Document LKqnjZL1M8pgqoqVGaV1J21m7
MICROBIOS MICROTOX TOXICITY TEST
TEST SUBSTANCE______________________ _____________________
Identity: Perfluorooctanesulfonate; may also be referred to as PFO S or FC-95 or as part of the mixed product FM-3820 (see Remarks). (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3)
Remarks: The test sample is FM-3820, a mixture of the test substance in diethylene glycol butyl ether and water (approximately 24-28% test substance in diethylene glycol butyl ether and water). Calculations were made to adjust test values for the upper limit concentration of the test substance (28%) in the test sample and no adjustment was made for the presence of the diethylene glycol butyl ether or water as noted below. Calculations assumed that all toxicity was due to the presence of the Perfluorooctanesulfonate substance.
METHOD____________________________________________________
Method: Microbics Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1991 Species: Photobacterium phosphoreum Analytical monitoring: Not available Statistical methods: Not available Test organism source: Microbics Microtox Reagent Test conditions: Per Microbics method
R E S U L T S _________________________________________________
Nominal concentrations: 125,250, 500,1000 mg/L
Test Sample Element values: 30-minute E C 10 = 161 mg/L 30-minute E C 50 = >1000 mg/L
Perfluorooctanesulfonate concentration adjusted Element value:
30-minute E C 10 =45 mg/L 30-minute EC50 = >280 mg/L
Remarks: Values reported are for the test sample and then adjusted assuming all toxicity is due to the perfluorooctanesulfonate component. No calculations were made to adjust for the concentration of the diethylene glycol butyl ether in the test sample.
001596
CONCLUSIONS The test sample containing 28% Perfluorooctanesulfonate (upper limit) exhibited a 30-minute EC 5ofor Photobacterium phosphoreum of >1000 mg/L. If you assume all toxicity of the mixture is due to the
Perfluorooctanesulfonate, the adjusted 30-minute E C 50 value is >280 mg/L
(1000 mg/L X 0.28). Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking 4. Values are from a summary only. Original test report and raw data was not found. Additionally, data is for a mixture and toxicity cannot be positively attributed to Perfluorooctancesulfonate as the diethylene glycol butyl ether could also contribute to the toxicity. R EFER EN CES________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number J1608, 7/24/91. O T H E R ____________________________________________ Last changed: 5/3/00
001597
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0 8 /0 8 /1 9 9 1
EtS/IBCHBOTlL LABORATORY FINAL REPORT
LAB REQUEST NO. J1608 RDPDEREAEEQOSTPUECJAEE:KRCSFTEMTCOCONR2EO0TIVN3::EA09DPA59E:N37:500DF04DO1/13OH00V0/C1C0S9NE9LE1LN/ISSAB See IR H2864 fo r D. magna te s t r e s u lts .
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SAMPLE DATE CCCE
OESOUPTION RESULT
MDL CR (C .I.)
1 BUTYL CARB. PH 8887
AMLGKARGOGKAKSSA- YEC- SOIC50
>>11000000 HMGG//LL
((CC ..II.. --))
2 D0PCN0L m RM 3021
*MALKGHA7ETA0KSSA- YEC- SOIC50
911.1MMGG/L/L
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AMLICGTAOETAOSKSA- YEC- SOIC50
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EM 3820
AMLIGCRAOEIOAKSSA- YEC- SOIC50
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6 NA.-OCTYLSOIF RM 55040
AFMLIISGCHRAOEATACCSOXSTA-EYTB-CESSOITC-5096 BOOR 41887000HMMGGG/L//LL
(((999555%%%CCCTTI((14(650800---91680300))))))
7
FC-203
--------------------
ALGAE ASSAY - IC50
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8 FC203 W/O TO VW 160390
FISH ACOTE TEST - 96 BOOR >1000 KG/L
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BEST COPY AVAILABLE
08/08 /1 99 1 ......... ENVUOM-MAL laboratory fin a l report
LAB BEQUEST NO. J1608 SAMT DAIS CODE
OCNTINCED EESCRIPTICH RESULT
8 FC203 M/O TO VM 160390
`AMLKG3AETCAKSSA- YB-C5K0 50
336200 MMGG//LL
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ENVIRONMENTAL LABORATORY MtCROBJCS MCROTQX9 SYSTEM
J 1608 7 /24/91
TOXICTTY TEST RESULTS
PERCENT UGH! LOSS
BUTYL CARBTTOL
T C ST C O N C .
RM 8887
U g O . k A ifi 18In a n .m in
1 .2 5
DUPCNOLM E
TOLYTRM 2CLE
RM ER
FU ER
rO C T Y L S U F A T E
RM 3021
RM 27513
FM 411S
FM 3820
RM 32067
m in 1 A .m in aft-m ln IsB iO JUbJBlB I S b a i l I j A | ^ g | 8 3& flU n A sS tS JJScB iO lU b fllO E a t s i i - m i o - n
20 41 54
2 .5 39 67 79
3 .1 2 5
32 30 32
5 .0 74 9 92
6 .2 5
51 49 52
1 0 67 92 95
1 2 .5
68 67 70
2 5 82 80 82
3 1 .2 5
U 11 22
50
6 2 .5
24 36 46
100
125 250
12 10 14 17 10 19
9 .7 1 7 2 2 4 .4 7 .1 5 J t 3 7 5 5 6 7 10 18 20 13 17 23 54 57 76
500
31 2 9 31
12 16 21 19 24 31
1000
-
23 27 30 23 30 42
EC 50
1 0 0 0 * 1 0 0 0 *1000 9 .0 U
1.1 M
M L I 1000 1008 1000 1000 1000 1000 2 00 120 80
9 5 % C . 1.
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FC 203 teT O L Y T W A Z O L E
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U 1ft 2 6 17 30 42 3 0 4ft 6 4 4ft 6 4 7 6 1008 800 320
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