Document Lp6wJ7baYeoRQJb1MxRXRN2p7

AR 226 _ 0922 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twohficFhX-m3a1y06a.ls(o1-be Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 015G. The test sample is FX-3106, PO# $5233-004, EAI 85548, Sumitomo 3M Japan. Current biuntfyolrmeatthieorn, 8in3d.i3ca8t%eswaitteisr,a 1mi0x%tuertehoyfle2n.e3g1l%ycoPlF,O1S.,362%.6S%utdioentehyfloeanmeergl,y0co.l2% xanthan gum, 0.1% 2-biphenylol, sodium salt, tetrahydrate, and 0.05% benzotriazole. cThhaerafcotlelrowiiznegd csounmcmenatrryataipopnlsioefs tiompaurmiitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect the toxicity of the fluorochemical component of the test sample. METHOD: Method: Not given. TGyLpPe:: NAocute static-renewal Year completed: 1985 Species: Pimephales promelas Supplier: Fattg Fish Hatchery, Brady, NE. Analytical monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours Statistical methods: Not applicable. No mortality observed. Test fish age: Juveniles Length and weight: Average length = 33 mm Loading: 0.45 g/L Average weigh=t 0.27 g (wet) Pretreatment: None Test conditions: DDiilluuttiioonn wwaatteerr:chCeamrbiosnt-rfyi:ltered well water AHlakradlinneistsy:: 226200 mmgg//LL aass CCaaCCOOs3 Lighting: NotCognidveunc.tivity: 450 umhosicm tSottohcekdailnudtitoenswtastoelruotinoan wperiegphatr/avtoilounm:eTbhaseist.est material was added directly ~~ Concentrations dosing rate: Solution renewal during the study. `Stability ofthe test chemical solutions: Not noted. NExupmobseurreofvreespsleilcsa:te4s:L 2glass beakers containing 3L test solution. 003015 Numberoffish per replicate: 5 Numberof concentrations: five plus a blank control Water chemistry during the study: pH range (0-96 hours): 77..89--88..22 ((c1o0n0t0romlge/xLpoesxuproes)ure) Temperature range (0-86 hours): 20 C Dissolved oxygen range (0-96 hours): 6.99.6 mg. (control exposure) 6.69.6 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control, 10, 50, 100, 500, and 1000 mg/L Element values: 96-hour LCso=>1000 mg/L ~~ Element values based on nominal concentrations Remarks: Testing was conducted on a mixtureasdescribed in the Test Substance rRmarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS `Thetest sample 96-hour LCs for fatheadminnowwas determined to be >1000 mglL, the highest concentration tested. No mortality or sublethal effects were observed in this concentration. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purityofthe test substance and analytical confirmationofthe amountof fluorochemical proportion in solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C2262, 1985. OTHER meee Last changed: 6/26/00 003016 wero] Static EFNISVHIRUTNOMXEINCTIATLY DLAATBAORSAHTEOERTY 96 Hour Avie DEFINITIVE (FULL-SCALE TEST) x-3106 FOES 5233- coy REAL TYPE TESTRenewa) Stare TESTORGANISMFathead Minnow: sMoAuTrEcReIAELa a(rlEy,STSFEaObuEAAsLh33i75o1n)rSeemipteocn.e0LIm:J2a3san8_=5L"0TB.ATOCIH S1(05./3 AVG.WT. 27 grams AVG. SIZE3.3 FISH DISEASE mama None cm NO. ORGANISMS PER D0SE_S--_1 0ADING. FACTORQ.#5" TEST SOLUTION VO3L_LIU TERM S/ALE L-GL. ASS TANK TINE STARTED 3:45 amy _ DATE STARTED_[0-29- 00 0TT TANK DIMENSIONS: 4 Liven PYREXPGLASS BEAKE DILUTION WATER CARBON-FILTERED WEL 85 WATER (TEMPO DATE COMPLETED // -- 2 --R cC., 0.0.9: 6 ppm, ph_, ) Ran ZR rn [a1r0s|fnCagoi/n1ec"i.afrf|oAatmdoaeutdn|tTa[TGDEaetTadtNoTonppHoD-nT 0-|ToDteaadTo] o ph[0-|0- [Tota p Hap ] Sr as 0| {rome s ] PA[0.0. pon| read pen| "pead pon Tleral BT0 ede| fas]ofaba] 5 alo] lioTselobilal eo Dra]olal] 0 obs val so isol"o lus]obalealo flue] 5 Lolo] 3lieo I a |ePala o leolin]1 rales] pil [seeaToll urf seoliseol os[ry] 0 0 Jbeua]fo0epTroluadl]]|50 Tollud] ffnetetionsv ovie 9loppso095[[Sam 1p0 395 J1 ogZo1 T F8 os= o5 d TCnOMMENTS: Fest Shencll Incl hn cerg hd y 1a5YES!Scams gerd, serie on a SECONDARY EFFECTS = Tempirane -- _ Tank (OTHER THAN MORTALIT EC, Mo Isynptons ofa] or ReactionN s 24T -Hr, R 48To-tHalr, Rumere 7A2f:fHerc.ted e 96:4 al sl T T e Te A Te A T Tee wl sl TN NTN NT NNT TS BEST COPY AVAILABLE 003017 hehewal Static FEINSVHIRTOONXMIECNITTAYL DLAATBAORSATHOEREYT Tree 6 Hour Acute DEFINITIVE (FULL-SCALE TEST) FX-310b POS 5233-004 RC TestRemSe tatu ic ma atel riaTESTEDEAL $554 Sumiore3 Japan AR 33 TEST ORGANISMEasheaddunnewSsaadsngdoBiunSScntee vec'o_{-23-25 07.015 sarcs no AVG. WTQ:27 grams AVG. SIZE3.3 FISH DISEASE meaent_Aone, cm NO. ORGAN0 ISHS P0ER0 DOSE7 S" |OAm DING T FACTORC: $57) TEST TINE SSTOALRUTTEIDON9:V45AOm3L _LDIAU TTEERSN S/ATLE LA-RGL_ TASESDT_AN(K 0-2TA9NK-8DISMaErTMNeSIOcNoSnp:LE4TeLDI,TERAP=YR2EXP--GL8ASSS BEAKER IDINLVUETSITOLNGAWTAOTREGRwro arCgAeRb (B1O1N3-keFlI,WLELTLEWRATEELDRCgo((9T5% ECOMNFI_DERONCcE0.,n0S.)0.9-b pom, pH_2. 2) 20 set tit 7EE EY1 1 PS 2 2]I 7 NE 7 rook fiaitiaif total To0pours | sopours| pos| sap No. | Conc. JAmount [TGTaT No. PH[D.0.J Total No] pH] Total No. D.0-JTotal No] pH[D.0. lio {soloTalo also[iso] Jalon >Plu] 0 bal| 0 0 Val7a]oJoleq] 0 alyd, ilo] 24]seeli0sokoA7l40Pali 0 Biles] 5 Lolo] ffist v>ee |fog3o0rs1oe20er--es fg 2 om e T=e ]ed coments: Individual Weights. Toiial pi of highest cone. ested ios lls =8.5 hem a caw foamy |igod Kble in GRteraifTtBeMmperare SECONDARY EFFECTS (OTHER THANMORTALITY) Tank No. Symptoms or Reactions 24-Hr. Ep Total Number Affected A8-Hr, 72-H) 96-H a2a:2|LT TSe % T TS5 TTz N T To e S ] ] 22-4-- T -- N NST TT eTS . TE 003018 2-10-85 ENVIRONMENTAL LABORATORY FINAL REPORT LAB REQUEST NO. C2466 "QUESTER NAME: RRR PT: 0222 20JECT NO: 06 \TE RECEIVED: 12-02-85 SC: NOVEMBER WELL WATER CHARACTERISTICS CCNTRACT LAB: CONTRACT LAB COST: 0 3% E-L ACURS: 2 EST CMPLT DATE: 12-09-85 DATE COMPLETED: 12-11-85 AMPLE DATE CODE DESCRIPTION TEST RESULT 1 11-25-85 WELL 2 11-28-85 DI WATER cop STANDARD PLATE COUNT TOTAL COLIFORM T0T FLUORIDE (DISTILLED) TOTAL ALKALINITY (PH 4.5) TOTAL HARDNESS vi`TOTAL RESIDUAL CHLORINE AMMONIA NITROGEN = AS N SPECIFIC CONDUCTANCE TOTAL SOLIDS WATER TSOTTAANLDARRDESPILDAUTAEL CCHOLUONRTINE PH SPECIFIC CONDUCTANCE 0.4 MG/L 54 COL/ML NEGATIVE <0.4 MG/L 220 MG/L 260 MG/L 820<0.2 NG/L <0.4 MG/L 450 UMHOS/CM 290 MG/L 1<0c-o2L/MuGLsL 6.7 1.3 UMBOS/CN * = CONTRACT LAB SUBNITTED BYledgganee ater 2nd 003019