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AR 226 - 0828 ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) IESTSUBSTANC 0E 0 Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-600 (2-15-77 RAW). Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 %-benzotriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. This study is the first of 3 daphnia studies conducted to determine the toxicity of FC-600 after being treated through a SCAS reactor. There is no synthetic sewage medium present. M0 ETHOD:0000000 Method: Not noted. Type: Static acute GLP: No Year completed: 1977 Species: Daphnia magna Supplier: Not noted. Analytical monitoring: Dissolved oxygen Exposure period: 48-hours Test organism age: Not noted. Statistical method: ECs calculated by graphing data and by probit analysis. Test conditions: Dilution water: Carbon-filtered well water, aerated. Dilution water chemistry: Dissolved oxygen: 9.4 mg/L. Lighting: Not noted. Stock and test solution preparation: Volume/volume direct addition to test vessels. Exposure vessels: Not noted. Number of replicates: two Number of daphnids per replicate: 10 002003 Numberof concentrations: six plus a blank control Water cDihsesmoilsvterdy odxuryignegnt(hfeinsalt)u:dy: 7.5 mg. (control exposure) 7.0 mg/L (3,200 mglL exposure) RESOLTS 0000 N3,o2m0i0namlgicLoncentrations: Blank control, 180, 320, 560, 1,000, 1,800, and Element values: 48-hour ECs) = 2,800 (1,700 8,100) mg/L. Element values based on nominal concentrations Remarks: Testing was conducted on the mixtuarse described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. conclusions 000000000 The FC-600 48-hour ECs, was determined to be 2,800 mg/L with a 95% confidence Interval of 1,700 to 8,100 mg/L. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 p0 atAQ0 uATY 0000000 Reliability: Kiimisch ranking = 3. This study lacks description of the wmeatshnoodtolporgopyeurlsyedchaanradctreerpoirzteidnganodf ttehset sctounddiycltaicoknss.anTalhyetiscaalmpclonefpiurrmiattyion of the amount of fluorochemical proportion in the solution. R0 EFERENCES0000 This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, 1977. omer 0000 Last changed: 6/28/00 o 002004 Ps . BEST COPY AVAILABLE - : 740101 ENVIRAOQNUMAETNITCALTOEXNIGCIINETEYRIWNOGRKLASBHOEERTATORY Ne --------. Type Test 4396 Hours Tocca Sumy Material Tested_FC-00 (21577) RAW Test Organisa_FoshoDsAtP-IsinnMeArSUR Ava. Neigh sAvigt.e ARS iBA r 2Ne Bi Date Started 8/8/71 Date Completed Rll 2 2 Tine Started__1: 30 PM Plotted so 28005, /p Ditution ater Casbon Fitterod - Wout hater (AERATED Ditimod water, Ary % blsLoaeds i L48.a5 ai SLRsin Lm siveFDop.e si 8 pn p. m Se"iNdXeTl ipat LER 2D.0 dahil 45,1:0D.0 2! sed 0 Soa [oo TTToTT 0JE0) 0| J22]No[J4] [1Tes [T1[T7 l209 ] N8 J Cos [0[Te[Te: s lel Xox]C A <5 7)I ENi Fl7 ee TM [LfT Tl A NI 7 1] ; . `omments: 1g, Colentation do 1%B Rzg oR 3 on 100, BE E E eR Re Tes Tea take a i CEE HE ET EEHi Fj HTTEHPEAT ie wial EE HT Sh Pl g BE nn pa TTT Tn CE J g Ee TT aE RHEE E ER R ERET TST HHtElLR b ILtEe pitiedEE Hit ML FHEHE CdiEe El i e 10 JILEITER past S060 70 "80 a oi na R E ECE H HRENNCL[HhEP CHEE He EERI Fe L LH HRE E HTh s hi dE Nan iJelienie isy FCe odeis] Sra n C le L eefA er m oE E me =o Tl Re LHiEri aC hoHEh i H en asS am hE sm HT iLhE a h y a e FHL Hr ai SEGEaliENC SRH a EdhYEoF n n E hh E Hn ea 3:r C nlr leErnehs ePove 12007R TBEil i arH abe 3:E TLE R ITiEiE R TN Hl i E C p i i Aa l Eai H4. rt Em oh a EH EE i ie dai hE LIa NE ma gx oHEen fEehBIH w iinbe dmn Lo psd Ht ph ug SEE Ba a Por BEE HINHELH TE widnooAnRnEneT ieTTHS aiH tH e cE I siEHE Hiill H Hi diE L pLihbE eE aa SEEE EfAaErsa eldai te, IHREHEHH EaEE ii LEE Hiss Hes EERE a r AnH n UE SE ER L fpiihHe He BEST COPYAVAILABLE dy Sif a ei er 5 % EE a EREAEEe a CM L eruh Se TC EL Bt pe me vn Nouys B eBapg ayD lau lo ORd E TL n as Rd EE e uCaateo V os a l S E OER ARE RE SE RE RR LH a TE Te ee xii Ee es nl ces i Hee ae Lo goag srt eh CA WNBW IEE oi ateiiioa s oon sa iocnia. e e pivtn eesste eeel co o 0.719e 260558 eh> Edi meas naER OEE Log a SR \aN BREE RSSEN AA Re a Ghl e CE a RRR Sai a hh BEST COPY AVAILABLE "207