Document LQr1w7DXzXkXZqjZOjZ78V9g

FOR DU POMT USE ONLY Copies to AR226-2898 E. I. du Font de Memours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkcon Road, Newark, Delaware 19711 Study Initiated/Completed 6/4/81 - 6/6/81 .terial Submitted by Polymer Products Department Wllmington, Delaware 48-HOUR LC50** TO DAPHNIA MAGNA Procedure; The test material, as a 1 mg/mL stock solution in laboratory test water, was diluted with laboratory test water to yield the desired exposure concentrations* After mixing, 200 mL of each concentration was introduced into each of two separate 250 mL glass exposure vessels. Two identical jars, containing only laboratory test water, were designated as controls* Ten daphnids (Daphnia magna) less than 24 hours old were randomly assigned into each vessel* Food was not provided during the test* Test solutions were not aerated and temperature was maintained at 20"C* Phocoperiod was maintained at 16 hours light:8 hours dark. Mortality counts and observations were made at 24 and 48 hours after the exposure was initiated* Dissolved oxygen and pH were measured in the control, low, medium and high exposure solutions at the beginning and end of exposure* The total alkalinity, hardness (EDTA) and conductivity of the control were measured at the beginning of the exposure. The 48-hour LC50 and 95Z confidence limits were calculated by probit analysis (Finney, 1971).t Results: Percent mortalities for Daphnia nagna exposed Co H-14,008 are presented in Table I. The 48-hour LC50 was 320 ppm with "952 confidence limits of 281 ppm and 362 ppm. The slope, of the dose response curve was 5.1 with 952 confidence limits of 3,7 and 6.5. At concentrations of 560 ppm and greater the test solutions were too cloudy to complete an accurate mortality count at the 24-hour observation* No daphnids were observed swimming in the 1,000 ppm vessels, therefore, mortalities were noted at 24-hours by Company Sanitized. Does no^ontam TSCA C8t withdrawing each daphnid via a manually operated suction tube and observing the animals* The chemical and physical parameters measured during the test are reported in Table II Bibited _ _ . exposure. slight acute toxicity to conditions during a 48-hour The 48-hour LC50 was 320 ppm with 95Z confidence limits of 281 ppm and 362 ppm* The slope of the dose response curve was 5.1 with 95Z confidence limits of 3.7 and 6.5. ** LC50 - The concentration which is lethal to 50X of a test population during the specified time period. t Finney, D. J. Probit Analysis, 3rd ed., 1971, Cambridge University Press. Report by; ^efwi/t^, 3 / ^,^'^Zi Robert L. Trivits Technician Approved by Study Director: /W^) ^ / '^^g^C \/ A. Of'./}' y7 Frank X. Philli^b Research Biologist Approved by: RLT:FXP:CDL;jrg -Date Issued: June 18, 1981 Chief, Aquatic Report C^PM^""". ^."o.^"*""^^ TABLE I RESULTS OF A 48-HOUR ACUTE WITH DAPHNIA MAGNA EXPOSED TO H-14,001 Nominal Test Concentrations PP 1000 750 560 420 320 240 180 100 Control Observed Mortality (Z) 24 Hours 48 Hours A* A 100 100 ** ** ** ** 0 0 0 0 0 6 0 0 0 0 0 0 100 100 100 100 100 100 60 50 40 30 20 40 20 20 0 0 0 0 '*Replicate exposure chambers containing ten daphnids each. ** A few daphnlds were observed swiooing, but the test solution was too cloudy to complete an accurate mortality count. 3 - Company Sanitized. Does noi contain TSCAC51 TABLE II WITH RESULTS MEASURED DAPHNIA MAGMA OF PHYSICAL AND CHEMICAL PARAMETERS DURING A 48-HOUR ACUTE TOXICITY TEST EXPOSED TO H-lA.OOOT Nominal Test Concentrations 1000 ppn (High) Dissolved Oxygen (ppa) 0 Hr. 48 Hr. 5.6 6.6* H 0 Hr. 48 Hr. 7.9 8.1* Total Alkalinity (mg/L as CaCO.) 0 Hr. EDTA Hardness (mg/L aa CaCO,) 0 Hr. <- Conductivity (umhos) 0 Hr. 420 ppa (Medium) 100 ppm (Low) Control 6.8 7.8 8.1 6.5 7.4 7.7 8.0 7.9 7.9 8.3 8.3 8.1 133 117 265 * Final dissolved oxygen and pH were measured at 24 hours because 1002 mortality had occurred within the first 24 hours of exposure. 4 - Company Sanitized. Does not contain TSCA CBS