Document LKL0n4nERNn7R98YnxxLxnx57

Snthuhial BIO -TEST 1810 FRONTAGE ROAD NORTHBROOK, ILLINOIS 60062 AREA CODE 312 TELEPHONE 272-3030 Dnc. June 13, I969 Mr. Elmer P. Wheeler, Manager Environmental Health Monsanto Company 800 N. Lindbergh Boulevard St. Louis, Missouri 63166 Dear Mr. Wheeler: Re: IBT No. A7204 - Four-Day Fish Toxicity Studies on Seven Materials We are submitting herewith our laboratory report dated June 13, 1969 prepared in connection with the above study. Very truly yours JCC/kjl J. C. Calandra President DSW 380661 STLC0PCB4100679 I u */ -REPORT TO MONSANTO COMPANY FOUR-DAY FISH TOXICITY STUDIES ON ' SEVEN MATERIALS - IBT NO. A7204 I. Introduction At the request of Monsanto Company, four-day fish toxicity studies in Rainbow Trout (Salmo gairdnerii) and Bluegills (Lepomis macrochirus) were conducted on the following seven materials: 1) Aroclor 1242, Lot #AK-255 2) Aroclor 1254, Lot #AK-38 3) Aroclor 1260, Lot #AK-3 4) Aroclor 5460, Lot #AK-390- 5) Toxaphene ' 6) Technical DDT (2-1/2#), B0903604 7) Halowax 1099, 52 percent Cl \ r.T1ITi""I'llM 380662 STLCOPCB4100680 political B I o - T E S T JaUnaivuei. Due. 2 II. Summary - ./ The results of the four-day fish toxicity studies are summarized below. Due to the paucity of one of the seven test materials (Halowax 1099), complete data are not available and the figures in parentheses represent only range-finding data.^ Test Material i- ur-Day LCjq (ppm) Rainbc w Trout Blue gill Aroclor 1242 ': \ t > 100.0 0.2 0.66 Aroclor 1254 > 100.0 0.66 Aroclor 1260 Aroclor 5460 >100.0 > 100.0 1 - . > 100.0 A ' > 100.0 Toxaphene Technical DDT Halowax 1099 0.0080 0.0056 X. 0.0052 0.0078 (10- ioo) (> 100.0) Respectfully submitted, INDUSTRIAL BIO-TEST LABORATORIES, INC. ' #>Aa'"vJ'vu (\S^s\Aj Report prepared by: Carmen Mastri,' B.S. Section Head Acute Toxicity Department Report approved by: M. L. Keplinger, Ph. D. Assistant Laboratory Director June 13, 1969 QLu69 Otis E. Fancher, Ph. D. Director psh SW 380663 STLCOPCB4100681 hlal B I O - T E 5 T lal^-iaiyiiei, $ttc. 3 XII. Procedure - ' The procedure was generally the same for both bluegills and rain bow trout with each test material. * * * Healthy, young fish with an average length of 35 to 75 millimeters were used as test animals. All fish were kept under observation for a " period of not less than ten days prior to experimental use. During this period, the fish were observed for general health and suitability as test animals. The fish were held in large aerated stock tanks and were fed - frozen brine shrimp until three days immediately prior to testing. The . rainbow trout were maintained at a temperature of 13C and the bluegills at a temperature of 24C. All stock tanks and bioassay vessels contained reconstituted, deionized water. The following compounds were added in the amounts stated per liter of deionized water: ^. . '30 mg calcium sulfate 30 mg magnesium sulfate 48 mg sodium bicarbonate 2 mg potassium chloride ~ - --- . Preliminary screening was conducted in order to find the general level of toxicity before beginning the actual bioassay. Once this level had been determined one to five concentrations were selected for the actual bioassay. Bioassay vessels lined with disposable polyethylene bags were filled with ten liters of well aerated reconstituted water. To evaluate the selected concentrations, ten trout were placed in each bioassay vessel. In the case of the bluegills only five fish were placed in each bioassay vessel in order to insure adequate dissolved oxygen concentration (D.O.) DSV\f 380664 STLCOPCB4100682 fr'dtiirkial BIO-1 ti I alu>ia&u&i., ync. 4 in the warmer water; therefore two groups of five bluegills were tested at each selected concentration. An untreated control group consisting of 10 fish was also employed. After a 24-hour acclimatization period, calculated amounts of test material necessary to produce the desired one to five concentration levels were dispensed into the respective vessels. The test solutions were prepared within one hour of the bioassay. Following the introduction of the test material the fish were observed for 96 hours during which time all mortalities and/or untoward behavioral reactions were recorded. The concentration of dissolved oxygen was de termined for all test solutions in which mortalities occurred, to insure that adequate oxygen was available to the test specimens*. Concentrations of dissolved oxygen of 4 mg/1 (4 ppm) for warm water fish (bluegill) and 5 mg/l (5 ppm) for cold water fish (trout) were considered to be minimal for valid testing. The pH of the test solution was also determined when mortalities were recorded. '' At the end of the observation period all data were collected and ar rangements were made to calculate the 96-hour LC^q employing the technique of Litchfield and Wilcoxon**. * Standard Methods for the Examination of Water and Wastewaters, Twelfth Edition, American Public Health Association, Inc. , 1790 Broadway, New York, N. Y. : Part III, pp. 406-410. J. T. Litchfield, Jr. and F. Wilcoxon, "A Simplified Method of Evaluating Dose-Effect Experiments," J. Pharm. & Exp. Ther. , Vol. 96, No. 2, pp. 99-113. " . DSW 380665 STLCOPCB4100683 jwluddd B I o - T E S T Xa&yiafouei. Vxc. 3 IV. Results No deaths or untoward behavioral reactions were noted among any of the animals in the -untreated control groups. ` A. Rainbow Trout . 1. Aroclor 1242, Aroclor 1254, Aroclor 1250, Aroclor 5460, Toxaphene, Technical DDT, Halowax 1099* ' a. Mortality, DO, and pH Data The mortality, dissolved oxygen content, and pH data are presented in Tables I through IV. * Range finding mortality only. 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' Reactions The reactions noted following introduction of the Aroclor 1242, Aroclor 1254, Aroclor 1260, Aroclor 5460, Toxaphene, and Technical DDT are presented in Tables V through X respectively. os'n 380611 STLCOPCB4100689 JtuLtslwU B I O - T E S T Jal&iaiyuel. Sue. HoPh aM* (M oIS H h 3 S*--Oi . so o t* <; > 'O -t-> oft O w OT p a -Su* < < H o H 2 a w H 0 Eh < .P 2 m H H Ww rt -< Q 1 U P O ti c O -rt Q j ~l 60 " to c O -JH .-I tp* 4> po >-1 rj BB H^ hO '<O P p --I O On H 4-H 2L rMt o rOt og P Q pj 4-> o> c CQ O cn oP MO **H "55 g> is 03 u vh G 05 0 * .aO r< ih Q 1) pG a 3 ea H o 'O Eh Cr, < 'O T3 V-* 4+-)> oo 2 >S >, V 1) >> oo oo a> v Ctf pj oo Tf (SJ <sa r- 60 PH a a P W4oHJ V rt o > +J T3 O Pj S.S P O H4J ) -r ^g s-a o' C o U o o 11 DS\N 380672 STLCOPCB4100690 'jjnJhutual B I O - T E S T jEahyiaiotioi, Stic. o? h in ro * O D i a o r-i y o > y > < -?K w W -4Hu-> < r*( X o Eh Xm H H fa w fa rt H 01 2 o fa co OG X4-> n} 0) mV O Q CO H 4t-f Q o W) g i0j3 H H m u 2 V O GH O X EH rH rH 0 Ey Eh fa y rt V Pi c o vS -- M .H c y u Oc. '-- G O O *0 y -M o co G O u ri 4} Pi O o o o DSW 380673 STLCOPCB4100691 $wiuibual B I O - T E S T Jaky&lyuai, Due. uSo H a*o CM o* U O Hri ctf Hv ou >. TJ < > CO W p < 0X E-t .C m H > ri Q 1 u of Ui *c *2 o Sri oO ^i-i _ P 60 W4 C gj ^ g-SJ a r* O 'O H ^ <; cs -'--<*1 O co 'oM3MrJi ?o*2 fH -M O C 4) O O SSI- O to Li u **i a m ? *$ c jh 63 6 O^ H h <: o 4> O oo> o Cj O CM r- c O 4r->i o o ri o O a, > ffi oc Si> ! si o o u e o U *** " r 7 r -*ll i 13 DSW 380674 STLCOPCB4100692 S/ichcibual B I O - T E S T Jal&iaivu&t, Sue. oS> h Eh o* vD o Hf m *c ri u o H o o a *--l u < a > wA a< 2 < Eh w Eh m C O < 2 & Eh W >ri WQ Eh i u 3o o O rH o 13 gM> m r* .- c m 3 O o vs < a c-- 4orH-*i ^ O 3J o a Jj d2 Ah Q pJ 4~> a c oO CO CD G p Q H 4-J ctf OU feO 4-> c 03 O H pH . (U o pH o H pH T3 <H Ct, 73 U 0o c o 1a o C o U o o o 14 DSW 380675 STLCOPCB4100693 * ' Suetudnial B 1 O - T E S T o0 h H * o av P 0) s *H n! Pi a) X o >* H *a P4J 8W >* W P H oH X <o E-< H tn r 0 c Vn0 On *-< 4J P P Ctf O to 4-> Pv-i CQ O H i-t CQ 3 O 4> 0 sH G rC fi H O E-i fr, < 00 00 f\l t 1 . '>* M N Ag 15 "S 15 <v V 0 Ppp rj H rH rl H H CCc PPp vO rj rj I 'P -4o- O X <n cC oO CJ*H 4-> +H-> o ri nJ VV Pi Pi o P 0 H a4-> 1* u P o V vO in o o > >- rH <H M >> > Vi V-> - -*-i 0 u .u O rt rt u n! >1 rt P oa. >> V 0* >. V cu >* O O, >. XXXK o --( 00 cv3 CO oin oooO oo 15 3806T6 osnn STLC0PCB4100694 fnduibual. B I O - T E S T Xaklai&uei.. Sue. 16 o3 M o*s H ri P< >N *0 X 4J W OT < +>j> oHH Xo mH h <s Q u 0 o fa A c* *J O f-o SQ "* M i <n m C ij O -r* 3 C S 0-2| n 7* O 'O E* fa < e-- O <o d o 0h O f<Ul S Q CU ^ o CoJ ^ flS wo 6CJ0 *H ^-1_ <rnj 2w 0O 0) C JC3 fi o5 H fa c oo I fM r-i <M 00 t^ 00 * C<J* > > OO TV3 Ph o & rt V Q & oi vOI '4OV-> o % (0 c roM^ U <Tj 0) CoJ 4- o d V c4 o 2; c 0H s 1 01 U C o U t-- o o o THIU-3 . T-H<-3U> oo 0) 01 cG OO V. u "+oj d nJ V <0 Oi Oh oO 2 00 fM CO oO pO oo - 4Sl--Jk1 _> u Oud <a0, >> K 4*>hJ* rj >O -oM XJj du 2 fl) 01 Oh O >H U ffi Oh vO m o --1 oo oo dS\N 380677 STLC0PCB4100695 SnfLid'ual B I O - T E S T lahyialyuel, Sue. u . B. Bluegills . 1. Aroclor 1242, Aroclor 1254, Aroclor 1260, Aroclor 5460 ' Toxaphene, Technical DDT and Halowax 1099* a. Mortality, DO, and pH Data . The mortality, dissolved oxygen content, and pH data . > . are presented in Table XI through XVII respectively. , * Range finding mortality only. DSW 380678 STLCOPCB4100696 StuL'tiUicl B I O - T E S T lal&ialcsd&L. Sac. 18 , ffi ffi ^ ffi^O a. P d u ~ re e, pj 6a pi o 3p p .i IT* oso dp V H> O *4 X cn a** p H bo Nv r- PM a M r--I 4dJ d ffi h O P PJ K O O U d B co K a d p i5 o J. p > d p VH O t> XP 0 Eh tN uV oP fa ACO H Ui 4d-> h rQ u H to W >* d P 1 o ,Q -u S I* Eh h oP fa 3^-S o Eh 5 P <M t~ vO r~- oooo o oooo vO O' `I o r- f5 oo i a " U3 O 1 K'-r i|23 ** o * po H 4-* rt -p u _ *. 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Vh O 2w dl ' n avO in H P o o d o d d ir _d4 o in U P d o U -U >S d dd 3S" 0g3 * p, mX dd ^d ^i P dd d da +> CO d M( U 3o M d Pi in dd o Po ^ unjfi^iW-- iwi.w^.aw DSW 380683 STLC0PCB4100701 O | w/ - I * a r TE S T M A T E R IA L : T echnical D D T 24 48 72 96 .* ffi * bS a, P 4) fi- -OM 45 U id fi o ?xva o P o to fi 4) . 00 M V J > E xr O . N* *o 45 > O h K. ffi CM 05 *** H *o P -> ro r- id 4-> id P >* *3 ffi P< 2 w o fi id O xQ 0 Eh ,fi <Hn t* o id s P 1 h K, K i5 O Z, p fi V Uo ho V Pi u 4) .fi B 01 45 3 Eh o --< o r4 o o r4 O H 4f)i 45 C5 h 45 P. f-4 o o O *3 45 Id O 45 C5 B h 3 h 5 OQ id S' H -P ff-ii Q co c3 ~l o V .M wd "oj 0) o >o i0d2 -<43 CD 2 .S-! g h 4) .O a id - fi 4) o o o o 1 m 45 A 02 3P 2; oS n fi 45 r4 -M oooo 1 fi 1} -1-4 05 .0 lo o o o o' 1' g Pu fi 45 a, to 45 to to c> h 14 45 3 .O O oooo I S ooopo [Z - H o 1 o _o o o o a. * P4 CO r~ o o 05 X Id 45 ^ 45 --4 ?4 o iHd id ii >4 01 0 45 fi * c ow 4-* 00 IM vO id -- H r4 m >n 4PJi 3 C5 a CL u o o o o o o o o o H. oo ' oo m 1- V 13 P _ **> >* id c 45 0 P 1 **. 0 **' d u .fi O c -- tt H O o- ....... i. * -o O fl* 23 DSW 380684 STLC0PCB4100702 B I O * 1' t b T Xab&ialortU. Vnc, to 9 ~O' 2 s .O' CQ 3f B ow H> X w A X o H PQ < H tn fa tco --o c< fa o tcrot p; E kS.o PQ *d SM ?w pp d 4> . tO>H X cWT* K ol oo "o^Q o > Ho o(0 u W^ M5P QN O rf E Q ^ Pl E e p i o o J. Q d u 4CJ mo O o y. 4) Q fa 4dUJ o 2 o *0 ^ +5 ds m CM h o ,o a a 2 o O' d , CrM- Q CO S is* Lq o E 'tf 3 CM I* 'OI *| P o 4- d -p ^ Pl cS SPJ o d o U O o CM o o o d oa*J o d d o o CD ? 5 4-> d 4) O h' 4> P "'S *8* 4) fi g h $> Ou CD 9d s+*3 O' r< P' S 5 S' CD fe O 4) r--i > d d 03 rt CQ d do 1o-1 oTO VH 4) 5 O O r4 4-> d d *H 5S0 9 -d 41 CO P 0) CO 9 pU pP CO X d9 o o 29 U H dd A U CO 0 in 9C O H Ud Jk d Q1 a<a -*-> 9 -i-t 9 o 9d Ao a, HU, o fa 64 DSIV 380685 STLC0PCB4100703 v r* r *r S'rtdutlual BIO- 4 U I n t. U. / ., 25 k. . Reaction ' . The reactions noted following introduction of Aroclor 1242, Aroclor 1254, Aroclor 1260, Aroclor 5460, Toxaphene, and - Technical DDT are presented in Tables XVIII through XXIII respectively. oS\N 380686 STLCOPCB4100704 yttdu,UAial d - * c j i j.atMyuutyuc*, ync. ?Eh- ^O '< fM 60 0 <vj fH l 1 D 'O>. o w o J >- V Ci o H X VM o o Eh >U E-< W Eh .CQ ct >d W W Q I W Eh o Pm o O 4-> Ort V P o4) m a '4Od-H> Vw O 60 +ouj 4) 6H Eh h <; 0] fn O o d 4) At V Q i H4 N (M 4oH- d -p 00 >} h pH CO in a O- & o o o o o O U dsVj 38^ STLCOPCB4100705 'fttdiahial B I O - T E S T Jal&ia&vue&. Stic. 27 43 03 o Q oo CQ H * o H H o Ixi O 03 rf V. hCli 3- ^o ofOdH Xo Q 5e*$ --I 0h3 O 4) 4) I 6! 03 O oCO 5 <d 03 <-l o 00 4_> 03 jJ o 43 .5 ^3 s - O 'O o O' ii (SI r~i 00 CO fsl sj< & a 43 Q fl- sO <# ' CS3 (M . o 4-> o ri 43 Pi o s? sa o' fl oU O +4J3 o 2 03 a o **H-> o d 43 Pi O 2: H o 'O T3 43 4-> ` +43j O0 22 03 CQ OO <HP v> oo d 43 43 Pi Pi oO Z 5* * > H +o* .rSt <4 U Md 43 O < O-i >s >> KK CO (SJ vO r-H CO 1C 4 o oo >S 1-1 > a nl 0 Dh >> K DSW 380688 STLC0PCB4100706 - Jfictuil-ual B I O - T E S T lalota&yueA. 9hc. bo vO 4) d H h X r-0H o o t* < o du w X H-4-> 2W (Q A < o H X o H w H ,do < S > (4 E-< co Q Wh E- od Ph e o .2 {3 bO *1 a d O tH d -d d o ,d 3s rH O TJ ^k< Od a"I T! <4 * o M <4 g a a13 -m1)0 v o> 'C 4o<Ju u V di o oo C OH oo (4 V <4 O CU K d 4OHJ 2 ta o d o U o o 28 DSW 380689 STLC0PCB4100707 . " 'Jwhtihtel B I O - T E 5 T labyia&ual. Vac. J " C trJi O? oh 3 Q ~J " 60 h ~ c O o T** -r< a 88 pH O "V ^h< uP o o vO 60 4) 3 tn H X X b O poH ou < a >* *o C o 4PJ w o AVS Pi w o (a Xo < 2 o H H w .0 >u> aS E- IQ h >> w H OT a Q Wu E-* P o c: c H O On 4H^ hH-> Lij AS * O ' * O AS O o 6l Q Pi o o4) CCQ a o to _ <n O *ClH-QsQ l V 8: oHhc c o o u AS AS o so. v K c o 4HJ S-g. s9 o' C o U o o T'.nv.,?.g'sife 29 DSW/ 380690 STLCOPCB4100708 30 - 0S\N 38069^ STLCOPCB4100709 - *' TSnJuiUld B I O - T E 5 T labviaZyueS, yne. c S..2 oVM to- 4^- CD vI 8a 8H w O V in Ui < IC <M -- - H Q <H M y H3 -- Q -n HdHo i Sm o ca C O ( an X X H a s fHSu 0) H a < 5 w H -M w *H oHOvn in aMa yrf y a VM O >urt 8 P w H w cd P i W *4 G o p . O5 ^ *rl ** gt<j O cyj O_ 3 5 Q eJ y Vo o05 G W& Xrt H M oVM fn CO y 8M H oI x) -yM - x) 4y- ` t-*yJ-> ooo 2 G 4y-> o c *4oH-> o d (D . 05 g 4OH-> u <d y Cd 05 4GoH-J y cyd 05 G 05 G 4>-H>* 4OH-> 4OH-> _ 4M>-> yuV cyd - <d y CJ Pi d o oooo XXXXX c o 2? 00 <\J sD M o M o ro O m o pi !a o o o o u' o o o o o c o o W! DSW 380692 STLCOPCB4100710