Document LgyEnKvv6Rb6KZk1gV49Yd463

ARR26_ 3318 50 AR 226 - 3318 DuPont-11953 ~ TRADE SECRET Study Tide H-25436: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss TEST GUIDELINES: U.S. EPA OPPTS, 850.1400 (1996) U.S. EPA TSCA, EG-11 (1982) OECD TG 210 (1992) AuTHOR: Robert A. Hoke, Ph.D. STUDY COMPLETED ON: July 28, 2004 -- " PERFORMING LABORATORY: ~ HEa.sLkedlulPLoanbtodreatNoermyofuorrsToaxnidcoCloomgypaanndyIndustrial Medicine: NEelkwtaornk,RoDaedl,awP.a0r.e B1o9x71540-0050 i LABORATORY PROJECT ID: DuPant-11953 Sponsor: E.L du Pont deNemoursand Company - Wilmington, Delaware 19898 - USA. --~ . x Vege tof 137 -- Company Sanitized. Does not contain ~~~ cay I SE Te - `GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT `This study was conducted in compliance with U.S. EPA TSCA (40 CFR part 792) Good Laboratory Practice Standards, which are consistent with the OECD Principles of Good Laboratory Practice (as revised in 1997) published in ENV/MC/CHEM(98)17 and MAFF Japan Good Laboratory Practice Standards (59 NohSan Number 3850). Test substance characterization and stability analyses were performed at DuPont Regional Analytical Services (RAS), Deepwater, New Jersey. Noneofthe aforementioned analyses were performed under Good Laboratory Practice Standards; however, the analyses were conducted in pimp compliance with ISO 9002 regulations. Allofthe analyses are considered sufficientforthe Applicant / Sponsor: E.I du Pont de Nemours and Company `Wilmington, Delaware 19898 US.A. - Sty Dini ABGAP 1B Senior ReseRaorbcehrEtcoAt.oxHiockoel,ogPihs.tDa.nd Manager sony deo te Applicant/ Sponsor: `DuPont Representative Date --~ BompanySanitized. Does not contain Tsca cay 205R4a3i6n:bowEaTrrloyuLt,ifOenSctoargheynTcohxuicsimtyys Dupo 11953 -- QUALITY ASSURANCE DOCUMENTATION `The conductofthis stud has been subjected to periodic Quality Assurance inspections. The datesofinspection are indicated below. Phase Audited AuditDates Conduct: July 3,23, 24, 31,2003; August 14, 2003; September 3, 4, 2003; October 3, 2003; December 16, 22, 24, 2003; ~ February 4, 2004 ReportRecords: May 26, 2004; June 1-3, 2004 Dates Reported to Study DiraendMcantageomernt July 23, 2003; October 6, 2003; DFeebcreumabreyr5,162,00242), 24, 2003; June 3, 2004 Reported by: Hobe LC.Meefr TCH. 28-JULY-2004 : Joseph C. Hamill Date : Quality Assurance Auditor ~~ . OT `Company Sanitipzeod.sDnOSoSlcomamTSORTE ~ CERTIFICATION `We, the undersigned, declare that this report provides an accurate evaluation ofdata obtained from this study. Astron: __ (00m h Chaeec-_-- 2J8ULi~2004 setts - OhLlL1 2s 28- Jul ~ 200% isan__lrA pe `RobertA.Hoke,PhD. `SeniorResearchEcotoxicologist and Manager Ry oof ~ verse TA 110.2R5a4i3n6b:oEwaTrrloyuLt,iOenScaorgheynTcoxhiucsitmyykiss Dupont11953 --~ TABLE OF CONTENTS Page GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT ccc? QUALITY ASSURANCE DOCUMENTATION... CERTIFICATION ccssssssssmsssssssssssosssssssssssssssssssodd LIST OF TABLES c.ccuvssmmmssmmmmmsmmmssosmsossssmsssmssmmsmsmnmod La A LIST OF APPENDICES csr STUDY INFORMATION ccs EE INTRODUCTION cssmmmmmmmsmnipsmmppmsssssmssmmmmssssimlty MATERIALS AND METHODS worsensld ~ 5 To SBIR mmm mmm------r3 C.. DIONWAEE...errermsmenemsmsmsesmseneseenemensmemsmsenmennneon 1 F. Sample Preparation and Chemical ADBIYSi ...........ovo.ownrvrmresrenererenrmsmrmrn 16 G. SIBSHOAl ABBIYSIS...rvvrvrsrsrsssnessssnes oneness18 A SINT RS crrmpremmmmmmmenmrgmiimmmm----m----8 Be OL REDOM...rrrsrerrmsmemsmsonesesmnoenenssnonenemsmsmsmsmsennennn 20 . SSH] ATES REO crmsmsmemssmmsssnssmmmsrememsmmemsmsemmmmenern2e] DISCUSSION mmssivsssmimmmsommmmmwmsmsmsissiinispspssspmnmalih CONCLUSION cusimmmmprmmmmmmrsiiomssismmmsimmmimssmmmomnlh RECORDS AND SAMPLE STORAGE ccnald TAUERmmmissspintsmsgmmmnigrnssippsmmmmndth -- -_-- `Company Saniized. Does nol contain TSCA CB! 253 tySue Toyon - LIST OF TABLES orion 2F MEASURp EDCON--C--E--NO--FTHa--RstApINTT--ESIT--SOOLU--NTISO--NS Page 1-28 TES WATERCHEMISTYSETEaDILUmTIONWsATERSCOoNTaRCL FACOVPAMA TFT CYTETSTONERTEE ST i 5. HOFTTEhSTT SOLoUT TIONCTSoFoRrTHa E DAoA YRRAANDTBOOaNW RTROOUTUTr,Orr riT nomliEAFRLYLw . Y 6 DB OOLVER D TYG eCaONTRO SOREESST TORA oy 7. SUMMARY OFHATCHING SURVIVAL ABNORMALITIES,AD SHIPFRO SY o CPaTy OA ATTroTOAY H MANSONTRR OT, OrBTemT,o "HATCHING TOTHINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, 5. BDTON WAITERCOaNyTRO Sr A VTAATT --PA ORT " ~~ 109IB 0P-ADCAOYNRTARIONL.BE OEWMTBRROYUOT,SOUnRcoVArIhVyAnLcIhAusDmySHkiAss,TIECARHLIYLDLNIATFTGEAA-FSOTARGTEHTIREOIEXSIOCDIOIAATYYRTMEASNTOEYY...c..coe. 45. 1.TG3o0mg:EMmBReYOiSURnVIVALAAYNDS HATCHING AS TAFORTHE5TEA%aFAW 1. 0o4T3mg EO SURYAATES TCHICNG DAOTATFOST RITEoIP SANYOY HO A em EAR ICD ATENO RAY. 0 14. 25mg ENBRYO SURAVNIDBVATCAHILNG DAAFORTE AAO 15. 16. "0RTROOUgT,OGnEcroVarBmhRymnYecOrhuSsUmyRkViIssV,AELAARNLDYLHIAFTEC-RHSITNSAGGESATTOXAIACFIOTRYETHE SOAR RAAN O TEST.........ocoee s DSILUTbIONWAiTERCAONTROrL SHRIMAE BPAATTAATFCORRTIHEE3.O0DRYAMAINOBOVTTRORUOT,T wene sres 01nn I3 15. D i pTeYT TAA TROY Raia ----------HT---- 3F 0m: SLEe DDAATTAARFGORRTTHEESoAwYAA BO W TTHROOUT,Grerenin nt ~ 1. 06T3Yml: SIMEDAATTAAFROORRTTUHEESo00wARAaISRONTTROHUTO,T vOver ier -- mp seaDone 7.1 `Ho:2R5a4i3n6b;oEwaTrrloyuL,ifOenSctoargheynTcohxuisctmyykiss Dupont 11953 ~~ 20.E1A.R3 LmgY/LL:IFSE-SWTAIGEDTMAOTXAI-CFIOUTRYTHPTEEST.9.0.-DAYRAINBOW TROUT, Oncorhynchus misms, est] 21. 2E:A5RmLLY:LISFWEI-MST-AUGPEDTAOTXAICFIOTRYTTEHSET.9.0.-DAY RAINBOW TROUT, OncorlynS chis mykiS ss, 22. E5A0RmLglYLL:IFSEW-ISMT-AUGPEDTAOTXAICFIOTRYTTEHSET.0.-DAY RAINBOW TROUTre,vi Onm corhync-- hus mi-- s, --" 23.RDAIILNUBTIOOWN TWRAOTUETR, COnOcNoTrhRyOnLch:usLmAyRkiVsAs,LEMAORRLTYALLIIFET-YSATAFGTEERTOTXHIICNINTIYNTGEFSOTR.T..HE 50-DAY 94 24. TIPRAOUCTO,NOTnRcOoLrh:ynLcAhuRsVmAykLisMsO,RETAARLLIYTLYIAFFET-SETRATGHEITNONXIINCGITFYORTHE 9TES0. -DAY RAINBT OW N 25. O0.n3c0ormhgy/nLc:huLsAmyRkVisAsL, EMAORRLTYALLIIFTEYASFTTAEGRETTHOIXNINCIITNYGFTEOSRT.THE90-DAY RAINBOW TROUT-- , 26. On0.c6o3rhmyLnc:huLsAmyRkVisAsL, MEOARRTLAYLLIIFTEY-ASFTTAEGRETTOHXIINCNIITNYGFTOERSTTHE 9v 0-DAr Y RAIs NBOW TROUT, 103 27. 1O .3 mgN Li LC ARVAO LMMYKR OISSR,H ETARALAY FYTLELIRFN TEI-HSITNTC ANGIEYNTGOH FXIOCRIU TTHYES 90-DAYRTEST...A.......I. NTBROOUA WT, 28. `2O.n5cmogr/hyLn:chLuAsRmyVkAisLsM,OERATRLAYLILTIFYEASFTTAEGRETHTIOXNINCIINTGYFORTHE 9T0E-SDTA.Y.R.AINBOW TROsUT,mn 08 29. `5On0comrhygnchLuAsRmyVkAiLss,MOERARTLAYLILTIYFEASFTTAEGRETTHOIXNINCIINTGYTFOERSTT.H.E 0-DAYRAIwNBOmW TmROUTm, ---- 30. DFIOLRUTTHIEON90W-ADTAEYRRACOINNTBROOWL:TROFIUSTH,LOncEorkyNnchAGuNsDmTyWkEisIHs,GHESTASRLAYTLTIEFSE-TSCTOANGECLTUOSXIICOINTY ~ TER smmmnmmmmmmmmimdineorrrn mma 31. IRPAAICNOBNOTWROTLR:OUFTI,SOHnLcorhEyncNhusAGmNykDiTsWs,EHIEGAHRSTLSY LAITFTEE-SSTTACGOENTCOLXUISCIIOTYNFTEOSRT.T.H..E..9..0-DAY 116 32.R0A3I0NmBg/OLW:TFRISOHUTL,ENOnGcToHrShyAnNchDuWsEmyIkGisHsT, SEAARTLYTLEISFTE.CSOTNACGLEUSTOIXOINCFIOTRYTTHESET.0.-.DAY 117 33.R0A.6I3NmBg/OLW: TFIRSOHULT,ENOGncToHrhSynAcNhuDs WmyEkIiGssH,TESAARLTYTLEISFTE.CSOTNACGLEUSTOIXOINCFIOTRYTTHEEST9.0.-D.AY 118 34.R1A.3ImNLBO:WFTISRHOULTE,NGOnTcHoSrhAynNcDhuWsmEyIkGisHsT,SEAATRLTYELSITFCE-OSNTCALGUESTIOOXNIFCOIRTYTHE 90-DAY TEST... 119 35. R2A.I5 NmgB/LO:WFTIRSOHULT,EOncoNrhAyGNncDhTWusEmIyHGkiHsSTs,SEAATRTLEYLSITFCE-OSNTCALGUESITOOXNIFCOIRTTYTHEES9T0-DAY rrr 120 36. 5R0AImNg/BLO:WFITSRHOULT,EOncNorhAyGNncDhTWisEmIyHkGiHsST,SEAATRLTYELSITFCEO-SNTCALGUESITOOXNIFCOIRTTYTHEEST9.0.-.DAY 121 37.SUMMARYTABLE OFSTATISTICALENDPOINTS... 2 -- Company Sanitized. Doos not contain TSCA CBI TT ---------- t1o.2R5a4i3n6:bowBaTrroyuLt,ieO-nSctoargheynTcohxuicsimtyykiss DuPont-11953 -- LIST OF FIGURES Page 1. PHOTOGRAPH OF AN EMBRYO AT APPROXIMATELY 22-HOURS POST-FERTILIZATION ........ 124 2. A REPRESENTATIVE 5; ICAL CALIBRATcI;ONSTANDARD FOMR--H---25436: 125 3. REPRESENTATIVE HPLC CHROMATOGRAM OF A CALIBRATION STANDARD SOLUTION. ......127 4. REPRESENTATIVE HPLC CHROMATOGRAM OF A DILUTION WATER CONTROL SOLUTION ....129 5. REPRESENTATIVE HPLC CHROMATOGRAM OF A IPA WATER CONTROL SOLUTION. 151 6. REPRESENTATIVE HPLC CHROMATOGRAM OF A H-25436 TEST SOLUTION wc 133 LIST OF APPENDICES A. CERTIFICATE OF ANALYSES. ....... sss-------- Page aml ~~ ~~ Company Sanitized. Does not contain TSCA CBI 5s fHo2R5a4i36n:bowBaTrroyu,LiOecoSralgyenTcohxuicsitkyis Dupont 1953 STUDY INFORMATION 9th Collective Nomenclature: CT] -- [ LH25436 Haskell Number: 25436 CAS Registry Number; (AD "~ f=) Known Impurities: (ND Physical Characteristies: Tan to light brown paste Stability: The test substance appeared to be stable under the conditions ofthe studs; no evidence of instability was observed Study Initiated/Completed; June 3, 2003 / see report cover page) IncLife Intiated/Completed: November 6, 2003 February 4, 2004 : ! Company Sanitized. Does not contain TSCA CB EE -- 25436; EarlyLife Stage Toxicity to Rainbow Trout Oncorlynchus mykiss --~ STUDY PERSONNEL Study Director: RoberAt. Hoke, Ph.D. Primary Technician: Jeffrey T. Turner, Sr. `Animal Culturist: Barbra D. Ferrell B.S. Management: Jeanette M. Erhardt, Ph.D. Analytical Associate: Keith B. Prickett, B.S. Analytical Chemist: Bogdan Szostek, Ph.D. Management: S. Mark Kennedy, Ph.D. Statistical Analysis: John W. Green, Ph.D., Ph.D. Management: A. Mike Kaplan, Ph.D. Ecotoxicology Report Preparation: Wanda F. Dinbokowitz Management: Kim D. Birkmeyer, M.S. --~ DuPont-11953 ~~ Company Sanitized. Does not contain TSCA Bl oe I`o102R5Ra4ai3io6nb:boowwETaTrrrlooyuuLt,iOeOn-nccSoourrighpeynnTccohhxuiuscsitmmyykyisss DuDuoPwonlti. g11s95s3 ~ SUMMARY GUIDELINES: The effect of H-2543 survivalofrainbow troUD OncoryBhuns hmaytkcihsisn,g,emgbrroywotsh, aanldevins, taonxdicfiitnygteersltinignsawcacsoradsasnecsesewditihn athfelaopwp-rtohprroiuagthe,G9o0o-ddayLacbaorrlaytiofrey-stage Practice standards and test guidelines EPA OPPTS 850.1400, OECD 210). (U.S. EPA TSCA EG-11; US. CONCNEONMTIRNAATLIOTNESS:T 0.30, 0.63, 1.3,2.5, and 5.0 mg/L H-25436, dilution water control, isopropyl alcobol (IPA) control MEASURED TEST CONCENTRATIONS; 0.35. 0.71, 1.5,2.8, and 5. oLnmge/LdHe-te2c5t4e3d6in dillon-- water a) nd IPA controls (LOD, 0.0002 mglL) Controls (LOD, 0.00028 mg/Lo)ne detected in diliion water and IPA 0.35,0.71, 1.5,2.8, and 5. oneHd-et2e5ct4e3d6invdiioluJti-- on water-- and IPA| Controls(LOD,0.0004 met) AGEOF TEST ORGANISMS: Approximate2l3y hours post-fertilization attest start SOURCE OF TEST ORGANISMS: bUrnfoeordtifliiszhe)dweegrgesoabntdaisnpeedrfmro(cmoTllreocutteldofdgreomIn3cf.e(mSaulmenaenrd, 1 male Washingion, USA) -TEST DESIGN: pAertoetmalborfyo80cuepm,b2rycouspsweprerereexppliocsaeted,p2errecpolnicceanttesrapteironco(n2c0enetmrbartiyoons) at fceosnttrsotalr,t.theOnfindgaeyrl4i6n,gsafwteerreswtihmin-nuepdhtaodabteaglunofin3t0hefidsihlputeiron water cAnoanlcyetnitcraaltivoenri(f1i5caftiisohnopefrHr-e2pl5i4ca3t6e,co2nrceepnltircaattiesonpserwecsonmceandteratoinont)es.t steosltuteinodn.s Asdadmiptlieodnaolndsaamypl1e,s dwearye0,suobnmciettweedekfloyr,enaanldysoins daasy 90 at -- warranted. . - a Company Saniized. Doesnotcontain 7804 cai `0B2R5a4i3n6b:owEaTrrloyuL,iOnecSotrahgyenTcohxuiscmityys DuPonc11953 `Test solutions were supplied to each replicate test chamber by a ~ continuous flow metering system at a rateofapproximately 7.5L per `hour (approximately 24, 7.5-L test solution volume additions per day). Embryos and alevins were held in relative darkness until day 40 and then held unapd hotoe perr iod of 16 hours light and 8 hours darkness (which included 30 minutesoftransitional light betweenlightand dark intervals) for the remainderofthe study. After thinning, the fingerlings were fed newly-hatched brine shrimp (days 46-54) or newly-hatched brine shrimp and trout chow (days 55-89). Test solutions were maintained between 11.7 and 12.0C (mean 11.8C). Daily observations were made for assessment of numberofdead eggs at hatching, first and last day ofhatching, first day ofswim-up, survival and abnormalities from hatching to thinning, and survival and `abnormalities from thinning to test end. Standard length and blotted wet weightofsurviving fingerlings were determined at test end. `CONCLUSION: The NOEC (No Observable Effect Concentration) for H-25436 ina 90-day early life-stage toxicity study using rainbow trout, Oncorhynchus mykiss, was 1.3 mg/L based on nominal H-25436 concentrations, last day ofhatching and the first-dayofswim-up. The LOEC (lowest observed effect concentration) and MATC (maximum `acceptable toxicant concentration), for the same endpoints were 2.5 ~ and 1.8 mg/L, respectively. The NOEC valueforall other test endpoints was > 5.0 mg/L. STUDY (COMPLETION: (see report cover page) --_ - Lompany Sanitizec. Doss not contain rspcai L`Hoo-2RR5ae4ib3n6bb;oowwETaTrrroloyuu,,LiOOfnenc-coSotrarkgypennTceohxhuiuscsimmtyypkbiissss____ DDwuopmolntis11s953y ~ INTRODUCTION Talheevipnsu,rpaonsdeoffitngheirslisngtsuodyfrwaaisntbooawssterosust,thOenecaorrlyhylnifcehsutsagmeyktiosxisc,itinyoaffHlo-w2-5t4h3ro6ugtoh,em9b0r-ydoasy,est. MATERIALS AND METHODS A. Test Guidelines Except as noted below, the study design complies with the following test guidelines: + US. Environmental Protection Stage Toxicity Test." Agency (EPA) (1982). TSCA EG-11, Fish Early Life + U.S. Environmental Protection Agency (EPA) (1996). OPPTS 850.1400, Fish Early Life Stage Toxicity Test?) + Organisation for Economic Co-Operation and Development (OECD) (1992). TG 210, Fish Early Life Stage Toxicity Test ~ = A1c0o%ns2taCnftotreestmbtrarygoentitcemdpeevrealtouprmeoenft1a0nd212C+w2asC ufsoredafloervtinheanddurfaitnigeornolifntghdeevteeslt,opnmoetnt o(fOthEaCtDus2e1d0)i.n Bmoasstedfaocnilitthieesusfeoorffiesxhisetarilnygitfeem-psetraagteusrteudcioensi,rotlheehqiugihpmpernotb,abwihliitcyhoifs typical ethxecemeiddidnlgeotfhtehgeuidsetuldiyn.e limit for temperature variation obviated temperature adjustment in `This exception did not affect the objectives or the validityofthe study. B. Test Substance `The test conta gubstance, H-25436, was suppliedbythesponsoras a tan to lightbygwnpaste. H-25436 : Te he solubility and stabilityof H-25436 a in Haskell Laboratory well water (LWW) were verified under test conditions based on the analytical recoveriesofthe target analytes. AH-m2a5s4t3e6r isntoack1:4 sosloultuitoino(noStfociksoAp)rowpaylsaplrceophaorle:dHaLt WaWco.nceSnetcroantdiaornoyfstaopcpkrsooxliumtaitoenlsywe5r0emg/mL. --_ prepared by adding pre-determined volumesof Stock A to 500 mL volumetric flasks and | e13e Company Sanitizi ed. Dm osse not ceontam in TSCA CBI {102R5a4i3n6b:owETarloyutL,ifOen-cSotrahgeynTcohxiucsimtyykiss DuPont-11953 ~ brreimnagiinnigngthveofliunamlevooflHumLeWtWo.500AlmlLstwoictkhso1l0u0tmioLnsowfeirseopprroeppyalreadlcoonhoalwaenedklthyebaapsipsrotphrrioautgehout twhiethstnuodyv.isTibhlreopurgehciopuittatteh.e sTtuhdey,1235magn/dm5L0smtgoc/kmsLolsuttoicokn swoalsutciloenasrwaenrdegcolloduidnycaonldorgwoiltdhinnocolor cviosliobrlewiptrehcinpoitvaitse.ibleThpreec3ipmitgat/em.LTaensdt6s.o3lumtgio/nmsLwesrteoccklesaorluatnidoncsolwoerrleescslweairthanndopvailseibgloeld in precipitate throughout the study. approximately 700 grams. The total amountof H-25436 used in the definitive study was C. Dilution Water Dilution water originated from the Haskell Laboratory well which is 378-feetdeep and is cased aasndCaseCaOle;dtboy tbheedrfolcokw.-prTohpeorhtairodnneedssaodfditthieonHoLf CWaWCli.s aTdhjeuHstLteoWdWappisrotxhiemnaatcerlayte1d,00p-a1s4s0edmg/L througah green sand filter to remove iron, and filtered through 50-, 10-, and 0.45-u cartridge tfihlrtoerusgthoargeemdopvoelypvairtniyclulcahtleos.ridTehpeipwiantg.erTishehedaitleudtioorncwhaitlelrediassanaaplpryozperdiattweicaenydedairsltyrfiobrutmeadjor anions and ction, metals, otal organochiorine and organophosphate pesticides, and polychlorinated specifications. biphenyls (Table 1). The dilution water meets OECD" and ASTM criteria and --~ D. Test Organism Culture Rainbow trout, Oncorhynchus mykiss, unfertilized eggs and sperm were purchased from bTrroouotdlofidsghe),wIenrce,rSeucmenievred, iWnasshepianrgattoen.plasEtgiecsbaagnsdwshpiecrhmw(ceorlelekcetpetdofnroimce3(fapepmraolxeiamnatde|lyma5l.6eC) bdautrhi.ngAsphpirpopxinigm.atFegleys20a0ndmsLpoefrtmwheerbeufaflelroswoelduttiooneqcuoinltiabirnatientgoglaypcpirnoe,xiTmRaItSe,lyan1d1.s2odCiiunmawater cohvlaorriiadnefwluaisdatdhdatedcotuoldthiembpaegdecofnetrtaiilniiznatgiotnh.e eTghges.baTghweapsurpploasceeodfbtahcek biunftfoetrhewawsatteorrbeamtohvfeorany approximately 20 minutes. The bagofeggs was taken outofthe water bath and the buffer asdolduetdiotnowtahesbraegmoofveegdg.s.ThTehbeabgaogfwsapsergmenwtalysrroecmkoevdebdacfkroamndthfeorwtahtetor ebavtehnlaynddisittsricbountteenthtes were swpaesramg.aiBnufgfeenrtlsyolruotcikoend(b5a0cmkLa)ndwafsoartlhsoanaddrdeedttoutrthenotbehadge twoahteerlpbaactthivfaotreatbhoeutsp1e5rmm.inuTthees.baTghe eemmbbrryyooss.weTrheereemmborvyeods wfreorme tthheenwartiensrebdatwhitahndcotlhdeHbuLffWerWsotloutrieomnoavnedrsepsiedrumalposupreerdmofanfdofotthheer HdeLbrWisWb,efoinreabweaitnegr btartahns(fe1r2r.e1dCto) tuwndoesrtadianrlkensesssst.eelAptparnkosx,imcaacthelcyon2t3aihnoiunrgs1l0ateLro,feamebrraytoesdwere erxapnodsoumrleywpalsacveedriifniteodebmybrseyloecctuipnsg atorbepergeisnentthaettievset.saTmhpeleeomfbrfeyrotniilcizsetdacggegsat,tphheotboeggrianpnhiinnggo,fand comparing with literature sources. and test chambers. Embryos and larvae were identified by labels on aquaria --- Bompany Sanitzed. Dossnotcontain TSCA CB {1.R2a5i4n36b:owEaTrrlouytL,ifOen-cSotralgeynTcohxuischmyykiss Dupont 11953 --~ E. TestMethods'*" Five test substance concentrations, a dilution water control, and an isopropyl alcohol (IPA) control were used in this study. Nominal test substance concentrations were 0.30, 0.63, 1.3, 2.5, and 5.0 mg/L. The concentrationofIPA in al test solutions, with the exceptionofthe dilution water control which contained no solvent, was < 0.1 mL/L. Test chambers (2 replicate chambers per concentration) were 13-L stainless steel aquaria (30 [length] x 14.5 [width] x 30 [height] cm), each holding approximately 7.5 litersofsolution (16.5-crm liquid depth) and fitted with a screen mesh-covered overflow pipe. A recirculating waterbath was used to maintain temperature in the test chambers at 10 2C. Test chambers were positioned in the waterbath using random numbers. Test solutions were supplied to the test chambers under flow-through conditions by a continuous flow metering system. Metering system surfaces exposed to test solutions consistedofglass, `Tygon, and Teflon. The test substance metering system consisted ofa Rainin Rabbit-PlusTM peristaltic pump that delivered the stock solutions and IPA control continuously to the mixing chambersof thetest system at approximately 1.5 mLper hour. Test solutions in the mixing chamber were stirred by amini mixer. Test soluwetreideolivnersed continuously from the mixing chamber to each replicate test chamber at approximately 7.5 L per hour (approximately 24 test solution volume additions per day). Checksofthe flow rate into each replicate chamber `were madeprior to test startand afte test end. ~ Two 212-mL glass embryo cups (5.5-cm diameter) with screen mesh bottoms attached with silicone adhesive were suspendedineach replicate. Twenty embryos were placed into each embryo cup (totalof40 embryos per replicate and 80 embryos per test concentration). Embryo cups were assigned to each replicate and embryos were assigned to embryo cups using random numbers. Embryo cups oscillated at approximately 4 rpm (2 to 7-cm water depth in embryo cup). Embryo cups were inspecteddailyand unfertilized eggs and damaged or fungus-infected embryos were removed. The criterion for embryo death was opaque appearance. `The surviving alevins and fingerlings were thinned to 15 per replicate using random numbers and released into the appropriate test chamber replicate on day 46 after swim-up had begun in the dilution water control. Fish that swim-up are those that occupy and are able to maintain their position in the tophalf ofthe water column and in which the yolk sac has mostly been resorbed. `Test organisms thinned from the test chambers were humanely sacrificed with MS-222 and stored frozen for potential residue analysis ata later date. Onday46, feeding was initiated. The fingerlings were fed newly-hatched brine shrimp (4rtemia sp., San Francisco Bay Brand, Newark, California) 3 times daily on weekdays and twice daily on weekends and holidays, except on days 46 and 47 when they were fed only once and twice, respectively. On day 55 through dStaayrt8e9r, Foinnegeofrtlihneg 3da0i0lySDfe0e3di(nPgsMIwitNhutbrriitnieosnhIrnitmerpnawtaisonrale,plLaLcCe)d.byTahefefeidnignegrlwiintghs Aqua Max were last fed `on day 89 approximately 24 hours prior to test conclusion on the following day. Brine shrimp athnadt AwqouuladMbaexexpaercetaendatloyzaefdfepcetrtihoedisccailelntyitfoicasinstuergeritthyatocfaontstaumdiyn.anUtnseaarteennoftopordesaenndt/oart levels. debris ~ `were removed as needed. The numberoflive fingerlings was determined daily by actual count. EE Bompanysanitized. Does not contain Tse car 2to5R4a3i6n:bowEaTrrloyutL,ifOenSctoargheynTcohxuisctmyykiss DuPont 11953 "The criteria for alevin and fingerling death were the absenceof opercular movement and lack of ~ raetatcesttioenntdowgeerntelehupmraodndeilnyg.sacOrrigfiacneidsumssinrgemMoSv-e2d22b.y tMheiansniunrgemoendntasyw4e6reanmdasduerovfivliennggotrhgaanndisms blotted wet weightofsurviving organisms at test end. In addition, all test organisms sacrificed at thinningonday 46 and three fish from each test chamber at test end were frozen for potential residue analysis at a later date. Diluter operation was checked twice daily. Test solution concentrations were verified by. chemical analysis prior to beginning the test,a test start, weekly, and at test end. Before thinning, water samples were taken from a point between the 2 embryo cups in each replicate test chamber (2 samples per concentration plus back-up samples). After thinning, water samples were taken at mid-depth from each test chamber replicate (2 samples per concentration plus back-up samples). Test solutions were maintained between 11.7 and 12.0C (mea=n 11.8C). `The dillter was wrapped in black plastic to keep the embryos and alevins in relative darkness until the completionofhatching. The black plastic was removed from day 40 onwards. A photoperiodof 16 hours light (310 - 634 Lux)and 8 hours darkness, which included 30 minutes oftransitional light (approximately 26 -- 198 Lux) between the light and dark intervals, was used throughout the remainderofthe study. Dissolved oxygen, pH, and temperature were measured in both replicatesofthe dilution water control, IPA control, and all test concentrations at the beginningofthe test before embryos were. added, weekly, and at test end. Acontinuously-recording thermometer was used to check temperature variations in the test system waterbath. Total alkalinity, EDTA hardness, and ~ `tchoenhdiugchtievsitttyewsterseubmsetaasncuerecdonicnentthera"tAio"nrweiptlihcsatuerovfivtihneg dtiesltutoirognawnaitsemrscaotnttrhoel,beIgPiAnncionngtoroflt,haentdest, weekly, and at test end. Test solutions werenot aerated during the study. F. Sample Preparation and Chemical Analysis 1. Sample Collection and Treatment Analytical determinationofH-25436 concentrations in test solution samples was performed with swaemepklleysacftoelrletcetstedinfirtioamticoancnhtrleplteisctatteertmeistnacthioanm.beAr pprriiomratroytsesatmipnliteipatliuosn,aabtatcekst-uipnistiaamtipolne, awnads collected from cach replicate test chamber. All samples were diluted 1:1 with acetonitrile (5 mL otfrasnasmfeprlreedatdodaeudttoosa5mmplLeracveatolnsitfroirlea)naaltystihse btiymLeoCf/MsSa.mplAiddnigt.ioAnlailqluyo,ttshoeffaolllltoewsitnsgolsuatmipolnesswewreere diluted with 50/50%acetonitrileHLWW before analysis: 2.5 mg/L (diluted 1:2), 5.0 mg/L (diluted 1:4). Backup samples were stored at room temperature. --_ - Lompany Sanitized.Dossnotcontain TSCA CBI fo2R5a4i3n6b:owEaTrrloyt,LiOfnecSotrahgeynTcohiucstmyis Dupont11953 --~ 2. Instrument and Conditions HPLC Instrument: Model 2795, Waters MS Instrument: 2Q, Micromass CoLlCuPmanra:meters: Mobile Phase: LunAa-32u5C1m8M2)a,m2m0omnmiumx 2acmelmate in water BC-2a5cetmonMitraimlemonium acetate in 20/80% water/acetonitile TGriamdeie(nmti:n) %A %B %C Flow (mLimin) 0 0 0 6 03 565 00 00 11000 0033 86551 00 11000 00 0033 18251 00 00 66 0033 Column Temperature: ~~ 30C _ CIonjleucrtainonSVwiotlcuhm:e: 31.52u5Land 7 min MToSniPzaatriaomnetmeordse:: Electrospray, positive ions CCaopniellvaorlytavgoel:tage: 3350KvkV Source Temperature: 120C Desolvation Temperature: 300C Data Acquisition Function: -- min of 690.5, 734.5, 778.6, 822.6, 866.6 m/z; Tp Pin-- i of 790.5, 834.6, 878.6, 922.7, 966.7 mz; . C10o 66.7 mp /z; 0-p 7 min, : of 890.6, 934.6,978.7, 10227, . 5. Quantitation 25436 "The major compgneiven OF the most inten individ czg ro ionized in positive ion mode selected for analysis g d CleCiToSPIEy Precominaicly _-- Company Sanitized. Doss not contain TSCA CBI 1to.2R5a4i3n6b:owEaTrrloyutL,iOfnec-oSragheynTcohxiucsitmyykiss Dupo 11953 2 the ammonium fon adducts when the mobile phase contains a significant concentration of --~ ammonium acetate. Quaggitation was based on the total signal obtained for the ect Separate calibration curves were made for each gr ("The--total -capoweneirraetiomnonofitortheedte.st substance (F-25436) was assigned (0 each group. A stock solutionofthe reference standard, ast GE vesmade by dissolving approximately 10 mgofthe standard in 10mL of acetonitrile. Appropriate aliquotsofthe stock solution were diluted with 50/50% acetonitrile/HLWW to prepare calibration standard solutions with concentrations ranging from 0.103 to 1.04 mg/L. Duplicate injectionsoftest and calibration standard solutions were made and peak areas were determined electronically. `The calibration standard curves were generatedbyregression analysisofthe peak area data from. chromatographyofthe calibration standard solutions. Datafortest solutions were compared to the calibration standard curve to determine concentrations ofH-25436. The limitofdetection (LOD) and limitof quantitation (LOQ) were determined by calculating the average noise level in chromatogramsofthe water and IPA control solution and comparing them to the signal ofa calibration standardofknown concentration. Two chromatograms were examined for noise related peaks near the retention timeoftheanalyte. The LOD was calculated as 3 times the concentration equivalentofthe mean noise level. The LOQ was calculated as 10 times the concentration equivalentofthe mean noise level. -- G. Statistical Analysis `The NOEC for each response was determined through the useoftrend tests (Cochran-Armitage rend test or the Jonckheere trend test) wherever appropriate. These trend tests assume `monotonicity in the concentration-response. Parametric, orifappropriate, non-parametric tests, were used to determineifthere was significant departure from monotonicity. Other multiple comparison methods were used for non-monotone data. Hatching, mortality and larval abnormality data were evaluated by the Cochran-Ammitage test used in a step-down manner with equally-spaced concentration scores.Ifthere was significant departure from monotonicity, then Fisher's exact test was used with a Bonferonni correction." Continuous or reproduction data (length, weight, first and last dayofhatching, first day of " swimup) were evaluated for normality using the Shapiro-Wilk test while homogeneity of - variance was assessed by Levene's test." Data found to be both normal and homogenous were evaluated in the context of ANOVA." Monotonic concentration-response data were evaluated using the Jonckheere-Terpstra trend test." Non-monotonic concentration-response data were `assessed using the Dunnett method"ifthe data were normally distributed and homogeneous; using the Tamhane-Dunnett (or T3) methodTMifthe data were normally distributed but heterogeneous. Iffactors in addition to concentration were considered, such as the number of specimens in the test chamber at est start, then the Jonckheere test was not appropriate and --_ mult-factor ANOVA was used for analysis. Non-normal data were analyzed by aliemative --_-- CompanySenitized. Dossnotcontain TSA CBi Bto2R5a43i6n:bowEaTrrloyuL,ifOen-cSotrahgeynTcahvuiscimtyykiss Dupont 11553 `methods.") Ifpossible, a transformation was found to obtain normality. Otherwise, a Kruskal- ~~ T`Wearlplsitsratetsestt"wraesplstaicleldaptphleicAaNblOeV, Aex,ceDputnans'snotteeds.t" OruetplliaecresdwDeurnenedtett'esr,mainndedthbeyJtohnecTkuhekeerye-outlier rule and, ifpresent, thir effect on the conclusions was determined. IffomradsastaivaenatliyessiwseArenperxeascetntveinrsaiocnoonfttinhueouKsrurseksaplo-nWsae,lleisxatecsttw)apserumsuetda,tifoonllmoewtehdobdys ewxearcet used vceormspiaornissoofnJsoonfcekahceherter-eTaetrmpesnttragrooruspeptaortahtee dMialuntni-onWwhaittenrecyon"tr(oolr,, uesqiuinvgaaleBnotlnyf,erWoinlncioxcoornr)ection. Wrehseproensveesr, paodsjsuisbtlien,gdfeotrerdmiliuntaitoinownaotfetrheconEtCrsoyl wmoarstadliotnye. byWhperorbeitpraonbailtysaniaslysfiosr aflalilferd,eqaumeoncvying- average used to angle or estimate binary estimation procedure'? was used the LCso for length and other continuous for analysis. responses,if Inverse regression appropriate. was The No Observable Effect Concentration (NOEC) was defined as the highest measured. concentration at or below which no statistically significant effect was observed. The Lowest Observed Effect Concentration (LOEC) was the lowest measured concentration at which a statistically significant effect occurred relative to the dilution water control. The maximum acceptable toxicant concentration (MATC) was defined as the geometric meanofthe NOEC and LOEC. -- RESULTS A. Analytical Report 1. Chromatographic Results [ 1.97 minut luted as a wel-resolved peak with a retention timeofapproximately utead a wellresolved peak witha retention time of `approximately 3.50 Minutes. luted as 2wellresolved peak with a retention timeofapproxi 9 minutes. Fig2ushroew de calibrationcurves obtained Chromatogramsofa typical calibration standard are shown in Figure 3. Figures 4 through 6 show chromatograms ofa water blank control solution, IPA blank control solution, and fest concentration solution, respectively. The LOD and LOQ oA were determined to be 0.0002 0.00067 mg/L of H-25436, respectively. The LOD and LOQ f ere detgrmined to be 0.00028ng/L and 0.00093 mg/Lof H-25436, respectively. TheLOD and LOQ fo ere determinteod be 0.0004 mg/L and 0.0013 mg/L of H-25436, respectively. --_-- BompanySanitized. Does not contain Tsca af 2105R4a3i6o;boEwaTrrloyuLt,ifOenSctoargheynTcohxiucsimtyykiss DuPont11953 2. Test Solution Results ~~ `The measured conggnirations of H=25438 for each sampling day are shown in Table 2. Mean `measured values of ged frog L0dex] 17%ofthe targeted nor ms not &gusted for test subSBince puri Mean measured values -- Ta ed frome] 00 0 115%ofthe target concent not adjusted for test substance puri Mean measured valueso ged fi `1m1e7as%ourfetdhev.alues of H-`n2d5m4i3n6alwceornecewnittrhaitnio1n.s5 nXootfatdhjeuslteodwefsotr vtaesltuesufbosrtaalnlcreeplicategiSlampl} ) within a concentration with the exception ofa single value on day 34. These data indicate that the H-25436 concentrations were maintained at acceptable levels throughout the definitive test. Control solutions showed no detectable concentrationsofH-25436 (Table 2). B. In-Life Report Selectionoftest concentrations for the 90-day trout earlylife stage study were based on a o`fpreevgigosusorstcoundtyro.l"teTstwoorginaintiiaslmsd.efiNnoitmiivneasltuHd-ie2s5w4e3r6ectoenrcmeinntartaetidoenasrlsyeldeucetetdofpoorotrhepefirnfaolr9m0a-ndcaey trout early life-stagestudywere 0.30, 0.63].3, 2.5. and 5.0mgfL. The corresponding mean, `measured concentration of H-25436 fr ses were 0.35, 0.71, 1.5, 2.8, --_ [-- id 5.1 ma- g. The cowrerreesp0.o3n4d,ing m0e70a.n1.,5,2.R7e,asaunrded5c.ot03g/L. TihoenscoofHrr-e2s5po4n3d6ingonoiefd, mi concentrationsofH-25436 Be lyses were 0.35, 0.71, 1.5, 2.8, and 5.4 mg/LA dilution water and anIPA contol inthis study. The control solutions showed no detectable levels of H-25436.. Dilution water quality was acceptable based on OECD and ASTM dilution water criteria. Based on the most recent semi-annual dilution water analysis (Table 1), contaminant concentrations were below concentrations that could be expected to affect the integrity ofa study. All chemical and physical parameters in the early lfe-stage study were within expected ranges (Tables 3 t0 6). Alkalinity, hardness, and conductivity ofthe dilution water control, IPA control, `and nominal 5.0 mg/Ltest concentration ranged from 47-52 mg/Las CaCOs, 118-132 mg/Las CaCOs; and 210-282 umhos/em, respectively. Temperature and pHofthetest substance, dilution water, and IPA control solutions ranged from 11.7 to 12.0C (mean 11.8C) and 7.1 to 7.7,respectively. Dissolved oxygen concentrations ranged between 8.6 and 10.7mg/L (80 and 100%ofai saturation based on anairsaturation value of 10.8 mg/L at 12C) over the duration of ptehsetsitcuiddye.s aAnndalPyCsBesS owfebrreinbeelsohwricmopncaenndtrAaqtiuoansMtahxatwionudlicdabteedetxhpaetcttoetadl tooragfafneoccthtlhoerisdcieentific integrityofthe study. A summaryofhatching, swim-up, survival, and abnormalities from hatching to thinning is presented in Table 7. A summaryofsurvival and abnormalities from thinning to test end, and `mean length and wet weight (blotted dry) values at test end is presented in Table 8. Biological --_ observations are presented in Tables 9 fo 36. A photograph ofa representative fertilized egg - Bompany Sanitized. Does not contain TSCA CBI H025R4a3i6n:bowEaTrrloyutL,iOenSctoargheynTcohxiucsimtyykiss DuPont. 11953 used to start the study is presented in Figure 1. Loadings in the Aand B replicatesof the dilution ~~ `cwhaatmerbecorntirno2l4athoteusrtsebnadsewderoen0a.d1a0i6l6y atnudmo0v.e0r99r9agtefiosfh1p8e0rLliotrerappapsrsoixnigmattherloyug2h4e,a7c.h5-tLesttest solution volumes. C. Statistical Analysis Report `The NOEC for number of eggs hatched, numberofabnormal larvae at timeofthinning, number ofabnormal larvae at test end, survivaloflarvae to thinning, survivaloflarvae to test end, all exceeded 5.0 mg/L by the Cochran-Armitage trend test. No ECs values could be computed since the maximum percent effect for anyofthese measures was < 50%. First and last dayofhatching and first day ofswim-up showed massive tis, So that an exact analysis using StatXact was done. All three measures dispalmaonyoteonde trend,sothe NOEC `was determinedbyan exact Jonckheere-Terpstra test in each case. The NOEC for first day of hatching exceeded 5 mg/L while the NOECs for the last dayofhatching and the first day of swim-up were 1.3 mg/L. `The Jonckheere-Terpstra test determined the NOEC for both length and weight ofsurviving troutattestendweregreaterthan 5 mg/L. ~ DISCUSSION `The mean measured concentrationsofH-25436 were maintained at acceptable concentrations throughout the definitive study. The dilution water control and IPA control contained no detectable concentrations of H-25436. The most sensitive endpoints during the study were the ast dayofhatching and the first dayof swim-up (NOEC = 1.3 mg/L) based on the results ofthe Jonckheere-Terpstra trend test. CONCLUSION `The NOEC (0 observable effect concentration) for H-25436 in a 90-day early life-stage toxicity study using rainbow trout, Oncorhynchus mykiss, was 1.3 mg/L based on nominal H-25436 concentrations and the last dayofhatching and the first day ofswim-up. The LOEC (lowest observed effect concentration) and MATC (maximum acceptable toxicant concentration), for the same endpoint were 2.5 and 1.8 mg/L, respectively. -- -_-- EompanySanitized. Does not contain T5CA cai tHo25R4a3i6n;bowEaTrrlouyLti, fOenSctoargheynTcohxiucsimtyiss DuPont 11953 --~ RECORDSANDSAMPLE STORAGE Laboratory-specific or site-specific raw data, such as be retained by the facility where the work was done. personnel files and equipment records will Specimens(if applicable), raw data, and the final report will be retained at Haskell Laboratory, Newark, Delaware, or at Iron Mountain Records Management, Wilmington, Delaware. --~ -- - Company Sanitized. Doss otcontanscACBl Lompany Sanitized. Does not contain ea mag 205R4a3i6n:bowEaTrrloyuLt,ifOen-cSotragheynTcoxhiucsitmyykiss DuPont11953 -- REFERENCES 1. USS. Environmental Toxicity Test. Protection Agency (EPA) (1982). TSCA EG-11, Fish Early Life-Stage 2. U.S. Environmental Protection Agency (EPA) (1996). OPPTS 850.1400, Fish Barly LifeStage Toxicity Test. 3. Organisation for Economic Co-Operationand Development (OECD) (1992). Guideline for the Testing of Chemicals, Number 210: Early-Life Stage Toxicity Test. 4. American Society for Testing and Materials (ASTM) (1992). Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes. Vol. 11.04. E1241-92. Annual Book ofASTMStandards, 5. LCeailtiriftozm,iEa.DaenpdarLtemweisn,toRf.CF.is(h1a97n6d).GaTmreo,utFiasnhdBsualllemtoinn c1u6l4t.ure19(7haptpc.hery methods). 6. Agrest, A. 1990. Categorical Data Analysis, Wiley, New York. -- 7. HYoorckh.berg, Y. and Tamhane, A.C. (1987). Multiple Comparison Procedures, Wiley, New 8. Shapiro, S.S. and Wilk., MB. (1965). An analysisofvariance test for normality (complete: samples). Biometrika 52, 591-611. 9. Box, GEP. (1953). Non-normlity and tests on variances. Biometrika 40, 318-335. 10. Lehmann, E.L (1975). San Francisco. Nonparametrics: Statistical Methods Based on Ranks. Holden-Day, 11. Mehta, C. and Patel, N. (1995). StatXact 3for Windows. Cytel Software Corporation, Cambridge. 12. Stephan, C. E. (1976). Methods for estimating an LCso, Aquatic Toxicology and Hazard Evaluation, Proceedingsofthe First Annual Symposium on Aquatic Toxicology, ASTM - Special Technical Publication 634, American Society for Testing and Materials, Philadelphia. 13. WDirlaepye,r,NNe.Rw. Yaonrdk.Smith, H. (1981).Applied Regression Analysis, 2* edition, pp 266-273. 14. DuPont Fathead Haskell Laboratory (1991). Stati, Minnow, (Pimephalespromelas). Acute96-HourLC: Unpublished rt 3 -- . . --_-- =e EompanySenitized. Does not ceontnainTTeSeCA CBI i1o2R5a4i3n6b:owEaTrroluyLti,fOenScaorgheynTcohxiucshmtyykiss --~ Dupont 11953 -- TABLES company Sonszed. 0%no tain TSCA CB --_ r-- r --------a-- e ee aise e----r ---- ~f , :f Zc2 eosefsscesi5ussgas'ei-n 2 2 ofEoEt:2 : =ei 5 3 I BEZREIEsesiiieatss 1 2 ifFaH; 2 sa5 2| Else 5.11 i ~ | ig:f iepEhmniii3uh3 md,ati2t2t:i2aln2i25ie95nl7, | ggg |k SEREliiiigiiaist 2 =5F ' a EA iE %gE|Zpe3eesi~es sessel2regzel2sf=esnis2]5 gf s 5 2 E5i5s5 4 8: fpossssztven 2230E3EERLEEteal-- iteneil i3 iZEZ EF IEEE FLT 135 ~3i3t ggorgaiiastg liiila lp e aii tileaieide]as amps Sets bose conan TSA Ct 1io2R5a4i3n6:bowEaTrrloyuL,ifOencSotrahgeynTcohxuicsimtyykiss Dupont 11953 -- TABLE2 MEASURED CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS o1n2i5o4a3l Measured Pcncenation Concentration r Diy Diy0 Daye mDau)is Day30 Du Da WWaatteerr CCoonntrrooll BA | NNDD NN O NND N ND NND W ND WND IIPPAA CCoonnttrrooll AB No NNoW NN W NNNNNNWND 00330084 00%% 0o3n 003 ooxm ooxm oomm oonn 0066338A oons ooss oomm o0m oomm ooml ooae 113384 11s6 1133 114 115 51415 114 1s14 -- 225534 2297 2243 2211 2216 2216 22s5 22ss 550084 5532 aB 4 aas s51t aass aass 4as7 company' Sanilized. Dossnotcontain scace! -- N . enerEneeeeeeeeseeeeeaeeoeeseems F10-2R5a4i3n6b:owEaTrrloyutL,ifOencSotargkeynTcoxhiucsitmyykiss DuPone-11953 --~ `TABLE 2 (Conti. nued) MEASURED CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS HNo2m5i4m3a6l Concemnytration Measured oncentration Deydl Day Dass _ 1D2a2ye) Day9 Day76 Days WaWatteerrCCoonnttrrooll AB ND ND ND ND ND ND ND ND nN ND ND ND ND IIPPAACCoonnttrrooll AB ND ND ND ND ND ND ND ND NN ND ND ND ND 0033008A 003344 0033 03 03 03 03 03% 0m 03 03% 03% 035 063A 0638 on 069 on 0% om 0m 0p om om om 07 om 0% om 113384 114s 11s4 113s 1166 1166 I1g6 1167 ~ 225584 2286 2276 2297 3290 3209 3312 3302 550084 4488 489 s5i6 s5t2 5548 65s1 5597 --~ : - (Company Sanilized. Doss not contain TSA 8 _ Io RainbowTrou,Oneorhynchusmybiss `TABLE 2 (Continued) DuPoneli9s3 MEASURED CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS SE, G[one TN. R] [mg 1 Dayso | gL) Recor poms | - z mew | - - E Er E a Nominal 25436 concentration baseodf | alysis. emann --_~ Company Sanitized. Doesnot contain TSCA CBI ET, to Rainbow Trout, Oncorhynchus mykiss ~: TABLE 2 (Continued) DuPont-11953 MEASURED CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS opem TM re Concentration I Emmomemhis | L lwRwOoBow ooOwRB oB3T w oB3ow 2OoBw Xoo3w (mg/L) Day I g/L) Day0 Day 6 Dayl13 Day20 Day27 Day34 ~ 25B 26 21 24 24 23 22 22 ~` Company Sanitized. Does not containTSCA CBI `H025R4a3i6n:bowEaTrrloyutL,iOfnec-oSrugieynTcohxuicsimtyykiss Dupont 11953 ! TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS NH2o5m0a3l6 Concmeentlr)a"tion 0 Diyil Duy oncentstion Duy$s _DwG Due bwi6 Due WWaateerrCCoomnrrooll BA || NNDD. NNOD N NM NNNND N NN N PIPAA CCoontoroll AB Nwo NX O NNNND NN D NNN 00330084 0033 00xx: ooyd o03w% 0o3x 0033% 00334 o06g3a8 | o06ms 0ogm oomm oomm oomm oomm oomm 113384 1134 " "o 1s a 661sus 77 as ~~ 225584 2265 225% 22s7 2299 2289 3312 o3m1 5500a8 a5s0 4489. 5s12 4592 ssoa 5s5s s5e7 ~~ . Company Sanitized. Do nots conts ain TscAcat Er h Cses ue y st po abeionbDee ewnis ~ TABLE 2 (Continued) MEASURED = CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS cNoe omineal, l [2 J -- Mean, Measured i" [we wven enes |1 p|mamys] --gL)' _=Recovery' mraimsi || 0% -: -- I m nmptmsr cr oRae--mBJ--Efeiercseovssetroi E 0 ipEikmre srn5S as5 ccmoenvie cns veibs infm hn Company Sanftized. Does -------- not contain TSCA CBI Bso2R5a4i3n6b:owBTarroyuL:iOfnScaogrenTocxhicuisty mys Duper 11953 rm TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS N2o5m4iz3a6l Measured S-- Concentration DT D0 Dae mDmoT Du Duwi ww WWeaexrCCoomooliBA || NNDX NNDD NMDD NNDD NNDD W ND wND PTPAACCoonmtiAB | N NN N NM D wWNwm W ww w 00330084 00%3 oomn oosn oomx o0m% oomn ooxx ooaaB 003%s o0s0 oomm oome oonm 0oe 0osm 11338a i1ss E3R 1sFE 1E s E1s ws 254 28 24 2s 28 28 21 a7 ~~ 25B 29 24 27 27 27 26 26 Ssoosa s5e7 aar7 ssaa ssss 5s3a ssz ssos --~ . _-- . CompanySanitized. Does not contain TSCA CBI 1.25436: Early LifeStage Tosicty to Rainbow Trout, Oncorhynchus mykiss DuPont-11953 --_ TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS o12r54a3l6 -- = oncentraion : Concentration Dui Dud Du% (nELe) Du@ Dy' Dam WWaatrerCComooniA || NNDD. >ND NNDD NNDD NN D NND WD IIPPAA CCoonnttrrooll AB x> X MW NNNNWND M ND wND 003300A8 0033% 00%3 oosex o0d4s o0e6 0033% 0033s6 0088334 o07m0 oomm oomm oomm 00% o07s% o0z7 113384 1iss W"oooUos a166 11s4 1178 i1s6 --_ 225584 2288 227 2287 3290 2288 3312 o31m 550084 5534 s522 sss assss s5i2 s61s ssee --_ . - . `Company Sanitized. Doss not contain TSCA Cal t1o2R5a4i3n6b:owBTrroyuLti,OfnScoargpencThiosimtiss --_ TABLE 2 (Continued) DuPont 11953 MEASURED CONCENTRATIONS OF H-25436 IN TEST SOLUTIONS CopnrcNeoinmrisaatlon [43 (} : " nee Pieteet [ety mw me) Recovs WWaaesrCCoonnrroallaB || NNDD z- -z FBAaccwumlsa || No =: =- 00330084 003xs 0033ss wiwm o0e6sa 00760 oorr 1in -- 113384 1i3s 15s 1iss 52584 238 22s 2i S5o0s4 5ss 5sa 110088 + NsMeoeamdsiuntoraelcda2vl5all4ie3es6aprciohonrcoemnetsarastiieodn brsattswecdrE eoRn50co evnegrlyb3ysy5ms4ate3yms6f. cibutwoerenot 0N0D0d4enmotels nootfHde2t5e4ct%e6d., The lt ofdelcion "il 4 tMeaainn,emd feormedoy205t4o G3ayc3o.nemration as clelied a te avragofvalves ~c Based on omit H25436concnraion. . -- ! Company Sanitized. Does not contain TSCA CBI t2o5R4a3i6n:bowEaTrrloyu,LiOfen-cSotrahgeynTcohxiucsimtyykiss DuPont11953 -- TABLE3 | WATER CHEMISTRY OF THE DILUTION WATER CONTROL, IPA CONTROL, AND HIGHEST TEST CONCENTRATION WITH SURVIVING ORGANISMS" Total Alkalinity ~~ EDTA Hardness Conductivity Day Replicate (mg/L as CaCO) (mg/L as CaCOy mhos/cm] Dilution Water Control 0A 50 128 270 6A 50 131 270 13 A 49 130 250 20 A 49 128 260 27 A 47 123 240 34 A 50 124 225 a1 A 49 126 220 43 A 49 126 240 55 A 49 127 235 62 A 47 131 20 ~~ 6A 47 76 A 49 123 220 126 210 83 A 50 126 220 9% A 47 18 220 IPA Control 0A 51 6A 50 13 A 50 20 A 50 27 A 50 34 A 51 4A 50 48 A 50 55 A 49 62 A 48 6A 48 6A 49 83 A 51 %A s1 128 280 132 270 129 250 131 265 124 25 124 225 128 25 126 240 126 25 130 230 124 220 127 210 126 225 121 230 -- Company Sanitized. Doss not contain TSA 8 Te 2to5R4a3i6nb:owEaTrroluyLti,fOenSctoargheynTcohxiucsitmyykiss DuPont11953 --_ TABLE 3 (Continued) WATER CHEMISTRY OF THE DILUTION WATER CONTROL, [PA CONTROL, AND HIGHEST TEST CONCENTRATION WITH SURVIVING ORGANISMS Total Alkalinity ~~ EDTA Hardness Day Replicate (mg/Las CaCO;) (mg/L as CaCO; Conductivity mhos/em) Highest Test Concentration with Surviving Organisms -- 5.0 mg/L 0 A 51 129 282 6 A 47 132 270 3A 50 132 252 20 A 50 130 270 27 A 48 123 250 34 A 50 123 230 a1 A 49 126 25 43 A 51 127 250 55 A 52 130 235 62 A 43 131 20 6A 47 124 220 ~ 76 A 49 127 215 8A 51 127 2 9% A 9 120 230 a Based on nominal F-25436 test concentration. -- `Company Sanitized. Doo not contain TSCA CBI Te Ti FtRo-a2R5ia4ni3nb6b;oowwETaTrrrloouyLtui,OfOenncScotorarlghyeynnTecohhxuiucssimtmyybkeisss DuDubPoorwti-1i1o95s3 --_ TABL4E RAINBOTWETMRPOEURTA,TOUnRcoErh(yCn)chOuFsTmyEkSisTs,SEOLAURTLIYOLNISFFEO-SRTTAHGEE T0O-XDIACYITY TEST Dilution Temperature(C) Water IPA [Day Replicate Control Control Nominal Concentrations (mg/L) Water "030 063 13 25 _ 50 _ Bath 0 0 AIL B19 19 119 19 19 ILS 19 19 119 19 119 19 119 122 6 6 A 17 B17 18 17 19 U8 IT 17 117 117 117 18 19 117 122 3A 3B 18 18 18 118 19 118 18 118 17 117 17 119 19 118 122 20 20 A 117 B17 18 17 19 U8 N8 1L8 117 117 117 120 19 18 123 27 A 119 U8 19 18 18 17 119 122 27 34 B18 A 19 18 119 119 120 18 18 118 119 119 118 118 120 122 --~ 3 a B19 A 19 19 19 19 19 120 119 119 119 19 18 119 18 122 4 48 B18 A 119 118 118 119 119 18 118 118 18 18 118 1g 119 122 48 B18 1&1 19 17 17 119 ss $5 AILS 0B 118 18 18 119 18 118 118 118 118 18 118 118 118 123 6 A 19 B19 119 119 19 119 19 19 18 118 19 117 19 1s 122 60 6 A 120 B19 19 19 19 119 19 119 17 118 119 118 119 118 122 76 76 A 19 B19 18 118 19 19 18 19 117 118 19 118 119 119 121 8 8 A 19 B19 18 119 19 119 U8 118 18 117 19 118 Is 118 122 Sf A 0B 1200 120 19 19 120 120 18 19 19 118 119 18 19 119 122 Meanforall concentrations, includingTceomntpreorlsature11(.8C)| Std. Dev.offorall concentrations, including controls 0.1 --_ MaMxiinimmuummfoforralalll ccooncenntcraetinonst,irinncaclluutddiiinnggoccoonnnttsrroo,llss 11.7 12.0 _--m-m Company Sanitized. Does not contain TSA CBY s1o.2R5a4i3n6b:owEaTrrloyu,LiOfneSctoargyenTcohxuiscimtyiss Dupont-11953 -- TABLES PH OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dilution PH Water IPA Nominal Concentrations (mg Day Replicate Control Control __ 0.30 0.63 13 25 5.0 0A 74 74 74 74 73 73 13 0B 74 74 73 73 73 73 73 6A 75 75 75 75 74 74 74 6B 75 7.5 74 74 74 74 74 13 A 7.7 27 7.7 7.7 7.7 76 76 13 B 7.7 77 76 77 76 7.7 76 20 A 75 74 75 74 75 74 74 20 B 75 74 74 74 74 74 74 27 A 74 74 74 73 73 73 73 27 B 74 73 73 73 73 73 73 34 A 74 74 73 73 73 73 73 ~~ 34 B 74 73 73 74 73 73 73 41 A 74 74 74 74 74 74 13 41 B 74 74 74 74 74 73 73 48 A 74 74 73 73 73 73 73 48 B 74 73 73 73 73 73 73 55 A 73 73 73 73 73 72 72 55 B 73 73 73 73 73 72 72 62 A 73 73 73 62 B 73 73 73 73 73 73 72 73 73 72 72 69 A 73 72 73 72 12 72 72 69 B 73 72 72 72 72 72 72 76 A 73 73 72 72 72 72 71 76 B 73 72 72 72 72 72 71 83 A 73 73 73 72 72 72 72 83 B 73 73 73 12 72 72 72 90 A 73 73 73 73 73 73 72 90 B 73 73 73 73 73 72 72 Minimum for all concentrations, including controls 7.1 Maximum for all concentrations, including controls 7.7 --_---_--`Company Sanitized. Doss not contain TSCA CBI 0H-2R5a4i3n6b:oEwaTrrlayuLti,fOenSctoargheyTnocxhiucsitmyykiss DuPont11953 ~~ TABLE 6 90-DDIASYSORLAVIENDBOOXWYTGREONUTC,OONnCcoErNhTynRcAhTusIOmyNki(sms,g/ELA) ROLFYTELSIFTES-OSLTUAGTEIOTNOSXIFCOIRTYTHTEEST Dilution Water IPA Day Replicate Control Control Dissolved Oxygen Concentration (mg/L)* Nominal Concentrations (mg 030 0.63 1.3 25 50 0A 0B 103 104 103 103 104 101 103 103 101 101 100 102 100 99 6 A 104 106 104 104 101 100 102 6B BA 105 105 102 104 103 103 100 107 106 106 105 104 103 105 3B 106 106 104 106 104 105 103 20 A 20 B 106 105 105 103 102 101 101 105 105 102 104 102 103 100 27 A 103103 102 102 101 99 100 27 B 4A 103103 100 102 101 102 101102 100 99 99 100 98 98 ~~ 4B aA 102102 100 104 102101 100 100 100 dol 100 100 98 99 4B 48 A 104 102 101 102 101 101 104 103 102 101 101 101 99 100 8B 104 102 101 101 101 100 99 55 A 55 B 102 101 100 102101 100 100 100 100 99 100 100 99 98 2A 62 B 01 100 99 01 100 99 100 99 99 99 99 99 9.7 9.7 6A B 01 101 99 102 101 99 100 99 og 100 100 99 96 97 6A 76 B 90 90 88 ol 90 88 89 88 88 89 838 88 86 86 8 A 95 94 91 93 93 90 90 8B 90 A 96 95 93 98 93 88 92 93 94 94 93 88 91 88 90 B 91 96 92 89 94 9.1 9.0 Dissolved Oxygen Concentration (mg/L) Mean Std. Dev. for for all all concentrations, concentrations, including including controls controls 9.9 0.5 Minimurn for all concentrations, including controls 8.6 --_ The theoretical disolved oxygeMnacxonicemnutrmatifoonr all concentrations, a saturation at 12C = including 10.8 mplL. controls _10.7 -- es Company Sanitized. Does not contain TSCA GBI .z 2 ~ i5 472458 33253 ~23 3 9 5337 cseslel HE] evel seed ce L. E SdE | 2H1E4 $S2 eF2E05A18T1] free] sosele ssugy cecofel enol] sed ssa Nl nee ommsln 534 | 21 I 3- 223 | FEE 533 zd Bg 5 EH 9 sugy ssiys ssi ceeul| snes EEE TEE nuonwnlel meno nes 22 305]% 1EI ener see anny ': f 2s yF-2 38 | EsNg i3 23 RE. .Z x $3 | & 3 aasspls| s eme ==Ra =ssss ass ssoolsl 2a2ads sees TT ssa 55 | 2 FE sass ssssah5 ssassass g=oeF z g2J sssg2ag3 ssesd sseog 2 = | LHeieg - || f $888 8 g 2 S| a z Company Sanitized. Doss not contain Tara may : : | 3 I| i5 ht :!' 2 89% : 55] weeeeet ood | =e + : = ;i iBgeeE| 3385 S88558 :4 1HE:1ween - Z3: 1%i1 g215 2% Bl amon] os28ss iZ| [I bELsLEEEsy a o xes ~ EL B2E8BE coEeEE so:le po ss " Ei55L53 ; i H4E2 eeeeexiel eenxneln) ooen | 2 p 2 veeconlo wssceele mong ; : | gt gB : i i 5 2Bosses E388 $8854I zi;2 =s1ss%lsl saEEE rySEEYEEI 2+ cH |F" s 4| EEEe| esol oEEE i:: ZF E3 =- ==a samsaa Bawsof 2 :51 1i E 2 = :2 --3 srsss5| ss28d;8 S8 SE]&8 <=sxeqff 5ERER(R] <s35=28/ i : 731 8 z 33 3 : -: a 3 | Company Sanitized. Doss n`ot contain TE" ~ g ~3 2 ww 2 3.g 3 5 ~ig2E:E% 32 g J 373 | J-- : 5 sig 8 SEs ceuge E29X | [22 ,84] one 558 | 55 22 35 | ff E3EEE |: EgEad 5 4 I3 EE =3 FED 225 coud| f : fail BH ! ; IH ig cenel| B 5S 4BFs aEggs | ? 22 33gZI g2z: SESYE]E I 7 emma | fd Fat Bo | 4<85 fy 233 cae| 22]3 15: SE 2225| 22 = u3l semameses | 3&3 3Ii21 az | 2 2:2 Egxa | iI 4 8gneass I$=i: g3 8 7 sazas | giiigs 3:z || E8312s =33J:32 Eompany Saniized. Does notcontainTSCA Col "3| 2 ~ 3 LH22}% EH E$l 3 4 --~Z3 EX @ sBaiEel sEe3l5s s5ols 2B2gg sEe3s3 8 8 Sols SS] S23 29 333 aT5A|RBA2S3 rz 25x | :5 gg E2H5| SBaEsEs als sgl sEgElsE| 883 233 a285l83 323333 I23Ys s3sf3z 3a3y wef wold wold ef wd :f a LaLd eed| wIaolse w93e9 I=IuS weov oS3 I sel sole soy soy wae E Z| 20%, =aERg2E4 LSF2iSEs mofo sale wesaodl ncoells osaolne n}: ET | 82 = Z ofs F3 REE o| of of ol ---o|= 2ge| E814 smd srg ng sg 5 ZI S |ETgEnoe mols noe nee es 2EHB] 12 cole noel malo) cols veee &2S iz 2 - <offl <ofl] offL off <oL zs : 3g3 sied] H33.EIR-z IERIE 283 8 8 1] Company Sanitized. Does not contain TSCA CBI ~ &3 ~~ 2533 3 54 3 EziSgi ~ 2 28 | 2 7) 2EY AEE g 59 2233l7E 2=233888 =g BE?E wala] wos & of | 2 23%3% ggg s38 a2 oFx| gg 22589 283585 2a8 | iEElRE oo ov tg au 3ee 29 5TEE)5a2 1 4 IEE CIEE 925 | %ig2 nol] =v g e% i= 229 =H 3. Ege) fae ; 5S|87 3ff BE sEE| | & =A B= i 8 ig3 23 nolo noe : FFU | 6 4282 B2s) =cul=e] TzEoE || o2FgZ 2agxeS Z FINE4 =i:3 8s 3 <= iF z & ge 2s _of 22Eq :s3z S 3 Company Sanitized. Does not contain TCA ~81 1H0-2R5a4i3n6b:oEwaTrrloyuLti,fOenScoargkeyTnocrhiucsitmyykiss DuPont-11953 --~ TABLES , `TDHIEL9U0T-IDOANYWRAATIENRBOCOWNTTRROOULT:, OEnMcoBrRhyYnOchSuUsRmyVkIisVsA,LEAARNLDYHLAITFCEH-ISTNAGGDEATTOXAIFCOIRTY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 0 1A 0 - 0 20 - - 0 24 0 - 0 20 - - 0 1B 0 - 0 2 - - 0 28 0 - 0 20 - - 1 1A 0 - 0 20 - - 1 24 0 - 0 2 - - 1 1B 0 - 0 20 - - 1 28 0 - 2 1A 0 - 0 20 - - 0 20 - - 2 24 0 - 0 20 - - m 22 218B 0 0 - - 0 20 - - 0 20 . - 3 1A 0 - 0 20 - - 3 24 0 - 0 20 - - 3 1B 0 - 0 20 . - 3 28 0 - 0 2 - - 4 1A 0 - 0 20 - - 4 24 0 - 0 20 - - 4 1B 0 - 0 20 - - 4 28 0 - 0 20 - - 5 iA 0 - 0 20 . - 5 24 0 - 0 20 - - 5 1B 0 - 0 20 - - 5 28 0 - 0 20 - - 6 1A 0 - 6 24 0 - 00 2200 -- .- 6 1B 0 - 0 20 - - 6 28 0 - 7 1A 0 - 0 20 - - 0 20 - - 7 24 0 . 7 iB 0 - 0 20 - - 0 20 - - Pa 7 28 0 - 0 2 - - = --- `Company Sanitized. Does not contin TSCA GF H2R5a4i3n6b:oEwaTrrloyuL,ifOen-cSotragheyTnocxhiucsitmyykiss Dupont 11953 --~ TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Egg Left Larvae Larvae 8 1A 0 - 8 24 0 - 8 1B 0 - 8 28 0 - 9 1A 0 - 9 24 0 - 9 1B 0 - 9 28 0 - 10 1A 0 - 10 2A 0 - ~ 10 1B 0 - 10 28 0 - 11 1A 0 - 1 24 0 - 1 1B 0 - 11 28 0 - 12 1A 1 . 12 2A 0 - 12 1B 0 - 12 28 0 - 13 1A 0 - 13 24 0 - 13 1B 0 - 13 28 0 - 14 1A 0 - 14 24 0 - 14 1B 0 - 14 28 0 - 15 1A 0 - 15 2a 0 - 1s 1B 0 - --_ 15 2B 0 - 0 2 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 1 19 - - 0 20 - - 0 20 - - 0 2 - - 1 19 - - 0 20 - - 0 20 - - 0 20 - - 1 19 - - 0 20 - - 0 20 - - 0 20 - - 1 19 - - 0 20 - - 0 20 - 0 20 z -- -_-- KCompany Sanitized. Doss not contain Ts CE, {H-R2a5i43n6b:owEaTrroluytL,iOfen-cSotrahgeynTcohriucsimtyykiss DuPont11953 -- TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR `THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative 1] Bags Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 16 1A 0 - 16 24 0 - 16 1B 0 - 16 28 0 - 17 1A 0 - 17 24 0 - 17 1B 0 - 17 28 0 - 18 1a 2 - 18 24 4 - ~ 18 1B 2 - 18 2B 4 - 19 1A 0 - 19 24 0 - 19 1B 0 - 19 2B 0 - 20 1A 0 - 20 2a 0 - 20 1B 0 - 20 2B 0 - 21 14 0 - 21 24 0 - 21 1B 0 - 21 2B 0 - 2 1A 0 - | 2 24 0 - 2 1B 0 - 2 28 0 - 2 1A 0 - 2 24 0 - 2 1B 0 - --~ 2 28 0 : 1 19 - - 0 2 - - 0 20 - - 0 20 - - 1 19 - - 0 20 - - 0 20 - - 0 20 - - 3 17 - - 4 16 - - 2 18 - - 4 16 - - 3 17 - - 4 16 - - 2 18 - - 4 16 - 3 17 - -- 4 16 - - 2 18 - - 4 16 - - 3 17 - - 4 16 - - 2 18 - - 4 16 - - 43 1167 -- - 2 18 - B 4 16 - - 3 17 - 4 16 - -- 2 18 - - 4 16 - - -_-- Pompany Sanitized. Does not contain Tscar 25436: Early Life-Stage Toxicity So RaiubowTrout, Oucorapneusmpbiss ooo --~ `TABLE 9 (Continued) DubeliSsy DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Day Replicate' Removed Removed Eggs Leff Larvae Live Larvae 24 1A 0 0 24 2A [J [J 24 1B 0 0 24 2B 0 0 25 1A 0 0 25 2A 0 0 25 1B 0 0 25 2B 0 0 26 1A 0 0 26 2A 0 0 ~ 26 1B 0 0 26 2B 0 0 27 1A 0 0 27 2A 0 [J 27 1B 0 0 27 2B 0 0 28 1A 0 0 28 2A 0 0 28 1B 0 0 28 2B 0 0 29 1A 0 0 29 2A 0 0 29 1B 0 0 29 2B 0 0 30 1A 0 0 .| 30 2A 0 0 30 1B 0 0 30 2B 1 0 31 1A 0 0 31 2A 0 0 31 1B 0 0 ~~ 31 2B 0 0 3 17 - - 4 16 - - 2 18 - - 4 16 - - 3 17 J 0 4 16 0 0 2 18 0 0 4 16 0 0 3 17 0 0 4 16 0 0 2 16 0 2 4 16 0 0 3 16 0 1 4 15 0 1 2 15 0 3 4 16 0 0 3 12 0 5 4 12 0 4 2 7 0 11 4 1 0 5 3 3 0 14 4 6 [J 10 2 2 0 16 4 4 0 12 3 1 0 16 4 5 [1 11 2 1 0 17 5 0 0 15 3 1 0 16 4 0 0 16 2 1 0 17 5 0 0 15 EE Company Sanitized. Doss not contain TSCA 681 125436: EarlyLife Stage Toxicity i BulowTrou,QECOTMIe HIEIIE ne AL25 PN TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR `THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 32 1A 0 1 32 24 0 1 32 1B 0 1 32 2B 0 0 33 1A 0 0 33 2A 0 0 33 1B 0 0 33 2B 0 0 34 1A 0 0 34 24 0 0 ~~ 34 1B 0 0 34 2B 0 0 35 14 0 0 35 2A 0 0 35 1B 0 0 35 2B 0 0 36 1A 0 0 36 24 0 0 36 1B 0 0 36 2B 0 0 37 1A 0 0 37 2A 0 0 37 1B 0 0 37 2B 0 0 38 1A 0 0 38 24 0 0 38 1B 0 0 38 28 0 0 39 1A 0 0 39 24 0 0 39 1B 0 0 "~ 39 2B 0 0 3 0 1 16" 4 0 1 15! 2 0 1 1 5 0 0 15 3 0 1 16 4 0 1 1st 2 0 1 7 s 0 0 15" 3 0 1 16" 4 0 1 15" 2 0 1 7" 5 0 0 15" 3 0 1 16 4 0 1 15M 2 0 1 7 s 0 0 1s! 3 0 1 16" 4 0 1 1st 2 0 1 i" 5 0 0 15" 3 0 1 16" 4 0 1 15! 2 0 1 7 5 0 0 15" 3 0 1 16" 4 0 1 15" 2 0 1 7 5 0 0 15" 3 0 1 16% 4 0 1 15M 2 0 1 im 5 0 0 15 --- Company Sanitized. Doss not contain Sa Caf 1H02R5a4i3n6b:owEaTrrloyuL,ifOencSaargkeynTeohruiscimyykiss Dupont-11953 -- TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Bggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 40 1A 0 1 40 2A 0 0 40 1B 0 0 40 2B 0 0 41 1A 0 0 41 24 0 0 41 1B 0 0 4 28 0 0 a 1a 0 0 42 2A 0 0 ~ a 1B 0 0 42 28 0 0 43 1A 0 0 43 2A 0 0 43 1B 0 0 43 2B 0 0 44 1A 0 0 44 2A 0 0 44 1B 0 0 4" 28 0 0 4s 1A 0 0 as 24 0 0 45 1B 0 0 45 28 0 0 46 1A 0 0 .| 46 24 0 0 46 1B 0 0 46 28 0 0 3 0 2 15 4 0 1 15" 2 0 1 i 5 0 0 15 3 0 2 15 4 0 1 15 2 0 1 7 5 0 0 15" 3 0 2 15% 4 0 1 1s! 2 0 1 17 5 0 0 15! 3 0 2 15* 4 0 1 15! 2 0 1 17 5 0 0 15" 3 0 2 15 4 0 1 1s 2 0 1 17 5 0 0 15 3 0 2 15% 4 0 1 15" 2 0 1 17" 5 0 0 15 3 0 2 15% 4 0 1 1st 2 0 1 hu 5 0 0 15 + SFoourlrosecpultpeisrccocnuaceentdtreastiiso,n (ic, 1A - 28). corretai,and best spina) -- `Company Sanitized. Does not contain TSCA CBI 50- 2105R4ai3n:bowEaTrrloyutL,ifOen-cSotrahgeynTcohruicsimtyykiss DuPont 11953 --~ TABLE 10 IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 0 1A 0 . 0 20 - - 0 24 0 - 0 20 - - 0 1B 0 - 0 20 - - 0 28 0 - 0 2 - - 1 1A 0 - 0 20 - - 1 24 0 - 0 20 - - 1 1B 0 - 0 20 - - 1 28 0 - 0 20 - - 2 1A 0 - 0 20 - - 2 24 0 - 0 20 - - 2 1B 0 - 0 20 - - ~~ 2 28 0 - 0 20 - - 3 1A 0 - 3 24 0 - 0 20 - 0 20 - .- 3 1B 0 - 0 20 - - 3 28 0 . 0 20 - - 4 1A 0 - 0 20 - - 4 24 0 - 0 20 - - 4 1B 0 - 0 2 - - 4 2B 0 - 0 2 - - 5 1A 0 - 0 2 - - 5 24 0 - 0 20 - - 5 1B 0 - 0 20 - - 5 28 0 - 0 20 - - 6 1A 0 - 0 20 - - 6 24 0 - 0 20 - - 6 1B 0 - 0 20 - - 6 28 0 . 0 20 - - 7 1a 0 - 0 20 - - 7 24 0 - 0 20 - - 7 1B 0 - 0 20 - - 7 28 1 - 1 19 - - -- TS-------------- Eompany Sanitized. Doss not contin 72 CBF Hto2R5a43i6n:boEwarTrloyuLt,ifOen-cSoargheynTcoxhiucsimtyykiss DuPont-11953 --~ `TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Egg: LeR Larvae Larvae 3 1A 0 - 8 24 0 - 8 1B 0 - 3 2B 0 - 9 1A 0 - 9 24 0 - 9 1B 0 - 9 28 0 - 10 1A 0 - 10 24 0 - 10 1B 0 - ~ 10 2B 0 - il 1A 0 - 1 24 0 - il 1B 0 - i 28 0 - 12 1A 0 - 12 24 0 - 12 1B 0 - 12 28 0 - 13 1A 0 - 13 24 0 - 13 1B 0 - 13 28 0 - 14 1A 0 - 14 24 0 - 14 1B 1 - 14 28 0 - 1s 14 0 - 15 24 0 - is 1B 0 - is 28 1 - 0 20 - - 0 20 - - 0 20 - - 1 19 - - 0 20 - . 0 20 - - 0 20 - - 1 19 - - 0 20 - - 0 20 - - 0 20 - - 1 19 - - 0 20 - - 0 20 - - 0 20 - - 1 19 - - 0 20 - . 0 20 - - 0 20 - - 1 19 - . 0 20 - - 0 20 - - 0 20 - - 1 19 - - 0 20 - - 0 20 - . 1 19 - - 1 19 - - 0 20 - - 0 20 - - 1 19 - - 2 is - - --_-- = BompanySanitized. Does not contain vr amt H1025R4a3i6n:bowEaTrrlouyLti, fOenSctoargheynTcohruicsitmyykiss DuPont11953 -- `TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eges Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 16 1A 0 - 16 24 0 - 16 1B 0 - 16 28 0 - 17 1A 0 - 17 24 0 - 17 1B 0 - 17 2B 0 - 18 1A 2 - 18 24 2 - 18 1B 2 - 18 28 1 - ~ 19 1A 0 - 19 24 0 - 19 1B 0 - 19 28 0 - 20 1a 0 - 20 24 0 - 2 1B 0 - 20 28 0 - 21 1A 0 - 21 24 0 - 21 1B 0 - 21 28 0 - 2 1A 0 - 2 24 0 - 2 1B 0 - | 2 28 0 - 2 1A 0 - 23 24 0 - 23 1B 0 - 2 28 0 - 0 20 - - 0 20 - - 1 19 - - 2 18 - - 0 20 - - 0 20 - - 1 19 - - 2 18 - - 2 13 - - 2 18 - - 3 17 - - 3 17 - - 2 18 - - 2 18 - - 3 17 - - 3 17 - - 2 18 - - 2 18 - . 3 17 - - 3 17 - - 2 13 - - 2 18 - - 3 17 - - 3 17 - - 2 18 - - 2 18 - . 3 17 - - 3 17 - - 2 18 - - 2 18 - - 3 17 - - 3 17 - : CompanySanitized. Doss not contain TSCA C81 _-------- | || 215R4a3i6n:bowEaTrrloyuL,ifOencSoangfeynTcohsiucsitmyykiss DuPont-11953 PN TABLE 10 (Continued) IRPAACIONNBTORWOTLR:OUETM,BORnYcoOrhSyUnRchVuIsVmAyLkisAsN, DEAHRALTYCHLIIFNEG-SDTAATGAE FTOOXRITCHIETY9T0E-SDATY Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 24 1A 0 - 24 2a 0 - 2 1B 0 - 2 28 0 - 25 1A 0 0 25 24 0 0 25 1B 0 0 25 28 0 0 26 1A 0 0 26 24 0 0 2 1B 0 0 26 28 0 0 ~ 27 1A 0 0 27 24 0 0 27 1B 0 0 27 2B 0 1 28 1A 0 0 28 24 0 0 28 1B 0 0 2 28 0 1 29 1A 0 0 2 24 0 0 29 1B 0 0 29 28 0 0 30 1A 0 0 300 2A 0 0 30 1B 0 0 30 28 0 0 31 1A 0 0 31 24 1 0 31 1B 0 0 31 28 0 0 2 18 - - 2 18 - - 3 17 - - 3 17 - - 2 18 0 0 2 18 0 0 3 17 0 0 3 17 0 0 2 18 0 0 2 18 0 0 3 17 0 0 3 16 0 1 2 16 0 2 2 1 0 7 3 17 0 0 3 12 1 4 2 10 0 3 2 6 0 2 3 9 0 8 3 8 2 7 2 5 0 13 2 1 0 17 3 5 0 12 3 4 2 1 2 0 0 18 2 1 0 17 3 2 0 is 3 2 2 13 2 0 0 18 3 0 0 17 3 0 0 17 3 0 2 is ~~ CompanySanitized. Does not contain TSCA CBI -- TTR TTT ---------- H`0-2R5a4i3n6b:oEwaTrrloyutL,ifOen-cSotragleynTcohxiucsimtyykiss DuPont 11953 -- `TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed ges Left Larvae Larvae 32 1A 32 24 32 1B 32 2B 33 1A 33 24 33 1B 33 2B 34 1A 34 2A 34 1B 34 2B ~~ 3s 1A 35 24 3s 1B 35 2B 36 1A 36 24 36 1B 36 2B 37 1A 37 24 37 1B 37 28 38 1A 38 24 38 1B 38 2B 39 1A 39 24 39 1B 39 2B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 [3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1811 3 0 0 17 3 0 0 171% 3 0 2 15M 2 0 0 181 3 0 0 17" 3 0 0 171% 3 0 2 151 2 0 0 18! 3 0 0 17" 3 0 0 172 3 0 2 15" 2 0 0 1811 3 0 1 16 3 0 0 ye 3 0 2 15% 2 0 1 17 3 0 1 16 3 0 0 171% 3 0 2 15 2 3 0 0 1 1 1167 3 0 0 172 3 0 2 15 2 0 1 7 3 0 1 16 3 0 0 on 3 0 2 15 2 0 1 17 3 0 1 16 3 0 0 171% 3 0 2 15 Bompany Sanitized. Does not contain TSCA CI Te tHo-2R5a4i3n6b:owEaTrrloyuLi,feOnSctoargheynTcohriucsimtyykiss DuPont11953 TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 40 1A 0 24 40 1B 40 2B 41 1A 41 24 41 1B 41 2B 42 1A 4 24 42 1B a 28 rm 43 1A 43 24 43 1B 43 28 44 1A "4 2a 44 1B 44 2B 45 1A 45 24 45 1B 45 28 46 1A 46 24 46 1B 46 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 [3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 17 3 0 1 16 3 0 0 1712 3 0 2 15% 2 0 1 17 3 0 1 16 3 0 0 172 3 0 2 154 2 0 1 [i 3 0 1 16 3 0 0 17s 3 0 2 15% 2 0 1 7 3 0 1 16 3 0 0 1712 3 0 2 157 2 0 1 17 3 0 1 16 3 0 0 172 3 0 2 15% 2 0 1 1 3 0 1 16 3 0 0 17 3 0 2 15 2 0 1 17 3 0 1 16 3 0 0 7" 3 0 2 1s a+ FScooulriorsiseincpludlpeesr:icocncucrevnaetdrtastpiieonne,iccor,ks1c4r.ew2t8)a.landbentspinal) ab PSmaaillnncosliozre (when compared o appropriste controls) --_ EompanySanitized. Doss not contain TSCA CBI - TTTe 205R4a3i6n:bowEaTrrloyuLt,ifOen-cSoirgheynTcohxiucsimtyykiss DuPon.11953 --~ TABLE 11 0.30 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Egg: Left Larvae Larva 0 1A 0 - 0 20 - . 0 24 0 - 0 20 - - 0 1B 0 - 0 20 - - 0 2B 0 - 0 20 - - 1 1A 0 - 0 20 - - 1 24 0 - 0 20 - - 1 1B 0 - 0 20 - - 1 2B 0 - 0 20 - - 2 1A 0 - 0 20 - - 2 24 0 - 0 20 - - 2 1B 0 - ~ 2 28 0 - 0 20 - - 0 20 - - 3 1A 0 - 0 20 - B 3 24 0 - 0 20 - B 3 1B 0 - 0 20 - - 3 28 0 - 4 1A 0 - 0 20 - - 0 20 - - 4 24 1 - 1 19 - - 4 1B 0 - 0 20 - - 4 28 0 - 5 1A 0 - 0 20 - 0 20 - - 5 24 0 - 5 1B 0 - 01 2109 -- -- 5 28 0 - 0 20 - - 6 1A 0 - 6 24 0 - 0 20 - - 1 19 - - 6 1B 0 - 0 20 - - 6 28 0 - 0 20 - - 7 1A 0 - 7 24 1 - 0 20 - - 2 18 - - 7 1B 0 - 0 20 - - 7 28 0 - 0 20 - : --_ ompany Sanltized. Doss not contain TSCA C81 - 110.R25a4i3n6b:oEwaTlroyuLt,ifOenScourgheynTcohsiucskmtyykiss Dupont11953 --~ TABLE 11 (Continued) 0.30 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Day Replicate! Removed Removed Dead Eggs Eggs Dead Left Larvae Live Larvae 8 1A 0 8 2A 0 8 1B 0 8 2B 0 9 1A 0 9 2A 0 9 1B 0 9 2B 0 10 1A 0 10 2A 0 10 1B 0 ~~ 10 2B 0 11 1A 0 1 2A 0 11 1B [J 11 2B 0 12 1A 0 12 2A 0 12 1B [J 12 2B 0 13 1A 0 13 2A 0 13 1B 0 13 2B I 14 1A 0 14 2A 0 14 4 B1B 00 15 1A 0 15 2A 0 15 1B 0 --_ 15 2B 0 0 20 = - - 2 18 - - - 0 20 " - - 0 20 - - - 0 20 - - - 2 18 - - - 0 20 - - - 0 20 - - - 0 20 - - - 2 18 - - - 0 20 - - - 0 20 - - - 0 20 - - - 2 18 - - - 0 20 - - - 0 20 - - - 0 20 - - - 2 18 - - - [J 20 - - - [J 20 - - - 0 20 - - - 2 18 - - - 0 20 - - - 1 19 - - - 0 20 - - - 2 18 - - -- 01 2109 -- -- - 0 20 - - - 2 18 - - - 0 20 - - - 1 19 - - BompanySanitized. Doss notcontain vena ney - LompanySanitized. Dossnotcontain TSCA CBI tHo2R5a4i3n6b:owETarrloyu,LiOfnecSourgheynTcohxiucsimtyykiss DuPont 11953 -- TABLE 11 (Continued) 030 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 16 1A 0 - 16 24 0 - 16 1B 0 - 16 28 0 - 17 1A 0 - 17 24 0 - 17 1B 0 - 17 28 0 - 18 1A 3 - 18 24 1 - 18 1B 3 - 18 28 1 - ~~ 9 1A 0 - 19 24 0 - 19 1B 0 - 19 28 0 - 20 1A 0 - 20 24 0 - 20 1B 0 - 20 28 0 - 21 1A 0 - 21 24 0 - 21 1B 0 - 21 2B 0 - 2 1A 0 - 2 24 0 - 2 1B 0 - 22 28 0 - 23 iA 0 - 23 24 0 - 23 1B 0 - 2 28 0 : 0 20 - - 2 18 - . 0 20 - - 1 19 - - 0 20 - - 2 18 - - 0 20 - - 1 19 - - 3 17 - - 3 17 - - 3 17 - - 2 18 - - 3 17 - - 3 17 - - 3 17 - - 2 18 - - 3 17 - - 3 17 - - 3 17 - - 2 18 - - 3 17 - - 3 17 - - 3 17 - - 2 18 - - 3 17 - - 3 17 - - 3 17 - - 2 Ji - - 3 17 - - 3 17 - - 3 17 - 3 2 18 - - --_--mn Bompany Seniized. Doesnot contain scacat ' H0R2aR5ai4i3nn6bb:oowwETaTrrrolouyutL,iOOfenn-ccSotorarhgeynnTccohhxuiucssimtpylyesss ______ DuDuepwointio11s953y PN TABLE 11 (Continued) 030 mg/L:TREOMUBTR,YOOncSorUhRyVnIchVuAsLmyAkNisDs,HEAATRCLHYILNIGFDE-ASTTAAGFEOTROTXHIECI9T0Y-TDEASYTRAINBOW Dead Eggs Dead Larvae Cumulative Live Cumulative Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 2 14 2 2a 0 0 - 2% 1B 0 - 4 2B 25 1A 0 0 0 23 2a 25 1B 0 0 0 0 25 28 2 1A 0 0 0 0 26 24 26 1B 0 0 0 0 ~ 26 2B 27 1A 0 0 0 0 27 24 0 0 2 1B 27 28 0 0 0 0 2 1A 0 0 28 2A 2 iB 0 0 0 0 2 28 2 1A 0 0 0 0 9 2A 2 iB 0 1 0 0 9 2B 30 1A 0 0 0 0 0 2A 30 1B 0 0 0 0 NE 0 0 31 iA 31 24 0 0 0 0 31 1B 31 28 0 0 0 0 3 17 - 3 17 - - 3 17 - - 2 18 - 3 17 0 0 3 17 0 0 3 17 0 0 2 18 0 0 3 17 0 3 17 0 0 0 3 17 0 0 2 18 0 3 1 0 0 6 3 12 0 5 3 16 0 2 17 0 1 i 3 4 0 13 3 3 0 3 13 0 14 4 2 14 0 4 3 1 0 16 3 4 1 5 0 0 16 i 2 3 3 0 0 0 is 17 3 0 0 17 4 2 3 0 1 0 13 17 3 0 0 17 3 4 0 1 0 0 17 1s 2 0 0 18 Company Sanitized. Does not contain Tana ~ay ee `BampanySe entzed. Doss not contain Ts caf 0H-2R5a4i3n6b:owEaTrroluytL,iOfnec-SoargheynTcohxiucsimtyykiss Dupon-11953 --_ TABLE 11 (Continued) 030 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 32 1A 32 24 0 0 0 0 32 1B 0 0 32 2B 0 0 33 1A 0 0 33 2A 0 0 33 1B 0 0 33 2B 0 0 34 1A 0 0 34 24 0 0 34 1B 0 0 34 2B 0 0 ~ 35 1A 0 0 35 24 0 0 3s 1B 0 0 3s 2B 0 0 36 1A 0 1 36 2A 0 0 36 1B 0 0 36 2B 0 0 37 1A 0 0 37 2A 0 0 37 1B 0 0 37 2B 0 0 38 1A 0 0 38 2A 0 0 38 1B 0 0 38 2B 0 0 39 1A 0 0 39 24 0 0 39 1B 0 0 39 2B 0 0 3 0 0 17 3 0 0 17 4 0 0 16% 2 0 0 18% 3 0 0 7 3 0 0 17" 4 0 0 16" 2 0 0 18% 3 0 0 17 3 0 0 17" 4 0 0 16 2 0 0 18% 3 0 0 17" 3 0 0 1" 4 0 0 16 2 0 0 18% 3 0 1 16" 3 0 0 17" 4 0 0 16" 2 0 0 18% 3 0 1 16 3 0 0 17" 4 0 0 16" 2 0 0 18% 3 0 1 16 3 0 0 nt 4 0 0 16! 2 0 0 18% 3 0 1 16" 3 0 0 7 4 0 0 16 2 0 0 18% PN CompanySanitized. Does not contain Tsca car ars 0H2R5a4i3n6b:owEaTrrloyutL,ifOen-cSoargheynTcohxiucsimtyykiss DuPont-11953 --~ TABLE 11 (Continued) 030 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FORTHE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 40 1A 0 0 40 2A 0 0 40 1B 0 0 40 2B 0 0 41 1A 0 0 41 2A 0 0 41 1B 0 0 41 2B a2 1A 0 0 0 1 4 2A 0 0 42 1B 0 0 --~ 42 28 0 0 43 1A 0 0 43 24 0 0 43 1B 0 0 43 2B 44 1A 0 0 0 0 44 2A 0 0 44 1B 0 0 44 2B 0 0 45 1A 0 0 45 24 0 1 45 1B 0 0 45 2B 0 0 46 1A 0 0 46 24 0 0 46 1B 0 0 46 28 0 0 3 0 1 16 3 0 0 17" 4 0 0 16" 2 0 0 18% 3 0 1 16" 3 0 0 17" 4 0 0 16 2 3 0 0 0 2 18% 15 3 0 0 Prd 4 0 0 16" 2 0 0 18% 3 0 2 is 3 0 0 171 4 0 0 16! 2 3 0 0 0 2 18% is 3 0 [J 7 4 0 0 16" 2 0 0 18% 3 0 2 1s 3 0 1 16" 4 0 0 16 2 0 0 18% 3 0 2 15 3 0 1 16 4 0 0 16" 2 0 0 18% a+ SFcooulriorseipsliicnactleusdpeesr:ccounrcveentdrsaptiinoen,(cior,ks1c4r.ew2tBi)l., and bent pineal) b Palein calor Eompany Senltized. Dossnot contain TSCA Ai a 01.2R5a4i3n6b:owEaTrrloyuLti,fOenScaogrheyTnonsuicsimtyykiss DuPon-11953 ~~ TABLE 12 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 0 1A 0 - 0 20 - - 0 24 0 - 0 20 - - 0 1B 0 - 0 2 - - 0 28 0 - 0 20 - - 1 1A 0 - 0 20 - - 1 24 0 - 0 20 - - 1 1B 0 - 0 20 - - 1 28 0 - 0 20 - - 2 1A 0 - 0 20 - - 2 24 0 - 0 20 - - 7 2 1B 0 - 0 20 - - 2 28 0 - 0 2 - - 3 1A 0 - 0 20 - - 3 24 0 - 0 20 - - 3 1B 0 - 0 20 - - 3 28 0 - 0 20 - - 4 1A 0 - 0 20 - - 4 24 0 - 0 20 - . 4 1B 0 - 0 20 - - 4 28 0 - 0 20 - - 5 1A 0 - 0 20 - - 5 24 0 - 0 20 - - 5 1B 0 - 0 2 - - 5 28 0 - 0 20 - - 6 1a 0 - 0 20 - - 6 24 1 - 1 19 - - |e 1B 0 - 0 20 - - 6 28 0 - 0 20 - - 7 1A 0 - 0 20 - - 7 24 0 - 1 19 - . 7 1B 0 - 0 20 - p ~~ 7 28 0 : 0 20 - - "Bompany Sanhized. Does rot contain TSCA CBr ae - 125R4a3in6b:owETlroyuLti,fOenScoargheynTcohxiucsimtyykiss -- TABLE 12 (Continued) DuPont 11953 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Day Replicate' Removed Removed Dead Egg: Eggs Dead Left Larvae Live Larvae 9 iA 0 - 0 20 - - 8 1A 0 - 8 2A 0 - 8 1B 0 - 8 2B 0 - 0 20 - - 1 19 - - 0 20 - - 0 20 - - 9 2A 0 - 1 19 - - 9 1B 0 - [J 20 - - 9 2B 0 - 0 20 - - 10 1A 0 - 0 20 - = 10 2A 0 - 1 19 - - ~ 100 218B 00 -- 11 1A 0 - 00 2200 -. -- 0 20 - - 11 2A 0 -. i 19 - - 11 1B 0 - 11 2B 0 - 12 1A 1 - 0 20 - - 0 20 - - 1 19 - - 12 2A [J - 12 1B 1 - 12 2B 0 - 1 19 - - 1 19 - - 0 20 - - 13 1A 0 - 13 2A 0 - 1 19 - - 1 19 - - 13 1B 0 - 13 2B 0 - 14 1A 1 - 14 2A 0 - | 14 1B 0 - 1I4s 21BA 00 -- 15 2A 0 - 15 1B 0 . --_ 15 2B 0 = 1 19 - - 0 20 - - 2 18 - - 1 19 - - 1 19 - - 20 2130 -- -- 1 19 - - 1 19 - - 0 20 - - _--_-- CompanySanitized. Does not contain TSCA C81 2to5R4a3i6nb:owBaTdrloyuLi,fOenScoargheynTcohruiscimyykiss DuPont-11953 --_ TABLE 12 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND TROUT, Oncorhynchus mykiss, HATCHING DATA FORTHE 90-DAY RAINBOW EARLY LIFE-STAGE TOXICITY TEST Dead Eggs Dead Cumulative Live Cumulative Larvae Dead Eggs Dead Live Day Replicate' Removed Removed ges Left Larvae Larvae 16 14 0 - 2 18 - - 16 24 0 - 16 1B 0 - 1 19 - - 1 19 - - 16 28 0 - 17 1A 0 - 0 20 - - 2 18 - - 17 24 0 - 17 1B 0 - 1 19 - - 1 19 - - 17 28 0 - 0 20 - - 18 1A 2 - 18 24 3 - 4 16 - 4 16 - - 18 1B 2 - 13 28 4 . 3 17 - - 4 16 - - ~ 19 1a 0 - 4 16 - - 19 24 0 19 1B 0 . 4 16 - - 3 17 - - 19 28 0 - 20 1A 0 - 4 16 - - 4 16 - - 20 24 0 - 20 1B 0 - 4 16 - - 3 17 - - 20 28 0 - 21 1A 0 - 4 16 - . 4 16 - - 21 2a 0 - 2 1B 0 - 4 16 - - 3 17 - - 21 28 0 - 2 1A 0 - 4 16 - - 4 16 - - 2 24 0 - 2 1B 0 - 4 16 - - 3 17 - - | 2 28 0 - 2 1a 0 - 4 16 - - 4 16 - - 2 24 0 - 23 1B 0 - 4 16 - - 3 17 - - 23 28 0 - 4 16 - : --~ BompanySanitized. Do note conts ainTscacsl --- Te ! l0 1Ho0.RR2aa5i4i3nn6bb:oowwETaTorrolouyutL,,iOfOnen-ccSootrragtleypnnTcochxhuiucssitmmyypkkisss DDuubPoonnti11s9s53y PN TABLE 12 (Continued) 0.63 mg/L:TREOMUBTR,YOOncSoUrhRyVnIchVuAsLmyAkNisDs,HEAATRCLHYILNIGFDE-ASTTAAGFEOTROTXHIECI9T0Y-DTAEYSTRAINBOW Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Egg: Left Larvae Larvae 24 1a 0 . 24 2a 24 1B 0 0 - 4 2B 0 . 5 1a 23 2a 0 0 0 0 25 1B 0 0 23 2B 6 1A 0 0 0 0 6 2A 2 1B 0 0 0 0 ~~ 2% 2B 27 1A 0 0 0 0 27 2a 27 1B 0 0 0 0 27 mB 0 0 8 1a 0 0 8 24 0 0 28 1B 8 2B 0 0 0 0 9 1A 0 0 9 2a 2 1B 0 0 0 0 9 2B 2 0 00 1a 30 2a 0 0 0 0 30 1B 1 0 0 2B 31 1A 0 0 0 0 31 2a 31 1B 0 0 0 0 31 28 0 0 -- 4 16 . - 4 16 - 3 17 - - 4 16 - - 4 16 0 4 16 0 0 0 3 17 0 0 4 16 0 4 1s 0 0 1 4 16 0 3 17 0 0 0 4 4 15 14 0 0 1 2 4 1s 0 3 1s 0 1 2 4 13 0 3 4 9 0 7 4 6 0 10 3 10 0 4 9 0 7 7 4 4 0 12 4 3 2 0 2 0 14 1s 6 3 0 1 4 2 4 0 0 0 14 16 4 0 0 16 6 4 2 0 0 0 12 16 4 0 4 0 0 0 16 16 6 0 0 14 EompanySanitized. Doesnotcontain sca car e er ms: L_ tHoo2RR5aa4ii3nn6bb:oowwETaTrrrolouyuLtti,fOOenncScoaorrglleyynnTccohhriuucssiomtpyylliissss DDuuoPmoniti11s95s3 `TABLE 12 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND TROUT, Oncorhynchus mykiss, HATCHING DATA FOR THE 90-DAY RAINBOW EARLY LIFE-STAGE TOXICITY TEST Dead Eggs Dead Cumulative Live Cumulative Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 32 1A 3 24 0 0 1 0 32 1B 0 0 32 2B 0 0 33 1A 33 24 0 0 0 0 33 1B 0 0 33 28 0 0 34 1A 0 0 34 24 0 0 34 1B 0 0 -- 34 2B 0 0 35 1A 35 24 0 0 0 0 35 1B 0 0 3s 2B 0 0 36 1A 36 24 0 0 0 0 36 1B 36 2B 0 0 0 1 37 1A 37 24 0 0 0 0 37 1B 37 28 0 0 0 0 38 1A 0 0 38 24 0 0 38 1B of 38 28 0 0 0 0 39 1A 39 24 0 0 0 0 39 1B 39 28 0 0 0 0 -- 4 4 0 0 1 0 15 16 4 0 0 16% 6 0 0 14" 4 0 1 15 4 0 0 16 4 0 0 16 6 0 0 14% 4 0 1 15% 4 0 0 16 4 0 0 16 6 0 0 14 4 4 0 0 1 0 15% 16 4 0 0 16% 6 0 0 14 4 0 1 15 4 0 0 16 4 6 0 0 0 1 16" 13 4 4 0 0 1 0 15 16 4 0 0 16" 6 0 1 13 4 0 1 15 4 0 0 16 4 6 0 0 0 1 16 13 4 4 0 0 1 0 15% 16 4 6 0 0 0 1 16" 13 {EampanySanitized. Doesnotcontain TSCA CB! TT -------- {H0-2R5a4i3n6b:owEaTrroluytL,iOfnec-oSargleynTcohxiucsitmyykiss Dupont-11953 --~ TABLE 12 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 40 1A 0 0 40 24 0 0 40 1B 0 0 4 2B 0 0 41 1A 0 0 41 24 0 0 41 1B 0 0 a 28 0 0 2 1A 0 0 2 24 0 0 2 1B 0 0 ~~ 2 2B 0 0 43 1A 0 0 a3 24 0 0 43 1B 0 0 43 2B 0 0 44 1A 0 0 "4 24 0 0 4" 1B 0 0 44 2B 0 0 45 1A 0 0 4 24 0 0 45 1B 0 0 45 2B 0 0 a6 1A 0 0 46 2A 0 0 46 1B 0 0 46 2B 0 0 4 0 1 15* 4 0 0 16 4 0 0 16" 6 0 1 13 4 0 1 15 4 0 0 16 4 0 0 16" 6 0 1 13 4 0 1 15 4 0 0 16 4 0 0 16 6 0 1 13" 4 0 1 15 4 0 0 16 4 0 0 16 6 0 1 3 4 0 1 15% 4 0 0 16 4 0 0 16 6 0 1 3 4 0 1 15 4 0 0 16 4 0 0 16 6 0 1 3 4 0 1 15* 4 0 0 16% 4 0 0 16 6 0 1 13" a+ FSocuolrirosies inpclupldeesr:iccouncrcevneatdrasttpiionnee,(csior.ks1c4re-w2t8a)l.,and bent spina) bdd LPoaslseoifnceoqluoirlibrium -- Company Saniized. Does not contain TSCA car -------- 1HA2R5a4i3n6b:owEaTrroluyLti,fOenScoargheynTcohxiucsimtyykiss DuPont-11953 TABLE 13 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATAFORTHE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 0 1a 0 - 0 20 - . 0 24 0 - 0 20 - x 0 1B 0 - 0 20 - - 0 2B 0 - 0 20 - - 1 1A 0 - 0 2 - - 1 24 0 - 0 2 - - 1 1B 0 - 0 20 - - 1 28 0 - 0 20 - - 2 1A 0 - 2 24 0 - 0 20 - - 0 20 - - --_ 2 1B 0 - 0 20 - - 2 2B 0 - 0 20 - - 3 1A 0 - 0 20 - - 3 24 0 - 0 20 - - 3 1B 0 - 0 20 - - 3 28 0 4 1A 0 - 0 20 - - 0 20 - - 4 24 0 - 0 20 - - 4 1B 0 - 0 20 - - 4 2B 0 - 0 20 - - 5 1A 0 - 0 20 - - 5 24 0 - 0 20 - - 5 1B 0 - 0 2 - - 5 28 0 - 6 1A 0 - 0 20 - - 0 20 - - 6 24 0 - 0 20 - - 6 1B 0 6 2B 0 - 0 20 - - 0 20 - - 7 1A 0 - 7 24 0 - 0 20 - - 0 2 - - 7 1B 0 - -- 7 28 0 - 0 20 - - 0 20 - - - `Company Sanitized. Doos notcantain Tera ray ZtFoo-R2Ra5ai4in3n6bb:oowwBTTarrrooyuwLti,OfOnencScotorargbheyynnTcochxhuiucsismtmyypkibslss --~ DDuuPbonto.1i195s3 `TABLE 13 (Continued) 1.3 mg/L: TERMOUBTR,YOOncSoUrRhyVnIcVhAusLmAykNisDs,HEAATRCLHYINLGIFDEA-TSTAAFGEORTOTXHIECI90T-YDTAEYSRTAINBOW Dead Eggs Dead Cumulative Live Cumulative Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 8 1A 0 - 8 24 0 - 8 1B 0 - 8 28 0 - 9 1A 0 - 9 24 0 - 9 1B 0 - 9 28 0 - 10 1A 0 - 10 24 0 - ~ 10 1B 10 28 0 0 2 - 1 1A 0 - 1 24 0 - in 1B 0 - 11 28 0 - 12 ia 0 . 12 24 2 - 12 1B 0 - 12 2B 0 13 1A 0 - 13 24 0 - 13 1B 0 - 3 28 0 - 14 iA 0 - 14 24 0 - |e 1B 1 - 14 28 0 - 1s iA 0 . 1s 24 0 - is iB 0 - -- 1s 28 0 - 0 2 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 2 18 - - 0 20 - - 0 20 - - 0 20 - - 2 18 - - 0 20 - - 0 20 - - 0 2 - - 2 13 . - 1 19 - - 0 20 - - 0 20 . - 2 13 - - 1 19 - - 0 20 - - Company Sanitized. Doesnotcontain Tso can = o{HR02aR5ia4in3n6bb:oowwETaTrrrolouyuLt,:iOfOnencScoosrrgbheyynnTccohhruuisscmimpyykhiissss -- bDwuoPownti-1o19s5s3 TABLE 13 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAYRAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Day Eggs Larvae Replicate! Removed Removed Dead Eggs Dead Eggs Left Larvae Live Larvae 16 1A 0 - 0 20 - - 16 2A 0 - 2 18 - s 16 1B 0 - 1 19 - - 16 2B [J - [J 20 - - 17 1A 0 - 0 20 - - 1177 21AB 00 -- 17 2B 0 - 21 1198 -- -- 0 20 - - 18 1A 3 - 3 17 - - 18 2A 1 - 3 17 - - 18 1B _ 18 8 2 2 - - 3 17 - 2 18 - - - 1199 21aA 00 -- 33 1177 -- .- 1199 o1mB 00 -- 23 1187 .- -- 20 1A 0 - 3 17 - - 20 2A 0 - 3 17 - - 20 1B 0 - 3 17 - - 20 2B 0 - 2 18 - - 21 1A 0 - 3 17 - - 21 2A 0 - 3 17 - - 21 1B 0 - 3 17 - - 21 2B 0 - 2 18 - - 22 1A 0 - 3 17 - - 22 2A 0 - 3 17 - - 22 1B 0 - 3 17 - - 32 21BA 00 -- 32 1718 -- -- 23 2A 0 - 3 17 - - 23 1B 0 - 3 17 - - 23 2B 0 - 2 18 - - ~ Company Sanitized. Doss not contain TSCA cay aa 1.R2a5i4n36b:oEwaTrrloyuL,ifOenSctoargleynTcoxhiucsitmyykiss Dupont 11953 --~ TABLE 13 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Egg Left Larvae Larvae 24 1A 24 2A 2 1B 24 2B 25 1A 25 2A 25 1B 25 2B 26 1A 26 2A 26 1B ~~. 26 2B 27 1A 27 2A 27 1B 27 2B 28 1A 28 2A 28 1B 28 2B 29 1A 29 2A 29 1B 29 2B 30 1A 30 2A 30 1B | 30 2B 31 1A 31 2A 31 1B 31 2B -- -- 0 - 0 - 0 - 0 - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 [J 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 17 - - 3 17 - = 3 17 - - 2 18 = - 3 17 0 0 3 16 0 1 3 17 0 0 2 18 0 0 3 17 [J 0 3 16 0 1 3 16 0 1 2 18 0 0 3 15 0 2 3 15 0 2 3 16 [J 1 2 16 0 2 4 10 0 6 3 11 0 6 3 12 0 5 2 12 0 6 4 2 0 14 3 3 0 14 3 1 0 16 2 6 0 12 4 1 0 15 3 2 0 15 3 0 0 17 2 3 0 15 4 0 0 16 3 1 0 16 3 0 0 17 2 0 0 18 Company Sanitized. Doesnot contain Tara ner = PAompany. `Saniized. Dossnotcontain Tc on H{0-2R5a4i3n6b:owEaTrrloyu,LiOfnec-oSargheynTcohriucsimtyykiss --_ `TABLE 13 (Continued) DuPont. 11953 1.3 mg/L: TERMOUBTR,YOOncSoUrRhyVnIcVhAusLmAykNisDs,HEAATRCLHYINLGIFDEA-TSTAAFGOERTTOHXEIC9I0T-YDTAEYSRTAINBOW Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 32 1A 0 0 32 2A 0 0 32 1B 0 0 32 2B 0 0 33 1A 0 0 33 2A 0 0 33 1B 0 0 33 2B 0 0 34 1A 0 0 34 24 0 0 34 1B 0 0 --~ 34 2B 0 0 35 1A 0 1 35 24 0 0 35 1B 0 0 35 28 0 2 36 1A 0 0 36 24 0 0 36 1B 0 0 36 28 0 0 37 1A 0 0 37 24 0 0 37 1B 37 28 0 0 0 0 38 1A 0 0 38 24 0 0 38 1B 0 0 | 3 2B 0 0 39 1A 0 0 39 24 0 0 39 1B 0 0 39 28 0 0 --_ 4 0 0 16 3 0 0 7 3 0 0 1" 2 0 0 18% 4 0 0 16 3 0 0 jo 3 0 0 17 2 0 0 18% 4 0 0 16 3 0 0 7 3 0 0 17 2 4 0 0 [J 1 1185% 3 0 0 17 3 0 0 17 2 0 2 16 4 0 1 1s 3 0 0 17 3 0 0 17 2 0 2 16 4 0 1 1s 3 0 0 17 3 2 0 0 0 2 7 16 4 0 1 15 3 0 0 17 3 0 0 7 2 0 2 16 4 0 1 15 3 0 0 17 3 0 0 Jo 2 0 2 16 Company Sanitizec. Does not contain TSA ani EE tHo2R5a4i3n6b:owEaTrrloyuL,ifOen-cSotrahgeynTcohxuicsitmyykiss DuPont-11953 --~ TABLE 13 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvac Larvae 40 1A 0 0 4 24 0 0 4 0 1 1s 3 0 0 17 40 1B 0 0 40 28 0 0 3 0 0 17 2 0 2 16 a 1A 0 0 a 24 0 0 4 0 1 1s 3 0 0 17 41 1B 0 0 41 2B 0 0 3 0 0 17 2 0 2 16 2 1A 0 0 4 0 1 1s a2 24 0 0 3 0 0 7 a2 1B 0 0 2 2B 0 0 3 0 [J 7 2 0 2 16 rm 4 1A 0 0 4 0 1 is 43 EIN 0 0 3 0 0 17" 43 1B 0 0 a 28 0 0 3 0 0 17 2 0 2 16 4" iA 0 0 4 0 1 1s 44 24 0 0 3 0 0 7 44 1B 0 0 44 28 0 0 3 0 0 7 2 0 2 16 45 1A 0 0 4 0 1 1s 45 2A 0 0 3 0 0 7 45 1B 0 0 45 2B 0 0 3 0 0 ww 2 0 2 16 46 1A 0 0 4 0 1 15% 46 2A 4 1B 0 0 0 0 3 3 0 0 0 0 17 17 46 28 0 0 2 0 2 16 a+ FSocuolriorespilsic(aitncelsupdeesr:ccournvecd espinnet,(criocra.k1stcArie-w2o8tn)ai.l and beat spinclail) b SPmaallelinincosliozre (when compared to appropriate controls) ~ BompanySanitized.Does notcontainTSCACBI es -- 1102R5a4i3n6:bowEaTrrloyuL,iOfnecSourgheynTcohxuisctmyykiss -- TABLE 14 Dupont-11953 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 0 1A [ - 0 20 - - 0 2A 0 - [J 20 - - 00 218B 00 -. 00 2200 -- -- 1 1A 0 - 1 2A 0 - 0 20 - - 0 20 - - 1 1B 0 - 0 20 - - 1 2B 0 - 2 1A 0 - 2 2A 0 - ~ 2 1B 0 - 23 12AB 00 -- 0 20 - - 0 20 - - 0 20 - - 0 20 - - 00 2200 -- -- 3 2A 0 - 3 1B 0 - 3 2B 0 - 0 20 - - 0 20 - - 0 20 - - 4 1A 0 - 4 2A 0 - 4 1B 0 - 0 20 - - 0 20 - - 0 20 - - 4 2B 0 - 55 214A 00 -- 0 20 - - 00 2200 -. -- 5 1B 0 - [J 20 - - s 2B 0 - 0 20 - - 6 1A 0 - 0 20 - - 7 1A 2 - 2 13 - - 6 2A 0 - 0 20 - - 6 1B 0 - 0 20 - - 6 2B 0 - [J 20 - - 7 2A 1 - 7 1B 0 - 1 2B 0 - 1 19 - - 0 20 - - 0 20 = - BompanySanitized. Does not contain TSG 8 Te H:2R5a4i3n6b:owBTarroyutL,ifOen-cSotrahgeynTcohxuisctmyykiss DuPont-11953 --~ TABLE 14 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 8 1A 0 - 3 24 0 - 8 1B 0 - 8 28 0 - 9 14 0 - 9 24 0 . 9 1B 0 - 9 2B 0 - 10 1A 0 x 10 24 0 - a. 10 1B 0 - 10 28 0 - 1 1A 0 - 1 24 0 - il 1B 0 - 1 28 0 - 12 1A 0 - 12 24 1 - 12 1B 0 - 12 28 0 - 3 1A 0 - 13 24 0 - 13 1B 0 - 13 28 1 - 14 1A 0 - 14 24 0 - |e 1B 1 - 14 2B 0 - 1s 1A 0 - 1s 24 0 - is 1B 0 - 1s 28 0 z 2 18 - - 1 19 - - 0 20 - - 0 20 - - 2 18 - - 1 19 - - 0 20 - - 0 20 - - 2 18 - - 1 19 - - 0 2 - - 0 20 - - 2 18 - - 1 19 - - 0 20 - - 0 20 - - 2 18 - - 2 18 - - 0 2 - - 0 20 - - 2 18 - - 2 18 - - 0 20 - - 1 19 - - 2 18 - - 2 18 - - 1 9 - - 1 19 - - 2 18 - - 2 18 - - 1 19 - - 1 19 - - Company Sanitized. Dossnotcontain Tach may Re: Lt2oo5RR4aa3ii6nnb:boowwETaTrrrolouyut,L,iOOfnenc-cSootrrabhgeyynnTccohxhuiucssimtyypkliiss __ DDuubPoonntd11i9o5s3y TABLE 14 (Continued) 2.5 mg/L: TERMOUBTR,YOOncSoUrRhyVnIcVhAusLmAykNisDs,HEAATRCLHYINLGIFDEA-TSTAAFGEORTOTXHIECI9T0Y-DTAEYSTRAINBOW Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eges Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 16 1A 0 - 16 24 0 - 16 1B 0 - 16 28 0 - 17 1A 0 - 17 24 0 - 17 1B 0 - 17 28 0 - 18 1A 2 - 18 24 2 - 18 1B 3 - -- 18 2B 1 - 19 1A 0 - 19 24 1 - 19 iB 0 - 19 2B 0 - 20 1A 0 - 20 24 0 - 2 1B 0 - 2 28 0 - 21 1A 0 - 21 24 0 - 21 1B 0 - 2 28 0 - 2 1A 0 - 2 24 0 - 2 iB 0 - | 2 28 0 - 2 1A 0 - 2 24 0 - 23 1B 0 - 2 28 0 - --~ 2 18 - - 2 18 - - 1 19 - - 1 19 - - 2 18 - - 2 18 - - 1 19 - - 1 19 - - 4 16 - - 4 16 - - 4 16 - - 2 18 - - 4 16 - - 5 15 - - 4 16 - - 2 18 - - 4 16 - - 5 15 - - 4 16 - - 2 18 - - 4 16 - - 5 15 - - 4 16 - - 2 18 - - 4 16 . - 5 15 - - 4 16 - - 2 18 - - 4 16 - - 5 1s - - 4 16 - - 2 18 - - `Company Sanitized. Doan natentain Tra 1 -- H102RR5ae4ii3nn6bb;oowwBTaTrrrlooyuutL,,iOOfnnecScotorarbgheyynnTccohhxuiucssimmtbyykiiesss PN DDuuopomnil-1i19s53y TABLE 14 (Continued) 25 mg/L: TERMOUBTR,YOOncSoUrRhyVnIcVhuAsLmAykNisDs,HEAATRCLHYINLGIFDEA-TSTAAFGEORTOTXHIECI9T0Y-DTAEYSTRAINBOW Dead Eggs Dead Cumulative Live Cumulative Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 2 1A 0 - 2 24 0 - 2 1B 2 28 0 0 - 25 1A 0 0 25 24 0 0 25 1B 0 0 25 28 0 0 26 1A 0 0 26 24 0 0 26 1B ~ 26 28 0 0 0 0 27 1A 1 0 27 24 0 0 27 1B 0 0 27 28 23 1A 0 0 0 0 28 24 23 1B 0 0 0 0 28 2B 0 0 29 1A 2 0 2 24 0 0 29 1B 0 0 29 28 30 1A 0 0 0 0 30 24 0 0 30 1B 0 0 | 30 28 0 0 31 1A 0 0 31 24 31 1B 0 0 0 0 31 28 0 0 PN 4 16 - - 5 1s - - 4 16 - - 2 18 - 4 16 0 0 5 1s 0 0 4 16 0 0 2 18 0 0 4 16 0 0 5 1s 0 0 4 16 0 0 2 13 0 5 13 0 0 2 5 15 0 0 4 9 0 7 2 16 0 2 5 10 0 5 5 13 0 2 4 5 0 1 2 13 0 5 7 4 0 9 5 10 0 5 4 1 0 1s 2 5 0 13 7 2 0 n 5 4 0 1 4 0 0 16 2 3 0 15 7 2 0 1 5 3 0 4 0 0 12 16 2 2 0 16 - `Company Santizes boos not contain TSCA cn: S1oH0R2Ra5ai4in3nb6bo;onEwaTTrrrlooyuuLt,i,OfOenn-ccSootrrahgheyynnTcocxhhiuucsistomyybklissss -- DuDuoPnontt-l1919s53s `TABLE 14 (Continued) 2.5 mg/L: TERMOBUTR,YOOncSoUrRhyVnIcVhuAsLmAykNisDs,HEAATRCLHYINLGIFDEA-TSTAAFGEORTOTXHIECI9T0Y-DTAEYSTRAINBOW Dead Eggs Dead Cumulative Live Cumulative Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 32 1A 0 0 32 24 2 0 32 1B 0 0 32 28 1 0 3 1A 0 0 3 24 0 0 33 1B 0 0 33 2B 34 1A 0 0 0 0 34 24 34 1B 0 0 0 0 --~ 34 2B 35 1A 0 0 0 0 35 2A 35 1B 0 0 0 0 35 2B 36 1A 0 0 0 0 36 2A 36 1B 0 0 0 0 36 2B 37 1A 0 0 0 0 37 2A 37 1B 0 0 0 0 37 2B 38 1A 0 0 0 0 38 2A 0 0 38 1B 0 0 38 2B 0 0 39 iA 0 0 39 2A 0 0 39 1B 0 0 39 2B 0 [] 7 1 0 12 7 0 0 13 4 0 0 16 3 0 0 17 7 0 0 13 7 0 0 13 4 0 0 16 3 7 0 0 0 17 0 13 7 4 0 0 0 0 13" 16 3 7 0 0 0 0 ir 13 7 4 0 0 0 0 13" 16 3 0 0 ir 7 0 0 13 7 0 0 13 4 0 0 16 3 0 0 17% 7 0 0 13 7 4 0 0 0 0 13" 16 3 0 0 17 7 0 0 13 7 0 0 13 4 0 0 16" 3 7 0 0 0 0 17% 13 7 0 0 13 4 0 0 16 3 0 0 17TM --~ Company Saniized. Dossnotcontain Tc cy _-- A --m-- -- m- r 1H0R-2Ra5ai4in3nb6b:ooEwwaTrTrrlooyuuLt,,ifOOenn-ccSootrraghleyynnTcocxhhiucusistmmyypkliissss -- TABLE 14 (Continued) DuDbuoPwoniti1o19s53y 2.5 mg/L:TERMOUBTR,YOOncSoUrRhyVnIcVhuAsLmAykNisDs,HEAATRCLHYINLGIFDEA-TSTAAFGEORTOTXHIECI9T0Y-DTAEYSTRAINBOW Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 40 1A 0 0 40 2A 40 1B 0 0 0 1 40 2B 0 0 4 1A 0 0 41 2A 0 0 4 1B 0 0 41 28 2 1A 0 0 0 0 42 24 0 0 2 1B 0 0 ~ 4s 2 a28 00 00 43 2A 43 1B 0 0 0 0 43 2B 0 0 44 1A 0 0 44 2A 44 1B 0 0 0 0 " 28 0 1 45 1A 0 0 45 24 0 0 4 1B 0 0 4s 28 0 1 46 1a 0 0 46 2A 46 1B 0 0 0 0 "|L46 28 0 0 7 0 0 13 7 4 0 0 0 1 13" 15 3 0 0 ot 7 0 0 13 7 0 0 12 4 0 1 1s 3 0 0 wi 7 0 0 13 7 0 0 12 4 0 1 Is 73 00 00 1713 7 4 0 0 0 1 12" 1s 3 0 0 17 7 0 0 13 7 0 0 12" 4 0 1 15 3 0 1 16 7 0 0 13 7 0 0 12 4 0 1 1s 3 0 1 16 7 0 0 13 7 4 0 0 0 1 12" 1s 3 0 1 16 a1 ScFooluirorsise(ipncllupdeeisr:cccounarcveetndtesrpiasnte,ioconr,ks1c4rew2t8a)i.l, and bent spinetail) b SPmaallelinincosilzoer (when compare to spproprise control(9) Pe * One larvae missing, possibly escaped from embryo cup and went down sandpipe dain. Company Sanitzas. Does not coma om ee etme SSERAAR0, tHo2R5a4i3n6b:owEaTrrloyutL,iOfnecSotargheynTcohxiucsimtyykiss Dupont 11953 -- TABLE 15 5.0mgL: TERMOUBTR,YOOncSoUrRhyVnIcVhuAsLmAykNisDs,HEAATRCLHYINLGIFDEA-TSTAAFGOERTOTXHIEC9I0T-YDTAEYSRTAINBOW Dead Eggs Dead Cumulative Live Cumulative Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 0 1A 0 - 0 24 0 - 0 20 . - 0 20 - - 0 1B 0 - 0 28 0 - 0 20 - - 0 20 - - 1 1A 1 24 0 0 - 00 2200 -- -- 1 1B 0 - 1 28 0 - 0 20 . - 0 20 - - 2 1A 0 - 2 24 0 - 0 20 - - 0 20 - - ~~ 2 1B 0 2 28 0 - 0 0 2200 -- -. 3 1A 0 - 3 24 0 - 0 20 - - 0 20 - - 3 1B 0 - 3 28 0 - 0 20 - . 0 20 - - 4 1A 0 - 4 24 0 - 0 20 0 20 - - 4 1B 0 - 0 20 - - 4 28 0 - 5 1A 0 - 0 20 0 20 - - 5 24 0 - 5 1B 0 - 0 20 - - 0 20 - - 5 28 0 - 6 1A 0 - 0 20 - . 0 20 - - 6 24 6 1B 0 0 - 0 0 2200 -- -: 6 28 0 - 7 1A 0 - 0 20 - - 0 20 - - 7 24 0 - 7 1B 0 - 0 20 - - 0 20 - . -- 7 28 0 - 0 20 - - "s- comeeTM gente aoe" 025R4a3in6b:owEaTrroluyLti,fOenScaorgheynTcohruiscimyykiss Dupont11953 --~ TABLE 15 (Continued) 5.0 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 8 14 0 - 8 24 0 - 8 1B 0 - 8 28 0 - 9 1A 0 - 9 24 0 - 9 1B 0 - 9 28 0 - 10 1A 0 - 10 24 0 - --~ 10 1B 0 - 10 28 0 - 1 1A 0 - 1 24 0 - 1 1B 0 - 11 28 0 - 12 1A 0 - 12 24 0 - 12 1B 0 - 12 28 0 - 13 1A 0 - 13 24 0 - 13 1B 0 - 13 28 0 - 14 1A 0 - 14 24 0 - Cla 1B 0 - 14 28 0 - 1s 1A 0 - 1s 24 0 - 15 1B 1 - ~~ 15 28 0 - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 . - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 0 20 - - 1 19 - - 0 2 : - ---- yee , DposEsotoontan o 2to5R4a3i6nb:oEwaTrrloyuL,ifOen-cSoargheynTcoxhiucsitmyykiss DuPont-11953 -- `TABLE 15 (Continued) 5.0mg/L: EMBRYO SURVIVAL AND HATCHING DATA FORTHE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left larvae Larvae 16 1a 0 - 16 24 0 - 16 1B 0 - 16 28 0 - Id 1A 0 - 17 24 0 - 17 1B 0 - 17 28 0 - 18 1A 3 - 18 24 2 - --~ 18 1B 1 18 28 2 - 19 1A 0 - 19 24 0 - 19 1B 0 - 19 2B 0 - 20 1A 0 - 20 24 0 - 20 1B 0 - 20 2B 21 1A 0 0 - 21 24 0 - 21 1B 0 - 21 28 0 - 2 14 0 - 2 24 0 - 2 1B 0 - 2 28 0 - 2 iA 0 - 23 24 0 - 2 1B 0 - --_ 2 28 0 - 0 20 - - 0 20 - - 1 19 - . 0 20 - - 0 20 - - 0 20 - - 1 9 - - 0 20 - - 3 17 - - 2 18 - - 2 18 - - 2 13 - - 3 17 - - 2 13 - - 2 18 - - 2 18 - - 3 17 - - 2 18 - - 2 18 - - 2 3 18 17 -- -- 2 18 - - 2 18 - - 2 18 - - 3 17 - - 2 18 - - 2 18 - - 2 18 - - 3 17 - - 2 18 - - 2 18 - - 2 18 - - CompanySanitized. Does not contain TSCA ~a1 = -_-- oH25R4a3i6n:boEwaTrrloyuLti,fOenScaorgheynTcohiucsitmyys Dupont 11953 --TABLE 15 (Continued) 5.0 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eges Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 2 1A 0 - 2 24 0 - 2 1B 0 - 2 28 0 - 25 1A 0 0 25 24 0 0 25 1B 0 0 25 28 1 0 26 1A 0 0 26 24 0 0 --_ 26 1B 0 0 26 2B 0 0 27 1A 0 0 27 24 0 0 27 1B 0 0 27 2B 0 0 2 1A 0 0 23 24 0 0 28 1B 0 0 28 28 0 0 29 1A 0 0 29 24 0 0 2 1B 0 0 2 2B 0 0 30 1A 0 0 30 24 0 0 30 1B 0 0 30 2B 1 0 31 1A 0 0 al 24 0 0 31 1B 0 0 --~ 31 28 0 0 3 17 - - 2 18 - - 2 18 - - 2 18 - - 3 17 0 0 2 17 0 1 2 18 0 0 3 17 0 0 3 15 0 2 2 17 0 1 2 18 0 0 3 17 0 0 3 13 0 4 2 15 0 3 2 13 0 0 3 17 0 0 3 5 0 2 2 2 0 6 2 is 0 3 3 13 0 4 3 1 0 16 2 6 0 12 2 10 0 8 3 6 0 1 3 0 0 17 2 3 0 15 2 3 0 15 4 5 0 1 3 0 0 17 2 1 0 17 2 1 0 17 4 1 0 1s [Company Sanitized. Doss not contain TSA ARI EE 110.2R5a4i3n6b:oEwaTrrloyuLi,fOenScaorgheyTnochruiscimyykiss DuPont11953 --~ TABLE 15 (Continued) 5.0 mg/L: EMBRYO SURVIVALAND HATCHING DATA FORTHE90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 32 1A 0 0 32 2A 0 0 32 1B 0 0 32 2B 0 0 33 1A 0 0 33 2A 1 0 33 1B 0 0 33 28 0 0 34 1A 0 0 34 2A 0 0 ~~ 34 1B 0 0 34 28 0 0 35 1A 0 0 El 2A 0 0 35 1B 0 0 35 28 0 0 36 1A 0 0 36 24 0 0 36 1B 0 0 36 28 0 0 37 1A 0 0 37 2A 0 0 37 1B 0 0 37 28 0 0 38 1A 0 0 38 2A 0 0 38 1B 0 0 38 28 0 0 39 1a 0 0 39 2A 0 1 39 1B 0 0 --~ 39 28 0 0 3 0 0 17 2 1 0 17 2 0 0 18 4 0 0 16 3 0 0 17 3 0 0 17 2 0 0 18" 4 0 0 16 3 0 0 17 3 0 0 17 2 0 0 18" 4 0 0 16 3 0 0 17 3 0 0 17" 2 0 0 18" 4 0 0 16 3 0 0 17 3 0 0 17 2 0 0 18" 4 0 0 16 3 0 0 17 3 0 0 7 2 0 0 18 4 0 0 16 3 0 0 17 3 0 0 17 2 0 0 18" 4 0 0 16 3 0 0 17 3 0 1 16" 2 0 0 18" 4 0 0 16 [Company Senitized. Do noe conts ain TSCA C5: Te {25R4a3i6n:boEwaTtroyuLti,fOen-SctoargkeynTcoshiucsimtyykiss DuPont11953 -- TABLE 15 (Continued) 5.0mg/L: EMBRYO SURVIVAL AND HATCHING DATA FORTHE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Eggs Dead Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 40 1A 0 0 40 2A 0 0 40 1B 0 0 40 28 0 0 a 1A 0 0 41 2A 0 0 41 1B 0 0 a 28 0 0 42 1A 0 0 42 2A 0 0 ~ 42 1B 2 28 0 0 0 0 a 1A 0 0 43 EIN 0 0 43 1B 0 0 43 28 0 0 44 1A 0 0 44 2A 0 0 44 1B 0 0 44 28 0 0 45 1A 0 0 45 2A 0 0 45 1B 0 0 45 28 0 0 46 1A 0 0 46 24 0 0 46 1B 0 0 46 28 0 0 3 0 0 17 3 0 1 16" 2 0 0 18" 4 0 0 16 3 0 0 17 3 0 1 16" 2 0 0 18% 4 0 0 16 3 0 0 17 3 0 1 16* 2 0 0 18% 4 0 0 16 3 0 0 17 3 0 1 16* 2 0 0 18% 4 0 0 16 3 0 0 17 3 0 1 162 2 0 0 18% 4 0 0 16 3 0 0 17 3 0 1 162 2 0 0 18* 4 0 0 16 3 0 0 17 3 0 1 16* 2 0 0 18% 4 0 0 16 a+ SFcooulriorseipsliicnactleusdpeesr:ccounrcveentdrsaptiionn,iccor,ks1c4r.e2w8t)a.il, and bet spin/tail) ~~ `Company Sanitized. Does not contain TS rer 86- - TABLE 16 DILUTION WATER CONTROL: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Day Replicate Live Larvae Swimming Up 40 1A 15 3 40 2A 15 2 40 1B 17 3 40 2B 15 4 41 1A 15 3 41 2A 15 4 41 1B 17 4 41 2B 15 4 42 1A 15 3 2 2A 15 4 a2 1B 17 4 42 2B 15 4 ~~ 43 1A 15 4 43 2A 15 5 43 1B 17 6 43 2B 15 6 44 1A 15 5 44 . 2A 15 7 44 1B 17 6 4 2B 15 7 45 1A 15 6 45 2A 15 8 45 1B 17 9 45 2B 15 11 46 1A 15 7 46 2A 15 8 46 1B 17 9 46 2B 15 11 - ---- 1F0H2R5a4i3n6b:owEaTrrloyLui,fOenScourgheynTcohxuicsimtyykiss DuPon-11953 TABLE 17 IPA CONTROL: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 17 0 40 24 16 3 40 1B 17 1 40 28 1s 4 4 1A 17 3 a 2a 16 3 41 1B 17 3 41 28 1s 4 42 1A 17 4 42 24 16 6 a 1B 17 3 42 28 1s 5 ~ 43 1A 17 4 43 24 16 6 43 1B 17 4 43 28 15 5 44 1A 17 5 44 2A 16 9 44 1B 17 5 44 28 15 5 45 1A 17 8 45 24 16 9 45 1B 17 8 45 2B 15 10 46 1A 17 8 46 24 16 9 46 1B 17 10 46 28 15 10 ~~ nySanta.Dove conan TSCA C2! re B025R4a3i6n:bowEaTrrloyuL,ifOSnagce oTorsmiytiss ~ TABLE 18+ Dupo 11953 0.30 mg/L: SWIM-UP DATA FORTHE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Toul Tol Larvae D: Replicate Live Larvae Swimming Up 40 1A 16 6 40 2A 17 5 40 1B 16 3 40 2B 18 1 41 1A 16 4 41 2A 17 4 41 1B 16 3 41 2B 18 4 42 1A 15 5 42 2A 17 3 42 1B 16 3 42 2B 18 4 ~~ 43 1A 15 5 43 2A 17 4 43 1B 16 3 43 2B 18 5 44 1A 15 4 44 2A 17 3 44 1B 16 6 44 2B 18 5 45 1A 15 8 45 2A 16 10 45 1B 16 1 45 2B 18 11 46 1A 15 9 46 2A 16 10 46 1B 16 1m 46 2B 18 1 --~ gin anaes Doss conan TSCACE! EE Lom to Rainbow Trout, Oncorhynchus mykiss DuPont-11953 TABLE 19 0.63 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Day Replicate Live Larvae Swimming Up 40 1A 15 4 40 2A 16 1 40 1B 16 2 40 2B 13 4 41 1A 15 5 41 2A 16 2 41 1B 16 5 41 2B 13 4 42 1A 15 4 42 2A 16 3 4 1B 16 6 42 2B 13 4 ~ 43 1A 15 5 43 2A 16 4 43 1B 16 6 43 2B 13 5 44 1A 15 7 44 2A 16 6 4 1B 16 6 44 2B 13 7 45 1A 15 11 45 2A 16 8 45 1B 16 8 45 2B 13 8 46 1A 15 11 46 2A 16 8 46 1B 16 1n 46 2B 13 10 rr `Company Senitized, Doseot containTSCACB! +Ho:2R5a4i3n6b;owEaTrroluyLti,fOencSotragheynTcohxiucsimtyykiss Dupon-11953 ~ TABLE 20 1.3 mg/L: SWIM-UP DATA FOR EARLY THE 90-DAY LIFE-STAGE RAINBOW TOXICITY TTERSOTUT, Oncorhynchus mykiss, Day Replicate Liv"eToLtaarlvae Total Larvae Swimming Up 40 40 1A 24 1s 17 1 1 40 40 1B 28 17 16 1 0 41 41 iA 24 15 17 3 3 41 41 1B 17 28 16 4 2 42 1A 1s 42 24 17 4 6 a a 1B 28 7 16 7 5 -- 43 ia 43 24 15 i 4 6 4 4 1B 28 7 16 7 6 4 44 1A 24 1s 17 5 6 44 44 1B 17 28 16 8 1 45 45 iA 24 is 17 8 6 4455 218B 17 16 n 10 46 1A is 46 24 17 8 9 46 46 1B 28 17 16 1 u Company SanDoiestnoticonztaineTSdCAC.BI - 1F0-R2a5i43n6b;owEaTrrloyutL,ifOencSotrahgeynTcohxuicsimtyykiss --_ DuPont-11953 TABLE 21 2.5 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 13 0 40 24 13 0 40 1B 15 3 40 2B 17 0 a1 1A 13 2 41 24 12 0 4 1B 15 3 41 2B 17 1 a2 1A 13 2 42 24 12 1 a2 1B 15 5 4 2B 17 4 --~ 43 1A 13 4 43 24 12 2 43 1B 15 4 43 2B 17 6 a 1A 13 6 44 24 12 4 44 1B 15 5 44 2B 16 5 45 1A 13 4 45 45 24 12 1B 15 8 6 a5 2B 16 8 46 1A 13 6 46 24 12 8 46 1B 15 9 46 2B 16 9 --~ \EipinySanizsd=,Doss vot contain TSCA CB -- `1.2R5a4i3n6b:oEwarTlryouL,ifOencSourgheynTcohxiucsimtyykiss --_ TABLE 22 Dupont11953 5.0 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST `Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 17 1 40 2A 16 J 40 1B 18 0 40 2B 16 1 41 1A 17 2 41 2A 16 1 41 1B 18 0 41 2B 16 2 Q 1A 17 4 42 2A 16 1 2 1B 18 2 --~ 4a2 21BA 1716 26 43 2A 16 4 43 1B 18 5 43 2B 16 5 44 1A 17 7 4" 2A 16 4 44 1B 18 7 44 2B 16 8 45 1A 17 7 45 2A 16 4 45 1B 18 7 45 2B 16 10 4466 214A 1176 88 46 1B 18 8 46 2B 16 10 _ ESSpEDY anlized.Does otcontain TSCA CBI TT iHo2R5a4i3n6b:owEaTrrloyutL,iOfne-cSotrgleynTcohxiucsimtyykiss DuPont-11953 TABLE 23- DILUTION WATER CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Da Replicate Transfer Removed Larvae Remaining 46 A 15 0 0 15 46 B 1s 0 0 1s 47 A 15 a7 B 1s 0 0 0 15 0 15 48 A 15 0 0 15M 43 B 1s 0 0 15 49 A 15 4 B 1s 0 0 0 15M 0 15 50 50 A 15 B 1s 0 0 0 15" 0 15 ~~ 51 A 15 51 B 1s 0 0 0 15 0 1s 52 A 15 52 B 15 0 0 0 15 0 15 53 A is 0 0 15 53 B is 0 0 15 54 A 1s 0 0 1s 54 B 1s 0 0 15 55 A 1s 0 0 1s 55 B 1s 0 0 1s 56 A 15 0 0 1s 56 B 1s 0 0 1s 57 A is 0 0 15 57 B 1s 0 0 15 58 A 1s 0 0 1s 58 B 1s 0 0 1s 59 A 15 0 0 1s 59 B 15 60 A 1s 0 0 0 0 1s 15 60 B 1s 0 0 15 61 A 15 0 0 15 61 62 B 15 A is 0 0 0 1s 0 is rm 62 B 15 0 [1 1s -_-- EampanySanitized. Doss not contain TSCA CAI t2o5R4a3i6n:bowEaTrrloyutL,iOfen-cSotralgeynTcohxiucsitmyykiss -- `TABLE 23 (Continued) DuPont-11953 DILUTION WATER CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Tarvae Dead Cumulative Tive at Larvae Dead Larvae Da Replicate Transfer Removed Larvae Remaining 6 A 15 0 0 15 6 B 15 0 0 15 64 A 1s 0 0 1s 64 B 1s 0 0 1s 6 A 15 0 0 15 65 B 15 0 0 15 66 A 15 0 0 15 66 B 1s 0 0 15 67 A 15 0 0 1s 67 B 15 0 0 15 68 A 1s 0 0 1s -- 68 B 15 0 0 1s 69 A 1s 0 0 15 6 B is 0 0 15 70 A 15 0 0 15 70 B 15 0 0 15 7 A 1s 0 0 1s 7 B 1s 0 0 1s 72 A 15 0 0 15 7 B 15 0 0 15 7 A 15 0 0 15 7 B 15 0 0 15 7 A 15 0 0 1s 7 B 1s 0 0 15 75 A 15 0 0 15 75 B 15 0 0 15 76 A 1s 0 0 15 76 B 15 0 0 15 7 A 1s 0 0 1s ud B 15 0 0 1s 78 A 15 0 0 15 78 B 15 0 0 is --_ Company Sanitized.DoesnotcontTSaCAiCnBI -_-- SoB0RaRasaitininb:owETTxrroyuL,iOtOnecSnaogrcerTcohoruxsimyypktissss --~ TABLE 23 (Continued) owDeuwrolnio11s953s DILUTION WATER CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus. `mykiss, EARLY LIFE-STAGE TOXICITY TEST Day Replicate Larvae at Transfer Dead Larvae Removed Cumulative Dead Larvae Live Larvae Remaining 79 A is 0 0 15 9 B 15 0 0 15 80 A 15 0 0 15 80 B 15 0 81 A 15 0 81 B 15 0 82 A 15 0 0 15 0 15 0 15 0 15 82 B 15 83 A 15 83 B 15 84 A 15 0 0 15 0 0 15 0 [J 15 0 0 15 ~~ 84 B 15 0 0 15 85 A 15 0 0 15 85 B 15 0 0 is 86 A 15 0 86 B 15 0 87 A 15 0 87 B 15 0 88 A 15 0 88 B 15 0 89 A 15 0 0 15 0 15 0 15 0 15 0 15 0 15 0 15 89 B 15 0 0 15 9% A 15 0 [J 15 90 B 15 0 0 15 + Scolo chdes: curved sine, conser al, and bet spin) --~ - `TEmpinySanitzoe. DsHotcontain TSCA cat "`2o0R5Ra4ai3in6nb:boonwETaTrrrolouyuttL,iOOfnnecc-SootrrahlgyyennTccohhruuisscmmiyypkliissss ~~ DuDubpoonwtl-i11o9s53s TABLE 24 RIAPIANCBOONWTRTORLO:UTL,AORnVcoArLhyMnOchRuTsAmLykIisTsY, AEFATRELRYTLHIIFNE-NSITNAGGFEOTROXTIHCEIT9Y0-TDEASYT Larvae at Dead Cumulative Live Larvae Dead Larvae Day Replicate Transfer ___ Removed Larvae Remaining 46 A 15 0 0 15! 46 B 15 0 0 15!" 47 A 15 47 B 15 0 0 0 1s 0 15!" 48 43 A 15 B 1s 0 0 0 15 0 is 49 49 A 1s B 1s 0 0 0 15 0 1s 50 50 A 1s B 1s 0 0 0 15 0 1s ~~ 51 51 A 15 B 1s 0 0 0 15TM 0 15 52 52 A 15 B is 0 0 0 15" 0 15 53 A 15 53 B 1s 0 0 0 15! 0 1s 54 54 A 15 B 1s 0 0 0 1s 0 1s 55 A 15 55 B 1s 0 0 0 15 0 1s 56 56 A 15 B is 0 0 0 15" 0 1s 57 57 A 15 B 1s 0 0 0 15M 0 15 58 58 A 1s B 1s 0 0 0 15" 0 1s 59 59 A 15 B 1s 0 0 0 1s 0 is 60 60 A 15 B 15 0 0 0 1s 0 1s 61 61 A B 15 1s 0 0 0 0 15 1s 62 ~~ 62 A 15 B 15 0 0 0 15M 0 15 `CompanySaniizes. Donitscontsain Tac oar ~~ o1HR02aR5ia4ni3n6b:boonEwaTTrrrlooyuLu,iOfOennSccaoorgrhebynpTconhxhiucsuismtyymkpissss -- DDwuopowniti19o5s3y TABLE 24 (Continued) RIAPIANCBOONWTRTORLO:UTL,AORnVcoArLhyMnOchRuTsAmLykIiTssY, EAFATRELRYTLHIIFNE-NSITNAGGFEOTROXTIHCEIT9Y0-TDEASYT Tarvas at Dead Larvae Cumulative Dead Live Larvae Day Replicate Transfer Removed Larvae Remaining 663 AB 1s 15 0 0 0 0 * 15 64 64 A 1s B 15 0 0 0 0 1s 1s 65 65 A 15 B 15 0 0 0 0 15 1s 66 66 A 1s B is 0 0 0 0 1s 1s 67 67 A 15 B 1s 0 0 0 0 15 1s 68 - 668 A 1s BA 1155 0 00 0 00 1s 11ss I] 7 B A is 15 0 0 0 0 is 15 70 7 B 1s A is 0 0 0 0 1s 15 7 7 B A 15 15 0 0 0 0 is 1s 2 7 B A 1s is 0 0 0 0 15 15 7 7 B A 15 1s 0 0 0 0 15 is 74 75 B is A 15 0 0 0 0 is 1s 75 76 B A 1s 15 0 0 0 0 15 1s 76 7 B A 15 is 0 0 0 0 15 is 7 7% B A 15 1s 0 0 0 0 15 1s 78 B is 0 0 is BompanySentizes. Doesnot contain Tc op -- 1i4R250a36:o TtryotLGt SeeTroxi _ "TABLE 24 (Continued) Dupes IPA CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead `Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining kl A 15 0 0 15 79 B 15 0 0 15 80 A 15 0 0 15 80 B 15 0 [J 15 81 A 15 0 0 15 81 B 15 0 0 15 82 A 15 0 0 15 82 B 3 0 0 15 83 A 15 0 0 15 83 B 15 0 0 15 84 A 15 0 0 15 --_ 84 B 15 0 0 15 85 A 15 0 0 15 85 B 15 0 0 ] 86 A 15. 0 0 15 86 B 15 0 0 15 87 A 13 0 0 15 87 B 15 0 0 15 88 A 15 0 0 15 88 B 15 0 0 15 89 A 15 0 0 15 89 B 15 0 0 15 90 A 15 0 0 15 90 B 15 0 0 15 + ESrcomleloonttsoelndoenso:rainvsdsoine crcal,an btpinta) --_ BaganySanitzed. Boos notconai TSACal - `H0.2R5i43b6:aEaTlryouLt,ifOen-cSoargispnTcoxhiucsitmyykiss ~ TABLE 25 Dupont1953 0.30 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead `Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 46 A 15 [J 0 15 46 B 15 0 0 15 47 A 15 [J 0 15 a7 B 15 0 0 15 48 A 15 0 0 15 48 B 15 0 0 15 49 A 15 0 0 15 49 B 15 0 0 15 50 A 15 [J 0 15 50 B 15 0 0 15 51 A 15 0 0 15 ~~ 51 B 15 0 0 15 52 A 15 0 0 15 5532 AB 1155 00 [J0 1155 53 B 15 0 0 15 54 A 15 0 0 15 54 B 15 0 0 15 55 A 15 0 0 15 55 B 15 0 [J 15 56 A 15 0 0 15 56 B 15 0 0 15 57 A 15 0 0 15 57 B 15 0 0 15 58 A 15 0 0 15 58 B 15 0 [J 15 59 A 15 0 0 15 59 B 15 0 0 15 60 A 15 0 0 15 60 B 15 0 0 15 61 A 15 0 0 15 61 B 15 0 0 15 62 ~~ 62 A 15 B 15 0 0 0 [] 1s 15 Too "EERSaniized, DTi t Coa ntainTSCACBI 25436: BryLieSige Toi to Rainbow Trout, Oncorhynchus mykiss P TABLE 25 (Continued) DuPont-11953 0.30 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead `Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 63 A 15 0 63 B 15 0 64 A 15 0 64 B 15 0 65 A 15 0 65 B 15 0 66 A 15 0 66 B 15 0 67 A 15 0 67 B is 0 68 A 15 0 ~~ 68 B 15 0 69 A 15 0 69 B 15 [1] 70 A 15 0 70 B 15 0 n A 15 0 7 B 15 0 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 72 A 15 0 0 15 7 B 15 0 0 15 73 A 15 0 0 15 73 B 15 0 0 15 74 A 15 0 0 15 74 B 15 0 0 15 75 A 15 0 0 15 75 B 15 0 0 15 1 76 AB 1155 00 00 515 77 A 15 0 [J 15 7" B 15 0 0 15 78 A 15 0 0 15 78 B is i* 1 14* --_ EmorySantee. Doss notcontain rsa oa erre are r--d -- tHo.2R5a4i3n6b:owETlryouL,ifOencSourgheynTcohsiucshtmyykiss ~~ TABLE 25 (Continued) Duont11953 0.30 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining kil A 15 79 B 15 0 [J 15 0 1 14 80 A 15 0 [1 15 80 B 15 0 1 14 81 A 15 0 0 15 81 B 15 0 1 14 82 A 15 0 0 15 82 B 15 0 1 14 83 A 15 0 0 15 83 B 15 0 1 14 ~~ 84 A 15 84 B 15 0 [J 15 0 1 14 85 A 15 0 0 15 85 B 15 0 1 14 886 BA 1155 00 01 1145 87 A 15 0 0 15 87 B 15 0 1 14 88 A 15 0 [J 15 88 B 15 0 1 14 89 A 15 0 0 15 89 B 15 0 1 14 90 A 15 0 0 15 90 B 15 0 1 14 + One fish isin (escaped) but counted as dead -- [BampanySenltzed,Donotecons tain TSCA CBI - {1o.2R5a4i3n6b:oEwaTrrloyuLt,ifOenSctoargleynTcoxhiucsitmyykiss --_ TABLE 26 DuPont11953 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FORTHE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Tarvae Dead Cumulative Live at Larvae Dead Larvae Da Replicate Transfer Removed Larvae Remaining 46 A 15 0 0 15 4 B 15 0 0 15 47 A 15 a7 B 15 0 0 0 15! 0 1s a8 A 15 0 0 15" 43 B 1s 0 0 15 49 A 15 0 0 1st PE B 15 0 0 1s 50 A 15 0 0 15" 50 B 1s 0 0 1s st A 15 0 0 15" ~~ 51 B 15 0 0 15 52 A 135 0 0 15" 52 B 15 0 0 1s 53 A 15 0 0 15M 53 B 1s 0 0 1s 54 A 15 0 0 15" 4 B 15 0 0 15 55 A 15 0 0 15" 55 B 15 0 0 1s 56 A 15 0 0 15 56 B 1s 0 0 15 57 A 15 0 0 15 57 B 15 0 0 1s 58 A 15 0 0 15" 58 B is 0 0 15 59 A 15 59 B 1s 0 0 0 15" 0 15 60 A 15 0 0 15 60 B 15 0 0 15 61 6 A 15 B 1s 0 0 0 15" 0 15 ~ 62 A 15 6 B is 0 0 0 15" 0 15 - es "BompanySanitized. Doesnot contain TSCA Cr: tHo2R5a4i3n6b:owEaTrroluytL,ifOen-cSotragheynTcohriucsitmyykiss Dupont 11953 --_ `TABLE 26 (Continued) 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Tarvae Dead Cumulative Tive at Larvae Dead Larvae Da Replicate Transfer Removed Larvae Remaining 6 A 15 0 0 15" 63 B 15 0 0 15 64 A 15 0 0 15 64 B 15 0 0 1s 65 A 15 0 0 15 65 B 1s 0 0 is 66 A 15 0 0 15 66 B 15 0 0 15 67 A 15 0 0 1507 67 B 15 0 0 1s 68 A 15 0 0 15M --_ 68 B 15 0 0 1s 6 A 15 0 0 1 3 B 15 0 0 15 700 BA 1155, 00 7 A 15 0 7 B 1s 0 72 A 15 0 7 B 15 0 7 A 15 0 7 B 15 0 74 A 15 0 7 B 15 0 75 A 15 1 75 B 15 0 6 A 1s 0 76 B 15 0 7 A 15 0 7 B 15 0 8 A 15 0 78 B 1s 0 00 1155 + 0 1518 0 15 0 151 0 1s 0 1587 0 15 0 15h 0 1s 1 14 0 15 1 14 0 1s 1 14 0 1 1154 0 15 --~ `EompanySantized.Doss not contain sca car Tr Hto-2R5a4i3n6b:oEwaTrrloyutL,ifOen-cSotargheyTnocrhiucsitmyykiss DuPont-11953 ~~ TABLE 26 (Continued) 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Da Replicate Transfer Removed Larvae Remaining 7 A 15 0 1 14 7 B 15 0 0 15 30 A 15 0 1 14 80 B 15 0 0 15 81 A 15 0 1 14 81 B 15 0 0 1s 82 A 15 0 1 14 82 B 15 0 0 1s 83 A 1s 0 1 14 8 B 15 0 0 1s 84 A 15 0 1 14 -- 84 B 15 0 0 15 85 A 15 0 1 14 85 B 1s 0 0 15 86 A 15 0 1 14 86 B 15 0 0 15 87 A 15 0 1 14 87 B 15 0 0 1s 88 A 15 0 1 14 88 B 15 0 0 1s 89 A 15 1 2 13 89 B 15 0 0 15 90 A 15 0 2 3 % B 15 0 0 is a7 LSycionligosoinsb(oitnctloumdeosf: ecsutrvcehdasmpbienr, corkscrew al, and ben spne/tail) ~~ -- "EgmpanySanitized.Dossnot contain Ta=* AR: as SHtoo2RR5aa4ii3nn6bb:oowwBTaTrrrooyuLuti,,fOOennccSooarrgthepynnTccohxhiuucssimmtpyykkiissss ________ -- TABLE 27 DDuuoPomniti11o9s53s RAI1N.3BmOgW/LT:ROLUATR,VOAnLcoMrOhyRnTcAhuLsImTykYisAsF, TEEARRTLHYILNINFIEN-GSTFAOGRETTHOEXI9C0I-TDYAYTEST Larvae Dead Cumulative Tive Day Replicate at Transfer Larvae Removed. Dead Larvae Larvae Remaining 46 46 A 15 B 1s 0 0 0 0 15 15 47 47 A 1s B 15 0 0 0 0 15 15 48 43 A is B is 0 0 0 0 is 15 49 49 A 1s B 1s 0 0 0 0 1s 1s 50 50 A 1s B 15 0 0 0 0 15 is ~. 51 51 A 15 B 1s 0 0 0 15 0 15 52 52 A 15 B 1s 0 0 0 15% 0 1s 53 53 A 15 B is 0 0 0 15" 0 1s 54 A 15 54 B 1s 0 0 0 151 0 15 55 55 A 15 B 15 0 0 0 15" 0 15 56 56 A 15 B 1s 0 0 0 15% 0 1s 57 57 A 15 B 1s 0 0 0 15% 0 1s 58 58 A 15 B is 0 0 0 15 0 1s 59 59 A B 15 1s 0 0 0 15% 0 15 60 60 A 15 B 1s 0 0 0 15 0 15 61 61 A 15 B 1s 0 0 0 15% 0 15 ~ 6622 AB 15 is 0 0 0 151 0 1s EE Eotnpany Saniizet. Dosshot contain Tsca cm Lt2oo5RR4aa3iiw6nb:boowwETaTrrrlooyuuL,,iOfOnenccSootrrahhgyeynnTecohhxuiucssimmtpyybkiissss --~ `TABLE 27 (Continued) DDuuPonot-1m195o3 RAI1N.3BmOgW/LT:ROLUATR,VOAnLcoMrOhyRnTcAhuLsImTyYkisAsF, TEEARRLTHYILNINFIEN-GSTFAOGRE TTHOEXI9C0I-TDYAYTEST Tarvae Dead Cumulative Tive Day Replicate at Transfer Larvae Removed Dead Larvae Larvae Remaining 63 6 A 15 B 1s 0 0 0 15" 0 1s 64 A is 64 B 1s 0 0 0 1st 0 is 65 A 15 65 B 1s 0 0 0 iste 0 1s 66 A 15 66 B 1s 0 0 0 Iselin 0 is 67 67 A 1s B 15 0 0 0 15'4iake 0 1s --_ 68 68 A 15 B 1s 0 0 0 1511 0 1s 69 69 A 15 B 1s 0 0 0 15a 0 is 70 0 A B 15 15 0 0 0 1514 0 1s 71 7 A 1s B is 0 0 0 15% 0 is 72 7 A 15 B 1s 0 0 0 ad 0 1s 73 A 15 7 B 1s 0 0 0 15i4= 0 is 74 2 A 15 B 1s 0 0 0 1514 0 1s 7 75 A 15 B 1s 0 0 0 15M 0 15 76 A 15 6 B 1s 0 0 0 15% 0 1s 7 7 A 15 B 15 0 0 0 15" 0 1s 78 A 15 78 B is 0 0 0 15% 0 1s Po. ESMinySates.Dossnot conten rene ans -- Te,------ LHo2RR5aa4ii3nn6bb;oowEwaTTrrrolouytuL,iOfOenn-ccSotorarglheyynnTcoexhhiuucssitmmyypkhissss --~ `TABLE 27 (Continued) DDuuppoownlti1o19s53y RAI1N.3BmOgW/LT:ROLUATR,VOAnLcoMrOhyRnTcAhuLsImTyYkisAsF,TEEARRTLHYILNINFIEN-GSTFAOGRETTHOEXI9C0I-TDYATYEST Larvae at Dead Larvae Cumulative Dead Tive Larvae Day Replicate Transfer Removed Larvae Remaining 7 A 15 7 B 15 0 0 80 80 A 15 B 15 0 0 81 81 A B 15 1s 0 0 82 82 A 15 B is 0 0 83 8 A 15 B 1s 0 0 ~ 84 84 A 15 B 1s 0 0 85 85 A B 1s 1s 0 0 86 36 A 15 B 1s 0 0 87 87 A 15 B 1s 0 0 88 38 A 15 B is 0 0 89 A 15 89 B 1s 0 0 90 % A 15 B 1s 0 0 24 DSemaflolrminedsiizlel((wihgehntcsoimdep)ared to appropriate contro(s) H Swimminga wate surface 0 15" 0 1s 0 15% 0 is 0 15" 0 1s 0 15 0 1s 0 15' 0 1s 0 15 0 1s 0 1s 0 1s 0 15% 0 15 0 15" 0 is 0 15% 0 1s 0 15' 0 1s 0 15 0 15 --~ LampenySentzes. Dossmotconta Tscace - Tw H`025R4a3in6b:owEaTrrloyuLti,fOenSctoargheynTcoshiucsitmyykiss Dupont-11953 --_~ TABLE 28. 2.5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead `Cumulative Live at Larvae Dead Larvae D: Replicate Transfer Removed Larvae Remaining 46 4 47 a 48 4 49 4 50 50 sl ~ 551 52 53 53 54 54 55 55 56 56 57 57 58 58 59 59 60 60 61 61 ~ 6622 A 15 0 B 15 0 A 15 0 B 15 0 A 15 0 B 15 0 A 15 0 B 1s 0 A 15 0 B 15 0 A 15 0 AB 1iss 00 B is 0 A 15. 0 B 1s 0 A 15 0 B 1s 0 A 15 0 B 15 0 A 15 0 B 1s 0 A 15 0 B 1s 0 A 15 0 B 15 0 A 15 0 B 1s 0 A 15 1 B 1s 0 A 15 0 B 15 0 A 15 0 B is 0 0 15" 0 1s 0 15M 0 15 0 15" 0 15 0 15" 0 15 0 1s" 0 1s 0 15" 00 1515 s 0 15 0 15'elenm 0 15 0 sian 0 15 0 JE 0 15 0 15!vient 0 15 0 phiae 0 1s 0 Ed 0 1s 0 Ista 0 15 1 14100 0 1s 1 141 0 1s 1 il 0 is - eEa tpanySapced. Doss motcontai 75a car Hto2R5a4i36n:bowEaTrrloyutL,iOfnec-oSargheynTcohxuicsitmyykiss -- TABLE 28 (Continued) DuPont-11953 RAI2N.5BmOgW/LT:ROLUATR,VOAnLcoMrOhyRnTcAhuLsImTyYkisAsF, TEEARRTLHYILNINFIEN-GSTFAOGRE TTHOEXI9C0I-TDYAYTEST Larvae Dead Cumulative Live at Larvae Dead Larvae Da Replicate Transfer Removed Larvae Remaining 63 A 15 6 B is 0 0 1 1408 0 1s 64 A 15 64 B 15 0 0 1 14h 0 15 65 A 15 0 1 147 65 B 15 0 0 15 66 A 15 [1 66 B 15 0 1 14180 0 1s 67 A 15 67 B 1s 0 0 1 141 0 15 --_ 68 A 15 68 B 1s 0 0 1 140 0 15 6 A 15 0 1 14s 69 B 15 0 0 15 70 A 15 0 1 ad i B 1s 0 0 1s 7 A 15 7 B is 0 0 1 140 0 15 7 7 A 15 B 1s 0 0 1 141 0 15 7 7 A B 15 15 0 0 1 1418 0 1s 74 A 15 7 B 1s 0 0 1 1417 0 15 7s A 15 75 B 1s 0 0 1 141 0 15 76 A 15 1 2 13 76 B 15 0 0 15 77 A 1s 0 2 13 7 B 1s 0 0 1s 8 A 15 0 2 13 8 B 15 0 0 15 -- [EgmpanySanitized. Doesnolcontain TSCA om Tos 110.2R5a4i3n6b:oEwaTrrloyuLt,iOenSciorgheynTcoxhiucsitmyykiss -- TABLE 28 (Continued) Dupont11953 2.5mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 7" A 15 0 2 13 7 B 15 0 0 15 80 A 15 0 2 13 80 B 15 0 0 15 81 A 15 0 2 13 81 B 15 [J 0 15 82 A 15 0 2 13 82 B 15 0 0 15 83 A 15 0 2 13 83 B 15 0 0 15 84 A 15 0 2 13 ~~ 84 B 15 0 0 15 85 A 15 0 2 13 8856 BA 1155 00 02 1135 86 B 15 0 0 15 87 A 15 0 2 13 87 B 15 0 0 15 88 A 15 0 2 13 88 B 15 0 [J 15 89 A 15 [J 2 13 89 B 15 0 0 15 90 A 15 0 2 13 20 B 15 0 0 15 w& RScaopliidosriesspiinrcaltuidoens: curved spine, corkscrew il, and bent spinel) Lyingon otooftest chamber ~ `EompanySanitizes. Does vot contain TSCA CBI BIE StHoo2RR5aa4li3n6bb:oowEwTaTorrlooyuuL,,iOfOnenSccoaorrgbhepynnTccohhruiuscsimmyypkbiissss_____ -- TABLE 29 DDuuoPmonetl1i19s5s3s RAI5N.B0mOgW/LT:ROLUATR,VOAnLcoMrOhyRnTcAhuLsImTyYkisAsF, TEEARRTLHYILNINFIEN-GSTFAOGRETTHOEXI9C0I-TDYATYEST Larvae Dead Cumulative Live Da Replicate at Transfer Larvae Removed Dead Larvae Larvae Remaining 4 A 15 46 B 1s 0 0 0 0 1s is 47 4 SA B 15 1s 0 0 0 157 0 1s a8 43 A 15 B 1s 0 0 0 15M 0 15 49 49 A 15 B 1s 0 0 0 15" 0 1s 50 50 A 15 B 15 0 0 0 157 0 15 51 51 A 15 B is 0 0 0 15" 0 is - 52 52 A 15 B 1s 0 0 0 15th 0 1s 53 53 A 15 B is 0 0 0 15a 0 is 54 54 A B 15 1s 0 0 0 pias 0 1s 55 55 A 15 B 15 0 0 0 15th 0 1s 56 A 15 56 B 1s 0 0 0 15a 0 1s 57 57 A 15 B 15 0 0 0 Isha 0 15 58 58 A 15 B 1s 0 0 0 isthe 0 1s 59 59 A 1s B 1s 1 0 1 0 14 15 60 A is 60 B 1s 0 0 1 0 14 1s 61 61 A 1s B 1s 0 0 1 0 14 is 62 ~ 62 A 15 B is 0 0 1 0 14 is HE CHRSenhize. Doss Hol contain TSCA OF 1102R5a4i3n6b:owETxrloyuLt,ifOenSctoargbeynTcohxiucsimtyykiss Dupone 11953 PS `TABLE 29 (Continued) RAI5N0BmOgWLT:ROLUATR,VOAnLcoMrOhyRnTcAhuLsImTyYkisAsF,TEEARRTLHYILNINFIEN-GSTFAOGRETTHOEXI0C-IDTAYYTEST Laravtae LaDrevaade Cumulative Dead Live Larvae Da Replicate Transfer Removed Larvae Remaining 6 AB 15 1s 0 0 1 0 14 15 66 AB 15 15 0 0 1 0 14 1s 6655 AB 11s5 00 01 11s4 6 66 A 1s B 1s 0 0 01 11s 67 AB 15 1s 0 0 1 0 14 is 668 AB 15 1s 0 0 1 0 14 15 ~~ 6 A B 1s 1s 00 01 11s4 77 AB 15 15 0 0 1 0 1 is 7 7 A 15 B 1s 0 0 1 0 14 15 77 AB 1155 00 01 1154 7I AB 1155 00 01 11s 77 AB 1s 15 0 0 1 0 14 15 77ss AB 1s 15 0 0 1 0 14 is 77%% BA i1ss 00 01 1154 77 AB 1s 15 0 0 1 0 14 15 778 AB 1155 00 01 i1s4 ~ EBaySanizoa.oe otcos ntain Ts car ee eee Hto2R5a4i3n6b:owEaTrrloyuLt,ifOenScuorgheynTcohxiucsimtyykiss DuPont-11953 -- TABLE 29 (Continued) 5.0 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Da Replicate Transfer Removed Larvae Remaining 7 A 15 0 1 14 7 B 15 0 0 15 80 A 15 0 1 14 30 B 15 0 0 15 81 A 15 0 1 14 81 B 15 0 0 15 82 A 15 0 1 14 82 B 15 0 0 15 83 A 15 0 1 14 83 B is 0 0 1s 84 A 15 0 1 14 --_ 84 B 15 0 0 15 85 A 15 0 1 14 85 B 15 0 0 1s 86 A 15. 0 1 14 86 B 15 0 0 15 87 A 15 0 1 14 87 B 15 0 0 15 88 A 1s 0 1 14 88 B 15 0 0 15 89 A 15 0 1 14 89 B 15 0 0 15 90 A 1s 0 1 14 9% B is 0 0 is abha YRaoplikdseascppirreasteinotn(not absorbed) d7d LLyoisnsgofoenqbuoiltitbormioufmtest chamber -- EampanySantized,Doos not contain sca my has H25436: BaryLif SageTosicy StooRRaaiivnbbooww TTrroouwt,,OOnnccoorrhtyyncnhcuhsumyskmissss_ ~ TABLE 30 uDuePwonlt-i1g19s53s DILUTION WATER CONTROL: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW "TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number p LSetnangdtarhd (cm) Wet Weight gram: 1 A 5.0 1.6281 2 A 47 1.2671 3 A 42 0.9640 4 A 4.6 1.2515 56 aA 4433 11.30564520 7 A 48 1.3434 8 A 48 1.4075 9 A 438 1.3933 10 A 4.9 1.6317 ~ 1121 Aa 4459 1123281672 13 A 38 0.6517 1154 aA a475 11..41530629 1 B 42 0.9278 2 B 44 11217 3 B 4.1 0.9130 4 B 43 1.0570 55 BB 44.15 01.28248270 7 B 4.1 0.9251 98 BB 4459 11.05669193 10 B 4.7 1.4861 1121 BB 4479 11.37804416 13 B 4.5 1.1155 14 B 43 1.0424 13 B 48 1.6013 --~ -- Eompany Sanhizsi. Doss not contain TSCA CBr ws tHo2R5a4i3n6b:owEaTrrloyuL,ifOSntcaorgiepnTchousimykiss Dupont 11953 -- TABLE 31 AT TEST CONCLUISPIAOCNONFTORROTLH:EF0I-SHDALYENRGATIHNSBAONWDTRWOEUITG,HTOnScorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST LSetnangdtahrd WeWiegtht Number | Rep (em) gra 21 AA 440 01.70941077 3 4 A A a7 44 13784 12848 5 6 AA a3s9 01.17664664 78 AA a423 11.00129035 105 A A 41 48 038548 15978 ~~ 1 A 48 12 A 42 1.4999 05730 1134 aA 46 50 14529 1.6970 1s 1 a B is 44 14693 12643 23 BB aa72 1L.a07a0s2 4 s B B 41 40 08778 08106 6 7 B B 37 39 0.5888 0.7406 85 BB 4a3 01.90576538 110 BB 45 46 L1270 11423 12 13 B B 45 43 L1291 12498 11s4 BB 4474 0193982335 --_ Ee EmpeySaniized. Doo notcontain Ta ray 0H2R5a4i36n:bowEaTrrloyutL,iOfen-cSotrahgyenTcohxiucsimtyykiss PS TABLE 32 DuPon.11953 0.30 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Standard Wet Rep Lecnmg)th `gWreaight 1 A 45 12727 2 A 4.6 12737 3 A 39 0.7769 4 A 4.1 0.9947 5 A 5.0 1.7682 6 A 47 7 A 49 1.3858 1.5973 8 A 38 0.6702 9 A 38 0.7024 ~ 10 A 11 A 5.0 42 1.6720 11219 12 A 47 1.5625 13 A 49 1.7257 14 A 52 1.7653 15 A 40 0.7628 1 B 49 1.8952 2 B 41 1.0090 3 B 4.7 1.3474 4 B 42 5 B 4.1 1.0847 0.9477 6 B 4.8 1.6443 7 B 4.1 0.8719 8 B 39 0.7682 9 B 38 0.6331 10 B 43 1.0042 11 B 50 12 B 5.0 1.6000 1.7710 13 B 48 1.4707 14 B 4.0 15 B 2 0.8385 A a Fish diedpriorto test end -- -- Company Sanitized. Does not contain TSCA cal Te -- -- L1Ho02R5Ra4ai3in6nb:boowwETaTrrrlooyuu,L,iOOfnen-ccSootrrahkgeyynnTcochxhiuucssimmtyypkbiissss___ --~ TABLE 33 DuDuboontw1n1s95s3y 0.63 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Replicate SLteanngdtahrd (cm) WeWiegtht gram: ~ . ~~ 1 A 41 2 A 40 3 A 48 4 A 4.8 5 A 45 6 A 4.6 7 A 4.7 89 AA a510 10 A 4.0 1112 AA 4369 13 A 42 14 A - 15 A a 1 B 4.5 2 B 4.9 3 B 37 4 B 38 5 B 50 6 B 34 7 B 4.8 98 BB 4473 10 B 44 112 BB 4479 13 B 45 14 B 4.0 15 B 39 a Fishdied prior to test end 1.0369 0.9292 1.5758 1.7722 1.1733 1.5429 1.5225 01..87868235 0.8060 01..85620269 1.0751 - 1.2613 1.5593 0.6215 0.7014 1.7190 0.6475 1.4464 11.31901185 1.0695 11..63172819 1.3758 0.7730 0.8766 BompanySanitized.Doesnot contain TSCA CRY ~ ow HSo2R5a4i3n6b:owETartoyuL,iOsnScoargheynTcohxiucsimtyykiss DuPont 11953 ~ TABLE 34. AT TEST CONCLUSI1O.N3 mFgO/LR:TFHIESH90L-EDNAGYTRHASIANNBDOWWETIRGOHUTT,S Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Standard Wet Number p Lenegmth Wreainght 1 A 2 A 950 11.17846616 34 A A 39 4s 06941 1.2027 5 A 5 A a3s5 015.2221970 7 A 8 A 4a8s 1135507222 105 AA 43s6 0152458385 ~ 1 A 36 06809 12 A 51 1.7007 1134 AA i45s 11.03232696 1s1 A B 50 39 16127 09927 2 B 3 B 6 38 13223 07958 3 B 5 B 3439 0192923228 67 BB aiss 01.42787580 58 B B 47 4s 12440 13043 110 BB 3376 0066149727 1123 BB a4s5 11.32803035 11s4 BB 49 as 16133 1.5263 ~ - EMpanySanltized.Doesnotcontasicn cay tHo2R5a4i3n6b;owETarroluytL,ifOen-cSotrahgeynTcohxiucsimtyykiss DuPont11953 TABLE 35 2.5 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Standard Wet Length Weight Replicate (cm) gr 1 A 49 1.6575 2 A 41 0.8647 3 a 47 1.5467 4 A 35 04912 5 A 4s 13753 6 A 39 0.7499 7 A 46 12555 8 A 43 09677 9 A 50 17813 --_ 10 A 47 12550 1 A 44 11513 12 A 43 09255 13 A 39 0.8248 14 A + 1s A + + 1 B 49 15774 2 B 42 1.0898 3 B 39 07435 4 B 36 05565 5 B 39 08335 6 B 39 06569 7 B al 07471 8 B 46 1.2627 9 B sl 1.9099 10 B 35 05225 1 B 37 06235 12 B 46 13092 13 B 50 09864 14 B 3s 05731 is B 50 1.6540 --_ a Fish diedpriorto test end "EaMmpanySanitizsd. Doss not contain TSCA C81 -ww t2o5Ra4i3n6b:owETarroluytL,iOfen-cSotrahgeynTcohruiscimyykiss DuPont-11953 --~ TABLE 36 5.0 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 0-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Standard Wet Length Weight Number | Replicate (em) grams) 1 A a1 13881 2 A 41 0.8751 3 A 47 1.3667 4 A 46 1.1537 5 A 43 1.0605 6 A 45 11634 7 A 45 1.2005 8 A 46 12882 9 A 46 13625 10 A 47 13692 --~ 11 A 44 13012 12 A 4s 1.4060 13 A 40 0.8065 14 A 46 13488 1s A # 1 B 42 0.9436 2 B 45 12183 3 B 41 11129 4 B 35 0.5063 5 B a1 0.9804 6 B 37 0.7074 7 B 40 0.8737 3 B 35 0.5591 9 B 47 13672 10 B 43 1.1058 1 B 49 1.4207 : 12 B 42 1.0398 3 B 50 1.8293 14 B 37 0.5741 is B 45 12472 --_ a Fishdied prtiooterst end CompanySanitized. Doesnotcontain TSCA CRY Tae ~ 4 ~ 3 23 33 it 5i: E24H i z 5 2i5t g EEL 2iE2 ERAT SAsuR uuuEfEEE8E "gg Hil 2 g 8883888558 2 |.a gz EB ged 323234433 & 2 5 SF ARRARRSNRT 9 :fZ H RRRRwgangs 2 2 ZzgF f22R2R2R2A2i,e,E2Y2 |i] { 3 5 2Eez 1 czl E9H8H9)u.. : 9: mszHozfis 3 geist i BE5z5eEz5sEiRiEfEsYr|| 72 EmDinySanitzsd,Dossnot contain ver amy 1H02R5a4i3n6:boEwaTrrloyutL,ifOen-cSotargheynTcor xhiucsitmyyke iss --~ DDuuPPonotm.1i1i95s3 -- FIGURES ~ "CHRpaRY Saihized,3Does HoT-- Contain TSCA CRI S13 1fo.2R5a4i3n6b:oEwarTlryoLuiOtnecSoargpencTohxiiscitmyiss --_ FIGURE 1. Dupone11953 PHOTOGRAPH OF AN EMBRYO AT APPROXIMATELY 22-HOURS POST-FERTILIZATION BT a Ce aEmaE aa te am e. saiaSn ed K l Ee o i; Saale edie benineyne n|y Sipe psn a be BeSanria E adases Cede TL ~~ rNE ER RR ~ Lompeny Saniized. Dossnotcantain sca car Ee `102R54a3i6n:bowEaTrrloyuLt,iOenSctoargehynTcohxiucsitmyykiss -- FIGUR2E Duponc 11953 REPRESENTATIVE ANALYTIC;Al ALIBRATION ST: JARD CURVE FOR H-25436: B! Ci] aoolmoipbforiauiinoendnctnuoDravfoem:e-1.3A53O24N8+I0R0E6=+ 12(+Lo7g.s80865 +006 * x+ 34757.8 euvsepotynpees: t2ypned:OrEdxert,OeriSrgtidnn,:AaErxelcalude, Weighting: 14, Axis rans: Nona A ss65310- Response -- o065 5 Th 55% Tho 50 _ goaoimtipfracotiuieonnntdcoufnrDavoem:too-3rBm2i4n4a8toxn12 +TO6.4O2I4N78,465008 * x + 18073.2 B Ruerspeoynpsea:t2y0ed:OrExdteerr,naOlrSitgai.n:AErxecalude, Weighting: 15 Aus trans: Nons. 717650 Response]. 137) T 5% Ti 5% TE 5% ppm Company Saniizs. Doesnotcontain Tsca ay -- = -- -- H{028R4a3i6n:bowEaTrdaysL,iOencSoargheyTncohsuischmyykiss PN FIGURE 2 (Continued) Duron 11953 REPRESENTATIVE ANALYTICAL CALIBRATION STANDARD CUR FOV R H-25E 436: A Bf Cy oaoimcbpiroaftuconnidcounnartmeooe:t"fo283P1NPx4T20SR+"IR~c3hy.o1c403066*x4+ 86 6385.81 c ReUsrpeonpes:e p2on:d OErxdteorm,aOrliSgiin,: AErxecslude, Weighing: 11 Ad tans: Nona sors ety Response ~~ e386050 5 Te on TE ER) som ~ . . ------------Eom-- Toaunsys-- anitze-- g Possn-- otcont-- ain TOs-- p oAgy C--. 52506 Ba LiteSageTnisty to Rainbow Trout, Oncorhynchus mykiss DuPont-11953 FIGURE 3 REPRESENTATIVE HPLC CHROMATOGRAM OF A CALIBRATION STANDARD SOLUTION wo es ssradis . _ [BO i sosmraee of prinaly ST lass, Callston saat wissen R;R [ ris: e YET eisTio 2% . o- ETEA TT min 0 _ rae Bute at a re emtention reat time of approximately 3.58 minutes. Calibration standard solution contains } CotfipanySanitized. Does not contain TSCA AR -_-- ;seeelmm r eo s;g 1H02R5a43i6n:bowEaTrroluytL,ifOen-cSoargtepTnocxhiucsitmyykiss Dupont11953 ~ FIGURE 3 (Continued) ToTonnTE 100 REPRECSAELNITBARTAITVIEONHPSLTCANCDHARRODMASOTLOUGTRIAOMN OF A Ii 39753008447 Tiaras FESRofS ch1a4n9n7e0l40ET08i87c % o T8o t3Zr he e abo eae ke r ser r eas r min ~ TI 25a 4conc3entra6tion of 0.103 mg. cemesememe en ~~ . . Company Sanitized. Does notcontain TscA cat me -- {o2R5a4i5n6b:owETryow,LiOfnecSoarge Toximciss Dupon-1953 PP. FIGUR4 E TioTosiT REPRESENTATIVE HPLC CHROMATOGRAM OF A DILUTION WATER CONTROL SOLUTION FISIRof heananenn naES5 .98305004 0 The Zhe Sho ihe 5h 55s in ~ he ee enin ty1a 7 mies. Toros To 251A a1 enamel ES+ Tie 3.4080400 % ) o The he 34s ih Sho sh _[OI cn tim ie --~ . --_-- . Company Sanitized. Does not contain TSCA CBI Hto.2R5a4i3n6b:oEwarTrloyuLt,iOenScuorgheynTcohxuicsitmyykiss Dupont11953 -- FIGURE 4 (Continued) TTo0703 11 REPRDEISLEUNTTIAOTNIWVAETHEPRLCCOCNHTRROOMLASTOOLGURTAIOMNOF A FISIR 05 channel5s Tic 220704004 % o Tho 760 3he ahs 555 5.50 min PN [-- luteat etenionimeofspproimtely 5.08 mites. -- -- . EE . Company sanitized. Does not contain Tsca car 1H02R5a4i5n6b:owETarrolyuL,ifOnecSoarghyenTchouiscmtyykiss Dupont 1953 -- FIGURE 5 REPRESIEPNATWATAITVEERHCPOLNCTCRHORLOSMOALTUOTIGORNAM OF A T1i00703b15 FAISIR of channels.EST Tic 3.45004004 % o Th" e 2ho be ake 50 eho min --[--.....luteataretentiontimeofsproximately 1.97 mimes. Ti0703b15 10 F2iSIR of 5 channels.EST+ic 3.3700+004 % [om --~ -_-- 76 2he ibe abo 560 5%0 min eluteataretention timeofspproximately 3.58 minutes. . : EompanySanitized. Donote contsain TSCA cB B1E5R25h036s: atTyoonLOGtSauge Tcohy is Pe FIGURE 5 (Continued) opworeess REPRESENTATIVE HPLC CHROMATOGRAM OF A IPA WATER CONTROL SOLUTION 110703615 10 : F3:SIR of5 channels ES+ TIC 2.641e+004 # v ain --_ [OP .c..c conso psscy soi ~~ CompanySanllized. Does not contain TSCA ~~ - mem geee se s I1oR5R2aa5m63o6nTTtroywLiOrorSatagpeeTicoerrepyltiss Pp FIGUR6E oDuwpee15s5 REPRESENTATIVE HPLC CHROMATOGRAM OF A H-25436 TEST SOLUTION doe: w2H0i1.3H937050 6.113+006 . Te TT PS me Besccn05mo ohin nsofpiricnt197its. Tsslospl coins 25436 sosiisiheso . Te TE I pe -- ~ : CompanySanitized. Do note contsais TSC res HEeE ee e esser s 2to5R4a3i6nb:owBaTrroluytL,iOfen-cSotrakgeynTcohxiucsimtyykiss DuPont-11553 ~~ FIGURE 6 (Continued) REPRESENTATIVE HPLC CHROMATOGRAM OF A H-25436 TEST SOLUTION 170703635 FIR 015 channelT5isc7 418773816083.513 % o 173 Too 250 sho - who - sho & sho min Tominal conceationeluteoatfa0.r3etmegnt/iLo.n timeofapproximately 5.08 minutes. Test solution sample contains H-25436 at a --_ . CompanySani?tized. Doesnot contain Tera Tas -- H-25436: Early Life-Stage Toxicity 0RReanintboonwTTrroouut,,OOnnccoorrlhyynncchhuussmmypkbissss _________ ~~ DuDPuPoomntl-11199s533 --~ APPENDIX CompanySanitized. Dossnotcontain Tena "135- -- tHo:2R5a4i3n6b:owBaTrrloyuLti,fOenScuorgheynTcorhiicsitmyiss ~ Dupont 11953 --_ APPENDIX A Certificateof Analyses -- -_-- TB Senki Los not contain TA Te CompanySanitized. Dossnot contain *=s art LH0o2RR5ea4ii3nn6b:onwTEToarowu,LiOcfnso-eSortavngieepTorxeiscbiutmysiysiss ~ py DuPont Specialty Chemicals - Ba7r18 ot Coy puDowunio119s53s r S SmEy. omenShaan. Contit, CERTIFICAOTFE ANALYSTS sie vote Js oCutoer= e --O)SS~a D --~ DuPootOnder 4 BXPM=__ Tot Weight, Ls. ____= wegen. ref eat p13a1n01e338 WR r--e m-- o Appearance & Fae Bs [i-- ssa Ora TTC ComrpanySanitized. Does Totcontain TSCA cpt Company Sanitized. Do nots cants are =a + ns.