Document LQkM4gxvNrg5d53dvr64QNow
AR 226 -- 0795
ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH)
TEST SUBSTANCE
Identity: MreifxetrurreedctoontaasinPiFnOgSP,erFfCl-u9o5r,ooocrtaansesaulcfoomnpatoen,enwhtiocfhFmCa-2y0a6lAs.o b(e1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: is FC-206A.
The 3M production lot number was cc785-22. The test sample Current information indicates itis a mixture of 0.92% PFOS,
11.87.57%5%sdoideituhmyloecnteylgsluylcfoalteb,ut1yl.2et%hepro,lo7x5y.e4t4hy%lweanteemr,on1o.o8c3ty%lhpyhdernoyxlyefthoearm,er,
0.06% sodium lauryl sulfate, and 0.05% benzotriazole.
cThhaerafcotlelroiwziendg scounmcmeantrryataipopnlsioesftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicity of the fluorochemical componentofthe test sample.
METHOD:
Method: U.S. EPA 1975
Type: Acute static
GLP: No
YSepeacriceosm:plLeetpeodm:is1m9a7c8rochirus
Supplier: Analytical
Dale Fatting monitoring:
Fish Farm, Brady, NE. Temperature, pH, and
dissolved
oxygen.
Exposure Statistical
period: 96-hours methods: LCs values
and
95%
confidence
intervals
were
calculated using the U.S. EPA (Duluth) probit computer program.
TLeesntgtfihsahnadgew:eiJguhvte:niles Average length = 45 mm
Loading: 0.8 glL
Average weight = 0.92 g (wet)
PTreesttrceoantdmietnito:nsN:one
Dilution water: Carbon-fitered well water
DilutioHnawradtneerssc:hemistr2y3:5 mg/L as CaCOs
Alkalinity: Conductivity:
221 mg/L as CaCOs 418 ymhos/cm
Lighting: A daily photoperiod of 16 hours light and 8 hours darkwith a 30-~~ --
minute transition period was maintained throughout the testing period.
Stock andtestsolution preparation: The test material was added directly tCoonthceendtilruattioinownastdeorsoinnga wreatieg:htO/nvceo.lume basis.
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Stabilityofthe test Exposure vessels:
chemical solutions: Not Glass aquaria (35x30x20
noted. cm) containing
16
Loftest
solution. Number of replicates:
2
Number Number
of of
fish per replicate: 10 concentrations: five plus
a
blank
control
Water chemistry during the study:
pH
ran7g.e9(80-.906
hours): (control
exposure)
Temper7a.t5u7re.9ra(n1g3e50(0m-g9/6Lhoeuxrpso)s:ure1)7*-18 C
Dissol5ve.d89ox.y9gmegn/rLa(ncgonetr(o0l-9e6xphoosuurrse)):
* 13500m.g1/7L.(3inmtger/mLed(i1a3t5e0cmongc/eLnterxaptoisounr)ee)x*posure values used
because the highest three concentrations used resulted in total
mortality by 48 hours.
oRbesmearrvkesd:inVtehreyelxopwosdiusrseoclovnecdeonxtyragteinoncson(ceentxractfioeornptsht(e0.c1on--tr1o.l8) mbgy/4L)8 hours.
RESULTS
Nominal concentrations: Blank control, 1000, 1350, 1800, 2400, and 3200
mg/L (listed in text as g/L)
Element values:
96-hour
LCso =
1300 (1100 -- 1400) 1200 (1000 - 1300)
mg/L (Duplicate mg/L. (Duplicate
1) 2)
Element values based on nominal concentrations.
Remarks: Substance
Testing was conducted on amixtureas described Remarks field. The values reported apply to that
in the Test mixture and
not
the fluorochemical proportion alone.
CONCLUSIONS
The test sample 96-hour LCso range for bluegill sunfish was determined to be 1200 - 1300 mgiL.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
-
DATA QUALITY
HReolwieavbielri,tys:amKipilmeispcurhitryanwkaisngn2o.t pTreospteirnlgymceheartasctthereiczreidterainadfotrhequsaltiutdyytleasctiknsg.
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analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, 1978.
OTHER
prereseteeem------
Last changed: 6/26/00
eIsi3
Fomenriia
TECHNICAL REPORT SUMMARY
TO: TECHNICAL COMMUNICATIONS CENTER -- 201.20N
important ifreipsporinrtedton both siodfpaepesr, end two copietso TCC.)
oe 2 2
Environmental Laboratory (EE & PC)
"0535
Commercial Chemicals
9970012600
FC-206A cc 785-22: Results of Aquatic 96-r. LC; Test} 003
D. L. Bacon
A. N. Welter
09362
wcomrry Boom
T Gow
HEE
) on ein ed
waenwotneomss:rom 3m ooteaiswe EE & PC-Div. (ELnavbi)ronmentalT"oaxqiucaittiyc)
CoRRENT oRIEETIVE To determine the acute LCg, value for the bluegill sunfist o(fLeFpCom-i2s06mAaccrcoc7h8i5r-u2s2). expBSed to varying concentrations
RSEePOhRTrABtSoTaRAmCTp. (3R0B0G..750 word This ntact narmrion GradbYthe Techical Commarea Gon
Tnhmaecraoccuhtieruss)tateixcpo9s6e-dhrt.o vLC.a)ijinfgorcbolnuceegnitlrlatisounnsfisohf (Leponi FC-206A cc 785-22 ranged from 1.2 to 1.3 g/1.
InformatiSon eiown
001594
|
FC-2064
-2-
November 2, 1978
CONCLUSIONS
LICn, a vraelpuleiscatfeodr bsltuaetgiicll96-shuonufrishLCy,{Pepaoqmuiasticmaotresotc,hirtuhse) cwaelrceula1.t2ed oarndder1.3ogf/1magrneistpuedcetivaerley.consMiadteerreidalstopporsesseesnstingan aninLsC.i) ffiofficatnhtis hazard based on the NIOSH aquatic toxicity criteria.
INTRODUCTION
pTheerfotresmtedsuobnsttahniscemaFtCe-r3i0a6lA eccval7u85a-t2e2d iistsanaquaamtbiecr tlioqxuiicdi.ty Taensdts the data generated form the basis of this report.
METHODS
~
A. Aquatic Testing
Tthheattdesetscrpirboetdocobly uUtSi/lEiPzAed197f5o.r this study was modeled after
B. Acclimation Procedure
Tahepribvlauetgeilhlatscuhenrfyi.s*h (SLteopcokmisfimsahowroecrheirhueal)d wienrea ofbitbaeirngeldassfrom hAolddaiinlgy tpahnoktopfeilrlieodd wofith16chaorubrosn-fliiglhttereadndwe8llhowuarsterdaratk w14i-t1h5% a C. . 3a0c-cmliinmuatteiontraanndsittieosntinpgeripoerdiowda.s mTahientafiinshedwetrheroufgehdou"tTetthrea-Min"# dtaeislty,perfiooodd. beiFnigshwiwterheheladcc4l8imahtoeurds fporrio1r5 dtaoysandpritohrroutoghotuetstitnhge.
FAnC-2a0c6uAt.e sChaorrbto-nte-rfmilt(e9r6e-dhowuer)llswtaatteirc bofioaksnsoawyn wcaosmpposeirtfioornmedwason u30sedx a2s0 ctmh)e dciolnuteanitnin(gAt1t6a:c1hmeonft d1i)l.uentAllorgdlaislsuenatquaplruisa t(o35xicxant cteosmtperdiseadt aeaschtudcyhemcihcaamlberc.oncTeenntrabtliuogni.lls,Tesutniffoirsmh wienresizrea,ndwoemrley atolxlioccaanttedatdoditeiaocnh. tesBtluecghialmlberexwpietrhiimnent3s0 wmeirneutecsondfuocltleodwinatg 1of7aCl.l teMsotrtasloiltuyt,iontsempweerraeturreec,orddeids.solvGeedneroaxlygeonbselrevvealtioannsd pH raeplpartoipvreiatteo.behavioral changes were similarly recorded when TFhCe-s2e06Astu(dcices78w5e-r2e2)pecrofncoernmterdatiinonrse:plic1.a0t,e u1.s3i5n,g t1.h8e, f2o.l4l,owainndg u3.s2ign/g1.theLCU.BJEPvAalu(eDsulwuitht)h p9r5o%bictonfciodmepnucteerlpirmoigtrsamw.ereOrcgaalnciuslmasted uasndedfrineetohfisdsitsuedayse.were considered to be gemerally healthy
#*CDoamlemerFcaitatligFiFsihsh fFoaordm,0fBrkandoyw,n Nceobmrpaosskiat,ion.
00159
FC-206A
-3-
November 10, 1978
RESULTS
Thhaed balnueagvielrlagesuwnefiigshht (oLfepo.m9i2s+.3m6acrgorcahmisruasn)d uaseldenginthtohfis4s.t5u+d0y.6 cm. Trheecomlmoeanddiendg lfaocatdoirngfofracttohreseofs0t.u8diegs/1wa(s3).0.6Tegm/1perbaetluorwethaendmapxHimum rreasnpgeecstitvherloyu.ghout this study were 17-18 C. and 7.6-8.0 pH units
oDfisstohlivsedstuodxyy.genTevsatlueasquadreicareassoeldutisohnasrployrig4i8n-ahloluyr wpoesrte cilneiatriation
(normal tion of
DO levels), became straw this study had assumed a
cgorlaoyriesdh-w(hliotweDOc)o,lora.nd
Tahtisterlmaitntae-r
ocxoylgoerncchoanngteentw.as AalctchoomupgahnietdhebyDOalerveellastivdeeteirnmcirneeadseweirnethien tdhiessolved
rduaengetoofwha0t.1w-0o.u3ldmbg/el,asstuhmeerde wtaosbenoaagpepnaerreanlt hiynpcorxeiacsesiintuamtoirotna.lity
The absence of an increased mortality attributable to low dissolved
othxeygecnalccuonltaetnetd rLeCl;ativoanlsuehsiparies traekfelnectaisonpsresofumptteistvecehveimidceanlceitnhdautced
mortality, per se'
1In.0-t1h.i3s arenpdli0.c3a,tedransgteudy1,.1-t1he,596g-/1hourresLpC.e)tfivveallyu.es wTehreese1.v2a,luersange tanranCscl,atevaltuoeLC1.R)tvhiasluersanogfe 1a2re00coannsdid1e30r0edpptmo. beChaenmiicnaslisgnpiofsicsaenstsing hazard to the aquatic environment (2).
REFERENCES
(1) US/EPA - Methods for Acute Toxicity Test with Fish, Macro6i6n0v/e3r-t7e5b-r0a0t9e,s a19n7d5.Amphibians, Ecological Research Series
(2) CRhergiisstternyseonf, ToH.xicE.,EffaencdtsE. ofJ. ChFeamiirccahlildS,ubsEtdas.nceps., XV'76anEddiCti,ion, UIn.sSt.ituDetpearftomrenOtccoufpatHieaolntahl, SEadfuetcyatiaonnd HaenadltWhe,lfaWraes;hinNgattoino,nalDC, June, 1976.
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ENVIRONMENTAL ENGINEERING LABORNIORY |
AQUATIC TOXICITY DATA SHEET
Type Test Stale 94h LC
Material Tested AC.JCL 4
BIE
Lot_cC 785% 22
"est Organism Lloegr lls vendor Baal, plete. Date Rec'd g/azfsy Batch Nous g-1g
Avg. We..19%.359 grams Avg. Size 4.51.58 cn.
Test Solution Volume / Liters/Tank No. Organisms per dose__/o
Tine Started77t.yeyg bate Started_jo/o/rs Date Completed_j-o--ju (75
Dilution Water.Carbon filtered well (Temp. 2 C ., 0.0. yy ppm, ph_25 )
o(FvuelslsiSsisgncaotrurl, lama Lpltoi5 $5 (95% Confidence Limits) [3 Li
mel. Added ||No. Dead |pH |ppm No.Dead |p ppm No.vead | pH| ppm | |No.Dead|pH
aANP 4PO PS 4PY PO IP 1:0
of.
21|
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re | signature & vate {| Ault refuel wodi sojing Vnussntie
oman om
Comments:
prpuhitt
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4
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ca)llmionnsnfssodt
dadan
2cgirnuraaicmdit|aQuo4dpolauetiiiotni,dbett as n we
Effects other than Mortality
eo
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par eS
Si ature 4 Date
Comments:
lawassnops]l{ avigfisndna loa 7n ? iowwound
A
i. a
----B --E ----S ----TCOPYAVAIABIE
001598
.
T ENVIROANQMUEANTITCALTOXEINCGIITNYEERDIANTGA S"HLAEBEOTRATORY
vs
TypeTest_Stetic vib teen
Material TesteJCd_4-C Lot 7 cc85721
Test Ongonian Blatt, Vendor Broce Date Recta ypaslzs paseh No_2-45 1
Avg. We. 9158,359 grams Avg. Size 4.5 t.5
cm.
Test Solution Volume_ [{
Liters/Tank No. Organisms per dose_[
Tine Starritdee4d4 Date Started_iells]g Date Completed_siy
Dilution atef Carbon filtored well (Temp. I$ C, D.0. rg ppm, pi 25)
aetlststenarrorCellobon BIC 55 (95% Confidence Linits) 123 Lone ToNrome||toca "10.0. [roe Ty 0.0.frotal 20 [ro TEs5
#/1 |[saded ||No. Dead |pit |ppn" ||No.Dead |pn| ppm |No.vead |p| ppm ||No.Dead bn |p
enrolPlNe,hallae lToeaelear foLhilleio]l0allu:) Lo
ales "Sa lnel.2 Jaw 1b
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cea lore {0 halal & rele Vslel.2ll 3 lal.
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Signature & vate || AUulbhejofulag||AWGtE,(oh2]28 Nanw dis
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Comments: Stato fx
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Eetects other than Mortality
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