Document LMGro9OL6dOyzd5dOJ76M9NX
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfbnatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-3031. (1Octanesulfonicacid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 2. The testsample isFC3031. Currentinformationindicatesitisa mixtureof0.96% PFOS, 20% diethyleneglycolbutylether,62.11% water,10% ethyleneglycol,1.92% propane sultonefoamer,2.02% polyoxyethylenemonooctylphenylether, 2.88% sodium octylsulfate0,.06% sodium laurylsulfatea,nd 0.05% benzotriazole.
The following isan abbreviated summary of muldple fathead minnow studies on FC-3031, Lot L These studies apply to a mixture with incompletely characterized concentrations of impurities.Data may not accurately retlectthe toxicityof the ffuorochemical component of the testsample.
METHOD:
Method: Not given. Type: Acute static GLP: No Year completed: 1976 Species: Pimephalespromelas Supplier.Not given. Analyticalmonitoring: Not given. Exposure period: 96-hours Statisticamlethods: LC50valuesdeterminedby graphicinterpolation. Testfishage: Juveniles Length and weight: Average length 48 - 50 mm
Average weight 1.4- 1.5g (wet) Loading: Not given. Pretreatment:None. Test conditions:
Dilutionwater: Aeratedcarbonfilterewdellwater Dilutionwater chemistry: Not given. Lighting:Not given. Stock and testsolutionpreparation:Not given. Concentrations dosing rate:Once Stabilitoyf the testchemical solutions:Not noted. Exposure vessels: Not given Number of replicates:1 Number of fishper replicate:20 pertestsolutionforone study.Not given forremainingstudies.
Number ofconcentrationsf:iveplusa blankcontrol Water chemistryranges duringthe studies:
pH range (0-96houm): 7.2- 7.4(controelxposures)
Temperature range (0-96houm): 21 -22 OC (controelxposures)
Dissolvedoxygen range (0-96houm): 5.0- 9.2mg/L (controelxposures)
RESULTS
Nominal concentrations: Blank control,100, 250, 500, 750, and 1000 nig/L fortwo studies(7/6-1Of76,7/27-31/76).Blank control2,50, 500, 750, 1000, and 1250 mg/L forone study (7/20-24/76).Blank control,100, 180, 320, 560, and 1000 mg/L forone study (8/10-14f76).
Element values: 96-hour LCw range = 560 - 800 mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on a mixtureas described inthe Test Substance Remarks field.The values reportedapply to thatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsample 96-hour LC50 range forfathead minnow was determined to be 560 to 800 mg/L.
Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133
DATA QUALITY
Reliability:Klimischranking 3. Testing lacksrecord of methodology used. There isa lackof charactedzabon ofthe testsample. Test solutionslack analyticaclonfirmationof the amount offluorochemicalproportioninthe solution.
REFERENCES
These studieswere conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, 1976.
OTHER
Last changed: 6/26/00
740101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
Type Test
96 Hours -
Material Tested
Test Organism Fathead Minnow
Avg. Weight
Date Started
Date Completed
Time Started
o -z9b,I
Plotted LC,,
Dilution Water Carbon Filtered - Well Water
Size
7ZZL,7)Z
Cone.
24 hrs
%
D.0
Survival pH ppm
48 hrs
%
D.0
Survival pH ppm
72 hrs
%
D.0
Survival pH ppm
96 hrs
%
D.0
Su;vival. pl@,@,:-'ppm
Control 106 1'7S.'6i1 too -ZT q'@ Ie)c::5
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7,a S:@I ioo 7.-@- sol k oo 7,2-
@oo
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loo'17L, loo 7.t
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7o
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701 si-o1 4 ri 17,o4,-@- --20 7,o4.Q) 0 7v @,q
Average Temperature
cl
L
Coments:
LC Calculation so
T+
@4:F7-
up
100
10
20 .30 40 50
60 @70
80
90
100
Survival
740101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
Type Test
96 Hours -
Material TestedFc:-
Test Organism Fathead Minnow
Avg. Weight
Date Started
712ol76
Date Completed
Time Started
--2@
Plotted LCS-0
Dilution Water Carbon Filtered - Well Water
-o3z.
Avg. Size
712-4 1
A liAc,
Conc.
24 hrs
%
D.0
Survival pH ppm
48 hrs
%
D.0
Survival pH ppm
72 hrs
%
D.0
Survival pH ppm
96 hrs %
Survival
D.0 ph_@ppm
Control
loo -7.@"2 -7,1
-7. @@l
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too -7-o '5.0-loo '7.613.o k(3o 7,o qS
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7,oq,si
too 7,o !5.z 65 7,o 4,(@
4.1 0
7,0 5.0 0 @5.47.5
0
-701, Soo 0 6.q S
Average Temperature Comments:
LC Calculation 1000 . so - - V"
IT 0
u .-1 -4
7
0
10
20 @30
40
so
60
70
80
90
100
Survival
740101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
Type Test
96 Hours -
Test Organism Fathead Minnow
MaterialTested GD*bOdW"
Avg.
Avg. Weight
Size
Date Started
-7/2-7/26 - Date Completed
Time Started
- Plotted LCS
Dilution Water Carbon Filtered - Well Water
2-/6
Conc.
24 hrs
%
D.0
Survival pH ppm
48 hrs
%
D.0
Survival -pH ppm
72 hrs
%
D.0
Survival VH ppm
96 hrs
%
D.0
Survival pH ppm
Control
-6,7.[oo -Z-6s, loo 7-@ loo -7,
7,2-6.2-1 tC)b 7,2 C7 ivo 'Z2
loo 7,
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7-2- @-c.'-
loo 7.0 6-al too 7-o Cl loo 7-0 S.-D ioo Zc,/L4.RI
0 1 @OD 6.7,6,o 90 i7.o 9,-Olgo 7.0 4,g 6o 7.14
0 5.0 0
Average Temperature a I ci Coments:
LC so Calculation
400
-47-:F
cis
0
-4-
i-4
4I
T
-T t
IOD
0
10
20 .30 40
so
60
70
80
90
100
Survival
740101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
Type Test
96 Hours
Material Test4@-
Test Organism Fathead Minnow
Avg. Weight
Date Started
421 ILI Z6
Date Completed
Time Started
f
AOI",
PlottedLC
Dilution Water Carbon Filtered - Well Water
C .. o -2:1-k
ASvigze
Cone.
24 hrs
%
D.0
Survival pH ppm
48 hrs
%
D.0
Survival pH ppm
72 hrs
%
D.0
Survival pH ppm
96 hrs D.0
Survival pH ppm
Control
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Average Temperature
Comments
h@z 41
S482.)A49. tV
LC so Calculation
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0
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T
10 0
10
20 .30 40
so
60
70
80
90
100
Survival