Document Lge9D44mDZL0ExBvm7jxkXorX

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-206A. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was cc 785-22. The testsample isFC-206A. Currentinformatioinndicatesitisa mixtureof 0.92% PFOS, 18.75% diethyleneglycolbutylether,75.44% water,1.83% Hydroxy foamer,1.75% sodium octylsulfate,1.2% poloxyethylene monooctylphenylether,0.06% sodium laurylsulfatea,nd 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Acute static GLP: No Year completed: 1979 Species: Pimephalespromelas Supplier:CommercialsupplieirnBrady,NE Analyticalmonitoring: Temperature,pH, and dissolvedoxygen. Exposure period:96-hours Statisticamlethods: LC50valuescalculateudsingprobitcomputerprogram. Test fishage: Juveniles Length and weight: Average length 41 mm Loading: 0.64g/L Average weight 0.64g (wet) Pretreatment:None. Test conditions: Dilutionwater: Carbon filterweedllwater Dilutionwater chemistry: Hardness: Alkalinity: Conductivity: 236 mg/L as CaC03 221 mg/L as CaC03 430 pmhos/cm Lighting:Not given. Stock and testsolutionpreparation:The testmateriawlas added directly tothedilutiownater on a weight/volume basis. Concentrations dosing rate:Once Stabilityof the testchemical solutions: Not noted. Exposurevessels:Glasstanks(36x2lx2c4m) containi1n6gLoftest solution. Number of replicates:2 Number of fishper replicate:16 Number of concentrations: fiveplusa blank control Water chemistry during the study: pH range (0-96 hours): 7.9 - 8.0 (controelxposure) 7.6- 8.0(1350 mg/L exposure) Temperature range (0-96hours): 20 - 20 OC Dissolved oxygen range (0-96hours): 5 - 9.1 mg/L (controelxposure) 0.9- 9.1mg/L (1350 mg/L exposure) Remarks: Fishwere surfacinginallexposure concentrationse,xceptthe controlt,hroughoutthestudy.The dissolvedoxygen was below 60% saturatioinntheexposure concentrationsby 24 hours,and remained low for the durationofthe study. RESULTS Nominal concentrations: Blank control4,20, 560, 750, 1000, and 1350 mg/L. Element values: 96-hour LCm = 1303 (1094 - 1930) mg/L (Replicate1) 96-hourLC5o = 1228 (1098- 1485)mg/L (Replicat2e) Element valuesbased on nominalconcentration. Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hour LC50forfatheadminnow was determined tobe 1303 mg/L. witha 95% confidenceintervaolf1094 to1930 mg/L forone replicate, and 1228 mg/L witha 95% confidenceintervaolf 1098 to 1485 nig/Lforthe otherreplicate. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksrecordofmethodology used. There isa lackofcharacterizatioonfthetestsample and the studylacks analyticaclonfirmationofthe amount offiuorochemicalproportioninthe solution. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number 4970S, 1979. OTHER Lastchanged: 6/26/00 MVINNNWTAL LAwmiroRy WTIC IDUCIV MTA SWIM LR qq 70 Tnm Test Test Test Organisalh k\,eA N.A;,^-Aow"xm Date locid.-6-7-24 Batch No.-fL Avg. Wt. paw Avg. It* 4*A Of No. Orgmism per dos* Test SolutionVolmoi& Utoxs/All-Mus Tak Tmk Kamimet 3WI ca md 2'kcm deep Tim Startod_lllo ev,@ Pat@ Started DilutionWater Catbm-fil!&iMi.y*llM&AOT .7 17 (Tur Iz0 DataCovieted D.O. e4 Pli lavestigato @.(FUIISip& 11 tAN A W 60 (95%Ccefidneusslu)ilo3 3cy 'Init. Conc. a1/1 tal I iZ.144 Asomt Total Added No. 0*4--&d s 48 Hours 0.0. Total 0.00 T"M 000 tai Control 426 5;to6 ?ro 1,000 /-?S-o 6,7,20 I I-a6,400 1 It,G,oo 171 too ev.9 0, c0 C) 7..r.T1.s I ? r..T.6, t-2-6 0 2@ C) 0 tv z.r 2..2!2C 0 -7.t,*2.4 0 17tv ? .4 ?1.?-o. r 176 I.c- l.2 o - 1-0 ei '7 Test OC. im- 2- a C- =. T=,- -Z oloc c@TA 12 SizmatIuDraete- 2 '7 Co@nts: Effects other than Mortality init. conc. -2 /1 1 Symvtous or Reactions Control isignature Date Comments: -24-Hr. ECSO Total No, Affected 48 Hr. 72-Hr. 0 'A cr L,7 Cl. 0 7- 96-Hr viz. lie, Lk BNVINUMTAL LASOITATORY &"TIC TOXICITYDATA SHEET Type Test --Pktsri&l Tested cc, 7,f Test Organis Date R*cld. fi.7--7 Batch No.1 Avg. Wt. rg4 -1 2- gr@ Avit.sito__I.tL-CN No. OrSanism per dose Test SolutionVol@.!b Uters/All-Gl"s Tmk Tmk K=Rsims: 36x2l en and 24 co deep 'rimeStartod_lLg O_ek,. Date stanod '7.1 c -*74 Data completw 7- le4-? Dilution Water Carbon-ftlte well Iten-(Tow.. -_zo c D -o. 1.1 MR. PH 0 lavestigator'@NA. t.S CA IJ (PullSipature) LCso ($S% Cmfidmco Units) 1-22 CJA --T"- Init. Conc. -MALII Total Asmat I Added 34 Total 90. Dead 71 0.0. Total 0.0. Total woo tai -No. Dead di no No ad ? Control z co- -7-12 (7 -7,9 49. 0 c C))14177.(64,07*--.0t-2bo I. r C) 12 C-6c) f, ,ttA o 0 7.6 21 C) C, 2.5- 12 ? rb lza C) 7.C, f.17 /k coo -71 4 r0 ZiD. Test Temp. OCO SisiatureA Data Coments: L,ffectsother than Nortality conc. ...!gI/ Control Symptoms or Reactions to 24-Hr.- ecso Total No. Affected 48 Hr. 72-Hr. -0 4@ I " D,Q- I 96-Hr. go C-2 Signature Date Comwnts: IM ENVIROMMEt4TFIL-LAPOPATOPY **+PF@OBIT A"RL-y-@IS -POP -DICIASSF4Y -DATR*O* NO [IF CURYES 7 4 NO. -OF CDNj',. FOrl R@LIPYE 1 -7 4 INPUT -DRTA-CONC. @HO. EXPO,ED, NO. rE-Arl -560,169-0 750@16,t .? -1350--16,tt COMMENTS FOP-CURYE-1 FC@-2r,.A F:I-H ..............t@.M .........F.RACTION - - CONG ..........C1.3MG.@ ........P.ESP014DI"r -FITTED -FPFIC.TIO" -560 ..........2..-748t99 ..... 0.1 ... ......0..0011-90394-750-1 ........2...e?500.;t......O.-062'i .......0..02755678-1-000 .........3. ............0. t2.3 ........0..,att%92 -1,350 .........3..-t3O3'34 ......0.-6075 .......O..-643ZI-2i CHI--90 -PPOI)ART-LITY -FS -> -0.-05 -M -OF ........I.NTERCEPT ....SLOPE . ......CHI- -SO -FOP ....r@Hl--i@o-FOP FTE"T FUNS (R> ...........(.0).........L.INEARITY .....PROBABILITY ...........-.27.-65447 ---- 9.-9516t9 ... -k.-6091-,Q - @ ....O.-447jO2 HgTFODWhIETY -FW-FOP -(N)@ -- -I..............6..-- -0.-2756303 - -------- 95 -PC-TW-LOWER -CDMF.--LFMIT ......... .......1.-098.-326 -PC-T.--UPPEP -COW.- -L-1-MtT ................1.-.4r4l;.-Ol-3 "13w -OF -COhC-.--FOR -CURVE -2 - -? -5 IhPtJT -EXPOSE rhO.-DERD Awr k4wA@k!: i@- -? -7"3Ort6r2 -? -Iooorl.6r2 '71 -1-35orf6vto CD"ME-"TS 4:cp -CIAWE .2 -? -FC--2060 -FTSH ..............LO.G .......... T II)h......FI.TWD CONC@ ........AC-O"wC.-.....@.@ -RESPO"DM - - - F TIOM' -420 ..........2..-@32491 ......0 ...... ......O@005238'640 - -560 ..........2..-748t98 -0.-0625 .......o...o29te56 -7'50 ..........-2w975O6l ...... 0 @ I-L5 ........-O.-tO59364 -1-00.0......;..3 ....... .....O.-k25 ........0..-2749694 -1-350 ...I ......3.-l-30334 ......0.-625 ..... 0.-5322085 C-NI-SO -PP.OBAPILtT-Y -1-S-)- MO-OF ........I.-MTEPC-EPT .....9.LOPE .... ...CHt -SO -FOR ....CHI -SO -FOP ITEPATION-3 .....(A)........ -(-B)...... ..LT"E-FIPITY ....44KMBI.LI-TY .5 ...........-.t6.-21-4.)4.3 .....5..-207192 .....3..-tS@"52 ......0.-36241?6 HETEP(16EhIE-TY -FACTOR -(H> t ..............6.-- -0.-2767631 - L-C-5 0 ----------1-302.-605 - q5 -PCT.- -LOWER -COW.- -LIPI*T-95 -PC-T@ -UPPEP. @C-OW.- -LfpttT @ ............@.t@094.-2M .............1..191- NO.- -OF -COMO.- -FOF@ -CLTP.YE -3 - 3 ItfPUT -DATP@CONC.-@ND.- -EXPOSED,Mo.-DEAD .7 -1000@2O.-O -1-350@i?O,-t COMMENTS -FOP -CUPYE -3 -? DAPO!T tA FC -206A ..............LO.S .........F.RACTION CONC ..........CO.NG ..........RE.SPOMDTMG .....F.TTTEI) -- -FPFIC-TI-E) -toon -- ....1-4 .....I.......0 ............O..-Oot? t350 .........3..-1303r34 ......0.-05 .... L .....0.-0"9 Ieoo .......... 3.-2552?3 ......0.-3 ..........0..-30341 CHI-SO -PPOBFIBILI-TY -1-S-> -0.-05 MG OF ........f.t4TEPC-C-PT ..... SLOPE ........C.HI- -SO -F IPEPATlDr4S - (A> ...........l.(B)........L.I-MEARI-T ...........-.31-.-30479 .....9..-459595 ......0..04;104 HFTFPDi3Et4lETY Ff4CTOP -(H). ...... ..... ..G.. .0., LC-50 cO5 -POT.- -LOWEP. -CONF.- -LIMI-T .................t?.W.-e q2 PCT.- -UPPER -CEINF. -LIMIT .................53.64.-SO 0'-G.- -OF @CONC.- -FOP -CURVE -4 - -2 .3 I-MPUT DATA-COtIC-.,rNO.- -E)iP()SEDrMD.-DE-AD -1-00OV200@O .135 0,2 0,-I-190 @209-5 C-@MMENTS -FOP CUPYE -4 'DAPHNI-A FC -206fi ............... L-06 .........FPR TIM .....F.IMB C'DNC...... ....C-ONO ..........RE.SP13"DtMG ....FRAC-TEON -1-000 .........3. ............0.............. -k35O .........3..-t3O334 O.-0024t ......0.-05 .........0..-043062 Ieoo .......... 3.-e55273 ......0.-25 .........0..-254648 r@iol.-So-PPOPAPtL.I.Ty ts E*4k@,ra y ...........-.28--lOl'95 ......B.-#'3457 .......0.-0740?7 NETEROGENIETY-FACTO P fH> .1 ............... LC-50 .. ......2.t54.-405 9J -PCT.- -LDIIE-R'CONF.- -L-IMTT ........... 1-824.-61-8 95 -PCT. -LIPKP -COMF.- LLMET .................le.S64.-95 L rA ffTFi4r 2 NTS - FOR- -C-.LIRVE-@ --HR LC-5(1---FC-206FI BLUEC, I LL--STi=I'IT C Mfl IY I S, LD&- - FPFiC.TiONRE@-SPDr4DINC, ------ C-1@.--25527e- (1.65 @-,-PREIBFiB-IL-1TY--l-'-k- -(I.C[5 FITTED -FPFIC'TION n -1712226-- @NTERCEP-T @.SLOPE - ------ -CRI ';-ZC!FOP (B) -L-INERRIT)@ --@5.76147 H E 1*1 E-T Y -FF4C:TOR I .6 (14Cf3 0.96Ci2308 C- Cl.1759374 i-327128- EW---'@CONFi.LIMIT-= IIPPER--@C:ONF-.iL.IM IT LIPV E -2 -NO. --E@PB'-'ED, NO IIEFID 1 (197 271 1314.239 FC--@-2068 -DRPHN IR,-STRT-1-C--@,-- c --FR FiC.-T 3 0334 Ll ri 0 127 5----'@ cu 15 4 F13 2 -4 N (I-FC,.--2(16Fi FFITHEFID '.-STFiITC - LOC-R cl:.Mr-@;;@.@@-1.@-.,'-C-O.N.T..--@.1-@.-- FRFICT ION RE*:-@:PDNDINCt @-2.E-E-3249 2. 74:? 188 3. 131-1:-334 ILI TY Cl. C15 fl (I.65E,2"-i Fl T-T-ED.FRRF-Z"TION -0-i@C1t155297-@-@@-Ct727705--Ct,.-25729ca (1.574(1.?.39-- I-TEPRT- I ONS I NI-EPCF-PT (.Fi) HETEPOSENIET*T' FRC:IDP (.H:@F 12E.2. R&r-T-@ L 0 tkE*lR C:DNF. LIMIT -f-IPPEP CONF. LIMIT ---'LOPEE, 4'- 3 2 -Ef CHI-- '-SC@FOP -LINERRITY CHI, SFA -FEIRp R 0 B R P.I u-I - .5i--144122 Cl.16154c;:,-,-- C, -0. 1346,.,::Ci2 11 3E--.1-75 14@43*. 742 R.ER14,Y ELFIPSED T I @IE"--0: 07: 51 (103!1-RC-C-.OLINT,-ERR@ TTY 4 2!, 0 -18 IN CI:29:51 PP-QUE!QR 1,10,47%-Pt Y$ 13 FOO SAt4PLE 7@ESe-1kiPTIGO 'IV %)@ . j@, -@o q U01.% 0 AZ IOASSAY RooM. I lo- ?a -@LL j4f-78 is -2LZ ---.45-jL-Vnioe -xsiita 0o q4r 2.24 0 ol d7r -%7- --m-Al- 0 177.7 !feawi-l. if &q0ir ATTACII?IENI -kl A" age a I -Ceti ILL 'Cost f 1 7-