Document LKvnR1ZaY85VOk2NdjvnO55Kd

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredto as PFOS, FC-95, or as a component of FC-3003. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notgiven.The testsample isFC-3003, identifiebdy the laboratoryas R1331. Currentinformation indicatesitisa mixtureof 1% PFOS, 15% diethyleneglycolbutylether, 79.39% water,2% Sultonefoamer, 1.5% sodium octylsulfate1,% sodium decylsulfate0,.06% sodium laurylsulfatea,nd 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: OECD Guideline,202 Type: Acute static GLP: No Year completed: 1997 Species: Daphnia magna Supplier:In-houseculturemsaintainedatAScl-DETD, Duluth,MN. Analyticalmonitoring: Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticamlethods: No calculationpserformed,based on studyresults. Test conditions: Dilutionwater: DechlorinatedCityof Duluth,MN tapwater post- carbon filteare,ratedfor24 hours priorto use. Dilutionwater chemistry: Hardness: 44 mg/L as CaC03 Alkalinity: 42 mg/L as CaC03 Conductivity: 145 pmhos/cm pH: 8.05 Lighting:Cool-whitefluorescentlights.A dailyphotoperiodof 16 hours light and 8 hours dark was maintainedthroughoutthetestingperiod. Stock and testsolutionpreparation:The testsolutionswere prepared by directadditionoftestsubstance,followedby mechanical stirrinagtambient labtemperature. Exposure vessels: 250 mL beakers with200 mL testsolution. Number of replicates:4 Number ofdaphnidsperreplicate5: Number ofconcentrationosn:e plusa blankcontrol Water chemistryduringthestudy: pH range(0-48hours): 7.89- 8.11(contreoxlposure) 7.88- 8.05(1000 mg/L exposure) Temperature range (0-48hours): 20.2- 20.7 'C Conductivityrange (0-48hours): 133 - 154 pmhos/cm (controelxposure) 150 - 160 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48hours): 8.7- 8.9 mg/L (controelxposure) 8.6- 9.1 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations:Blankcontroland 1000 mg/L. Elementvalues: 48-hourEC50=>1000mg/L Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 48-hour EC50 was determined to be >1000 mg/L. No mortalitoyr sublethaleffectswere observed. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.Thisstudy meets the criterifaorquality testing.However, the sample puritywas notproperlycharacterizedand the studylacksanalyticaclonfirmationofthe amount offluorochemicalproportion insolution. REFERENCES Thistestwas conducted by AScl Corporation,DuluthEnvironmentalTesting DivisionD,uluth,MN atthe requestofthe 3M Company, Lab Request number R1331,1997. OTHER Last changed: 6/26/00. ASci Corporation/ASel-Duluth Environmental Testing Division AScl Study ID# 5010-068 STUDY ACUTE TOMCITY OF R1331 TO DAPHNLI MAGNA DATASTANDARD OECD GUIDELINE 202 STUDY COMPLETED March 27, 1997 TESTING FACILITY AScl Corporation/AScI-Duluth EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,MN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATION NUMBERS ASCI Study ID# 5010-068 3M Company Lab Request# R1331 Sponsor: 3M Company Sponsor Study ID# R1331 1.0GENERAL DUORMATION A$ci Corpomtion/ASci-Duluth EnvironmentaTlestingDivision ASclStudy ID# 5010468 StudyTitle Data Standard Sponsor Sponsor'sRepresentative Sponsor'sLab Request# TestingFacility PrincipaIlnvestigator ProjectDirector TestingFacilitDyirector DefinitivTeest Dates Data Validationand ReportReview Retentionof Raw Data and FinalReport L.omtionof Raw Data and FinalReport AcuteToxicityof R1331 to Daphnia magna. OECD Guideline202. 3M EnvironmentalTechnology and Safety Services,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133. Susan A. Beach, 3M Environmental Technology and SafetyServices,Building23E-09,935 Bush Avenue, St.Paul,MN 55133. Tel No. (612)778-7452. R1331. AScl Corporation/ASel-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811; Tel.No. (218)722-4040. Tina Galloway. Joe Amato. Donald Mount. March 20-22,1997. Alan Mozol. R rt ign@atue: IV Two yearsfrom thedatestudyiscompleted. AScl Corporation/AScI-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811;Tel.No. (218)722-4040. Sponsor: 3M Company Sponsor Study ID# R1331 2 ASel Corporation/ASci-Duluth EnvironmentTaelstinDgivision ASelStudyID# 5010-068 2.0OBJECTIVE(S) To determintehe48-hmedianeffecctoncentrati(oEnC50)and 48-hno observableeffect concentrati(oNnOEC) ofthetestsubstancRe1,331,forDaphniamagna. Thisstudywas conducteadccordintgotheAScl studyplanretainebdy theSponsorand OECD.. Guideline202 (OECD 1993). TestSubstance Test Organisms DilutionWater Exposure Chambers 3.0 TEST METHODS Properties:Clearamber-wlored liquid,specifigcravity= 1.02 (water 1),completelysolubleinwater,and stable. Testconcentrations:0 (dilutiownater control)and 1000 mg/L. High concentrationtestsolutionwas preparedby mass additionfollowedby mechanical sfa-rinagtambient lab temperature. Source: Healthy,lessthan 24-h old Daphnia magna neonatesobtained from AScl stockculturesmaintainedin water of testdilutionwater quality. Use in test:Gravid adultsisolatedin dilutionwater 24 b beforethe test initiationU.sed 5 organisms/replicataend 4 replicates/tecsotncentration. Handled witha smooth bore pipetwith an insidediameterof 6 mm. Did not feed during the test. Source: DechlorinatedCityof Duluth,MN, tapwater. The most recent chemical analysisisin Raw Data Package. CharacteristicsA:t testtime, the water had a hardnessof 42 mg/L (as CaCO3) and a pH of 8.11.Aerated for24 h priorto usingin the test. 7ype: 250-ml borosilicatgelassbeakers. Use in test:200 mi of dilutionwater or testsolution/chamber.During the test,kept covered,exceptwhen experimentalobservationswere made. Sponsor: 3M Company SponsorStudy ID# R1331 3 Incubation Observations Endpoint Calculations ASel Corporation/ASci-Duluth Environmental TestingDivision ASel Study [D# 5010469 Duration: 48 h. Daily photoperiod: 16 h lightand 8 h dark, maintainedusing cool-white fluorescentlamps. Temperature: 21 *C. Biological:Daily- gentlyagitatedthe exposure chambers and then counted immobilized and dead testorganisms (immobilizedorganisms were thosethatdid not swim within 15 seconds afterexposure chambers were gentlyagitated).Did not remove any daphnidsfrom theexposurechambers at24 h. Water chemistry:(1) At testinitiatiotno-talhardness,alkalinity(,2)dailytemperature,specificonductivityp,H, and DO. Alldeterminationwsere made accordingto APHA methods (1995). Formal calculationnsot needed due to lackof effect. 4.0 RESULTS Test Substance Toxicity(Pertinentdataare in Table 1) 24-h EC50: > 1000 mg/L. 48-h EC50: > 1000 mg/L. 48-h NOEC.* 1000 mgll. Ranges of Water ChemistryParameters Totalhardness(asCaCO3 mgll): 42-44. Alkalinit(yasCaC03 mg/L): 40-56. Temperature(OC): 20.2-20.7. DO (mglL): 8.6-9.1. pH: 7.96-8.11. &eciec conducti!12(a@slcm@: 133-160. jj Sponsor: 3M Company Sponsor Study M# R1331 4 ASci Corpomtion/AScl-Duluth Environmental TestingDivision ASel Study [D# 5010-M 5.0TEST QA/QC QA/QC Criteria Data Testorganismdead,immobilizedor trappedin 0% dead,immobilizedor trapped. controlexposuremust notbe more than10% The lowestDO concentratioanttest terminatiomnust be atleast60% of theair saturatiovnalueat testtemperature. The lowestDO concentratiomneasured was 97% of theairsaturatiovnalueat21.0'C. Testdurationmust be 48 h. ne testduratiownas 48 h. Referencesubstance(sodiumchloridet)oxicity AScl'supdatedreferencteoxicittyestcontrol test. chartisintheRaw Data Package. 6.0REFERENCES AmericanPublicHealthAssociatio(nAPHA). 1995.StandardMethods fortheExaminatio of Water and Wastewater.APHA, Washington,D.C. OrganizatiofnorEconomic Cooperationand Development(OECD). 198111993.OECD GuidelinesforTestingof Chemicals.OECD PublicatioInnformationCenter,Washington,D.C. Sponsor: 3M Company Sponsor Study ID# R1331 5 TABLE 1. ACUTE TOXICITY OF R1331 TO DAPHNIA MAGNA ASci Corporation/AScl-Duluth Environmental TestingDivision ASel Study ID# 5010-M ............ . . ....... 0 1000 Cu,mulative:NUmber anc: 24 b: 0(0) 0(0) :.w ][,,Mmc@bili7W. 49-h 0(0) 0 LO) Percentagesare in parenthesis. EFFECT AND NO-EFFECT CONCENTRATIONS WRFH CORRESPONDING 95% CONFIDENCE INTERVALS 24-h ECSO 49-h EC50 49-h NOEC Data:(@ng > 1000 mg/L > 1000 mg/L 1000 mg/L 95%. C-onfidenceinterval Not calculable Not calculable I Not !RLhcable Sponsor: 3M Company Sponsor Study ID# R1331 6