Document LpKeqDQ9jZ2x4kM0k5Q3kok2b

AfL ASL6 - 07A 3 ACUTE TO XIC ITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 519. The test sample is FC203CE (2/21/89). Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:_____________________________ ___________________________ Method: OECD Method 202 and U.S. EPA Method EG-1 Type: Acute static renewal GLP: No Y ear com pleted: 1989 Species: Daphniamagna Supplier: In-house cultures. Initial brood stock from Aquatic Bio Systems, Inc, Fort Collins, CO. A nalytical m onitoring: Temperature, pH, and DO Exposure period: 48-hours Test organism age: <24 hours old S tatistical method: Not given. Test conditions: Dilution w ater: Carbon-filtered well water Dilution w ater chem istry: Tem perature: 20 C D .O .: 9.6 mg/L pH: 8.0 Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentration dosing rate: Daily Exposure vessels: 250 mL Pyrex glass beakers containing 200 mL of test solution and a water depth of 6 cm. Num ber o f replicates: 2 Num ber o f daphnids per replicate: 10 Num ber o f concentrations: one plus a blank control W ater chem istry during the study: pH range (0-48 hours): 001091 8.0 - 8.2 (control exposure) 7.9 - 8.1 (1000 mg/L exposure) Tem perature range (0-48 hours): 21 C Dissolved oxygen range (0-48 hours): 8.5 - 9.6 mg/L (control exposure) 6.8 - 9.6 mg/L (1000 mg/L exposure R E S U L T S ______________________________________________ Nom inal concentrations: Bk control and 1000 mg/L Elem ent values: 48-hour ECso = >1,000 mg/L Element values based on nominal concentrations Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test substance 48-hour EC50 was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. . Paul, Minnesota, 55133 DATA QUALITY_________________________________________:__________ R eliability: Klimisch ranking = 2. Testing meets the criteria for quality testing. However, the study lacks information on purity of the test substance and actual measurements of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G1358-1,1989. OTHER_____________ :_____________________________________________ Last changed: 6/26/00 001092 ENVIRDiWENTAL LABORATORY DAPHNIA TOXICITY DATA SHEET lr a j& s s - i __ TEST TYPF. TESTED (? & -- 2 s /< 2 J /& .. LOT^Jgg_____ TEST ORGANIC g fi& W '& J Z W *61 SOJRCE-^W/K ^ k ^ ^ ^ / ^ W ^ / / / k ^ . DATE REC'EU i/U a fe S - AGE OF TEST ORGANI94S:<24 hrs. old instars (neonates).NO. ORGANISMS PER TREATMEfT-- X--- TEST VESSEL: 250 ml PyrexR glass beaker TEST SOLUTION VOLUME: iOfijnLWATER DEPTH:J u a n . TIME STARTED, 1i DATE STARTED-s S f l l f & j .. DATE COMPLETED ___ DILUTION WATER Carbon-filtered well water (Temp.,,^/?_2C, D.O... _mg/lu pH,,&jQ__ ) ANAT.YSTrT ^ A s / A r ^ T ^ / p / h t e P t i_EC^S* Confidence Limits) ^ jQ Q Q t^ /lj.----------- Test Initial Total 24 Hours -48 Hours Vessel Cone. Amount Total No. pH D.O. Total No PH D.O. No. mg/L Added Dead mg/L Dead rag/L X Control nr. (!o*frol -- 1 looo * ZOO 2 ^ 1000 zoo o o r> .. _ n O flip O n fill % Q o ft.l BEST COPY AVAILABLE Test Temp. C Initials Date JL I ~?)3 s / / x m J L ./ ~7f3 S//3/8J Cannents: UiiuiriuaQ vHferd} I t y . ^ uA^iUrtlP u'+K llVtW . 1 R of. tni^iL^ Test methodology conforms with the following two test protocols: (1) OECD #202 "Daphnia sp., Acute Inmobilization Test and Reproduction Test*'. (2) US-EPA #BG-1 "Daphnid Acute Toxicity Test". 001093