consisted o f a Rainin Rabbit-PlusTM peristaltic pump that delivered the stock solutions and IPA control continuously to the mixing chambers o f the test system at approximately 1.5-2.1 mL per hour. Test solutions in the mixing chamber were stirred by a mini mixer. Test solutions were delivered continuously from the mixing chamber to each replicate test chamber at approximately 7.5 L per replicate per hour (approximately 24 test solution volume additions per day). Checks o f the flow rate into each replicate chamber were made prior to test start and after test end. Two 212-mL glass embryo cups (5.5-cm diameter) with screen mesh bottoms attached with silicone adhesive were suspended in each replicate. Twenty embryos were placed into each embryo cup (total o f 40 embryos per replicate and 80 embryos per test concentration). Embryo cups were assigned to each replicate and embryos were assigned to embryo cups using random numbers. Embryo cups oscillated at approximately 4 rpm (2 to 7-cm water depth in embryo cup). Embryo cups were inspected daily and unfertilized eggs and damaged or fungus-infected embryos were removed. The criterion for embryo death was opaque appearance. The surviving alevins and fingerlings were thinned to 15 per replicate using random numbers and released into the appropriate test chamber replicate on day 45 after swim-up had begun in the dilution water control. Fish that swim-up are those that occupy and are able to maintain their position in the top half of the water column and in which the yolk sac has mostly been resorbed. Test organisms thinned from the test chambers were humanly sacrificed with MS-222 and stored frozen for potential residue analysis at a later date. On day 45, feeding was initiated. The fingerlings were fed newly-hatched brine shrimp (Artemia sp., San Francisco Bay Brand, Newark, California) 3 times daily on weekdays and twice daily on weekends and holidays. On day 55 through day 89, one o f the daily feedings with brine shrimp was replaced by a feeding with Aqua Max Starter Fingerling 300 5D03 (PMI Nutrition International, LLC). The Sanitized. Does not contain TSCA CBl H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 f fmgerlings were last fed on day 89 (am, trout chow only), approximately 24 hours prior to test conclusion on the following day. Brine shrimp and Aqua Max are analyzed periodically to assure that contaminants are not present at levels that would be expected to affect the scientific integrity o f a study. Uneaten food and/or debris were removed as needed. The number o f live fmgerlings was determined daily by actual count. The criteria for alevin and fingerling death were the absence of opercular movement and lack o f reaction to gentle prodding. Surviving fmgerlings were humanely sacrificed using MS-222 at test end and measurements made of length and blotted wet weight. In addition, 3 fish from each test chamber were frozen for potential residue analysis at a later date. Diluter operation was checked twice daily. Test solution concentrations were verified by chemical analysis prior to beginning the test, at test start, weekly, and at test end. Before thinning, water samples were taken from a point between the 2 embryo cups in each replicate test chamber (2 samples per concentration plus back-up samples). After thinning, water samples were taken at mid-depth from each test chamber replicate (2 samples per concentration plus back-up samples). Test solutions were maintained between 11.0 and 11.7C (mean = 11.4C). The diluter was wrapped in black plastic to keep the embryos and alevins in relative darkness until the completion o f hatching. The black plastic was removed from day 39 onwards. A photoperiod o f 16 hours light (329 - 553 Lux) and 8 hours darkness, which included 30 minutes of transitional light (approximately 1 8 -1 5 1 Lux) between the light and dark intervals, was used throughout the remainder o f the study. Dissolved oxygen, pH, and temperature were measured in both replicates of the dilution water control and all test concentrations at the beginning of the test before embryos were added, weekly, and at test end. A continuously-recording thermometer was used to check temperature variations in the test system. Total alkalinity, EDTA hardness, and conductivity were measured in the A replicate o f the dilution water control, IPA control, and the highest test substance concentration with surviving test organisms at the beginning o f the test, weekly, and at test end. Test solutions were not aerated during the study. F. Sam ple Preparation and Chemical Analysis 1. Sample Collection and Treatment Analytical determination o f H-24678 concentrations in test solution samples was performed with samples collected from each replicate test chamber prior to test initiation, at test initiation, and weekly after test initiation until test termination. A primary sample plus a back-up sample was collected from each replicate test chamber of each control (dilution water control, isopropyl alcohol control) and test substance concentration. Aliquots from each of the primary samples were placed directly into an autosampler vial for analysis by LC/MS. The 5 mg/L and 10 mg/L samples were diluted before analysis with HLWW 1:2 and 1:4, respectively. The remaining primary sample solutions and backup samples were stored at room temperature. - 16- Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynckus mykiss DuPont-11603 2. Instrument and Conditions HPLC Instrument: MS Instrument: Model 2795, Waters Quattro Micro, Micromass (Day -1 to Day 7) and Waters ZQ (Day 11 to Day 90) LC Parameters: Column: Mobile Phase: Luna 3u C l8(2), 20 mm x 2mm A- 50 mM ammonium acetate in water* B- methanol Gradient: Time (min) %B Flow (mL/min) 0 30 0.25 5 100 0.25 10 100 0.25 10.1 30 0.50 12 30 0.50 12.1 30 0.25 * On several days o f analysis addition of 0.1 % or 0.4 % triethylamine (v/v) to the 50 mM ammonium acetate was required in order to obtain acceptable peak shapes for the Column Temperature: Injection Volume: Column Switch: 30C 50 pL 2 and 10 min M S Parameters: Ionization mode: Capillary voltage: Cone voltage: Source Temperature: Desolvation Temperature: Data Acquisition Function: Electrospray, negative ions 3.2 kV 30 V 120C 450C (pIR of 789 m/z; 0-10 min SIR o f 485 m/z; 0-10 min 'of 443 m/z; 0-10 min 3. Quantitation H-24678 is a mixture oil )ne chemical species was selected from each group and monitored by LC/MS though the study. The selected species chemical structures, names, and abbreviations used were as follows: Sanitized. Does no* contain TSCA CBI Compaq -17- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 species that were monitored during the study. The concentrations o f individual chemical species that were monitored during the study were represented as the total concentrations o f the H-24678. Stock solutions of the reference standard, were made by dissolving approximately 1 g of H-24678 in 2 mL isopropanorin a 10 mL volumetric flask, and bringing the volume to 10 mL with HLWW. Appropriate aliquots of the stock solution were diluted with dilution water (HLWW) to prepare calibration standard solutions with concentrations ranging from 0.258 to 5.25 mg/L. Dilution water was run as a zero (blank) calibration standard solution. Duplicate injections o f test and calibration standard solutions were made and peak areas were determined electronically. - 18- C om pany Sanit 1. D o e s nH*Ti stamTSCJ / `"N H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 The calibration standard curve was generated by regression analysis of the peak area data from chromatography of the calibration standard solutions. Data for test solutions were compared to the calibration standard curve to determine concentrations of each o f the monitored compounds. The limit o f detection (LOD) and limit of quantitation (LOQ) were determined by calculating the average noise level in chromatograms o f the water and IPA control solutions and comparing them to the signal of a calibration standard of known concentration. Three chromatograms were examined for noise-related peaks near the retention time o f the analyte. The LOD was calculated as 3 times the concentration equivalent of the mean noise level. The LOQ was calculated as 10 times the concentration equivalent of the mean noise level. G. Statistical Analysis Since there were two controls, water and IPA, for each endpoint, the two controls were compared using either the Mann-Whitney test or Fisher's Exact test. If there was no significant difference, the two controls were combined for all further analysis o f that endpoint. If there was a significant difference between the two controls, then the dilution water control was dropped from analysis for that endpoint and all further analysis was done using the IPA control only. The NOEC for each response was determined through the use of trend tests (Cochran-Armitage trend test or the Jonckheere trend test) wherever appropriate. These trend tests assume monotonicity in the concentration-response. Parametric, or if appropriate, non-parametric tests, were used to determine if there was significant departure from monotonicity. Other multiple comparison methods were used for non-monotone data. Hatching, mortality and larval abnormality data were evaluated by the Cochran-Armitage test(6) used in a step-down manner(7) with equally-spaced concentration scores. If there was significant departure from monotonicity, then Fisher's exact test was used with a Bonferonni correction.(7) Continuous or reproduction data (length, weight, first and last day of hatching, first day of swimup) were evaluated for normality using the Shapiro-Wilk test(8) while homogeneity of variance was assessed by Levene's test/91 Data found to be both normal and homogenous were evaluated in the context o f AN0VA.<7) Monotonie concentration-response data were evaluated using the Jonckheere-Terpstra trend test.(10) Non-monotonic concentration-response data were assessed using the Dunnett method(7) if the data were normally distributed and homogeneous; using the Tamhane-Dunnett (or T3) method(7) if the data were normally distributed but heterogeneous. If factors in addition to concentration were considered, such as the number of specimens in the test chamber at test start, then the Jonckheere test was not appropriate and multi-factor ANOVA was used for analysis. Non-normal data were analyzed by alternative methods.(9) If possible, a transformation was found to obtain normality. Otherwise, a KruskalWallis test(10) replaced the ANOVA, Dunn's test(7) replaced Dunnett's, and the JonckheereTerpstra test was still applicable, except as noted. Outliers were determined by the Tukey outlier rule and, if present, their effect on the conclusions was determined. If massive ties were present in a continuous response, exact(n) permutation methods were used for data analysis. An exact version o f the Kruskal-Wallis test was used, followed by exact Sanitized. Does not contain TSCA CBI company - 19- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 versions of Jonckheere-Terpstra or separate Mann-Whitney50) (or, equivalently, Wilcoxon) comparisons o f each treatment group to the dilution water control, using a Bonferonni correction. Wherever possible, determination o f the EC50 was done by probit analysis for all frequency responses, adjusting for dilution water control mortality. Where probit analysis failed, a movingaverage angle or binary estimation procedure0 was used for analysis. Inverse regression0 3) was used to estimate the LC50 for length and other continuous responses, if appropriate. The No Observable Effect Concentration (NOEC) was defined as the highest measured concentration at or below which no statistically significant effect was observed. The Lowest Observed Effect Concentration (LOEC) was the lowest measured concentration at which a statistically significant effect occurred relative to the dilution water control. The maximum acceptable toxicant concentration (MATC) was defined as the geometric mean of the NOEC and LOEC. RESULTS A. Analytical Report 1. Chromatographic Results pluted as a well-resolved peak with a retention time o f approximately 6.7 m in u te s .V H M B I H H |B |S ^ n te d a s a well-resolved peak with a retention time of approximately 5.8 m i n u t e s f p B H H H H r l u t e d as a well-resolved peak with a retention time o f approximately 5.3 minutes. Figure 2 shows a representative calibration curve obtained for JjA chromatogram of a typical standardls shown in Figure 3. Figures 4, 5 and 6 show chromatograms o f a dilution water control solution, IPA control solution, and test concentration solution, respectively. The LOD and LOQ for t h e p P P ^ H ^ ^ ^ v e r e determined to b^Q.0022 mg/L and 0.0072 mg/L of H-24678, respectively. Th^LOD and LOQ for detm nine(N obe(U ^9 mg/L and 0.20 mg/L o f H-24678, respectivSI^TT i^LO ^tncuroQ for t h e M H l H H H n v e r e determined to be 0.042 mg/L and 0.14 mg/L o f H-24678, respectively. 2. Test Solution Results The mean, measured replicate concentrations o f H-24678 for eg ?h monitored species and sampling day are shown in Table 2. Mean measured values oi Tanged fron\99 to 121% o f the targeted nominal concentrations. Mean measured values oi 77 to 100% o f the targeted nominal concentrations. Mean measured values"of ranged from 95 to 110% o f the targeted nominal concentrations. These data indicate that the H-24678 concentrations were maintained at acceptable levels throughout the definitive test. -20- Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss Control solutions showed no detectable concentrations o f H-24678 (Table 2). DuPont-11603 B. In-Life Report Selection of test concentrations for the 90-day trout early-life stage study were based on a previous study/!4) Nominal H-24678 concentrations selected for the 90-day trout early life-stage study were 0.63, 1.3, 2.5, 5, and 10jng/L. The corresponding mean, measured concentrations o f H-24678 from 0.75,1 .^ 2 .8 , 5.6, and 10 mg/L. The corresponding mean, measured concentrations o f H-24678 2.1,4.5, and 10 mg/L. The corresponding mean, measured concentrations ofiH::24678 f r o m |H 0 f t H H f l H n a l y s e s were 0.63,1.3, 2.7, 5.0, and 11 mg/L. A dilution water control and anPA c o n tro ^ e re used in this study. The dilution water control solutions showed no detectable levels o f H-24678. Dilution water quality was acceptable based on OECD(4) and ASTMi5) dilution water criteria. Based on the most recent semi-annual dilution water analysis (Table 1), contaminant concentrations were below concentrations that could be expected to affect the integrity o f a study. All chemical and physical parameters in the early life-stage study were within expected ranges (Tables 3 to 6). Temperature and pH of the test substance and dilution water control solutions ranged from 11.0 to 11.7C (mean 11.4C) and 7.1 to 7.7, respectively. Dissolved oxygen concentrations ranged between 8.1 and 10.9 mg/L (75 and 100% o f air saturation based on an air saturation value of 10.8 mg/L at 12C) over the duration of the study. The most recent analyses of brine shrimp and Aqua Max indicated that total organochloride pesticides and PCBs were below concentrations that would be expected to affect the scientific integrity o f the study. On test day 87, a clog in the test solution delivery tube for replicate test chamber B o f the nominal 10 mg/L test concentration resulted in the death o f 12 o f the 15 fish in this test chamber. The deaths were caused by the low dissolved oxygen concentration (1.8 mg/L) in this test chamber resulting from the lack of flow to the chamber and were not related to the effects of the test substance. The fact that this mortality was not related to the test substance exposure was confirmed by the survival o f 15 of 15 fish through the end o f the study (day 90) in the replicate A test chamber of the nominal 10 mg/L test concentration. Flow was re-established to the replicate B test chamber on day 87 and the remaining 3 live fish also survived through the end of the study. The length and blotted wet weight were measured for the 12 fish that died on day 87. These data were combined with the data for the 3 remaining fish that survived through the end of the study in all statistical analyses o f survival, length, and wet weights. A summary of hatching, swim-up, survival, and abnormalities from hatching to thinning is presented in Table 7. A summary o f survival and abnormalities from thinning to test end, and mean length and wet weight (blotted dry) values at test end is presented in Table 8. Biological observations are presented in Tables 9 to 36. A photograph of a representative fertilized egg used to start the study is presented in Figure 1. Loadings in the A and B replicates o f the dilution water control at the end o f the test were 0.1093 and 0.1105 g fish per liter passing through each companv Sanitized- Does no. contain TSCA CBi -21 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynckus mykiss DuPont-11603 test chamber in 24 hours based on a daily turnover rate o f 180 L or approximately 24, 7.5-L test solution volumes. C. Statistical Analysis Report The NOEC for number o f dead eggs was 2.5 mg/L by the Cochran-Armitage trend test; no meaningful EC50 could be calculated for this endpoint. Larval survival and abnormality NOECs were determined using Cochran-Armitage trend tests. The NOECs for larval survival from hatch to thinning and from thinning to test end exceeded the highest test concentration o f 10 mg/L. Observed mortality at thinning was 4% or less in each dose group and no additional test substance-related mortality occurred between thinning and test end. The NOEC for presence o f larval abnormalities at thinning exceeded 10 mg/L. The NOEC for presence o f larval abnormalities at test end was 5 mg/L. No meaningful estimate o f an EC50 could be obtained for these endpoints. First and last day of hatching and first day of swimup exhibited massive ties (a large number of observations having the same value), and an exact analysis was conducted using StatXact. The first day of hatching and first day of swimup data displayed a monotone trend, so the NOEC was determined by an exact step-down Jonckheere-Terpstra trend test in each case. The NOECs for these two endpoints were greater than 10 mg/L. The last day o f hatching displayed a significant lack o f menotonicity so the NOEC o f 10 mg/L was determined using multiple exact MannWhitney tests. The step-down Jonckheere-Terpstra trend test determined the NOEC for length o f surviving trout at test end to be 0.63 mg/L. If length was analyzed using Dunnett's test, the NOEC was greater than the highest test concentration o f 10 mg/L. However, the Dunnett's test /-value for the 10 mg/L test concentration compared to the pooled control was only marginally greater than 0.05 (p=0.0573). The NOEC for weight at test end was 5 mg/L by the step-down Jonckheere-Terpstra trend test. -22- r-mmnanv Sanitized. P oes not contain TSCA CBl H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 DISCUSSION The mean measured concentrations of H-24678 were maintained at acceptable concentrations throughout the definitive study. The dilution water control and IPA control contained no detectable concentrations o f H-24678. The most sensitive endpoint during the study appeared to be length of surviving fish at the end of the study (NOEC = 0.63 mg/L) based on the results o f the Jonckheere-Terpstra trend test. This test assumes monotonicity in the test data. A test for monotonicity indicated that the data met this assumption with a p value of 0.04. However, visual evaluation suggests that the length data are not truly monotonic since the mean lengths for the dilution water control, IPA control, and 0.63,1.3, 2.5, 5, and 10 mg/L nominal H-24678 test concentrations, respectively, were 4.48, 4.34,4.26, 4.36, and 4.17 centimeters. A more traditional statistical analysis of these data suggests that the NOEC for length was greater than 10 mg/L although the analysis o f the effects at 10 mg/L just exceeded the 0.05 p value for significance with a p value o f 0.0573. Pooling of the control data (dilution water mean length = 4.54 cm, IPA = 4.42 cm) increased the power o f the trend test analysis resulting in the NOEC for length o f 0.63 mg/L as opposed to the results o f Dunnett's test. Comparison o f only the IPA control length versus the lengths from the test concentrations indicated the NOEC for length was greater than 10 mg/L. Based on this analysis, the presence of IPA in all test concentrations, and the observation that length in the 5 mg/L treatment did not fit a monotonic trend, the Jonckheere-Terpstra trend test NOEC for length is not truly reflective o f the test data. The percent decrease in length, relative to the pooled control data, for the 0.63, 1.3, 2.5, 5, and 10 mg/L nominal H-24678 test concentrations was -3.2% , -4.9%, -4.9%, -2.8%, and -7.0% , respectively. The decrease in length, as a percent o f the pooled control data, also seems o f doubtful biological significance at nominal test concentrations below 10 mg/L H-24678. The most biologically relevant endpoint for the study appears to be the NOEC o f 2.5 mg/L for the number of dead eggs. CONCLUSION The NOEC (no observable effect concentration) for H-24678 in a 90-day early life-stage toxicity study using rainbow trout, Oncorhynchus mykiss, was 2.5 mg/L based on nominal H-24678 concentrations and number o f dead eggs. The LOEC (lowest observed effect concentration) and MATC (maximum acceptable toxicant concentration), for the same endpoint were 5 and 3.5 mg/L, respectively. Company Sanitized. Does not contain TSCA CBS H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 RECORDS AND SAMPLE STORAGE All data and records for analytical characterizations conducted by the sponsor will be retained by the sponsor. Specimens (if applicable), raw data, and the final report will be retained at Haskell Laboratory, Newark, Delaware, or at Iron Mountain Records Management, Wilmington, Delaware. Company Sanitized. Does not contain TSCA CBS H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 REFERENCES 1. U.S. Environmental Protection Agency (EPA) (1982). TSCA EG-11, Fish Early Life-Stage Toxicity Test. 2. U.S. Environmental Protection Agency (EPA) (1996). OPPTS 850.1400, Fish Early-Life Stage Toxicity Test. 3. Organisation for Economic Co-Operation and Development (OECD) (1992). Guideline for the Testing o f Chemicals, Number 210: Early -Life Stage Toxicity Test. 4. American Society for Testing and Materials (ASTM) (1992). Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes. E1241-92. Annual Book o f ASTM Standards, Vol. 11.04. 5. Leitritz, E. and Lewis, R.C. (1976). Trout and salmon culture (hatchery methods). California Department o f Fish and Game, Fish Bulletin 164. 197 pp. 6. Agresti, A. 1990. Categorical Data Analysis, Wiley, New York. 7. Hochberg, Y. and Tamhane, A.C. (1987). Multiple Comparison Procedures, Wiley, New York. 8. Shapiro, S.S. and Wilk., M.B. (1965). An analysis o f variance test for normality (complete samples). Biometrika 52, 591-611. 9. Box, G.E.P. (1953). Non-normality and tests on variances. Biometrika 40, 318-335. 10. Lehmann, E.I. (1975). Nonparametrics: Statistical Methods Based on Ranks. Holden-Day, San Francisco. 11. Mehta, C. and Patel, N. (1995). StatXact 3 for Windows. Cytel Software Corporation, Cambridge. 12. Stephan, C. E. (1976). Methods for estimating an LC50, Aquatic Toxicology and Hazard Evaluation, Proceedings of the First Annual Symposium on Aquatic Toxicology, ASTM Special Technical Publication 634, American Society for Testing and Materials, Philadelphia. 13. Draper, N.R. and Smith, H. (1981). Applied Regression Analysis, 2nd edition, pp 266-273. Wiley, New York. 14. DuPont Haskell Laboratory (19( >). Acute Toxicii gairdneri). Unpublished reportJ 230 to Rainbow Trout (Salmo -25- Company Sanitized. Does not contain TSCA CBi H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLES -26- Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 u f p M ! s0 "Pw|l|4 2 3 -mi TABLE 1 CHEMICAL CHARACTERISTICS OF HASKELL LABORATORY WELL WATER3 Parameter MDL b Analytical Value Parameter MDL b Analytical Value BOD, mg/L 3.6 NDc Lead, mg/L 0.0089 ND COD, mg/L 1.7 ND Magnesium, mg/L 0.0195 3.92 DOC, mg/L 0.6 0.55 d Manganese, mg/L 0.0005 0.0037 J e TOC, mg/L 0.5 0.691 J MBAS/LAS, mg/L 0.035 ND Total Kjeldahl N, mg/L 0.3 ND Mercury, mg/L 0.000079 ND Ammonia N, mg/L 0.02 0.036 J Nickel, mg/L 0.0019 ND Turbidity, NTU 0.059 0.096 J Nitrite, mg/L 0.015 ND Phenolics, mg/L 0.012 ND Nitrate, mg/L 0.04 ND Color, apparent Co/Ptf 5.0 ND Ortho-phosphate, mg/L 0.0066 ND Solids Potassium, mg/L 0.0472 2.56 total suspended, mg/L 1.5 ND Selenium, mg/L 0.0011 ND Aluminum, mg/L 0.0477 ND Silver, mg/L 0.00005 ND Antimony, mg/L 0.0099 ND Sodium, mg/L 0.25 8.25 Arsenic, mg/L 0.0014 ND Sulfate, mg/L 1.5 8.0 Beryllium, mg/L 0.0005 ND Sulfide, mg/L 0.022 ND Boron, mg/L 0.0088 ND Zinc, mg/L 0.0049 ND Bromide, mg/L 2.0 ND Ca/Mg NA 8 10.41 Cadmium, mg/L 0.00008 ND Na/K NA 3.22 Calcium, mg/L 0.0493 40.8 Volatile priority Chloride, mg/L 6.0 56.1 pollutants, pg/L 0.5-40 ND Chlorine, residual, mg/L 0.04 ND Acid extractable Chromium, mg/L 0.00092 ND priority pollutants, pg/L 1.0-19 ND Cobalt, mg/L 0.0017 ND Base/neutral Copper, mg/L 0.0016 0.0043 J priority pollutants, pg/L 1.0-19 ND Cyanide, mg/L Iron, mg/L 0.004 0.0349 ND Pesticides/PCBs, pg/L ND Organophosphate 0.0019-0.29 ND Fluoride, mg/L 0.4 0.56 pesticides, pg/L 0.38-0.57 ND a Sample analyses performed at Lancaster Laboratories, Lancaster, Pennsylvania, date of sample collection 06 November 2002 unless indicated otherwise, MDL = method detection limit,c ND indicates not detected at the MDL,dValue corrected for the filter blank,eA "J" follows analytical values which were greater than the MDL but less than the limit of quantitation,f Units based on cobalt/platinum reference,8NA = not applicable. -27- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 2 MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 (mg/L) D ay-lb Day 0 Day 7 f Day 11 Day 13 Day 20 Day 27 Water Control A Water Control B IPA Control A IPA Control B 0.63 A 0.63 B 1.3 A 1.3 B 2.5 A 2.5 B 5.0 A 5.0 B 10 A 10B NDC ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 0.94 0.88 0.53 0.72 0.70 0.73 0.68 0.98 0.96 0.49 0.76 0.69 0.78 0.70 1.6 1.6 0.64 1.3 1.2 1.3 1.3 1.6 1.6 0.81 1.2 1.2 1.3 1.2 2.9 2.7 2.2 2.7 2.6 2.9 2.4 2.9 2.8 2.0 2.7 2.8 2.9 2.5 5.2 5.6 3.8 5.2 5.4 6.2 5.7 5.6 5.9 1.9 5.3 5.2 5.5 5.9 12 11 4.8 10 9.8 12 10 12 10 4.1 9.7 9.6 11 11 Company Sanitized. Does not contain TSGA CBf -28- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration3 (mg/L) Day 34 Day 41 (Ng,T) Day 48 h Day 55 Day 62 Day 69 Day 76 Water Control A Water Control B IPA Control A IPA Control B 0.63 A 0.63 B 1.3 A 1.3 B 2.5 A 2.5 B 5.0 A 5.0 B 10A 10B ND ND ND ND ND ND ND ND ND ND ND ND 0.85 0.80 0.69 0.81 0.75 0.69 1.3 1.5 1.5 1.3 1.6 1.6 2.1 3.2 (3.4)s 3.2 2.2 3.2 (3.8)s 3.1 6.0 5.4 5.3 5.3 5.5 5.3 7.1 9.5 10 8.1 10 9.7 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 1.0 0.69* 0.92 0.67 1.0 0.69* 0.76 0.62 1.5 1.1* 1.7 1.4 1.5 l.T 1.4 1.4 2.9 2.2* 4.0 2.3 2.9 2.5* 5.4 2.3 5.6 4.5* 8.9 5.5 5.5 4.7* 9.2 5.7 13 9.2 10 11 13 9.3 13 12 0* soi TSC -29- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss D u P o n t- 11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration3 (mg/L) " Concentration Day 83 Day 9 concentration (mg/L>).d Mean Percent Recovery6 Water Control A Water Control B ND ND ND ND IPA Control A IPA Control B ND ND ND ND 0.63 A 0.63 B 0.69 0.79 0.63 0.73 0.76 0.74 121 117 1.3 A 1.3 B 1.4j 1.2 1.3J 1.1 1.3 1.3 100 100 2.5 A 2.5 B 2.1 2.4 2.0 2.5 2.7 2.9 108 116 5.0 A 5.0 B 5.7 5.4 5.8 5.2 5.6 5.5 112 110 10 A 11 10 9.9 10B 11 8.8k 10 99 100 a Defined in terms of H-24678 mg/L. b Measured values prior to study start were used to verify system function but were not used to calculate mean measured test concentrations of H - 2 4 7 f L ^ ^ ^ j c ND denotes not detected. The limit of detection fo |B B B B B B i[M w a s 0.002^ng/L o f H-24678. LOQ denotes below limit of quantitation. The limiro^uantiration f o n H H ^ H H H K e a s 0.0072 mg/L o f ^ 4 6 7 ^ ^ ^ * d Mean, m easu red fl^ fjH H H tton cen tration was calculated as the average of values obtained from day 0 to day 90. * e Rased on nominal concentration. f Sampleswereanjdvzed one day after submission. This impacted the recoveries observed for I flp H H H H H H flsp ec ia lly at higher nominal concentrations, g *^AdditionaRampies were submitted due to partially clogged delivery line. The results in brackets represent samples taken after removal of the clog, h Reported data is the average o f two runs of the day 48 samples, i Reported dat^^ h^ verageoftw o runs of the day 62 samples. j There was n o fljJ ^ ^ H H H H e te c te d in 1.3 mg/L samples (replicate A and B) submitted on day 83. The results in tn^aol^represent data obtained for 1.3 mg/L samples submitted on day 84. k Additionally, samples for this replicate were submitted on day 87 and day 88. The results of the analysis were: 3.6 and 4.1, respectively. The deliveiy tube to this replicate was clogged on day 87. (Company Sanitized. Does not contain TSCA CBl -30- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration3 (mg/L) D ay-lb MeasuredHHH Concentration (mg/L) Day 0 Day 7 * Day 11 Day 13 Day 20 Day 27 Water Control A NDC ND ND ND ND ND ND Water Control B ND ND ND ND ND ND ND IPA Control A ND ND ND ND ND ND ND IPA Control B ND ND ND ND ND ND ND 0.63 A 0.63 B 0.43 0.35 0.51 0.47 0.40 0.39 0.36 0.67 0.40 0.37 0.44 0.34 0.33 0.32 1.3 A 1.3 B 2.5 A 2.5 B 0.96 0.58 0.82 0.87 0.75 0.77 0.81 0.85 0.68 0.82 0.85 0.94 0.73 0.57 2.1 1.2 1.6 1.7 1.4 1.3 1.3 1.9 1.2 1.8 1.9 1.8 1.6 1.4 5.0 A 5.0 B 10 A 10B 5.2 2.4 3.3 4.4 4.6 4.0 3.5 3.5 3.3 3.9 3.4 3.6 3.0 3.0 8.5 5.5 8.6 8.5 8.0 8.9 10 9.4 5.8 5.6 8.1 7.1 7.5 7.3 Sanitized. Does not contain TSCA CBf Company -31 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration3 (mg/L) Water Control A Water Control B IPA Control A IPA Control B 0.63 A 0.63 B 1.3 A 1.3 B 2.5 A 2.5 B 5:9 A 5.0 B 10 A 10B Day 34 ND ND ND ND 0.36 0.35 0.69 0.60 1.0 1.3 3.9 3.2 7.0 7.1 M easurecfl|PH Concentration (mg/L) Day 41 Day 4 8 " Day 55 Day 62 Day 69 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 0.66 0.53 0.98 0.60* 0.67 0.42 0.54 0.93 0.54` 0.66 1.5 1.3 1.5 1.3* 1.1 1.4 1.3 2.0 1.1* 1.0 3.0 (2.7)s 1.9 (3.1)g 2.5 2.4 3.8 2.6* 2.5 2.6 2.8' 2.6 5.1 5.4 5.8 5.6* 5.0 5.8 5.1 5.7 6.2` 4.6 12 11 15 9.9* 10 11 13 13 11` 8.6 Day 76 ND ND ND ND 0.68 0.59 1.4 1.2 2.0 2.3 6.8 5.8 11 9.9 Company Sanitized. D ees not eontaln TSCA BBI -32- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss D u P o n t-11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration3 (mg/L) Measured ancentration Tmg/L) Day 83 Day 90 C oncentration (mg/L)d Mean Percent Recovery3 Water Control A Water Control B ND ND ND ND IPA Control A IPA Control B ND ND ND ND 0.63 A 0.63 B 1.3 A 1.3 B 2.5 A 2.5 B 5.0 A 5.0 B 0.79 0.72 0.60 0.66 1.2j 1.1 1.3j 1.1 2.3 1.9 2.5 2.1 5.5 2.6 6.4 4.7 0.57 0.50 1.0 1.0 2.1 2.1 4.5 4.5 90 79 77 77 84 84 90 90 10 A 11 13 10 10B 11 8.9k 9.0 100 90 a Defined in terms o f H-24678 mg/L. b Measured values prior to study start were used to verify system function but were not used to calculate mean measured test concentrations of H-24 c ND denotes not detected. The limit of detection foil H-24678. LOQ denotes below limit of quantitation.'T'he limit of quantitat was 0.20 mg/L o ^ jW 4 6 7 8 ^ ^ ^ ^ d Mean, m easu red fl^ flH flf^ H xm cen tration was calculated as the average of values obtained from day 0 to d a y 9 0 ^ ^ ^ ^ ^ ^ ^ * e Based on nominal concentration, f Samples were analyzed one day after submission. g Additional samples were submitted due to partially clogged delivery line. The results in brackets represent samples taken after removal of the clog, h Reported data is the average o f two runs of the day 48 samples, i Reported datq^s the average of twojuns of the day 62 samples. j There was n o H H J H H H H H H le te c te d in 1.3 mg/L samples (replicate A and B) submitted on day 83. The ^ u ltS ra ^ D lS r ep resen t data obtained for 1.3 mg/L samples submitted on day 84. k Additionally, samples for this replicate were submitted on day 87 and day 88. The results o f the analysis were: 8.9 and 8.7, respectively. The delivery tube to this replicate was clogged on day 87. Company Sanitized. Does;ni ot contain TSCA CBl -33- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration3 (mg/L) Water Control A Water Control B IPA Control A IPA Control B 0.63 A 0.63 B 1.3 A 1.3 B 2.5 A 2.5 B 5.0 A 5.0 B 10 A 10B D ay-lb NDC ND ND ND 0.73 0.72 1.4 1.4 2.7 2.6 5.3 5.4 11 11 M easureJmi oncentration DayO 7r Day 7 f Day 11 Day 13 Day 20 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 0.65 0.53 0.64 0.57 0.53 0.71 0.47 0.64 0.56 0.48 1.3 1.0 1.2 1.1 1.1 1.3 0.44 1.2 1.0 1.1 2.4 2.6 2.6 2.4 2.2 2.4 2.5 2.6 2.6 2.4 4.8 4.7 4.5 5.1 5.3 5.0 4.7 4.4 4.5 4.6 10 9.3 11 12 11 10 10 11 11 10 Day 27 ND ND ND ND 0.51 0.38 1.0 0.88 2.0 2.4 5.0 5.1 12 9.8 -34- Company Sanitized. Does not contain TSCA CBt H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration" (mg/L) Water Control A Water Control B IPA Control A IPA Control B 0.63 A 0.63 B 1.3 A 1.3 B 2.5 A 2.5 B 5.0 A 5.0 B 10 A 10 B MeasuredJjj^H Concentration Day 34 1 Day 41 Day 48 h Day 55 Day 62 Day 69 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 0.39 0.56 0.97 1.25 m - 0.30 0.67 0.80 l . l 1 m .. 1.2 1.6 1.5 2.4' 0.90 - 1.2 1.6 1.5 2.41 0.60 0.85 (2.9)s 3.4 - 0.68 (2.9) 3.2 3.1 4.8' 4.8 3.2 4.8' 3.0 6.1 4.0 5.8 4.7' 5.8 - 6.5 2.5 5.5 4.0' 5.6 15 10 15 8.2' 10 - 14 12 15 7.31 11 Day 76 ND ND ND ND 0.67 0.68 1.3 1.4 2.1 2.1 5.2 4.9 10 10 Sanitized. Does net contain T S C A C B I Company -35- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration (mg/L) M easurecS m m m Concentration _________ (mg/L)_________ Day 83 Day 90 Water Control A Water Control B ND ND ND ND IPA Control A IPA Control B ND ND ND ND 0.63 A 0.63 B 0.68 0.63 0.61 0.44 1.3 A 1.3 B 2.4J 0.74 2.3J 1.1 2.5 A 2.5 B 2.4 2.3 2.5 2.4 5.0 A 5.0 B 5.5 4.2 5.4 5.0 00 l/t 10 A 11 11 10B 11 (mg/L)d -- -- -- 0.66 0.60 1.3 1.3 2.7 2.6 5.1 4.8 11 11 Mean Percent Recovery6 -- ____ -- 105 95 100 100 108 104 102 96 110 110 Sanitized. Does not contain TSC CBl Compaq -36- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS a Defined in terms of H-24678 mg/L. b Measured values prior to study start were used to verify system function but were not used to calculate mean measured test concentrations of H -2-^78._________ c ND denotes not detected. The limit of detection f o r |^ [ ^ ^ ^ ^ ^ ^ P vas0.042 mg/L of H-24678. LOQ denotes below limit o f quantitation. The limit of quantitation forj^ ^ H H H H H n v a s 0.14 mg/L of H - ^ 6 7 ^ ^ ^ ( | " d Mean, m ea su reJ B ^ J ((((B B o n cen tra tio n was calculated as the average of values obtained from day 0 to day 90. e Based on nominal concentration. . f Samples were analyzed one day after submission. g Additional samples were submitted due to partially clogged delivery line. The results in brackets represent samples taken after removal of t h ^ lo g ^ ^ ^ ^ t L h The analysis failed on day 48 samples f o r f l ^ ^ ^ f ^ H T h e reported data represent reanalysis of day 48 samples with modified mobile phase to allow quantitation ofj i Reported datM ^jh^verae^fcwo runs of the day 62 samples. j There was n o H m m ^ H B e t e c t e d in 1.3 mg/L samples (replicate A and B) submitted on day 83. The results in the table represent data obtained for 1.3 mg/L samples submitted on day 84. k Additionally, samples for this replicate were submitted on day 87 and day 88. The results of the analysis were: 1.6 and 8.0, respectively. The delivery jub^cMhiyjenlicate was clogged on day 87. 1 Problems with analytical method v ^ ^ j u ^ ^ ^ ^ M D r H m m ^ ^ i o m p o n e n t . No data was obtained for this set of samples for^^^BHBII^^Bo'ffinonent. m Sensitivity of the method was not sufficient to quantity th e^ ^ ^ B B U B M F 01' the 0.63 mg/L samples. TSGACBi company Sanitized. Doe no* contain -37- / H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 3 WATER CHEMISTRY OF THE DILUTION WATER CONTROL, IPA CONTROL, AND HIGHEST TEST CONCENTRATION WITH SURVIVING ORGANISMS4 Replicate Total Alkalinity Day (mg/L as CaCOs) Dilution Water Control 0A 51 6A 50 13 A 51 20 A 51 27 A 49 34 A 50 41 A 51 48 A 51 55 A 53 62 A 51 69 A 54 76 A 51 83 A 49 90 A 52 IPA Control 0A 6A 13 A 20 A 27 A 34 A 41 A 48 A 55 A 62 A 69 A 76 A 83 A 90 A 49 5T 51 50 50 51 51 50 51 51 54 51 50 52 EDTA Hardness (mg/L as CaCOs) 127 120 130 127 128 126 123 123 125 123 122 132 118 122 127 120 130 127 129 127 122 123 127 123 125 130 118 122 Conductivity (pmhos/cm) 230 245 235 235 235 235 225 215 250 270 265 280 235 235 235 240 235 235 240 235 225 215 250 270 265 283 235 235 Company Sanitized. Does not contain I S 1 -38- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 3 (Continued) WATER CHEMISTRY OF THE DILUTION WATER CONTROL, IPA CONTROL, AND HIGHEST TEST CONCENTRATION WITH SURVIVING ORGANISMS1 Replicate Total Alkalinity EDTA Hardness Conductivity Day (mg/L as CaC03) (mg/L as CaCOs) (pmhos/cm) Highest Test Concentration with Surviving Organisms - 10 mg/L 0A 6A 13 A 20 A 27 A 34 A 41 A 48 A 55 A 62 A 69 A 76 A 83 A 90 A 51 51 51 51 50 51 50 50 51 53 56 50 54 52 127 230 119 240 129 235 127 235 127 240 126 235 126 220 128 215 125 250 123 270 128 260 130 280 119 235 125 235 a Based on nominal test concentrations. Company Sanitized. Does not contain TSCA CBl -39- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 4 TEMPERATURE (C) OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dilution Temperature (C) Water IPA Nominal Concentrations (mg/L) Day Replicate Control Control 0.63 1.3 2.5 5_____ 10 0A 0B 6A 6B 13 A 13 B 20 A 20 B 27 A 27 B 34 A 34 B 41 A 41 B 48 A 48 B 55 A 55 B 62 A 62 B 69 A 69 B 76 A 76 B 83 A 83 B 90 A 90 B 11.7 11.7 11.7 11.7 11.6 11.6 11.7 11.7 11.7 11.7 11.7 11.7 11.6 11.7 11.7 11.6 11.6 11.7 11.6 11.6 11.7 11.7 11.6 11.6 11.7 11.6 11.6 11.7 11.6 11.6 11.6 11.6 11.5 11.6 11.6 11.6 11.7 11.6 11.7 11.5 11.6 11.6 11.6 11.6 11.5 11.6 11.5 11.6 11.6 11.6 11.5 11.5 11.6 11.6 11.6 11.6 11.6 11.5 11.5 11.6 11.5 11.6 11.6 11.5 11.5 11.5 11.5 11.6 11.5 11.6 11.4 11.4 11.4 11.4 11.4 11.4 11.4 11.4 11.4 11.4 11.4 11.4 11.4 11.4 11.6 11.6 11.6 11.6 11.6 11.6 11.6 11.6 11.5 11.5 11.6 11.6 11.6 11.6 11.3 11.2 11.2 11.2 11.2 11.2 11.2 11.3 11.2 11.1 11.2 11.2 11.1 11.1 11.2 11.2 11.1 11.2 11.2 11.1 11.2 11.2 11.1 11.1 11.2 11.1 11.2 11.3 11.5 11.5 11.5 11.6 11.5 11.5 11.5 11.5 11.5 11.5 11.6 11.5 11.5 11.6 11.1 11.0 11.0 11.0 11.0 11.0 11.0 11.1 11.0 11.0 11.0 11.1 11.0 11.0 11.3 11.3 11.2 11.3 11.3 11.2 11.3 11.3 11.2 11.2 11.3 11.2 11.2 11.3 11.5 11.4 11.4 11.4 11.4 11.4 11.5 11.5 11.4 11.4 11.4 11.4 11.4 11.4 11.3 11.3 11.3 11.3 11.2 11.3 11.3 11.3 11.3 11.3 11.3 11.2 11.2 11.3 Tem perature (C) Mean for all concentrations, including controls 11.4 Std. Dev. of for all concentrations, including controls 0.2 Minimum for all concentrations, including controls 11.0 M TM u m,.for all concentrations, in c lu d i^ c<3infrglfeo^ l si^ [,ontaln TSCA CBi -40- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 5 pH OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dilution pH Water IPA ________ Nominal Concentrations (mg/L) Day Replicate Control Control 0.63 1.3 2.5 5 10 0A 7.4 7.4 7.5 7.5 7.5 7.5 7.5 0B 7.4 7.4 7.5 7.4 7.5 7.5 7.5 6A 7.7 7.7 7.7 7.7 7.7 7.7 7.7 6B 7.7 7.7 7.7 7.7 7.7 7.7 7.7 13 A 7.6 7.6 7.6 7.6 7.6 7.6 7.5 13 B 7.6 7.6 7.6 7.6 7.6 7.5 7.6 20 A 7.7 7.7 7.6 7.6 7.6 7.6 7.6 20 B 7.7 7.7 7.6 7.6 7.6 7.6 7.6 27 A 7.7 7.7 7.7 7.7 7.7 7.7 7.6 27 B 7.7 7.7 7.7 7.7 7.7 7.6 7.6 34 A 7.7 7.7 7.7 7.7 7.7 7.6 7.6 34 B 7.7 7.7 7.7 7.7 7.7 7.6 7.6 41 A 7.5 7.5 7.5 7.5 7.5 7.4 7.4 41 B 7.5 7.5 7.5 7.5 7.4 7.4 7.4 48 A 7.5 7.6 7.6 7.6 7.6 7.5 7.5 48 B 7.5 7.6 7.6 7.6 7.6 7.5 7.5 55 A 7.4 7.4 7.5 7.5 7.5 7.5 7.5 55 B 7.4 7.4 7.5 7.5 7.5 7.5 7.5 62 A 7.5 7.5 7.5 7.5 7.5 7.5 7.4 62 B 7.5 7.5 7.5 7.5 7.5 7.5 7.4 69 A 7.5 7.5 7.5 7.5 7.5 7.5 7.5 69 B 7.5 7.5 7.5 7.5 7.5 7.5 7.5 76 A 7.3 7.3 7.4 7.4 7.4 7.4 7.4 76 B 7.3 7.3 7.4 7.4 7.4 7.4 7.4 83 A 7.3 7.3 7.3 7.3 7.3 7.3 7.2 83 B 7.3 7.3 7.3 7.3 7.3 7.3 7.2 90 A 7.1 7.1 7.1 7.1 7.1 7.1 7.1 90 B 7.1 7.1 7.1 7.1 7.1 7.1 7.1 Minimum for all concentrations, including controls 7.1 Maximum for all concentrations, including controls 7.7 -41 - Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 6 DISSOLVED OXYGEN CONCENTRATION (mg/L) OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dilution Water IPA Day Replicate Control Control Dissolved Oxygen Concentration (mg/L) Nominal Concentrations (mg/L) 0.63 1.3 2.5 5 10 0A 0B 6A 6B 13 A 13 B 20 A 20 B 27 A 27 B 34 A 34 B 41 A 41 B 48 A 48 B 55 A 55 B 62 A 62 B 69 A 69 B 76 A 76 B 83 A 83 B 90 A 90 B 10.7 10.6 10.6 10.5 10.6 10.6 10.5 10.6 10.7 10.6 10.5 10.6 10.5 10.5 10.8 10.8 10.8 10.8 10.5 10.5 10.7 10.8 10.8 10.8 10.8 10.7 10.5 10.7 10.6 10.6 10.4 10.4 10.5 9.7 9.9 10.6 10.6 10.5 10.4 10.4 9.8 10.1 10.9 10.9 10.6 10.6 10.5 10.0 9.5 10.9 10.9 10.7 10.6 10.5 10.0 9.7 10.5 10.6 10.3 10.2 10.2 9.8 8.8 10.6 10.6 10.4 10.3 10.2 9.9 8.9 10.6 10.6 10.5 10.2 10.2 9.9 9.3 10.5 10.6 10.5 10.2 10.1 9.8 9.2 10.4 10.4 10.3 9.9 9.9 9.8 9.0 10.4 10.4 10.3 9.9 9.9 9.8 9.1 10.6 10.5 10.3 10.2 10.2 9.9 9.8 10.6 10.5 10.3 10.2 10.2 10.0 9.8 10.5 10.5 10.3 10.3 10.0 9.7 9.1 10.5 10.4 10.3 10.3 10.1 9.9 9.2 10.4 10.3 10.3 10.0 9.8 9.7 8.8 10.4 10.4 10.3 10.2 9.9 9.5 9.0 10.6 10.5 10.3 10.1 10.3 9.5 9.1 10.6 10.5 10.3 10.1 10.2 9.5 9.2 10.2 9.9 9.8 9.6 9.4 9.7 8.8 10.2 9.9 9.8 9.5 9.6 9.4 9.0 9.7 9.6 9.4 9.7 9.2 8.5 8.1 9.8 9.4 9.5 9.7 9.3 8.7 8.4 10.7 10.7 10.4 10.0 9.6 9.6 9.2 10.7 10.6 10.4 10.0 10.0 9.5 9.4 Dissolved O xygen Concentration (mg/L) Mean for all concentrations, including controls 10.1 Std. Dev. for all concentrations, including controls 0.6 Minimum for all concentrations, including controls 8.1 Maximum for all concentrations including controls 10.9 contain TSCACBt Company Sanitized. Doe no* -42- )) ) H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss_______________________________________________________________________________________DuPont-11603 TABLE 7 SUMMARY OF HATCHING, SURVIVAL, ABNORMALITIES, AND SWIM-UP FROM HATCHING TO THINNING FOR THE 90-DAY RAINBOW TROUT, ONCORHYNCHUS MYKISS, EARLY LIFE-STAGE TOXICITY TEST Nominal H-24678 Concentrations (mg/L) Water Control Replicate 1 2 3 4 Overall Mean IPA Control 1 2 3 4 Overall Mean 0.63 1 2 3 4 Overall Mean Hatching Day First 25 27 26 26 26 26 26 25 26 26 26 26 26 25 26 Last 27 28 27 28 28 27 28 27 27 27 28 27 28 28 28 Percent Hatch Number Hatching 18 18 19 19 19 Total 20 20 20 20 20 Percent 90% 90% 95% 95% 93% Number Alive 18 18 18 19 18 Hatching to Thinning Survival Abnormalities Number Hatching 18 18 19 19 19 Percent 100% 100% 95% 100% 99% Number Affected 0 0 0 0 0 Number Alive 18 18 18 19 18 Percent 0% 0% 0% 0% 0% First Day of Swim-Up 40 40 40 40 40 18 20 90% 18 18 20 90% 18 17 20 85% 17 16 20 80% 16 17 20 86% 17 18 100% 18 100% 17 100% 16 100% 17 100% 0 0 0 0 0 18 0% 41 18 0% 40 17 0% 40 16 0% 40 17 0% 40 16 20 80% 16 16 100% 0 15 20 75% 15 15 100% 0 18 20 90% 18 18 100% 0 17 20 85% 17 17 100% 0 17 20 83% 17 17 100% 0 16 0% 40 15 0% 40 18 0% 40 17 0% 40 17 0% 40 Company Sanitized. Does not contain TSCA CBl -43- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 7 (Continued) SUMMARY OF HATCHING, SURVIVAL, ABNORMALITIES, AND SWIM-UP FROM HATCHING TO THINNING FOR THE 90-DAY Rainbow trout, ONCORHYNCHUS MYKISS, EARLY LIFE-STAGE TOXICITY TEST Nominal H-24678 Concentrations (mg/L) 1.3 Replicate 1 2 3 4 Overall Mean 2.5 1 2 3 4 Overall Mean 51 2 3 4 Overall Mean Hatching Day First 26 25 25 25 25 26 26 26 25 26 26 24 25 26 25 Last 29 27 27 27 28 29 28 28 28 28 28 27 27 28 28 Percent Hatch Number Hatching 17 18 17 18 18 Total 20 20 20 20 20 Percent 85% 90% 85% 90% 88% Number Alive 16 17 16 18 17 Hatching to Thinning Survival Abnormalities Number Hatching 17 18 17 18 18 Percent 94% 94% 94% 100% 96% Number Affected 0 0 0 0 0 Number Alive 16 17 16 18 17 Percent 0% 0% 0% 0% 0% First Day of Swim-Up 40 40 40 40 40 17 20 85% 17 17 100% 0 19 20 95% 19 19 100% 0 19 20 95% 19 19 100% 0 17 20 85% 17 17 100% 0 18 20 90% 18 18 100% 0 17 0% 40 19 0% 40 19 0% 40 17 0% 40 18 0% 40 14 20 70% 14 14 100% 0 12 20 60% 12 12 100% 0 15 20 75% 14 15 93% 0 14 20 70% 14 14 100% 0 14 20 69% 14 14 98% 0 14 0% 40 12 0% 40 14 0% 40 14 0% 40 14 0% 40 contain TSCACBt -44- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 7 (Continued) SUMMARY OF HATCHING, SURVIVAL, ABNORMALITIES, AND SWIM-UP FROM HATCHING TO THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Nominal H-24678 Concentrations ___ (m9/L) 10 Replicate 1 2 3 4 Overall Mean Hatching Day First 25 26 25 25 25 Last 27 27 27 27 27 Percent Hatch Number Hatching 16 15 14 14 15 Total 20 20 20 20 20 Percent 80% 75% 70% 70% 74% Number Alive 15 15 14 11 14 Hatching to Thinning Survival Abnormalities Number Hatching 16 15 14 14 15 Percent 94% 100% 100% 93% 97% Number Affected 0 0 0 0 0 Number Alive 15 15 14 13 14 Percent 0% 0% 0% 0% 0% First Day of Swim-Up 40 40 40 40 40 O Ca0O Omw g. ) H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss ) ) DuPont-11603 TABLE 8 A SUMMARY OF SURVIVAL, ABNORMALITIES, AND GROWTH FROM THINNING TO TEST END FOR THE 90-DAY RAINBOW TROUT, ONCORHYNCHUS MYKISS, EARLY LIFE-STAGE TOXICITY TEST Nominal H-24678 Concentrations (mg/L) Replicate Number Alive Water Control A B Overall Mean 15 15 15 IPA Control A B Overall Mean 15 15 15 0.63 A B Overall Mean 15 15 15 1.3 A 15 B 15 Overall Mean 15 Survival Total 15 15 15 15 15 15 15 15 15 15 15 15 Thinning to Test End Abnormalities Percent Number Affected Number Alive Percent 100% 100% 100% 0 0 0 15 0% 15 0% 15 0% 100% 100% 100% 0 0 0 15 0% 15 0% 15 0% 100% 100% 100% 1 0 1 15 7% 15 0% 15 3% 100% 100% 100% 0 1 1 15 0% 15 7% 15 3% Growth of Surviving Fingerlings Mean Standard Length Std. Dev. (cm) Mean W et Weight Std. Dev. (g) 4.51 0.31 4.57 + 0.37 4.54 0.34 1.3119 0.3599 1.3265 0.2845 1.3192 0.3244 4.47 0.39 4.37 + 0.41 4.42 0.40 1.2986 + 0.3626 1.3274 0.4556 1.3130 0.4117 4.37 0.49 4.31 0.56 4.34 0.52 1.2107 0.4449 1.2187 0.5838 1.2147 0.5190 4.27 0.49 4.25 + 0.51 4.26 0.50 1.1729 + 0.4884 1.1803 0.3829 1.1766 + 0.4388 Company Sanitized. Does not contain TSCA -46- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 8 (Continued) SUMMARY OF SURVIVAL, ABNORMALITIES, AND GROWTH FROM THINNING TO TEST END FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Nominal H-24678 Concentrations (mg/L) Replicate Number Alive 2.5 A 15 B 15 Overall Mean 15 5 A 15 B 15 Overall Mean 15 10 A 15 Ba 15 Overall Mean 15 Survival Total 15 15 15 15 15 15 15 15 15 Thinning to Test End Abnormalities Percent Number Affected Number Alive Percent 100% 100% 100% 0 0 0 15 0% 15 0% 15 0% 100% 100% 100% 0 1 1 15 0% 15 7% 15 3% 100% 100% 100% 3 0 2 15 20% 15 0% 15 10% Growth of Surviving Fingerlings Mean Standard Length Std. Dev. (cm) Mean W et Weight Std. Dev. (g) 4.35 0.43 4.18 0.53 4.26 0.48 1.1924 0.3929 1.1735 0.5481 1.1830 0.4769 4.37 0.47 4,35 0.52 4.36 0.50 1.2494 0.4192 1.2344 0.4277 1.2419 0.4235 4.25 0.50 4.08 0.35 4.17 0.43 1.1301 0.4911 1.1415 0.3634 1.1358 0.4320 99 12 of 15 fish died on day 87 due to a clogged test solution delivery tube and subsequent low dissolved oxygen. Since these deaths were not test substance related these fish are indicated as having survived to test end and were included in all subsequent data analyses. No0s* o St j 5* mo ueduioO H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 9 DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicatet Removed Removed Eggs Left Larvae Larvae 0 1A 0 0 2A 0 - 0 IB 0 - 0 2B 0 1 1A 0 _ 1 2A 0 - 1 IB 0 1 2B 0 - 2 1A 0 2 2A 0 2 IB 0 _ 2 2B 0 - 3 1A 0 - 3 2A 0 - 3 IB 0 - 3 2B 0 - 4 1A 0 - 4 2A 0 - 4 IB 0 - 4 2B 0 - 5 1A 0 - 5 2A 0 - 5 IB 0 - 5 2B 0 _ 6 1A 0 - 6 2A 0 - 6 IB 0 - 6 2B 0 - 7 1A 0 - 7 2A 0 - 7 IB 0 - 7 2B 0 - 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 - - - - - - - - - - - - - _ _ - _ - - - - - - - - - - - " - - - - - - - - - - -48- company Sanitized. Does not contain TSCACBl H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate ' Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 8 1A 0 8 2A 0 - 8 IB 0 - 8 2B 0 - 9 1A 0 - 9 2A 0 - 9 IB 0 - 9 2B 0 - 10 1A 0 - 10 2A 0 - 10 IB 0 - 10 2B 0 - 11 1A 0 - 11 2A 0 - 11 IB 0 - 11 2B 0 _ 12 1A 0 - 12 2A 0 - 12 IB 0 - 12 2B 0 - 13 1A 0 - 13 2A 0 - 13 IB 0 - 13 2B 0 - 14 1A 2 - 14 2A 2 - 14 IB 0 - 14 2B 1- 15 1A 0 - 15 2A 0 - 15 IB 0 - 15 2B 0 0 20 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 2 18 - 2 18 - 0 20 - 1 19 - 2 18 - 2 18 - 0 20 - 1 19 - _ _ - - Company Sanitized. Does not contain TSCA CBI -49- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicatet Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 16 1A 16 2A 16 IB 16 2B 17 1A 17 2A 17 IB 17 2B 18 1A 18 2A 18 IB 18 2B 19 1A 19 2A 19 IB 19 2B 20 1A 20 2A 20 IB 20 2B 21 1A 21 2A 21 IB 21 2B 22 1A 22 2A 22 IB 22 2B 23 1A 23 2A 23 IB 23 2B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 - - - - - - - - - - - " 2 18 2 18 0 20 1 19 2 18 2 18 0 20 1 19 2 18 2 18 0 20 1 19 2 18 2 18 0 20 1 19 2 18 2 18 0 20 1 19 2 18 2 18 0 20 1 19 2 18 2 18 0 20 1 19 2 18 2 18 0 20 1 19 - - - - - - - - - - - - - _ - - Company Sanitized. Does not contain TSCA CBI -50- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate ^ Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 2 4 1A 0 0 24 2A 0 0 2 4 IB 0 0 24 2B 0 0 2 5 1A 00 25 2A 0 0 2 5 IB 0 0 25 2B 0 0 2 6 1A 0 0 26 2A 0 0 2 6 IB 0 0 26 2B 0 0 2 7 1A 0 0 27 2A 0 0 2 7 IB 1 0 27 2B 0 0 2 8 1A 0 0 28 2A 0 0 2 8 IB 0 0 28 2B 0 0 2 9 1A 0 0 29 2A 0 0 2 9 IB 0 0 29 2B 0 0 3 0 1A 0 0 30 2A 0 0 3 0 IB 0 0 30 2B 0 0 31 1A 0 0 31 2A 0 0 31 IB 0 0 31 2B 0 0 2 18 2 18 - 0 20 - 1 19 - 2 17 0 2 18 0 0 20 0 1 19 0 2 15 0 2 18 0 0 11 0 1 11 0 200 23 0 100 1 10 200 200 100 100 200 200 100 100 200 200 100 100 200 200 100 100 - - - 1 0 0 0 3 0 9 8 18 15 19 18 18 18 . 19 19 18 18 19 19 18 18 19 19 18 18 19 19 Company Sanitized. Does not contain TSCA CBS -51 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate 1 Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 3 2 1A 0 0 2 0 0 18 32 2A 0 0 2 0 0 18 32 IB 0 0 1 0 0 19 32 2B 0 0 1 0 0 19 33 1A 0 0 2 0 0 18 33 2A 0 0 2 0 0 18 33 IB 0 0 1 0 0 19 33 2B 0 0 1 0 0 19 3 4 1A 0 0 2 0 0 18 34 2A 0 0 2 0 0 18 3 4 IB 0 0 1 0 0 19 34 2B 0 0 1 0 0 19 35 1A 0 0 2 0 0 18 35 2A 0 0 2 0 0 18 35 IB 0 0 1 0 0 19 35 2B 0 0 1 0 0 19 3 6 1A 0 0 2 0 0 18 36 2A 0 0 2 0 0 18 3 6 IB 0 0 1 0 0 1 9 lb 36 2B 0 0 1 0 0 19 3 7 1A 0 0 2 0 0 18 37 2A 0 0 2 0 0 18 3 7 IB 0 0 1 0 0 1 9 lb 37 2B 0 0 1 0 0 19 3 8 1A 0 0 2 0 0 18 38 2A 0 0 2 0 0 18 3 8 IB 00 1 0 0 1 9 lb 38 2B 0 0 1 0 0 19 39 1A 0 0 2 0 0 18 39 2A 0 0 2 0 0 18 3 9 IB 0 0 1 0 0 1 9 lb 39 2B 0 0 1 0 0 19 lo e s not contain C om paq Sanitized. -52- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate+ Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 4 0 1A 0 0 2 0 0 18 40 2A 00 2 0 0 18 4 0 IB 00 1 0 0 1 9 lb 40 2B 00 1 0 0 19 41 1A 00 2 0 0 18 41 2A 00 2 0 0 18 41 IB 0 1 1 0 1 18 41 2B 0 0 1 0 0 19 4 2 1A 0 0 2 0 0 18 42 2A 00 2 0 0 18 4 2 IB 0 0 1 0 1 18 42 2B 0 0 1 0 0 19 4 3 1A 0 0 2 0 0 18 43 2A 0 0 2 0 0 18 4 3 IB 00 10 1 18 43 2B 0 0 1 0 0 19 44 1A 0 0 2 0 0 18 44 2A 00 2 0 0 18 . 4 4 IB 00 1 0 1 18 44 2B 00 1 0 0 19 4 5 1A 0 0 2 0 0 18 45 2A 0 0 2 0 0 18 4 5 IB 00 1 0 1 18 45 2B 00 1 0 0 19 t Four replicates per concentration (i.e., 1A - 2B). T Dash (-) denotes no data available, b Pale in color. Company Sanitized. Does not contain TSCA CBI -53- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 10 IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicatef Removed Removed Eggs Left Larvae Larvae 0 1A 0 0 2A 0 - 0 IB 0 - 0 2B 0 - 1 1A 0 - 1 2A 0 - 1 IB 0 - 1 2B 0 2 1A 0 - 2 2A 0 - 2 IB 0 - 2 2B 0 3 1A 0 - 3 2A 0 - 3 IB 0 - 3 2B 0 - 4 1A 0 - 4 2A 0 - 4 IB 0 _ 4 2B 0 - 5 1A 0 - 5 2A 0 - 5 IB 0 - 5 2B 0 - 6 1A 0 - 6 2A 0 - 6 IB 0 - 6 2B 0 - 7 1A 0 - 7 2A 0 - 7 IB 0 - 7 2B 0 " 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 - 0 20 0 20 0 20 0 20 0 20 0 20 _ 0 20 0 20 - 0 20 0 20 0 20 0 20 0 20 0 20 - - - - - - _ _ - - - - - - - - - - - - Company Sanitized. Does not contain TSCA CBf H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate f Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 8 1A 0 8 2A 0 - 8 IB 0 - 8 2B 0 - 9 1A 0 - 9 2A 0 9 IB 0 - 9 2B 0 - 10 1A 0 - 10 2A 1 - 10 IB 0 - 10 2 B 0 - 11 1A 0 - 11 2A 0 - 11 IB 0 - 11 2B 1 - 12 1A 0 - 12 2A 0 - 12 IB 0 - 12 2B 0 - 13 1A 2 - 13 2A 1- 13 IB 2 - 13 2B 1- 14 1A 0 - 14 2A 0 _ 14 IB 1 - 14 2B 2 - 15 1A 0 - 15 2A 0 - 15 IB 0 - 15 2B 0 " 0 20 0 20 - 0 20 - 0 20 - 0 20 0 20 - 0 20 0 20 0 20 1 19 0 20 0 20 - - - 0 20 - 1 19 - 0 20 - 1 19 - 0 20 - 1 19 0 20 - 1 19 2 18 2 18 2 18 2 18 2 18 2 18 3 17 4 16 - 2 18 2 18 3 17 - 4 16 ~ _ - - - - - - - - - - - - - - - - " pon.panyS0nmzed.Doa8 not contain TSCA CB1 -55- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate 1 Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 16 1A 0 16 2A 0 - 16 IB 0 - 16 2B 0 - 17 1A 0 - 17 2A 0 - 17 IB 0 - 17 2B 0 - 18 1A 0 - 18 2A 0 - 18 IB 0 - 18 2B 0 - 19 1A 0 - 19 2A 0 - 19 IB 0 - 19 2B 0 - 20 1A 0 - 20 2A 0 - 20 IB 0 - 20 2 B 0 - 21 1A 0 - 21 2A 0 - 21 IB 0 - 21 2 B 0 - 22 1A 0 - 22 2A 0 - 22 IB 0 - 22 2B 0 _ 23 1A 0 - 23 2A 0 - 23 IB 0 - 23 2B 0 - 2 18 2 18 - 3 17 - 4 16 - 0h0`-- 2- 2 18 . - 3 17 4 16 - 2 18 - 2 18 - 3 17 - 4 16 - 2 18 - 2 18 - 3 17 - 4 16 - 2 18 - 2 18 - 3 17 - 4 16 - 2 18 - 2 18 - 3 17 - 4 16 - 2 18 - 2 18 - 3 17 - 4 16 - 2 18 - 2 18 - 3 17 - 4 16 - - - - _ - ~ Company Sanitized. Does not contain TSCA CBl -56- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate : Removed Removed Eggs Left Larvae Larvae 2 4 1A 0 0 2 18 24 2A 0 0 2 18 - - 2 4 IB 0 0 3 17 - - 24 2B 0 0 4 16 - - 2 5 1A 0 0 2 18 0 0 25 2A 0 0 2 18 0 0 25 IB 0 0 3 16 0 1 25 2B 0 0 4 16 0 0 2 6 1A 0 0 2 12 0 6 26 2A 0 0 2 16 0 2 2 6 IB 0 0 3 13 0 4 2 6 2B 0 0 4 11 0 5 2 7 1A 0 0 2 0 0 18 27 2A 0 0 2 1 0 17 2 7 IB 0 0 3 0 0 17 27 2B 0 0 4 0 0 16 28 1A 0 0 20 0 18 28 2A 0 0 20 0 18 28 IB 0 0 3 0 0 17 28 2B 0 0 4 0 0 16 2 9 1A 0 0 2 0 0 18 29 2A 0 0 2 0 0 18 2 9 IB 0 0 3 0 0 17 29 2B 0 0 4 0 0 16 3 0 1A 0 0 2 0 0 18 30 2A 0 0 2 0 0 18 3 0 IB 0 0 3 0 0 17 30 2B 0 0 4 0 0 16 31 1A 0 0 2 0 0 18 31 2A 0 0 2 0 0 18 31 IB 0 0 3 0 0 17 31 2B 0 0 4 0 0 16 Company Sanitized. Does not contain TSCA CS -57- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate f Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 3 2 1A 0 0 2 0 0 18 32 2A 0 0 2 0 0 18 3 2 IB 00 3 0 0 17 32 2B 0 0 4 0 0 16 3 3 1A 00 2 0 0 18 33 2A 00 2 0 0 18 33 IB 0 0 3 0 0 17 33 2B 00 4 0 0 16 3 4 1A 00 2 0 0 18 34 2A 00 2 0 0 18 3 4 IB 00 3 0 0 17 34 2B 00 4 0 0 16 35 1A 0 0 2 0 0 18 35 2A 0 0 2 0 0 18 35 IB 00 3 0 0 17 35 2B 0 0 4 0 0 16 36 1A 0 0 2 0 0 18 36 2A 0 0 2 0 0 18 3 6 IB 00 3 0 0 17 36 2B 0 0 4 0 0 16 3 7 1A 00 2 0 0 18 37 2A 0 0 2 0 0 18 3 7 IB 00 3 0 0 17 37 2B 0 0 4 0 0 16 3 8 1A 00 2 0 0 18 38 2A 0 0 2 0 0 18 3 8 IB 00 3 0 0 17 38 2B 00 4 0 0 16 3 9 1A 0 0 2 0 0 18 39 2A 0 0 2 0 0 18 3 9 IB 00 3 0 0 17 39 2B 0 0 4 0 0 16 ^ c o n ta in T8CACB1 joes Sanl*edC*nPanY -58- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicatet Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 40 1A 0 0 40 2A 0 0 40 IB 0 0 40 2B 0 0 41 1A 0 0 41 2A 0 0 41 IB 0 0 41 2B 0 0 42 1A 0 0 42 2A 0 0 42 IB 0 0 42 2B 0 0 43 1A 0 0 43 2A 0 0 43 IB 0 0 43 2B 0 0 44 1A 0 0 44 2A 0 0 44 IB 0 0 44 2B 0 0 45 1A 0 0 45 2A 0 0 45 IB 0 0 45 2B 0 0 20 20 30 40 20 20 30 40 20 20 30 40 20 20 30 40 20 20 30 40 20 20 30 40 0 18 0 18 0 17 0 16 0 18 0 18 0 17 0 16 0 18 0 18 0 17 0 16 0 18 0 18 0 17 0 16 0 18 0 18 0 17 . 0 16 0 18 0 18 0 17 0 16 t Four replicates per concentration (i.e., 1A-2B). T Dash (-) denotes no data available. ,, ,, ,, ,, sanw zed. Doe* no. contain TSOA CB> -59- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 11 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate ' Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 0 1A 0 0 2A 0 - 0 IB 0 - 0 2B 0 - 1 1A 0 - 1 2A 0 - 1 IB 0 - 1 2B 0 - 2 1A 0 - 2 2A 0 - 2 IB 0 - 2 2B 0 - 3 1A 0 - 3 2A 0 - 3 IB 0 - 3 2B 0 - 4 1A 0 - 4 2A 0 - 4 IB 0 - 4 2B 0 - 5 1A 0 - 5 2A 0 - 5 IB 0 _ 5 2B 0 - 6 1A 0 - 6 2A 0 - 6 IB 0 - 6 2B 0 - 7 1A 1 - 7 2A 1- 7 IB 0 - 7 2B 0 - 0 20 0 20 - 0 20 - 0 20 - 0 20 _ 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 1 19 - 1 19 - 0 20 - 0 20 ~ " Company Sanitized. Does not contain TSCA CBl -60- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 11 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate ^ Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 8 1A 0 8 2A 0 - 8 IB 0 - 8 2B 0 - 9 1A 0 - 9 2A 0 - 9 IB 0 - 9 2B 0 - 10 1A 0 - 10 2A 0 - 10 IB 0 - 10 2B 0 - 11 1A 0 - 11 2 A 0 - 11 IB 0 - 11 2B 0 - 12 1A 1 _ 12 2 A 2 - 12 IB 1 - 12 2 B 0 - 13 1A 2 - 13 2A 2 - 13 IB 1 - 13 2B 3 - 14 1A 0 - 14 2A 0 - 14 IB 0 - 14 2B 0 - 15 1A 0 - 15 2A 0 - 15 IB 0 - 15 2B 0 - 1 19 1 19 0 20 0 20 1 19 1 19 0 20 0 20 1 19 1 19 0 20 0 20 1 19 1 19 0 20 0 20 2 18 ---------- ------- 3 17 1 19 0 20 - - 4 16 - - 5 15 2 18 - - 3 17 - - 4 16 - - 5 15 2 18 - - 3 17 - - 4 16 - - 5 15 - 2 18 - - 3 17 - - -61 - company S a n t o d . Doaa nol contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 11 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate+ Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 16 1A 0 16 2A 0 - 16 IB 0 - 16 2B 0 - 17 1A 0 - 17 2A 0 - 17 IB 0 - 17 2B 0 - 18 1A 0 - 18 2A 0 - 18 IB 0 - 18 2B 0 - 19 1A 0 - 19 2A 0 - 19 IB 0 - 19 2B 0 - 20 1A 0 - 20 2A 0 - 20 IB 0 - 20 2B 0 - 21 1A 0 - 21 2A 0 - 21 IB 0 - 21 2B 0 - 22 1A 0 - 22 2A 0 - 22 IB 0 - 22 2B 0 - 2 3 1A 0 - 2 3 2A 0 - 2 3 IB 0 - 2 3 2B 0 - 4 16 5 15 - - 2 18 - 3 17 - - 4 16 - - 5 15 - - 2 18 - - 3 17 - - 4 16 - - 5 15 - - 2 18 - - - 3 17 - - 4 16 - - 5 15 - - 2 18 - - 3 17 - - 4 16 - - 5 15 - - 2 18 3 17 - " - 4 16 - - 5 15 - - 2 18 - - 3 17 - - 4 16 - - 5 15 - - 2 18 - - 3 17 - - 4 16 - - 5 15 - 2 18 - - 3 17 - " SCACi^ Sanitfced-ooes notconiain * -62- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 11 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate f Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 24 1A 0 24 2A 0 - 24 IB 0 - 24 2B 0 - 25 1A 0 0 25 2A 0 0 25 IB 0 0 25 2B 0 0 26 1A 0 0 26 2A 0 0 26 IB 0 0 26 2 B 0 0 27 1A 0 0 27 2A 0 0 27 IB 0 0 27 2B 0 0 28 1A 0 0 28 2A 0 0 28 IB 0 0 28 2 B 0 0 29 1A 0 0 29 2A 0 0 29 IB 0 0 29 2B 0 0 30 1A 0 0 30 2A 0 0 30 IB 0 0 30 2 B 0 0 31 1A 0 0 31 2 A 0 0 31 IB 0 0 31 2B 0 0 4 16 5 15 2 18 3 17 4 16 0 5 15 0 2 18 0 3 16 0 4 11 0 550 2 12 0 3 10 0 420 500 220 3 10 400 500 200 300 400 500 200 300 4 00 500 200 300 400 500 200 300 - - - 0 0 0 1 5 10 6 7 14 15 16 16 16 15 18 . 17 16 15 18 17 16 15 18 17 16 15 18 17 C om paq Sanitized. Doe. not contain TSCA H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 11 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate ' Removed Removed Eggs Left Larvae Larvae 32 1A 0 0 4 0 0 16 32 2A 0 0 5 0 0 15 32 IB 0 0 2 0 0 18 32 2B 0 0 3 0 0 17 33 1A 0 0 4 0 0 16 33 2A 0 0 5 0 0 15 33 IB 0 0 2 0 0 18 33 2B 0 0 3 0 0 17 34 1A 0 0 4 0 0 16 34 2A 0 0 5 0 0 15 34 IB 0 0 2 0 0 18 34 2B 0 0 3 0 0 17 35 1A 0 0 4 0 0 16 35 2A 0 0 5 0 0 15 35 IB 0 0 2 0 0 18 35 2B 0 0 3 0 0 17 36 1A 0 0 4 0 0 16 36 2A 0 0 5 0 0 15 36 IB 0 0 2 0 0 IB 36 2B 0 0 3 0 0 17 37 1A 0 0 4 0 0 16 37 2A 0 0 5 0 0 15 37 IB 0 0 2 0 0 18 37 2B 0 0 3 0 0 17 38 1A 0 0 4 0 0 16 38 2A 0 0 5 0 0 15 38 IB 0 0 2 0 0 18 38 2B 0 0 3 0 0 17 39 1A 0 0 4 0 0 16 39 2A 0 0 5 0 0 15 39 IB 0 0 2 0 0 18 39 2B 0 0 3 0 0 17 C o m p a ra m iSxeti Does not contaisinT -64- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 11 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicatef Removed Removed Eggs Left Larvae Larvae 40 1A 0 0 4 0 0 16 40 2A 0 0 5 0 0 15 40 IB 0 0 2 0 0 18 40 2B 0 0 3 0 0 17 41 1A 0 0 4 0 0 16 41 2A 0 0 5 0 0 15 41 IB 0 0 2 0 0 18 41 2B 0 0 3 0 0 17 42 1A 0 0 4 0 0 16 42 2A 0 0 5 0 0 15 42 IB 0 0 2 0 0 18 42 2B 0 0 30 0 17 43 1A 0 0 4 0 0 16 43 2A 0 0 50 0 15 43 IB 0 0 2 0 0 18 43 2B 0 0 30 0 17 44 1A 0 0 4 0 0 16 44 2A 0 0 5 0 0 15 44 IB 0 0 2 0 0 18 44 2B 0 0 3 0 0 17 45 1A 0 0 4 0 0 16 45 2A 0 0 5 0 0 15 45 IB 0 0 2 0 0 18 45 2B 0 0 3 0 0 17 t Four replicates per concentration (i.e., 1A - 2B). T Dash (-) denotes no data available. Sanitized. Does not contain TSCACBl co m p ly H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 12 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate f Removed Removed Eggs Left Larvae Larvae 0 1A 0 0 2A 0 - 0 IB 0 - 0 2B 0 - 1 1A 0 - 1 2A 0 - 1 IB 0 - 1 2B 0 - 2 1A 0 - 2 2A 0 - 2 IB 0 - 2 2B 0 - 3 1A 0 - 3 2A 0 - 3 IB 0 - 3 2B 0 - 4 1A 0 - 4 2A 0 - 4 IB 0 - 4 2B 0 - 5 1A 0 - 5 2A 0 - 5 IB 0 - 5 2B 0 - 6 1A 0 - 6 2A 0 - 6 IB 0 - 6 2B 0 - 7 1A 0 - 7 2A 0 - 7 IB 0 - 7 2B 0 - 0 20 0 20 - 0 20 0 20 - 0 20 0 20 0 20 - 0 20 0 20 0 20 0 20 - 0 20 0 20 0 20 0 20 - 0 20 0 20 - 0 20 - 0 20 0 20 0 20 - 0 20 0 20 - 0 20 - 0 20 0 20 0 20 0 20 - 0 20 - 0 20 -a 0 20 0 20 - - - - - - - - - - -a - - not contain TSCAC* Company Sanitized. D o o $ -66- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 12 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicatet Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 8 1A 0 8 2A 1 - 8 IB 0 - 8 2B 0 - 9 1A 0 - 9 2A 0 - 9 IB 0 - 9 2B 0 - 10 1A 1 - 10 2A 0 - 10 IB 1 _ 10 2B 0 - 11 1A 0 - 11 2A 0 - 11 IB 0 - 11 2B 0 - 12 1A 0 _ 12 2A 0 - 12 IB 0 - 12 2B 0 - 13 1A 2 - 13 2A 1 - 13 IB 2 - 13 2B 2 - 14 1A 0 - 14 2A 0 - 14 IB 0 - 14 2B 0 - 15 1A 0 - 15 2A 0 - 15 IB 0 - 15 2B 0 0 20 1 19 0 20 0 20 0 20 1 19 0 20 0 20 1 19 1 19 1 19 0 20 1 19 1 19 1 19 0 20 1 19 1 19 1 19 0 20 3 17 2 18 3 17 2 18 3 17 2 18 3 17 2 18 3 17 2 18 3 17 2 18 - - - - - - - - _ - - - - - - - - - - - - - - - - - - - - - - _ - - - r Sanitized. Does not contain Compaq -67- H -24678: Early Life-Stage Toxicity to Rainbow Trout, O ncorhynchus m ykiss DuPont-11603 TABLE 12 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicatet Removed Removed Eggs Left Larvae Larvae 16 1A 0 16 2A 0 16 IB 0 - 16 2B 0 17 1A 0 - 17 2A 0 - 17 IB 0 - 17 2B 0 - 18 1A 0 - 18 2A 0 - 18 IB 0 - 18 2B 0 - 19 1A 0 19 2A 0 - 19 IB 0 - 19 2B 0 - 20 1A 0 - 20 2A 0 - 20 IB 0 - 20 2B 0 - 21 1A 0 - 21 2A 0 - 21 IB 0 - 21 2B 0 - 22 1A 0 - 22 2A 0 - 22 IB 0 - 22 2B 0 - 23 1A 0 - 23 2A 0 - 23 IB 0 - 23 2B 0 3 17 2 18 3 17 2 18 3 17 2 18 3 17 2 18 3 17 2 18 3 17 2 18 3 17 2 18 - 3 17 - 2 18 3 17 2 18 - 3 17 2 18 - 3 17 _ 2 18 - 3 17 2 18 3 17 2 18 3 17 2 18 3 17 - 2 18 3 17 2 18 - - - - - - - - - - - _ - - - - - - not contain TSCACB1 Go'Pp'*/ Sartiiszed. Does -68- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Ortcorhynchus m ykiss DuPont-11603 TABLE 12 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate ^ Removed Removed Eggs Left Larvae Larvae 24 1A 0 3 17 24 2A 0 - 2 18 - - 24 IB 0 - 3 17 - - 24 2B 0 - 2 18 - - 25 1A 0 0 3 17 0 0 25 2A 0 0 2 17 0 j a,d 25 IB 0 0 3 16 0 ^a,d 25 2B 0 0 2 17 0 l 26 1A 0 0 3 9 0 8 26 2A 0 0 2 9 0 gUA 26 IB 0 0 3 11 0 6Ia'd 26 2B 0 0 2 8 0 10 27 1A 0 0 3 1 0 16 27 2A 0 0 2 0 0 18la,d 27 IB 0 0 3 0 0 y la,d 27 2B 0 0 2 0 0 18 28 1A 0 0 3 1 0 16 28 2A 0 0 2 0 0 18la,d 28 IB 0 0 3 0 0 j y la,d 28 2B 0 0 2 0 0 18 29 1A 0 0 3 0 0 17 29 2A 0 0 2 0 0 18la,d 29 IB 0 0 3 0 0 jy l a ,d 29 2B 0 0 2 0 0 18 30 1A 0 0 3 0 0 17 30 2A 0 0 2 0 0 18la,d 30 IB 0 0 3 0 0 ^ y la,d 30 2B 0 0 2 0 0 18 31 1A 0 0 3 0 0 j y lb,d 31 2A 0 0 2 0 0 18Ia,d 31 IB 0 1 30 1 16 31 2B 0 0 2 0 0 18 -69- Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 12 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate + Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 32 1A 0 0 3 0 0 Jrjlb,d 32 2A 0 0 2 0 0 18`a.d 32 IB 0 0 3 0 1 16 32 2B 0 0 2 0 0 18 33 1A 0 0 3 0 0 j f-jlbtd 33 2A 0 0 2 0 0 jgla.b.d 33 IB 0 0 3 0 1 16 33 2B 0 0 2 0 0 18 34 1A 0 0 3 0 0 j-ylb,d 34 2A 0 0 2 0 0 |gla,b,d 34 IB 0 0 3 0 1 16 34 2B 0 0 2 0 0 18 35 1A 0 0 3 0 0 j-yIb,d 35 2A 0 0 2 0 0 ^gla,b,d 35 IB 0 0 30 1 16 35 2B 0 0 2 0 0 18 36 1A 0 0 3 0 0 I7 IM 36 2A 0 0 2 0 0 j gla,b,d 36 IB 0 0 30 1 16 36 2B 0 0 2 0 0 18 37 1A 0 0 30 0 jylb.d 37 2A 0 0 2 0 0 jglaTb,d 37 IB 0 0 30 1 16 37 2B 0 0 2 0 0 18 38 1A 0 0 3 0 0 27),d 38 2A 0 1 20 1 17 38 IB 0 0 30 1 16 38 2B 0 0 20 0 18 39 1A 0 0 3 0 0 j 7 lb,d 39 2A 0 0 20 1 17 39 IB 0 0 30 1 16 39 2B 0 0 2 0 0 18 Sanitized. Does not contain TSCA CBi pompany -70- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 12 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate ^ Removed Removed Eggs Left Larvae Larvae 40 1A 00 3 0 0 40 2 A 00 2 0 1 17 40 IB 00 3 0 1 16 40 2 B 00 2 0 0 18 41 1A 00 30 0 j^ylb,d 41 2 A 00 2 0 1 17 41 IB 00 3 0 1 16 41 2 B 00 2 0 0 18 42 1A 0 1 3 0 1 16 42 2 A 00 2 0 1 17 42 IB 00 3 0 1 16 42 2 B 00 2 0 0 18 43 1A 00 30 1 16 43 2 A 00 2 0 1 17 43 IB 00 30 1 16 43 2 B 00 2 0 0 18 44 1A 00 3 0 1 16 44 2 A 00 2 0 1 17 44 IB 00 3 0 1 16 44 2 B 00 2 0 0 18 45 1A 00 3 0 1 16 45 2 A 00 2 0 1 17 45 IB 00 3 0 1 16 45 2 B 00 2 0 0 18 f Four replicates per concentration (i.e., 1A - 2B). T* Dash (-) denotes no data available, a Scoliosis b Pale in color d Small in size Company Sanitized. Does not contain TSCA CB1 -71 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 13 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate 1' Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 0 1A 0 0 2A 0 - 0 IB 0 - 0 2B 0 - 1 1A 0 - 1 2A 0 - 1 IB 0 - 1 2B 0 - 2 1A 0 - 2 2A 0 - 2 IB 0 - 2 2B 0 - 3 1A 0 - 3 2A 0 - 3 IB 0 - 3 2B 0 - 4 1A 0 - 4 2A 0 - 4 IB 0 - 4 2B 0 - 5 1A 0 - 5 2A 0 - 5 IB 0 - 5 2B 0 - 6 1A 0 - 6 2A 0 - 6 IB 0 - 6 2B 0 - 7 1A 0 - 7 2A 0 - 7 IB 0 - 7 2B 0 - 0 20 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - _ _ - goinpany Sanitized- Does c o fi^ n TSCA C8, -72- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate * Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 8 1A 0 8 2A 0 8 IB 0 - 8 2B 0 - 9 1A 0 - 9 2A 0 - 9 IB 0 - 9 2B 1 - 10 1A 0 - 10 2A 0 - 10 IB 0 - 10 2B 0 - 11 1A 0 - 11 2A 0 - 11 IB 0 - 11 2B 0 - 12 1A 0 - 12 2A 0 - 12 IB 0 - 12 2B 0 - 13 1A 3 - 13 2A 1 - 13 IB 1 - 13 2B 1 - 14 1A 0 _ 14 2A 0 - 14 IB 0 - 14 2B 0 - 15 1A 0 - 15 2A 0 - 15 IB 0 - 15 2B 0 - 0 20 0 20 0 20 0 20 0 20 0 20 0 20 1 19 0 20 0 20 0 20 1 19 0 20 0 20 0 20 1 19 0 20 0 20 0 20 1 19 3 17 1 19 1 19 2 18 3 17 1 19 1 19 2 18 3 17 1 19 1 19 2 18 _ - - " Company Sanitized. Does not contain TSCA CB1 -73 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate f Removed Removed Eggs Left Larvae Larvae 16 1A 0 16 2A 0 - 16 IB 0 - 16 2B 0 17 1A 0 - 17 2A 0 - 17 IB 0 17 2B 0 - 18 1A 0 - 18 2A 0 18 IB 0 - 18 2B 0 - 19 1A 0 19 2A 0 - 19 IB 0 - 19 2B 0 - 20 1A 0 - 20 2A 0 - 20 IB 0 - 20 2B 0 - 21 1A 0 - 21 2A 0 - 21 IB 0 - 21 2B 0 - 22 1A 0 - 22 2A 0 - 22 IB 0 - 22 2B 0 - 23 1A 0 - 23 2A 0 - 23 IB 0 - 23 2B 0 - 3 17 1 19 - 1 19 2 18 _ 3 17 1 19 1 19 2 18 3 17 1 19 1 19 2 18 3 17 1 19 - 1 19 2 18 3 17 1 19 - 1 19 2 18 - 3 17 - 1 19 1 19 - 2 18 - 3 17 - 1 19 1 19 - 2 18 - 3 17 - 1 19 1 19 2 18 - - - - - - - - - - - - - - - _ - - - - not contain TSCA Cl C o,P8 * * i D o " -74- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicatef Removed Removed Eggs Left Larvae Larvae 24 1A 0 24 2A 0 - 24 IB 0 24 2B 0 - 25 1A 0 0 25 2A 0 0 25 IB 0 0 25 2B 0 0 26 1A 0 0 26 2A 0 0 26 IB 0 0 26 2B 0 0 27 1A 0 0 27 2A 0 0 27 IB 0 0 27 2B 0 0 28 1A 0 0 28 2A 0 0 28 IB 0 0 28 2B 10 29 1A 0 0 29 2A 0 0 29 IB 0 0 29 2B 0 0 30 1A 0 0 30 2A 0 0 30 IB 0 0 30 2B 0 0 31 1A 0 0 31 2A 0 0 31 IB 0 0 31 2B 0 0 3 17 1 19 - - 1 19 - - 2 18 - - 3 17 0 0 1 19 0 0 1 19 0 0 2 16 0 2 3 12 0 5 1 18 0 1 1 13 0 6 290 9 3 1 0 16 1 1 0 18 1 1 0 18 2 2 0 16 3 1 0 16 1 0 0 19 1 0 0 19 3 0 0 17 3 0 0 17 1 0 0 19 1 0 0 19 3 0 0 17 3 0 0 17 1 0 0 19 1 0 0 19 3 0 0 17 3 0 0 17 1 0 0 19 1 0 0 19 3 0 0 17 C o m p ly Sanitized. Does not contain TSCA CB1 -75- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate * Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 32 1A 0 0 3 0 0 17 32 2A 00 1 0 0 19 32 IB 00 1 0 0 19 32 2B 00 3 0 0 17 33 1A 00 3 0 0 17 33 2A 00 1 0 0 19 33 IB 00 1 0 0 19 33 2B 0 0 3 0 0 17 34 1A 0 0 3 0 0 17 34 2A 0 0 1 0 0 19 34 IB 00 1 0 0 19 34 2B 0 0 3 0 0 17 35 1A 0 0 3 0 0 17 35 2A 0 0 1 0 0 19 35 IB 0 0 1 0 0 19 35 2B 00 3 0 0 17 36 1A 0 0 3 0 0 17 36 2A 0 0 1 0 0 19 36 IB 0 0 1 0 0 19 36 2B 0 0 3 0 0 17 37 1A 0 0 3 0 0 17 37 2A 0 0 1 0 0 19 37 IB 00 1 0 0 19 37 2B 0 0 3 0 0 17 38 1A 00 3 0 0 17 38 2A 0 0 1 0 0 19 38 IB 00 1 0 0 19 38 2B 00 3 0 0 17 39 1A 00 3 0 0 17 39 2A 00 1 0 0 19 39 IB 00 1 0 0 19 39 2B 0 0 3 0 0 17 Company Sanitized. Does not contain TSCA CSI -76- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate ' Removed Removed Eggs Left Larvae Larvae 40 1A 0 0 3 0 0 17 40 2A 0 0 10 0 19 40 IB 0 0 10 0 19 40 2B 0 0 3 0 0 17 41 1A 0 0 3 0 0 17 41 2A 0 0 10 0 19 41 IB 0 0 10 0 19 41 2B 0 0 3 0 0 17 42 1A 0 0 3 0 0 17 42 2A 0 0 10 0 19 42 IB 0 0 10 0 19 42 2B 0 0 3 0 0 17 43 1A 0 0 3 0 0 17 43 2A 0 0 10 0 19 43 IB 0 0 10 0 19 43 2B 0 0 3 0 0 17 44 1A 0 0 3 0 0 17 44 2A 0 0 10 0 19 44 IB 0 0 10 0 19 44 2B 0 0 3 0 0 17 45 1A 0 0 3 0 0 17 45 2A 0 0 10 0 19 45 IB 0 0 10 0 19 45 2B 0 0 3 0 0 17 f Four replicates per concentration (i.e., 1A - 2B). Dash (-) denotes no data available. Company Sanitized. Does not contain TSGA C B t -77 H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 14 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicatet Removed Removed Eggs Left Larvae Larvae 0 1A 0 0 2A 0 - 0 IB 0 - 0 2B 0 - 1 1A 0 1 2A 0 - 1 IB 0 - 1 2B 0 2 1A 0 _ 2 2A 0 - 2 IB 0 - 2 2B 0 - 3 1A 3 2A 0 0 - _ 3 IB 0 - 3 2B 0 - 4 1A 0 - 4 2A 0 - 4 IB 0 - 4 2B 0 - 5 1A 3 - 5 2A 2 - 5 IB 1- 5 2B 1 - 6 1A 1 - 6 2A 1 - 6 IB 1- 6 2B 1 - 7 1A 0 - 7 2A 0 - 7 IB 0 - 7 2B 0 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 0 20 3 17 2 18 1 19 1 19 4 16 3 17 2 18 2 18 4 16 3 17 2 18 2 18 - - - - - - - - - - - - _ - - - _ - - - - - - - - _ - - - - - - - - - - - com pany Sanitized. Does not contain TSCft CBI -78- H -24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 14 (Continued) 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicatet Removed Removed Eggs Left Larvae Larvae 8 1A 0 8 2A 0 8 IB 0 - 8 2B 0 9 1A 1 - 9 2A 1- 9 IB 1- 9 2B 1 - 10 1A 0 - 10 2A 0 - 10 IB 0 10 2B 0 - 11 1A 0 - 11 2A 0 - 11 IB 0 - 11 2B 0 - 12 1A 0 - 12 2A 3 - 12 IB 2 - 12 2B 3 - 13 1A 0 - 13 2A 1- 13 IB 0 - 13 2B 0 - 14 1A 0 - 14 2A 0 - 14 IB 0 - 14 2B 0 - 15 1A 0 - 15 2A 0 - 15 IB 0 - 15 2B 0 " 4 16 3 17 2 18 2 18 5 15 4 16 - 3 17 3 17 5 15 4 16 - 3 17 3 17 5 15 4 16 - 3 17 - 3 17 - 5 15 - 7 13 - 5 15 - 6 14 - 5 15 - 8 12 - 5 15 - 6 14 - 5 15 - 8 12 - 5 15 _ 6 14 _ 5 15 - 8 12 - 5 15 6 14 - - - - - _ - - - - - - - - - - - - - -79- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 14 (Continued) 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate f Removed Removed Eggs Left Larvae Larvae 16 1A 0 16 2A 0 - 16 IB 0 - 16 2B 0 - 17 1A 0 - 17 2A 0 - 17 IB 0 - 17 2B 0 - 18 1A 0 - 18 2A 0 - 18 IB 0 - 18 !,v 2B 0 - 19 1A 0 - 19 2A 0 - 19 IB 0 _ 19 2B 0 - 20 1A 0 - 20 2A 0 - 20 IB 0 20 2B 0 - 21 1A 0 - 21 2A 0 - 21 IB 0 - 21 2B 0 - 22 1A 0 - 22 2A 0 - 22 IB 0 - 22 2B 0 - 23 1A 0 - 23 2A 0 - 23 IB 0 - 23 2B 0 5 15 8 12 5 15 6 14 5 15 8 12 5 15 6 14 5 15 8 12 5 15 6 14 5 15 8 12 5 15 6 14 5 15 8 12 5 15 6 14 5 15 8 12 5 15 6 14 5 15 8 12 5 15 6 14 5 15 8 12 5 15 6 14 - _ _ - - COW *., Sani *. *" C" -80- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 14 (Continued) 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate t Removed Removed Eggs Left Larvae Larvae 24 1A 0 24 2A 0 0 24 IB 0 - 24 2B 0 - 25 1A 0 0 25 2A 0 0 25 IB 0 0 25 2B 0 0 26 1A 0 0 26 2A 0 0 26 IB 0 0 26 2B 0 0 27 1A 0 0 27 2A 0 0 27 IB 0 0 27 2B 0 0 28 1A 0 0 28 2A 0 0 28 IB 0 0 28 2B 00 29 1A 1 0 29 2A 0 0 29 IB 00 29 2B 0 0 30 1A 0 0 30 2A 0 0 30 IB 0 0 30 2B 0 0 31 1A 0 0 31 2A 0 0 31 IB 0 0 31 2B 0 0 5 15 8 11 0 5 15 6 14 _ 5 15 0 8 10 0 5 14 0 6 14 0 590 82 0 5 80 6 12 0 520 80 0 500 620 5 10 80 0 500 600 600 80 0 500 600 600 80 0 5 00 600 600 80 0 500 600 1 - _ 0 2 1 0 6 10 7 2 13 12 15 12 14 12 15 14 14 12 15 14 14 12 15 14 14 12 15 14 sanitized. Does net contain TSCACBI Company -81 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 14 (Continued) 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Day Replicatet Removed Removed Dead Eggs Eggs Dead Live Left Larvae Larvae 32 1A 0 0 6 0 0 14 32 2A 0 0 8 0 0 12 32 IB 0 0 50 0 15 32 2B 0 0 6 0 0 14 33 1A 0 0 6 0 0 14 33 2A 0 0 8 0 0 12 33 IB 0 0 5 0 0 15 33 2B 0 0 60 0 14 34 1A 0 0 6 0 0 14 34 2A 0 0 8 0 0 12 34 IB 0 0 5 0 0 15 34 2B 0 0 6 0 0 14 35 1A 0 0 60 0 14 35 2A 0 0 80 0 12 35 IB 0 0 5. 0 0 15 35 2B 0 0 6 0 0 14 36 1A 0 0 60 0 14 36 2A 0 0 80 0 12 36 IB 0 0 50 0 15 36 2B 0 0 6 0 0 14 37 1A 0 0 6 0 0 14 37 2A 0 0 8 0 0 12 37 IB 0 0 50 0 15 37 2B 0 0 6 0 0 14 38 1A 0 0 6 0 0 14 38 2A 0 0 80 0 12 38 IB 0 0 5 0 0 15 38 2B 0 0 6 0 0 14 39 1A 0 0 6 0 0 14 39 2A 0 0 8 0 0 12 39 IB 0 0 5 0 0 15 39 2B 0 0 6 0 0 14 Company Sanitized. Does not contain TSCA CBI -82- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 14 (Continued) 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate ` Removed Removed Eggs Left Larvae Larvae 40 1A 0 0 6 0 0 14 40 2A 0 0 8 0 0 12 40 IB 0 0 50 0 15 40 2B 0 0 6 0 0 14 41 1A 0 0 6 0 0 14 41 2A 0 0 8 0 0 12 41 IB 0 0 5 0 0 15 41 2B 0 0 6 0 0 14 42 1A 0 0 6 0 0 14 42 2A 0 0 8 0 0 12 42 IB 0 0 5 0 0 15 42 2B 0 0 6 0 0 14 43 1A 0 0 6 0 0 14 43 2A 0 0 8 0 0 12 43 IB 0 1 5 0 1 14 43 2B 0 0 6 0 0 14 44 1A 0 0 6 0 0 14 44 2A 0 0 8 0 0 12 44 IB 0 0 5 0 1 14 44 2B 0 0 6 0 0 14 45 1A 0 0 6 0 0 14 45 2A 0 0 8 0 0 12 45 IB 0 0 5 0 1 14 45 2B 0 0 6 0 0 14 t Four replicates per concentration (i.e., 1A - 2B). Dash (-) denotes no data available. Comp" '>'s f e Boranot contain TSCACBI -83- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 15 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicatet Removed Removed Eggs Left Larvae Larvae 0 1A 0 0 2A 0 - 0 IB 0 - 0 2B 0 - 1 1A 0 - 1 2A 0 - 1 IB 0 - 1 2B 0 2 1A 0 - 2 2A 0 - 2 IB 0 - 2 2B 0 - 3 1A 0 - 3 2A 0 - 3 IB 0 - 3 2B 0 - 4 1A 0 - 4 2A 0 _ 4 IB 0 - 4 2B 0 - 5 1A 0 - 5 2A 0 - 5 IB 2 - 5 2B 4 - 6 1A 0 - 6 2A 0 - 6 IB 0 - 6 2B 0 - 7 1A 0 - 7 2A 0 _ 7 IB 0 - 7 2B 0 - 0 20 0 20 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 0 20 - 2 18 - 4 16 - 0 20 - 0 20 - 2 18 - 4 16 - 0 20 _ 0 20 - 2 18 - 4 16 - - - - - - _ _ _ _ - Company Sanitized. Does not contain TSGA C8 -84- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate t Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 8 1A 0 8 2A 0 - 8 IB 0 - 8 2B 0 - 9 1A 0 - 9 2A 0 - 9 IB 1 - 9 2B 0 - 10 1A 0 - 10 2A 0 - 10 IB 0 - 10 2B 0 - 11 1A 0 - 11 2A 0 - 11 IB 0 - 11 2B 0 - 12 1A 2 - 12 2A 5 - 12 IB 2 - 12 2B 2 - 13 1A 0 - 13 2A 0 - 13 IB 0 - 13 2B 0 - 14 1A 0 - 14 2A 0 - 14 IB 0 - 14 2B 0 - 15 1A 1 - 15 2A 0 - 15 IB 0 - 15 2B 0 0 20 0 20 - 2 18 - 4 16 - 0 20 0 20 3 17 _ 4 16 - 0 20 0 20 3 17 4 16 - 0 20 0 20 - 3 17 4 16 - 2 18 - 5 15 5 15 - 6 14 2 18 - 5 15 5 15 - 6 14 2 18 - 5 15 - 5 15 - 6 14 - 3 17 5 15 5 15 - 6 14 - - - - - - - - - " C ow an, tscacbi -85- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate * Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 16 1A 16 2A 16 IB 16 2B 17 1A 17 2A 17 IB 17 2B 18 1A 18 2A 18 IB 18 2B 19 1A 19 2A 19 IB 19 2B 20 1A 20 2A 20 IB 20 2B 21 1A 21 2A 21 IB 21 2B 22 1A 22 2A 22 IB 22 2B 23 1A 23 2A 23 IB 23 2B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 _ - " 3 17 5 15 _ 5 15 6 14 3 17 5 15 5 15 6 14 3 17 5 15 5 15 6 14 3 17 5 15 5 15 6 14 3 17 5 15 5 15 6 14 3 17 5 15 5 15 6 14 3 17 - 5 15 5 15 6 14 3 17 5 15 5 15 6 14 - _ _ - - Company Sanitized. Does not contain TSCA O il -86- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate * Removed Removed Eggs Left Larvae Larvae 24 1A 0 24 2A 0 - 24 IB 0 - 24 2B 0 - 25 1A 0 0 25 2A 0 0 25 IB 0 0 25 2B 0 0 26 1A 0 0 26 2A 0 0 26 IB 0 0 26 2B 0 0 27 1A 0 0 27 2A 0 0 27 IB 0 0 27 2B 0 0 28 1A 0 0 28 2A 0 0 28 IB 10 28 2B 0 0 29 1A 10 29 2A 0 0 29 IB 0 0 29 2B 0 0 30 1A 0 0 30 2A 0 0 30 IB 0 0 30 2B 0 0 31 1A 0 1 31 2A 0 0 31 IB 00 31 2B 0 0 3 17 5 15 5 15 6 14 3 16 0 5 15 0 5 14 0 6 13 0 370 54 0 590 680 3 10 500 5 10 600 3 10 500 600 600 400 500 600 600 400 500 500 600 401 500 600 600 - - - 1 0 1 la 10 11 6 6 16 15 14 14 16 15 14 . 14 16 15 14 14 16 15 14 14 15 15 14 14 Company Sanitized. Does not contain TSCA 68* -87- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Eggs Larvae Dead Day Replicate ' Removed Removed Eggs Live Eggs Left Cumulative Dead Larvae Live Larvae 32 1A 0 0 4 0 1 15 32 2A 0 0 5 0 0 15 32 IB 0 0 6 0 0 14 32 2B 0 0 6 0 0 j 4 la,b,d 33 1A 0 0 4 0 1 15 33 2A 0 0 5 0 0 15 33 IB 0 0 6 0 0 14 33 2B 0 0 6 0 0 14la,b,d 34 1A 0 0 4 0 1 15 34 2A 0 0 5 0 0 15 34 IB 0 0 34 2B 0 0 6 6 0 0 0 14 0 24la,b,d 35 1A 0 0 4 0 1 15 35 2A 0 0 5 0 0 15 35 IB 0 0 35 2B 0 0 6 6 0 0 0 14 0 2^1a,b,d 36 1A 0 0 4 0 1 15 36 2A 0 0 5 0 0 15 36 IB 0 0 6 0 0 14 36 2B 0 0 6 0 0 j^la,b,d 37 1A 0 0 4 0 1 15 37 2A 0 0 5 0 0 15 37 IB 0 0 6 0 0 14 37 2B 0 0 6 0 0 14'a.b.d 38 1A 0 0 4 0 1 15 38 2A 0 0 5 0 0 15 38 IB 0 0 6 0 0 14 38 2B 0 0 6 0 0 -j^la,b,d 39 1A 0 0 4 0 1 15 39 2A 0 0 5 0 0 15 39 IB 0 0 6 0 0 14 39 2B 0 0 6 0 0 14la,b,d Company Sanitized. Does notcontain TSCCil H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate t Removed Removed Eggs Left Larvae Larvae 40 1A 0 0 4 0 1 15 40 2A 0 0 5 0 0 15 40 IB 0 0 6 0 0 14 40 2B 0 1 6 0 1 13 41 1A 0 0 4 0 1 15 41 2A 0 0 5 0 0 15 41 IB 0 0 6 0 0 14 41 2B 0 0 6 0 1 13 42 1A 0 0 4 0 1 15 42 2A 0 0 5 0 0 15 42 IB 0 0 6 0 0 14 42 2B 0 0 6 0 1 13 43 1A 0 0 4 0 1 15 43 2A 0 0 5 0 0 15 43 IB 0 0 6 0 0 14 43 2B 0 0 6 0 1 13 44 1A 0 0 4 0 1 15 44 2A 0 0 5 0 0 15 44 IB 0 0 6 0 0 14 44 2B 0 0 6 0 1 13 45 1A 0 0 4 0 1 15 45 2A 0 0 5 0 0 15 45 IB 0 0 6 0 0 14 45 2B 0 0 6 0 1 13 t Four replicates per concentration (i.e., TA - 2B). T Dash (-) denotes no data available, a Scoliosis b Pale in color d Small in size Company Sanitized. Does net eonlafft TSGA CB1 -89- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 16 DILUTION WATER CONTROL: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 18 40 2A 18 40 IB 19 40 2B 19 41 1A 18 41 2A 18 41 IB 18 41 2B 19 42 1A 18 42 2A 18 42 IB 18 42 2B 19 43 1A 18 43 2A 18 43 IB 18 43 2B 19 44 1A 18 44 2A 18 44 IB 18 44 2B 19 45 1A 18 45 2A 18 45 IB 18 45 2B 19 3 1 2 4 5 5 6 7 8 6 8 7 9 8 8 7 9 8 8 7 12 10 11 13 not contain TSCA CB1 e ^ " y s ,, , id D o es -90- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 17 IPA CONTROL: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 18 0 40 2A 18 1 40 IB 17 3 40 2B 16 3 41 1A 18 3 41 2A 18 3 41 IB 17 5 41 2B 16 4 42 1A 18 4 42 2A 18 6 42 IB 17 6 42 2B 16 5 43 1A 18 4 43 2A 18 7 43 IB 17 6 43 2B 16 5 44 1A 18 5 44 2A 18 7 44 IB 17 6 44 2B 16 6 45 1A 18 10 45 2A 18 12 45 IB 17 11 45 2B 16 7 Company Sanitized. Does not contain TSCA CSl -91 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 18 0.63 rng/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 16 5 40 2A 15 3 40 IB 18 2 40 2B 17 4 41 1A 16 8 41 2A 15 7 41 IB 18 5 41 2B 17 8 42 1A 16 6 42 2A 15 7 42 IB 18 6 42 2B 17 8 43 1A 16 7 43 2A 15 7 43 IB 18 6 43 2B 17 8 44 1A 16 7 44 2A 15 7 44 IB 18 7 44 2B 17 8 45 1A 16 10 45 2A 15 13 45 IB 18 8 45 2B 17 11 Company Sanitized. Does not contain TSCA CBl -92- H -24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 19 1.3 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 17 2 40 2A 17 3 40 IB 16 4 40 2B 18 6 41 1A 17 4 41 2A 17 5 41 IB 16 4 41 2B 18 6 42 1A 16 7 42 2A 17 8 42 IB 16 8 42 2B 18 6 43 1A 16 7 43 2A 17 8 43 IB 16 8 43 2B 18 7 44 1A 16 7 44 2A 17 8 44 IB 16 8 44 2B 18 8 45 1A 16 11 45 2A 17 11 45 IB 16 9 45 2B 18 10 CompanySanitized. Dees notcontain 7SCA CBt -93- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 20 2.5 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 17 40 2A 19 40 IB 19 40 2B 17 41 1A 17 41 2A 19 41 IB 19 41 2B 17 42 1A 17 42 2A 19 42 IB 19 42 2B 17 43 1A 17 43 2A 19 43 IB 19 43 2B 17 44 1A 17 44 2A 19 44 IB 19 44 2B 17 45 1A 17 45 2A 19 45 IB 19 45 2B 17 5 3 7 3 6 5 7 4 8 7 9 7 8 8 9 9 9 9 9 9 13 11 12 10 tsc^c 1 n o ic o n ^ 111 $an* -94- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 21 5 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 14 40 2A 12 40 IB 15 40 2B 14 41 1A 14 41 2A 12 41 IB 15 41 2B 14 42 1A 14 42 2A 12 42 IB 15 42 2B 14 43 1A 14 43 2A 12 43 IB 14 43 2B 14 44 1A 14 44 2A 12 44 IB 14 44 2B 14 45 1A 14 45 2A 12 45 IB 14 45 2B 14 4 4 5 4 6 4 6 6 6 4 8 7 7 4 8 7 7 4 8 7 7 7 9 8 Sanff^i -95- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 22 10 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 15 40 2A 15 40 IB 14 40 2B 13 41 1A 15 41 2A 15 41 IB 14 41 2B 13 42 1A 15 42 2A 15 42 IB 14 42 2B 13 43 1A 15 43 2A 15 43 IB 14 43 2B 13 44 1A 15 44 2A 15 44 IB 14 44 2B 13 45 1A 15 45 2A 15 45 IB 14 45 2B 13 1 4 4 2 3 6 5 3 4 5 6 3 5 6 7 4 6 6 7 4 8 7 7 8 Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 23 WATER CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 45 A 15 45 B 15 46 A 15 46 B 15 47 A 15 47 B 15 48 A 15 48 B 15 49 A 15 49 B 15 50 A 15 50 B 15 51 A 15 51 B 15 52 A 15 52 B 15 53 A 15 53 B 15 54 A 15 54 B 15 55 A 15 55 B 15 56 A 15 56 B 15 57 A 15 57 B 15 58 A 15 58 B 15 59 A 15 59 B 15 60 A 15 60 B 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 00 rmes noOcontain t sc a c -97- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 23 (Continued) WATER CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 0 0 15 61 B 15 0 0 15 62 A 15 0 0 15 62 B 15 0 0 15 63 A 15 0 0 15 63 B 15 0 0 15 64 A 15 0 0 15 64 B 15 0 0 15 65 A 15 0 0 15 65 B 15 0 0 15 66 A 15 0 0 15 66 B 15 0 0 15 67 A 15 0 0 15 67 B 15 0 0 15 68 A 15 0 0 15 68 B 15 0 0 15 69 A 15 0 0 15 69 B 15 0 0 15 70 A 15 0 0 15 70 B 15 0 0 15 71 A 15 0 0 15 71 B 15 0 0 15 72 A 15 0 0 15 72 B 15 0 0 15 73 A 15 0 0 15 73 B 15 0 0 15 74 A 15 0 0 15 74 B 15 0 0 15 75 A 15 0 0 15 75 B 15 0 0 15 76 A 15 0 0 15 76 B 15 0 0 15 Company Sanitized. Does not -98- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 23 (Continued) WATER CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 77 A 15 0 0 15 77 B 15 0 0 15 78 A 15 0 0 15 78 B 15 0 0 15 79 A 15 0 0 15 79 B 15 0 0 15 80 A 15 0 0 15 80 B 15 0 0 15 81 A 15 0 0 15 81 " B 15 0 0 15 82 A 15 0 0 15 82 B 15 0 0 15 83 A 15 0 0 15 83 B 15 0 0 15 84 A 15 0 0 15 84 B 15 0 0 15 85 A 15 0 0 15 85 B 15 0 0 15 86 A 15 0 0 15 86 B 15 0 0 15 87 A 15 0 0 15 87 B 15 0 0 15 88 A 15 0 0 15 88 B 15 0 0 15 89 A 15 0 0 15 89 B 15 0 0 15 90 A 15 0 0 15 90 B 15 0 0 15 Company Sanitized. Does not contain TSCA CBi H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 24 IPA CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, ONCORHYNCHUS MYKISS, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 45 A 15 0 0 15 45 B 15 0 0 15 46 A 15 0 0 15 46 B 15 0 0 15 47 A 15 0 0 15 47 B 15 0 0 15 48 A 15 0 0 15 48 B 15 0 0 15 49 A 15 0 0 15 49 B 15 0 0 15 50 A 15 0 0 15 50 B 15 0 0 15 51 A 15 0 0 15 51 B 15 0 0 15 52 A 15 0 0 15 52 B 15 0 0 15 53 A 15 0 0 15 53 B 15 0 0 15 54 A 15 0 0 15 54 B 15 0 0 15 55 A 15 0 0 15 55 B 15 0 0 15 56 A 15 0 0 15 56 B 15 0 0 15 57 A 15 0 0 15 57 B 15 0 0 15 58 A 15 0 0 15 58 B 15 0 0 15 59 A 15 0 0 15 59 B 15 0 0 15 60 A 15 0 0 15 60 B 15 0 0 15 Company Sanitized. Does not contain TSC* C m - 100- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 24 (Continued) IPA CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 61 B 15 62 A 15 62 B 15 63 A 15 63 B 15 64 A 15 64 B 15 65 A 15 65 B 15 66 A 15 66 B 15 67 A 15 67 B 15 68 A 15 68 B 15 69 A 15 69 B 15 70 A 15 70 B 15 71 A 15 71 B 15 72 A 15 72 B 15 73 A 15 73 B 15 74 A 15 74 B 15 75 A 15 75 B 15 76 A 15 76 B 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 Company Sanitized. Does not contain TSCA C B i H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 24 (Continued) IPA CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 77 A 15 0 0 15 77 B 15 0 0 15 78 A 15 0 0 15 78 B 15 0 0 15 79 A 15 0 0 15 79 B 15 0 0 15 80 A 15 0 0 15 80 B 15 0 0 15 81 A 15 0 0 15 81 B 15 0 0 15 82 A 15 0 0 15 82 B 15 0 0 15 83 A 15 0 0 15 83 B 15 0 0 15 84 A 15 0 0 15 84 B 15 0 0 15 85 A 15 0 0 15 85 B 15 0 0 15 86 A 15 0 0 15 86 B 15 0 0 15 87 A 15 0 0 15 87 B 15 0 0 15 88 A 15 0 0 15 88 B 15 0 0 15 89 A 15 0 0 15 89 B 15 0 0 15 90 A 15 0 0 15 90 B 15 0 0 15 Cm pany Sanitized. Does not contain TSCA c m - 102- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 25 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 45 A 15 0 0 15 45 B 15 0 0 15 46 A 15 0 0 15 46 B 15 0 0 15 47 A 15 0 0 15 47 B 15 0 0 15 48 A 15 0 0 15 48 B 15 0 0 15 49 A 15 0 0 15 49 B 15 0 0 15 50 A 15 0 0 15 50 B 15 0 0 15 51 A 15 0 0 15 51 B 15 0 0 15 52 A 15 0 0 15 52 B 15 0 0 15 53 A 15 0 0 15 53 B 15 0 0 15 54 A 15 0 0 15 54 B 15 0 0 15 55 A 15 0 0 15 55 B 15 0 0 15 56 A 15 0 0 15 56 B 15 0 0 15 57 A 15 0 0 15 57 B 15 0 0 15 58 A 15 0 0 15 58 B 15 0 0 15 59 A 15 0 0 15 59 B 15 0 0 15 60 A 15 0 0 15 60 B 15 0 0 15 -103 - Company Sanitized. Does n n t ntaim T S H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 25 (Continued) 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 0 0 15 61 B 15 0 0 15 62 A 15 0 0 15 62 B 15 0 0 15 63 A 15 0 0 15 63 B 15 0 0 15 64 A 15 0 0 15 64 B 15 0 0 15 65 A 15 0 0 15 65 B 15 0 0 15 66 A 15 0 0 15 66 B 15 0 0 15 67 A 15 0 0 15 67 B 15 0 0 15 68 A 15 0 0 15 68 B 15 0 0 15 69 A 15 0 0 15 69 B 15 0 0 15 70 A 15 0 0 15 70 B 15 0 0 15 71 A 15 0 0 15 71 B 15 0 0 15 72 A 15 0 0 15 72 B 15 0 0 15 73 A 15 0 0 15 73 B 15 0 0 15 74 A 15 0 0 15 74 B 15 0 0 15 75 A 15 0 0 15 75 B 15 0 0 15 76 A 15 0 0 15 76 B 15 0 0 15 - 104- Company Sanitized. Does not contain TSOA 6 H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 25 (Continued) 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 77 A 15 0 0 15 77 B 15 0 0 15 78 A 15 0 0 15 78 B 15 0 0 15 79 A 15 0 0 15 79 B 15 0 0 15 80 A 15 0 0 15 80 B 15 0 0 15 81 A 15 0 0 15ld 81 B 15 0 0 15 82 A 15 0 0 15,d 82 B 15 0 0 15 83 A 15 0 0 15ld 83 B 15 0 0 15 84 A 15 0 0 15Id 84 B 15 0 0 15 85 A 15 0 0 15ld 85 B 15 0 0 15 86 A 15 0 0 15ld 86 B 15 0 0 15 87 A 15 0 0 15ld 87 B 15 0 0 15 88 A 15 0 0 15ld 88 B 15 0 0 15 89 A 15 0 0 15ld 89 B 15 0 0 15 90 A 15 0 0 15ld 90 B 15 0 0 15 not contain TSCACB1 Company Sanitized. Does - 105- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 26 1.3 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 45 A 15 0 0 15 45 B 15 0 0 15 46 A 15 0 0 15 46 B 15 0 0 15 47 A 15 0 0 15 47 B 15 0 0 15 48 A 15 0 0 15 48 B 15 0 0 15 49 A 15 0 0 15 49 B 15 0 0 15 50 A 15 0 0 15 50 B 15 0 0 15 51 A 15 0 0 15 51 B 15 0 0 15 52 A 15 0 0 15 52 B 15 0 0 15ld 53 A 15 0 0 15 53 B 15 0 0 15ld 54 A 15 0 0 15 54 B 15 0 0 15ld 55 A 15 0 0 15 55 B 15 0 0 15ld 56 A 15 0 0 15 56 B 15 0 0 15ld 57 A 15 0 0 15 57 B 15 0 0 15ld 58 A 15 0 0 15 58 B 15 0 0 15ld 59 A 15 0 0 15 59 B 15 0 0 15]d 60 A 15 0 0 15 60 B 15 0 0 15ld -106- Company Sanitized. Does not contain TSCA GB1 H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 26 (Continued) 1.3 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 61 B 15 62 A 15 62 B 15 63 A 15 63 B 15 64 A 15 64 B 15 65 A 15 65 B 15 66 A 15 66 B 15 67 A 15 67 B 15 68 A 15 68 B 15 69 A 15 69 B 15 70 A 15 70 B 15 71 A 15 71 B 15 72 A 15 72 B 15 73 A 15 73 B 15 74 A 15 74 B 15 75 A 15 75 B 15 76 A 15 76 B 15 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 0o Co*wpan^ Sanitize- 15 15ld 15 15ld 15 15ld 15 15ld 15 15ld 15 15ld 15 15Id 15 15ld 15 15ld 15 15ld 15 15ld 15 15ld 15 15ld 15 15ld 15 15,d 15 ,,Meod&tfttSCA - 107- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 26 (Continued) 1.3 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 77 A 15 0 0 15 77 B 15 0 0 15ld 78 A 15 0 0 15 78 B 15 0 0 15ld 79 A 15 0 0 15 79 B 15 0 0 15ld 80 A 15 0 0 15 80 B 15 0 0 15ld 81 A 15 0 0 15 81 B 15 0 0 15,d 82 A 15 0 0 15 82 B 15 0 0 15Id 83 A 15 0 0 15 83 B 15 0 0 15ld 84 A 15 0 0 15 84 B 15 0 0 15ld 85 A 15 0 0 15 85 B 15 0 0 15ld 86 A 15 0 0 15 86 B 15 0 0 15ld 87 A 15 0 0 15 87 B 15 0 0 15,d 88 A 15 0 0 15 88 B 15 0 0 15Id 89 A 15 0 0 15 89 B 15 0 0 15ld 90 A 15 0 0 15 90 B 15 0 0 15ld /-"-N Company Sanitized. Does not contain TSCACB1 - 108- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 27 2.5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 45 A 15 45 B 15 46 A 15 46 B 15 47 A 15 47 B 15 48 A 15 48 B 15 49 A 15 49 B 15 50 A 15 50 B 15 51 A 15 51 B 15 52 A 15 52 B 15 53 A 15 53 B 15 54 A 15 54 B 15 55 A 15 55 B 15 56 A 15 56 B 15 57 A 15 57 B 15 58 A 15 58 B 15 59 A 15 59 B 15 60 A 15 60 B 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 Company Sanitized. Does no! contain t s > H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 27 (Continued) 2.5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 0 0 15 61 B 15 0 0 15 62 A 15 0 0 15 62 B 15 0 0 15 63 A 15 0 0 15 63 B 15 0 0 15 64 A 15 0 0 15 64 B 15 0 0 15 65 A 15 0 0 15 65 B 15 0 0 15 66 A 15 0 0 15 66 B 15 0 0 15 67 A 15 0 0 15 67 B 15 0 0 15 68 A 15 0 0 15 68 B 15 0 0 15 69 A 15 0 0 15 69 B 15 0 0 15 70 A 15 0 0 15 70 B 15 0 0 15 71 A 15 0 0 15 71 B 15 0 0 15 72 A 15 0 0 15 72 B 15 0 0 15 73 A 15 0 0 15 73 B 15 0 0 15 74 A 15 0 0 15 74 B 15 0 0 15 75 A 15 0 0 15 75 B 15 0 0 15 76 A 15 0 0 15 76 B 15 0 0 15 -no- Company Sanitized, Poes not contain TSCA raw H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 27 (Continued) 2.5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 77 A 15 0 0 15 77 B 15 0 0 15 78 A 15 0 0 15 78 B 15 0 0 15 79 A 15 0 0 15 79 B 15 0 0 15 80 A 15 0 0 15 80 B 15 0 0 15 81 A 15 0 0 15 81 B 15 0 0 15 82 A 15 0 0 15 82 B 15 0 0 15 83 A 15 0 0 15 83 B 15 0 0 15 84 A 15 0 0 15 84 B 15 0 0 15 85 A 15 0 0 15 85 B 15 0 0 15 86 A 15 0 0 15 86 B 15 0 0 15 87 A 15 0 0 15 87 B 15 0 0 15 88 A 15 0 0 15 88 B 15 0 0 15 89 A 15 0 0 15 89 B 15 0 0 15 90 A 15 0 0 15 90 B 15 0 0 15 Company S anitized Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 28 5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 45 45 46 46 47 47 48 48 49 49 '* 50 50 51 51 52 52 53 53 54 54 55 55 56 56 57 57 58 58 59 59 60 60 A B A B A B A B A B A B A B A B A B A B A B A B A B A B A B A B 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 15 0 0 15 -112- C o ffP ^ y S a n d e d . P o es not cor>tain TSCA CBf H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 28 (Continued) 5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 0 0 15 61 B 15 0 0 15 62 A 15 0 0 15 62 B 15 0 0 15 63 A 15 0 0 15 63 B 15 0 0 15 64 A 15 0 0 15 64 B 15 0 0 15 65 A 15 0 0 15 65 B 15 0 0 15 66 A 15 0 0 15 66 B 15 0 0 15 67 A 15 0 0 15 67 B 15 0 0 15 68 A 15 0 0 15 68 B 15 0 0 15 69 A 15 0 0 15 69 B 15 0 0 15 70 A 15 0 0 15 70 B 15 0 0 15 71 A 15 0 0 15 71 B 15 0 0 15 72 A 15 0 0 15 72 B 15 0 0 15 73 A 15 0 0 15 73 B 15 0 0 15 74 A 15 0 0 15 74 B 15 0 0 15 75 A 15 0 0 15 75 B 15 0 0 15 76 A 15 0 0 15 76 B 15 0 0 15 Company Sanitized. Does not contain TSCA CB -113- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 28 (Continued) 5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 77 A 15 0 0 15 77 B 15 0 0 15 78 A 15 0 0 15 78 B 15 0 0 15 79 A 15 0 0 15 79 B 15 0 0 15 80 A 15 0 0 15 80 B 15 0 0 15 81 A 15 0 0 15 81 B 15 0 0 15ld 82 A 15 0 0 15 82 B 15 0 0 15Id 83 A 15 0 0 15 83 B 15 0 0 15ld 84 A 15 0 0 15 84 B 15 0 0 15Id 85 A 15 0 0 15 85 B 15 0 0 15ld 86 A 15 0 0 15 86 B 15 0 0 15ld 87 A 15 0 0 15 87 B 15 0 0 15ld 88 A 15 0 0 15 88 B 15 0 0 15ld 89 A 15 0 0 15 89 B 15 0 0 15ld 90 A 15 0 0 15 90 B 15 0 0 15ld - 114- Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 29 10 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 45 A 15 45 B 15 46 A 15 46 B 15 47 A 15 47 B 15 48 A 15 48 B 15 49 A 15 49 B 15 50 A 15 50 B 15 51 A 15 51 B 15 52 A 15 52 B 15 53 A 15 53 B 15 54 A 15 54 B 15 55 A 15 55 B 15 56 A 15 56 B 15 57 A 15 57 B 15 58 A 15 58 B 15 59 A 15 59 B 15 60 A 15 60 B 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 152d 0 0 15 0 0 152d 0 0 15 0 0 152d 0 0 15 0 0 152d 0 0 15 0 0 152d 0 0 15 0 0 152d 0 0 15 Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 29 (Continued) 10 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 0 0 152d 61 B 15 0 0 15 62 A 15 0 0 152d 62 B 15 0 0 15 63 A 15 0 0 152d 63 B 15 0 0 15 64 A 15 0 0 152d 64 B 15 0 0 15 65 A 15 0 0 152d 65 B 15 0 0 15 66 A 15 0 0 152d 66 B 15 0 0 15 67 A 15 0 0 152d 67 B 15 0 0 15 68 A 15 0 0 152d 68 B 15 0 0 15 69 A 15 0 0 152d 69 B 15 0 0 15 70 A 15 0 0 152d 70 B 15 0 0 15 71 A 15 0 0 152d 71 B 15 0 0 15 72 A 15 0 0 152d 72 B 15 0 0 15 73 A 15 0 0 153d 73 B 15 0 0 15 74 A 15 0 0 153d 74 B 15 0 0 15 75 A 15 0 0 153d 75 B 15 0 0 15 76 A 15 0 0 153d 76 B 15 0 0 15 Company Sanitized. Does not contain' -116- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 29 (Continued) 10 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 77 A 15 77 B 15 78 A 15 78 B 15 79 A 15 79 B 15 80 A 15 80 B 15 81 A 15 81 B 15 82 A 15 82 B 15 83 A 15 83 B 15 84 A 15 84 B 15 85 A 15 85 B 15 86 A 15 86 B 15 87 A 15 87 B 15 88 A 15 88 B 15 89 A 15 89 B 15 90 A 15 90 B 15 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 0 121" 00 0 12f 00 0 12f 00 0 12f t Non-test substance related deaths caused by clogged delivery tube and low dissolved oxygen (see Results section), d Small in size. 153d 15 153d 15 153d 15 153d 15 153d 15 153d 15 153d 15 153d 15 153d 15 153d 15 153d 3 153d 3 153d 3 153d 3 S 5 e ^ p * f Sraufoed. D oes net egrtfafn TSCA CSf H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 30 DILUTION WATER CONTROL: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Replicate Standard Length (cm) Wet Weight (grams) 1A 2A 3A 4A 5A 6A 7A 8A 9A 10 A 11 A 12 A 13 A 14 A 15 A 1B 2B 3B 4B 5B 6B 7B 8B 9B 10 B 11 B 12 B 13 B 14 B 15 B 4.6 1.4904 4.4 1.1711 4.3 1.2471 4.3 1.0174 4.4 1.1610 4.5 1.3020 4.6 1.2409 4.4 1.2004 4.2 0.9793 5.1 1.9465 4.3 0.9406 4.2 1.1231 4.4 1.2142 4.6 1.3685 5.3 2.2767 4.8 1.3968 3.8 0.6880 4.1 1.0628 4.0 0.9886 4.6 1.2712 4.5 1.2043 5.1 1.6860 4.5 1.2902 4.6 1.5201 4.8 1.4571 4.9 1.5656 4.5 1.2401 5.1 1.8431 4.7 1.3717 4.6 1.3117 Company Sanitized. Doss not eonfte T8CACB1 - 118- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 31 IPA CONTROL: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Replicate Standard Length (cm) Wet Weight (grams) 1A 2A 3A 4A 5A 6A 7A 8A 9A 10 A 11 A 12 A 13 A 14 A 15 A 1B 2B 3B 4B 5B 6B 7B 8B 9B 10 B 11 B 12 B 13 B 14 B 15 B 3.9 4.5 4.6 4.3 4.6 4.7 4.6 4.7 4.6 4.0 5.1 4.9 3.8 4.0 4.8 4.5 4.1 4.7 4.5 5.0 4.2 4.3 3.6 3.9 4.2 4.1 4.6 4.7 5.1 4.1 0.8336 1.4330 1.4479 1.0692 1.3779 1.3918 1.3401 1.4109 1.3379 0.8080 1.9977 1.7902 0.7349 0.9310 1.5750 1.4302 0.9792 1.4042 1.2899 1.8919 1.0669 1.1124 0.6892 0.7861 1.1454 1.0535 1.7020 2.0415 2.2315 1.0868 -119- Company Santttad. Doss not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 32 0.63 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Replicate Standard Length (cm) Wet Weight (grams) 1A 2A 3A 4A 5A 6A 7A 8A 9A 10 A 11 A 12 A 13 A 14 A 15 A 1B 2B 3B 4B 5B 6B 7B 8B 9B 10 B 11 B 12 B 13 B 14 B 15 B 4.4 5.2 4.2 4.6 3.5 4.9 4.1 4.6 3.6 4.4 4.6 4.7 4.1 3.8 4.9 3.7 4.4 5.5 4.6 3.9 4.2 4.9 4.3 3.8 3.8 3.8 4.6 5.2 3.9 4.0 1.2387 2.2000 1.0761 1.2309 0.5266 1.5784 0.9526 1.2650 0.6087 1.2178 1.4816 1.2987 0.9348 0.7409 1.8104 0.7031 1.1193 2.6870 1.4748 0.7402 1.0595 1.8696 1.2407 0.6960 0.8485 0.7256 1.3516 2.0165 0.8067 0.8609 - 120- Company Sanitized, P oes not contain TSC A H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 33 1.3 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Replicate Standard Length (cm) Wet Weight (grams) 1A 2A 3A 4A 5A 6A 7A 8A 9A 10 A 11 A 12 A 13 A 14 A 15 A 1B 2B 3B 4B 5B 6B 7B 8B 9B 10 B 11 B 12 B 13 B 14 B 15 B 4.0 5.3 4.6 3.9 4.1 4.0 3.8 3.9 4.3 4.5 4.7 4.4 5.0 3.4 4.2 4.9 4.9 3.0 3.9 3.7 4.8 3.9 4.3 4.3 4.3 4.6 4.1 4.0 4.5 4.5 0.8851 2.356 1.4227 0.7065 0.8720 0.9329 0.7177 0.7792 1.1371 1.2410 1.5435 1.3841 1.9045 0.5889 1.1222 1.9323 1.6892 0.4331 0.8553 0.8054 1.6577 0.9217 1.3006 1.2228 1.1386 1.2696 1.0364 0.9433 1.3399 1.1592 - 121 - Company Sanitized. Does not contain TSCA CBI H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 34 2.5 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Replicate Standard Length (cm) Wet Weight (grams) 1A 2A 3A 4A 5A 6A 7A 8A 9A 10 A 11 A 12 A 13 A 14 A 15 A 1B 2B 3B 4B 5B 6B 7B 8B 9B 10 B 11 B 12 B 13 B 14 B 15 B 4.4 1.1428 4.5 1.1615 4.6 1.3356 4.2 1.0136 4.5 1.2240 4.6 1.4234 4.6 1.3265 4.1 0.9396 3.8 0.7740 3.5 0.6740 5.2 2.2725 4.4 1.2405 3.7 0.6606 4.6 1.4608 4.5 1.2362 4.8 2.1571 4.2 1.1943 3.7 0.6238 3.6 0.7038 4.3 1.1273 3.8 0.8275 4.7 1.3778 4.1 0.8607 3.6 1.4794 5.5 2.5532 4.1 0.9505 4.0 0.8852 3.6 0.6275 4.2 1.0204 4.5 1.2146 Sanitized. Does no1contain TSC CB1 C om paq H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 35 5 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Replicate Standard Length (cm) Wet Weight (grams) 1A 2A 3A 4A 5A 6A 7A 8A 9A 10 A 11 A 12 A 13 A 14 A 15 A 1B 2B 3B 4B 5B 6B 7B 8B 9B 10 B 11 B 12 B 13 B 14 B 15 B 5.2 2.0702 4.5 1.3127 4.2 1.1007 3.9 0.8384 4.7 1.3550 4.0 1.0300 4.5 1.3834 4.9 1.8640 3.9 0.8269 3.7 0.7091 4.5 1.3704 4.7 1.6631 4.0 0.9759 3.8 0.6888 5.0 1.5518 5.1 1.7325 5.0 1.4996 5.1 2.1433 4.5 1.5103 3.6 0.6885 3.7 0.8663 4.9 1.5771 4.1 1.1297 4.1 1.2107 3.6 0.6339 4.4 1.2599 4.6 1.3984 4.3 0.7385 4.3 1.2696 3.9 0.8574 Company Sanitized. Does not contain TSCA CBI - 123 - H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 TABLE 36 lOmg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Replicate Standard Length (cm) Wet Weight (grams) 1A 5.0 1.7279 2A 4.5 1.3768 3A 5.1 2.3752 4A 4.0 0.9063 5A 3.7 0.6258 6A 3.7 0.7298 7A 3.7 0.6960 8A 3.9 0.8408 9A 3.7 0.6377 10 A 4.0 0.7930 11 A 4.7 1.3288 12 A 4.7 1.4856 13 A 4.5 1.2486 14 A 4.6 1.3468 15 A 4.0 0.8324 1B 4.0 0.9230 2B 4.6 1.5074 3B 4.1 0.9450 4B 3.7a 0.6698a 5B 3.7a 0.8071a 6B 4.1a 1.3133a 7B 4.0a 1.0730a 8B 3.6a 0.7933a 9B 3.5a 0.7510 10 B 4.5a 1.6654 11 B 4.0a 1.0229 12 B 4.6a 1.9418 13 B 4.3a 1.3070 14 B 4.4a 1.3083 15 B 4.1a 1.0943 a These fish died on day 87 due to a clogged delivery tube and low dissolved oxygen concentration. Company Sanitized. Does not contain TSCA C B i - 124- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss D u P on t-11603 TABLE 37 SUMMARY TABLE OF STATISTICAL ENDPOINTS ENDPOINT NOEC MATC (mg/L) (mg/L) Egg hatching Larval survival thinning Larval abnormality thinning Larvae survival test end Larval abnormality test end First day hatching Last day hatching First day swimup Length @ test end Length @ test end Weight test end 2.5 3.5 >10 * > 10 * >10 * 57 >10 * > 10 * > 10 * 0.63 0.90 >10 * 57 * Not calculated/could not be adequately determined LOEC (mg/L) 5 * * * 10 * * * 1.3 * 10 EC50 (mg/L) * * * * * * * * * * * STATISTICAL TEST(S) Cochran-Armitage Cochran-Armitage Cochran-Armitage Cochran-Armitage Cochran-Armitage Jonckheere-Terpstra Mann-Whitney Jonckheere-Terpstra Jonckheere-Terpstra Dunnett's Jonckheere-Terpstra COMMENT Exact analysis Exact analysis Exact analysis Sanitized. Does not contain f SCA G il - 125- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 F IG U R E S Company Sanitized. Does not contain TSCA CSt -126- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 1 PHOTOGRAPH OF AN EMBRYO AT APPROXIMATELY 22-HOURS POST-FERTILIZATION Company Sanltlzodi, Does not eon!afnTsC -127- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynckus mykiss DuPont-11603 FIGURE 2 REPRESENTATIVE ANALYTICAL CALIBRATION STANDARD CURVE FOR H-24678: - 128- Company SanKfeed. Does no! contain TSCA C B H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 2 (Continued) REPRESENTATIVE ANALYTICAL CALIBRATION STANDARD CURVE FOR H-24678: Company Sanitized. Does not contain TSCA CBi -129- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 3 REPRESENTATIVE HPLC CHROMATOGRAM OF A CALIBRATION STANDARD SOLUTION lutes at a retention time of approximately 6.7 minutes. Calibration standard solution contains H-24678 at a concentration of 0.263 mg/L. lutes at a retention time of approximately 5.8 minutes, Calibration standard solution contains H-24678 at a concern ition of 0.263 mg/L. Company Sanitized. Does not contain TSCA CBS - 130- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus myldss DuPont-11603 FIGURE 3 (Continued) REPRESENTATIVE HPLC CHROMATOGRAM OF A CALIBRATION STANDARD SOLUTION blutes at a retention time of approximately 5.3 minutes. Calibration standard solution contains ~H-24678 at a concentration of 0.263 mg/L. Company S m H ta*. D * M ,,,aln TSCA CB( H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 4 REPRESENTATIVE HPLC CHROMATOGRAM OF A DILUTION WATER CONTROL SOLUTION ould elute at a retention time of approximately 6.7 minutes. [I would elute at a retention time of approximately 5.8 minutes. Company Sanitized. Does not c o m * , TSCACBf - 132- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 4 (Continued) REPRESENTATIVE HPLC CHROMATOGRAM OF A DILUTION WATER CONTROL SOLUTION 'ould elute at a retention time of approximately 5.3 minutes. -133- TSCA^ti' H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 5 REPRESENTATIVE HPLC CHROMATOGRAM OF AN IPA WATER CONTROL SOLUTION ould elute at a retention time of approximately 6.7 minutes. would elute at a retention time of approximately 5.8 minutes. Company Satirized. Does not contain TSCACBl - 134- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 5 (Continued) REPRESENTATIVE HPLC CHROMATOGRAM OF A P A WATER CONTROL SOLUTION 6 'ould elute at a retention time of approximately 5.3 minutes. at contain i s c a c m Company Sanffized,DoeSI - 135- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 6 REPRESENTATIVE HPLC CHROMATOGRAM OF A H-24678 TEST SOLUTION 021403b29 Smooth(Mn,2X3) 100-1 15200.4 F1:SiRof1 channel,ESTIC 1.628e+005 7.49 A* _ yw- min 2.00 4.00 6.00 8.00 10.00 elutes at a retention time o f approximately 6.7 minutes. Test solution sample contains H-24678 at a nominal concentration of 0.63 mg/L. _______plutes at a retention time of approximately 5.9 minutes. Test solution sample contains H-24678 at a nominal concentration of 0.63 mg/L. Company Sanitized. Does not contain TSCACBl -136- H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss DuPont-11603 FIGURE 6 (Continued) REPRESENTATIVE HPLC CHROMATOGRAM OF A H-24678 TEST SOLUTION blutes at a retention time of approximately 5.3 minutes. Test solution sample contains H-24678 at a 1nominal concentration of 0.63 mg/L. Company Sanitized. - 137-
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CUNY - The Graduate CenterColumbia University Mailman School of Public Health - Sociomedical Sciences