Document LJzbdOLKGvy9QDxKGME0g78ob

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA tetrabutylammoniumsalt,Ammonium perfluorooctanoatePFO, FC-1 16, FC-1 26, FC-1 69, FC-1 43 or as a major component of FC-1015 or FC-1 015-X. (Octanoic acid,pentadecafluoro-a,mmonium salt,CAS # 3825-26-1) Remarks: The testsample isFC-1 015-X. It'psuritywas notsufficiently characterizedt,hough currentinformationindicatesisa 30% straight carbon chainversionofFC-143 in80% water. The 3M productlotnumber was "HOGE 205". The followingsummary applies to the testsample as a mixture of the testsubstance In water solution with Incompletely characterized concentrations of Impurities.Data may not accurately relatetoxicity of the testsample with thatof the testsubstance. Data was used to compare toxicityof the branched(straightchain ammonium perfluorooctanoate homologue mwure inFC-143 vs. what Is supposed to be the 100% straightcarbon chain ammonium perfluorooctanoate InFC-1015-X. METHOD Method: OECD 209 Test type: Staticacute GLP: No Year Completed: 1996 Analyticalmonitoring: Dissolvedoxygen concentrationasnd pH. Statisticamlethods: Not applicable Test organism source: Activatedsludgemixed liquorcollectefdrom the Metro Wastewater Treatment Plant,St.Paul,MN Test condition: Dilutionwater: MilliporMeilli-CFwmater Synthetic sewage: per OECD method Reference and testsolution preparation: A stocksolutionofthe referencesubstance,3,5-dichlorophenolw,as prepared by dissolving500 mg in1OmL 1N NAOH, dilutedto30mL withMillipore Milli-OT'A water,then brought tothe pointofincipienptrecipitation with1N H2SO4 and dilutedto 1L withMilli-C@wmater. ThepHof the referencesolutionwas measured to be 7.2. Test substance was added directltyo testvessels. Test vessels: Not stated Numberofconcentration4sp:lus1 referenscuebstancaend2 blankcontrols Temperature: 19.1- 22.1C Totalsuspended solidsand pH on day of testing:3.22g/L (TSS infina1l.3g/L)and initipaHl 7.8and finalpH 7.9 Element Basis: Respiratioinnhibitiaosndeterminedby oxygen consumption. Remarks: Values correctedto 20C forcalculations RESULTS Nominal concentrations: 2 Blankcontrols4,20, 840,1660 and 3320 mg/L Elemeritvalues: - 30-minuteEC50 = >3320 mg/L 3-hourEC50 = >3320 mg/L - Element valuesbased on nominalconcentrations. Remarks: Testingwas conducted on themixtureofthetestsubstanceas describedinthetestsubstanceremarksfieldT.he valuesreportedapply tothatmixtureand notthetestsubstance. CONCLUSIONS The FC-101 5-X 3-hourEC50 foractivatedsludgerespiratioinnhibitiwoans determinedtobe >3320 mg/L. Submitter: 3M Company, EnvironmentalLaboratoryP,.O.Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcriterifaorquality testingb,ut lacksanalyticaclonfirmatioonftestsubstance concentrations. There isa lackofcharacterizatioofnthetestsample. REFERENCES This studywas conducted by the3M Company, Environmental Laboratory,St Paul,MN, Lab Request N2169. OTHER Last changed: 5/25/00 ENVIRONMENTAL ACTIVATED SLUDGE MIXED LIOL)OR Type Test OC-CO kr-'T[VrArF-oSLUC)C-r- iZC-SIPIP.Pk-TIOO 7r "LR Date 6 Analyst page of r% -I s-@ ACTIVATED SLUDGE MIXED LIOUOR 6'a"t'o'@@6b-'t-'ai-q'@ne-d:Ls-95 Date Used 14--15- Mlied L@@d' Suspended Solids(MLSS) 2:;@,o g& Adjusted to'. M LSS':' 'fiAal@'t'est'solution"'3 g/L Initiapl)4 -7 (I*sttestsoin. prepared) FinalpH .-.,-,.,Comments,' g/Ll (last"iolpnr.e*parso Sample D escripilon InitiaplH of highestconc. tested(33ab-.elL) comments -7 'a. REFERENCE SUBSTANCE ampi --@,"IniptHial-ofstock,sblutionSco mgtl) '7- ::,@@,@,Adjustet.odpH, Adjusted to@'PH, OSURItCONDff(i rat;d LR ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIONTEST SM TEST SOLUTIONS PREPARATION Test Substance: Saifiplo tois->< Description:,C-Iffck%l I I Analyst: v-s Date: 44 25 -c? Test Conc. ffVadded rma/L per 500 mL WL q -zc) C? -Yqo q2o 33RO ",X CIQ -T-30 ML MO(l) water mL syn.sewage 22r Soo mu feed per500 mL mL microbial inoculumt)er 14 g rq -2 4!@-o -2C--)O- -zoo -elk TotWV61unio@.'.@;"w ML C.C)> --.-.-- tt zz 7-" ENVIRONMENTAL LABORATORY LR lp CECO ACTIVATED SLUDGE RESPIRATION INHIBITION TEST =9 RAW DATA @.Test Substance: F'C- Source of Activated Sludge Mixed Uquor: Date Receiv@. Analyst: rle,@4"V V,-i kc, Date Used 30-MINUTE EXPOSURE TestSubstance :,,Concentration o.s SK CONTROL I 4;tr-, 5,fqc 3.-:5A C, 7.3 DISSOLVED OXYGEN CONCENTRATIONrh TIME-MINUTES o@ Tomp l..O I.S 2.0 2.5 3.0 3...S 4 " '4.S 5.0 .,S.S, '7. @6 C-.? S.-+ 5.-3 (,-.g (,.q .-L s (,.0 5.16 5.1 sq 5.1 4-1) 4.14 -12- 5.r S.-S c. 5.'3 &f.% 3.1 q,9 4.53,S S.,7 li-o -3.1 5.q 4.1 3.(. I-1...V so 6.t ;@6 26@",T Aa-. I 7%W SK CONTROL I f.. (p.4 (..o @-HOUR EXPOSURE DLQWLVF-D OXYGEN CCNC-.... Test Substance Concentratio(nf!llw O.S. -1.0 TIM E - MIN ES 2. 0,-, 'o q 14'2 .9'4 -@OKCONTROL pH.ofactivatesdiu mixed.,I, 'PH..g,!@higttecc fi'to attire: iti@l iual 1.3 wo*wMm 7 EW EST)LTS: OECD ACTIVATED SLUDGE RESPIRA'NON INHION TEST 2 09 Lr P1828 Date:4/25/9 oot--Lwatance: FC-1C)IS; Tr4 f'Stiefenhoefe-@r.r 30-Min o@,Exp si,re MGA.IV 0.6 'I.0 2.0 2.5 j@ i@. wm BKI r,.- '(7.6 7.2 6.8 6.5 @420 @ -@t7A I-!@ @.1 8.8, 6.3,@- 5.9 i840 -t6@.4 6.7 5.3 14.9 1660 -B.O, 7.8 7.6, ,7.3 7.0 3320 173 6.8 6.4 6.2 5.8 ni M'Vod ()XvienConcentraflonms3./L 3.0 3.6 4.0 4.5 5.0 5.5 ow, mm saw, 6.0 6.6 5.3 4.9 4.6 4.1 5.4 5.1 4.8 4.5 4.0 3.6 -4.4, 4.2 -3.9 3.6 3.1 2.6 6.6 6.4 6.0 5.7 5.4 5.0 5.3 5.0 4.7 4.4 4.1 3.8 To". Ic R (1) Ra % lnh. 20.6 0.7 61 0.7348 20.9 0.7721 0.7253 0 20.8 0.7178 0.6788 6.1 20.7 0.6167 0.5874 18.7 22.1 0.6752 0.5834 tBKKJ2 -1,6.4-v6.0 5.6 6.2 4.8 4.6 4.1 n.control(s4),,'3,4 CI)a> 3 2 Eip 0.5 i.o DissolvedOxv ion Cornwcontroans, ma/L 2.0- 2.5 3.0 3.5 4.0 4.5 1 5.0 1 5.5 7.9@ 0-7.7- 7.4 t 4.7.0 6.7 6.4 6.0 5.6 5.3 4.9 4.6 4420, 7.0 @16.7, 6.6,; @ 0.1.- 5.7- 5.3 4.9 4.6 4.2 3.9 3.5 i'84O@ '46.0 6.7t@ SA'- @16.0 - 4.6 4.3 3.9 3.5 3.3 2.9 2.5 -1660 6.4 6.21 5.9 - 6.6, 6.3, 5.0 4.7 4.3 4.1 3.8 3.5 8.7-L@ vB.St 4 8.3'0 6.1 :7.9 '7.7 7.6 7.2 1 7.0 6.8 0.6 20.9 0.7564 0.7105 RaCm (6): 0.7226 *0 19.8 20.4 20.5 20.7 20.6 R (1) Ra (2) % lnh.(3) 0.6982 0.7297 0.7091 0.6036 0.4291 0.7080 0.7097 0.6849 0,5750 0.4059 0 2.9 18.5 42.6 @iO.6I)t@8.2 ,*7 9,0-, 7 7P n@ Ec 0(96%'@CI)a> 3,3a 6. RaCm (5): 0.7056 ENVIRNOMENTAL LABORATORY LA Cr, OECD ACTIVATED SLUDGE RESPIRATION INHOITION TEST 8 209 TEST SC)LUTIOTS PREPARATION Test Substance: Sample Description:AlArl c. Analyst: Date: Stock Solution Preparation: IL 1 SOOV."A -IDR-P ip,%j wc,,-. LABQL !220 GAoc- Test Com maA- @A rnL added gmr 500 rnLMO(l) water mL syn. sewage 2or 500 ML- 122d-RlLjiQ2-ML mL microbial inoculurnoer 500 rnL TotalVolume mt a'f IL4 ------------------ c, -.VOW 7 ,Oet;p LR ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIONTEST,0209 RAW DATA Test Substance:- C5 Source of ActivatedSludge mixed:Uquo r: AA4 Date Received: Q5 Date Used. Analyst: 30-MINUTE EXPOSURE Test Substance Concentration 0.5 BK CON7PO- 1 IC 01=LVED OXYGEN CONCENTRATICNS..,616t', TIME-MINUTES 1 5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 Tamp 5.5 cc 7.(. 7 06 q (@.T. S.1 6 4.1 9LO E;.,t S.L, BK CONTROL I L4 -T 3.-4 s.q ;to.1 :3-HOUR E) TestSubstance Concentration AK 01=LVED OXYGEN CCNCENTRATIONS.;..- T IM.E.,M-INUTES 0 5 1 0; '1'S' :'20 2.5 3 :9.0 N-W WOW WM i*w ,-Alvwwtow""" *N&t& @A@ 4*W W$M VON* lemp 0 S.S. oc, c ao@ *O"k Attt. actIva 0 iquo InIli na- concen iid IWIUS him" OFR Ua a 'OECD ACTIVA'TED SLUDGE RESPIRATION INHION TEST, 209. Lr Pis;, Date:4/25/9 30-Minut x 7.9 7.8 Diii'olve0d 3.0 an Coricentrationms L -4.0 4.5 5.o .5 Ic R 2 Ra (2) % inh. llq I BK2 .8-I;Ya. 6.0, 'cuff.-:I'Cnontrols .4 , .2 4.8 4.6 4.1 1 IJ A 3-Ho I -WL_l U.5 1 1.0 I,iI- KI; -"7.0 9.2 1 @-2 Di molved T --r m .0 L 5.5 .9 4.6 9.0 8.8 8.7 8.5 8.4 8.4 8.1 Tom 1 .8 RaCm (5): 0.7226 R Rs 2 % inh.(3) 0.6982 '0.24 6 0.7080 0.2386 66.2 6.6- 4ja?.'2 In 5.9 6.6 19.1 0.6606 RaCm CSy 0.7056 "Y