Document LpamvJOgKQ421nYq0mrKm3Gyd

s5 STTipciAv~s MMporn peu, ssn or sre 894 MEASURING POTENTIAL DERMAL T~,ANSFER OF SURFACE si matin io oo TO PESTICIDE KESIDUE GENERATED F~0M INDOOR FOGGER EE aAn, Torn hon USE. J H Ross, T Thongsin~husak, H Fens, EhdtSe K I Krie~ar. California Department of Food Fem ht IR and Agriculture, Worker Health and Safety Branch, Sacramento, CA. 4pe1r60A 0 sy use pcg ancial deo rmal uns hone Foggor was sonductnd Sch Flos. hunsn A study t0 measure potential 'dermal and veliceety" Filloving aver, Indicated fogging inhalation exposure to a chlorpyrifos/aliethrin home logger was conducted wi~h five hi, man velum=cots. Following label indicated fogging AI ete Yel, led Lot treatment and venting at ide~Cical carpeted STL hotel rooms, human subjects in dosimeter aL rh clothing Were led through a routine of prim Sty be Nod JazzerciseTM exercises. Dosimeter clothing present esses vet consisted of cotton gloves, socks, ~ights and Re Sin dareiss oe on he shirts. Followin~ exercise in each room the acts Senos in cosesated dosiaoer subjects removed the contaminated dosimeter SE a Bt rir eites olo~hin~ and put on fresh clothes. In addition SR to clothing, floor deposition was monitored with~ ~auze and aluminum foil corner pads. SE I Clothing and corner pads were eK~racted with Sh tate eels Sle ethyl acetate and extracts analyzed by electron Cac a Sheena Saleh 55 capture gas chromatography. A majority ~55- Br spr TA 61%) o the fobger insecticides were deposited FX eee on ~he floor. The coefficients for transfer of Shictortiees ma HRT LHI chlorpyrifos and allethrin from floor to Sem itl ie npn a clothing were virtually identical ranging from SI SRN Se Tn 5% on shlr~s to 29% on socks. By susuning the er co WR Sir ee a area under the curve ,~ith respect to time it Tats So edleuiied pee That was possible Co calculaCe upper limit El cumulative dermal .xpcsure as if concacC had reget serge been contnual. CalculaCed absorbed dosages wn Ss ei Ll BLS were 6.8 uE/kg for adults with the assumption that no clothes were worn. ws BACILLUS THORMOmNGS sRAELENs 896 BACILLUS THURINGIENSIS ISRAELENSIS SHER TOXN Arne hE SE CYTOLYTIC TOXIN: MAPPING THE SITE fUFSRIAFNOTR BINDING. Chon SLs amis IMPORTANT FOR BINDING. E Chow, S L!u, and S SSEiIoaDReouter of Bmomoion Unveny of S Gill. Department of Entomology, University of verec.n California, Riverside, CA. Theprspora poc cts prodscedby Bucs The parasporal protein crystals produced by Bacillus Sepderin rec teT k sehnhelmoysh `S Compone ent of "ihhe perotSeinLcrfpalreannd scaan bpe baroknen thuringiensis israelensis have been used effectively against certain insect vectors such as the mosquitos and black flies. The 28kDa protein is an important component of the protein crystal and can be broken S SEh eLIAIn Rms down to an aciive 25kDa pol;,,peptide. Monoclonal a a RE a antibodies had been raised against both the denatured eisai ~.;~tl nat~.ve forms of this 25kDa toxin. Th~ binding of ibe Run oun to col cntdoe ches "~'~I labeled 25kDa toxin to cells could be decreased by cen monoclonal ambos ried gu the by certain monoclonal antibodies raised against the sb ot och ar bors tine native toxih. Twelve different hybridomas producing Sac bods wre sec hd cna Pov such antibodies were selected and cloned, Four of ee Te or ears ren denon these antibodies could not recognize heat denatured fmarpinigetant toxin while the other eight could. The toxins were sree fragmented by treatment with various proteases and gaseous, cyanogen bromide. The smallest fragments fy il te Era detected by the antibodies on a western blot were the 5 aghgns genested oy chymioypin 31d 6kDa fragments generated by chymotD,psin and V.-8 rhnpetaedy protease. Treatment with cyanogen bromide pro- Saeed ore preci bls 50 oe ni" Be duced more precise breaks and fewer bands. The BE TA bn Ver he protons ons 15kDa and 17kDa bands were the predominant ones eas fem from a 16 hr cyanogen bromide reaction and were Eopted by ee ibs Forty oer recognized by these antibodies. Fartiai characterRA ra etn shod te ization of the 15kDa p.optidc has been achieved. The TB Sopa oni one cis moty. Toe 15kDa peptide 5ontmns one cystein moiety. These iea Bmimey Sipno eT foie studies confirm the importance of the sites around the co ning an oc 3s ako cystein moiety in cell bindin~ and toxicity as also Emonsveyceheemid modlicion ss demonstrated by chemical modification studies. ? [ese o[|BBe898 |J PRbeEe fJoB FE fJBiJB J 3 2s ET00g0CeS or osFoo oiu raee, 895 EPIDEMICS OF POISONING CAUSED BY THE CONTAMINATION OF FCODS ~TH PESTICIDES. SSir los TotSaE ini. ars, svi. A Ferrera, J R P CabralD. aservicio de Tox,~_logia, Hospital Clinico, Zaragoza, Spain, and International Agency for Research on Scns Tans er Cancer, Lyon, France. Star wi edsatnwiere sallys coyof Pesticides are used almost universally; many of poyrebiglrey tong fey them have high acute and chronic tcxicities. a ti fas hae 123 0Geos sa of These t%D facts have led to a growing number cf Toni toca Sa dione See poisoning incidents. Such incidents may occur SETA wl tei 2 at the individual or group level and may be Sior SmUa SE TLR CF vier voluntary or accidenta!. From the point of view FEE Esa of public health, accidental epidemics occurring Sefbiicaly ao wot snares periodlcally.are of most interest. Emieitcr St a & piel sce of Contamination of fc~d is a typical source of Choon scenes: a pana the seme chemical accidents. We perused the literature Sor resets of comes doo coeamnaiotn of for reports of epidemics due to contamination of ET han feeds with pesticides. Clinical and obmicioce sepis o thee trims vers epidemiological aspects of these epidemics were Shins, To 62 ne chosen ak etaeh n> studied. The 62 cases chosen are divided into fo Sr ete oF pestis Eo four groups on the basis of implications for ey phos prevention: (a) food contamination during Erooe doce -- 8 cons i transport or storage - 16 cases; (b) a ae rot Bo soit = 10 consumption of seed dressed for sewing - i0 TS ERIE lh, cases; it) accidental addition of pesticides t~"~'~' BL eR ond cemation food - 20 cases; and (d) fo<xl contamination due ou Scientia peace 3 ave to bad agricultural practices - 16 cases. Seti mane Erte ano 1 0 Spezific regulatory measures can be taken to Eon on ee ot tae bie, prevent each type of incident. In addition, EeethdBh soa sha oa structures shoutld be set up in advance so that mrmnmi aly such episodes can be quickly and effici.ently detected and stopped. sor eamuisn or Tomer or a wwe pesto Blois 897 MECHANISM OF TOXICITY OF A UNIQUE PESTICIDE Nfi 3 ETHYLPERFLUOROOCTANE SULFONANIDE (NEPFOS), AND iS Tsar siactucronciave Surowine Re ITS METABOLITE PERFLUOROOCTANE SULFONAMIDE ro 1 sal les Mt? mh ct me. [6 (#FOS) TO ISOLATED RABBIT RENAL CORTICAL MITO- CHONDRIA (RCM). T J Cross and R G Schnell- a tell A HEB mann. Dept. Physiol./Pharmaco]., Coll. Vet. nterase of srg ss, Gh Med., University of Georgia, Athens, GA. HDS is curanly bei>n entatad 33 3 ptr: FBi NEPFOS is currently being evaluated as a pestiSide tor Shr artis re ant ve cide for the red imported fire ant. Previous SR on ee ree doves tht wn studies from this laboratory showed that an Lary eric of ros orks ete rs) early effect of NEPFOS and PFOS on rabbit renal oat he 1 oreomn set [or proximal tubules was a concentration-dependent ist a ore sien fe (5-200 uM) increase in ouabain-insansitive resSiration (ese vo of nis sist wat fs [E piration (BESP). The goal of this study was to Geran soutien She Servet 1s poste fo determine whether the increased RESP resulted Fon taupling oF xfsative pusptornatan (ot G6 from uncoupling of oxidative phosphorylation (OX 5H? E10 TE PHOS). NEPFOS (5-100 uM) and PFOS (0.5-50 uM) ertiad slis Chek of a reiries pr f increased state 4 RESP of RCM respiring on pyruate or skins in he hens Sra vate/malate or succinate in the absence of a Phsphae Seceaar or she orient oii: [E phosphate accepter or in the presence of oligoBE ER Tol re mycin, an inhibitor of FOF1-ATPase. The effect Ph Re Ree of NEPFOS (200]lbl-)~ PFOS (I00 uM), and the known frateropare gL) lm pron sony J protonophore<[CCP~luM), on proton movement by ean a ate on aon est RCM was examine-Q'T, Immediately on addition, PFOS and Firm rr. Osi pois [oh and FCCP, but not NEPFOS, dissipated the proton Flan here rite toe ths prot re 3s gradient. These results show that PFOS acts as rotsionnars na miss OF as 5 ty a protonophore and uncouples OX PHOS by this Pg alarina AA mechanism. The lack of proton movement by CEE ame eu NEPFOS suggests that ~E~FOS may~eed to be al A ai ll metabolized to PFO~to produce cytotcxicity and TT (pron by ne uncoupling o~ oR HHOS. (Supported by VMES, Bey E Univ. Georgia), = E1x4hi0b3it E3 224 Sane| aumnczerooss 3M MN02470063 11440033..00000011